Rw5G005080

Galactose binding lectin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0016929
Physical Location & Seq
Forward (+)
5292278 .. 5297022
4745 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G005080.1

Sequence Viewer

Length: 2427 bp
ATGGGTAGCAAAAGTTGGGTGCCATTGGTTCTGTTTGCGATCATAGTTTTTGAGGGTTATTTCATTGAACCAGTAAATTCTGCCACTGTGACATATGACCATAGAGCACTGGTTATTGATGGGAAGCGGCGGGTTTTGCAGTCTATCCATTATCCTAGAACCAACCCTGAGGTGTGGCCGGAGATTATTAATAAGGCCAAGGAAGGAGGACTTGACGTGATAGAGAGTTATGTTTTCTGGAACTATCATGAGCCTGTAAAAGGCCAGTACTACTTTGAAGGCAGGTTTGATTTGGTGAGATTTGTGAAGACAGTGCAAGAAGCTGGCCTTCTAGTTCATTTGAGGATTGGCCCTTATGCTTGTGCTGAATGGAACTACGGGGGATTTCCTATTTGGTTACACTTTATTCCCGGAATTCAATTCCGTACTACAAATGCACCTTTTAAGAATGAAATGAAGCACTTTCTTGCAAAAATTGTTGAAATGATGAAGGAGGAGCATCTCTTTGCATCACAAGGGGGGCCGATCATTCTTGCTCAGGTTGAAAATGAATATGGAAATGTCGAAGGGTCATATGGAGTTGGTGGAGAATTATATGTCAAATGGGCTGCAGAAACTGCTATAAACACATGCAATGGATTTTATTGTGATATGTTTGCCCCGAATTCTCCAAATAAACCGAAGATGTGGACAGAGAATTACAGTGGATGGTTCCTTGGATTCGGCAATCCAATTCCTTATAGACCTGTTGAGGATCTTGCTTTCGCTGTTGCACGGTTTTTTGAAACAGGTGGTACATTTCAAAACTATTATATGTATTTTGGTGGGACCAACTTCGGGAGAACAGCTGGAGGCCCTTTGGTTGCAACAAGCTATGATTATGATGCTCCTATAGATGAATATGGTTTTCTTAGACAGCCAAAATGGGGCCACCTGCGTGATTTGCACAAGGCAATAAAGCACTGTGAAAAGTATCTGATCAGCTCTGATCCCACTCGAGTGCAACTTGGAGTAAATTTAGAGGCGCATATCTACTATAAACATTCCAATGAGTGTGCAGCCTTTCTTGCCAATTATGGAAGTAGATCAGATGCAAATGTCACATTCAACGGACATGTTTATTTCCTTCCAGCTTGGTCTGTAAGCATCCTTCCAGATTGTAAGAATGTTATATTCAACACCGCCAAGGTTGTCTCACAAAAAACTCTTGGAGATCCTCTTTTTGCCAGCACAACAATGAAACCATCTTCATGGGGATGGTACAAAGAAAGAGTTGGTATTTGGAGTAACAGTTCATTTAGTAGCCCAGGCTTACTGGAGCAGATAAGTACCACGAAAGATACTAGTGATTTTTTATGGTACTCAATAAGTATCAATGTGAATGAAGACATTGCACCTGGTCAAGGGAAAGAACTGTTTTTAAATATTGAGAGCCTGGGACACGCCGCTCTAGTTTTTGTAAACAAAAAGCCTATAGGATTTGGATATGGTAACCATGAAGATGCTAGCTTCATCCTTAATGAGAAGGTTGCTCTGAACCCTGGAAACAATACCATTGATATGTTGAGTATGATGATTGGTGTACAGAACTATGGACCGTGGTTCGATGTTGCAGGAGCAGGAATTTTCTTTGTTGTTATTGCTGACTTAGAGACTGGAAAAGTTGATCTTTCTGATGAAGAATGGACCTACCAGGTAGGAGTGGAAGGGGAATACCTTGGATTGGATAACATAAGTCTCGCAAATAGTTCACTGTGGACTACAGGGGCTACTCCACCAGTTAATCAGACTTTGATATGGTACAAGGTTTCATTCCTTGCTCCTGAAGGAAAGGGTCCTTTGGCATTAGATCTTGCTAGCATGGGAAAGGGGCAGGCCTGGGTTAATGGGCAGGGCATAGGGAGATATTGGTCCACGTATCTCTCACCATCTACTGGTTGCAACAGTAGCGGTGTTTGCGACTACAGGGGAGCCTATGATGCAAATAAATGTCTAAAGAACTGTGGTCAACCAGCTCAAACTCTGTACCATATTCCTCGTACTTGGGTACAAGATGGAGAGAACCTTCTAGTTCTCCATGAAGAGCTTGGGGGTGACCCTTCAAAAATCTCTGTGAGGACACGAACTGGCCAGAGATATGTGCGTACTTCTTGGAAACCAAACTCTGAATTCATGTCCCAAAATCCTGAAGTTCAACTGACCTGTGAACATGGATGGCACATTACTTCAATTAACTTTGCTAGCTATGGAACTCCTATCGGAAGCTGTGGTGCCACTTTCACTCTGGGAACTTGCAATGCTGATGTCTTAACAATCGTTGAGCAGGTAAGAGGAATCTTCTCTTCTTTCTATCTTATAATAACCTTAGGTGATCCTTGTCCAGGTGTATTAAAGAGTTTAGCTGTTCAAGCTCTTTGCAGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gal_Lectin Glyco_hydro_35

Protein Analysis

808

Amino Acids

89.96

Weight (kDa)

5.67

Isoelectric Point (pI)

32.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 37 - 209 5.3e-66 Glycosyl hydrolases family 35
Glyco_hydro_42 PF02449 55 - 192 3.9e-06 Beta-galactosidase
Glyco_hydro_35 PF01301 205 - 318 1.5e-33 Glycosyl hydrolases family 35
GHD PF17834 341 - 397 1.7e-25 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 598 - 688 2.2e-21 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 733 - 806 9.1e-06 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016929)

Species Orthologous Gene IDs
malus_domestica MD10G1301600.v1.1
prunus_persica Prupe.4G038600_v2.0.a1
pyrus_communis pycom10g25440
rosa_chinensis RchiOBHm_Chr5g0006691
rosa_laevigata RLG00000031417 RLG00000031511
rosa_multiflora Rmu_sc0017347.1_g000002 Rmu_sc0018387.1_g000009
rosa_roxburghii Rroxscaffold_1G00069330
rosa_rugosa Rorug04G0423700
rosa_samantha Rh5AG051500 Rh5CG059000
rosa_wichuraiana Rw5G005080 Rw5G005300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2357
AarI CACCTGC 1 cut(s) 942
Acc36I ACCTGC 3 cut(s) 273, 942, 2314
AccB1I GGYRCC 2 cut(s) 19, 2270
AccB7I CCANNNNNTGG 1 cut(s) 1934
AccBSI CCGCTC 1 cut(s) 1448
AciI CCGC 5 cut(s) 127, 130, 1182, 1446, 1950
AclWI GGATC 4 cut(s) 762, 983, 1208, 2366
AcoI YGGCCR 2 cut(s) 176, 2128
AcsI RAATTY 6 cut(s) 76, 414, 664, 1015, 1623, 2168
AcuI CTGAAG 2 cut(s) 1845, 2208
AfiI CCNNNNNNNGG 7 cut(s) 260, 837, 926, 1403, 1723, 1934, 2381
AflIII ACRYGT 1 cut(s) 1114
AhlI ACTAGT 1 cut(s) 1343
AjiI CACGTC 1 cut(s) 217
AjnI CCWGG 7 cut(s) 1306, 1396, 1434, 1540, 1692, 1877, 2380
AleI CACNNNNGTG 2 cut(s) 936, 998
AloI GAACNNNNNNTCC 2 cut(s) 1276, 1308
Alw21I GWGCWC 1 cut(s) 109
Alw26I GTCTC 3 cut(s) 1198, 1646, 1742
AlwI GGATC 4 cut(s) 762, 983, 1208, 2366
AlwNI CAGNNNCTG 1 cut(s) 617
Ama87I CYCGRG 1 cut(s) 996
ApeKI GCWGC 3 cut(s) 608, 1058, 2418
ApoI RAATTY 6 cut(s) 76, 414, 664, 1015, 1623, 2168
AseI ATTAAT 1 cut(s) 189
AspLEI GCGC 1 cut(s) 1027
AspS9I GGNCC 9 cut(s) 350, 521, 828, 854, 928, 1595, 1686, 1835, 1911
AsuC2I CCSGG 1 cut(s) 411
AsuHPI GGTGA 4 cut(s) 307, 1917, 2105, 2381
AsuNHI GCTAGC 3 cut(s) 1505, 1856, 2240
AvaI CYCGRG 1 cut(s) 996
AvaII GGWCC 5 cut(s) 828, 1595, 1686, 1835, 1911
AxyI CCTNAGG 2 cut(s) 168, 2365
BalI TGGCCA 1 cut(s) 2130
BanI GGYRCC 2 cut(s) 19, 2270
BarI GAAGNNNNNNTAC 2 cut(s) 1134, 1166
BbsI GAAGAC 2 cut(s) 314, 1392
Bbv12I GWGCWC 1 cut(s) 109
BbvI GCAGC 2 cut(s) 595, 1070
BccI CCATC 7 cut(s) 113, 702, 1251, 1252, 1936, 2048, 2208
BciT130I CCWGG 7 cut(s) 1308, 1398, 1436, 1542, 1694, 1879, 2382
BclI TGATCA 1 cut(s) 978
BcnI CCSGG 1 cut(s) 411
BcoDI GTCTC 3 cut(s) 1198, 1646, 1742
BcuI ACTAGT 1 cut(s) 1343
BfaI CTAG 8 cut(s) 156, 332, 1344, 1451, 1506, 1857, 2069, 2241
BfmI CTRYAG 5 cut(s) 609, 891, 1473, 1761, 1963
BfuAI ACCTGC 3 cut(s) 273, 942, 2314
BglII AGATCT 1 cut(s) 1849
BisI GCNGC 5 cut(s) 128, 609, 1059, 1446, 2419
BlsI GCNGC 5 cut(s) 129, 610, 1060, 1447, 2420
BmcAI AGTACT 1 cut(s) 269
Bme1390I CCNGG 8 cut(s) 411, 1308, 1398, 1436, 1542, 1694, 1879, 2382
Bme18I GGWCC 5 cut(s) 828, 1595, 1686, 1835, 1911
BmeT110I CYCGRG 1 cut(s) 996
BmgBI CACGTC 1 cut(s) 217
BmgT120I GGNCC 9 cut(s) 350, 521, 828, 854, 928, 1595, 1686, 1835, 1911
BmiI GGNNCC 8 cut(s) 21, 522, 713, 829, 929, 1836, 1972, 2272
BmrFI CCNGG 8 cut(s) 411, 1308, 1398, 1436, 1542, 1694, 1879, 2382
BmsI GCATC 7 cut(s) 508, 518, 874, 1081, 1155, 1492, 1969
BmtI GCTAGC 3 cut(s) 1509, 1860, 2244
BpiI GAAGAC 2 cut(s) 314, 1392
BpmI CTGGAG 2 cut(s) 870, 1337
Bpu10I CCTNAGC 1 cut(s) 537
BpuMI CCSGG 1 cut(s) 411
BsaAI YACGTR 1 cut(s) 1917
BsaJI CCNNGG 9 cut(s) 198, 715, 1185, 1306, 1435, 1540, 1598, 1717, 1878
BsaXI ACNNNNNCTCC 2 cut(s) 1276, 1306
Bsc4I CCNNNNNNNGG 7 cut(s) 260, 837, 926, 1403, 1723, 1934, 2381
Bse1I ACTGG 8 cut(s) 71, 114, 265, 1320, 1660, 1778, 1939, 2131
Bse21I CCTNAGG 2 cut(s) 168, 2365
Bse3DI GCAATG 3 cut(s) 640, 1389, 2302
BseBI CCWGG 7 cut(s) 1308, 1398, 1436, 1542, 1694, 1879, 2382
BseDI CCNNGG 9 cut(s) 198, 715, 1185, 1306, 1435, 1540, 1598, 1717, 1878
BseGI GGATG 5 cut(s) 713, 1146, 1262, 1512, 2219
BseLI CCNNNNNNNGG 7 cut(s) 260, 837, 926, 1403, 1723, 1934, 2381
BseMI GCAATG 3 cut(s) 640, 1389, 2302
BseMII CTCAG 2 cut(s) 159, 551
BseNI ACTGG 8 cut(s) 71, 114, 265, 1320, 1660, 1778, 1939, 2131
BseRI GAGGAG 1 cut(s) 509
BseXI GCAGC 2 cut(s) 595, 1070
BsgI GTGCAG 1 cut(s) 1077
BshNI GGYRCC 2 cut(s) 19, 2270
BsiHKAI GWGCWC 1 cut(s) 109
BsiHKCI CYCGRG 1 cut(s) 996
BsiSI CCGG 2 cut(s) 179, 411
BslFI GGGAC 3 cut(s) 841, 1452, 2161
BslI CCNNNNNNNGG 7 cut(s) 260, 837, 926, 1403, 1723, 1934, 2381
BsmAI GTCTC 3 cut(s) 1198, 1646, 1742
BsmFI GGGAC 3 cut(s) 841, 1452, 2161
BsoBI CYCGRG 1 cut(s) 996
Bsp1286I GDGCHC 1 cut(s) 109
Bsp1407I TGTACA 1 cut(s) 1582
BspACI CCGC 5 cut(s) 127, 130, 1182, 1446, 1950
BspCNI CTCAG 2 cut(s) 160, 550
BspHI TCATGA 1 cut(s) 247
BspLI GGNNCC 8 cut(s) 21, 522, 713, 829, 929, 1836, 1972, 2272
BspMAI CTGCAG 1 cut(s) 613
BspMI ACCTGC 3 cut(s) 273, 942, 2314
BspOI GCTAGC 3 cut(s) 1509, 1860, 2244
BspPI GGATC 4 cut(s) 762, 983, 1208, 2366
BspQI GCTCTTC 1 cut(s) 2076
BspT107I GGYRCC 2 cut(s) 19, 2270
BsrBI CCGCTC 1 cut(s) 1448
BsrDI GCAATG 3 cut(s) 640, 1389, 2302
BsrGI TGTACA 1 cut(s) 1582
BsrI ACTGG 8 cut(s) 71, 114, 265, 1320, 1660, 1778, 1939, 2131
BssECI CCNNGG 9 cut(s) 198, 715, 1185, 1306, 1435, 1540, 1598, 1717, 1878
BssT1I CCWWGG 4 cut(s) 198, 715, 1185, 1717
Bst2UI CCWGG 7 cut(s) 1308, 1398, 1436, 1542, 1694, 1879, 2382
Bst6I CTCTTC 2 cut(s) 2076, 2347
BstAPI GCANNNNNTGC 1 cut(s) 617
BstAUI TGTACA 1 cut(s) 1582
BstBAI YACGTR 1 cut(s) 1917
BstC8I GCNNGC 6 cut(s) 325, 1228, 1507, 1858, 1875, 2242
BstDEI CTNAG 5 cut(s) 168, 537, 911, 1648, 2365
BstDSI CCRYGG 1 cut(s) 1598
BstEII GGTNACC 2 cut(s) 1490, 2093
BstENI CCTNNNNNAGG 3 cut(s) 258, 1401, 2379
BstF5I GGATG 5 cut(s) 713, 1146, 1262, 1512, 2219
BstHHI GCGC 1 cut(s) 1027
BstMAI GTCTC 3 cut(s) 1198, 1646, 1742
BstMWI GCNNNNNNNGC 8 cut(s) 136, 617, 943, 1067, 1947, 1956, 1979, 2408
BstNI CCWGG 7 cut(s) 1308, 1398, 1436, 1542, 1694, 1879, 2382
BstNSI RCATGY 2 cut(s) 633, 1118
BstPI GGTNACC 2 cut(s) 1490, 2093
BstSCI CCNGG 8 cut(s) 409, 1306, 1396, 1434, 1540, 1692, 1877, 2380
BstSFI CTRYAG 5 cut(s) 609, 891, 1473, 1761, 1963
BstV1I GCAGC 2 cut(s) 595, 1070
BstV2I GAAGAC 2 cut(s) 314, 1392
BstX2I RGATCY 3 cut(s) 754, 1213, 1849
BstXI CCANNNNNNTGG 1 cut(s) 1251
BstYI RGATCY 3 cut(s) 754, 1213, 1849
Bsu36I CCTNAGG 2 cut(s) 168, 2365
BtgI CCRYGG 1 cut(s) 1598
BtrI CACGTC 1 cut(s) 217
BtsCI GGATG 5 cut(s) 713, 1146, 1262, 1512, 2219
BtsIMutI CAGTG 6 cut(s) 84, 107, 318, 709, 961, 1751
BveI ACCTGC 3 cut(s) 273, 942, 2314
Cac8I GCNNGC 6 cut(s) 325, 1228, 1507, 1858, 1875, 2242
CaiI CAGNNNCTG 1 cut(s) 617
CciI TCATGA 1 cut(s) 247
CfoI GCGC 1 cut(s) 1027
Cfr13I GGNCC 9 cut(s) 350, 521, 828, 854, 928, 1595, 1686, 1835, 1911
CsiI ACCWGGT 2 cut(s) 1396, 1692
CviAII CATG 9 cut(s) 248, 630, 1115, 1251, 1496, 1861, 2078, 2173, 2210
DdeI CTNAG 5 cut(s) 168, 537, 911, 1648, 2365
DraI TTTAAA 1 cut(s) 1422
EaeI YGGCCR 2 cut(s) 176, 2128
Eam1104I CTCTTC 2 cut(s) 2076, 2347
EarI CTCTTC 2 cut(s) 2076, 2347
Eco130I CCWWGG 4 cut(s) 198, 715, 1185, 1717
Eco147I AGGCCT 1 cut(s) 1877
Eco47I GGWCC 5 cut(s) 828, 1595, 1686, 1835, 1911
Eco57I CTGAAG 2 cut(s) 1845, 2208
Eco81I CCTNAGG 2 cut(s) 168, 2365
Eco88I CYCGRG 1 cut(s) 996
Eco91I GGTNACC 2 cut(s) 1490, 2093
EcoNI CCTNNNNNAGG 3 cut(s) 258, 1401, 2379
EcoO109I RGGNCCY 2 cut(s) 854, 1835
EcoO65I GGTNACC 2 cut(s) 1490, 2093
EcoRI GAATTC 3 cut(s) 414, 664, 2168
EcoRII CCWGG 7 cut(s) 1306, 1396, 1434, 1540, 1692, 1877, 2380
EcoT14I CCWWGG 4 cut(s) 198, 715, 1185, 1717
ErhI CCWWGG 4 cut(s) 198, 715, 1185, 1717
FaeI CATG 9 cut(s) 251, 633, 1118, 1254, 1499, 1864, 2081, 2176, 2213
FalI AAGNNNNNCTT 6 cut(s) 195, 227, 312, 344, 1653, 1685
FaqI GGGAC 3 cut(s) 841, 1452, 2161
FatI CATG 9 cut(s) 247, 629, 1114, 1250, 1495, 1860, 2077, 2172, 2209
FauI CCCGC 1 cut(s) 123
FauNDI CATATG 2 cut(s) 94, 574
FbaI TGATCA 1 cut(s) 978
Fnu4HI GCNGC 5 cut(s) 128, 609, 1059, 1446, 2419
FokI GGATG 5 cut(s) 720, 1133, 1269, 1499, 2226
Fsp4HI GCNGC 5 cut(s) 128, 609, 1059, 1446, 2419
FspBI CTAG 8 cut(s) 156, 332, 1344, 1451, 1506, 1857, 2069, 2241
GlaI GCGC 1 cut(s) 1026
GluI GCNGC 5 cut(s) 128, 609, 1059, 1446, 2419
GsuI CTGGAG 2 cut(s) 870, 1337
HapII CCGG 2 cut(s) 179, 411
HhaI GCGC 1 cut(s) 1027
Hin1II CATG 9 cut(s) 251, 633, 1118, 1254, 1499, 1864, 2081, 2176, 2213
Hin6I GCGC 1 cut(s) 1025
HinP1I GCGC 1 cut(s) 1025
HincII GTYRAC 1 cut(s) 2009
HindII GTYRAC 1 cut(s) 2009
HinfI GANTC 2 cut(s) 720, 2334
HpaII CCGG 2 cut(s) 179, 411
HphI GGTGA 4 cut(s) 307, 1917, 2105, 2381
Hpy166II GTNNAC 8 cut(s) 690, 1462, 1583, 1751, 1758, 1914, 2009, 2207
Hpy188I TCNGA 8 cut(s) 978, 988, 1090, 1536, 1675, 1788, 2167, 2261
Hpy188III TCNNGA 6 cut(s) 238, 248, 838, 1154, 1823, 2186
Hpy8I GTNNAC 8 cut(s) 690, 1462, 1583, 1751, 1758, 1914, 2009, 2207
HpyCH4IV ACGT 2 cut(s) 216, 1916
HpyF10VI GCNNNNNNNGC 8 cut(s) 136, 617, 943, 1067, 1947, 1956, 1979, 2408
HpyF3I CTNAG 5 cut(s) 168, 537, 911, 1648, 2365
HpySE526I ACGT 2 cut(s) 216, 1916
Hsp92II CATG 9 cut(s) 251, 633, 1118, 1254, 1499, 1864, 2081, 2176, 2213
HspAI GCGC 1 cut(s) 1025
Ksp22I TGATCA 1 cut(s) 978
LguI GCTCTTC 1 cut(s) 2076
LmnI GCTCC 6 cut(s) 496, 892, 1318, 1616, 1825, 1970
Lsp1109I GCAGC 2 cut(s) 595, 1070
LweI GCATC 7 cut(s) 508, 518, 874, 1081, 1155, 1492, 1969
MabI ACCWGGT 2 cut(s) 1396, 1692
MaeI CTAG 8 cut(s) 156, 332, 1344, 1451, 1506, 1857, 2069, 2241
MaeII ACGT 2 cut(s) 216, 1916
MaeIII GTNAC 6 cut(s) 88, 396, 1099, 1286, 1490, 2093
MbiI CCGCTC 1 cut(s) 1448
MboII GAAGA 9 cut(s) 319, 694, 1239, 1397, 1511, 1691, 2093, 2329, 2334
MfeI CAATTG 1 cut(s) 2422
MflI RGATCY 3 cut(s) 754, 1213, 1849
MhlI GDGCHC 1 cut(s) 109
MlsI TGGCCA 1 cut(s) 2130
MluNI TGGCCA 1 cut(s) 2130
Mox20I TGGCCA 1 cut(s) 2130
MscI TGGCCA 1 cut(s) 2130
MseI TTAA 9 cut(s) 189, 444, 1421, 1518, 1782, 1884, 2232, 2309, 2390
MslI CAYNNNNRTG 8 cut(s) 936, 998, 1047, 1235, 1249, 1255, 1500, 1559
Msp20I TGGCCA 1 cut(s) 2130
MspA1I CMGCKG 1 cut(s) 848
MspI CCGG 2 cut(s) 179, 411
MspR9I CCNGG 8 cut(s) 411, 1308, 1398, 1436, 1542, 1694, 1879, 2382
MunI CAATTG 1 cut(s) 2422
MvaI CCWGG 7 cut(s) 1308, 1398, 1436, 1542, 1694, 1879, 2382
MwoI GCNNNNNNNGC 8 cut(s) 136, 617, 943, 1067, 1947, 1956, 1979, 2408
NciI CCSGG 1 cut(s) 411
NdeI CATATG 2 cut(s) 94, 574
NheI GCTAGC 3 cut(s) 1505, 1856, 2240
NlaIII CATG 9 cut(s) 251, 633, 1118, 1254, 1499, 1864, 2081, 2176, 2213
NlaIV GGNNCC 8 cut(s) 21, 522, 713, 829, 929, 1836, 1972, 2272
NmuCI GTSAC 3 cut(s) 88, 1099, 2093
NspI RCATGY 2 cut(s) 633, 1118
OliI CACNNNNGTG 2 cut(s) 936, 998
PaeR7I CTCGAG 1 cut(s) 996
PagI TCATGA 1 cut(s) 247
PaqCI CACCTGC 1 cut(s) 942
PceI AGGCCT 1 cut(s) 1877
PciI ACATGT 1 cut(s) 1114
PciSI GCTCTTC 1 cut(s) 2076
PfeI GAWTC 2 cut(s) 720, 2334
PflMI CCANNNNNTGG 1 cut(s) 1934
PfoI TCCNGGA 1 cut(s) 409
PkrI GCNGC 5 cut(s) 129, 610, 1060, 1447, 2420
Ppu21I YACGTR 1 cut(s) 1917
PpuMI RGGWCCY 1 cut(s) 1835
PscI ACATGT 1 cut(s) 1114
PshBI ATTAAT 1 cut(s) 189
PsiI TTATAA 1 cut(s) 2357
Psp5II RGGWCCY 1 cut(s) 1835
Psp6I CCWGG 7 cut(s) 1306, 1396, 1434, 1540, 1692, 1877, 2380
PspEI GGTNACC 2 cut(s) 1490, 2093
PspGI CCWGG 7 cut(s) 1306, 1396, 1434, 1540, 1692, 1877, 2380
PspN4I GGNNCC 8 cut(s) 21, 522, 713, 829, 929, 1836, 1972, 2272
PspPI GGNCC 9 cut(s) 350, 521, 828, 854, 928, 1595, 1686, 1835, 1911
PspPPI RGGWCCY 1 cut(s) 1835
PspXI VCTCGAGB 1 cut(s) 996
PstI CTGCAG 1 cut(s) 613
PstNI CAGNNNCTG 1 cut(s) 617
PsuI RGATCY 3 cut(s) 754, 1213, 1849
PvuII CAGCTG 1 cut(s) 848
RseI CAYNNNNRTG 8 cut(s) 936, 998, 1047, 1235, 1249, 1255, 1500, 1559
SapI GCTCTTC 1 cut(s) 2076
SaqAI TTAA 9 cut(s) 189, 444, 1421, 1518, 1782, 1884, 2232, 2309, 2390
SatI GCNGC 5 cut(s) 128, 609, 1059, 1446, 2419
Sau96I GGNCC 9 cut(s) 350, 521, 828, 854, 928, 1595, 1686, 1835, 1911
ScaI AGTACT 1 cut(s) 269
ScrFI CCNGG 8 cut(s) 411, 1308, 1398, 1436, 1542, 1694, 1879, 2382
SduI GDGCHC 1 cut(s) 109
SexAI ACCWGGT 2 cut(s) 1396, 1692
SfaNI GCATC 7 cut(s) 508, 518, 874, 1081, 1155, 1492, 1969
SfcI CTRYAG 5 cut(s) 609, 891, 1473, 1761, 1963
Sfr274I CTCGAG 1 cut(s) 996
SinI GGWCC 5 cut(s) 828, 1595, 1686, 1835, 1911
SlaI CTCGAG 1 cut(s) 996
SmiMI CAYNNNNRTG 8 cut(s) 936, 998, 1047, 1235, 1249, 1255, 1500, 1559
SmlI CTYRAG 1 cut(s) 996
SmoI CTYRAG 1 cut(s) 996
SpeI ACTAGT 1 cut(s) 1343
SseBI AGGCCT 1 cut(s) 1877
SsiI CCGC 5 cut(s) 127, 130, 1182, 1446, 1950
SspI AATATT 1 cut(s) 1426
SspMI CTAG 8 cut(s) 156, 332, 1344, 1451, 1506, 1857, 2069, 2241
StuI AGGCCT 1 cut(s) 1877
StyD4I CCNGG 8 cut(s) 409, 1306, 1396, 1434, 1540, 1692, 1877, 2380
StyI CCWWGG 4 cut(s) 198, 715, 1185, 1717
TaiI ACGT 2 cut(s) 219, 1919
TaqI TCGA 3 cut(s) 564, 997, 1605
TatI WGTACW 2 cut(s) 267, 1582
TauI GCSGC 2 cut(s) 130, 1448
TfiI GAWTC 2 cut(s) 720, 2334
Tru1I TTAA 9 cut(s) 189, 444, 1421, 1518, 1782, 1884, 2232, 2309, 2390
Tru9I TTAA 9 cut(s) 189, 444, 1421, 1518, 1782, 1884, 2232, 2309, 2390
TscAI CASTG 6 cut(s) 91, 114, 318, 709, 968, 1758
TseFI GTSAC 3 cut(s) 88, 1099, 2093
TseI GCWGC 3 cut(s) 608, 1058, 2418
Tsp45I GTSAC 3 cut(s) 88, 1099, 2093
TspGWI ACGGA 2 cut(s) 413, 1125
TspRI CASTG 6 cut(s) 91, 114, 318, 709, 968, 1758
Van91I CCANNNNNTGG 1 cut(s) 1934
VpaK11BI GGWCC 5 cut(s) 828, 1595, 1686, 1835, 1911
VspI ATTAAT 1 cut(s) 189
XagI CCTNNNNNAGG 3 cut(s) 258, 1401, 2379
XapI RAATTY 6 cut(s) 76, 414, 664, 1015, 1623, 2168
XceI RCATGY 2 cut(s) 633, 1118
XcmI CCANNNNNNNNNTGG 2 cut(s) 2084, 2281
XhoI CTCGAG 1 cut(s) 996
XspI CTAG 8 cut(s) 156, 332, 1344, 1451, 1506, 1857, 2069, 2241
ZrmI AGTACT 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.