Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000394

Physical Location & Seq

Genomic Coordinates Forward (+)

45088514 .. 45091042

Gene Structure 2529 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1323900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 978 bp

ATGAAGGCCGTAGTGATAACGTCTCCAGGTGGGCCTGACGTCCTGAAGCTCCAGGAAGTCGAGGACCCACAGCTCAAGGACGATGAAGTTCTGATCAAAGTAGCCGCCGCGGCGGTGAACGGGTCTGACACGATTCAGAGGAAGGGGGCGTATCCGCCGCCACCGGGTTCCAGCCCCTACCTGGGACTCGAGTGTTCTGGAACCATCGAGGCCGTCGGGAAACACGTGTCGCGCTGGCAAGCCGGTGATCGGGTGTGTGCTCTTCTTAGTGGAGGAGGGTATGCCGAGAAGGTGGCTGTTCCAGCAGGACACGTTTTTCCCATCCCACCTGGTATTTCTATGACGGATGCTGCTAGCTTTCCTGAGGTGGCATGCACTGTTTGGTCGACTGTATTTATGATGAGTCGGCTTTCTGCTGGTGAAACATTTCTGGTTCATGGGGGCTCCAGTGGAATTGGTACATTTGCGATTCAGATAGCCAAATACCAAGGAGCAAGGGTTTTTGTCACAGCGGGAAATGAGGAAAAGTTAGCTGCTTGCAAGAATCTTGGAGCCGATGTATGCATCAATTACAAGACAGAGGACTTTGTTGCACGGGTGAAAGAAGAAACAGGCGGCAAAGGTGTTGATGTTATCTTGGATAGCATTGGGGCAGACTATTTCCGGCGGAACCTTGACAGCTTAAACTTTGATGGGAGGCTTTTTGTTATTGGGCTCATGAGTGGTCCTGTTACAGAACTAGACCTTCGTGCTGTGCTTTCGAGGCGTCTCACCATCCAAGCTGCTGGCTTGCGATACAGAAGTCCCGAGAACAAAGCGGTAATTGTTAGGGAGGTGGAGAACAATGTGTGGCCAGCAATTGTTGCTGGCAAGGTGAAAACTGTGATATACAAGTCTTTTCCGTTATCTGAAGCAGCGGAAGCTCACCGGCTCATGGAAAGTAGCCAGCATATCGGAAAGATACTGCTCGTTGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0003960 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006739 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009117 GO:0009987 GO:0010941 GO:0016491 GO:0016651 GO:0016655 GO:0019362 GO:0019637 GO:0034641 GO:0036094 GO:0042802 GO:0042803 GO:0042981 GO:0043067 GO:0043167 GO:0043168 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046496 GO:0046983 GO:0048037 GO:0048038 GO:0050661 GO:0050662 GO:0050789 GO:0050794 GO:0051186 GO:0055086 GO:0055114 GO:0065007 GO:0070402 GO:0071704 GO:0072524 GO:0097159 GO:1901265 GO:1901360 GO:1901363 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ADH_N ADH_zinc_N

Protein Analysis

326

Amino Acids

34.63

Weight (kDa)

6.54

Isoelectric Point (pI)

46.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ADH_N	PF08240	27 - 109	5.7e-19	Alcohol dehydrogenase GroES-like domain
ADH_zinc_N	PF00107	151 - 266	1.8e-32	Zinc-binding dehydrogenase
ADH_zinc_N_2	PF13602	183 - 323	1.1e-26	Zinc-binding dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000394)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21580
fragaria_vesca	FvH4_3g02190 FvH4_3g02190 FvH4_3g02190 FvH4_3g04320 FvH4_3g04340 FvH4_3g18080 FvH4_3g18080 FvH4_3g18080 FvH4_6g30490 FvH4_6g30490 FvH4_6g30490 FvH4_6g30490
malus_domestica	MD05G1217800.v1.1 MD05G1323900.v1.1 MD12G1074500.v1.1 MD14G1066500.v1.1
prunus_persica	Prupe.4G038700_v2.0.a1 Prupe.7G059000_v2.0.a1 Prupe.7G059000_v2.0.a1 Prupe.7G059300_v2.0.a1 Prupe.7G059300_v2.0.a1 Prupe.7G059300_v2.0.a1 Prupe.7G059300_v2.0.a1
pyrus_communis	pycom05g29820
rosa_chinensis	RchiOBHm_Chr1g0318171 RchiOBHm_Chr5g0003241 RchiOBHm_Chr5g0006701 RchiOBHm_Chr5g0006721 RchiOBHm_Chr5g0006731 RchiOBHm_Chr5g0030211 RchiOBHm_Chr5g0030291 RchiOBHm_Chr5g0030331 RchiOBHm_Chr5g0030371 RchiOBHm_Chr5g0030401 RchiOBHm_Chr5g0030471
rosa_laevigata	RLG00000025923 RLG00000025924 RLG00000030556 RLG00000031088 RLG00000031336 RLG00000031418 RLG00000031420 RLG00000031421 RLG00000031422 RLG00000031423 RLG00000033220
rosa_multiflora	Rmu_sc0001886.1_g000010 Rmu_sc0001886.1_g000011 Rmu_sc0001886.1_g000012 Rmu_sc0010917.1_g000018 Rmu_sc0011424.1_g000013 Rmu_sc0017347.1_g000003 Rmu_sc0017347.1_g000005 Rmu_sc0018387.1_g000006 Rmu_sc0018387.1_g000008
rosa_roxburghii	Rroxscaffold_1G00049810 Rroxscaffold_1G00069320 Rroxscaffold_1G00072770 Rroxscaffold_4G00329990 Rroxscaffold_6G00426520
rosa_rugosa	Rorug01G0019200 Rorug01G0019300 Rorug01G0019400 Rorug02G0611300 Rorug02G0611400 Rorug04G0401400 Rorug04G0423700 Rorug04G0424000 Rorug05G0118000
rosa_samantha	Rh1CG030000 Rh1DG040100 Rh2DG133300 Rh3AG010300 Rh3BG010000 Rh3DG010500 Rh5AG026800 Rh5AG026900 Rh5AG027800 Rh5AG051600 Rh5AG051800 Rh5BG028000 Rh5BG050700 Rh5BG129800 Rh5BG129900 Rh5CG029400 Rh5CG029500 Rh5CG030100 Rh5CG059100 Rh5CG059300 Rh5CG059400 Rh5DG026200 Rh5DG049800 Rh5DG049900 Rh5DG214000
rosa_wichuraiana	Rw1G002510 Rw5G002450 Rw5G002640 Rw5G004650 Rw5G005260 Rw5G005270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	42
AccI	GTMKAC	1 cut(s)	386
AccII	CGCG	2 cut(s)	110, 232
AcoI	YGGCCR	1 cut(s)	851
AcuI	CTGAAG	2 cut(s)	65, 930
AcvI	CACGTG	1 cut(s)	226
AcyI	GRCGYC	2 cut(s)	39, 766
AfaI	GTAC	1 cut(s)	460
AfiI	CCNNNNNNNGG	5 cut(s)	164, 181, 182, 249, 934
AflIII	ACRYGT	3 cut(s)	223, 225, 310
AjnI	CCWGG	4 cut(s)	25, 51, 180, 328
AluBI	AGCT	7 cut(s)	49, 73, 357, 533, 681, 782, 923
AluI	AGCT	7 cut(s)	49, 73, 357, 533, 681, 782, 923
Alw21I	GWGCWC	1 cut(s)	262
Alw26I	GTCTC	2 cut(s)	27, 773
Ama87I	CYCGRG	2 cut(s)	188, 806
AoxI	GGCC	4 cut(s)	6, 32, 210, 851
ApeKI	GCWGC	4 cut(s)	350, 533, 782, 914
Asp700I	GAANNNNTTC	1 cut(s)	426
AspLEI	GCGC	1 cut(s)	234
AspS9I	GGNCC	3 cut(s)	32, 64, 725
AsuC2I	CCSGG	1 cut(s)	165
AsuHPI	GGTGA	7 cut(s)	127, 257, 431, 610, 763, 886, 917
AsuNHI	GCTAGC	1 cut(s)	353
AvaI	CYCGRG	2 cut(s)	188, 806
AvaII	GGWCC	2 cut(s)	64, 725
AxyI	CCTNAGG	1 cut(s)	363
BaeI	ACNNNNGTAYC	2 cut(s)	787, 820
BalI	TGGCCA	1 cut(s)	853
BanII	GRGCYC	2 cut(s)	446, 717
BbrPI	CACGTG	1 cut(s)	226
Bbv12I	GWGCWC	1 cut(s)	262
BbvI	GCAGC	4 cut(s)	337, 520, 769, 926
BccI	CCATC	4 cut(s)	212, 329, 686, 782
BceAI	ACGGC	1 cut(s)	197
BciT130I	CCWGG	4 cut(s)	27, 53, 182, 330
BciVI	GTATCC	1 cut(s)	162
BclI	TGATCA	1 cut(s)	93
BcnI	CCSGG	1 cut(s)	165
BcoDI	GTCTC	2 cut(s)	27, 773
BfaI	CTAG	2 cut(s)	354, 740
BfuI	GTATCC	1 cut(s)	162
BglI	GCCNNNNNGGC	1 cut(s)	110
BisI	GCNGC	9 cut(s)	105, 108, 111, 158, 351, 534, 616, 783, 915
BlsI	GCNGC	9 cut(s)	106, 109, 112, 159, 352, 535, 617, 784, 916
Bme1390I	CCNGG	5 cut(s)	27, 53, 165, 182, 330
Bme18I	GGWCC	2 cut(s)	64, 725
BmeT110I	CYCGRG	2 cut(s)	188, 806
BmgT120I	GGNCC	3 cut(s)	32, 64, 725
BmiI	GGNNCC	6 cut(s)	66, 169, 202, 445, 553, 671
BmrFI	CCNGG	5 cut(s)	27, 53, 165, 182, 330
BmsI	GCATC	2 cut(s)	337, 573
BmtI	GCTAGC	1 cut(s)	357
BpmI	CTGGAG	3 cut(s)	9, 35, 430
BpuEI	CTTGAG	1 cut(s)	59
BpuMI	CCSGG	1 cut(s)	165
BsaAI	YACGTR	1 cut(s)	226
BsaHI	GRCGYC	2 cut(s)	39, 766
BsaJI	CCNNGG	3 cut(s)	108, 181, 487
BsaXI	ACNNNNNCTCC	4 cut(s)	483, 513, 543, 573
Bsc4I	CCNNNNNNNGG	5 cut(s)	164, 181, 182, 249, 934
Bse118I	RCCGGY	2 cut(s)	242, 927
Bse1I	ACTGG	1 cut(s)	447
Bse21I	CCTNAGG	1 cut(s)	363
BseBI	CCWGG	4 cut(s)	27, 53, 182, 330
BseDI	CCNNGG	3 cut(s)	108, 181, 487
BseGI	GGATG	3 cut(s)	321, 352, 774
BseLI	CCNNNNNNNGG	5 cut(s)	164, 181, 182, 249, 934
BseMII	CTCAG	1 cut(s)	354
BseNI	ACTGG	1 cut(s)	447
BseRI	GAGGAG	1 cut(s)	288
BseXI	GCAGC	4 cut(s)	337, 520, 769, 926
Bsh1236I	CGCG	2 cut(s)	110, 232
BshFI	GGCC	4 cut(s)	8, 34, 212, 853
BsiHKAI	GWGCWC	1 cut(s)	262
BsiHKCI	CYCGRG	2 cut(s)	188, 806
BsiSI	CCGG	4 cut(s)	164, 243, 664, 928
BslFI	GGGAC	2 cut(s)	198, 789
BslI	CCNNNNNNNGG	5 cut(s)	164, 181, 182, 249, 934
BsmAI	GTCTC	2 cut(s)	27, 773
BsmBI	CGTCTC	2 cut(s)	27, 773
BsmFI	GGGAC	2 cut(s)	198, 789
BsnI	GGCC	4 cut(s)	8, 34, 212, 853
BsoBI	CYCGRG	2 cut(s)	188, 806
Bsp1286I	GDGCHC	3 cut(s)	262, 446, 717
Bsp143I	GATC	2 cut(s)	93, 247
BspANI	GGCC	4 cut(s)	8, 34, 212, 853
BspCNI	CTCAG	1 cut(s)	355
BspFNI	CGCG	2 cut(s)	110, 232
BspHI	TCATGA	1 cut(s)	717
BspLI	GGNNCC	6 cut(s)	66, 169, 202, 445, 553, 671
BspOI	GCTAGC	1 cut(s)	357
BspQI	GCTCTTC	1 cut(s)	267
BsrFI	RCCGGY	2 cut(s)	242, 927
BsrI	ACTGG	1 cut(s)	447
BssAI	RCCGGY	2 cut(s)	242, 927
BssECI	CCNNGG	3 cut(s)	108, 181, 487
BssMI	GATC	2 cut(s)	93, 247
BssNI	GRCGYC	2 cut(s)	39, 766
BssT1I	CCWWGG	1 cut(s)	487
Bst2UI	CCWGG	4 cut(s)	27, 53, 182, 330
Bst4CI	ACNGT	3 cut(s)	379, 391, 883
Bst6I	CTCTTC	1 cut(s)	267
BstACI	GRCGYC	2 cut(s)	39, 766
BstAPI	GCANNNNNTGC	1 cut(s)	863
BstBAI	YACGTR	1 cut(s)	226
BstDEI	CTNAG	2 cut(s)	266, 363
BstDSI	CCRYGG	1 cut(s)	108
BstF5I	GGATG	3 cut(s)	321, 352, 774
BstFNI	CGCG	2 cut(s)	110, 232
BstHHI	GCGC	1 cut(s)	234
BstKTI	GATC	2 cut(s)	96, 250
BstMAI	GTCTC	2 cut(s)	27, 773
BstMBI	GATC	2 cut(s)	93, 247
BstMWI	GCNNNNNNNGC	5 cut(s)	110, 302, 763, 863, 920
BstNI	CCWGG	4 cut(s)	27, 53, 182, 330
BstNSI	RCATGY	1 cut(s)	375
BstSCI	CCNGG	5 cut(s)	25, 51, 163, 180, 328
BstUI	CGCG	2 cut(s)	110, 232
BstV1I	GCAGC	4 cut(s)	337, 520, 769, 926
BstXI	CCANNNNNNTGG	1 cut(s)	785
Bsu36I	CCTNAGG	1 cut(s)	363
BsuI	GTATCC	1 cut(s)	162
BsuRI	GGCC	4 cut(s)	8, 34, 212, 853
BtgI	CCRYGG	1 cut(s)	108
BtsCI	GGATG	3 cut(s)	321, 352, 774
BtsIMutI	CAGTG	2 cut(s)	375, 454
CciI	TCATGA	1 cut(s)	717
CfoI	GCGC	1 cut(s)	234
Cfr10I	RCCGGY	2 cut(s)	242, 927
Cfr13I	GGNCC	3 cut(s)	32, 64, 725
Cfr42I	CCGCGG	1 cut(s)	111
CseI	GACGC	1 cut(s)	755
CsiI	ACCWGGT	1 cut(s)	328
Csp6I	GTAC	1 cut(s)	459
CviAII	CATG	4 cut(s)	372, 437, 718, 934
CviQI	GTAC	1 cut(s)	459
DdeI	CTNAG	2 cut(s)	266, 363
DpnI	GATC	2 cut(s)	95, 249
DpnII	GATC	2 cut(s)	93, 247
EaeI	YGGCCR	1 cut(s)	851
Eam1104I	CTCTTC	1 cut(s)	267
EarI	CTCTTC	1 cut(s)	267
EciI	GGCGGA	2 cut(s)	144, 682
Eco130I	CCWWGG	1 cut(s)	487
Eco24I	GRGCYC	2 cut(s)	446, 717
Eco47I	GGWCC	2 cut(s)	64, 725
Eco57I	CTGAAG	2 cut(s)	65, 930
Eco72I	CACGTG	1 cut(s)	226
Eco81I	CCTNAGG	1 cut(s)	363
Eco88I	CYCGRG	2 cut(s)	188, 806
EcoO109I	RGGNCCY	1 cut(s)	64
EcoRII	CCWGG	4 cut(s)	25, 51, 180, 328
EcoT14I	CCWWGG	1 cut(s)	487
EcoT22I	ATGCAT	1 cut(s)	566
EcoT38I	GRGCYC	2 cut(s)	446, 717
ErhI	CCWWGG	1 cut(s)	487
Esp3I	CGTCTC	2 cut(s)	27, 773
FaeI	CATG	4 cut(s)	375, 440, 721, 937
FaqI	GGGAC	2 cut(s)	198, 789
FatI	CATG	4 cut(s)	371, 436, 717, 933
FauI	CCCGC	1 cut(s)	505
FbaI	TGATCA	1 cut(s)	93
FblI	GTMKAC	1 cut(s)	386
Fnu4HI	GCNGC	9 cut(s)	105, 108, 111, 158, 351, 534, 616, 783, 915
FokI	GGATG	3 cut(s)	308, 359, 761
FriOI	GRGCYC	2 cut(s)	446, 717
Fsp4HI	GCNGC	9 cut(s)	105, 108, 111, 158, 351, 534, 616, 783, 915
FspBI	CTAG	2 cut(s)	354, 740
GlaI	GCGC	1 cut(s)	233
GluI	GCNGC	9 cut(s)	105, 108, 111, 158, 351, 534, 616, 783, 915
GsuI	CTGGAG	3 cut(s)	9, 35, 430
HaeIII	GGCC	4 cut(s)	8, 34, 212, 853
HapII	CCGG	4 cut(s)	164, 243, 664, 928
HgaI	GACGC	1 cut(s)	755
HhaI	GCGC	1 cut(s)	234
Hin1I	GRCGYC	2 cut(s)	39, 766
Hin1II	CATG	4 cut(s)	375, 440, 721, 937
Hin6I	GCGC	1 cut(s)	232
HinP1I	GCGC	1 cut(s)	232
HincII	GTYRAC	1 cut(s)	387
HindII	GTYRAC	1 cut(s)	387
HinfI	GANTC	5 cut(s)	133, 186, 403, 469, 544
HpaII	CCGG	4 cut(s)	164, 243, 664, 928
HphI	GGTGA	7 cut(s)	127, 257, 431, 610, 763, 886, 917
Hpy166II	GTNNAC	2 cut(s)	118, 387
Hpy188I	TCNGA	6 cut(s)	93, 127, 138, 474, 910, 956
Hpy188III	TCNNGA	6 cut(s)	43, 198, 217, 362, 718, 806
Hpy8I	GTNNAC	2 cut(s)	118, 387
Hpy99I	CGWCG	1 cut(s)	218
HpyAV	CCTTC	3 cut(s)	136, 283, 755
HpyCH4III	ACNGT	3 cut(s)	379, 391, 883
HpyCH4IV	ACGT	4 cut(s)	20, 39, 225, 312
HpyCH4V	TGCA	4 cut(s)	375, 540, 564, 593
HpyF10VI	GCNNNNNNNGC	5 cut(s)	110, 302, 763, 863, 920
HpyF3I	CTNAG	2 cut(s)	266, 363
HpySE526I	ACGT	4 cut(s)	20, 39, 225, 312
Hsp92I	GRCGYC	2 cut(s)	39, 766
Hsp92II	CATG	4 cut(s)	375, 440, 721, 937
HspAI	GCGC	1 cut(s)	232
Ksp22I	TGATCA	1 cut(s)	93
KspI	CCGCGG	1 cut(s)	111
Kzo9I	GATC	2 cut(s)	93, 247
LguI	GCTCTTC	1 cut(s)	267
LmnI	GCTCC	4 cut(s)	54, 449, 491, 551
Lsp1109I	GCAGC	4 cut(s)	337, 520, 769, 926
LweI	GCATC	2 cut(s)	337, 573
MabI	ACCWGGT	1 cut(s)	328
MaeI	CTAG	2 cut(s)	354, 740
MaeII	ACGT	4 cut(s)	20, 39, 225, 312
MaeIII	GTNAC	2 cut(s)	505, 730
MalI	GATC	2 cut(s)	95, 249
MboI	GATC	2 cut(s)	93, 247
MboII	GAAGA	2 cut(s)	254, 617
MfeI	CAATTG	1 cut(s)	858
MhlI	GDGCHC	3 cut(s)	262, 446, 717
MlsI	TGGCCA	1 cut(s)	853
MluCI	AATT	4 cut(s)	453, 568, 822, 858
MluNI	TGGCCA	1 cut(s)	853
MlyI	GAGTC	2 cut(s)	180, 412
Mox20I	TGGCCA	1 cut(s)	853
Mph1103I	ATGCAT	1 cut(s)	566
MroXI	GAANNNNTTC	1 cut(s)	426
MscI	TGGCCA	1 cut(s)	853
MseI	TTAA	1 cut(s)	683
Msp20I	TGGCCA	1 cut(s)	853
MspA1I	CMGCKG	3 cut(s)	110, 512, 917
MspI	CCGG	4 cut(s)	164, 243, 664, 928
MspR9I	CCNGG	5 cut(s)	27, 53, 165, 182, 330
MunI	CAATTG	1 cut(s)	858
MvaI	CCWGG	4 cut(s)	27, 53, 182, 330
MvnI	CGCG	2 cut(s)	110, 232
MwoI	GCNNNNNNNGC	5 cut(s)	110, 302, 763, 863, 920
NciI	CCSGG	1 cut(s)	165
NdeII	GATC	2 cut(s)	93, 247
NheI	GCTAGC	1 cut(s)	353
NlaIII	CATG	4 cut(s)	375, 440, 721, 937
NlaIV	GGNNCC	6 cut(s)	66, 169, 202, 445, 553, 671
NmeAIII	GCCGAG	1 cut(s)	310
NmuCI	GTSAC	1 cut(s)	505
NsiI	ATGCAT	1 cut(s)	566
NspI	RCATGY	1 cut(s)	375
PaeI	GCATGC	1 cut(s)	375
PaeR7I	CTCGAG	1 cut(s)	188
PagI	TCATGA	1 cut(s)	717
PciSI	GCTCTTC	1 cut(s)	267
PcsI	WCGNNNNNNNCGW	1 cut(s)	222
PdmI	GAANNNNTTC	1 cut(s)	426
PfeI	GAWTC	3 cut(s)	133, 469, 544
PfoI	TCCNGGA	1 cut(s)	51
PkrI	GCNGC	9 cut(s)	106, 109, 112, 159, 352, 535, 617, 784, 916
PleI	GAGTC	2 cut(s)	180, 411
PmaCI	CACGTG	1 cut(s)	226
PmlI	CACGTG	1 cut(s)	226
PpsI	GAGTC	2 cut(s)	180, 411
Ppu21I	YACGTR	1 cut(s)	226
PpuMI	RGGWCCY	1 cut(s)	64
Psp5II	RGGWCCY	1 cut(s)	64
Psp6I	CCWGG	4 cut(s)	25, 51, 180, 328
PspCI	CACGTG	1 cut(s)	226
PspGI	CCWGG	4 cut(s)	25, 51, 180, 328
PspN4I	GGNNCC	6 cut(s)	66, 169, 202, 445, 553, 671
PspPI	GGNCC	3 cut(s)	32, 64, 725
PspPPI	RGGWCCY	1 cut(s)	64
PspXI	VCTCGAGB	1 cut(s)	188
RsaI	GTAC	1 cut(s)	460
RsaNI	GTAC	1 cut(s)	459
SacII	CCGCGG	1 cut(s)	111
SalI	GTCGAC	1 cut(s)	385
SapI	GCTCTTC	1 cut(s)	267
SaqAI	TTAA	1 cut(s)	683
SatI	GCNGC	9 cut(s)	105, 108, 111, 158, 351, 534, 616, 783, 915
Sau3AI	GATC	2 cut(s)	93, 247
Sau96I	GGNCC	3 cut(s)	32, 64, 725
SchI	GAGTC	2 cut(s)	180, 412
ScrFI	CCNGG	5 cut(s)	27, 53, 165, 182, 330
SduI	GDGCHC	3 cut(s)	262, 446, 717
SexAI	ACCWGGT	1 cut(s)	328
SfaNI	GCATC	2 cut(s)	337, 573
Sfr274I	CTCGAG	1 cut(s)	188
Sfr303I	CCGCGG	1 cut(s)	111
SgrBI	CCGCGG	1 cut(s)	111
SinI	GGWCC	2 cut(s)	64, 725
SlaI	CTCGAG	1 cut(s)	188
SmlI	CTYRAG	2 cut(s)	74, 188
SmoI	CTYRAG	2 cut(s)	74, 188
SphI	GCATGC	1 cut(s)	375
Sse9I	AATT	4 cut(s)	453, 568, 822, 858
SspMI	CTAG	2 cut(s)	354, 740
StyD4I	CCNGG	5 cut(s)	25, 51, 163, 180, 328
StyI	CCWWGG	1 cut(s)	487
TaaI	ACNGT	3 cut(s)	379, 391, 883
TaiI	ACGT	4 cut(s)	23, 42, 228, 315
TaqI	TCGA	5 cut(s)	60, 189, 207, 386, 761
TasI	AATT	4 cut(s)	453, 568, 822, 858
TauI	GCSGC	5 cut(s)	107, 110, 113, 160, 618
TfiI	GAWTC	3 cut(s)	133, 469, 544
Tru1I	TTAA	1 cut(s)	683
Tru9I	TTAA	1 cut(s)	683
TscAI	CASTG	2 cut(s)	382, 454
TseFI	GTSAC	1 cut(s)	505
TseI	GCWGC	4 cut(s)	350, 533, 782, 914
Tsp45I	GTSAC	1 cut(s)	505
TspDTI	ATGAA	3 cut(s)	17, 99, 425
TspGWI	ACGGA	2 cut(s)	359, 891
TspRI	CASTG	2 cut(s)	382, 454
VpaK11BI	GGWCC	2 cut(s)	64, 725
XceI	RCATGY	1 cut(s)	375
XcmI	CCANNNNNNNNNTGG	1 cut(s)	178
XhoI	CTCGAG	1 cut(s)	188
XmiI	GTMKAC	1 cut(s)	386
XmnI	GAANNNNTTC	1 cut(s)	426
XspI	CTAG	2 cut(s)	354, 740
ZraI	GACGTC	1 cut(s)	40
Zsp2I	ATGCAT	1 cut(s)	566

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.