pycom05g26250

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011177
Physical Location & Seq
Reverse (-)
27550175 .. 27557819
7645 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g26250.5

Sequence Viewer

Length: 2505 bp
ATGAGTTTGATGTCTCACCCCACCAAACGCTCCTCAGCCATCAACCACAGCAGCAGCGGCACCTCCTCCTCCTCTTCCTCCTCTCTCAACCCTTCTTCCGCCGGCCCACCCATGAAAAAGGCCAAGTCGCAGGCGGTGGCCTGTTCCCTCGATCCCAGCAAAAATGGCCTCCACCACCACCACCACCACCCTCACACTCACCCTCCCCAGGACCCCGATAACGACGCCGTATTCGACCCCTCTTCCATGTCCCTCGACGATGATCTCAGGCCCGACGACCCCTCTCCCCGAGGCGTCGCCGCCAATTTGTCCCGCAAAAAGGCCCAGCCCCCTCAGCCCTCTACCAAGAAGCTCGTCATCAAGCTCGTCAAAGCTAAACCCACATTACCCACAAATTTTGAAGAGGAAACATGGGCAAAGCTGAAGTCGGCTATTTGTGCTATATTCTTGAAGAAACCTGATTCTTGTGACTTGGAGAAGCTTTATCAGGCTGTTACTGATCTCTGCCTGCACAAGATGGGAGGAAGTCTTTATCAACGGATTGAGAAGGAGTGTGAAAGACACATAGCTGCAGCGCTGCAATCTTTGGTTGGCCAAAGCCCAGATTTAGTGGTTTTTCTATCACTTGTTGAGAGATGCTGGCAGGACCTCTGGGATCAGATGTTAATGATTCGCAGTATAGCCTTATATCTTGATAGGACATATGTGAAACAAACACCAAATGTGCGTTCATTGTGGGACATGGGATTGCAGCTTTTCCGCAAACATCTTTCTCTGTCTCCAGAAGTTGAACACAAAACTGTCACTGGCCTTTTAAGAATGATAGAGAAAGAAAGATTAGGTGAAGCAGTGGCCAGGACTCTGCTTAACCATCTTTTGAAGATGTTTACAGCTTTAGGGATCTACTCAGAAAGCTTTGAGAAGCCATTTCTTGAATGTACTTCTGAATTCTATGCTGCTGTAGGCATGAAATATATGCAGCAAGCAGATGTTCCGGATTATTTGAAGCATGTGGAGACAAGGTTGCATGAAGAACATGAAAGATGTTTAATCTACCTGGATGCAAGTACAAGAAAGCCATTAGTTGCAACAGCGGAAAAACAGCTTTTAGAGCGCCATATTCCTGCCATTCTCGACAAGGGTTTCACATTGCTGATGGATGGGAATCGCATTGAGGACCTTCAAAGGATGCACACTCTTTTCTCCAGAGTCAATGCCCTTGAATCACTAAGGCAAGCGCTTAGCTCATACATCCGGAGGACAGGGCAGGGGATTATCATGGATGAAGAAAAAGATAAAGAAATGGTTGCTTCTCTTTTGGAATTTAAGGCTTCTCTTGACACTATATGGGAAGAAAGCTTTTTCAAGAATGAAGTATTTTGCAACACCATAAAGGATGCCTTCGAGCATTTAATTAATCTCCGACAGAACCGACCTGCTGAGCTGATTGCCAAGTTTTTGGACGAGAAGCTTCGTGCTGGGAATAAGGGTACTTCTGAAGAGGAATTGGAGGGAATGCTTGATAAGGTCTTGGTTTTATTCAGGTTTATACAGGGTAAGGACGTGTTTGAAGCATTTTATAAGAAAGATCTTGCAAAAAGGCTACTGTTGGGAAAGAGTGCTTCTATTGACGCAGAGAAGTCTATGATTTCCAAGCTGAAGACCGAGTGTGGCAGTCAGTTTACAAACAAACTCGAAGGAATGTTCAAGGATATTGAACTGTCGAAAGAGATAAACGAGTCCTTCAAACAATCATCCCAGGCTAGGACAAAACTCCCATCAGGGATTGAGATGAGTGTTCATGTCCTTACGACTGGGTACTGGCCTACATATCCTCCCATGGATGTTAGACTTCCTCATGAATTGAATGTATACCAGGATATCTTCAAGGAGTTTTACCTAAGCAAGTACAGTGGTAGGCGTTTGATGTGGCAAAATTCATTAGGCCACTGTGTCTTGAAAGCAGAGTTTCCTAAAGGCAAAAAGGAGCTTGCAGTTTCTCTGTTTCAGACCGTTGTCTTGATGCTGTTTAATGATGCTGAGAAGCTAAGCCTTGAAGACATTAAGGATTCAACTGGCATTGAGGACAAGGAACTGAGAAGAACTCTGCAGTCCCTTGCATGTGGAAAAGTTCGTGTCCTACAAAAGTTTCCCAAAGGGAGAGAAGTGGACGATGGCGATACATTTACGTTCAATGATTCATTTACTGCTCCTCTCTACCGCATAAAGGTGAATGCGATCCAAATGAAGGAAACAGTGGAGGAGAACACGAGCACCACTGAAAGAGTATTTCAAGACCGCCAATATCAGGTTGATGCTGCAATTGTTCGGATAATGAAAACTAGGAAGGTGCTGAGTCACACTCTTCTGATAACAGAACTGTTCCAACAGCTTAAATTTCCGATTAAGCCGGCAGATTTGAAGAAAAGGATTGAAAGCCTGATTGACCGGGAGTACCTAGAACGGGACAAGAACAACCCGCAAATTTACAACTACCTAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0000209 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006281 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009648 GO:0009719 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0009987 GO:0010033 GO:0010099 GO:0010100 GO:0010154 GO:0010182 GO:0010228 GO:0016567 GO:0019538 GO:0019899 GO:0022414 GO:0023052 GO:0031461 GO:0031625 GO:0032446 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0033554 GO:0034641 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048316 GO:0048366 GO:0048367 GO:0048519 GO:0048572 GO:0048573 GO:0048575 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048608 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0051716 GO:0061458 GO:0065007 GO:0070647 GO:0070887 GO:0071310 GO:0071322 GO:0071495 GO:0071704 GO:0080008 GO:0090304 GO:0090567 GO:0099402 GO:1901360 GO:1901564 GO:1901700 GO:1901701 GO:1902494 GO:1990234 GO:2000026 GO:2000030
KEGG Pathways
Metabolic & Signaling
ko03420 ko04120 map03420 map04120
Pfam Domains
Protein Families
Cullin Cullin_Nedd8

Protein Analysis

835

Amino Acids

95.35

Weight (kDa)

8.34

Isoelectric Point (pI)

48.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46210
fragaria_vesca FvH4_3g05480 FvH4_3g05480 FvH4_3g05480
malus_domestica MD05G1283200.v1.1 MD10G1261500.v1.1
prunus_persica Prupe.4G081400_v2.0.a1
pyrus_communis pycom05g26250 pycom10g21770
rosa_chinensis RchiOBHm_Chr5g0014271
rosa_laevigata RLG00000032095
rosa_multiflora Rmu_co8222354.1_g000001 Rmu_sc0009295.1_g000011
rosa_roxburghii Rroxscaffold_1G00062300
rosa_rugosa Rorug05G0016700
rosa_samantha Rh5AG111100 Rh5BG108300 Rh5CG119900 Rh5DG106900
rosa_wichuraiana Rw5G009690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1581
Acc36I ACCTGC 1 cut(s) 1444
AccB1I GGYRCC 1 cut(s) 59
AccI GTMKAC 1 cut(s) 1872
AccIII TCCGGA 2 cut(s) 994, 1254
AclWI GGATC 4 cut(s) 146, 663, 908, 2233
AcoI YGGCCR 2 cut(s) 592, 852
AcsI RAATTY 6 cut(s) 394, 947, 1322, 1936, 2396, 2484
AcuI CTGAAG 3 cut(s) 443, 1518, 1679
AcyI GRCGYC 2 cut(s) 225, 294
AfaI GTAC 6 cut(s) 940, 1069, 1492, 1820, 1910, 2456
AfeI AGCGCT 2 cut(s) 576, 1239
AfiI CCNNNNNNNGG 7 cut(s) 208, 319, 1764, 2227, 2248, 2308, 2464
AflIII ACRYGT 1 cut(s) 1563
AjiI CACGTC 1 cut(s) 1564
AjnI CCWGG 5 cut(s) 207, 854, 1056, 1758, 1875
AjuI GAANNNNNNNTTGG 2 cut(s) 712, 744
Alw21I GWGCWC 1 cut(s) 2276
Alw26I GTCTC 3 cut(s) 18, 783, 1010
AlwI GGATC 4 cut(s) 146, 663, 908, 2233
Ama87I CYCGRG 1 cut(s) 288
Aor13HI TCCGGA 2 cut(s) 994, 1254
Aor51HI AGCGCT 2 cut(s) 576, 1239
ApeKI GCWGC 9 cut(s) 51, 54, 569, 572, 577, 751, 956, 979, 2318
ApoI RAATTY 6 cut(s) 394, 947, 1322, 1936, 2396, 2484
ArsI GACNNNNNNTTYG 4 cut(s) 215, 247, 410, 442
AseI ATTAAT 1 cut(s) 1416
AspLEI GCGC 3 cut(s) 577, 1116, 1240
AspS9I GGNCC 6 cut(s) 104, 211, 270, 322, 646, 1177
AsuC2I CCSGG 1 cut(s) 2450
AsuHPI GGTGA 4 cut(s) 8, 191, 854, 2242
AvaI CYCGRG 1 cut(s) 288
AvaII GGWCC 3 cut(s) 211, 646, 1177
BalI TGGCCA 2 cut(s) 594, 854
BanI GGYRCC 1 cut(s) 59
BauI CACGAG 1 cut(s) 2269
BbsI GAAGAC 2 cut(s) 1667, 2064
Bbv12I GWGCWC 1 cut(s) 2276
BbvCI CCTCAGC 2 cut(s) 34, 333
BbvI GCAGC 9 cut(s) 63, 66, 556, 564, 584, 763, 943, 991, 2305
BccI CCATC 7 cut(s) 47, 511, 879, 1150, 1154, 1786, 2168
BceAI ACGGC 1 cut(s) 212
BciT130I CCWGG 5 cut(s) 209, 856, 1058, 1760, 1877
BcnI CCSGG 1 cut(s) 2450
BcoDI GTCTC 3 cut(s) 18, 783, 1010
BfaI CTAG 4 cut(s) 1764, 2343, 2459, 2498
BfmI CTRYAG 3 cut(s) 570, 960, 2108
BfoI RGCGCY 3 cut(s) 578, 1117, 1241
BfuAI ACCTGC 1 cut(s) 1444
BglII AGATCT 1 cut(s) 1588
BlpI GCTNAGC 3 cut(s) 1241, 1440, 2048
Bme1390I CCNGG 6 cut(s) 209, 856, 1058, 1760, 1877, 2450
Bme18I GGWCC 3 cut(s) 211, 646, 1177
BmeT110I CYCGRG 1 cut(s) 288
BmgBI CACGTC 1 cut(s) 1564
BmgT120I GGNCC 6 cut(s) 104, 211, 270, 322, 646, 1177
BmiI GGNNCC 2 cut(s) 61, 213
BmrFI CCNGG 6 cut(s) 209, 856, 1058, 1760, 1877, 2450
BmrI ACTGGG 1 cut(s) 1824
BmsI GCATC 7 cut(s) 626, 1051, 1179, 1387, 2013, 2026, 2305
BmuI ACTGGG 1 cut(s) 1824
BoxI GACNNNNGTC 1 cut(s) 2015
BpiI GAAGAC 2 cut(s) 1667, 2064
BplI GAGNNNNNCTC 4 cut(s) 2089, 2121, 2347, 2379
BpmI CTGGAG 2 cut(s) 765, 1189
Bpu10I CCTNAGC 3 cut(s) 34, 333, 1901
Bpu1102I GCTNAGC 3 cut(s) 1241, 1440, 2048
BpuMI CCSGG 1 cut(s) 2450
BsaBI GATNNNNATC 1 cut(s) 1164
BsaHI GRCGYC 2 cut(s) 225, 294
BsaJI CCNNGG 4 cut(s) 207, 289, 1758, 1839
BsaWI WCCGGW 2 cut(s) 994, 1254
BsaXI ACNNNNNCTCC 6 cut(s) 187, 217, 1007, 1037, 1819, 1849
Bsc4I CCNNNNNNNGG 7 cut(s) 208, 319, 1764, 2227, 2248, 2308, 2464
Bse118I RCCGGY 2 cut(s) 101, 2410
Bse1I ACTGG 4 cut(s) 811, 1819, 1826, 2080
Bse3DI GCAATG 1 cut(s) 1148
Bse8I GATNNNNATC 1 cut(s) 1164
BseAI TCCGGA 2 cut(s) 994, 1254
BseBI CCWGG 5 cut(s) 209, 856, 1058, 1760, 1877
BseDI CCNNGG 4 cut(s) 207, 289, 1758, 1839
BseGI GGATG 8 cut(s) 1066, 1165, 1194, 1251, 1288, 1402, 1754, 1849
BseJI GATNNNNATC 1 cut(s) 1164
BseLI CCNNNNNNNGG 7 cut(s) 208, 319, 1764, 2227, 2248, 2308, 2464
BseMI GCAATG 1 cut(s) 1148
BseMII CTCAG 8 cut(s) 48, 280, 347, 921, 1431, 2031, 2087, 2345
BseNI ACTGG 4 cut(s) 811, 1819, 1826, 2080
BseRI GAGGAG 7 cut(s) 22, 55, 58, 61, 70, 2202, 2276
BseXI GCAGC 9 cut(s) 63, 66, 556, 564, 584, 763, 943, 991, 2305
BseYI CCCAGC 3 cut(s) 155, 324, 1478
BsgI GTGCAG 1 cut(s) 494
BshNI GGYRCC 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 2276
BsiHKCI CYCGRG 1 cut(s) 288
BsiSI CCGG 5 cut(s) 102, 995, 1255, 2411, 2449
BslFI GGGAC 5 cut(s) 235, 295, 752, 2098, 2480
BslI CCNNNNNNNGG 7 cut(s) 208, 319, 1764, 2227, 2248, 2308, 2464
BsmAI GTCTC 3 cut(s) 18, 783, 1010
BsmFI GGGAC 5 cut(s) 235, 295, 752, 2098, 2480
BsmI GAATGC 2 cut(s) 1521, 2239
BsoBI CYCGRG 1 cut(s) 288
Bsp1286I GDGCHC 1 cut(s) 2276
Bsp13I TCCGGA 2 cut(s) 994, 1254
Bsp143I GATC 7 cut(s) 151, 262, 499, 655, 900, 1588, 2238
Bsp1720I GCTNAGC 3 cut(s) 1241, 1440, 2048
Bsp19I CCATGG 1 cut(s) 1839
BspCNI CTCAG 8 cut(s) 47, 279, 346, 920, 1432, 2032, 2088, 2346
BspEI TCCGGA 2 cut(s) 994, 1254
BspHI TCATGA 1 cut(s) 1858
BspLI GGNNCC 2 cut(s) 61, 213
BspMAI CTGCAG 2 cut(s) 574, 2112
BspMI ACCTGC 1 cut(s) 1444
BspPI GGATC 4 cut(s) 146, 663, 908, 2233
BspT107I GGYRCC 1 cut(s) 59
BsrDI GCAATG 1 cut(s) 1148
BsrFI RCCGGY 2 cut(s) 101, 2410
BsrI ACTGG 4 cut(s) 811, 1819, 1826, 2080
BssAI RCCGGY 2 cut(s) 101, 2410
BssECI CCNNGG 4 cut(s) 207, 289, 1758, 1839
BssMI GATC 7 cut(s) 151, 262, 499, 655, 900, 1588, 2238
BssNAI GTATAC 1 cut(s) 1873
BssNI GRCGYC 2 cut(s) 225, 294
BssSI CACGAG 1 cut(s) 2269
BssT1I CCWWGG 1 cut(s) 1839
Bst1107I GTATAC 1 cut(s) 1873
Bst2BI CACGAG 1 cut(s) 2269
Bst2UI CCWGG 5 cut(s) 209, 856, 1058, 1760, 1877
Bst4CI ACNGT 8 cut(s) 802, 1608, 1722, 1913, 1952, 2014, 2257, 2382
Bst6I CTCTTC 5 cut(s) 79, 247, 396, 1494, 2370
BstACI GRCGYC 2 cut(s) 225, 294
BstC8I GCNNGC 8 cut(s) 103, 132, 509, 641, 984, 1236, 1992, 2412
BstDSI CCRYGG 1 cut(s) 1839
BstF5I GGATG 8 cut(s) 1066, 1165, 1194, 1251, 1288, 1402, 1754, 1849
BstH2I RGCGCY 3 cut(s) 578, 1117, 1241
BstHHI GCGC 3 cut(s) 577, 1116, 1240
BstKTI GATC 7 cut(s) 154, 265, 502, 658, 903, 1591, 2241
BstMAI GTCTC 3 cut(s) 18, 783, 1010
BstMBI GATC 7 cut(s) 151, 262, 499, 655, 900, 1588, 2238
BstMWI GCNNNNNNNGC 5 cut(s) 57, 165, 334, 437, 1111
BstNI CCWGG 5 cut(s) 209, 856, 1058, 1760, 1877
BstNSI RCATGY 2 cut(s) 1013, 2124
BstPAI GACNNNNGTC 1 cut(s) 2015
BstSCI CCNGG 6 cut(s) 207, 854, 1056, 1758, 1875, 2448
BstSFI CTRYAG 3 cut(s) 570, 960, 2108
BstV1I GCAGC 9 cut(s) 63, 66, 556, 564, 584, 763, 943, 991, 2305
BstV2I GAAGAC 2 cut(s) 1667, 2064
BstX2I RGATCY 2 cut(s) 900, 1588
BstXI CCANNNNNNTGG 1 cut(s) 1459
BstYI RGATCY 2 cut(s) 900, 1588
BstZ17I GTATAC 1 cut(s) 1873
BtgI CCRYGG 1 cut(s) 1839
BtrI CACGTC 1 cut(s) 1564
BtsCI GGATG 8 cut(s) 1066, 1165, 1194, 1251, 1288, 1402, 1754, 1849
BtsI GCAGTG 1 cut(s) 855
BtsIMutI CAGTG 6 cut(s) 804, 855, 1918, 1948, 2262, 2277
BveI ACCTGC 1 cut(s) 1444
Cac8I GCNNGC 8 cut(s) 103, 132, 509, 641, 984, 1236, 1992, 2412
CciI TCATGA 1 cut(s) 1858
CfoI GCGC 3 cut(s) 577, 1116, 1240
Cfr10I RCCGGY 2 cut(s) 101, 2410
Cfr13I GGNCC 6 cut(s) 104, 211, 270, 322, 646, 1177
CseI GACGC 3 cut(s) 233, 283, 1640
Csp6I GTAC 6 cut(s) 939, 1068, 1491, 1819, 1909, 2455
CspCI CAANNNNNGTGG 4 cut(s) 379, 414, 1894, 1929
CviQI GTAC 6 cut(s) 939, 1068, 1491, 1819, 1909, 2455
DpnI GATC 7 cut(s) 153, 264, 501, 657, 902, 1590, 2240
DpnII GATC 7 cut(s) 151, 262, 499, 655, 900, 1588, 2238
EaeI YGGCCR 2 cut(s) 592, 852
Eam1104I CTCTTC 5 cut(s) 79, 247, 396, 1494, 2370
EarI CTCTTC 5 cut(s) 79, 247, 396, 1494, 2370
EciI GGCGGA 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 1839
Eco32I GATATC 1 cut(s) 1882
Eco47I GGWCC 3 cut(s) 211, 646, 1177
Eco47III AGCGCT 2 cut(s) 576, 1239
Eco57I CTGAAG 3 cut(s) 443, 1518, 1679
Eco88I CYCGRG 1 cut(s) 288
EcoO109I RGGNCCY 3 cut(s) 211, 646, 1177
EcoRI GAATTC 1 cut(s) 947
EcoRII CCWGG 5 cut(s) 207, 854, 1056, 1758, 1875
EcoRV GATATC 1 cut(s) 1882
EcoT14I CCWWGG 1 cut(s) 1839
ErhI CCWWGG 1 cut(s) 1839
FalI AAGNNNNNCTT 2 cut(s) 1385, 1417
FaqI GGGAC 5 cut(s) 235, 295, 752, 2098, 2480
FauI CCCGC 2 cut(s) 320, 2487
FauNDI CATATG 1 cut(s) 703
FblI GTMKAC 1 cut(s) 1872
FokI GGATG 8 cut(s) 1073, 1172, 1201, 1238, 1295, 1409, 1741, 1856
FspBI CTAG 4 cut(s) 1764, 2343, 2459, 2498
GlaI GCGC 3 cut(s) 576, 1115, 1239
GsaI CCCAGC 3 cut(s) 159, 328, 1482
GsuI CTGGAG 2 cut(s) 765, 1189
HaeII RGCGCY 3 cut(s) 578, 1117, 1241
HapII CCGG 5 cut(s) 102, 995, 1255, 2411, 2449
HgaI GACGC 3 cut(s) 233, 283, 1640
HhaI GCGC 3 cut(s) 577, 1116, 1240
Hin1I GRCGYC 2 cut(s) 225, 294
Hin6I GCGC 3 cut(s) 575, 1114, 1238
HinP1I GCGC 3 cut(s) 575, 1114, 1238
HindIII AAGCTT 4 cut(s) 479, 913, 1357, 1469
HpaII CCGG 5 cut(s) 102, 995, 1255, 2411, 2449
HphI GGTGA 4 cut(s) 8, 191, 854, 2242
Hpy166II GTNNAC 4 cut(s) 888, 1683, 1873, 2170
Hpy188I TCNGA 9 cut(s) 660, 910, 946, 1424, 1498, 2010, 2331, 2370, 2403
Hpy8I GTNNAC 4 cut(s) 888, 1683, 1873, 2170
Hpy99I CGWCG 4 cut(s) 227, 260, 278, 299
HpyAV CCTTC 8 cut(s) 102, 541, 1190, 1411, 1691, 1753, 2242, 2341
HpyCH4III ACNGT 8 cut(s) 802, 1608, 1722, 1913, 1952, 2014, 2257, 2382
HpyCH4IV ACGT 2 cut(s) 1563, 2189
HpyF10VI GCNNNNNNNGC 5 cut(s) 57, 165, 334, 437, 1111
HpySE526I ACGT 2 cut(s) 1563, 2189
Hsp92I GRCGYC 2 cut(s) 225, 294
HspAI GCGC 3 cut(s) 575, 1114, 1238
Kpn2I TCCGGA 2 cut(s) 994, 1254
KroI GCCGGC 2 cut(s) 101, 2410
KroNI GCCGGC 2 cut(s) 103, 2412
Kzo9I GATC 7 cut(s) 151, 262, 499, 655, 900, 1588, 2238
LmnI GCTCC 3 cut(s) 35, 1987, 2215
Lsp1109I GCAGC 9 cut(s) 63, 66, 556, 564, 584, 763, 943, 991, 2305
LweI GCATC 7 cut(s) 626, 1051, 1179, 1387, 2013, 2026, 2305
MaeI CTAG 4 cut(s) 1764, 2343, 2459, 2498
MaeII ACGT 2 cut(s) 1563, 2189
MaeIII GTNAC 4 cut(s) 467, 493, 802, 2357
MalI GATC 7 cut(s) 153, 264, 501, 657, 902, 1590, 2240
MboI GATC 7 cut(s) 151, 262, 499, 655, 900, 1588, 2238
MfeI CAATTG 1 cut(s) 2322
MflI RGATCY 2 cut(s) 900, 1588
MhlI GDGCHC 1 cut(s) 2276
MlsI TGGCCA 2 cut(s) 594, 854
MluNI TGGCCA 2 cut(s) 594, 854
MlyI GAGTC 4 cut(s) 853, 1218, 1748, 2365
MmeI TCCRAC 2 cut(s) 1447, 2410
Mox20I TGGCCA 2 cut(s) 594, 854
MroI TCCGGA 2 cut(s) 994, 1254
MroNI GCCGGC 2 cut(s) 101, 2410
MscI TGGCCA 2 cut(s) 594, 854
MslI CAYNNNNRTG 1 cut(s) 2228
Msp20I TGGCCA 2 cut(s) 594, 854
MspA1I CMGCKG 2 cut(s) 57, 1094
MspI CCGG 5 cut(s) 102, 995, 1255, 2411, 2449
MspR9I CCNGG 6 cut(s) 209, 856, 1058, 1760, 1877, 2450
MunI CAATTG 1 cut(s) 2322
Mva1269I GAATGC 2 cut(s) 1521, 2239
MvaI CCWGG 5 cut(s) 209, 856, 1058, 1760, 1877
MwoI GCNNNNNNNGC 5 cut(s) 57, 165, 334, 437, 1111
NaeI GCCGGC 2 cut(s) 103, 2412
NciI CCSGG 1 cut(s) 2450
NcoI CCATGG 1 cut(s) 1839
NdeI CATATG 1 cut(s) 703
NdeII GATC 7 cut(s) 151, 262, 499, 655, 900, 1588, 2238
NgoMIV GCCGGC 2 cut(s) 101, 2410
NlaIV GGNNCC 2 cut(s) 61, 213
NmuCI GTSAC 3 cut(s) 467, 802, 2357
NspI RCATGY 2 cut(s) 1013, 2124
PacI TTAATTAA 1 cut(s) 1416
PagI TCATGA 1 cut(s) 1858
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PctI GAATGC 2 cut(s) 1521, 2239
PdiI GCCGGC 2 cut(s) 103, 2412
PfeI GAWTC 6 cut(s) 461, 670, 1165, 1223, 2069, 2198
PleI GAGTC 4 cut(s) 853, 1217, 1747, 2364
PpsI GAGTC 4 cut(s) 853, 1217, 1747, 2364
PpuMI RGGWCCY 3 cut(s) 211, 646, 1177
PshAI GACNNNNGTC 1 cut(s) 2015
PshBI ATTAAT 1 cut(s) 1416
PsiI TTATAA 1 cut(s) 1581
Psp5II RGGWCCY 3 cut(s) 211, 646, 1177
Psp6I CCWGG 5 cut(s) 207, 854, 1056, 1758, 1875
PspFI CCCAGC 3 cut(s) 155, 324, 1478
PspGI CCWGG 5 cut(s) 207, 854, 1056, 1758, 1875
PspN4I GGNNCC 2 cut(s) 61, 213
PspPI GGNCC 6 cut(s) 104, 211, 270, 322, 646, 1177
PspPPI RGGWCCY 3 cut(s) 211, 646, 1177
PstI CTGCAG 2 cut(s) 574, 2112
PsuI RGATCY 2 cut(s) 900, 1588
RsaI GTAC 6 cut(s) 940, 1069, 1492, 1820, 1910, 2456
RsaNI GTAC 6 cut(s) 939, 1068, 1491, 1819, 1909, 2455
RseI CAYNNNNRTG 1 cut(s) 2228
Sau3AI GATC 7 cut(s) 151, 262, 499, 655, 900, 1588, 2238
Sau96I GGNCC 6 cut(s) 104, 211, 270, 322, 646, 1177
SchI GAGTC 4 cut(s) 853, 1218, 1748, 2365
ScrFI CCNGG 6 cut(s) 209, 856, 1058, 1760, 1877, 2450
SduI GDGCHC 1 cut(s) 2276
SfaNI GCATC 7 cut(s) 626, 1051, 1179, 1387, 2013, 2026, 2305
SfcI CTRYAG 3 cut(s) 570, 960, 2108
SinI GGWCC 3 cut(s) 211, 646, 1177
SmiMI CAYNNNNRTG 1 cut(s) 2228
SspMI CTAG 4 cut(s) 1764, 2343, 2459, 2498
StyD4I CCNGG 6 cut(s) 207, 854, 1056, 1758, 1875, 2448
StyI CCWWGG 1 cut(s) 1839
TaaI ACNGT 8 cut(s) 802, 1608, 1722, 1913, 1952, 2014, 2257, 2382
TaiI ACGT 2 cut(s) 1566, 2192
TaqI TCGA 7 cut(s) 150, 234, 255, 1134, 1404, 1695, 1724
TaqII GACCGA 1 cut(s) 1679
TatI WGTACW 3 cut(s) 938, 1067, 1908
TauI GCSGC 2 cut(s) 60, 302
TfiI GAWTC 6 cut(s) 461, 670, 1165, 1223, 2069, 2198
TscAI CASTG 6 cut(s) 811, 855, 1918, 1955, 2262, 2284
TseFI GTSAC 3 cut(s) 467, 802, 2357
TseI GCWGC 9 cut(s) 51, 54, 569, 572, 577, 751, 956, 979, 2318
Tsp45I GTSAC 3 cut(s) 467, 802, 2357
TspGWI ACGGA 1 cut(s) 553
TspRI CASTG 6 cut(s) 811, 855, 1918, 1955, 2262, 2284
VpaK11BI GGWCC 3 cut(s) 211, 646, 1177
VspI ATTAAT 1 cut(s) 1416
XapI RAATTY 6 cut(s) 394, 947, 1322, 1936, 2396, 2484
XceI RCATGY 2 cut(s) 1013, 2124
XmiI GTMKAC 1 cut(s) 1872
XspI CTAG 4 cut(s) 1764, 2343, 2459, 2498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.