MD10G1261500.v1.1

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011177
Physical Location & Seq
Reverse (-)
35566100 .. 35574151
8052 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1261500.v1.1.491

Sequence Viewer

Length: 2685 bp
ATGCAAGGAAAAAGAAAAAAAAAATTAACACACACTCTCTCTCTCTCTTCTCTTTCTCTCTCTATCTCTGTCTCGTATCGGGCTCTATCTATCTCAGCTGCTGCCTTCTCAAATCTTCGTCTCGCTTTCTCTCTCCTCACTTTCTCTCTCATCTCTTTCACTTACAATGCGCTGTTGATGAGTTTGATGTCTCACCCCAACAAACGCTCCTCAGCGATCAACCACAGCGGCACCTCCTCCTCCTCCTCCTCCTCCTCCTCTCTCAACCCTTCTTCCGCCGGCCCACCAATGAAAAAGGCCAAGTCGCAGGCGGTGGCCGGTTCCCTCGATCCCAGCAAAAATGGCCTCCACCACCATCACCACCACCACCCTCACACTCACCCGTCCCAGGACCCCGATAACGACGCCGTATTCGACCCCTCTTCCATGTCCCTCGACGAAGATCTCAAGCCCGACGACCCCTCTCCCCGAGGCATCGCCGCCAATTTGTCCCGCAAAAAGGCGCAGCCCCCTCAGCCCTCTACCAAGAAGCTTGTCATCAAGCTCGTCAAAGCTAAACCCACGTTACCCACGAATTTTGAAGAGGAAACATGGGCAAAGCTGAAATCAGCTATTTCTGCTATATTCTTAAAGAAACCTGATTCTTGTGACTTGGAGAAGCTTTATCAGGCCGTTAATGATCTCTGCCTGCACAAGATGGGAGGAAGTCTTTATCAACGAATTGAGAAGGAGTGTGAAAGGCACATAGCTGCAGCGCTGCAATCTTTGGTTGGCCAAAGCCCAGATTTAGTGGTTTTTTTATCACTCGTTGAGAGATGCTGGCAGGACCTTTGTGACCAGATGTTAATGATTCGTGGTATAGCTTTATATCTTGATAGGACATACGTGAAACAAACTCCAAATGTGCGTTCGTTGTGGGACATGGGATTGCAGCTTTTCCGCAAACACCTTTCTCTGTCTCCAGAAGTTGAGCACAAAACTGTCACCGGCCTTTTAAGAATGATCGAGAAAGAAAGATTAGGTGAAGCAGTGGCTAGGACTCTGCTTAACCATCTTTTGAAGATGTTTACAGCTTTAGGAATCTACTCAGAAAGCTTTGAGAAGCCATTTCTTGAGTGTACTTCTGAATTCTATGCTGCTGAAGGCATGAAATATATGCAGCAAGCAGATGTTCCGGATTACTTGAAGCATGTGGAGACAAGGTTGCATGAAGAACATGAAAGATGTTTAATCTACCTGGATGCAAGTACACGAAAGCCATTAGTTGCAACAGCGGAAAAACAGCTTTTAGATCGCCATATACCAGCTATTCTCGACAAGGGTTTCACATTGCTGATGGATGGGAATCGCATTGAGGACCTTCAAAGGATGCACACTCTTTTTTCCAGAGTCAATGCCCTTGAATCACTAAGGCAAGCACTTAGCTCATACATCCGGAGGACAGGGCAGGGCATTATCATGGATGAAGAGAAAGATAAAGAAATGGTTGCTTCTCTTTTGGAATTTAAGGCTTCTCTTGACACTATATGGGAAGAAAGCTTTTTCAAGAATGAAGTATTCTGCAACACCATAAAGGATGCCTTCGAGCATTTAATTAATCTCCGACAGAACCGACCTGCTGAGCTGATAGCAAAGTTTTTGGACGAGAAGCTTCGTGCTGGGAATAAGGGTACCTCTGAAGAGGAATTGGAGGGAATGCTTGACAAAGTCTTGGTTTTATTCAGGTTTATACAGGGTAAGGACGTGTTTGAAGCATTTTATAAGAAAGATCTTGCAAAAAGGCTACTTTTAGGAAAGAGTGCTTCTATTGACGCAGAGAAGTCTATGATTTCCAAGCTGAAGACTGAGTGTGGCAGTCAGTTTACAAACAAACTCGAAGGAATGTTTAAGGATATTGAACTCTCAAAAGAGATAAACGAGTCCTTCAGACAATCATCCCAGGCCAGGACAAAACTCCCATCAGGGATTGAGATGAGTGTTCATGTCCTTACAACTGGGTACTGGCCAACGTATCCTCCCATGGATGTTAGACTTCCTCATGAATTGAATGTATACCAGGATATCTTCAAGGAGTTTTACCTAAGCAAGTACAGTGGTAGGCGTTTAATGTGGCAAAATTCATTAGGTCACTGTGTCTTGAAAGCAGATTTTCCTAAAGGCAAAAAGGAGCTTGCAGTTTCTCTGTTTCAGACTGTTGTCTTGATGCTGTTTAATGACGCTGAGAAGCTAAGCCTTGAAGACATTAAGGATTCAACTGGCATTGAGGACAAGGAACTGAGAAGAACTCTGCAGTCCCTTGCATGTGGAAAAGTTCGTGTCCTACAAAAGTTTCCCAAAGGGAGAGACGTGGATGATGGTGATACATTTACGTTCAATGACACTTTCATTGCTCCTCTGTACCGCATAAAGGTGAATGCGATCCAAATGAAGGAAACAGTGGAGGAGAACACGAGCACCACTGAAAGAGTATTCCAAGACCGCCAATATCAGGTTGATGCTGCTATTGTTCGGATAATGAAAACTAGGAAGGTGCTGAGTCACACTCTTCTGATAACAGAACTGTTCCAACAGCTTAAATTTCCGATAAAGCCGGCAGATTTGAAGAAAAGGATTGAAAGCCTGATTGACCGGGAGTACCTAGAACGGGACAAGAACAACCCTCAAATTTACAACTACCTAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0000209 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006281 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009648 GO:0009719 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0009987 GO:0010033 GO:0010099 GO:0010100 GO:0010154 GO:0010182 GO:0010228 GO:0016567 GO:0019538 GO:0019899 GO:0022414 GO:0023052 GO:0031461 GO:0031625 GO:0032446 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0033554 GO:0034641 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048316 GO:0048366 GO:0048367 GO:0048519 GO:0048572 GO:0048573 GO:0048575 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048608 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0051716 GO:0061458 GO:0065007 GO:0070647 GO:0070887 GO:0071310 GO:0071322 GO:0071495 GO:0071704 GO:0080008 GO:0090304 GO:0090567 GO:0099402 GO:1901360 GO:1901564 GO:1901700 GO:1901701 GO:1902494 GO:1990234 GO:2000026 GO:2000030
KEGG Pathways
Metabolic & Signaling
ko03420 ko04120 map03420 map04120
Pfam Domains
Protein Families
Cullin Cullin_Nedd8

Protein Analysis

895

Amino Acids

101.82

Weight (kDa)

8.79

Isoelectric Point (pI)

44.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 198 - 639 5.5e-154 Cullin alpha solenoid domain
Cullin_AB PF26557 662 - 795 4.8e-48 Cullin alpha+beta domain
Cullin_Nedd8 PF10557 826 - 886 2.7e-24 Cullin protein neddylation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46210
fragaria_vesca FvH4_3g05480 FvH4_3g05480 FvH4_3g05480
malus_domestica MD05G1283200.v1.1 MD10G1261500.v1.1
prunus_persica Prupe.4G081400_v2.0.a1
pyrus_communis pycom05g26250 pycom10g21770
rosa_chinensis RchiOBHm_Chr5g0014271
rosa_laevigata RLG00000032095
rosa_multiflora Rmu_co8222354.1_g000001 Rmu_sc0009295.1_g000011
rosa_roxburghii Rroxscaffold_1G00062300
rosa_rugosa Rorug05G0016700
rosa_samantha Rh5AG111100 Rh5BG108300 Rh5CG119900 Rh5DG106900
rosa_wichuraiana Rw5G009690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1761
Acc36I ACCTGC 1 cut(s) 1624
Acc65I GGTACC 1 cut(s) 1670
AccB1I GGYRCC 2 cut(s) 230, 1670
AccI GTMKAC 1 cut(s) 2052
AccIII TCCGGA 2 cut(s) 1174, 1434
AciI CCGC 9 cut(s) 228, 276, 311, 480, 493, 940, 1274, 2401, 2479
AclWI GGATC 2 cut(s) 323, 2413
AcoI YGGCCR 3 cut(s) 315, 772, 2003
AcsI RAATTY 6 cut(s) 574, 1127, 1502, 2116, 2576, 2664
AcuI CTGAAG 4 cut(s) 1161, 1698, 1859, 1909
AcyI GRCGYC 1 cut(s) 405
AfaI GTAC 7 cut(s) 1120, 1249, 1672, 2000, 2090, 2399, 2636
AfeI AGCGCT 1 cut(s) 756
AfiI CCNNNNNNNGG 7 cut(s) 388, 499, 1944, 2407, 2428, 2488, 2644
AflIII ACRYGT 1 cut(s) 1743
AjiI CACGTC 2 cut(s) 1744, 2347
AjnI CCWGG 5 cut(s) 387, 1236, 1938, 1943, 2055
AjuI GAANNNNNNNTTGG 2 cut(s) 892, 924
Alw21I GWGCWC 2 cut(s) 975, 2456
Alw26I GTCTC 6 cut(s) 76, 125, 195, 963, 1190, 2337
AlwI GGATC 2 cut(s) 323, 2413
AlwNI CAGNNNCTG 1 cut(s) 101
Ama87I CYCGRG 1 cut(s) 468
Aor13HI TCCGGA 2 cut(s) 1174, 1434
Aor51HI AGCGCT 1 cut(s) 756
AoxI GGCC 9 cut(s) 280, 297, 315, 343, 669, 772, 988, 1941, 2003
ApoI RAATTY 6 cut(s) 574, 1127, 1502, 2116, 2576, 2664
ArsI GACNNNNNNTTYG 2 cut(s) 395, 427
AseI ATTAAT 1 cut(s) 1596
Asp718I GGTACC 1 cut(s) 1670
AspLEI GCGC 3 cut(s) 172, 505, 757
AspS9I GGNCC 4 cut(s) 281, 391, 826, 1357
AsuC2I CCSGG 1 cut(s) 2630
AsuHPI GGTGA 7 cut(s) 185, 350, 371, 976, 1034, 2369, 2422
AvaI CYCGRG 1 cut(s) 468
AvaII GGWCC 3 cut(s) 391, 826, 1357
BalI TGGCCA 2 cut(s) 774, 2005
BanI GGYRCC 2 cut(s) 230, 1670
BanII GRGCYC 1 cut(s) 85
BauI CACGAG 1 cut(s) 2449
BbsI GAAGAC 2 cut(s) 1847, 2244
Bbv12I GWGCWC 2 cut(s) 975, 2456
BbvCI CCTCAGC 2 cut(s) 211, 513
BccI CCATC 7 cut(s) 363, 691, 1059, 1330, 1334, 1966, 2348
BceAI ACGGC 2 cut(s) 392, 656
BciT130I CCWGG 5 cut(s) 389, 1238, 1940, 1945, 2057
BciVI GTATCC 1 cut(s) 2022
BcnI CCSGG 1 cut(s) 2630
BcoDI GTCTC 6 cut(s) 76, 125, 195, 963, 1190, 2337
BfaI CTAG 4 cut(s) 1035, 2523, 2639, 2678
BfmI CTRYAG 2 cut(s) 750, 2288
BfoI RGCGCY 1 cut(s) 758
BfuAI ACCTGC 1 cut(s) 1624
BfuI GTATCC 1 cut(s) 2022
BglII AGATCT 2 cut(s) 442, 1768
BlpI GCTNAGC 2 cut(s) 1620, 2228
Bme1390I CCNGG 6 cut(s) 389, 1238, 1940, 1945, 2057, 2630
Bme18I GGWCC 3 cut(s) 391, 826, 1357
BmeT110I CYCGRG 1 cut(s) 468
BmgBI CACGTC 2 cut(s) 1744, 2347
BmgT120I GGNCC 4 cut(s) 281, 391, 826, 1357
BmiI GGNNCC 4 cut(s) 232, 322, 393, 1672
BmrFI CCNGG 6 cut(s) 389, 1238, 1940, 1945, 2057, 2630
BmrI ACTGGG 1 cut(s) 2004
BmsI GCATC 7 cut(s) 483, 806, 1231, 1359, 1567, 2193, 2485
BmuI ACTGGG 1 cut(s) 2004
BoxI GACNNNNGTC 1 cut(s) 2195
BpiI GAAGAC 2 cut(s) 1847, 2244
BplI GAGNNNNNCTC 4 cut(s) 2269, 2301, 2527, 2559
BpmI CTGGAG 1 cut(s) 945
Bpu10I CCTNAGC 3 cut(s) 211, 513, 2081
Bpu1102I GCTNAGC 2 cut(s) 1620, 2228
BpuEI CTTGAG 2 cut(s) 431, 1133
BpuMI CCSGG 1 cut(s) 2630
BsaAI YACGTR 1 cut(s) 886
BsaBI GATNNNNATC 1 cut(s) 1344
BsaHI GRCGYC 1 cut(s) 405
BsaJI CCNNGG 4 cut(s) 387, 469, 1938, 2019
BsaWI WCCGGW 2 cut(s) 1174, 1434
BsaXI ACNNNNNCTCC 6 cut(s) 191, 221, 1187, 1217, 1999, 2029
Bsc4I CCNNNNNNNGG 7 cut(s) 388, 499, 1944, 2407, 2428, 2488, 2644
Bse118I RCCGGY 4 cut(s) 278, 317, 986, 2590
Bse1I ACTGG 3 cut(s) 1999, 2006, 2260
Bse3DI GCAATG 2 cut(s) 1328, 2385
Bse8I GATNNNNATC 1 cut(s) 1344
BseAI TCCGGA 2 cut(s) 1174, 1434
BseBI CCWGG 5 cut(s) 389, 1238, 1940, 1945, 2057
BseDI CCNNGG 4 cut(s) 387, 469, 1938, 2019
BseGI GGATG 9 cut(s) 1246, 1345, 1374, 1431, 1468, 1582, 1934, 2029, 2356
BseJI GATNNNNATC 1 cut(s) 1344
BseLI CCNNNNNNNGG 7 cut(s) 388, 499, 1944, 2407, 2428, 2488, 2644
BseMI GCAATG 2 cut(s) 1328, 2385
BseMII CTCAG 9 cut(s) 108, 225, 527, 1101, 1611, 1836, 2211, 2267, 2525
BseNI ACTGG 3 cut(s) 1999, 2006, 2260
BseYI CCCAGC 2 cut(s) 332, 1658
BsgI GTGCAG 1 cut(s) 674
BshFI GGCC 9 cut(s) 282, 299, 317, 345, 671, 774, 990, 1943, 2005
BshNI GGYRCC 2 cut(s) 230, 1670
BsiHKAI GWGCWC 2 cut(s) 975, 2456
BsiHKCI CYCGRG 1 cut(s) 468
BsiSI CCGG 7 cut(s) 279, 318, 987, 1175, 1435, 2591, 2629
BslFI GGGAC 6 cut(s) 370, 415, 475, 932, 2278, 2660
BslI CCNNNNNNNGG 7 cut(s) 388, 499, 1944, 2407, 2428, 2488, 2644
BsmAI GTCTC 6 cut(s) 76, 125, 195, 963, 1190, 2337
BsmBI CGTCTC 2 cut(s) 125, 2337
BsmFI GGGAC 6 cut(s) 370, 415, 475, 932, 2278, 2660
BsmI GAATGC 2 cut(s) 1701, 2419
BsnI GGCC 9 cut(s) 282, 299, 317, 345, 671, 774, 990, 1943, 2005
BsoBI CYCGRG 1 cut(s) 468
Bsp1286I GDGCHC 3 cut(s) 85, 975, 2456
Bsp13I TCCGGA 2 cut(s) 1174, 1434
Bsp143I GATC 8 cut(s) 216, 328, 442, 679, 1002, 1291, 1768, 2418
Bsp1720I GCTNAGC 2 cut(s) 1620, 2228
Bsp19I CCATGG 1 cut(s) 2019
BspACI CCGC 9 cut(s) 228, 276, 311, 480, 493, 940, 1274, 2401, 2479
BspANI GGCC 9 cut(s) 282, 299, 317, 345, 671, 774, 990, 1943, 2005
BspCNI CTCAG 9 cut(s) 107, 224, 526, 1100, 1612, 1837, 2212, 2268, 2526
BspEI TCCGGA 2 cut(s) 1174, 1434
BspHI TCATGA 1 cut(s) 2038
BspLI GGNNCC 4 cut(s) 232, 322, 393, 1672
BspMAI CTGCAG 2 cut(s) 754, 2292
BspMI ACCTGC 1 cut(s) 1624
BspPI GGATC 2 cut(s) 323, 2413
BspT107I GGYRCC 2 cut(s) 230, 1670
BsrDI GCAATG 2 cut(s) 1328, 2385
BsrFI RCCGGY 4 cut(s) 278, 317, 986, 2590
BsrI ACTGG 3 cut(s) 1999, 2006, 2260
BssAI RCCGGY 4 cut(s) 278, 317, 986, 2590
BssECI CCNNGG 4 cut(s) 387, 469, 1938, 2019
BssMI GATC 8 cut(s) 216, 328, 442, 679, 1002, 1291, 1768, 2418
BssNAI GTATAC 1 cut(s) 2053
BssNI GRCGYC 1 cut(s) 405
BssSI CACGAG 1 cut(s) 2449
BssT1I CCWWGG 1 cut(s) 2019
Bst1107I GTATAC 1 cut(s) 2053
Bst2BI CACGAG 1 cut(s) 2449
Bst2UI CCWGG 5 cut(s) 389, 1238, 1940, 1945, 2057
Bst4CI ACNGT 6 cut(s) 982, 2093, 2132, 2194, 2437, 2562
Bst6I CTCTTC 6 cut(s) 52, 427, 576, 1461, 1674, 2550
BstACI GRCGYC 1 cut(s) 405
BstBAI YACGTR 1 cut(s) 886
BstC8I GCNNGC 8 cut(s) 280, 309, 689, 821, 1164, 1416, 2172, 2592
BstDSI CCRYGG 1 cut(s) 2019
BstF5I GGATG 9 cut(s) 1246, 1345, 1374, 1431, 1468, 1582, 1934, 2029, 2356
BstH2I RGCGCY 1 cut(s) 758
BstHHI GCGC 3 cut(s) 172, 505, 757
BstKTI GATC 8 cut(s) 219, 331, 445, 682, 1005, 1294, 1771, 2421
BstMAI GTCTC 6 cut(s) 76, 125, 195, 963, 1190, 2337
BstMBI GATC 8 cut(s) 216, 328, 442, 679, 1002, 1291, 1768, 2418
BstMWI GCNNNNNNNGC 3 cut(s) 342, 514, 617
BstNI CCWGG 5 cut(s) 389, 1238, 1940, 1945, 2057
BstNSI RCATGY 2 cut(s) 1193, 2304
BstPAI GACNNNNGTC 1 cut(s) 2195
BstSCI CCNGG 6 cut(s) 387, 1236, 1938, 1943, 2055, 2628
BstSFI CTRYAG 2 cut(s) 750, 2288
BstV2I GAAGAC 2 cut(s) 1847, 2244
BstX2I RGATCY 2 cut(s) 442, 1768
BstYI RGATCY 2 cut(s) 442, 1768
BstZ17I GTATAC 1 cut(s) 2053
BsuI GTATCC 1 cut(s) 2022
BsuRI GGCC 9 cut(s) 282, 299, 317, 345, 671, 774, 990, 1943, 2005
BtgI CCRYGG 1 cut(s) 2019
BtgZI GCGATG 1 cut(s) 460
BtrI CACGTC 2 cut(s) 1744, 2347
BtsCI GGATG 9 cut(s) 1246, 1345, 1374, 1431, 1468, 1582, 1934, 2029, 2356
BtsI GCAGTG 1 cut(s) 1035
BtsIMutI CAGTG 5 cut(s) 1035, 2098, 2128, 2442, 2457
BveI ACCTGC 1 cut(s) 1624
Cac8I GCNNGC 8 cut(s) 280, 309, 689, 821, 1164, 1416, 2172, 2592
CaiI CAGNNNCTG 1 cut(s) 101
CciI TCATGA 1 cut(s) 2038
CfoI GCGC 3 cut(s) 172, 505, 757
Cfr10I RCCGGY 4 cut(s) 278, 317, 986, 2590
Cfr13I GGNCC 4 cut(s) 281, 391, 826, 1357
CseI GACGC 3 cut(s) 413, 1820, 2225
Csp6I GTAC 7 cut(s) 1119, 1248, 1671, 1999, 2089, 2398, 2635
CspCI CAANNNNNGTGG 4 cut(s) 559, 594, 2074, 2109
CviQI GTAC 7 cut(s) 1119, 1248, 1671, 1999, 2089, 2398, 2635
DpnI GATC 8 cut(s) 218, 330, 444, 681, 1004, 1293, 1770, 2420
DpnII GATC 8 cut(s) 216, 328, 442, 679, 1002, 1291, 1768, 2418
EaeI YGGCCR 3 cut(s) 315, 772, 2003
Eam1104I CTCTTC 6 cut(s) 52, 427, 576, 1461, 1674, 2550
EarI CTCTTC 6 cut(s) 52, 427, 576, 1461, 1674, 2550
EciI GGCGGA 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 2019
Eco24I GRGCYC 1 cut(s) 85
Eco32I GATATC 1 cut(s) 2062
Eco47I GGWCC 3 cut(s) 391, 826, 1357
Eco47III AGCGCT 1 cut(s) 756
Eco57I CTGAAG 4 cut(s) 1161, 1698, 1859, 1909
Eco88I CYCGRG 1 cut(s) 468
EcoO109I RGGNCCY 3 cut(s) 391, 826, 1357
EcoRI GAATTC 1 cut(s) 1127
EcoRII CCWGG 5 cut(s) 387, 1236, 1938, 1943, 2055
EcoRV GATATC 1 cut(s) 2062
EcoT14I CCWWGG 1 cut(s) 2019
EcoT38I GRGCYC 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 2019
Esp3I CGTCTC 2 cut(s) 125, 2337
FalI AAGNNNNNCTT 2 cut(s) 1565, 1597
FaqI GGGAC 6 cut(s) 370, 415, 475, 932, 2278, 2660
FauI CCCGC 1 cut(s) 500
FblI GTMKAC 1 cut(s) 2052
FokI GGATG 9 cut(s) 1253, 1352, 1381, 1418, 1475, 1589, 1921, 2036, 2363
FriOI GRGCYC 1 cut(s) 85
FspBI CTAG 4 cut(s) 1035, 2523, 2639, 2678
GlaI GCGC 3 cut(s) 171, 504, 756
GsaI CCCAGC 2 cut(s) 336, 1662
GsuI CTGGAG 1 cut(s) 945
HaeII RGCGCY 1 cut(s) 758
HaeIII GGCC 9 cut(s) 282, 299, 317, 345, 671, 774, 990, 1943, 2005
HapII CCGG 7 cut(s) 279, 318, 987, 1175, 1435, 2591, 2629
HgaI GACGC 3 cut(s) 413, 1820, 2225
HhaI GCGC 3 cut(s) 172, 505, 757
Hin1I GRCGYC 1 cut(s) 405
Hin6I GCGC 3 cut(s) 170, 503, 755
HinP1I GCGC 3 cut(s) 170, 503, 755
HindIII AAGCTT 5 cut(s) 530, 659, 1093, 1537, 1649
HpaII CCGG 7 cut(s) 279, 318, 987, 1175, 1435, 2591, 2629
HphI GGTGA 7 cut(s) 185, 350, 371, 976, 1034, 2369, 2422
Hpy166II GTNNAC 5 cut(s) 1068, 1119, 1250, 1863, 2053
Hpy188I TCNGA 9 cut(s) 1090, 1126, 1604, 1678, 1928, 2190, 2511, 2550, 2583
Hpy8I GTNNAC 5 cut(s) 1068, 1119, 1250, 1863, 2053
Hpy99I CGWCG 3 cut(s) 407, 440, 458
HpyCH4III ACNGT 6 cut(s) 982, 2093, 2132, 2194, 2437, 2562
HpyCH4IV ACGT 6 cut(s) 563, 885, 1743, 2009, 2346, 2369
HpyF10VI GCNNNNNNNGC 3 cut(s) 342, 514, 617
HpySE526I ACGT 6 cut(s) 563, 885, 1743, 2009, 2346, 2369
Hsp92I GRCGYC 1 cut(s) 405
HspAI GCGC 3 cut(s) 170, 503, 755
Kpn2I TCCGGA 2 cut(s) 1174, 1434
KpnI GGTACC 1 cut(s) 1674
KroI GCCGGC 2 cut(s) 278, 2590
KroNI GCCGGC 2 cut(s) 280, 2592
Kzo9I GATC 8 cut(s) 216, 328, 442, 679, 1002, 1291, 1768, 2418
LmnI GCTCC 3 cut(s) 212, 2167, 2395
LweI GCATC 7 cut(s) 483, 806, 1231, 1359, 1567, 2193, 2485
MaeI CTAG 4 cut(s) 1035, 2523, 2639, 2678
MaeII ACGT 6 cut(s) 563, 885, 1743, 2009, 2346, 2369
MaeIII GTNAC 6 cut(s) 564, 647, 833, 982, 2126, 2537
MalI GATC 8 cut(s) 218, 330, 444, 681, 1004, 1293, 1770, 2420
MboI GATC 8 cut(s) 216, 328, 442, 679, 1002, 1291, 1768, 2418
MflI RGATCY 2 cut(s) 442, 1768
MhlI GDGCHC 3 cut(s) 85, 975, 2456
MlsI TGGCCA 2 cut(s) 774, 2005
MluNI TGGCCA 2 cut(s) 774, 2005
MlyI GAGTC 4 cut(s) 1033, 1398, 1928, 2545
MmeI TCCRAC 2 cut(s) 1627, 2590
Mox20I TGGCCA 2 cut(s) 774, 2005
MroI TCCGGA 2 cut(s) 1174, 1434
MroNI GCCGGC 2 cut(s) 278, 2590
MscI TGGCCA 2 cut(s) 774, 2005
MslI CAYNNNNRTG 2 cut(s) 1457, 2408
Msp20I TGGCCA 2 cut(s) 774, 2005
MspA1I CMGCKG 3 cut(s) 98, 228, 1274
MspI CCGG 7 cut(s) 279, 318, 987, 1175, 1435, 2591, 2629
MspR9I CCNGG 6 cut(s) 389, 1238, 1940, 1945, 2057, 2630
Mva1269I GAATGC 2 cut(s) 1701, 2419
MvaI CCWGG 5 cut(s) 389, 1238, 1940, 1945, 2057
MwoI GCNNNNNNNGC 3 cut(s) 342, 514, 617
NaeI GCCGGC 2 cut(s) 280, 2592
NciI CCSGG 1 cut(s) 2630
NcoI CCATGG 1 cut(s) 2019
NdeII GATC 8 cut(s) 216, 328, 442, 679, 1002, 1291, 1768, 2418
NgoMIV GCCGGC 2 cut(s) 278, 2590
NlaIV GGNNCC 4 cut(s) 232, 322, 393, 1672
NmuCI GTSAC 5 cut(s) 647, 833, 982, 2126, 2537
NspI RCATGY 2 cut(s) 1193, 2304
PacI TTAATTAA 1 cut(s) 1596
PagI TCATGA 1 cut(s) 2038
PcsI WCGNNNNNNNCGW 2 cut(s) 411, 569
PctI GAATGC 2 cut(s) 1701, 2419
PdiI GCCGGC 2 cut(s) 280, 2592
PfeI GAWTC 6 cut(s) 641, 850, 1080, 1345, 1403, 2249
PflFI GACNNNGTC 1 cut(s) 1706
PleI GAGTC 4 cut(s) 1033, 1397, 1927, 2544
PpsI GAGTC 4 cut(s) 1033, 1397, 1927, 2544
Ppu21I YACGTR 1 cut(s) 886
PpuMI RGGWCCY 3 cut(s) 391, 826, 1357
PshAI GACNNNNGTC 1 cut(s) 2195
PshBI ATTAAT 1 cut(s) 1596
PsiI TTATAA 1 cut(s) 1761
Psp5II RGGWCCY 3 cut(s) 391, 826, 1357
Psp6I CCWGG 5 cut(s) 387, 1236, 1938, 1943, 2055
PspFI CCCAGC 2 cut(s) 332, 1658
PspGI CCWGG 5 cut(s) 387, 1236, 1938, 1943, 2055
PspN4I GGNNCC 4 cut(s) 232, 322, 393, 1672
PspPI GGNCC 4 cut(s) 281, 391, 826, 1357
PspPPI RGGWCCY 3 cut(s) 391, 826, 1357
PstI CTGCAG 2 cut(s) 754, 2292
PstNI CAGNNNCTG 1 cut(s) 101
PsuI RGATCY 2 cut(s) 442, 1768
PsyI GACNNNGTC 1 cut(s) 1706
PvuII CAGCTG 1 cut(s) 98
RsaI GTAC 7 cut(s) 1120, 1249, 1672, 2000, 2090, 2399, 2636
RsaNI GTAC 7 cut(s) 1119, 1248, 1671, 1999, 2089, 2398, 2635
RseI CAYNNNNRTG 2 cut(s) 1457, 2408
Sau3AI GATC 8 cut(s) 216, 328, 442, 679, 1002, 1291, 1768, 2418
Sau96I GGNCC 4 cut(s) 281, 391, 826, 1357
SchI GAGTC 4 cut(s) 1033, 1398, 1928, 2545
ScrFI CCNGG 6 cut(s) 389, 1238, 1940, 1945, 2057, 2630
SduI GDGCHC 3 cut(s) 85, 975, 2456
SfaNI GCATC 7 cut(s) 483, 806, 1231, 1359, 1567, 2193, 2485
SfcI CTRYAG 2 cut(s) 750, 2288
SinI GGWCC 3 cut(s) 391, 826, 1357
SmiMI CAYNNNNRTG 2 cut(s) 1457, 2408
SmlI CTYRAG 2 cut(s) 446, 1112
SmoI CTYRAG 2 cut(s) 446, 1112
SsiI CCGC 9 cut(s) 228, 276, 311, 480, 493, 940, 1274, 2401, 2479
SspMI CTAG 4 cut(s) 1035, 2523, 2639, 2678
StyD4I CCNGG 6 cut(s) 387, 1236, 1938, 1943, 2055, 2628
StyI CCWWGG 1 cut(s) 2019
TaaI ACNGT 6 cut(s) 982, 2093, 2132, 2194, 2437, 2562
TaiI ACGT 6 cut(s) 566, 888, 1746, 2012, 2349, 2372
TaqI TCGA 7 cut(s) 327, 414, 435, 1005, 1314, 1584, 1875
TatI WGTACW 3 cut(s) 1118, 1247, 2088
TauI GCSGC 2 cut(s) 231, 482
TfiI GAWTC 6 cut(s) 641, 850, 1080, 1345, 1403, 2249
TscAI CASTG 5 cut(s) 1035, 2098, 2135, 2442, 2464
TseFI GTSAC 5 cut(s) 647, 833, 982, 2126, 2537
Tsp45I GTSAC 5 cut(s) 647, 833, 982, 2126, 2537
TspRI CASTG 5 cut(s) 1035, 2098, 2135, 2442, 2464
Tth111I GACNNNGTC 1 cut(s) 1706
VpaK11BI GGWCC 3 cut(s) 391, 826, 1357
VspI ATTAAT 1 cut(s) 1596
XapI RAATTY 6 cut(s) 574, 1127, 1502, 2116, 2576, 2664
XceI RCATGY 2 cut(s) 1193, 2304
XmiI GTMKAC 1 cut(s) 2052
XspI CTAG 4 cut(s) 1035, 2523, 2639, 2678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.