MD10G1301600.v1.1

Galactose binding lectin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016929
Physical Location & Seq
Reverse (-)
38866098 .. 38871792
5695 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1301600.v1.1.491

Sequence Viewer

Length: 2553 bp
ATGATGGGGAGCAAGAATTGGGTAGCATTAGTTCTGTTTGTCCTTTTAGTTTTTGAAGGTTATTTCACTGAAACTGTGGTTTCTGCCACCGTGACATATGACCACAGAGCACTGGTTATTGATGGGAAGCGGCGGATTTTGCAGTCGGGCTCTTTTCATTACCCTCGAACCACTCCGGAGGTGTGGCCGGAAATTATTAAGAAAGCGAAGGAAGGGGGAATAGACGTGATTGAGACTTACGTTTTTTGGAACTACCATGAGCCAGTAAAAGGCCAGTACTACTTTGAAGGCAGATTTGATTTGGTGAAATTTGTGAAGACAGTCCAAGAATCAGGCCTTCTAGTTCATTTGAGGATTGGCCCGTATTCTTGTGCTGAATGGAACTACGGGGGATTTCCAATTTGGTTACACTTTATTCCCGGAATTCAGTTTCGCACAACAAATGCACCTTTTAAGAAGGAAATGAAGATATTTCTTGCAAAAATTGTTGAAATGATGAAAATGGAGAATCTCTTTGCATCACAAGGAGGCCCAATCATTCTTACTCAGGTTGAAAATGAATTTGGAAATGTTGAAGGGTCCTATGGGGTTGGCGGAGAATTATATGTCAAATGGGCTGCAGAAACTGCTGTTAGTCTCAATACATCTGTACCTTGGGTGATGTGTGTGCAAGATGATGCCCCGGATCCAGTTATAAACACATGCAATGGATTTTATTGCGATGAGTTTACTCCAAATTCCCCATCCAAACCGAAGATGTGGACAGAGAATTACAGTGGATGGTTCCTTGGATTTGGCAATCCAATCCCCTTTCGACCTGTTGAGGACCTTGCTTTTGCTGTTGCACGATTTTTCGAAACAGGTGGCACTTTTCAAAACTATTACATGTATTTTGGCGGTACCAACTTTGGGCGAACTGCTGGAGGCCCTTTGGTTGCAACGAGCTATGATTATGATGCTCCTATAGATGAATATGGTTTTCTTAGACAGCCAAAATGGGGACATCTGCGTGACTTGCACCTGGCAATAAAACAGTGTGAAGAATATATGATCAGCTCTGATCCGACACAAATGAAACTTGGTACTAATTTAGAGGCACATATCTACCATAAAACATCCAATGAGTGTGCGGCATTTCTTGCTAATTATGACAGTAGTTCAGATGCAAATGTCACTTTTAATGGAAATATCTATTTCCTTCCAGCTTGGTCTGTGAGCATCCTTCCGGATTGTAAGAATGTTATATTTAACACCGCTAAGGTTGTCTCACAAAGAAATATTGGACATTCTCCTTTCGCCCTCACACCCGGTGTACATGATTTTATACTGGAACCAGCTTCATGGAGTTGGTATAAAGAGAGAGTTGGTACTTGGAGTAACAATTCATTTACTGACTCAGGCTTACTGGAGCAGATAAGTACTACGAAAGATGTTAGTGATTATTTATGGTATACAATAAGCATTAATGTGAATGAAAACATAATAACCCGTCAAGAGGAAGAACTTTTTTTACACATCGAGAGTCTGGGACATGCAGCCCTAGTTTTCGTAAATAAAAGACTTGTGGGATTTGGATATGGTAACCATGAAGATGCAAGTTTCATCGTTGATGAGAAGATTACTCTAAACCACGGAAACAATACAATAGATTTGTTGAGTATGATGATTGGTGTACAGAATTACGGGCCGTGGTTCGATGTTGCAGGAGCAGGAATCTTTTCTGTTGTTCTTAGAGACTTGAAGAACAGAACAAATGATTTTTCTTTTGAAGAATGGACCTACCAGGTGGGACTTGAGGGGGAATACCTTGGATTGGATAACATAAGCCTTGCAAACAGTTCACTGTGGACTACAGGGGCTGCTCCACCACTTAATCAGAGTTTGATATGGTACAAGGTTTCATTCCTTGCTCCTGAAGGCAAGGGTCCTTTGGCGTTAAATCTTGCTAGCATGGGAAAGGGACAAGCGTGGGTTAACGGGCAGAGTGTAGGGAGATATTGGCAAGCCTACCTCTCGCCATCAACTGGTTGCGTAAATGGCAGTGATTGTGACTACAGAGGAGCCTATAACGCATCTAAATGTCTAAGGAACTGTGGTCAACCGGCTCAAACTCTGTATCATATTCCCCGTACATGGGTACATACCGGAGATAACCTTCTAGTTCTCCACGAAGAGCTTGGTGGTGACCCTTCAAAGATTTCTTTGAGGACAAAAACTGGCCAAGAAATATGTGCACATGTATCCGAGACTGATCCTCCGCCTGCCAGTTCTTGGAAACCAAACTCGGAACTTATATCTCAAAACCCTGAAGTTCAACTGACCTGCGAACGAGGATGGAGTATCACTTCAATCAATTTTGCTAGCTTTGGAACTCCTATTGGAAATTGTGGCACATTCGCTTCGGGAGCTTGCAGTGCTGACATCTTATCAATCGTTCAGCAGGCTTGCCTTGGCCAAGAAGGATGTTCAATCCCTATCTCCGCTGCAACCTTGGGCGATCCTTGCCCTGGAGTGCTTAAGAGTTTAGCTGTCGAAGCTATTTGCAGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gal_Lectin Glyco_hydro_35

Protein Analysis

851

Amino Acids

94.39

Weight (kDa)

5.15

Isoelectric Point (pI)

36.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 38 - 341 2.4e-114 Glycosyl hydrolases family 35
GHD PF17834 365 - 421 1.2e-26 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 628 - 718 2.5e-21 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 773 - 848 2.7e-16 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016929)

Species Orthologous Gene IDs
malus_domestica MD10G1301600.v1.1
prunus_persica Prupe.4G038600_v2.0.a1
pyrus_communis pycom10g25440
rosa_chinensis RchiOBHm_Chr5g0006691
rosa_laevigata RLG00000031417 RLG00000031511
rosa_multiflora Rmu_sc0017347.1_g000002 Rmu_sc0018387.1_g000009
rosa_roxburghii Rroxscaffold_1G00069330
rosa_rugosa Rorug04G0423700
rosa_samantha Rh5AG051500 Rh5CG059000
rosa_wichuraiana Rw5G005080 Rw5G005300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 695
Acc36I ACCTGC 1 cut(s) 2330
Acc65I GGTACC 1 cut(s) 899
AccB1I GGYRCC 1 cut(s) 899
AccB7I CCANNNNNTGG 2 cut(s) 2024, 2271
AccI GTMKAC 1 cut(s) 1451
AccIII TCCGGA 2 cut(s) 175, 1225
AciI CCGC 8 cut(s) 130, 133, 594, 897, 1130, 1254, 2258, 2481
AclWI GGATC 5 cut(s) 680, 693, 1055, 2246, 2492
AcoI YGGCCR 3 cut(s) 185, 2218, 2452
AcsI RAATTY 4 cut(s) 308, 423, 560, 736
AcuI CTGAAG 2 cut(s) 1935, 2328
AdeI CACNNNGTG 1 cut(s) 1310
AflII CTTAAG 1 cut(s) 2516
AflIII ACRYGT 2 cut(s) 885, 2236
AjiI CACGTC 1 cut(s) 226
AjnI CCWGG 3 cut(s) 1020, 1782, 2506
AjuI GAANNNNNNNTTGG 2 cut(s) 546, 578
AluBI AGCT 9 cut(s) 945, 1056, 1205, 1337, 2176, 2364, 2408, 2528, 2537
AluI AGCT 9 cut(s) 945, 1056, 1205, 1337, 2176, 2364, 2408, 2528, 2537
Alw21I GWGCWC 2 cut(s) 112, 2236
Alw26I GTCTC 5 cut(s) 227, 641, 1270, 1728, 2240
Alw44I GTGCAC 1 cut(s) 2232
AlwI GGATC 5 cut(s) 680, 693, 1055, 2246, 2492
AlwNI CAGNNNCTG 2 cut(s) 626, 1859
Aor13HI TCCGGA 2 cut(s) 175, 1225
AoxI GGCC 9 cut(s) 185, 271, 334, 358, 529, 925, 1685, 2218, 2452
ApaLI GTGCAC 1 cut(s) 2232
ApeKI GCWGC 4 cut(s) 617, 1535, 1859, 2483
ApoI RAATTY 4 cut(s) 308, 423, 560, 736
ArsI GACNNNNNNTTYG 2 cut(s) 818, 850
AseI ATTAAT 1 cut(s) 1464
Asp700I GAANNNNTTC 1 cut(s) 1717
Asp718I GGTACC 1 cut(s) 899
AspS9I GGNCC 8 cut(s) 359, 530, 579, 826, 926, 1685, 1776, 1925
AsuC2I CCSGG 3 cut(s) 420, 683, 1308
AsuHPI GGTGA 3 cut(s) 316, 670, 2195
AsuII TTCGAA 1 cut(s) 855
AsuNHI GCTAGC 2 cut(s) 1946, 2360
AvaII GGWCC 4 cut(s) 579, 826, 1776, 1925
BaeGI GKGCMC 1 cut(s) 2236
BaeI ACNNNNGTAYC 2 cut(s) 633, 666
BalI TGGCCA 2 cut(s) 2220, 2454
BamHI GGATCC 1 cut(s) 685
BanI GGYRCC 1 cut(s) 899
BanII GRGCYC 1 cut(s) 152
BbsI GAAGAC 1 cut(s) 323
Bbv12I GWGCWC 2 cut(s) 112, 2236
BbvI GCAGC 4 cut(s) 604, 1547, 1846, 2470
BccI CCATC 5 cut(s) 116, 751, 774, 2026, 2328
BceAI ACGGC 1 cut(s) 1672
BciT130I CCWGG 3 cut(s) 1022, 1784, 2508
BciVI GTATCC 1 cut(s) 2251
BclI TGATCA 1 cut(s) 1050
BcnI CCSGG 3 cut(s) 420, 683, 1308
BcoDI GTCTC 5 cut(s) 227, 641, 1270, 1728, 2240
BfaI CTAG 5 cut(s) 341, 1541, 1947, 2159, 2361
BfmI CTRYAG 4 cut(s) 618, 963, 1851, 2053
BfrI CTTAAG 1 cut(s) 2516
BfuAI ACCTGC 1 cut(s) 2330
BfuI GTATCC 1 cut(s) 2251
BisI GCNGC 6 cut(s) 131, 618, 1131, 1536, 1860, 2484
BlsI GCNGC 6 cut(s) 132, 619, 1132, 1537, 1861, 2485
BmcAI AGTACT 2 cut(s) 278, 1420
Bme1390I CCNGG 6 cut(s) 420, 683, 1022, 1308, 1784, 2508
Bme18I GGWCC 4 cut(s) 579, 826, 1776, 1925
BmgBI CACGTC 1 cut(s) 226
BmgT120I GGNCC 8 cut(s) 359, 530, 579, 826, 926, 1685, 1776, 1925
BmiI GGNNCC 7 cut(s) 580, 687, 785, 901, 1332, 1926, 2062
BmrFI CCNGG 6 cut(s) 420, 683, 1022, 1308, 1784, 2508
BmsI GCATC 7 cut(s) 527, 667, 946, 1153, 1227, 1582, 2081
BmtI GCTAGC 2 cut(s) 1950, 2364
BpiI GAAGAC 1 cut(s) 323
BpmI CTGGAG 3 cut(s) 942, 1427, 2529
Bpu10I CCTNAGC 1 cut(s) 1257
Bpu14I TTCGAA 1 cut(s) 855
BpuEI CTTGAG 1 cut(s) 1814
BpuMI CCSGG 3 cut(s) 420, 683, 1308
BsaJI CCNNGG 9 cut(s) 653, 681, 787, 1630, 1688, 1807, 2449, 2490, 2506
BsaWI WCCGGW 3 cut(s) 175, 1225, 2144
BsaXI ACNNNNNCTCC 2 cut(s) 2239, 2269
Bse118I RCCGGY 1 cut(s) 2101
Bse1I ACTGG 9 cut(s) 117, 263, 274, 689, 1332, 1410, 2029, 2221, 2265
Bse3DI GCAATG 1 cut(s) 712
BseAI TCCGGA 2 cut(s) 175, 1225
BseBI CCWGG 3 cut(s) 1022, 1784, 2508
BseDI CCNNGG 9 cut(s) 653, 681, 787, 1630, 1688, 1807, 2449, 2490, 2506
BseGI GGATG 6 cut(s) 743, 785, 1115, 1218, 2339, 2468
BseMI GCAATG 1 cut(s) 712
BseMII CTCAG 2 cut(s) 560, 1410
BseNI ACTGG 9 cut(s) 117, 263, 274, 689, 1332, 1410, 2029, 2221, 2265
BseRI GAGGAG 1 cut(s) 2073
BseSI GKGCMC 1 cut(s) 2236
BseXI GCAGC 4 cut(s) 604, 1547, 1846, 2470
BshFI GGCC 9 cut(s) 187, 273, 336, 360, 531, 927, 1687, 2220, 2454
BshNI GGYRCC 1 cut(s) 899
BsiHKAI GWGCWC 2 cut(s) 112, 2236
BsiSI CCGG 8 cut(s) 176, 188, 420, 683, 1226, 1308, 2102, 2145
BslFI GGGAC 4 cut(s) 1014, 1542, 1803, 1974
BsmAI GTCTC 5 cut(s) 227, 641, 1270, 1728, 2240
BsmFI GGGAC 4 cut(s) 1014, 1542, 1803, 1974
BsnI GGCC 9 cut(s) 187, 273, 336, 360, 531, 927, 1687, 2220, 2454
Bsp119I TTCGAA 1 cut(s) 855
Bsp1286I GDGCHC 3 cut(s) 112, 152, 2236
Bsp13I TCCGGA 2 cut(s) 175, 1225
Bsp1407I TGTACA 2 cut(s) 1312, 1672
Bsp143I GATC 5 cut(s) 685, 1050, 1060, 2251, 2497
BspACI CCGC 8 cut(s) 130, 133, 594, 897, 1130, 1254, 2258, 2481
BspANI GGCC 9 cut(s) 187, 273, 336, 360, 531, 927, 1687, 2220, 2454
BspCNI CTCAG 2 cut(s) 559, 1409
BspEI TCCGGA 2 cut(s) 175, 1225
BspLI GGNNCC 7 cut(s) 580, 687, 785, 901, 1332, 1926, 2062
BspMAI CTGCAG 1 cut(s) 622
BspMI ACCTGC 1 cut(s) 2330
BspOI GCTAGC 2 cut(s) 1950, 2364
BspPI GGATC 5 cut(s) 680, 693, 1055, 2246, 2492
BspQI GCTCTTC 1 cut(s) 2166
BspT104I TTCGAA 1 cut(s) 855
BspT107I GGYRCC 1 cut(s) 899
BspTI CTTAAG 1 cut(s) 2516
BsrDI GCAATG 1 cut(s) 712
BsrFI RCCGGY 1 cut(s) 2101
BsrGI TGTACA 2 cut(s) 1312, 1672
BsrI ACTGG 9 cut(s) 117, 263, 274, 689, 1332, 1410, 2029, 2221, 2265
BssAI RCCGGY 1 cut(s) 2101
BssECI CCNNGG 9 cut(s) 653, 681, 787, 1630, 1688, 1807, 2449, 2490, 2506
BssMI GATC 5 cut(s) 685, 1050, 1060, 2251, 2497
BssNAI GTATAC 1 cut(s) 1452
BssT1I CCWWGG 5 cut(s) 653, 787, 1807, 2449, 2490
Bst1107I GTATAC 1 cut(s) 1452
Bst2UI CCWGG 3 cut(s) 1022, 1784, 2508
Bst4CI ACNGT 9 cut(s) 76, 91, 322, 776, 1035, 1154, 1838, 1845, 2093
Bst6I CTCTTC 1 cut(s) 2166
BstAFI CTTAAG 1 cut(s) 2516
BstAPI GCANNNNNTGC 2 cut(s) 626, 1139
BstAUI TGTACA 2 cut(s) 1312, 1672
BstBI TTCGAA 1 cut(s) 855
BstC8I GCNNGC 7 cut(s) 1948, 2004, 2262, 2362, 2410, 2442, 2446
BstDEI CTNAG 6 cut(s) 546, 983, 1257, 1396, 1730, 2084
BstDSI CCRYGG 2 cut(s) 1630, 1688
BstEII GGTNACC 2 cut(s) 1580, 2183
BstF5I GGATG 6 cut(s) 743, 785, 1115, 1218, 2339, 2468
BstKTI GATC 5 cut(s) 688, 1053, 1063, 2254, 2500
BstMAI GTCTC 5 cut(s) 227, 641, 1270, 1728, 2240
BstMBI GATC 5 cut(s) 685, 1050, 1060, 2251, 2497
BstNI CCWGG 3 cut(s) 1022, 1784, 2508
BstNSI RCATGY 4 cut(s) 705, 889, 1535, 2240
BstPI GGTNACC 2 cut(s) 1580, 2183
BstSCI CCNGG 6 cut(s) 418, 681, 1020, 1306, 1782, 2506
BstSFI CTRYAG 4 cut(s) 618, 963, 1851, 2053
BstSLI GKGCMC 1 cut(s) 2236
BstV1I GCAGC 4 cut(s) 604, 1547, 1846, 2470
BstV2I GAAGAC 1 cut(s) 323
BstX2I RGATCY 1 cut(s) 685
BstXI CCANNNNNNTGG 1 cut(s) 1341
BstYI RGATCY 1 cut(s) 685
BstZ17I GTATAC 1 cut(s) 1452
BsuI GTATCC 1 cut(s) 2251
BsuRI GGCC 9 cut(s) 187, 273, 336, 360, 531, 927, 1687, 2220, 2454
BtgI CCRYGG 2 cut(s) 1630, 1688
BtgZI GCGATG 1 cut(s) 735
BtrI CACGTC 1 cut(s) 226
BtsCI GGATG 6 cut(s) 743, 785, 1115, 1218, 2339, 2468
BtsI GCAGTG 3 cut(s) 2047, 2419, 2551
BtsIMutI CAGTG 8 cut(s) 66, 110, 781, 1040, 1841, 2047, 2419, 2551
BveI ACCTGC 1 cut(s) 2330
Cac8I GCNNGC 7 cut(s) 1948, 2004, 2262, 2362, 2410, 2442, 2446
CaiI CAGNNNCTG 2 cut(s) 626, 1859
Cfr10I RCCGGY 1 cut(s) 2101
Cfr13I GGNCC 8 cut(s) 359, 530, 579, 826, 926, 1685, 1776, 1925
CsiI ACCWGGT 1 cut(s) 1782
DdeI CTNAG 6 cut(s) 546, 983, 1257, 1396, 1730, 2084
DpnI GATC 5 cut(s) 687, 1052, 1062, 2253, 2499
DpnII GATC 5 cut(s) 685, 1050, 1060, 2251, 2497
DraIII CACNNNGTG 1 cut(s) 1310
EaeI YGGCCR 3 cut(s) 185, 2218, 2452
Eam1104I CTCTTC 1 cut(s) 2166
EarI CTCTTC 1 cut(s) 2166
EciI GGCGGA 3 cut(s) 148, 609, 2247
Eco130I CCWWGG 5 cut(s) 653, 787, 1807, 2449, 2490
Eco147I AGGCCT 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 152
Eco47I GGWCC 4 cut(s) 579, 826, 1776, 1925
Eco57I CTGAAG 2 cut(s) 1935, 2328
Eco91I GGTNACC 2 cut(s) 1580, 2183
EcoO109I RGGNCCY 4 cut(s) 579, 826, 926, 1925
EcoO65I GGTNACC 2 cut(s) 1580, 2183
EcoRI GAATTC 1 cut(s) 423
EcoRII CCWGG 3 cut(s) 1020, 1782, 2506
EcoT14I CCWWGG 5 cut(s) 653, 787, 1807, 2449, 2490
EcoT38I GRGCYC 1 cut(s) 152
ErhI CCWWGG 5 cut(s) 653, 787, 1807, 2449, 2490
FaqI GGGAC 4 cut(s) 1014, 1542, 1803, 1974
FauNDI CATATG 1 cut(s) 97
FbaI TGATCA 1 cut(s) 1050
FblI GTMKAC 1 cut(s) 1451
Fnu4HI GCNGC 6 cut(s) 131, 618, 1131, 1536, 1860, 2484
FokI GGATG 6 cut(s) 730, 792, 1102, 1205, 2346, 2475
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 6 cut(s) 131, 618, 1131, 1536, 1860, 2484
FspBI CTAG 5 cut(s) 341, 1541, 1947, 2159, 2361
GluI GCNGC 6 cut(s) 131, 618, 1131, 1536, 1860, 2484
GsuI CTGGAG 3 cut(s) 942, 1427, 2529
HaeIII GGCC 9 cut(s) 187, 273, 336, 360, 531, 927, 1687, 2220, 2454
HapII CCGG 8 cut(s) 176, 188, 420, 683, 1226, 1308, 2102, 2145
HincII GTYRAC 2 cut(s) 1975, 2099
HindII GTYRAC 2 cut(s) 1975, 2099
HinfI GANTC 5 cut(s) 329, 508, 1394, 1522, 1713
HpaI GTTAAC 1 cut(s) 1975
HpaII CCGG 8 cut(s) 176, 188, 420, 683, 1226, 1308, 2102, 2145
HphI GGTGA 3 cut(s) 316, 670, 2195
Hpy188I TCNGA 6 cut(s) 1060, 1065, 1162, 1878, 2245, 2287
Hpy188III TCNNGA 6 cut(s) 176, 1226, 1493, 1519, 1913, 2403
HpyCH4III ACNGT 9 cut(s) 76, 91, 322, 776, 1035, 1154, 1838, 1845, 2093
HpyCH4IV ACGT 2 cut(s) 225, 240
HpyF3I CTNAG 6 cut(s) 546, 983, 1257, 1396, 1730, 2084
HpySE526I ACGT 2 cut(s) 225, 240
Kpn2I TCCGGA 2 cut(s) 175, 1225
KpnI GGTACC 1 cut(s) 903
Ksp22I TGATCA 1 cut(s) 1050
KspAI GTTAAC 1 cut(s) 1975
Kzo9I GATC 5 cut(s) 685, 1050, 1060, 2251, 2497
LguI GCTCTTC 1 cut(s) 2166
LmnI GCTCC 8 cut(s) 9, 964, 1408, 1706, 1867, 1915, 2060, 2405
Lsp1109I GCAGC 4 cut(s) 604, 1547, 1846, 2470
LweI GCATC 7 cut(s) 527, 667, 946, 1153, 1227, 1582, 2081
MabI ACCWGGT 1 cut(s) 1782
MaeI CTAG 5 cut(s) 341, 1541, 1947, 2159, 2361
MaeII ACGT 2 cut(s) 225, 240
MaeIII GTNAC 8 cut(s) 91, 405, 1010, 1171, 1376, 1580, 2048, 2183
MalI GATC 5 cut(s) 687, 1052, 1062, 2253, 2499
MboI GATC 5 cut(s) 685, 1050, 1060, 2251, 2497
MflI RGATCY 1 cut(s) 685
MhlI GDGCHC 3 cut(s) 112, 152, 2236
MlsI TGGCCA 2 cut(s) 2220, 2454
MluNI TGGCCA 2 cut(s) 2220, 2454
MlyI GAGTC 2 cut(s) 1388, 1531
MmeI TCCRAC 1 cut(s) 1088
Mox20I TGGCCA 2 cut(s) 2220, 2454
MroI TCCGGA 2 cut(s) 175, 1225
MroXI GAANNNNTTC 1 cut(s) 1717
MscI TGGCCA 2 cut(s) 2220, 2454
MseI TTAA 9 cut(s) 198, 453, 1179, 1248, 1464, 1872, 1937, 1974, 2517
MslI CAYNNNNRTG 4 cut(s) 1008, 1466, 1590, 2077
Msp20I TGGCCA 2 cut(s) 2220, 2454
MspA1I CMGCKG 1 cut(s) 2483
MspCI CTTAAG 1 cut(s) 2516
MspI CCGG 8 cut(s) 176, 188, 420, 683, 1226, 1308, 2102, 2145
MspR9I CCNGG 6 cut(s) 420, 683, 1022, 1308, 1784, 2508
MvaI CCWGG 3 cut(s) 1022, 1784, 2508
NciI CCSGG 3 cut(s) 420, 683, 1308
NdeI CATATG 1 cut(s) 97
NdeII GATC 5 cut(s) 685, 1050, 1060, 2251, 2497
NheI GCTAGC 2 cut(s) 1946, 2360
NlaIV GGNNCC 7 cut(s) 580, 687, 785, 901, 1332, 1926, 2062
NmuCI GTSAC 5 cut(s) 91, 1010, 1171, 2048, 2183
NspI RCATGY 4 cut(s) 705, 889, 1535, 2240
NspV TTCGAA 1 cut(s) 855
PceI AGGCCT 1 cut(s) 336
PciI ACATGT 2 cut(s) 885, 2236
PciSI GCTCTTC 1 cut(s) 2166
PdmI GAANNNNTTC 1 cut(s) 1717
PfeI GAWTC 3 cut(s) 329, 508, 1713
PflMI CCANNNNNTGG 2 cut(s) 2024, 2271
PfoI TCCNGGA 1 cut(s) 418
PkrI GCNGC 6 cut(s) 132, 619, 1132, 1537, 1861, 2485
PleI GAGTC 2 cut(s) 1388, 1530
PpsI GAGTC 2 cut(s) 1388, 1530
PpuMI RGGWCCY 3 cut(s) 579, 826, 1925
PscI ACATGT 2 cut(s) 885, 2236
PshBI ATTAAT 1 cut(s) 1464
PsiI TTATAA 1 cut(s) 695
Psp5II RGGWCCY 3 cut(s) 579, 826, 1925
Psp6I CCWGG 3 cut(s) 1020, 1782, 2506
PspEI GGTNACC 2 cut(s) 1580, 2183
PspGI CCWGG 3 cut(s) 1020, 1782, 2506
PspN4I GGNNCC 7 cut(s) 580, 687, 785, 901, 1332, 1926, 2062
PspPI GGNCC 8 cut(s) 359, 530, 579, 826, 926, 1685, 1776, 1925
PspPPI RGGWCCY 3 cut(s) 579, 826, 1925
PsrI GAACNNNNNNTAC 4 cut(s) 15, 47, 1494, 1526
PstI CTGCAG 1 cut(s) 622
PstNI CAGNNNCTG 2 cut(s) 626, 1859
PsuI RGATCY 1 cut(s) 685
RseI CAYNNNNRTG 4 cut(s) 1008, 1466, 1590, 2077
SapI GCTCTTC 1 cut(s) 2166
SaqAI TTAA 9 cut(s) 198, 453, 1179, 1248, 1464, 1872, 1937, 1974, 2517
SatI GCNGC 6 cut(s) 131, 618, 1131, 1536, 1860, 2484
Sau3AI GATC 5 cut(s) 685, 1050, 1060, 2251, 2497
Sau96I GGNCC 8 cut(s) 359, 530, 579, 826, 926, 1685, 1776, 1925
ScaI AGTACT 2 cut(s) 278, 1420
SchI GAGTC 2 cut(s) 1388, 1531
ScrFI CCNGG 6 cut(s) 420, 683, 1022, 1308, 1784, 2508
SduI GDGCHC 3 cut(s) 112, 152, 2236
SexAI ACCWGGT 1 cut(s) 1782
SfaNI GCATC 7 cut(s) 527, 667, 946, 1153, 1227, 1582, 2081
SfcI CTRYAG 4 cut(s) 618, 963, 1851, 2053
SfuI TTCGAA 1 cut(s) 855
SinI GGWCC 4 cut(s) 579, 826, 1776, 1925
SmiMI CAYNNNNRTG 4 cut(s) 1008, 1466, 1590, 2077
SmlI CTYRAG 2 cut(s) 1793, 2516
SmoI CTYRAG 2 cut(s) 1793, 2516
SseBI AGGCCT 1 cut(s) 336
SsiI CCGC 8 cut(s) 130, 133, 594, 897, 1130, 1254, 2258, 2481
SspI AATATT 1 cut(s) 1279
SspMI CTAG 5 cut(s) 341, 1541, 1947, 2159, 2361
StuI AGGCCT 1 cut(s) 336
StyD4I CCNGG 6 cut(s) 418, 681, 1020, 1306, 1782, 2506
StyI CCWWGG 5 cut(s) 653, 787, 1807, 2449, 2490
TaaI ACNGT 9 cut(s) 76, 91, 322, 776, 1035, 1154, 1838, 1845, 2093
TaiI ACGT 2 cut(s) 228, 243
TaqI TCGA 6 cut(s) 166, 814, 855, 1518, 1695, 2532
TatI WGTACW 4 cut(s) 276, 1312, 1418, 1672
TauI GCSGC 2 cut(s) 133, 1133
TfiI GAWTC 3 cut(s) 329, 508, 1713
Tru1I TTAA 9 cut(s) 198, 453, 1179, 1248, 1464, 1872, 1937, 1974, 2517
Tru9I TTAA 9 cut(s) 198, 453, 1179, 1248, 1464, 1872, 1937, 1974, 2517
TscAI CASTG 8 cut(s) 73, 117, 781, 1040, 1848, 2047, 2419, 2551
TseFI GTSAC 5 cut(s) 91, 1010, 1171, 2048, 2183
TseI GCWGC 4 cut(s) 617, 1535, 1859, 2483
Tsp45I GTSAC 5 cut(s) 91, 1010, 1171, 2048, 2183
TspGWI ACGGA 1 cut(s) 1647
TspRI CASTG 8 cut(s) 73, 117, 781, 1040, 1848, 2047, 2419, 2551
Van91I CCANNNNNTGG 2 cut(s) 2024, 2271
Vha464I CTTAAG 1 cut(s) 2516
VneI GTGCAC 1 cut(s) 2232
VpaK11BI GGWCC 4 cut(s) 579, 826, 1776, 1925
VspI ATTAAT 1 cut(s) 1464
XapI RAATTY 4 cut(s) 308, 423, 560, 736
XceI RCATGY 4 cut(s) 705, 889, 1535, 2240
XcmI CCANNNNNNNNNTGG 1 cut(s) 2174
XmiI GTMKAC 1 cut(s) 1451
XmnI GAANNNNTTC 1 cut(s) 1717
XspI CTAG 5 cut(s) 341, 1541, 1947, 2159, 2361
ZrmI AGTACT 2 cut(s) 278, 1420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.