MD10G1263800.v1.1

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016780
Physical Location & Seq
Forward (+)
35774371 .. 35776630
2260 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1263800.v1.1.491

Sequence Viewer

Length: 768 bp
ATGGCGGAGGAGGAGGAGGAGCCGAAGAAGAGAAGGGTGGTGGTGGAATCCCTCGGATGGCTGACGGAGTCCTCAATTATGCCGAAGAAGCACCGTGCCATCGGCGGCGTCGGAGCCTCCTCAATCATGGAGCTCAAGGCCCAACTCTATCAGTCCCAAGAAGAATCCAAGAAGTCCAAAGAACTCGCCGGCTCAGACATCGAGTTCCATCGGGCCAAGAAGAGAATTACCGCACACGACGCCTTCTCCGCCAAGAACTCCGGCGTCGACGCCCGGGCTCACAAGGACAAGCTTGAGCTAAAAGCAGTGAAGGATGGATCGGCGAGCTATGCGGCGTTGGAGAAGAAGGCTGCATTGTACGATAAGCTAGCAAGGGGTGAGCTATCGGATGAAGAAGATAAAGAAAAGTATTGTGTGGATTTTTACGGCAAGAGAGTTGACCATGACAAACCGCAGCAGACACAGCACCATGATTCACCTGCTGTAATATCTCCAGAGAATCAAGATGGAGAGAGCAATGCTTCTACGCTGTTTAGCACGAAACCTTTGGGGCTTGGGCGAGCGGATGCGACAGTGGACAATGATATTCACAAGCGTTTTGTGAGGGAAGTACACGAAGAAGCAAATCAAGCGAGAGAGAAGGCATCTGAGCTCAAACTGCGCAGGGAAGAGCAAGGAGCTTCTCGTCGTGAGAAGTTAAAACAGGCTTATATACGGAAACAGTTAGAGAGACTTAAAGCAGCATCAAGTAATAAGGAACAGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

256

Amino Acids

28.71

Weight (kDa)

8.68

Isoelectric Point (pI)

55.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016780)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05790
malus_domestica MD10G1263800.v1.1
prunus_persica Prupe.4G078700_v2.0.a1
pyrus_communis pycom05g26480 pycom10g22000
rosa_chinensis RchiOBHm_Chr5g0013711
rosa_laevigata RLG00000032042
rosa_multiflora Rmu_sc0002573.1_g000001
rosa_roxburghii Rroxscaffold_1G00062900
rosa_rugosa Rorug05G0012500
rosa_samantha Rh5AG106800 Rh5BG102900 Rh5CG114800 Rh5DG101600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 487
Acc16I TGCGCA 1 cut(s) 662
Acc36I ACCTGC 1 cut(s) 487
AccBSI CCGCTC 1 cut(s) 563
AccI GTMKAC 1 cut(s) 267
AciI CCGC 7 cut(s) 5, 105, 231, 249, 332, 452, 563
AclWI GGATC 1 cut(s) 325
AcyI GRCGYC 4 cut(s) 108, 240, 264, 270
AfaI GTAC 2 cut(s) 359, 612
AfiI CCNNNNNNNGG 1 cut(s) 57
AluBI AGCT 8 cut(s) 133, 292, 298, 327, 367, 382, 652, 680
AluI AGCT 8 cut(s) 133, 292, 298, 327, 367, 382, 652, 680
Alw21I GWGCWC 2 cut(s) 135, 654
Alw26I GTCTC 1 cut(s) 724
AlwI GGATC 1 cut(s) 325
Ama87I CYCGRG 1 cut(s) 273
AoxI GGCC 2 cut(s) 138, 213
ApeKI GCWGC 3 cut(s) 350, 454, 740
AspLEI GCGC 1 cut(s) 663
AspS9I GGNCC 2 cut(s) 139, 213
AsuC2I CCSGG 2 cut(s) 274, 275
AsuHPI GGTGA 2 cut(s) 389, 468
AsuNHI GCTAGC 1 cut(s) 367
AvaI CYCGRG 1 cut(s) 273
BanII GRGCYC 3 cut(s) 135, 280, 654
Bbv12I GWGCWC 2 cut(s) 135, 654
BbvI GCAGC 3 cut(s) 337, 466, 752
BccI CCATC 5 cut(s) 51, 107, 216, 308, 500
BceAI ACGGC 1 cut(s) 442
BcnI CCSGG 2 cut(s) 274, 275
BcoDI GTCTC 1 cut(s) 724
BfaI CTAG 1 cut(s) 368
BfuAI ACCTGC 1 cut(s) 487
BisI GCNGC 5 cut(s) 106, 333, 351, 455, 741
BlsI GCNGC 5 cut(s) 107, 334, 352, 456, 742
Bme1390I CCNGG 2 cut(s) 274, 275
BmeT110I CYCGRG 1 cut(s) 273
BmgT120I GGNCC 2 cut(s) 139, 213
BmiI GGNNCC 2 cut(s) 21, 115
BmrFI CCNGG 2 cut(s) 274, 275
BmsI GCATC 3 cut(s) 556, 653, 752
BmtI GCTAGC 1 cut(s) 371
BpmI CTGGAG 1 cut(s) 477
BpuEI CTTGAG 2 cut(s) 119, 314
BpuMI CCSGG 2 cut(s) 274, 275
BsaHI GRCGYC 4 cut(s) 108, 240, 264, 270
BsaJI CCNNGG 2 cut(s) 52, 273
BsaXI ACNNNNNCTCC 2 cut(s) 230, 260
Bsc4I CCNNNNNNNGG 1 cut(s) 57
Bse118I RCCGGY 1 cut(s) 188
Bse3DI GCAATG 1 cut(s) 523
BseDI CCNNGG 2 cut(s) 52, 273
BseGI GGATG 4 cut(s) 62, 319, 394, 571
BseLI CCNNNNNNNGG 1 cut(s) 57
BseMI GCAATG 1 cut(s) 523
BseMII CTCAG 2 cut(s) 207, 639
BseRI GAGGAG 5 cut(s) 23, 26, 29, 32, 109
BseXI GCAGC 3 cut(s) 337, 466, 752
BshFI GGCC 2 cut(s) 140, 215
BsiHKAI GWGCWC 2 cut(s) 135, 654
BsiHKCI CYCGRG 1 cut(s) 273
BsiSI CCGG 3 cut(s) 189, 261, 274
BslFI GGGAC 1 cut(s) 139
BslI CCNNNNNNNGG 1 cut(s) 57
BsmAI GTCTC 1 cut(s) 724
BsmFI GGGAC 1 cut(s) 139
BsnI GGCC 2 cut(s) 140, 215
BsoBI CYCGRG 1 cut(s) 273
Bsp1286I GDGCHC 3 cut(s) 135, 280, 654
Bsp143I GATC 1 cut(s) 317
BspACI CCGC 7 cut(s) 5, 105, 231, 249, 332, 452, 563
BspANI GGCC 2 cut(s) 140, 215
BspCNI CTCAG 2 cut(s) 206, 640
BspLI GGNNCC 2 cut(s) 21, 115
BspMI ACCTGC 1 cut(s) 487
BspOI GCTAGC 1 cut(s) 371
BspPI GGATC 1 cut(s) 325
BspQI GCTCTTC 1 cut(s) 663
BsrBI CCGCTC 1 cut(s) 563
BsrDI GCAATG 1 cut(s) 523
BsrFI RCCGGY 1 cut(s) 188
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 2 cut(s) 52, 273
BssMI GATC 1 cut(s) 317
BssNI GRCGYC 4 cut(s) 108, 240, 264, 270
Bst4CI ACNGT 3 cut(s) 95, 574, 723
Bst6I CTCTTC 3 cut(s) 23, 215, 663
BstACI GRCGYC 4 cut(s) 108, 240, 264, 270
BstC8I GCNNGC 4 cut(s) 190, 325, 369, 561
BstDEI CTNAG 2 cut(s) 193, 648
BstF5I GGATG 4 cut(s) 62, 319, 394, 571
BstHHI GCGC 1 cut(s) 663
BstKTI GATC 1 cut(s) 320
BstMAI GTCTC 1 cut(s) 724
BstMBI GATC 1 cut(s) 317
BstMWI GCNNNNNNNGC 7 cut(s) 88, 239, 248, 329, 463, 629, 658
BstSCI CCNGG 2 cut(s) 272, 273
BstV1I GCAGC 3 cut(s) 337, 466, 752
BsuRI GGCC 2 cut(s) 140, 215
BtsCI GGATG 4 cut(s) 62, 319, 394, 571
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 2 cut(s) 312, 579
BveI ACCTGC 1 cut(s) 487
Cac8I GCNNGC 4 cut(s) 190, 325, 369, 561
CfoI GCGC 1 cut(s) 663
Cfr10I RCCGGY 1 cut(s) 188
Cfr13I GGNCC 2 cut(s) 139, 213
Cfr9I CCCGGG 1 cut(s) 273
CseI GACGC 4 cut(s) 97, 248, 253, 278
Csp6I GTAC 2 cut(s) 358, 611
CviAII CATG 4 cut(s) 127, 443, 470, 765
CviQI GTAC 2 cut(s) 358, 611
DdeI CTNAG 2 cut(s) 193, 648
DpnI GATC 1 cut(s) 319
DpnII GATC 1 cut(s) 317
Eam1104I CTCTTC 3 cut(s) 23, 215, 663
EarI CTCTTC 3 cut(s) 23, 215, 663
EciI GGCGGA 2 cut(s) 20, 238
Ecl136II GAGCTC 2 cut(s) 133, 652
Eco24I GRGCYC 3 cut(s) 135, 280, 654
Eco53kI GAGCTC 2 cut(s) 133, 652
Eco88I CYCGRG 1 cut(s) 273
EcoICRI GAGCTC 2 cut(s) 133, 652
EcoT38I GRGCYC 3 cut(s) 135, 280, 654
FaeI CATG 4 cut(s) 130, 446, 473, 768
FaiI YATR 8 cut(s) 80, 128, 330, 444, 471, 711, 713, 766
FaqI GGGAC 1 cut(s) 139
FatI CATG 4 cut(s) 126, 442, 469, 764
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 5 cut(s) 106, 333, 351, 455, 741
FokI GGATG 4 cut(s) 69, 326, 401, 578
FriOI GRGCYC 3 cut(s) 135, 280, 654
Fsp4HI GCNGC 5 cut(s) 106, 333, 351, 455, 741
FspBI CTAG 1 cut(s) 368
FspI TGCGCA 1 cut(s) 662
GlaI GCGC 1 cut(s) 662
GluI GCNGC 5 cut(s) 106, 333, 351, 455, 741
GsuI CTGGAG 1 cut(s) 477
HaeIII GGCC 2 cut(s) 140, 215
HapII CCGG 3 cut(s) 189, 261, 274
HgaI GACGC 4 cut(s) 97, 248, 253, 278
HhaI GCGC 1 cut(s) 663
Hin1I GRCGYC 4 cut(s) 108, 240, 264, 270
Hin1II CATG 4 cut(s) 130, 446, 473, 768
Hin6I GCGC 1 cut(s) 661
HinP1I GCGC 1 cut(s) 661
HincII GTYRAC 2 cut(s) 268, 439
HindII GTYRAC 2 cut(s) 268, 439
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 5 cut(s) 47, 68, 164, 473, 499
HpaII CCGG 3 cut(s) 189, 261, 274
HphI GGTGA 2 cut(s) 389, 468
Hpy166II GTNNAC 4 cut(s) 268, 439, 577, 613
Hpy188I TCNGA 5 cut(s) 56, 113, 196, 388, 649
Hpy188III TCNNGA 3 cut(s) 494, 503, 689
Hpy8I GTNNAC 4 cut(s) 268, 439, 577, 613
Hpy99I CGWCG 5 cut(s) 113, 242, 269, 272, 690
HpyAV CCTTC 5 cut(s) 27, 253, 304, 340, 634
HpyCH4III ACNGT 3 cut(s) 95, 574, 723
HpyCH4V TGCA 1 cut(s) 353
HpyF10VI GCNNNNNNNGC 7 cut(s) 88, 239, 248, 329, 463, 629, 658
HpyF3I CTNAG 2 cut(s) 193, 648
Hsp92I GRCGYC 4 cut(s) 108, 240, 264, 270
Hsp92II CATG 4 cut(s) 130, 446, 473, 768
HspAI GCGC 1 cut(s) 661
KroI GCCGGC 1 cut(s) 188
KroNI GCCGGC 1 cut(s) 190
Kzo9I GATC 1 cut(s) 317
LguI GCTCTTC 1 cut(s) 663
LmnI GCTCC 4 cut(s) 19, 113, 130, 677
LpnPI CCDG 7 cut(s) 202, 274, 287, 492, 507, 649, 689
Lsp1109I GCAGC 3 cut(s) 337, 466, 752
LweI GCATC 3 cut(s) 556, 653, 752
MaeI CTAG 1 cut(s) 368
MalI GATC 1 cut(s) 319
MbiI CCGCTC 1 cut(s) 563
MboI GATC 1 cut(s) 317
MhlI GDGCHC 3 cut(s) 135, 280, 654
MluCI AATT 2 cut(s) 75, 225
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 2 cut(s) 91, 318
MnlI CCTC 8 cut(s) 4, 7, 10, 62, 82, 127, 130, 597
MroNI GCCGGC 1 cut(s) 188
MseI TTAA 2 cut(s) 698, 735
MspI CCGG 3 cut(s) 189, 261, 274
MspR9I CCNGG 2 cut(s) 274, 275
MwoI GCNNNNNNNGC 7 cut(s) 88, 239, 248, 329, 463, 629, 658
NaeI GCCGGC 1 cut(s) 190
NciI CCSGG 2 cut(s) 274, 275
NdeII GATC 1 cut(s) 317
NgoMIV GCCGGC 1 cut(s) 188
NheI GCTAGC 1 cut(s) 367
NlaIII CATG 4 cut(s) 130, 446, 473, 768
NlaIV GGNNCC 2 cut(s) 21, 115
NsbI TGCGCA 1 cut(s) 662
PaqCI CACCTGC 1 cut(s) 487
PciSI GCTCTTC 1 cut(s) 663
PdiI GCCGGC 1 cut(s) 190
PfeI GAWTC 4 cut(s) 47, 164, 473, 499
PflFI GACNNNGTC 1 cut(s) 67
PkrI GCNGC 5 cut(s) 107, 334, 352, 456, 742
PleI GAGTC 1 cut(s) 76
PpsI GAGTC 1 cut(s) 76
Psp124BI GAGCTC 2 cut(s) 135, 654
PspN4I GGNNCC 2 cut(s) 21, 115
PspPI GGNCC 2 cut(s) 139, 213
PsyI GACNNNGTC 1 cut(s) 67
RsaI GTAC 2 cut(s) 359, 612
RsaNI GTAC 2 cut(s) 358, 611
SacI GAGCTC 2 cut(s) 135, 654
SalI GTCGAC 1 cut(s) 266
SapI GCTCTTC 1 cut(s) 663
SaqAI TTAA 2 cut(s) 698, 735
SatI GCNGC 5 cut(s) 106, 333, 351, 455, 741
Sau3AI GATC 1 cut(s) 317
Sau96I GGNCC 2 cut(s) 139, 213
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 2 cut(s) 274, 275
SduI GDGCHC 3 cut(s) 135, 280, 654
SfaNI GCATC 3 cut(s) 556, 653, 752
SgrDI CGTCGACG 1 cut(s) 266
SmaI CCCGGG 1 cut(s) 275
SmlI CTYRAG 2 cut(s) 134, 293
SmoI CTYRAG 2 cut(s) 134, 293
SrfI GCCCGGGC 1 cut(s) 275
Sse9I AATT 2 cut(s) 75, 225
SsiI CCGC 7 cut(s) 5, 105, 231, 249, 332, 452, 563
SspMI CTAG 1 cut(s) 368
SstI GAGCTC 2 cut(s) 135, 654
StyD4I CCNGG 2 cut(s) 272, 273
TaaI ACNGT 3 cut(s) 95, 574, 723
TaqI TCGA 2 cut(s) 201, 267
TasI AATT 2 cut(s) 75, 225
TatI WGTACW 1 cut(s) 610
TauI GCSGC 2 cut(s) 108, 335
TfiI GAWTC 4 cut(s) 47, 164, 473, 499
Tru1I TTAA 2 cut(s) 698, 735
Tru9I TTAA 2 cut(s) 698, 735
TscAI CASTG 2 cut(s) 312, 579
TseI GCWGC 3 cut(s) 350, 454, 740
TspDTI ATGAA 1 cut(s) 405
TspGWI ACGGA 2 cut(s) 80, 730
TspMI CCCGGG 1 cut(s) 273
TspRI CASTG 2 cut(s) 312, 579
Tth111I GACNNNGTC 1 cut(s) 67
XmaI CCCGGG 1 cut(s) 273
XmiI GTMKAC 1 cut(s) 267
XspI CTAG 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.