Rorug04G0423700

disease resistance protein At4g27220

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000394
Physical Location & Seq
Forward (+)
58274855 .. 58281016
6162 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0423700.1

Sequence Viewer

Length: 2094 bp
ATGCGAACTTCCCCTGCAAGGTTTAGGAATCAGCAATCCAGTGCACACCATCCTATACTAAATAAACCCAGTCAGGCGACACGTGTATCTAGTGAAGGCACTCGGGTCTTGGGTCGGGCCGAAATTGACCCGCGTCCGCAACTAAAACCCTCCTCCTATAGTCCTATCCAGTTCCAAAACCCTAGAATTCGCTCCCCTCTCTCCACTGCAATTTCGATTTCAGATTTGGGCATGGGGTTTTTGTTTTCGCAGAGGGCTCTGCTTCTAGAGAAAGTGCTTCTCCTCCGCACTGTAGACCACGCATTTCCTCAGGTATTTCGAAATAGAGTTACTTGGCAGGCAAGAAACACCAAGGGAGCAAGGTTGAGAGCCCCATTTTGTGTAGGATTTCCTTTTCGGTTTCCTCTGAAATTGTATACAACAGGGCAGAAGGGGACTCTGGAAAGGACCCGATTATCAGGCTCGGAAGGAGCAACAATGGTGGTGTCCACGCCAACTGTACATGTGAGAGATACCATTGAACTTGATGAAACTGAAGAGAAGATCTTTGAGAGGCTGCTTGGAACGCTTGACCATTATGGACTTAAGACTGAGCTTCGTGTTGCAGGTGGATGGGTCCGTGATAAGCTTCTGGGAAAAGAGTGCAAGGACATCGACATTGCTTTGGAAAATATGATGGGCAGCAAATTTGTTGAGAAGGTGAGAGACTACTTGTTGTCTGTGGGGGAAGAGGTTCAAGGAATCGCTATAATTCCAAGCAATCCTGATCAATCAAAACATTTGGAAACGGCGAGGATGCGCATCTTTAATATATGGGTTGATTTTGTGAACTTACGTTGTGAAGAGTACAGTGATGATAGCCGCATTCCTACTATGCAAAAGTATGGCACACCAGAACAGGATGCACTGAGAAGGGATCTAACAATAAACAGCTTATTTTACAACATCAATACCAAATCCGTTGAAGATTGGACTAATAGAGGAATTGAAGATCTAAAATGTGGGAAAATTGTGACTCCTTTACCACCAAAGGATACATTTATGGAGGATCCGCTACGAGTTCTTCGAGCTATCCGCTTTGCCGCAAGGTTCGGATTCATACTCGATGAACAACTAAAGGAAGCTGCTGCCTGTGATGAAGTGAAAGCTGCTCTATCAGCTAAAATTAGCAGAGAGCGAATTGGAGCTGAAATTGATCTGATGATTTCTGGAAACCAACCTGTCCAAGCTATGACCTATATATGTGACTTGAAGTTATTTTGGGTTGTCTTCAGTCTTCATCCGCAGTGTGAGCCATCAGAAGAATGCGACAGCGATTGTGTCACTTACTTGGATTGTACATGGAATCTTATTCAATTAGTGGGGCACTCTACCTTCAATGACGAACAAAGAAGGCTCTCCTTTTATGCTGCAATGTTCCTTCCACTTAGGAAGACCATGTATAAAGATAGGAAAGCAAAAGATGTTCCTGTCGTGAATTATATTTTCAGAGACTCCCTTAAGCAAAGAGTCAGCGATGCTGAAACTGTTGTTAATTTACACAATGCATTGGAGAAGTTTTTGTCTTTGCTTCCTCACTTTGTACCAAATGGGGATGCAAAACTCGCTGAAGTTGATTTGGGAAGAGAATATGCTGATGTCCCTCTTACTTCGAAAGAATCAAAACTCCGAGTATTAACAGGGTTTCTTTTACGAGAAATTAAAGATTTTTGGCGAGTTGCTTTGCTGATGTCTATACTGTTATATCCCACCGACGTCTTAAACAAGAATTTCAAACCAGAAGATCGCAGAGCTTTGTTTGTAGAAGCTGAAAAGGCCATAAATAATCTAGGTCTAGATAGAGTCTGGGACGGAAAGCCATTACTTAATGGAAAGGAGATCATGGATGCTTTGCAGCTTAAATCTGGTGGACCAGTTGTCAGTAAATGGCAAAGGAAGCTACTGGCATGGCAGTTTGCTCATCCTTCGGGGACTGCAGAGGAATGCCGCGAGTGGCTGAAGGAAACACACTCTTCAAGTAATAGATCTGCTGGATATATGGAGGAATTGAATGGACCCAACAACAAGAAAAGTAAAACTGAAGATTGTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

697

Amino Acids

79.43

Weight (kDa)

8.63

Isoelectric Point (pI)

41.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PolyA_pol PF01743 199 - 337 1.4e-30 Poly A polymerase head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000394)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21580
fragaria_vesca FvH4_3g02190 FvH4_3g02190 FvH4_3g02190 FvH4_3g04320 FvH4_3g04340 FvH4_3g18080 FvH4_3g18080 FvH4_3g18080 FvH4_6g30490 FvH4_6g30490 FvH4_6g30490 FvH4_6g30490
malus_domestica MD05G1217800.v1.1 MD05G1323900.v1.1 MD12G1074500.v1.1 MD14G1066500.v1.1
prunus_persica Prupe.4G038700_v2.0.a1 Prupe.7G059000_v2.0.a1 Prupe.7G059000_v2.0.a1 Prupe.7G059300_v2.0.a1 Prupe.7G059300_v2.0.a1 Prupe.7G059300_v2.0.a1 Prupe.7G059300_v2.0.a1
pyrus_communis pycom05g29820
rosa_chinensis RchiOBHm_Chr1g0318171 RchiOBHm_Chr5g0003241 RchiOBHm_Chr5g0006701 RchiOBHm_Chr5g0006721 RchiOBHm_Chr5g0006731 RchiOBHm_Chr5g0030211 RchiOBHm_Chr5g0030291 RchiOBHm_Chr5g0030331 RchiOBHm_Chr5g0030371 RchiOBHm_Chr5g0030401 RchiOBHm_Chr5g0030471
rosa_laevigata RLG00000025923 RLG00000025924 RLG00000030556 RLG00000031088 RLG00000031336 RLG00000031418 RLG00000031420 RLG00000031421 RLG00000031422 RLG00000031423 RLG00000033220
rosa_multiflora Rmu_sc0001886.1_g000010 Rmu_sc0001886.1_g000011 Rmu_sc0001886.1_g000012 Rmu_sc0010917.1_g000018 Rmu_sc0011424.1_g000013 Rmu_sc0017347.1_g000003 Rmu_sc0017347.1_g000005 Rmu_sc0018387.1_g000006 Rmu_sc0018387.1_g000008
rosa_roxburghii Rroxscaffold_1G00049810 Rroxscaffold_1G00069320 Rroxscaffold_1G00072770 Rroxscaffold_4G00329990 Rroxscaffold_6G00426520
rosa_rugosa Rorug01G0019200 Rorug01G0019300 Rorug01G0019400 Rorug02G0611300 Rorug02G0611400 Rorug04G0401400 Rorug04G0423700 Rorug04G0424000 Rorug05G0118000
rosa_samantha Rh1CG030000 Rh1DG040100 Rh2DG133300 Rh3AG010300 Rh3BG010000 Rh3DG010500 Rh5AG026800 Rh5AG026900 Rh5AG027800 Rh5AG051600 Rh5AG051800 Rh5BG028000 Rh5BG050700 Rh5BG129800 Rh5BG129900 Rh5CG029400 Rh5CG029500 Rh5CG030100 Rh5CG059100 Rh5CG059300 Rh5CG059400 Rh5DG026200 Rh5DG049800 Rh5DG049900 Rh5DG214000
rosa_wichuraiana Rw1G002510 Rw5G002450 Rw5G002640 Rw5G004650 Rw5G005260 Rw5G005270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 596
AatII GACGTC 1 cut(s) 1758
Acc16I TGCGCA 1 cut(s) 800
Acc36I ACCTGC 1 cut(s) 596
AccI GTMKAC 2 cut(s) 294, 416
AccII CGCG 2 cut(s) 133, 1989
AciI CCGC 9 cut(s) 131, 137, 286, 862, 1052, 1075, 1083, 1283, 1987
AclWI GGATC 3 cut(s) 924, 1043, 1056
AcsI RAATTY 3 cut(s) 186, 686, 1768
AcuI CTGAAG 4 cut(s) 555, 1255, 1629, 2018
AcvI CACGTG 1 cut(s) 83
AcyI GRCGYC 1 cut(s) 1755
AfaI GTAC 4 cut(s) 501, 848, 1339, 1584
AfiI CCNNNNNNNGG 1 cut(s) 18
AflII CTTAAG 2 cut(s) 584, 1499
AflIII ACRYGT 3 cut(s) 80, 82, 502
AhdI GACNNNNNGTC 1 cut(s) 1916
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 2 cut(s) 699, 1485
Alw44I GTGCAC 1 cut(s) 42
AlwI GGATC 3 cut(s) 924, 1043, 1056
Ama87I CYCGRG 1 cut(s) 102
AoxI GGCC 2 cut(s) 117, 1815
ApaLI GTGCAC 1 cut(s) 42
ApeKI GCWGC 7 cut(s) 556, 681, 1124, 1127, 1148, 1409, 1894
ApoI RAATTY 3 cut(s) 186, 686, 1768
Asp700I GAANNNNTTC 1 cut(s) 732
AspLEI GCGC 1 cut(s) 801
AspS9I GGNCC 5 cut(s) 117, 447, 616, 1910, 2054
AsuHPI GGTGA 1 cut(s) 712
AsuII TTCGAA 2 cut(s) 319, 1652
AvaI CYCGRG 1 cut(s) 102
AvaII GGWCC 4 cut(s) 447, 616, 1910, 2054
AxyI CCTNAGG 1 cut(s) 309
BaeGI GKGCMC 2 cut(s) 46, 1368
BaeI ACNNNNGTAYC 2 cut(s) 69, 102
BamHI GGATCC 1 cut(s) 1048
BanII GRGCYC 2 cut(s) 259, 373
BbrPI CACGTG 1 cut(s) 83
BbsI GAAGAC 3 cut(s) 1261, 1268, 1439
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 7 cut(s) 543, 693, 1111, 1114, 1135, 1396, 1906
BccI CCATC 4 cut(s) 57, 606, 670, 1303
BceAI ACGGC 1 cut(s) 804
BcgI CGANNNNNNTGC 2 cut(s) 634, 668
BciVI GTATCC 1 cut(s) 1027
BclI TGATCA 1 cut(s) 766
BcoDI GTCTC 2 cut(s) 699, 1485
BfaI CTAG 5 cut(s) 90, 183, 266, 1829, 1835
BfmI CTRYAG 3 cut(s) 157, 291, 1974
BfrI CTTAAG 2 cut(s) 584, 1499
BfuAI ACCTGC 1 cut(s) 596
BfuI GTATCC 1 cut(s) 1027
BglII AGATCT 3 cut(s) 543, 991, 2024
Bme18I GGWCC 4 cut(s) 447, 616, 1910, 2054
BmeRI GACNNNNNGTC 1 cut(s) 1916
BmeT110I CYCGRG 1 cut(s) 102
BmgT120I GGNCC 5 cut(s) 117, 447, 616, 1910, 2054
BmiI GGNNCC 4 cut(s) 449, 617, 1050, 2056
BmrI ACTGGG 1 cut(s) 63
BmsI GCATC 6 cut(s) 786, 810, 892, 1507, 1585, 1876
BmuI ACTGGG 1 cut(s) 63
BoxI GACNNNNGTC 1 cut(s) 132
BpiI GAAGAC 3 cut(s) 1261, 1268, 1439
Bpu14I TTCGAA 2 cut(s) 319, 1652
BsaAI YACGTR 1 cut(s) 83
BsaBI GATNNNNATC 1 cut(s) 800
BsaHI GRCGYC 1 cut(s) 1755
BsaJI CCNNGG 1 cut(s) 351
Bsc4I CCNNNNNNNGG 1 cut(s) 18
Bse1I ACTGG 5 cut(s) 39, 69, 169, 1913, 1947
Bse21I CCTNAGG 1 cut(s) 309
Bse3DI GCAATG 2 cut(s) 657, 1419
Bse8I GATNNNNATC 1 cut(s) 800
BseDI CCNNGG 1 cut(s) 351
BseGI GGATG 8 cut(s) 49, 617, 801, 907, 1279, 1600, 1891, 1960
BseJI GATNNNNATC 1 cut(s) 800
BseLI CCNNNNNNNGG 1 cut(s) 18
BseMI GCAATG 2 cut(s) 657, 1419
BseMII CTCAG 3 cut(s) 323, 582, 899
BseNI ACTGG 5 cut(s) 39, 69, 169, 1913, 1947
BseRI GAGGAG 2 cut(s) 142, 272
BseSI GKGCMC 2 cut(s) 46, 1368
BseXI GCAGC 7 cut(s) 543, 693, 1111, 1114, 1135, 1396, 1906
Bsh1236I CGCG 2 cut(s) 133, 1989
BshFI GGCC 2 cut(s) 119, 1817
BsiHKAI GWGCWC 1 cut(s) 46
BsiHKCI CYCGRG 1 cut(s) 102
BslFI GGGAC 4 cut(s) 448, 1625, 1862, 1984
BslI CCNNNNNNNGG 1 cut(s) 18
BsmAI GTCTC 2 cut(s) 699, 1485
BsmFI GGGAC 4 cut(s) 448, 1625, 1862, 1984
BsmI GAATGC 3 cut(s) 864, 1310, 1988
BsnI GGCC 2 cut(s) 119, 1817
BsoBI CYCGRG 1 cut(s) 102
Bsp119I TTCGAA 2 cut(s) 319, 1652
Bsp1286I GDGCHC 4 cut(s) 46, 259, 373, 1368
Bsp1407I TGTACA 2 cut(s) 499, 1337
Bsp143I GATC 9 cut(s) 543, 766, 916, 991, 1048, 1195, 1783, 1878, 2024
BspACI CCGC 9 cut(s) 131, 137, 286, 862, 1052, 1075, 1083, 1283, 1987
BspANI GGCC 2 cut(s) 119, 1817
BspCNI CTCAG 3 cut(s) 322, 583, 900
BspFNI CGCG 2 cut(s) 133, 1989
BspLI GGNNCC 4 cut(s) 449, 617, 1050, 2056
BspMAI CTGCAG 1 cut(s) 1978
BspMI ACCTGC 1 cut(s) 596
BspPI GGATC 3 cut(s) 924, 1043, 1056
BspT104I TTCGAA 2 cut(s) 319, 1652
BspTI CTTAAG 2 cut(s) 584, 1499
BsrDI GCAATG 2 cut(s) 657, 1419
BsrGI TGTACA 2 cut(s) 499, 1337
BsrI ACTGG 5 cut(s) 39, 69, 169, 1913, 1947
BssECI CCNNGG 1 cut(s) 351
BssMI GATC 9 cut(s) 543, 766, 916, 991, 1048, 1195, 1783, 1878, 2024
BssNAI GTATAC 1 cut(s) 417
BssNI GRCGYC 1 cut(s) 1755
BssT1I CCWWGG 1 cut(s) 351
Bst1107I GTATAC 1 cut(s) 417
Bst4CI ACNGT 5 cut(s) 292, 499, 851, 1528, 1740
Bst6I CTCTTC 5 cut(s) 531, 723, 837, 1618, 2017
BstACI GRCGYC 1 cut(s) 1755
BstAFI CTTAAG 2 cut(s) 584, 1499
BstAUI TGTACA 2 cut(s) 499, 1337
BstBAI YACGTR 1 cut(s) 83
BstBI TTCGAA 2 cut(s) 319, 1652
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 4 cut(s) 309, 591, 908, 1427
BstF5I GGATG 8 cut(s) 49, 617, 801, 907, 1279, 1600, 1891, 1960
BstFNI CGCG 2 cut(s) 133, 1989
BstHHI GCGC 1 cut(s) 801
BstKTI GATC 9 cut(s) 546, 769, 919, 994, 1051, 1198, 1786, 1881, 2027
BstMAI GTCTC 2 cut(s) 699, 1485
BstMBI GATC 9 cut(s) 543, 766, 916, 991, 1048, 1195, 1783, 1878, 2024
BstMWI GCNNNNNNNGC 6 cut(s) 565, 1157, 1291, 1604, 1814, 1936
BstNSI RCATGY 1 cut(s) 506
BstPAI GACNNNNGTC 1 cut(s) 132
BstSFI CTRYAG 3 cut(s) 157, 291, 1974
BstSLI GKGCMC 2 cut(s) 46, 1368
BstUI CGCG 2 cut(s) 133, 1989
BstV1I GCAGC 7 cut(s) 543, 693, 1111, 1114, 1135, 1396, 1906
BstV2I GAAGAC 3 cut(s) 1261, 1268, 1439
BstX2I RGATCY 5 cut(s) 543, 916, 991, 1048, 2024
BstYI RGATCY 5 cut(s) 543, 916, 991, 1048, 2024
BstZ17I GTATAC 1 cut(s) 417
Bsu36I CCTNAGG 1 cut(s) 309
BsuI GTATCC 1 cut(s) 1027
BsuRI GGCC 2 cut(s) 119, 1817
BtgZI GCGATG 1 cut(s) 1530
BtsCI GGATG 8 cut(s) 49, 617, 801, 907, 1279, 1600, 1891, 1960
BtsI GCAGTG 2 cut(s) 204, 1292
BtsIMutI CAGTG 6 cut(s) 46, 204, 288, 856, 905, 1292
BveI ACCTGC 1 cut(s) 596
Cac8I GCNNGC 1 cut(s) 339
CfoI GCGC 1 cut(s) 801
Cfr13I GGNCC 5 cut(s) 117, 447, 616, 1910, 2054
CseI GACGC 1 cut(s) 122
Csp6I GTAC 4 cut(s) 500, 847, 1338, 1583
CspCI CAANNNNNGTGG 2 cut(s) 462, 497
CviAII CATG 6 cut(s) 232, 503, 1341, 1438, 1882, 1947
CviQI GTAC 4 cut(s) 500, 847, 1338, 1583
DdeI CTNAG 4 cut(s) 309, 591, 908, 1427
DpnI GATC 9 cut(s) 545, 768, 918, 993, 1050, 1197, 1785, 1880, 2026
DpnII GATC 9 cut(s) 543, 766, 916, 991, 1048, 1195, 1783, 1878, 2024
DriI GACNNNNNGTC 1 cut(s) 1916
Eam1104I CTCTTC 5 cut(s) 531, 723, 837, 1618, 2017
Eam1105I GACNNNNNGTC 1 cut(s) 1916
EarI CTCTTC 5 cut(s) 531, 723, 837, 1618, 2017
Eco130I CCWWGG 1 cut(s) 351
Eco24I GRGCYC 2 cut(s) 259, 373
Eco47I GGWCC 4 cut(s) 447, 616, 1910, 2054
Eco57I CTGAAG 4 cut(s) 555, 1255, 1629, 2018
Eco72I CACGTG 1 cut(s) 83
Eco81I CCTNAGG 1 cut(s) 309
Eco88I CYCGRG 1 cut(s) 102
EcoO109I RGGNCCY 1 cut(s) 447
EcoRI GAATTC 1 cut(s) 186
EcoT14I CCWWGG 1 cut(s) 351
EcoT22I ATGCAT 1 cut(s) 1549
EcoT38I GRGCYC 2 cut(s) 259, 373
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 6 cut(s) 235, 506, 1344, 1441, 1885, 1950
FaqI GGGAC 4 cut(s) 448, 1625, 1862, 1984
FatI CATG 6 cut(s) 231, 502, 1340, 1437, 1881, 1946
FauI CCCGC 1 cut(s) 138
FbaI TGATCA 1 cut(s) 766
FblI GTMKAC 2 cut(s) 294, 416
FokI GGATG 8 cut(s) 36, 624, 808, 914, 1266, 1607, 1898, 1947
FriOI GRGCYC 2 cut(s) 259, 373
FspAI RTGCGCAY 1 cut(s) 800
FspBI CTAG 5 cut(s) 90, 183, 266, 1829, 1835
FspI TGCGCA 1 cut(s) 800
GlaI GCGC 1 cut(s) 800
HaeIII GGCC 2 cut(s) 119, 1817
HgaI GACGC 1 cut(s) 122
HhaI GCGC 1 cut(s) 801
Hin1I GRCGYC 1 cut(s) 1755
Hin1II CATG 6 cut(s) 235, 506, 1344, 1441, 1885, 1950
Hin6I GCGC 1 cut(s) 799
HinP1I GCGC 1 cut(s) 799
HindIII AAGCTT 1 cut(s) 626
HphI GGTGA 1 cut(s) 712
Hpy166II GTNNAC 6 cut(s) 44, 295, 417, 489, 829, 1910
Hpy188I TCNGA 8 cut(s) 223, 408, 466, 1094, 1200, 1300, 1490, 1670
Hpy188III TCNNGA 6 cut(s) 266, 440, 764, 1209, 1474, 1835
Hpy8I GTNNAC 6 cut(s) 44, 295, 417, 489, 829, 1910
Hpy99I CGWCG 1 cut(s) 1757
HpyCH4III ACNGT 5 cut(s) 292, 499, 851, 1528, 1740
HpyCH4IV ACGT 3 cut(s) 82, 835, 1755
HpyF10VI GCNNNNNNNGC 6 cut(s) 565, 1157, 1291, 1604, 1814, 1936
HpyF3I CTNAG 4 cut(s) 309, 591, 908, 1427
HpySE526I ACGT 3 cut(s) 82, 835, 1755
Hsp92I GRCGYC 1 cut(s) 1755
Hsp92II CATG 6 cut(s) 235, 506, 1344, 1441, 1885, 1950
HspAI GCGC 1 cut(s) 799
Ksp22I TGATCA 1 cut(s) 766
Kzo9I GATC 9 cut(s) 543, 766, 916, 991, 1048, 1195, 1783, 1878, 2024
LmnI GCTCC 4 cut(s) 197, 356, 470, 1184
Lsp1109I GCAGC 7 cut(s) 543, 693, 1111, 1114, 1135, 1396, 1906
LweI GCATC 6 cut(s) 786, 810, 892, 1507, 1585, 1876
MaeI CTAG 5 cut(s) 90, 183, 266, 1829, 1835
MaeII ACGT 3 cut(s) 82, 835, 1755
MaeIII GTNAC 5 cut(s) 328, 1012, 1244, 1321, 2087
MalI GATC 9 cut(s) 545, 768, 918, 993, 1050, 1197, 1785, 1880, 2026
MboI GATC 9 cut(s) 543, 766, 916, 991, 1048, 1195, 1783, 1878, 2024
MflI RGATCY 5 cut(s) 543, 916, 991, 1048, 2024
MhlI GDGCHC 4 cut(s) 46, 259, 373, 1368
MlyI GAGTC 5 cut(s) 430, 1009, 1487, 1518, 1851
Mph1103I ATGCAT 1 cut(s) 1549
MroXI GAANNNNTTC 1 cut(s) 732
MseI TTAA 9 cut(s) 585, 807, 1500, 1533, 1676, 1701, 1760, 1866, 1899
MspCI CTTAAG 2 cut(s) 584, 1499
Mva1269I GAATGC 3 cut(s) 864, 1310, 1988
MvnI CGCG 2 cut(s) 133, 1989
MwoI GCNNNNNNNGC 6 cut(s) 565, 1157, 1291, 1604, 1814, 1936
NdeII GATC 9 cut(s) 543, 766, 916, 991, 1048, 1195, 1783, 1878, 2024
NlaIII CATG 6 cut(s) 235, 506, 1344, 1441, 1885, 1950
NlaIV GGNNCC 4 cut(s) 449, 617, 1050, 2056
NmuCI GTSAC 3 cut(s) 1012, 1244, 1321
NsbI TGCGCA 1 cut(s) 800
NsiI ATGCAT 1 cut(s) 1549
NspI RCATGY 1 cut(s) 506
NspV TTCGAA 2 cut(s) 319, 1652
PaqCI CACCTGC 1 cut(s) 596
PciI ACATGT 1 cut(s) 502
PcsI WCGNNNNNNNCGW 1 cut(s) 1063
PctI GAATGC 3 cut(s) 864, 1310, 1988
PdmI GAANNNNTTC 1 cut(s) 732
PfeI GAWTC 5 cut(s) 28, 741, 1095, 1345, 1658
PleI GAGTC 5 cut(s) 430, 1009, 1487, 1517, 1850
PmaCI CACGTG 1 cut(s) 83
PmlI CACGTG 1 cut(s) 83
PpsI GAGTC 5 cut(s) 430, 1009, 1487, 1517, 1850
Ppu21I YACGTR 1 cut(s) 83
PpuMI RGGWCCY 1 cut(s) 447
PscI ACATGT 1 cut(s) 502
PshAI GACNNNNGTC 1 cut(s) 132
Psp5II RGGWCCY 1 cut(s) 447
PspCI CACGTG 1 cut(s) 83
PspN4I GGNNCC 4 cut(s) 449, 617, 1050, 2056
PspPI GGNCC 5 cut(s) 117, 447, 616, 1910, 2054
PspPPI RGGWCCY 1 cut(s) 447
PstI CTGCAG 1 cut(s) 1978
PsuI RGATCY 5 cut(s) 543, 916, 991, 1048, 2024
RsaI GTAC 4 cut(s) 501, 848, 1339, 1584
RsaNI GTAC 4 cut(s) 500, 847, 1338, 1583
SaqAI TTAA 9 cut(s) 585, 807, 1500, 1533, 1676, 1701, 1760, 1866, 1899
Sau3AI GATC 9 cut(s) 543, 766, 916, 991, 1048, 1195, 1783, 1878, 2024
Sau96I GGNCC 5 cut(s) 117, 447, 616, 1910, 2054
SchI GAGTC 5 cut(s) 430, 1009, 1487, 1518, 1851
SduI GDGCHC 4 cut(s) 46, 259, 373, 1368
SfaNI GCATC 6 cut(s) 786, 810, 892, 1507, 1585, 1876
SfcI CTRYAG 3 cut(s) 157, 291, 1974
SfuI TTCGAA 2 cut(s) 319, 1652
SinI GGWCC 4 cut(s) 447, 616, 1910, 2054
SmlI CTYRAG 2 cut(s) 584, 1499
SmoI CTYRAG 2 cut(s) 584, 1499
SsiI CCGC 9 cut(s) 131, 137, 286, 862, 1052, 1075, 1083, 1283, 1987
SspMI CTAG 5 cut(s) 90, 183, 266, 1829, 1835
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 5 cut(s) 292, 499, 851, 1528, 1740
TaiI ACGT 3 cut(s) 85, 838, 1758
TaqI TCGA 6 cut(s) 215, 319, 654, 1066, 1104, 1652
TatI WGTACW 3 cut(s) 499, 846, 1337
TauI GCSGC 3 cut(s) 864, 1085, 1989
TfiI GAWTC 5 cut(s) 28, 741, 1095, 1345, 1658
Tru1I TTAA 9 cut(s) 585, 807, 1500, 1533, 1676, 1701, 1760, 1866, 1899
Tru9I TTAA 9 cut(s) 585, 807, 1500, 1533, 1676, 1701, 1760, 1866, 1899
TscAI CASTG 6 cut(s) 46, 211, 295, 856, 912, 1292
TseFI GTSAC 3 cut(s) 1012, 1244, 1321
TseI GCWGC 7 cut(s) 556, 681, 1124, 1127, 1148, 1409, 1894
Tsp45I GTSAC 3 cut(s) 1012, 1244, 1321
TspDTI ATGAA 5 cut(s) 543, 1087, 1122, 1152, 1268
TspGWI ACGGA 3 cut(s) 608, 949, 1866
TspRI CASTG 6 cut(s) 46, 211, 295, 856, 912, 1292
Vha464I CTTAAG 2 cut(s) 584, 1499
VneI GTGCAC 1 cut(s) 42
VpaK11BI GGWCC 4 cut(s) 447, 616, 1910, 2054
XapI RAATTY 3 cut(s) 186, 686, 1768
XbaI TCTAGA 2 cut(s) 265, 1834
XceI RCATGY 1 cut(s) 506
XmiI GTMKAC 2 cut(s) 294, 416
XmnI GAANNNNTTC 1 cut(s) 732
XspI CTAG 5 cut(s) 90, 183, 266, 1829, 1835
ZraI GACGTC 1 cut(s) 1756
Zsp2I ATGCAT 1 cut(s) 1549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.