MD10G1291000.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010503
Physical Location & Seq
Reverse (-)
37966515 .. 37972151
5637 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1291000.v1.1.491

Sequence Viewer

Length: 1947 bp
ATGAGAAAAGCAAATGGCACATGGGCGTTGCGTGGTTCGGAGCTAAAGCATTTGGCTAGGTCTGTTCATCGTCTTCGTCTTCCTCTTCCGTGGCTGCTGCTTCTGCTCTGCTGCTCGGTGTTTTCAGTCGCTTCGGCCAGACCCCCGCCATTTGCTATGCGCATAAGCTGTGGAGCTCGGGAGAATGTGATTACAAAACCATCCAATACCACATGGTTTAAGGATTTTGATTATTCAGGGGGGATACCTTTTAATGCGACCCACCCGAGTTTTATCACTCCTCCACTTGATACACTTCGCTATTTTCCCCTCTCCTCAGGTCCTGAAAATTGTTACATGATCAATAGAGTTCCCCATGGGCACTATGTAATCAGGATTTTCTTTGGGTCGATTGCACTACCAAACTATGACACCGAACCTTTATTTGATATTTCTGTTGAGGGAACTCAAATTACCTCTTTAAAATCTGGTTGGAGTACCCACGATGATCAAGTATTTTCTGAAGCGCTTGTATATCTGAGAGATGGATCTGTGTCTATCTGCTTCCATAGTACTGGTCATGGGGATCCGGCAATTCTTGCCATTGAAATTCTTCAGATTGATGAAAGAGCATACCAGTTTAGCCAGCAAGTATCTGAAGGAATAATCCTTAGAACAGCAAAAAGATTGAGCTGTGGTGTTGAAGACGCGAAGTTTGGTGTGGATTACAACGGCGATCAATGGGGTGGGGATAGATTTTGGAATTATATAATGACTTTTGGTCAGATTGCTGATACACGCAGATCCACTCAGGGAACCATTAAGCAGGCTTCAAGTCCACCAAACTTCTACCCAGCTGCTCTTTATAAACAAGCAATTGTGAGTATTGATAGTCAGCTCGACTTAGAATATACCCTGGATGTGGATCCCAACAGAAACTACTCCATTTGGTTGCACTTTGCTGAGATTGATGCTTCGATCACTGGTGTAGGCCAAAGGGTATTTGATATCCTGATTAACGGTGACACTTTATTTAAAGATGTGGATGTTATCAAATTGAGTGGGGACCGTTATGCTGCTCTTGTACTTAACACAACTGTTGCCGTAAGCGGGAGGACCTTAACCATAACTTTGCACCCTAAACAAGGTGGTGCCATAATCAATGCCATTGAGATCTTTGAGGTTATAATGGCCGAGTCAAAAACTTTATCAGATGAAGTTAATGCGTTACTGTCATTGAAGAAAACATTGGGGCTTCCTGTTCGGTTGGGGTGGAATGGTGATCCATGTGTTCCCCAACAACATCCATGGAGTGGAGCCGATTGCCAGTTTGATAGAACAAGCAACAAATGGGTCATTGACGGACTGGCTCTGGACAACCAAGGGCTGAAGGGATTCTTGCTGAATGACATATCCAAATTACGTCATCTACAAAGCATAAATTTGAGCGGAAATAGCATGCAAGGGCCCATTCCTTCCTCACTTGGAAAGATAACAAGCTTAGAAGTATTGGACTTGTCTTTCAATTCCTTGAACGGGTCAATTCCTGAAACGCTTGGACAGTTGACCTCGTTGCAAACACTTAATGTCAATGGCAATTCTCTGTCCGGAAAGGTCCCAGCTGCACTAGGAGGCAGGCTTTTGCACAGAGCAAGCTTCAATTTCACAGATAACCATGGACTCTGCGGTATACCGGGACTACCTACGTGTGGGCCACACCTCACTGCCGGAGCAAAGATTGGAATTGGGTTTGGTGTTTTGGCTGCCTTCCTACTTCTCGTTATCGGTTCGATATGCTGTTGGAAAAGGCGGCAGAACATCCTTAGAGTACAACAAATTGCAGCAAGAGAAGCTCCGTATGCAAAGGCGAGGACGCATTTGTCACGTGACATCCAGATGAACAGGCAGTACAATCATCACGGCCAATCTAGAACGGCAGCAGAGACTGGGCCTATTTTGCTTACATGA

Protein Analysis

649

Amino Acids

71.16

Weight (kDa)

8.18

Isoelectric Point (pI)

34.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 55 - 388 7.6e-56 Malectin-like domain
Malectin PF11721 223 - 378 1.5e-16 Malectin domain
LRR_14 PF23598 455 - 526 4.7e-07 Leucine-rich repeat region
LRR_8 PF13855 468 - 528 8.2e-08 Leucine rich repeat
LRR_4 PF12799 492 - 529 3e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 846, 1166
AasI GACNNNNNNGTC 1 cut(s) 1858
Acc16I TGCGCA 1 cut(s) 161
AccB1I GGYRCC 1 cut(s) 1130
AccB7I CCANNNNNTGG 1 cut(s) 1292
AccBSI CCGCTC 1 cut(s) 1428
AccI GTMKAC 1 cut(s) 1669
AccII CGCG 1 cut(s) 689
AccIII TCCGGA 1 cut(s) 1586
AciI CCGC 5 cut(s) 146, 1089, 1428, 1665, 1789
AclWI GGATC 7 cut(s) 535, 560, 573, 777, 899, 912, 1256
AcoI YGGCCR 3 cut(s) 135, 1170, 1900
AcsI RAATTY 2 cut(s) 588, 1420
AcuI CTGAAG 4 cut(s) 522, 578, 657, 1388
AcvI CACGTG 1 cut(s) 1865
AfaI GTAC 5 cut(s) 478, 553, 1065, 1809, 1889
AfeI AGCGCT 1 cut(s) 507
AfiI CCNNNNNNNGG 6 cut(s) 901, 1089, 1124, 1292, 1515, 1688
AflIII ACRYGT 1 cut(s) 1685
AgsI TTSAA 7 cut(s) 587, 683, 813, 1219, 1504, 1513, 1639
AhdI GACNNNNNGTC 1 cut(s) 759
AjnI CCWGG 1 cut(s) 894
AjuI GAANNNNNNNTTGG 2 cut(s) 1211, 1243
Alw21I GWGCWC 1 cut(s) 178
Alw26I GTCTC 1 cut(s) 1916
AlwI GGATC 7 cut(s) 535, 560, 573, 777, 899, 912, 1256
AlwNI CAGNNNCTG 2 cut(s) 323, 1925
Ama87I CYCGRG 2 cut(s) 177, 265
Aor13HI TCCGGA 1 cut(s) 1586
Aor51HI AGCGCT 1 cut(s) 507
AoxI GGCC 7 cut(s) 135, 970, 1170, 1445, 1691, 1900, 1928
ApaI GGGCCC 1 cut(s) 1449
ApeKI GCWGC 9 cut(s) 94, 97, 111, 836, 1055, 1601, 1742, 1820, 1916
ApoI RAATTY 2 cut(s) 588, 1420
ArsI GACNNNNNNTTYG 2 cut(s) 677, 709
Asp700I GAANNNNTTC 2 cut(s) 591, 1373
AspLEI GCGC 2 cut(s) 162, 508
AspS9I GGNCC 8 cut(s) 320, 1045, 1095, 1445, 1446, 1594, 1691, 1928
AsuC2I CCSGG 1 cut(s) 1674
AsuHPI GGTGA 2 cut(s) 1013, 1271
AvaI CYCGRG 2 cut(s) 177, 265
AvaII GGWCC 4 cut(s) 320, 1045, 1095, 1594
AxyI CCTNAGG 1 cut(s) 316
BaeGI GKGCMC 2 cut(s) 363, 1449
BamHI GGATCC 2 cut(s) 565, 904
BanI GGYRCC 1 cut(s) 1130
BanII GRGCYC 2 cut(s) 178, 1449
BbrPI CACGTG 1 cut(s) 1865
BbsI GAAGAC 3 cut(s) 65, 71, 690
Bbv12I GWGCWC 1 cut(s) 178
BbvI GCAGC 9 cut(s) 81, 84, 98, 823, 1042, 1588, 1729, 1832, 1928
BccI CCATC 2 cut(s) 208, 518
BceAI ACGGC 4 cut(s) 727, 1067, 1915, 1929
BciT130I CCWGG 1 cut(s) 896
BciVI GTATCC 1 cut(s) 237
BclI TGATCA 2 cut(s) 339, 487
BcnI CCSGG 1 cut(s) 1674
BcoDI GTCTC 1 cut(s) 1916
BfaI CTAG 3 cut(s) 57, 1607, 1908
BfoI RGCGCY 1 cut(s) 509
BfuI GTATCC 1 cut(s) 237
BglII AGATCT 1 cut(s) 1152
BmcAI AGTACT 1 cut(s) 553
Bme1390I CCNGG 2 cut(s) 896, 1674
Bme18I GGWCC 4 cut(s) 320, 1045, 1095, 1594
BmeRI GACNNNNNGTC 1 cut(s) 759
BmeT110I CYCGRG 2 cut(s) 177, 265
BmgT120I GGNCC 8 cut(s) 320, 1045, 1095, 1445, 1446, 1594, 1691, 1928
BmiI GGNNCC 8 cut(s) 567, 796, 906, 1046, 1132, 1297, 1447, 1596
BmrFI CCNGG 2 cut(s) 896, 1674
BmrI ACTGGG 1 cut(s) 1935
BmsI GCATC 1 cut(s) 940
BmuI ACTGGG 1 cut(s) 1935
BpiI GAAGAC 3 cut(s) 65, 71, 690
BpuMI CCSGG 1 cut(s) 1674
BsaAI YACGTR 2 cut(s) 1686, 1865
BsaBI GATNNNNATC 1 cut(s) 903
BsaJI CCNNGG 6 cut(s) 89, 355, 894, 1286, 1360, 1654
BsaWI WCCGGW 1 cut(s) 1586
Bsc4I CCNNNNNNNGG 6 cut(s) 901, 1089, 1124, 1292, 1515, 1688
Bse1I ACTGG 6 cut(s) 559, 616, 967, 1306, 1350, 1930
Bse21I CCTNAGG 1 cut(s) 316
Bse8I GATNNNNATC 1 cut(s) 903
BseAI TCCGGA 1 cut(s) 1586
BseBI CCWGG 1 cut(s) 896
BseDI CCNNGG 6 cut(s) 89, 355, 894, 1286, 1360, 1654
BseGI GGATG 6 cut(s) 200, 904, 1030, 1282, 1797, 1869
BseJI GATNNNNATC 1 cut(s) 903
BseLI CCNNNNNNNGG 6 cut(s) 901, 1089, 1124, 1292, 1515, 1688
BseMII CTCAG 4 cut(s) 330, 509, 803, 933
BseNI ACTGG 6 cut(s) 559, 616, 967, 1306, 1350, 1930
BseRI GAGGAG 2 cut(s) 270, 304
BseSI GKGCMC 2 cut(s) 363, 1449
BseXI GCAGC 9 cut(s) 81, 84, 98, 823, 1042, 1588, 1729, 1832, 1928
BseYI CCCAGC 2 cut(s) 832, 1597
BsgI GTGCAG 1 cut(s) 1587
Bsh1236I CGCG 1 cut(s) 689
BshFI GGCC 7 cut(s) 137, 972, 1172, 1447, 1693, 1902, 1930
BshNI GGYRCC 1 cut(s) 1130
BsiHKAI GWGCWC 1 cut(s) 178
BsiHKCI CYCGRG 2 cut(s) 177, 265
BsiSI CCGG 4 cut(s) 569, 1587, 1673, 1707
BslFI GGGAC 3 cut(s) 1058, 1580, 1689
BslI CCNNNNNNNGG 6 cut(s) 901, 1089, 1124, 1292, 1515, 1688
BsmAI GTCTC 1 cut(s) 1916
BsmFI GGGAC 3 cut(s) 1058, 1580, 1689
BsnI GGCC 7 cut(s) 137, 972, 1172, 1447, 1693, 1902, 1930
BsoBI CYCGRG 2 cut(s) 177, 265
Bsp120I GGGCCC 1 cut(s) 1445
Bsp1286I GDGCHC 3 cut(s) 178, 363, 1449
Bsp13I TCCGGA 1 cut(s) 1586
Bsp19I CCATGG 3 cut(s) 355, 1286, 1654
BspACI CCGC 5 cut(s) 146, 1089, 1428, 1665, 1789
BspANI GGCC 7 cut(s) 137, 972, 1172, 1447, 1693, 1902, 1930
BspCNI CTCAG 4 cut(s) 329, 510, 802, 934
BspEI TCCGGA 1 cut(s) 1586
BspFNI CGCG 1 cut(s) 689
BspLI GGNNCC 8 cut(s) 567, 796, 906, 1046, 1132, 1297, 1447, 1596
BspPI GGATC 7 cut(s) 535, 560, 573, 777, 899, 912, 1256
BspT107I GGYRCC 1 cut(s) 1130
BsrBI CCGCTC 1 cut(s) 1428
BsrI ACTGG 6 cut(s) 559, 616, 967, 1306, 1350, 1930
BssECI CCNNGG 6 cut(s) 89, 355, 894, 1286, 1360, 1654
BssNAI GTATAC 1 cut(s) 1670
BssT1I CCWWGG 4 cut(s) 355, 1286, 1360, 1654
Bst1107I GTATAC 1 cut(s) 1670
Bst2UI CCWGG 1 cut(s) 896
Bst4CI ACNGT 5 cut(s) 1001, 1049, 1078, 1212, 1542
Bst6I CTCTTC 1 cut(s) 90
BstAPI GCANNNNNTGC 1 cut(s) 578
BstBAI YACGTR 2 cut(s) 1686, 1865
BstC8I GCNNGC 5 cut(s) 626, 807, 1439, 1616, 1633
BstDEI CTNAG 8 cut(s) 316, 518, 650, 789, 883, 942, 1480, 1802
BstDSI CCRYGG 4 cut(s) 89, 355, 1286, 1654
BstENI CCTNNNNNAGG 1 cut(s) 1122
BstF5I GGATG 6 cut(s) 200, 904, 1030, 1282, 1797, 1869
BstFNI CGCG 1 cut(s) 689
BstH2I RGCGCY 1 cut(s) 509
BstHHI GCGC 2 cut(s) 162, 508
BstMAI GTCTC 1 cut(s) 1916
BstMWI GCNNNNNNNGC 6 cut(s) 103, 578, 1434, 1829, 1838, 1936
BstNI CCWGG 1 cut(s) 896
BstNSI RCATGY 1 cut(s) 1441
BstSCI CCNGG 2 cut(s) 894, 1672
BstSLI GKGCMC 2 cut(s) 363, 1449
BstUI CGCG 1 cut(s) 689
BstV1I GCAGC 9 cut(s) 81, 84, 98, 823, 1042, 1588, 1729, 1832, 1928
BstV2I GAAGAC 3 cut(s) 65, 71, 690
BstX2I RGATCY 5 cut(s) 527, 565, 782, 904, 1152
BstXI CCANNNNNNTGG 1 cut(s) 554
BstYI RGATCY 5 cut(s) 527, 565, 782, 904, 1152
BstZ17I GTATAC 1 cut(s) 1670
Bsu36I CCTNAGG 1 cut(s) 316
BsuI GTATCC 1 cut(s) 237
BsuRI GGCC 7 cut(s) 137, 972, 1172, 1447, 1693, 1902, 1930
BtgI CCRYGG 4 cut(s) 89, 355, 1286, 1654
BtsCI GGATG 6 cut(s) 200, 904, 1030, 1282, 1797, 1869
BtsI GCAGTG 1 cut(s) 1701
BtsIMutI CAGTG 2 cut(s) 960, 1701
Cac8I GCNNGC 5 cut(s) 626, 807, 1439, 1616, 1633
CaiI CAGNNNCTG 2 cut(s) 323, 1925
CfoI GCGC 2 cut(s) 162, 508
Cfr13I GGNCC 8 cut(s) 320, 1045, 1095, 1445, 1446, 1594, 1691, 1928
CseI GACGC 2 cut(s) 695, 1861
Csp6I GTAC 5 cut(s) 477, 552, 1064, 1808, 1888
CspCI CAANNNNNGTGG 2 cut(s) 1021, 1056
CviQI GTAC 5 cut(s) 477, 552, 1064, 1808, 1888
DdeI CTNAG 8 cut(s) 316, 518, 650, 789, 883, 942, 1480, 1802
DraI TTTAAA 2 cut(s) 462, 1015
DrdI GACNNNNNNGTC 1 cut(s) 1858
DriI GACNNNNNGTC 1 cut(s) 759
DseDI GACNNNNNNGTC 1 cut(s) 1858
EaeI YGGCCR 3 cut(s) 135, 1170, 1900
Eam1104I CTCTTC 1 cut(s) 90
Eam1105I GACNNNNNGTC 1 cut(s) 759
EarI CTCTTC 1 cut(s) 90
Ecl136II GAGCTC 1 cut(s) 176
Eco130I CCWWGG 4 cut(s) 355, 1286, 1360, 1654
Eco24I GRGCYC 2 cut(s) 178, 1449
Eco32I GATATC 1 cut(s) 988
Eco47I GGWCC 4 cut(s) 320, 1045, 1095, 1594
Eco47III AGCGCT 1 cut(s) 507
Eco53kI GAGCTC 1 cut(s) 176
Eco57I CTGAAG 4 cut(s) 522, 578, 657, 1388
Eco72I CACGTG 1 cut(s) 1865
Eco81I CCTNAGG 1 cut(s) 316
Eco88I CYCGRG 2 cut(s) 177, 265
EcoICRI GAGCTC 1 cut(s) 176
EcoNI CCTNNNNNAGG 1 cut(s) 1122
EcoO109I RGGNCCY 4 cut(s) 320, 1095, 1445, 1594
EcoRII CCWGG 1 cut(s) 894
EcoRV GATATC 1 cut(s) 988
EcoT14I CCWWGG 4 cut(s) 355, 1286, 1360, 1654
EcoT38I GRGCYC 2 cut(s) 178, 1449
ErhI CCWWGG 4 cut(s) 355, 1286, 1360, 1654
FalI AAGNNNNNCTT 2 cut(s) 1361, 1393
FaqI GGGAC 3 cut(s) 1058, 1580, 1689
FauI CCCGC 2 cut(s) 153, 1082
FbaI TGATCA 2 cut(s) 339, 487
FblI GTMKAC 1 cut(s) 1669
FokI GGATG 6 cut(s) 187, 911, 1037, 1269, 1784, 1856
FriOI GRGCYC 2 cut(s) 178, 1449
FspAI RTGCGCAY 1 cut(s) 161
FspBI CTAG 3 cut(s) 57, 1607, 1908
FspI TGCGCA 1 cut(s) 161
GlaI GCGC 2 cut(s) 161, 507
GsaI CCCAGC 2 cut(s) 836, 1601
HaeII RGCGCY 1 cut(s) 509
HaeIII GGCC 7 cut(s) 137, 972, 1172, 1447, 1693, 1902, 1930
HapII CCGG 4 cut(s) 569, 1587, 1673, 1707
HgaI GACGC 2 cut(s) 695, 1861
HhaI GCGC 2 cut(s) 162, 508
Hin6I GCGC 2 cut(s) 160, 506
HinP1I GCGC 2 cut(s) 160, 506
HincII GTYRAC 1 cut(s) 1545
HindII GTYRAC 1 cut(s) 1545
HindIII AAGCTT 2 cut(s) 1477, 1633
HinfI GANTC 3 cut(s) 1175, 1374, 1659
HpaII CCGG 4 cut(s) 569, 1587, 1673, 1707
HphI GGTGA 2 cut(s) 1013, 1271
Hpy166II GTNNAC 3 cut(s) 818, 1545, 1670
Hpy188I TCNGA 7 cut(s) 40, 502, 519, 597, 637, 765, 1192
Hpy188III TCNNGA 9 cut(s) 179, 323, 373, 991, 1352, 1526, 1587, 1873, 1908
Hpy8I GTNNAC 3 cut(s) 818, 1545, 1670
HpyAV CCTTC 4 cut(s) 632, 1363, 1464, 1756
HpyCH4III ACNGT 5 cut(s) 1001, 1049, 1078, 1212, 1542
HpyCH4IV ACGT 3 cut(s) 1402, 1685, 1864
HpyCH4V TGCA 9 cut(s) 395, 934, 1114, 1441, 1555, 1604, 1624, 1820, 1841
HpyF10VI GCNNNNNNNGC 6 cut(s) 103, 578, 1434, 1829, 1838, 1936
HpyF3I CTNAG 8 cut(s) 316, 518, 650, 789, 883, 942, 1480, 1802
HpySE526I ACGT 3 cut(s) 1402, 1685, 1864
HspAI GCGC 2 cut(s) 160, 506
Kpn2I TCCGGA 1 cut(s) 1586
Ksp22I TGATCA 2 cut(s) 339, 487
LmnI GCTCC 5 cut(s) 40, 173, 1295, 1709, 1837
Lsp1109I GCAGC 9 cut(s) 81, 84, 98, 823, 1042, 1588, 1729, 1832, 1928
LweI GCATC 1 cut(s) 940
MaeI CTAG 3 cut(s) 57, 1607, 1908
MaeII ACGT 3 cut(s) 1402, 1685, 1864
MaeIII GTNAC 5 cut(s) 332, 1001, 1206, 1860, 1865
MbiI CCGCTC 1 cut(s) 1428
MboII GAAGA 6 cut(s) 65, 71, 77, 584, 695, 1231
MfeI CAATTG 1 cut(s) 855
MflI RGATCY 5 cut(s) 527, 565, 782, 904, 1152
MhlI GDGCHC 3 cut(s) 178, 363, 1449
MlyI GAGTC 2 cut(s) 1184, 1653
MmeI TCCRAC 2 cut(s) 452, 1760
MroI TCCGGA 1 cut(s) 1586
MroXI GAANNNNTTC 2 cut(s) 591, 1373
MslI CAYNNNNRTG 1 cut(s) 1874
MspA1I CMGCKG 2 cut(s) 836, 1601
MspI CCGG 4 cut(s) 569, 1587, 1673, 1707
MspR9I CCNGG 2 cut(s) 896, 1674
MunI CAATTG 1 cut(s) 855
MvaI CCWGG 1 cut(s) 896
MvnI CGCG 1 cut(s) 689
MwoI GCNNNNNNNGC 6 cut(s) 103, 578, 1434, 1829, 1838, 1936
NciI CCSGG 1 cut(s) 1674
NcoI CCATGG 3 cut(s) 355, 1286, 1654
NlaIV GGNNCC 8 cut(s) 567, 796, 906, 1046, 1132, 1297, 1447, 1596
NmeAIII GCCGAG 1 cut(s) 1198
NmuCI GTSAC 3 cut(s) 1001, 1860, 1865
NsbI TGCGCA 1 cut(s) 161
NspI RCATGY 1 cut(s) 1441
PaeI GCATGC 1 cut(s) 1441
PdmI GAANNNNTTC 2 cut(s) 591, 1373
PfeI GAWTC 1 cut(s) 1374
PflMI CCANNNNNTGG 1 cut(s) 1292
PleI GAGTC 2 cut(s) 1183, 1653
PmaCI CACGTG 1 cut(s) 1865
PmlI CACGTG 1 cut(s) 1865
PpsI GAGTC 2 cut(s) 1183, 1653
Ppu21I YACGTR 2 cut(s) 1686, 1865
PpuMI RGGWCCY 3 cut(s) 320, 1095, 1594
PsiI TTATAA 2 cut(s) 846, 1166
Psp124BI GAGCTC 1 cut(s) 178
Psp5II RGGWCCY 3 cut(s) 320, 1095, 1594
Psp6I CCWGG 1 cut(s) 894
PspCI CACGTG 1 cut(s) 1865
PspFI CCCAGC 2 cut(s) 832, 1597
PspGI CCWGG 1 cut(s) 894
PspN4I GGNNCC 8 cut(s) 567, 796, 906, 1046, 1132, 1297, 1447, 1596
PspOMI GGGCCC 1 cut(s) 1445
PspPI GGNCC 8 cut(s) 320, 1045, 1095, 1445, 1446, 1594, 1691, 1928
PspPPI RGGWCCY 3 cut(s) 320, 1095, 1594
PsrI GAACNNNNNNTAC 4 cut(s) 436, 468, 1871, 1903
PstNI CAGNNNCTG 2 cut(s) 323, 1925
PsuI RGATCY 5 cut(s) 527, 565, 782, 904, 1152
PvuII CAGCTG 2 cut(s) 836, 1601
RsaI GTAC 5 cut(s) 478, 553, 1065, 1809, 1889
RsaNI GTAC 5 cut(s) 477, 552, 1064, 1808, 1888
RseI CAYNNNNRTG 1 cut(s) 1874
SacI GAGCTC 1 cut(s) 178
Sau96I GGNCC 8 cut(s) 320, 1045, 1095, 1445, 1446, 1594, 1691, 1928
ScaI AGTACT 1 cut(s) 553
SchI GAGTC 2 cut(s) 1184, 1653
ScrFI CCNGG 2 cut(s) 896, 1674
SduI GDGCHC 3 cut(s) 178, 363, 1449
SfaNI GCATC 1 cut(s) 940
SinI GGWCC 4 cut(s) 320, 1045, 1095, 1594
SmiMI CAYNNNNRTG 1 cut(s) 1874
SphI GCATGC 1 cut(s) 1441
SsiI CCGC 5 cut(s) 146, 1089, 1428, 1665, 1789
SspMI CTAG 3 cut(s) 57, 1607, 1908
SstI GAGCTC 1 cut(s) 178
StyD4I CCNGG 2 cut(s) 894, 1672
StyI CCWWGG 4 cut(s) 355, 1286, 1360, 1654
TaaI ACNGT 5 cut(s) 1001, 1049, 1078, 1212, 1542
TaiI ACGT 3 cut(s) 1405, 1688, 1867
TaqI TCGA 4 cut(s) 389, 879, 956, 1769
TatI WGTACW 4 cut(s) 551, 1063, 1807, 1887
TauI GCSGC 1 cut(s) 1792
TfiI GAWTC 1 cut(s) 1374
TscAI CASTG 2 cut(s) 967, 1708
TseFI GTSAC 3 cut(s) 1001, 1860, 1865
TseI GCWGC 9 cut(s) 94, 97, 111, 836, 1055, 1601, 1742, 1820, 1916
Tsp45I GTSAC 3 cut(s) 1001, 1860, 1865
TspDTI ATGAA 4 cut(s) 56, 618, 1209, 1892
TspGWI ACGGA 3 cut(s) 78, 1356, 1824
TspRI CASTG 2 cut(s) 967, 1708
Van91I CCANNNNNTGG 1 cut(s) 1292
VpaK11BI GGWCC 4 cut(s) 320, 1045, 1095, 1594
XagI CCTNNNNNAGG 1 cut(s) 1122
XapI RAATTY 2 cut(s) 588, 1420
XbaI TCTAGA 1 cut(s) 1907
XceI RCATGY 1 cut(s) 1441
XmiI GTMKAC 1 cut(s) 1669
XmnI GAANNNNTTC 2 cut(s) 591, 1373
XspI CTAG 3 cut(s) 57, 1607, 1908
ZrmI AGTACT 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.