RLG00000032042

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016780
Physical Location & Seq
Reverse (-)
10390314 .. 10391839
1526 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032042

Sequence Viewer

Length: 771 bp
ATGGGAGAAGAAGAAGAAGAGCCCAAGAAGAAGAGGGTGGTGGTGGAATCCCTGGGATGGCTTACGGAGTCGACCATAATGCCCAAGAAGCACCGCGCCATCGCCGGCGTCGGAGCCTCTTCTATCATGGAGCTCAAGGCCCAGCTCTACCAGTCTCAAGAAGACTCCAAGAAGTCCAAGGACCTCGCCGGCCCAGACGCCGAGTTCCACCGCGCCAAGAAGAAGATCGCTGCACACGATGCCTTCTCCGTCAAGAACTCCGGCGTCGACTCCCGGGCCCACAAGGACAAGCTTGAGCTGAAGGCAGTTAAGGATGGATCAGCGAGCTATGCAGCGTTGGAGAAAAAGGCTGCATTGTATGATAAGCTAGCGAGGGGAGAGCTATCCGATGAGGAAGACAAGGAAAAGTATTGCGTGGATTTCTTTGTCAAGAGACATGAGCAGGACGAGTCAGAGGAAACTCAGCATCACGATTCGTCTGTTGTTGCACCACCGGAGAATCAAGATGGAGGGACTGATCTTTCTATGTTGTTTAGCTCAAAACCTGTTGGGCTTGGGCAGGCAGGTGCAATATGGGACAATGATGAACACAAACGATTTGTGAGGGAAGTTCATCAAGAAGCGAATCAAGCAAGAGAGAGAGCCTCAGAGCTCAAAGTACGCAGGCAAGAGCAAGCAACTGCTCGTCGTGAGAAGTTAAAACAAGCATACATACGGAAACAGCTAGAGAAACTAAAAGCAGCAGCACCTAATAAGGAACAGATAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

257

Amino Acids

28.84

Weight (kDa)

7.79

Isoelectric Point (pI)

49.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016780)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05790
malus_domestica MD10G1263800.v1.1
prunus_persica Prupe.4G078700_v2.0.a1
pyrus_communis pycom05g26480 pycom10g22000
rosa_chinensis RchiOBHm_Chr5g0013711
rosa_laevigata RLG00000032042
rosa_multiflora Rmu_sc0002573.1_g000001
rosa_roxburghii Rroxscaffold_1G00062900
rosa_rugosa Rorug05G0012500
rosa_samantha Rh5AG106800 Rh5BG102900 Rh5CG114800 Rh5DG101600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 554
Acc36I ACCTGC 1 cut(s) 554
AccI GTMKAC 2 cut(s) 71, 267
AccII CGCG 2 cut(s) 96, 213
AciI CCGC 2 cut(s) 94, 211
AclWI GGATC 1 cut(s) 325
AcuI CTGAAG 1 cut(s) 320
AcyI GRCGYC 3 cut(s) 108, 198, 264
AfaI GTAC 1 cut(s) 660
AfiI CCNNNNNNNGG 1 cut(s) 57
AjnI CCWGG 1 cut(s) 51
Alw21I GWGCWC 2 cut(s) 135, 654
Alw26I GTCTC 2 cut(s) 159, 427
AlwI GGATC 1 cut(s) 325
Ama87I CYCGRG 1 cut(s) 273
AoxI GGCC 3 cut(s) 138, 190, 276
ApaI GGGCCC 1 cut(s) 280
ApeKI GCWGC 5 cut(s) 230, 332, 350, 740, 743
AspLEI GCGC 2 cut(s) 98, 215
AspS9I GGNCC 5 cut(s) 139, 181, 191, 276, 277
AsuC2I CCSGG 2 cut(s) 274, 275
AsuNHI GCTAGC 1 cut(s) 367
AvaI CYCGRG 1 cut(s) 273
AvaII GGWCC 1 cut(s) 181
BaeGI GKGCMC 1 cut(s) 280
BanII GRGCYC 4 cut(s) 24, 135, 280, 654
BbsI GAAGAC 2 cut(s) 168, 402
Bbv12I GWGCWC 2 cut(s) 135, 654
BbvI GCAGC 5 cut(s) 217, 337, 344, 752, 755
BccI CCATC 4 cut(s) 51, 107, 308, 500
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BciT130I CCWGG 1 cut(s) 53
BcnI CCSGG 2 cut(s) 274, 275
BcoDI GTCTC 2 cut(s) 159, 427
BfaI CTAG 2 cut(s) 368, 725
BfuAI ACCTGC 1 cut(s) 554
BisI GCNGC 5 cut(s) 231, 333, 351, 741, 744
BlsI GCNGC 5 cut(s) 232, 334, 352, 742, 745
Bme1390I CCNGG 3 cut(s) 53, 274, 275
Bme18I GGWCC 1 cut(s) 181
BmeT110I CYCGRG 1 cut(s) 273
BmgT120I GGNCC 5 cut(s) 139, 181, 191, 276, 277
BmiI GGNNCC 2 cut(s) 115, 278
BmrFI CCNGG 3 cut(s) 53, 274, 275
BmsI GCATC 2 cut(s) 229, 475
BmtI GCTAGC 1 cut(s) 371
BpiI GAAGAC 2 cut(s) 168, 402
BplI GAGNNNNNCTC 2 cut(s) 629, 661
BpuEI CTTGAG 3 cut(s) 119, 141, 314
BpuMI CCSGG 2 cut(s) 274, 275
BsaHI GRCGYC 3 cut(s) 108, 198, 264
BsaJI CCNNGG 4 cut(s) 51, 52, 177, 273
BsaWI WCCGGW 1 cut(s) 493
Bsc4I CCNNNNNNNGG 1 cut(s) 57
Bse118I RCCGGY 2 cut(s) 104, 188
Bse1I ACTGG 1 cut(s) 151
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 4 cut(s) 51, 52, 177, 273
BseGI GGATG 2 cut(s) 62, 319
BseLI CCNNNNNNNGG 1 cut(s) 57
BseMII CTCAG 2 cut(s) 476, 660
BseNI ACTGG 1 cut(s) 151
BseSI GKGCMC 1 cut(s) 280
BseXI GCAGC 5 cut(s) 217, 337, 344, 752, 755
BseYI CCCAGC 1 cut(s) 141
BsgI GTGCAG 1 cut(s) 216
Bsh1236I CGCG 2 cut(s) 96, 213
BshFI GGCC 3 cut(s) 140, 192, 278
BsiHKAI GWGCWC 2 cut(s) 135, 654
BsiHKCI CYCGRG 1 cut(s) 273
BsiSI CCGG 5 cut(s) 105, 189, 261, 274, 494
BslFI GGGAC 2 cut(s) 526, 590
BslI CCNNNNNNNGG 1 cut(s) 57
BsmAI GTCTC 2 cut(s) 159, 427
BsmFI GGGAC 2 cut(s) 526, 590
BsnI GGCC 3 cut(s) 140, 192, 278
BsoBI CYCGRG 1 cut(s) 273
Bsp120I GGGCCC 1 cut(s) 276
Bsp1286I GDGCHC 4 cut(s) 24, 135, 280, 654
Bsp143I GATC 3 cut(s) 225, 317, 517
BspACI CCGC 2 cut(s) 94, 211
BspANI GGCC 3 cut(s) 140, 192, 278
BspCNI CTCAG 2 cut(s) 475, 659
BspFNI CGCG 2 cut(s) 96, 213
BspLI GGNNCC 2 cut(s) 115, 278
BspMI ACCTGC 1 cut(s) 554
BspOI GCTAGC 1 cut(s) 371
BspPI GGATC 1 cut(s) 325
BspQI GCTCTTC 1 cut(s) 12
BsrFI RCCGGY 2 cut(s) 104, 188
BsrI ACTGG 1 cut(s) 151
BssAI RCCGGY 2 cut(s) 104, 188
BssECI CCNNGG 4 cut(s) 51, 52, 177, 273
BssMI GATC 3 cut(s) 225, 317, 517
BssNI GRCGYC 3 cut(s) 108, 198, 264
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 1 cut(s) 53
Bst6I CTCTTC 3 cut(s) 12, 26, 124
BstACI GRCGYC 3 cut(s) 108, 198, 264
BstAPI GCANNNNNTGC 1 cut(s) 239
BstC8I GCNNGC 7 cut(s) 106, 190, 325, 369, 561, 665, 675
BstDEI CTNAG 2 cut(s) 462, 646
BstF5I GGATG 2 cut(s) 62, 319
BstFNI CGCG 2 cut(s) 96, 213
BstHHI GCGC 2 cut(s) 98, 215
BstKTI GATC 3 cut(s) 228, 320, 520
BstMAI GTCTC 2 cut(s) 159, 427
BstMBI GATC 3 cut(s) 225, 317, 517
BstMWI GCNNNNNNNGC 4 cut(s) 88, 239, 329, 629
BstNI CCWGG 1 cut(s) 53
BstSCI CCNGG 3 cut(s) 51, 272, 273
BstSLI GKGCMC 1 cut(s) 280
BstUI CGCG 2 cut(s) 96, 213
BstV1I GCAGC 5 cut(s) 217, 337, 344, 752, 755
BstV2I GAAGAC 2 cut(s) 168, 402
BsuRI GGCC 3 cut(s) 140, 192, 278
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 2 cut(s) 62, 319
BveI ACCTGC 1 cut(s) 554
Cac8I GCNNGC 7 cut(s) 106, 190, 325, 369, 561, 665, 675
CfoI GCGC 2 cut(s) 98, 215
Cfr10I RCCGGY 2 cut(s) 104, 188
Cfr13I GGNCC 5 cut(s) 139, 181, 191, 276, 277
Cfr9I CCCGGG 1 cut(s) 273
CseI GACGC 3 cut(s) 97, 206, 253
Csp6I GTAC 1 cut(s) 659
CviAII CATG 2 cut(s) 127, 437
CviQI GTAC 1 cut(s) 659
DdeI CTNAG 2 cut(s) 462, 646
DpnI GATC 3 cut(s) 227, 319, 519
DpnII GATC 3 cut(s) 225, 317, 517
Eam1104I CTCTTC 3 cut(s) 12, 26, 124
EarI CTCTTC 3 cut(s) 12, 26, 124
Ecl136II GAGCTC 2 cut(s) 133, 652
Eco130I CCWWGG 1 cut(s) 177
Eco24I GRGCYC 4 cut(s) 24, 135, 280, 654
Eco47I GGWCC 1 cut(s) 181
Eco53kI GAGCTC 2 cut(s) 133, 652
Eco57I CTGAAG 1 cut(s) 320
Eco88I CYCGRG 1 cut(s) 273
EcoICRI GAGCTC 2 cut(s) 133, 652
EcoO109I RGGNCCY 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 177
EcoT38I GRGCYC 4 cut(s) 24, 135, 280, 654
ErhI CCWWGG 1 cut(s) 177
FaeI CATG 2 cut(s) 130, 440
FaiI YATR 9 cut(s) 77, 128, 330, 360, 438, 527, 574, 709, 713
FaqI GGGAC 2 cut(s) 526, 590
FatI CATG 2 cut(s) 126, 436
FblI GTMKAC 2 cut(s) 71, 267
Fnu4HI GCNGC 5 cut(s) 231, 333, 351, 741, 744
FokI GGATG 2 cut(s) 69, 326
FriOI GRGCYC 4 cut(s) 24, 135, 280, 654
Fsp4HI GCNGC 5 cut(s) 231, 333, 351, 741, 744
FspBI CTAG 2 cut(s) 368, 725
GlaI GCGC 2 cut(s) 97, 214
GluI GCNGC 5 cut(s) 231, 333, 351, 741, 744
GsaI CCCAGC 1 cut(s) 145
HaeIII GGCC 3 cut(s) 140, 192, 278
HapII CCGG 5 cut(s) 105, 189, 261, 274, 494
HgaI GACGC 3 cut(s) 97, 206, 253
HhaI GCGC 2 cut(s) 98, 215
Hin1I GRCGYC 3 cut(s) 108, 198, 264
Hin1II CATG 2 cut(s) 130, 440
Hin6I GCGC 2 cut(s) 96, 213
HinP1I GCGC 2 cut(s) 96, 213
HincII GTYRAC 2 cut(s) 72, 268
HindII GTYRAC 2 cut(s) 72, 268
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 8 cut(s) 47, 68, 164, 269, 449, 473, 499, 625
HpaII CCGG 5 cut(s) 105, 189, 261, 274, 494
Hpy166II GTNNAC 2 cut(s) 72, 268
Hpy188I TCNGA 4 cut(s) 113, 388, 454, 649
Hpy188III TCNNGA 7 cut(s) 158, 253, 430, 470, 503, 617, 689
Hpy8I GTNNAC 2 cut(s) 72, 268
Hpy99I CGWCG 3 cut(s) 113, 269, 690
HpyAV CCTTC 2 cut(s) 253, 295
HpyCH4V TGCA 5 cut(s) 233, 332, 353, 488, 569
HpyF10VI GCNNNNNNNGC 4 cut(s) 88, 239, 329, 629
HpyF3I CTNAG 2 cut(s) 462, 646
Hsp92I GRCGYC 3 cut(s) 108, 198, 264
Hsp92II CATG 2 cut(s) 130, 440
HspAI GCGC 2 cut(s) 96, 213
KroI GCCGGC 2 cut(s) 104, 188
KroNI GCCGGC 2 cut(s) 106, 190
Kzo9I GATC 3 cut(s) 225, 317, 517
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 2 cut(s) 113, 130
Lsp1109I GCAGC 5 cut(s) 217, 337, 344, 752, 755
LweI GCATC 2 cut(s) 229, 475
MaeI CTAG 2 cut(s) 368, 725
MalI GATC 3 cut(s) 227, 319, 519
MboI GATC 3 cut(s) 225, 317, 517
MhlI GDGCHC 4 cut(s) 24, 135, 280, 654
MlyI GAGTC 4 cut(s) 77, 158, 263, 458
MmeI TCCRAC 2 cut(s) 91, 318
MnlI CCTC 9 cut(s) 27, 127, 194, 366, 385, 448, 503, 597, 655
MreI CGCCGGCG 1 cut(s) 104
MroNI GCCGGC 2 cut(s) 104, 188
MseI TTAA 2 cut(s) 309, 698
MspI CCGG 5 cut(s) 105, 189, 261, 274, 494
MspR9I CCNGG 3 cut(s) 53, 274, 275
MvaI CCWGG 1 cut(s) 53
MvnI CGCG 2 cut(s) 96, 213
MwoI GCNNNNNNNGC 4 cut(s) 88, 239, 329, 629
NaeI GCCGGC 2 cut(s) 106, 190
NciI CCSGG 2 cut(s) 274, 275
NdeII GATC 3 cut(s) 225, 317, 517
NgoMIV GCCGGC 2 cut(s) 104, 188
NheI GCTAGC 1 cut(s) 367
NlaIII CATG 2 cut(s) 130, 440
NlaIV GGNNCC 2 cut(s) 115, 278
NmeAIII GCCGAG 1 cut(s) 226
PaqCI CACCTGC 1 cut(s) 554
PasI CCCWGGG 1 cut(s) 52
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PdiI GCCGGC 2 cut(s) 106, 190
PfeI GAWTC 4 cut(s) 47, 473, 499, 625
PkrI GCNGC 5 cut(s) 232, 334, 352, 742, 745
PleI GAGTC 4 cut(s) 76, 158, 263, 457
PpsI GAGTC 4 cut(s) 76, 158, 263, 457
PpuMI RGGWCCY 1 cut(s) 181
Psp124BI GAGCTC 2 cut(s) 135, 654
Psp5II RGGWCCY 1 cut(s) 181
Psp6I CCWGG 1 cut(s) 51
PspFI CCCAGC 1 cut(s) 141
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 2 cut(s) 115, 278
PspOMI GGGCCC 1 cut(s) 276
PspPI GGNCC 5 cut(s) 139, 181, 191, 276, 277
PspPPI RGGWCCY 1 cut(s) 181
RsaI GTAC 1 cut(s) 660
RsaNI GTAC 1 cut(s) 659
SacI GAGCTC 2 cut(s) 135, 654
SalI GTCGAC 2 cut(s) 70, 266
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 309, 698
SatI GCNGC 5 cut(s) 231, 333, 351, 741, 744
Sau3AI GATC 3 cut(s) 225, 317, 517
Sau96I GGNCC 5 cut(s) 139, 181, 191, 276, 277
SchI GAGTC 4 cut(s) 77, 158, 263, 458
ScrFI CCNGG 3 cut(s) 53, 274, 275
SduI GDGCHC 4 cut(s) 24, 135, 280, 654
SfaNI GCATC 2 cut(s) 229, 475
SgrAI CRCCGGYG 1 cut(s) 104
SinI GGWCC 1 cut(s) 181
SmaI CCCGGG 1 cut(s) 275
SmlI CTYRAG 3 cut(s) 134, 156, 293
SmoI CTYRAG 3 cut(s) 134, 156, 293
SsiI CCGC 2 cut(s) 94, 211
SspMI CTAG 2 cut(s) 368, 725
SstI GAGCTC 2 cut(s) 135, 654
StyD4I CCNGG 3 cut(s) 51, 272, 273
StyI CCWWGG 1 cut(s) 177
TaqI TCGA 2 cut(s) 71, 267
TfiI GAWTC 4 cut(s) 47, 473, 499, 625
Tru1I TTAA 2 cut(s) 309, 698
Tru9I TTAA 2 cut(s) 309, 698
TseI GCWGC 5 cut(s) 230, 332, 350, 740, 743
TspDTI ATGAA 2 cut(s) 600, 602
TspGWI ACGGA 3 cut(s) 80, 238, 730
TspMI CCCGGG 1 cut(s) 273
VpaK11BI GGWCC 1 cut(s) 181
XmaI CCCGGG 1 cut(s) 273
XmiI GTMKAC 2 cut(s) 71, 267
XspI CTAG 2 cut(s) 368, 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.