FvH4_3g02190

quinone oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000394
Physical Location & Seq
Forward (+)
1113776 .. 1119852
6077 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g02190.t1

Sequence Viewer

Length: 1164 bp
ATGAAAATGCACAAGAATCTGCAACTCGGAGGTGGATCCAAGAGAGGAAAAGAGAGTAAGCGCAATGAGAGAGTTCATAGTAAGACTGAGTCGCCGACGTCGTCTGGCCCAAAGAAGGTGATGCAGGCGGTTGTTGTTAGGGGTGAAGGTGGCGGACCTGAGACCCTCCAGGTACAAACGGTGCCAAGACCAATTCCCAAACAAGATGAGGTACTCGTCAAGGTGGACTACGCCGGAGTCAACAGGCCGGACATTCACCAGAGGGAGGACATGTTACCACCAGATGATAGAAGGAGCCACTACCTCGGACTTGAATGTTCGGGATCCATCACTGAGTTTGGAGTAGGCATGTCCAACGAAGACAAGAAAGCCTGGAAACTAGGCGACAAGGTGTGTGCTATTACCAATGGAGGTAGCTATGCGGAATACGTGGCTGTTCCCGTTGGACATTTGCTTCCTATTCCTTCTAGTACAAATCTACAGGACGCTGCCACATTTCCTCAAGCTGCTTGTACGGTGTCGTCGGAGCTTTTTATGAAGGAAAGGTTATCTCTTCGTATGGTGTCTGAGGAACCGGGTTCCCGGCTATCGTCCGGTGAAAATCAAACACTTTTGATTCATGGGGGTTCAAGTGGTATTGGAACATTTGCTATTCAAATGGCTAAACATCAGGGGGTGACTGTATATGTCACTGCAGGTAGTGATGAGAAACTTAAAGTTTGTGAGAAGCTTGGAGCCGATTTTTGCATCAATTATCATAAGGAAGAGTTTGAAAAGGAGGTACTGAAGAAAACAGCAGGCAAAGGTGTAAATGTCATCTTGGATTGCCTTGGAGATAAGTATTTTCTGCGAAACATATCTTGCTTAAGTTCCGGGGGTTGGCTCGTCACAATTGGTCCTACCTGTAACACATCAGTACAAGATTCTTACACCTTTCCTTCAGAGCGTCACCAGGTTGAAGCGTGTTCCCTTCGAAACAGAACTTCAGAGGATAAAGCTGAGATTGTGGAGGAGGTGAAGAAGCATGTCTGGCCTGCAATTGAGGCTGGTAAGGTGAAAGCTTATGAAAACAATATCAAGCCTTATCCCTTTAAGCATGCAGCAGACGCTCACAAACGCATGGAGAATGGCAATTTCATCGGGAAGATTTTGCTTTCTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

388

Amino Acids

42.39

Weight (kDa)

8.43

Isoelectric Point (pI)

55.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 68 - 155 1.8e-12 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 212 - 326 1.8e-25 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 244 - 385 1.9e-18 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000394)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21580
fragaria_vesca FvH4_3g02190 FvH4_3g02190 FvH4_3g02190 FvH4_3g04320 FvH4_3g04340 FvH4_3g18080 FvH4_3g18080 FvH4_3g18080 FvH4_6g30490 FvH4_6g30490 FvH4_6g30490 FvH4_6g30490
malus_domestica MD05G1217800.v1.1 MD05G1323900.v1.1 MD12G1074500.v1.1 MD14G1066500.v1.1
prunus_persica Prupe.4G038700_v2.0.a1 Prupe.7G059000_v2.0.a1 Prupe.7G059000_v2.0.a1 Prupe.7G059300_v2.0.a1 Prupe.7G059300_v2.0.a1 Prupe.7G059300_v2.0.a1 Prupe.7G059300_v2.0.a1
pyrus_communis pycom05g29820
rosa_chinensis RchiOBHm_Chr1g0318171 RchiOBHm_Chr5g0003241 RchiOBHm_Chr5g0006701 RchiOBHm_Chr5g0006721 RchiOBHm_Chr5g0006731 RchiOBHm_Chr5g0030211 RchiOBHm_Chr5g0030291 RchiOBHm_Chr5g0030331 RchiOBHm_Chr5g0030371 RchiOBHm_Chr5g0030401 RchiOBHm_Chr5g0030471
rosa_laevigata RLG00000025923 RLG00000025924 RLG00000030556 RLG00000031088 RLG00000031336 RLG00000031418 RLG00000031420 RLG00000031421 RLG00000031422 RLG00000031423 RLG00000033220
rosa_multiflora Rmu_sc0001886.1_g000010 Rmu_sc0001886.1_g000011 Rmu_sc0001886.1_g000012 Rmu_sc0010917.1_g000018 Rmu_sc0011424.1_g000013 Rmu_sc0017347.1_g000003 Rmu_sc0017347.1_g000005 Rmu_sc0018387.1_g000006 Rmu_sc0018387.1_g000008
rosa_roxburghii Rroxscaffold_1G00049810 Rroxscaffold_1G00069320 Rroxscaffold_1G00072770 Rroxscaffold_4G00329990 Rroxscaffold_6G00426520
rosa_rugosa Rorug01G0019200 Rorug01G0019300 Rorug01G0019400 Rorug02G0611300 Rorug02G0611400 Rorug04G0401400 Rorug04G0423700 Rorug04G0424000 Rorug05G0118000
rosa_samantha Rh1CG030000 Rh1DG040100 Rh2DG133300 Rh3AG010300 Rh3BG010000 Rh3DG010500 Rh5AG026800 Rh5AG026900 Rh5AG027800 Rh5AG051600 Rh5AG051800 Rh5BG028000 Rh5BG050700 Rh5BG129800 Rh5BG129900 Rh5CG029400 Rh5CG029500 Rh5CG030100 Rh5CG059100 Rh5CG059300 Rh5CG059400 Rh5DG026200 Rh5DG049800 Rh5DG049900 Rh5DG214000
rosa_wichuraiana Rw1G002510 Rw5G002450 Rw5G002640 Rw5G004650 Rw5G005260 Rw5G005270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 101
Acc36I ACCTGC 1 cut(s) 686
AccB1I GGYRCC 1 cut(s) 181
AciI CCGC 3 cut(s) 128, 153, 422
AclWI GGATC 4 cut(s) 30, 43, 318, 331
AcuI CTGAAG 3 cut(s) 806, 924, 969
AcyI GRCGYC 1 cut(s) 98
AfaI GTAC 6 cut(s) 174, 213, 472, 514, 783, 918
AfiI CCNNNNNNNGG 3 cut(s) 115, 265, 879
AflII CTTAAG 1 cut(s) 865
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 5 cut(s) 314, 630, 656, 773, 959
AjnI CCWGG 3 cut(s) 168, 371, 951
AluBI AGCT 6 cut(s) 417, 506, 529, 730, 998, 1061
AluI AGCT 6 cut(s) 417, 506, 529, 730, 998, 1061
Alw26I GTCTC 1 cut(s) 155
AlwI GGATC 4 cut(s) 30, 43, 318, 331
AoxI GGCC 3 cut(s) 106, 245, 1031
ApeKI GCWGC 3 cut(s) 488, 506, 1100
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 3 cut(s) 107, 155, 896
AsuC2I CCSGG 3 cut(s) 576, 583, 874
AsuHPI GGTGA 8 cut(s) 130, 155, 248, 608, 688, 941, 1027, 1066
AsuII TTCGAA 1 cut(s) 973
AvaII GGWCC 2 cut(s) 155, 896
BamHI GGATCC 2 cut(s) 35, 323
BanI GGYRCC 1 cut(s) 181
BbsI GAAGAC 1 cut(s) 366
BbvI GCAGC 3 cut(s) 475, 493, 1112
BccI CCATC 1 cut(s) 335
BcgI CGANNNNNNTGC 2 cut(s) 1120, 1154
BciT130I CCWGG 3 cut(s) 170, 373, 953
BcnI CCSGG 3 cut(s) 576, 583, 874
BcoDI GTCTC 1 cut(s) 155
BfaI CTAG 2 cut(s) 380, 468
BfmI CTRYAG 2 cut(s) 479, 693
BfrI CTTAAG 1 cut(s) 865
BfuAI ACCTGC 1 cut(s) 686
BisI GCNGC 3 cut(s) 489, 507, 1101
BlsI GCNGC 3 cut(s) 490, 508, 1102
Bme1390I CCNGG 6 cut(s) 170, 373, 576, 583, 874, 953
Bme18I GGWCC 2 cut(s) 155, 896
BmgT120I GGNCC 3 cut(s) 107, 155, 896
BmiI GGNNCC 7 cut(s) 37, 183, 296, 325, 573, 580, 736
BmrFI CCNGG 6 cut(s) 170, 373, 576, 583, 874, 953
BmsI GCATC 2 cut(s) 111, 756
BpiI GAAGAC 1 cut(s) 366
BpmI CTGGAG 1 cut(s) 152
Bpu14I TTCGAA 1 cut(s) 973
BpuEI CTTGAG 1 cut(s) 486
BpuMI CCSGG 3 cut(s) 576, 583, 874
BsaAI YACGTR 1 cut(s) 430
BsaHI GRCGYC 1 cut(s) 98
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 3 cut(s) 304, 829, 873
BsaWI WCCGGW 1 cut(s) 593
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 3 cut(s) 115, 265, 879
Bse3DI GCAATG 1 cut(s) 70
BseBI CCWGG 3 cut(s) 170, 373, 953
BseDI CCNNGG 3 cut(s) 304, 829, 873
BseLI CCNNNNNNNGG 3 cut(s) 115, 265, 879
BseMI GCAATG 1 cut(s) 70
BseMII CTCAG 5 cut(s) 78, 150, 324, 558, 990
BseRI GAGGAG 1 cut(s) 1025
BseXI GCAGC 3 cut(s) 475, 493, 1112
BshFI GGCC 3 cut(s) 108, 247, 1033
BshNI GGYRCC 1 cut(s) 181
BsiSI CCGG 6 cut(s) 234, 248, 575, 583, 594, 873
BslI CCNNNNNNNGG 3 cut(s) 115, 265, 879
BsmAI GTCTC 1 cut(s) 155
BsnI GGCC 3 cut(s) 108, 247, 1033
Bso31I GGTCTC 1 cut(s) 155
Bsp119I TTCGAA 1 cut(s) 973
Bsp143I GATC 2 cut(s) 35, 323
BspACI CCGC 3 cut(s) 128, 153, 422
BspANI GGCC 3 cut(s) 108, 247, 1033
BspCNI CTCAG 5 cut(s) 79, 151, 325, 559, 991
BspLI GGNNCC 7 cut(s) 37, 183, 296, 325, 573, 580, 736
BspMAI CTGCAG 1 cut(s) 697
BspMI ACCTGC 1 cut(s) 686
BspPI GGATC 4 cut(s) 30, 43, 318, 331
BspT104I TTCGAA 1 cut(s) 973
BspT107I GGYRCC 1 cut(s) 181
BspTI CTTAAG 1 cut(s) 865
BspTNI GGTCTC 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 70
BssECI CCNNGG 3 cut(s) 304, 829, 873
BssMI GATC 2 cut(s) 35, 323
BssNI GRCGYC 1 cut(s) 98
BssT1I CCWWGG 1 cut(s) 829
Bst2UI CCWGG 3 cut(s) 170, 373, 953
Bst4CI ACNGT 3 cut(s) 181, 517, 682
Bst6I CTCTTC 2 cut(s) 558, 759
BstACI GRCGYC 1 cut(s) 98
BstAFI CTTAAG 1 cut(s) 865
BstBAI YACGTR 1 cut(s) 430
BstBI TTCGAA 1 cut(s) 973
BstC8I GCNNGC 4 cut(s) 126, 799, 1035, 1098
BstDEI CTNAG 5 cut(s) 87, 159, 333, 567, 999
BstHHI GCGC 1 cut(s) 63
BstKTI GATC 2 cut(s) 38, 326
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 2 cut(s) 35, 323
BstMWI GCNNNNNNNGC 3 cut(s) 1030, 1043, 1106
BstNI CCWGG 3 cut(s) 170, 373, 953
BstNSI RCATGY 4 cut(s) 274, 352, 1028, 1100
BstSCI CCNGG 6 cut(s) 168, 371, 574, 581, 872, 951
BstSFI CTRYAG 2 cut(s) 479, 693
BstV1I GCAGC 3 cut(s) 475, 493, 1112
BstV2I GAAGAC 1 cut(s) 366
BstX2I RGATCY 2 cut(s) 35, 323
BstYI RGATCY 2 cut(s) 35, 323
BsuRI GGCC 3 cut(s) 108, 247, 1033
BtsI GCAGTG 1 cut(s) 690
BtsIMutI CAGTG 2 cut(s) 330, 690
BveI ACCTGC 1 cut(s) 686
Cac8I GCNNGC 4 cut(s) 126, 799, 1035, 1098
CfoI GCGC 1 cut(s) 63
Cfr13I GGNCC 3 cut(s) 107, 155, 896
CseI GACGC 3 cut(s) 494, 935, 1115
CsiI ACCWGGT 1 cut(s) 951
Csp6I GTAC 6 cut(s) 173, 212, 471, 513, 782, 917
CviAII CATG 6 cut(s) 271, 349, 620, 1025, 1097, 1120
CviQI GTAC 6 cut(s) 173, 212, 471, 513, 782, 917
DdeI CTNAG 5 cut(s) 87, 159, 333, 567, 999
DpnI GATC 2 cut(s) 37, 325
DpnII GATC 2 cut(s) 35, 323
Eam1104I CTCTTC 2 cut(s) 558, 759
EarI CTCTTC 2 cut(s) 558, 759
EciI GGCGGA 1 cut(s) 168
Eco130I CCWWGG 1 cut(s) 829
Eco31I GGTCTC 1 cut(s) 155
Eco47I GGWCC 2 cut(s) 155, 896
Eco57I CTGAAG 3 cut(s) 806, 924, 969
EcoRII CCWGG 3 cut(s) 168, 371, 951
EcoT14I CCWWGG 1 cut(s) 829
ErhI CCWWGG 1 cut(s) 829
FaeI CATG 6 cut(s) 274, 352, 623, 1028, 1100, 1123
FatI CATG 6 cut(s) 270, 348, 619, 1024, 1096, 1119
Fnu4HI GCNGC 3 cut(s) 489, 507, 1101
Fsp4HI GCNGC 3 cut(s) 489, 507, 1101
FspBI CTAG 2 cut(s) 380, 468
GlaI GCGC 1 cut(s) 62
GluI GCNGC 3 cut(s) 489, 507, 1101
GsuI CTGGAG 1 cut(s) 152
HaeIII GGCC 3 cut(s) 108, 247, 1033
HapII CCGG 6 cut(s) 234, 248, 575, 583, 594, 873
HgaI GACGC 3 cut(s) 494, 935, 1115
HhaI GCGC 1 cut(s) 63
Hin1I GRCGYC 1 cut(s) 98
Hin1II CATG 6 cut(s) 274, 352, 623, 1028, 1100, 1123
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HindIII AAGCTT 2 cut(s) 728, 1059
HinfI GANTC 5 cut(s) 16, 89, 237, 616, 923
HpaII CCGG 6 cut(s) 234, 248, 575, 583, 594, 873
HphI GGTGA 8 cut(s) 130, 155, 248, 608, 688, 941, 1027, 1066
Hpy166II GTNNAC 2 cut(s) 226, 241
Hpy188I TCNGA 6 cut(s) 29, 308, 526, 568, 943, 988
Hpy188III TCNNGA 2 cut(s) 321, 1141
Hpy8I GTNNAC 2 cut(s) 226, 241
Hpy99I CGWCG 3 cut(s) 100, 103, 526
HpyAV CCTTC 7 cut(s) 109, 140, 285, 474, 532, 948, 980
HpyCH4III ACNGT 3 cut(s) 181, 517, 682
HpyCH4IV ACGT 2 cut(s) 98, 429
HpyCH4V TGCA 7 cut(s) 10, 22, 124, 695, 747, 1037, 1100
HpyF10VI GCNNNNNNNGC 3 cut(s) 1030, 1043, 1106
HpyF3I CTNAG 5 cut(s) 87, 159, 333, 567, 999
HpySE526I ACGT 2 cut(s) 98, 429
Hsp92I GRCGYC 1 cut(s) 98
Hsp92II CATG 6 cut(s) 274, 352, 623, 1028, 1100, 1123
HspAI GCGC 1 cut(s) 61
Kzo9I GATC 2 cut(s) 35, 323
LmnI GCTCC 3 cut(s) 294, 526, 734
Lsp1109I GCAGC 3 cut(s) 475, 493, 1112
LweI GCATC 2 cut(s) 111, 756
MabI ACCWGGT 1 cut(s) 951
MaeI CTAG 2 cut(s) 380, 468
MaeII ACGT 2 cut(s) 98, 429
MaeIII GTNAC 6 cut(s) 273, 676, 688, 886, 905, 947
MalI GATC 2 cut(s) 37, 325
MboI GATC 2 cut(s) 35, 323
MboII GAAGA 6 cut(s) 371, 545, 776, 799, 1030, 1156
MfeI CAATTG 2 cut(s) 891, 1038
MflI RGATCY 2 cut(s) 35, 323
MluCI AATT 5 cut(s) 192, 751, 891, 1038, 1132
MlyI GAGTC 2 cut(s) 98, 246
MmeI TCCRAC 3 cut(s) 378, 424, 504
MseI TTAA 3 cut(s) 714, 866, 1092
MspCI CTTAAG 1 cut(s) 865
MspI CCGG 6 cut(s) 234, 248, 575, 583, 594, 873
MspR9I CCNGG 6 cut(s) 170, 373, 576, 583, 874, 953
MunI CAATTG 2 cut(s) 891, 1038
MvaI CCWGG 3 cut(s) 170, 373, 953
MwoI GCNNNNNNNGC 3 cut(s) 1030, 1043, 1106
NciI CCSGG 3 cut(s) 576, 583, 874
NdeII GATC 2 cut(s) 35, 323
NlaIII CATG 6 cut(s) 274, 352, 623, 1028, 1100, 1123
NlaIV GGNNCC 7 cut(s) 37, 183, 296, 325, 573, 580, 736
NmuCI GTSAC 4 cut(s) 676, 688, 886, 947
NspI RCATGY 4 cut(s) 274, 352, 1028, 1100
NspV TTCGAA 1 cut(s) 973
PaeI GCATGC 1 cut(s) 1100
PciI ACATGT 1 cut(s) 270
PcsI WCGNNNNNNNCGW 1 cut(s) 98
PfeI GAWTC 3 cut(s) 16, 616, 923
PflFI GACNNNGTC 2 cut(s) 88, 100
PkrI GCNGC 3 cut(s) 490, 508, 1102
PleI GAGTC 2 cut(s) 97, 245
PpsI GAGTC 2 cut(s) 97, 245
Ppu21I YACGTR 1 cut(s) 430
PscI ACATGT 1 cut(s) 270
Psp6I CCWGG 3 cut(s) 168, 371, 951
PspGI CCWGG 3 cut(s) 168, 371, 951
PspN4I GGNNCC 7 cut(s) 37, 183, 296, 325, 573, 580, 736
PspPI GGNCC 3 cut(s) 107, 155, 896
PstI CTGCAG 1 cut(s) 697
PsuI RGATCY 2 cut(s) 35, 323
PsyI GACNNNGTC 2 cut(s) 88, 100
RsaI GTAC 6 cut(s) 174, 213, 472, 514, 783, 918
RsaNI GTAC 6 cut(s) 173, 212, 471, 513, 782, 917
SaqAI TTAA 3 cut(s) 714, 866, 1092
SatI GCNGC 3 cut(s) 489, 507, 1101
Sau3AI GATC 2 cut(s) 35, 323
Sau96I GGNCC 3 cut(s) 107, 155, 896
SchI GAGTC 2 cut(s) 98, 246
ScrFI CCNGG 6 cut(s) 170, 373, 576, 583, 874, 953
SexAI ACCWGGT 1 cut(s) 951
SfaNI GCATC 2 cut(s) 111, 756
SfcI CTRYAG 2 cut(s) 479, 693
SfuI TTCGAA 1 cut(s) 973
SinI GGWCC 2 cut(s) 155, 896
SmlI CTYRAG 2 cut(s) 501, 865
SmoI CTYRAG 2 cut(s) 501, 865
SphI GCATGC 1 cut(s) 1100
Sse9I AATT 5 cut(s) 192, 751, 891, 1038, 1132
SsiI CCGC 3 cut(s) 128, 153, 422
SspMI CTAG 2 cut(s) 380, 468
StyD4I CCNGG 6 cut(s) 168, 371, 574, 581, 872, 951
StyI CCWWGG 1 cut(s) 829
TaaI ACNGT 3 cut(s) 181, 517, 682
TaiI ACGT 2 cut(s) 101, 432
TaqI TCGA 1 cut(s) 973
TasI AATT 5 cut(s) 192, 751, 891, 1038, 1132
TatI WGTACW 2 cut(s) 470, 916
TfiI GAWTC 3 cut(s) 16, 616, 923
Tru1I TTAA 3 cut(s) 714, 866, 1092
Tru9I TTAA 3 cut(s) 714, 866, 1092
TscAI CASTG 2 cut(s) 337, 697
TseFI GTSAC 4 cut(s) 676, 688, 886, 947
TseI GCWGC 3 cut(s) 488, 506, 1100
Tsp45I GTSAC 4 cut(s) 676, 688, 886, 947
TspDTI ATGAA 6 cut(s) 17, 65, 551, 608, 1080, 1126
TspGWI ACGGA 1 cut(s) 1149
TspRI CASTG 2 cut(s) 337, 697
Tth111I GACNNNGTC 2 cut(s) 88, 100
Vha464I CTTAAG 1 cut(s) 865
VpaK11BI GGWCC 2 cut(s) 155, 896
XceI RCATGY 4 cut(s) 274, 352, 1028, 1100
XspI CTAG 2 cut(s) 380, 468
ZraI GACGTC 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.