MD10G1271700.v1.1

Protein of unknown function (DUF1264)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016212
Physical Location & Seq
Reverse (-)
36354139 .. 36355193
1055 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1271700.v1.1.491

Sequence Viewer

Length: 747 bp
ATGGCTTCAAGTGACAAGACACCGACTCCTAACCCCGCCTCCAGGCTGGGAGATCAGCCAACGCCTCCGGGGAAGCCAATGACGATGGGACAGCACGTCCTGGACAAGGGCGCCGAGATGATGCAGTCCCTCAAGCCGGTGAACCAGATGAGCCAGCACGTGTGCACTTTTGCGCTCTACAGCCACGACATGACTCGTCAGATCGAGACCCACCACTACGTCACTCGCCTCAACCAGGACTTCCTCCAGTGCGCCGTCTACGACTCCGACGACTCCCACGGCCGCCTAATCGGTGTAGAATATATTGTGTCTGATAAGATATTTGAGTCGCTGCCTCCGGATGAGCAAAAGCTTTGGCATTCGCATGCTTACGAGATCAAAGCCGGCCTCTGGGTCAATCCTCGGATCCCCGAGATGATTGGCCAACCTGAGCTCCAGAATTTGGCCAAAACTTACGGAAAATTCTGGTGTACATGGCAAGTTGACCGAGGTGACCGACTTCCACTGGGAGCACCGGCCCTTATGATGTCACCTCAGCCGGTGAATTTGGGTATGGTGAAGCCGGAGCTGTTGGAAACGAGGGATGCCAAATACAACATATCAACGGATGCCTTGAAGACATCGAGGGTGGAGATTGCGGAGCCAGAGTGGATCAATCCGCAGGCGGACTACTGGAAGCTGCATGGCAAGGGTTTTGCGGTGGACATTAAGCAAACTGAAATGAAGAAAAGCACACCCTTTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1264

Protein Analysis

249

Amino Acids

28.13

Weight (kDa)

6.13

Isoelectric Point (pI)

42.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1264 PF06884 45 - 212 1.7e-69 Protein of unknown function (DUF1264)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45690
fragaria_vesca FvH4_3g06760
malus_domestica MD10G1271700.v1.1 MD17G1093800.v1.1
prunus_persica Prupe.4G070200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0012391
rosa_laevigata RLG00000031910
rosa_multiflora Rmu_sc0003469.1_g000010
rosa_roxburghii Rroxscaffold_1G00064270
rosa_rugosa Rorug05G0000800
rosa_samantha Rh5AG095200 Rh5BG091500 Rh5CG104400 Rh5DG090900
rosa_wichuraiana Rw5G008330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 110
AccB7I CCANNNNNTGG 1 cut(s) 442
AccI GTMKAC 1 cut(s) 258
AccIII TCCGGA 1 cut(s) 337
AciI CCGC 6 cut(s) 36, 283, 638, 659, 665, 698
AclWI GGATC 3 cut(s) 400, 413, 659
AcoI YGGCCR 3 cut(s) 280, 421, 444
AcsI RAATTY 3 cut(s) 439, 461, 544
AcvI CACGTG 1 cut(s) 160
AcyI GRCGYC 1 cut(s) 111
AfaI GTAC 1 cut(s) 472
AfiI CCNNNNNNNGG 6 cut(s) 42, 106, 136, 235, 390, 442
AflIII ACRYGT 1 cut(s) 159
AgsI TTSAA 2 cut(s) 9, 616
AhdI GACNNNNNGTC 1 cut(s) 95
AjiI CACGTC 1 cut(s) 97
AjnI CCWGG 3 cut(s) 41, 99, 234
AluBI AGCT 4 cut(s) 352, 433, 568, 679
AluI AGCT 4 cut(s) 352, 433, 568, 679
Alw21I GWGCWC 3 cut(s) 167, 435, 514
Alw26I GTCTC 1 cut(s) 200
Alw44I GTGCAC 1 cut(s) 163
AlwI GGATC 3 cut(s) 400, 413, 659
Ama87I CYCGRG 1 cut(s) 410
Aor13HI TCCGGA 1 cut(s) 337
AoxI GGCC 5 cut(s) 280, 385, 421, 444, 516
ApaLI GTGCAC 1 cut(s) 163
ApeKI GCWGC 2 cut(s) 331, 679
ApoI RAATTY 3 cut(s) 439, 461, 544
AspLEI GCGC 3 cut(s) 113, 175, 254
AspS9I GGNCC 1 cut(s) 517
AsuC2I CCSGG 1 cut(s) 69
AsuHPI GGTGA 5 cut(s) 151, 503, 522, 553, 568
AvaI CYCGRG 1 cut(s) 410
BaeGI GKGCMC 1 cut(s) 167
BalI TGGCCA 2 cut(s) 423, 446
BamHI GGATCC 1 cut(s) 405
BanI GGYRCC 1 cut(s) 110
BanII GRGCYC 1 cut(s) 435
BbrPI CACGTG 1 cut(s) 160
BbsI GAAGAC 1 cut(s) 623
Bbv12I GWGCWC 3 cut(s) 167, 435, 514
BbvCI CCTCAGC 1 cut(s) 534
BbvI GCAGC 2 cut(s) 318, 666
BccI CCATC 1 cut(s) 79
BceAI ACGGC 2 cut(s) 239, 295
BciT130I CCWGG 3 cut(s) 43, 101, 236
BcnI CCSGG 1 cut(s) 69
BcoDI GTCTC 1 cut(s) 200
BfmI CTRYAG 1 cut(s) 178
BfoI RGCGCY 1 cut(s) 114
BisI GCNGC 3 cut(s) 283, 332, 680
BlsI GCNGC 3 cut(s) 284, 333, 681
Bme1390I CCNGG 4 cut(s) 43, 69, 101, 236
BmeRI GACNNNNNGTC 1 cut(s) 95
BmeT110I CYCGRG 1 cut(s) 410
BmgBI CACGTC 1 cut(s) 97
BmgT120I GGNCC 1 cut(s) 517
BmiI GGNNCC 3 cut(s) 112, 407, 642
BmrFI CCNGG 4 cut(s) 43, 69, 101, 236
BmrI ACTGGG 1 cut(s) 515
BmsI GCATC 3 cut(s) 111, 574, 598
BmuI ACTGGG 1 cut(s) 515
BpiI GAAGAC 1 cut(s) 623
BpmI CTGGAG 3 cut(s) 25, 230, 419
Bpu10I CCTNAGC 2 cut(s) 429, 534
BpuEI CTTGAG 1 cut(s) 116
BpuMI CCSGG 1 cut(s) 69
BsaAI YACGTR 1 cut(s) 160
BsaHI GRCGYC 1 cut(s) 111
BsaI GGTCTC 1 cut(s) 200
BsaJI CCNNGG 4 cut(s) 68, 277, 401, 487
BsaWI WCCGGW 1 cut(s) 337
BsaXI ACNNNNNCTCC 8 cut(s) 10, 23, 40, 53, 417, 447, 632, 662
Bsc4I CCNNNNNNNGG 6 cut(s) 42, 106, 136, 235, 390, 442
Bse118I RCCGGY 4 cut(s) 136, 383, 514, 538
Bse1I ACTGG 3 cut(s) 247, 510, 677
BseAI TCCGGA 1 cut(s) 337
BseBI CCWGG 3 cut(s) 43, 101, 236
BseDI CCNNGG 4 cut(s) 68, 277, 401, 487
BseGI GGATG 3 cut(s) 346, 589, 613
BseLI CCNNNNNNNGG 6 cut(s) 42, 106, 136, 235, 390, 442
BseMII CTCAG 2 cut(s) 420, 548
BseNI ACTGG 3 cut(s) 247, 510, 677
BseSI GKGCMC 1 cut(s) 167
BseX3I CGGCCG 1 cut(s) 280
BseXI GCAGC 2 cut(s) 318, 666
BseYI CCCAGC 1 cut(s) 46
Bsh1285I CGRYCG 1 cut(s) 283
BshFI GGCC 5 cut(s) 282, 387, 423, 446, 518
BshNI GGYRCC 1 cut(s) 110
BsiEI CGRYCG 1 cut(s) 283
BsiHKAI GWGCWC 3 cut(s) 167, 435, 514
BsiHKCI CYCGRG 1 cut(s) 410
BsiSI CCGG 7 cut(s) 68, 137, 338, 384, 515, 539, 563
BslFI GGGAC 2 cut(s) 102, 112
BslI CCNNNNNNNGG 6 cut(s) 42, 106, 136, 235, 390, 442
BsmAI GTCTC 1 cut(s) 200
BsmFI GGGAC 2 cut(s) 102, 112
BsmI GAATGC 1 cut(s) 358
BsnI GGCC 5 cut(s) 282, 387, 423, 446, 518
Bso31I GGTCTC 1 cut(s) 200
BsoBI CYCGRG 1 cut(s) 410
Bsp1286I GDGCHC 3 cut(s) 167, 435, 514
Bsp13I TCCGGA 1 cut(s) 337
Bsp1407I TGTACA 1 cut(s) 470
Bsp143I GATC 5 cut(s) 52, 201, 375, 405, 651
BspACI CCGC 6 cut(s) 36, 283, 638, 659, 665, 698
BspANI GGCC 5 cut(s) 282, 387, 423, 446, 518
BspCNI CTCAG 2 cut(s) 421, 547
BspEI TCCGGA 1 cut(s) 337
BspLI GGNNCC 3 cut(s) 112, 407, 642
BspPI GGATC 3 cut(s) 400, 413, 659
BspT107I GGYRCC 1 cut(s) 110
BspTNI GGTCTC 1 cut(s) 200
BsrFI RCCGGY 4 cut(s) 136, 383, 514, 538
BsrGI TGTACA 1 cut(s) 470
BsrI ACTGG 3 cut(s) 247, 510, 677
BssAI RCCGGY 4 cut(s) 136, 383, 514, 538
BssECI CCNNGG 4 cut(s) 68, 277, 401, 487
BssMI GATC 5 cut(s) 52, 201, 375, 405, 651
BssNI GRCGYC 1 cut(s) 111
Bst2UI CCWGG 3 cut(s) 43, 101, 236
BstACI GRCGYC 1 cut(s) 111
BstAUI TGTACA 1 cut(s) 470
BstBAI YACGTR 1 cut(s) 160
BstC8I GCNNGC 4 cut(s) 155, 366, 385, 663
BstDEI CTNAG 3 cut(s) 429, 534, 744
BstDSI CCRYGG 1 cut(s) 277
BstEII GGTNACC 1 cut(s) 491
BstENI CCTNNNNNAGG 2 cut(s) 104, 233
BstF5I GGATG 3 cut(s) 346, 589, 613
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 3 cut(s) 113, 175, 254
BstKTI GATC 5 cut(s) 55, 204, 378, 408, 654
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 5 cut(s) 52, 201, 375, 405, 651
BstMCI CGRYCG 1 cut(s) 283
BstNI CCWGG 3 cut(s) 43, 101, 236
BstNSI RCATGY 1 cut(s) 368
BstPI GGTNACC 1 cut(s) 491
BstSCI CCNGG 4 cut(s) 41, 67, 99, 234
BstSFI CTRYAG 1 cut(s) 178
BstSLI GKGCMC 1 cut(s) 167
BstV1I GCAGC 2 cut(s) 318, 666
BstV2I GAAGAC 1 cut(s) 623
BstX2I RGATCY 1 cut(s) 405
BstYI RGATCY 1 cut(s) 405
BstZI CGGCCG 1 cut(s) 280
BsuRI GGCC 5 cut(s) 282, 387, 423, 446, 518
BtgI CCRYGG 1 cut(s) 277
BtrI CACGTC 1 cut(s) 97
BtsCI GGATG 3 cut(s) 346, 589, 613
BtsIMutI CAGTG 2 cut(s) 254, 503
Cac8I GCNNGC 4 cut(s) 155, 366, 385, 663
CfoI GCGC 3 cut(s) 113, 175, 254
Cfr10I RCCGGY 4 cut(s) 136, 383, 514, 538
Cfr13I GGNCC 1 cut(s) 517
Csp6I GTAC 1 cut(s) 471
CviAII CATG 4 cut(s) 190, 365, 474, 683
CviQI GTAC 1 cut(s) 471
DdeI CTNAG 3 cut(s) 429, 534, 744
DinI GGCGCC 1 cut(s) 112
DpnI GATC 5 cut(s) 54, 203, 377, 407, 653
DpnII GATC 5 cut(s) 52, 201, 375, 405, 651
DriI GACNNNNNGTC 1 cut(s) 95
EaeI YGGCCR 3 cut(s) 280, 421, 444
EagI CGGCCG 1 cut(s) 280
Eam1105I GACNNNNNGTC 1 cut(s) 95
EciI GGCGGA 1 cut(s) 680
Ecl136II GAGCTC 1 cut(s) 433
EclXI CGGCCG 1 cut(s) 280
Eco24I GRGCYC 1 cut(s) 435
Eco31I GGTCTC 1 cut(s) 200
Eco52I CGGCCG 1 cut(s) 280
Eco53kI GAGCTC 1 cut(s) 433
Eco72I CACGTG 1 cut(s) 160
Eco88I CYCGRG 1 cut(s) 410
Eco91I GGTNACC 1 cut(s) 491
EcoICRI GAGCTC 1 cut(s) 433
EcoNI CCTNNNNNAGG 2 cut(s) 104, 233
EcoO65I GGTNACC 1 cut(s) 491
EcoRII CCWGG 3 cut(s) 41, 99, 234
EcoT38I GRGCYC 1 cut(s) 435
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
FaeI CATG 4 cut(s) 193, 368, 477, 686
FaiI YATR 8 cut(s) 191, 303, 366, 475, 524, 554, 599, 684
FaqI GGGAC 2 cut(s) 102, 112
FatI CATG 4 cut(s) 189, 364, 473, 682
FauI CCCGC 1 cut(s) 43
FblI GTMKAC 1 cut(s) 258
Fnu4HI GCNGC 3 cut(s) 283, 332, 680
FokI GGATG 3 cut(s) 353, 596, 620
FriOI GRGCYC 1 cut(s) 435
Fsp4HI GCNGC 3 cut(s) 283, 332, 680
GlaI GCGC 3 cut(s) 112, 174, 253
GluI GCNGC 3 cut(s) 283, 332, 680
GsaI CCCAGC 1 cut(s) 50
GsuI CTGGAG 3 cut(s) 25, 230, 419
HaeII RGCGCY 1 cut(s) 114
HaeIII GGCC 5 cut(s) 282, 387, 423, 446, 518
HapII CCGG 7 cut(s) 68, 137, 338, 384, 515, 539, 563
HhaI GCGC 3 cut(s) 113, 175, 254
Hin1I GRCGYC 1 cut(s) 111
Hin1II CATG 4 cut(s) 193, 368, 477, 686
Hin6I GCGC 3 cut(s) 111, 173, 252
HinP1I GCGC 3 cut(s) 111, 173, 252
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
HindIII AAGCTT 1 cut(s) 350
HinfI GANTC 5 cut(s) 25, 193, 263, 272, 326
HpaII CCGG 7 cut(s) 68, 137, 338, 384, 515, 539, 563
HphI GGTGA 5 cut(s) 151, 503, 522, 553, 568
Hpy166II GTNNAC 6 cut(s) 142, 165, 259, 471, 484, 703
Hpy188I TCNGA 4 cut(s) 201, 268, 313, 405
Hpy188III TCNNGA 3 cut(s) 205, 338, 436
Hpy8I GTNNAC 6 cut(s) 142, 165, 259, 471, 484, 703
Hpy99I CGWCG 1 cut(s) 272
HpyCH4IV ACGT 3 cut(s) 96, 159, 219
HpyCH4V TGCA 3 cut(s) 124, 165, 682
HpyF3I CTNAG 3 cut(s) 429, 534, 744
HpySE526I ACGT 3 cut(s) 96, 159, 219
Hsp92I GRCGYC 1 cut(s) 111
Hsp92II CATG 4 cut(s) 193, 368, 477, 686
HspAI GCGC 3 cut(s) 111, 173, 252
KasI GGCGCC 1 cut(s) 110
Kpn2I TCCGGA 1 cut(s) 337
KroI GCCGGC 1 cut(s) 383
KroNI GCCGGC 1 cut(s) 385
Kzo9I GATC 5 cut(s) 52, 201, 375, 405, 651
LmnI GCTCC 4 cut(s) 438, 509, 565, 640
Lsp1109I GCAGC 2 cut(s) 318, 666
LweI GCATC 3 cut(s) 111, 574, 598
MaeII ACGT 3 cut(s) 96, 159, 219
MaeIII GTNAC 4 cut(s) 11, 220, 491, 528
MalI GATC 5 cut(s) 54, 203, 377, 407, 653
MboI GATC 5 cut(s) 52, 201, 375, 405, 651
MboII GAAGA 2 cut(s) 628, 736
MflI RGATCY 1 cut(s) 405
MhlI GDGCHC 3 cut(s) 167, 435, 514
MlsI TGGCCA 2 cut(s) 423, 446
MluCI AATT 3 cut(s) 439, 461, 544
MluNI TGGCCA 2 cut(s) 423, 446
Mly113I GGCGCC 1 cut(s) 111
MlyI GAGTC 5 cut(s) 19, 187, 257, 266, 335
MmeI TCCRAC 2 cut(s) 291, 552
Mox20I TGGCCA 2 cut(s) 423, 446
MroI TCCGGA 1 cut(s) 337
MroNI GCCGGC 1 cut(s) 383
MscI TGGCCA 2 cut(s) 423, 446
MseI TTAA 1 cut(s) 708
MslI CAYNNNNRTG 1 cut(s) 363
Msp20I TGGCCA 2 cut(s) 423, 446
MspI CCGG 7 cut(s) 68, 137, 338, 384, 515, 539, 563
MspR9I CCNGG 4 cut(s) 43, 69, 101, 236
Mva1269I GAATGC 1 cut(s) 358
MvaI CCWGG 3 cut(s) 43, 101, 236
NaeI GCCGGC 1 cut(s) 385
NarI GGCGCC 1 cut(s) 111
NciI CCSGG 1 cut(s) 69
NdeII GATC 5 cut(s) 52, 201, 375, 405, 651
NgoMIV GCCGGC 1 cut(s) 383
NlaIII CATG 4 cut(s) 193, 368, 477, 686
NlaIV GGNNCC 3 cut(s) 112, 407, 642
NmeAIII GCCGAG 1 cut(s) 139
NmuCI GTSAC 4 cut(s) 11, 220, 491, 528
NspI RCATGY 1 cut(s) 368
PaeI GCATGC 1 cut(s) 368
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PctI GAATGC 1 cut(s) 358
PdiI GCCGGC 1 cut(s) 385
PflMI CCANNNNNTGG 1 cut(s) 442
PfoI TCCNGGA 1 cut(s) 99
PkrI GCNGC 3 cut(s) 284, 333, 681
PleI GAGTC 5 cut(s) 19, 187, 257, 266, 334
PluTI GGCGCC 1 cut(s) 114
PmaCI CACGTG 1 cut(s) 160
PmlI CACGTG 1 cut(s) 160
PpsI GAGTC 5 cut(s) 19, 187, 257, 266, 334
Ppu21I YACGTR 1 cut(s) 160
Psp124BI GAGCTC 1 cut(s) 435
Psp6I CCWGG 3 cut(s) 41, 99, 234
PspCI CACGTG 1 cut(s) 160
PspEI GGTNACC 1 cut(s) 491
PspFI CCCAGC 1 cut(s) 46
PspGI CCWGG 3 cut(s) 41, 99, 234
PspN4I GGNNCC 3 cut(s) 112, 407, 642
PspPI GGNCC 1 cut(s) 517
PsuI RGATCY 1 cut(s) 405
RsaI GTAC 1 cut(s) 472
RsaNI GTAC 1 cut(s) 471
RseI CAYNNNNRTG 1 cut(s) 363
SacI GAGCTC 1 cut(s) 435
SaqAI TTAA 1 cut(s) 708
SatI GCNGC 3 cut(s) 283, 332, 680
Sau3AI GATC 5 cut(s) 52, 201, 375, 405, 651
Sau96I GGNCC 1 cut(s) 517
SchI GAGTC 5 cut(s) 19, 187, 257, 266, 335
ScrFI CCNGG 4 cut(s) 43, 69, 101, 236
SduI GDGCHC 3 cut(s) 167, 435, 514
SfaNI GCATC 3 cut(s) 111, 574, 598
SfcI CTRYAG 1 cut(s) 178
SfoI GGCGCC 1 cut(s) 112
SmiMI CAYNNNNRTG 1 cut(s) 363
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
SphI GCATGC 1 cut(s) 368
Sse9I AATT 3 cut(s) 439, 461, 544
SsiI CCGC 6 cut(s) 36, 283, 638, 659, 665, 698
SspDI GGCGCC 1 cut(s) 110
SstI GAGCTC 1 cut(s) 435
StyD4I CCNGG 4 cut(s) 41, 67, 99, 234
TaiI ACGT 3 cut(s) 99, 162, 222
TaqI TCGA 2 cut(s) 204, 623
TaqII GACCGA 2 cut(s) 501, 510
TasI AATT 3 cut(s) 439, 461, 544
TatI WGTACW 1 cut(s) 470
TauI GCSGC 1 cut(s) 285
Tru1I TTAA 1 cut(s) 708
Tru9I TTAA 1 cut(s) 708
TscAI CASTG 2 cut(s) 254, 510
TseFI GTSAC 4 cut(s) 11, 220, 491, 528
TseI GCWGC 2 cut(s) 331, 679
Tsp45I GTSAC 4 cut(s) 11, 220, 491, 528
TspDTI ATGAA 1 cut(s) 737
TspGWI ACGGA 2 cut(s) 471, 620
TspRI CASTG 2 cut(s) 254, 510
Van91I CCANNNNNTGG 1 cut(s) 442
VneI GTGCAC 1 cut(s) 163
XagI CCTNNNNNAGG 2 cut(s) 104, 233
XapI RAATTY 3 cut(s) 439, 461, 544
XceI RCATGY 1 cut(s) 368
XmiI GTMKAC 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.