RLG00000031336

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000394
Physical Location & Seq
Forward (+)
4201812 .. 4203314
1503 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031336

Sequence Viewer

Length: 564 bp
ATGAAGGACATAGTGGTTAGCTCACCAGGCGGACTGGAGGTCCTCCAAGTACAGGAAGTGGAAGACCCCAAAAATAAAAGACGACGAGTTTCTAGTCAAAGTCGAGGCTACGTCACTGAACCAGGCAGACAGCTTACAGCAGGCCGTCGGGGAACACGTGTCACGCTGGAAAGCCGGCGATCAGCAATGCATATATATTGGATTCACAACAGCTTGAGGAGGAAACACAGTAGACTTGCGTTAGTACAGGTATGGGATCGAGAGATTTTAATTCAACCTCTGCATGATTATTACACGGACATGGAACTCCTCCTAGCCTGCAAGACATACAGGGAAACATCACAGATGGAGTCTGGTCTGATGATGTTACATCTGGGGACATTCGTTGTATCTGCATTTTCATATTCTATTATTGTTGGTGAACTTGTCTACAAGGCCATTCTGTCCAGTGATACTCTAACAGGATTACTTCCTCCCTCGGGTACCTATTGTGGACTGCTGAAGGTGGAGTCCGGTTGGCTGCTGTCCTCAATTGCGCTTCTAAATCTGATGCACAACTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

188

Amino Acids

21.17

Weight (kDa)

9.22

Isoelectric Point (pI)

68.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000394)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21580
fragaria_vesca FvH4_3g02190 FvH4_3g02190 FvH4_3g02190 FvH4_3g04320 FvH4_3g04340 FvH4_3g18080 FvH4_3g18080 FvH4_3g18080 FvH4_6g30490 FvH4_6g30490 FvH4_6g30490 FvH4_6g30490
malus_domestica MD05G1217800.v1.1 MD05G1323900.v1.1 MD12G1074500.v1.1 MD14G1066500.v1.1
prunus_persica Prupe.4G038700_v2.0.a1 Prupe.7G059000_v2.0.a1 Prupe.7G059000_v2.0.a1 Prupe.7G059300_v2.0.a1 Prupe.7G059300_v2.0.a1 Prupe.7G059300_v2.0.a1 Prupe.7G059300_v2.0.a1
pyrus_communis pycom05g29820
rosa_chinensis RchiOBHm_Chr1g0318171 RchiOBHm_Chr5g0003241 RchiOBHm_Chr5g0006701 RchiOBHm_Chr5g0006721 RchiOBHm_Chr5g0006731 RchiOBHm_Chr5g0030211 RchiOBHm_Chr5g0030291 RchiOBHm_Chr5g0030331 RchiOBHm_Chr5g0030371 RchiOBHm_Chr5g0030401 RchiOBHm_Chr5g0030471
rosa_laevigata RLG00000025923 RLG00000025924 RLG00000030556 RLG00000031088 RLG00000031336 RLG00000031418 RLG00000031420 RLG00000031421 RLG00000031422 RLG00000031423 RLG00000033220
rosa_multiflora Rmu_sc0001886.1_g000010 Rmu_sc0001886.1_g000011 Rmu_sc0001886.1_g000012 Rmu_sc0010917.1_g000018 Rmu_sc0011424.1_g000013 Rmu_sc0017347.1_g000003 Rmu_sc0017347.1_g000005 Rmu_sc0018387.1_g000006 Rmu_sc0018387.1_g000008
rosa_roxburghii Rroxscaffold_1G00049810 Rroxscaffold_1G00069320 Rroxscaffold_1G00072770 Rroxscaffold_4G00329990 Rroxscaffold_6G00426520
rosa_rugosa Rorug01G0019200 Rorug01G0019300 Rorug01G0019400 Rorug02G0611300 Rorug02G0611400 Rorug04G0401400 Rorug04G0423700 Rorug04G0424000 Rorug05G0118000
rosa_samantha Rh1CG030000 Rh1DG040100 Rh2DG133300 Rh3AG010300 Rh3BG010000 Rh3DG010500 Rh5AG026800 Rh5AG026900 Rh5AG027800 Rh5AG051600 Rh5AG051800 Rh5BG028000 Rh5BG050700 Rh5BG129800 Rh5BG129900 Rh5CG029400 Rh5CG029500 Rh5CG030100 Rh5CG059100 Rh5CG059300 Rh5CG059400 Rh5DG026200 Rh5DG049800 Rh5DG049900 Rh5DG214000
rosa_wichuraiana Rw1G002510 Rw5G002450 Rw5G002640 Rw5G004650 Rw5G005260 Rw5G005270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 482
AccB1I GGYRCC 1 cut(s) 482
AccI GTMKAC 2 cut(s) 232, 429
AciI CCGC 1 cut(s) 30
AclWI GGATC 1 cut(s) 264
AcuI CTGAAG 1 cut(s) 521
AcvI CACGTG 1 cut(s) 158
AfaI GTAC 3 cut(s) 51, 246, 484
AfiI CCNNNNNNNGG 2 cut(s) 52, 479
AflIII ACRYGT 2 cut(s) 155, 157
AgsI TTSAA 1 cut(s) 275
AhdI GACNNNNNGTC 1 cut(s) 38
AjnI CCWGG 2 cut(s) 25, 121
AluBI AGCT 3 cut(s) 21, 133, 213
AluI AGCT 3 cut(s) 21, 133, 213
AlwI GGATC 1 cut(s) 264
Ama87I CYCGRG 1 cut(s) 478
AoxI GGCC 2 cut(s) 142, 435
ApeKI GCWGC 1 cut(s) 520
Asp718I GGTACC 1 cut(s) 482
AspLEI GCGC 1 cut(s) 538
AspS9I GGNCC 1 cut(s) 40
AsuHPI GGTGA 2 cut(s) 15, 431
AvaI CYCGRG 1 cut(s) 478
AvaII GGWCC 1 cut(s) 40
BanI GGYRCC 1 cut(s) 482
BbrPI CACGTG 1 cut(s) 158
BbsI GAAGAC 1 cut(s) 69
BbvI GCAGC 1 cut(s) 507
BccI CCATC 1 cut(s) 340
BceAI ACGGC 1 cut(s) 129
BciT130I CCWGG 2 cut(s) 27, 123
BfaI CTAG 2 cut(s) 93, 314
BisI GCNGC 1 cut(s) 521
BlsI GCNGC 1 cut(s) 522
Bme1390I CCNGG 2 cut(s) 27, 123
Bme18I GGWCC 1 cut(s) 40
BmeRI GACNNNNNGTC 1 cut(s) 38
BmeT110I CYCGRG 1 cut(s) 478
BmgT120I GGNCC 1 cut(s) 40
BmiI GGNNCC 1 cut(s) 484
BmrFI CCNGG 2 cut(s) 27, 123
BmsI GCATC 1 cut(s) 540
BpiI GAAGAC 1 cut(s) 69
BpmI CTGGAG 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 235
BsaAI YACGTR 1 cut(s) 158
BsaJI CCNNGG 1 cut(s) 477
BsaWI WCCGGW 1 cut(s) 512
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 479
Bse118I RCCGGY 1 cut(s) 174
Bse1I ACTGG 2 cut(s) 39, 447
Bse3DI GCAATG 1 cut(s) 192
BseBI CCWGG 2 cut(s) 27, 123
BseDI CCNNGG 1 cut(s) 477
BseLI CCNNNNNNNGG 2 cut(s) 52, 479
BseMI GCAATG 1 cut(s) 192
BseNI ACTGG 2 cut(s) 39, 447
BseRI GAGGAG 2 cut(s) 232, 299
BseXI GCAGC 1 cut(s) 507
BshFI GGCC 2 cut(s) 144, 437
BshNI GGYRCC 1 cut(s) 482
BsiHKCI CYCGRG 1 cut(s) 478
BsiSI CCGG 2 cut(s) 175, 513
BslFI GGGAC 1 cut(s) 391
BslI CCNNNNNNNGG 2 cut(s) 52, 479
BsmFI GGGAC 1 cut(s) 391
BsnI GGCC 2 cut(s) 144, 437
BsoBI CYCGRG 1 cut(s) 478
Bsp143I GATC 2 cut(s) 179, 256
BspACI CCGC 1 cut(s) 30
BspANI GGCC 2 cut(s) 144, 437
BspLI GGNNCC 1 cut(s) 484
BspPI GGATC 1 cut(s) 264
BspT107I GGYRCC 1 cut(s) 482
BsrDI GCAATG 1 cut(s) 192
BsrFI RCCGGY 1 cut(s) 174
BsrI ACTGG 2 cut(s) 39, 447
BssAI RCCGGY 1 cut(s) 174
BssECI CCNNGG 1 cut(s) 477
BssMI GATC 2 cut(s) 179, 256
Bst2UI CCWGG 2 cut(s) 27, 123
Bst4CI ACNGT 1 cut(s) 230
BstBAI YACGTR 1 cut(s) 158
BstC8I GCNNGC 3 cut(s) 142, 176, 319
BstHHI GCGC 1 cut(s) 538
BstKTI GATC 2 cut(s) 182, 259
BstMBI GATC 2 cut(s) 179, 256
BstMWI GCNNNNNNNGC 1 cut(s) 27
BstNI CCWGG 2 cut(s) 27, 123
BstSCI CCNGG 2 cut(s) 25, 121
BstV1I GCAGC 1 cut(s) 507
BstV2I GAAGAC 1 cut(s) 69
BsuRI GGCC 2 cut(s) 144, 437
BtsIMutI CAGTG 2 cut(s) 114, 454
Cac8I GCNNGC 3 cut(s) 142, 176, 319
CfoI GCGC 1 cut(s) 538
Cfr10I RCCGGY 1 cut(s) 174
Cfr13I GGNCC 1 cut(s) 40
Csp6I GTAC 3 cut(s) 50, 245, 483
CviAII CATG 2 cut(s) 284, 301
CviJI RGCY 9 cut(s) 21, 108, 133, 144, 174, 213, 317, 437, 520
CviKI_1 RGCY 9 cut(s) 21, 108, 133, 144, 174, 213, 317, 437, 520
CviQI GTAC 3 cut(s) 50, 245, 483
DpnI GATC 2 cut(s) 181, 258
DpnII GATC 2 cut(s) 179, 256
DriI GACNNNNNGTC 1 cut(s) 38
Eam1105I GACNNNNNGTC 1 cut(s) 38
EciI GGCGGA 1 cut(s) 45
Eco47I GGWCC 1 cut(s) 40
Eco57I CTGAAG 1 cut(s) 521
Eco72I CACGTG 1 cut(s) 158
Eco88I CYCGRG 1 cut(s) 478
EcoO109I RGGNCCY 1 cut(s) 40
EcoRII CCWGG 2 cut(s) 25, 121
EcoT22I ATGCAT 1 cut(s) 192
FaeI CATG 2 cut(s) 287, 304
FaiI YATR 9 cut(s) 11, 192, 194, 196, 253, 285, 302, 328, 403
FaqI GGGAC 1 cut(s) 391
FatI CATG 2 cut(s) 283, 300
FblI GTMKAC 2 cut(s) 232, 429
Fnu4HI GCNGC 1 cut(s) 521
Fsp4HI GCNGC 1 cut(s) 521
FspBI CTAG 2 cut(s) 93, 314
GlaI GCGC 1 cut(s) 537
GluI GCNGC 1 cut(s) 521
GsuI CTGGAG 1 cut(s) 56
HaeIII GGCC 2 cut(s) 144, 437
HapII CCGG 2 cut(s) 175, 513
HhaI GCGC 1 cut(s) 538
Hin1II CATG 2 cut(s) 287, 304
Hin6I GCGC 1 cut(s) 536
HinP1I GCGC 1 cut(s) 536
HinfI GANTC 3 cut(s) 202, 350, 509
HpaII CCGG 2 cut(s) 175, 513
HphI GGTGA 2 cut(s) 15, 431
Hpy166II GTNNAC 4 cut(s) 233, 422, 430, 494
Hpy188I TCNGA 2 cut(s) 360, 549
Hpy188III TCNNGA 1 cut(s) 260
Hpy8I GTNNAC 4 cut(s) 233, 422, 430, 494
Hpy99I CGWCG 2 cut(s) 87, 150
HpyAV CCTTC 1 cut(s) 496
HpyCH4III ACNGT 1 cut(s) 230
HpyCH4IV ACGT 2 cut(s) 111, 157
HpyCH4V TGCA 5 cut(s) 190, 283, 321, 395, 553
HpyF10VI GCNNNNNNNGC 1 cut(s) 27
HpySE526I ACGT 2 cut(s) 111, 157
Hsp92II CATG 2 cut(s) 287, 304
HspAI GCGC 1 cut(s) 536
KpnI GGTACC 1 cut(s) 486
KroI GCCGGC 1 cut(s) 174
KroNI GCCGGC 1 cut(s) 176
Kzo9I GATC 2 cut(s) 179, 256
Lsp1109I GCAGC 1 cut(s) 507
LweI GCATC 1 cut(s) 540
MaeI CTAG 2 cut(s) 93, 314
MaeII ACGT 2 cut(s) 111, 157
MaeIII GTNAC 3 cut(s) 112, 160, 366
MalI GATC 2 cut(s) 181, 258
MboI GATC 2 cut(s) 179, 256
MboII GAAGA 1 cut(s) 74
MfeI CAATTG 1 cut(s) 531
MluCI AATT 2 cut(s) 270, 531
MlyI GAGTC 2 cut(s) 359, 518
Mph1103I ATGCAT 1 cut(s) 192
MroNI GCCGGC 1 cut(s) 174
MseI TTAA 1 cut(s) 269
MslI CAYNNNNRTG 1 cut(s) 299
MspI CCGG 2 cut(s) 175, 513
MspR9I CCNGG 2 cut(s) 27, 123
MunI CAATTG 1 cut(s) 531
MvaI CCWGG 2 cut(s) 27, 123
MwoI GCNNNNNNNGC 1 cut(s) 27
NaeI GCCGGC 1 cut(s) 176
NdeII GATC 2 cut(s) 179, 256
NgoMIV GCCGGC 1 cut(s) 174
NlaIII CATG 2 cut(s) 287, 304
NlaIV GGNNCC 1 cut(s) 484
NmuCI GTSAC 2 cut(s) 112, 160
NsiI ATGCAT 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 154
PdiI GCCGGC 1 cut(s) 176
PfeI GAWTC 1 cut(s) 202
PkrI GCNGC 1 cut(s) 522
PleI GAGTC 2 cut(s) 358, 517
PmaCI CACGTG 1 cut(s) 158
PmlI CACGTG 1 cut(s) 158
PpsI GAGTC 2 cut(s) 358, 517
Ppu21I YACGTR 1 cut(s) 158
PpuMI RGGWCCY 1 cut(s) 40
Psp5II RGGWCCY 1 cut(s) 40
Psp6I CCWGG 2 cut(s) 25, 121
PspCI CACGTG 1 cut(s) 158
PspGI CCWGG 2 cut(s) 25, 121
PspN4I GGNNCC 1 cut(s) 484
PspPI GGNCC 1 cut(s) 40
PspPPI RGGWCCY 1 cut(s) 40
RsaI GTAC 3 cut(s) 51, 246, 484
RsaNI GTAC 3 cut(s) 50, 245, 483
RseI CAYNNNNRTG 1 cut(s) 299
SaqAI TTAA 1 cut(s) 269
SatI GCNGC 1 cut(s) 521
Sau3AI GATC 2 cut(s) 179, 256
Sau96I GGNCC 1 cut(s) 40
SchI GAGTC 2 cut(s) 359, 518
ScrFI CCNGG 2 cut(s) 27, 123
SfaNI GCATC 1 cut(s) 540
SinI GGWCC 1 cut(s) 40
SmiMI CAYNNNNRTG 1 cut(s) 299
SmlI CTYRAG 1 cut(s) 214
SmoI CTYRAG 1 cut(s) 214
Sse9I AATT 2 cut(s) 270, 531
SsiI CCGC 1 cut(s) 30
SspMI CTAG 2 cut(s) 93, 314
StyD4I CCNGG 2 cut(s) 25, 121
TaaI ACNGT 1 cut(s) 230
TaiI ACGT 2 cut(s) 114, 160
TaqI TCGA 2 cut(s) 103, 259
TasI AATT 2 cut(s) 270, 531
TatI WGTACW 2 cut(s) 49, 244
TfiI GAWTC 1 cut(s) 202
Tru1I TTAA 1 cut(s) 269
Tru9I TTAA 1 cut(s) 269
TscAI CASTG 2 cut(s) 121, 454
TseFI GTSAC 2 cut(s) 112, 160
TseI GCWGC 1 cut(s) 520
Tsp45I GTSAC 2 cut(s) 112, 160
TspDTI ATGAA 2 cut(s) 17, 390
TspGWI ACGGA 1 cut(s) 311
TspRI CASTG 2 cut(s) 121, 454
VpaK11BI GGWCC 1 cut(s) 40
XmiI GTMKAC 2 cut(s) 232, 429
XspI CTAG 2 cut(s) 93, 314
Zsp2I ATGCAT 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.