MD10G1260100.v1.1

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011877
Physical Location & Seq
Reverse (-)
35384300 .. 35388988
4689 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1260100.v1.1.491

Sequence Viewer

Length: 1437 bp
ATGGCGAGCAGGAGATGTTTGCAGCGTGCGCTGAGCACATCGAGCTTGAAGAAGAGAGTGATTAGGAGGAGCTTCGCTGCTGTCCCTCAAGCCAGCTCCCCTTCATCTTCGCAGGAGCTCATCAACTCTGAGTATCAATACAGCGCCCACAACTACCACCCAATTCCCATTGTGTTTTCTCAAGCAAAAGGATCATGTATACAGGATCCAGAGGGAAAAAGATATCTGGACTTTCTTTCAGCTTACTCTGCTGTTAATCAGGGACATTGCCATCCAAAAATCATCAAGGCATTACAAGAACAGGCAGAAAGGCTCACCCTTAGTTCTCGGGCTTTCTATAATGATAGATTTCCAAGATTTGCCGAGTATCTAACAAGCATGTTTGGCTATGACATGGTGCTGCCTATGAACACTGGTGCTGAAGCTGTGGAAACAGCTTTAAAGGTGGCAAGGAAATGGGGTTATGAGAAGAAAAAAATTCCAAAAGATGAGGCTATTATTGTCTCGTGTTGTGGCTGCTTCCATGGTCGTACTTTGGCAGTCATCTCCATGAGCTGTGACAATGAAGCAACCCGTGGTTTTGGACCCTTGTTGCCTGGACATCTTAAAGTTGATTTTGGTGATGCAGTTGGCCTTGAAAAGGTTTTTAAAGAGAATGGAGACCGCATAGCTGGATTTCTCTTTGAACCCATTCAAGGAGAGGCTGGGGTTATAATTCCTCCAGATGGTTATTTGAAAACTGTTAGAGATCTGTGCTCGAAATATAATGTTTTAATGATTGCTGATGAAATACAATCTGGTTTAGCACGGTCAGGAAAAATGCTTGCTGTTGATTGGGAAGAAGTTCAGCCCGATGTAGTGATACTAGGGAAAGCATTGGGTGGTGGAGTGATACCTGTAAGTGCGGTGCTTGCAGACAAAGACATAATGCTTTGTATCCGACCAGGAGAGCATGGAAGCACCTTTGGGGGTAATCCGTTGGCTAGTGCAGTTGCTGTTGCCTCACTAGATGTAATCAGACATGAGAGACTTGCTGAAAGATCAGCTGAAATGGGACAAGAGCTTAGGAATCAGCTAGTCAAGATTCAGCAGCAATTCCCAAACTACATAAAGGAGGTTCGAGGAAGAGGTTTGTTCAACGCTGTGGAGCTCAATAGCAAGAATTTATCCCCTATTTCTGCTTACGATATCTGCCTAAAGTTGAAGGAGAGAGGAGTTCTTGCCAAGCCAACACACGATACTATTATCCGATTGACTCCTCCACTCTCGATAAGTTTGGAGGAAGTCCAAGAAGGATCAAAAGCACTTTCAGATGTTCTGGAACATGATCTACCAAAGATGCAGAAGGAAAAGCCCGAGAACACATCTTCGACTGCTTCTAACATATGCGACCGTTGTGGGCAAAACTTGTATGATTCTACACACAACGATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002376 GO:0003674 GO:0003824 GO:0004587 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006520 GO:0006525 GO:0006527 GO:0006536 GO:0006560 GO:0006561 GO:0006591 GO:0006593 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0006972 GO:0008144 GO:0008150 GO:0008152 GO:0008219 GO:0008270 GO:0008483 GO:0008652 GO:0009056 GO:0009058 GO:0009063 GO:0009064 GO:0009065 GO:0009084 GO:0009605 GO:0009607 GO:0009617 GO:0009626 GO:0009628 GO:0009651 GO:0009814 GO:0009816 GO:0009987 GO:0012501 GO:0016053 GO:0016054 GO:0016740 GO:0016769 GO:0018130 GO:0019544 GO:0019752 GO:0019842 GO:0030170 GO:0031974 GO:0033554 GO:0034050 GO:0036094 GO:0042538 GO:0042742 GO:0042802 GO:0043167 GO:0043168 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043648 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046394 GO:0046395 GO:0046483 GO:0046872 GO:0046914 GO:0048037 GO:0050662 GO:0050896 GO:0051179 GO:0051640 GO:0051641 GO:0051646 GO:0051704 GO:0051707 GO:0051716 GO:0070013 GO:0070279 GO:0071704 GO:0097159 GO:0098542 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901605 GO:1901606 GO:1901607
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 ko01110 ko01130 map00330 map01100 map01110 map01130
Pfam Domains
Protein Families
Aminotran_3

Protein Analysis

479

Amino Acids

52.65

Weight (kDa)

7.55

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_3 PF00202 47 - 440 4.2e-125 Aminotransferase class-III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011877)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46180
fragaria_vesca FvH4_3g05240 FvH4_3g05240 FvH4_3g05240 FvH4_3g05240
malus_domestica MD05G1281300.v1.1 MD10G1260100.v1.1
prunus_persica Prupe.4G083300_v2.0.a1 Prupe.4G083300_v2.0.a1
pyrus_communis pycom10g21610
rosa_chinensis RchiOBHm_Chr5g0014471
rosa_laevigata RLG00000032113
rosa_multiflora Rmu_sc0004194.1_g000003
rosa_roxburghii Rroxscaffold_1G00062100
rosa_rugosa Rorug05G0018400
rosa_samantha Rh5AG113500 Rh5CG122000 Rh5DG109300
rosa_wichuraiana Rw5G009850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 713
AccI GTMKAC 1 cut(s) 199
AciI CCGC 2 cut(s) 664, 905
AclWI GGATC 4 cut(s) 199, 200, 213, 1303
AcsI RAATTY 2 cut(s) 477, 1162
AcuI CTGAAG 1 cut(s) 441
AfaI GTAC 1 cut(s) 532
AfiI CCNNNNNNNGG 3 cut(s) 640, 695, 725
AgsI TTSAA 7 cut(s) 49, 638, 686, 695, 736, 1138, 1204
AjnI CCWGG 2 cut(s) 595, 943
Alw21I GWGCWC 4 cut(s) 38, 120, 758, 1152
Alw26I GTCTC 3 cut(s) 508, 654, 1021
AlwI GGATC 4 cut(s) 199, 200, 213, 1303
AlwNI CAGNNNCTG 1 cut(s) 995
Ama87I CYCGRG 2 cut(s) 327, 1355
AoxI GGCC 1 cut(s) 631
ApeKI GCWGC 5 cut(s) 22, 77, 400, 516, 1090
ApoI RAATTY 2 cut(s) 477, 1162
Asp700I GAANNNNTTC 2 cut(s) 690, 843
AspLEI GCGC 2 cut(s) 31, 146
AspS9I GGNCC 1 cut(s) 584
AsuHPI GGTGA 2 cut(s) 307, 632
AvaI CYCGRG 2 cut(s) 327, 1355
AvaII GGWCC 1 cut(s) 584
BamHI GGATCC 1 cut(s) 205
BanII GRGCYC 2 cut(s) 120, 1152
BauI CACGAG 1 cut(s) 505
Bbv12I GWGCWC 4 cut(s) 38, 120, 758, 1152
BbvI GCAGC 5 cut(s) 34, 64, 387, 503, 1102
BccI CCATC 2 cut(s) 279, 719
BciT130I CCWGG 2 cut(s) 597, 945
BciVI GTATCC 1 cut(s) 947
BcoDI GTCTC 3 cut(s) 508, 654, 1021
BfaI CTAG 4 cut(s) 866, 984, 1007, 1076
BfoI RGCGCY 1 cut(s) 147
BfuI GTATCC 1 cut(s) 947
BglII AGATCT 1 cut(s) 748
BisI GCNGC 5 cut(s) 23, 78, 401, 517, 1091
BlpI GCTNAGC 1 cut(s) 32
BlsI GCNGC 5 cut(s) 24, 79, 402, 518, 1092
Bme1390I CCNGG 2 cut(s) 597, 945
Bme18I GGWCC 1 cut(s) 584
BmeT110I CYCGRG 2 cut(s) 327, 1355
BmgT120I GGNCC 1 cut(s) 584
BmiI GGNNCC 2 cut(s) 207, 586
BmrFI CCNGG 2 cut(s) 597, 945
BmsI GCATC 2 cut(s) 613, 1329
BpmI CTGGAG 1 cut(s) 705
Bpu10I CCTNAGC 1 cut(s) 1064
Bpu1102I GCTNAGC 1 cut(s) 32
BpuEI CTTGAG 2 cut(s) 72, 165
BsaI GGTCTC 1 cut(s) 654
BsaJI CCNNGG 2 cut(s) 523, 574
Bsc4I CCNNNNNNNGG 3 cut(s) 640, 695, 725
Bse1I ACTGG 1 cut(s) 418
Bse3DI GCAATG 1 cut(s) 265
BseBI CCWGG 2 cut(s) 597, 945
BseDI CCNNGG 2 cut(s) 523, 574
BseGI GGATG 1 cut(s) 271
BseLI CCNNNNNNNGG 3 cut(s) 640, 695, 725
BseMI GCAATG 1 cut(s) 265
BseMII CTCAG 2 cut(s) 23, 120
BseNI ACTGG 1 cut(s) 418
BseRI GAGGAG 3 cut(s) 82, 1227, 1248
BseXI GCAGC 5 cut(s) 34, 64, 387, 503, 1102
BseYI CCCAGC 1 cut(s) 704
BsgI GTGCAG 1 cut(s) 1008
Bsh1285I CGRYCG 1 cut(s) 1393
BshFI GGCC 1 cut(s) 633
BsiEI CGRYCG 1 cut(s) 1393
BsiHKAI GWGCWC 4 cut(s) 38, 120, 758, 1152
BsiHKCI CYCGRG 2 cut(s) 327, 1355
BslFI GGGAC 3 cut(s) 68, 276, 1068
BslI CCNNNNNNNGG 3 cut(s) 640, 695, 725
BsmAI GTCTC 3 cut(s) 508, 654, 1021
BsmFI GGGAC 3 cut(s) 68, 276, 1068
BsnI GGCC 1 cut(s) 633
Bso31I GGTCTC 1 cut(s) 654
BsoBI CYCGRG 2 cut(s) 327, 1355
Bsp1286I GDGCHC 4 cut(s) 38, 120, 758, 1152
Bsp143I GATC 7 cut(s) 191, 205, 748, 1040, 1295, 1327, 1429
Bsp1720I GCTNAGC 1 cut(s) 32
Bsp19I CCATGG 1 cut(s) 523
BspACI CCGC 2 cut(s) 664, 905
BspANI GGCC 1 cut(s) 633
BspCNI CTCAG 2 cut(s) 24, 121
BspLI GGNNCC 2 cut(s) 207, 586
BspPI GGATC 4 cut(s) 199, 200, 213, 1303
BspTNI GGTCTC 1 cut(s) 654
BsrDI GCAATG 1 cut(s) 265
BsrI ACTGG 1 cut(s) 418
BssECI CCNNGG 2 cut(s) 523, 574
BssMI GATC 7 cut(s) 191, 205, 748, 1040, 1295, 1327, 1429
BssNAI GTATAC 1 cut(s) 200
BssSI CACGAG 1 cut(s) 505
BssT1I CCWWGG 1 cut(s) 523
Bst1107I GTATAC 1 cut(s) 200
Bst2BI CACGAG 1 cut(s) 505
Bst2UI CCWGG 2 cut(s) 597, 945
Bst4CI ACNGT 3 cut(s) 742, 810, 1394
Bst6I CTCTTC 2 cut(s) 47, 1120
BstC8I GCNNGC 5 cut(s) 7, 27, 94, 825, 912
BstDEI CTNAG 4 cut(s) 32, 129, 320, 1064
BstDSI CCRYGG 2 cut(s) 523, 574
BstENI CCTNNNNNAGG 1 cut(s) 638
BstF5I GGATG 1 cut(s) 271
BstH2I RGCGCY 1 cut(s) 147
BstHHI GCGC 2 cut(s) 31, 146
BstKTI GATC 7 cut(s) 194, 208, 751, 1043, 1298, 1330, 1432
BstMAI GTCTC 3 cut(s) 508, 654, 1021
BstMBI GATC 7 cut(s) 191, 205, 748, 1040, 1295, 1327, 1429
BstMCI CGRYCG 1 cut(s) 1393
BstMWI GCNNNNNNNGC 5 cut(s) 28, 42, 248, 384, 911
BstNI CCWGG 2 cut(s) 597, 945
BstNSI RCATGY 1 cut(s) 382
BstSCI CCNGG 2 cut(s) 595, 943
BstV1I GCAGC 5 cut(s) 34, 64, 387, 503, 1102
BstX2I RGATCY 2 cut(s) 205, 748
BstYI RGATCY 2 cut(s) 205, 748
BstZ17I GTATAC 1 cut(s) 200
BsuI GTATCC 1 cut(s) 947
BsuRI GGCC 1 cut(s) 633
BtgI CCRYGG 2 cut(s) 523, 574
BtsCI GGATG 1 cut(s) 271
BtsIMutI CAGTG 2 cut(s) 411, 1432
Cac8I GCNNGC 5 cut(s) 7, 27, 94, 825, 912
CaiI CAGNNNCTG 1 cut(s) 995
CfoI GCGC 2 cut(s) 31, 146
Cfr13I GGNCC 1 cut(s) 584
Csp6I GTAC 1 cut(s) 531
CviAII CATG 8 cut(s) 195, 379, 394, 524, 550, 953, 1022, 1325
CviQI GTAC 1 cut(s) 531
DdeI CTNAG 4 cut(s) 32, 129, 320, 1064
DpnI GATC 7 cut(s) 193, 207, 750, 1042, 1297, 1329, 1431
DpnII GATC 7 cut(s) 191, 205, 748, 1040, 1295, 1327, 1429
DraI TTTAAA 2 cut(s) 441, 649
Eam1104I CTCTTC 2 cut(s) 47, 1120
EarI CTCTTC 2 cut(s) 47, 1120
Ecl136II GAGCTC 2 cut(s) 118, 1150
Eco130I CCWWGG 1 cut(s) 523
Eco24I GRGCYC 2 cut(s) 120, 1152
Eco31I GGTCTC 1 cut(s) 654
Eco32I GATATC 2 cut(s) 224, 1189
Eco47I GGWCC 1 cut(s) 584
Eco53kI GAGCTC 2 cut(s) 118, 1150
Eco57I CTGAAG 1 cut(s) 441
Eco88I CYCGRG 2 cut(s) 327, 1355
EcoICRI GAGCTC 2 cut(s) 118, 1150
EcoNI CCTNNNNNAGG 1 cut(s) 638
EcoRII CCWGG 2 cut(s) 595, 943
EcoRV GATATC 2 cut(s) 224, 1189
EcoT14I CCWWGG 1 cut(s) 523
EcoT38I GRGCYC 2 cut(s) 120, 1152
ErhI CCWWGG 1 cut(s) 523
FaeI CATG 8 cut(s) 198, 382, 397, 527, 553, 956, 1025, 1328
FaqI GGGAC 3 cut(s) 68, 276, 1068
FatI CATG 8 cut(s) 194, 378, 393, 523, 549, 952, 1021, 1324
FauNDI CATATG 1 cut(s) 1385
FblI GTMKAC 1 cut(s) 199
Fnu4HI GCNGC 5 cut(s) 23, 78, 401, 517, 1091
FokI GGATG 1 cut(s) 258
FriOI GRGCYC 2 cut(s) 120, 1152
Fsp4HI GCNGC 5 cut(s) 23, 78, 401, 517, 1091
FspBI CTAG 4 cut(s) 866, 984, 1007, 1076
GlaI GCGC 2 cut(s) 30, 145
GluI GCNGC 5 cut(s) 23, 78, 401, 517, 1091
GsaI CCCAGC 1 cut(s) 708
GsuI CTGGAG 1 cut(s) 705
HaeII RGCGCY 1 cut(s) 147
HaeIII GGCC 1 cut(s) 633
HhaI GCGC 2 cut(s) 31, 146
Hin1II CATG 8 cut(s) 198, 382, 397, 527, 553, 956, 1025, 1328
Hin6I GCGC 2 cut(s) 29, 144
HinP1I GCGC 2 cut(s) 29, 144
HinfI GANTC 4 cut(s) 1069, 1084, 1255, 1415
HphI GGTGA 2 cut(s) 307, 632
Hpy166II GTNNAC 1 cut(s) 200
Hpy188I TCNGA 5 cut(s) 130, 941, 1019, 1250, 1312
Hpy188III TCNNGA 7 cut(s) 209, 227, 722, 813, 1081, 1267, 1319
Hpy8I GTNNAC 1 cut(s) 200
HpyAV CCTTC 4 cut(s) 111, 1198, 1286, 1339
HpyCH4III ACNGT 3 cut(s) 742, 810, 1394
HpyCH4V TGCA 5 cut(s) 22, 626, 914, 989, 1342
HpyF10VI GCNNNNNNNGC 5 cut(s) 28, 42, 248, 384, 911
HpyF3I CTNAG 4 cut(s) 32, 129, 320, 1064
Hsp92II CATG 8 cut(s) 198, 382, 397, 527, 553, 956, 1025, 1328
HspAI GCGC 2 cut(s) 29, 144
Kzo9I GATC 7 cut(s) 191, 205, 748, 1040, 1295, 1327, 1429
LmnI GCTCC 4 cut(s) 69, 101, 115, 1147
Lsp1109I GCAGC 5 cut(s) 34, 64, 387, 503, 1102
LweI GCATC 2 cut(s) 613, 1329
MaeI CTAG 4 cut(s) 866, 984, 1007, 1076
MaeIII GTNAC 1 cut(s) 557
MalI GATC 7 cut(s) 193, 207, 750, 1042, 1297, 1329, 1431
MboI GATC 7 cut(s) 191, 205, 748, 1040, 1295, 1327, 1429
MboII GAAGA 7 cut(s) 61, 64, 99, 481, 851, 1137, 1359
MflI RGATCY 2 cut(s) 205, 748
MhlI GDGCHC 4 cut(s) 38, 120, 758, 1152
MluCI AATT 5 cut(s) 162, 477, 714, 1094, 1162
MlyI GAGTC 1 cut(s) 1249
MmeI TCCRAC 1 cut(s) 964
MroXI GAANNNNTTC 2 cut(s) 690, 843
MseI TTAA 5 cut(s) 255, 440, 606, 648, 773
MslI CAYNNNNRTG 1 cut(s) 548
MspA1I CMGCKG 1 cut(s) 1046
MspR9I CCNGG 2 cut(s) 597, 945
MvaI CCWGG 2 cut(s) 597, 945
MwoI GCNNNNNNNGC 5 cut(s) 28, 42, 248, 384, 911
NcoI CCATGG 1 cut(s) 523
NdeI CATATG 1 cut(s) 1385
NdeII GATC 7 cut(s) 191, 205, 748, 1040, 1295, 1327, 1429
NlaIII CATG 8 cut(s) 198, 382, 397, 527, 553, 956, 1025, 1328
NlaIV GGNNCC 2 cut(s) 207, 586
NmeAIII GCCGAG 1 cut(s) 388
NmuCI GTSAC 1 cut(s) 557
NspI RCATGY 1 cut(s) 382
PdmI GAANNNNTTC 2 cut(s) 690, 843
PfeI GAWTC 3 cut(s) 1069, 1084, 1415
PkrI GCNGC 5 cut(s) 24, 79, 402, 518, 1092
PleI GAGTC 1 cut(s) 1249
PpsI GAGTC 1 cut(s) 1249
PsiI TTATAA 1 cut(s) 713
Psp124BI GAGCTC 2 cut(s) 120, 1152
Psp6I CCWGG 2 cut(s) 595, 943
PspFI CCCAGC 1 cut(s) 704
PspGI CCWGG 2 cut(s) 595, 943
PspN4I GGNNCC 2 cut(s) 207, 586
PspPI GGNCC 1 cut(s) 584
PsrI GAACNNNNNNTAC 2 cut(s) 1314, 1346
PstNI CAGNNNCTG 1 cut(s) 995
PsuI RGATCY 2 cut(s) 205, 748
PvuII CAGCTG 1 cut(s) 1046
RsaI GTAC 1 cut(s) 532
RsaNI GTAC 1 cut(s) 531
RseI CAYNNNNRTG 1 cut(s) 548
SacI GAGCTC 2 cut(s) 120, 1152
SaqAI TTAA 5 cut(s) 255, 440, 606, 648, 773
SatI GCNGC 5 cut(s) 23, 78, 401, 517, 1091
Sau3AI GATC 7 cut(s) 191, 205, 748, 1040, 1295, 1327, 1429
Sau96I GGNCC 1 cut(s) 584
SchI GAGTC 1 cut(s) 1249
ScrFI CCNGG 2 cut(s) 597, 945
SduI GDGCHC 4 cut(s) 38, 120, 758, 1152
SfaNI GCATC 2 cut(s) 613, 1329
SinI GGWCC 1 cut(s) 584
SmiMI CAYNNNNRTG 1 cut(s) 548
SmlI CTYRAG 2 cut(s) 87, 180
SmoI CTYRAG 2 cut(s) 87, 180
Sse9I AATT 5 cut(s) 162, 477, 714, 1094, 1162
SsiI CCGC 2 cut(s) 664, 905
SspMI CTAG 4 cut(s) 866, 984, 1007, 1076
SstI GAGCTC 2 cut(s) 120, 1152
StyD4I CCNGG 2 cut(s) 595, 943
StyI CCWWGG 1 cut(s) 523
TaaI ACNGT 3 cut(s) 742, 810, 1394
TaqI TCGA 5 cut(s) 41, 758, 1120, 1268, 1370
TasI AATT 5 cut(s) 162, 477, 714, 1094, 1162
TfiI GAWTC 3 cut(s) 1069, 1084, 1415
Tru1I TTAA 5 cut(s) 255, 440, 606, 648, 773
Tru9I TTAA 5 cut(s) 255, 440, 606, 648, 773
TscAI CASTG 1 cut(s) 418
TseFI GTSAC 1 cut(s) 557
TseI GCWGC 5 cut(s) 22, 77, 400, 516, 1090
Tsp45I GTSAC 1 cut(s) 557
TspDTI ATGAA 4 cut(s) 93, 422, 579, 801
TspGWI ACGGA 1 cut(s) 966
TspRI CASTG 1 cut(s) 418
VpaK11BI GGWCC 1 cut(s) 584
XagI CCTNNNNNAGG 1 cut(s) 638
XapI RAATTY 2 cut(s) 477, 1162
XceI RCATGY 1 cut(s) 382
XmiI GTMKAC 1 cut(s) 199
XmnI GAANNNNTTC 2 cut(s) 690, 843
XspI CTAG 4 cut(s) 866, 984, 1007, 1076
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.