MD10G1335600.v1.1

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012423
Physical Location & Seq
Forward (+)
41250694 .. 41253715
3022 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1335600.v1.1.491

Sequence Viewer

Length: 678 bp
ATGGCTTTTCATTCGGAAGAGGAAAAGACCGAGGATTATCTATTCAAGATTGTTTTAATTGGTGATTCAGCCGTTGGAAAATCCAATTTGCTCGCAAGATTTGCTAGAGATGAGTTCTACCCTAATTCGAAGTCAACTATAGGAGTAGAGTTCCAAACCCAAAAGATAGATATCAATGGTAAAGAGGTCAAAGCACAGATATGGGACACAGCTGGTCAAGAGCGGTTCAGGGCTGTCACATCCGCATATTACAGAGGTGCTGTTGGTGCTCTTCTGGTATATGATATCAGTAGACGCCAGACATTTGATAGTATTGGCAGATGGCTTAACGAACTCCACACTCACTCTGACATGAATGTAGTTACGATACTTGTTGGCAACAAGTCCGATCTCAAGGATGCCAGGGAGGTACCCACTTCTGAGGGCAAAGCCTTGGCAGAAGCACAGGGTTTGTTTTTCATGGAAACATCTGCTCTAGATTCGTCCAATGTCTCTGCCGCTTTTCAGATGGTTGTGAAAGAGATCTATAACATACTAAGCCGCAAAGTTATGATATCTCAGGAGCTCAAGAAACAGGACCCCAGTTGGGTAGGGAATGGGAAGACGGTGGTTTTACAAGACGGGAGCCAAGAAGCAGCTGCAGCAGAGCCTAAAAAAGGTGGGTGCTGTTCATCTTAG

Protein Analysis

226

Amino Acids

24.86

Weight (kDa)

5.73

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 14 - 173 1e-15 ADP-ribosylation factor family
Ras PF00071 16 - 176 6e-62 Ras family
Roc PF08477 16 - 130 1e-34 Ras of Complex, Roc, domain of DAPkinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012423)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47520
fragaria_vesca FvH4_3g00730
malus_domestica MD05G1360000.v1.1 MD10G1335600.v1.1
prunus_persica Prupe.4G008200_v2.0.a1 Prupe.4G008200_v2.0.a1
pyrus_communis pycom05g32500 pycom10g28520
rosa_chinensis RchiOBHm_Chr5g0000971
rosa_laevigata RLG00000030910
rosa_multiflora Rmu_sc0000255.1_g000021
rosa_roxburghii Rroxscaffold_1G00075300
rosa_rugosa Rorug04G0388000 Rorug04G0388000
rosa_samantha Rh5AG007700 Rh5BG009800 Rh5CG008400 Rh5DG008400
rosa_wichuraiana Rw5G000800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 409
AccB1I GGYRCC 1 cut(s) 409
AccBSI CCGCTC 1 cut(s) 223
AccI GTMKAC 1 cut(s) 292
AciI CCGC 4 cut(s) 223, 243, 498, 541
AcyI GRCGYC 1 cut(s) 295
AfaI GTAC 1 cut(s) 411
AfiI CCNNNNNNNGG 2 cut(s) 586, 656
AgsI TTSAA 1 cut(s) 46
AjnI CCWGG 1 cut(s) 401
AluBI AGCT 3 cut(s) 212, 565, 638
AluI AGCT 3 cut(s) 212, 565, 638
Alw21I GWGCWC 2 cut(s) 271, 567
Alw26I GTCTC 1 cut(s) 496
ApeKI GCWGC 3 cut(s) 635, 638, 641
Asp718I GGTACC 1 cut(s) 409
AspS9I GGNCC 1 cut(s) 577
AsuHPI GGTGA 1 cut(s) 74
AsuII TTCGAA 1 cut(s) 128
AvaII GGWCC 1 cut(s) 577
BanI GGYRCC 1 cut(s) 409
BanII GRGCYC 1 cut(s) 567
BbsI GAAGAC 1 cut(s) 608
Bbv12I GWGCWC 2 cut(s) 271, 567
BbvI GCAGC 3 cut(s) 625, 647, 653
BccI CCATC 2 cut(s) 315, 502
BceAI ACGGC 1 cut(s) 56
BciT130I CCWGG 1 cut(s) 403
BcoDI GTCTC 1 cut(s) 496
BfaI CTAG 2 cut(s) 105, 476
BfmI CTRYAG 2 cut(s) 138, 639
BglII AGATCT 1 cut(s) 522
BisI GCNGC 5 cut(s) 498, 541, 636, 639, 642
BlsI GCNGC 5 cut(s) 499, 542, 637, 640, 643
Bme1390I CCNGG 1 cut(s) 403
Bme18I GGWCC 1 cut(s) 577
BmgT120I GGNCC 1 cut(s) 577
BmiI GGNNCC 3 cut(s) 411, 579, 626
BmrFI CCNGG 1 cut(s) 403
BmrI ACTGGG 1 cut(s) 576
BmsI GCATC 1 cut(s) 388
BmuI ACTGGG 1 cut(s) 576
BpiI GAAGAC 1 cut(s) 608
Bpu14I TTCGAA 1 cut(s) 128
BpuEI CTTGAG 2 cut(s) 377, 551
BsaBI GATNNNNATC 1 cut(s) 170
BsaHI GRCGYC 1 cut(s) 295
BsaJI CCNNGG 3 cut(s) 30, 402, 432
Bsc4I CCNNNNNNNGG 2 cut(s) 586, 656
Bse1I ACTGG 1 cut(s) 582
Bse8I GATNNNNATC 1 cut(s) 170
BseBI CCWGG 1 cut(s) 403
BseDI CCNNGG 3 cut(s) 30, 402, 432
BseGI GGATG 2 cut(s) 239, 403
BseJI GATNNNNATC 1 cut(s) 170
BseLI CCNNNNNNNGG 2 cut(s) 586, 656
BseMII CTCAG 2 cut(s) 411, 572
BseNI ACTGG 1 cut(s) 582
BseXI GCAGC 3 cut(s) 625, 647, 653
BshNI GGYRCC 1 cut(s) 409
BsiHKAI GWGCWC 2 cut(s) 271, 567
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 2 cut(s) 586, 656
BsmAI GTCTC 1 cut(s) 496
BsmFI GGGAC 1 cut(s) 218
Bsp119I TTCGAA 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 271, 567
Bsp143I GATC 2 cut(s) 388, 522
BspACI CCGC 4 cut(s) 223, 243, 498, 541
BspCNI CTCAG 2 cut(s) 412, 571
BspLI GGNNCC 3 cut(s) 411, 579, 626
BspMAI CTGCAG 1 cut(s) 643
BspQI GCTCTTC 1 cut(s) 276
BspT104I TTCGAA 1 cut(s) 128
BspT107I GGYRCC 1 cut(s) 409
BsrBI CCGCTC 1 cut(s) 223
BsrI ACTGG 1 cut(s) 582
BssECI CCNNGG 3 cut(s) 30, 402, 432
BssMI GATC 2 cut(s) 388, 522
BssNI GRCGYC 1 cut(s) 295
BssT1I CCWWGG 1 cut(s) 432
Bst2UI CCWGG 1 cut(s) 403
Bst4CI ACNGT 1 cut(s) 607
Bst6I CTCTTC 2 cut(s) 12, 276
BstACI GRCGYC 1 cut(s) 295
BstAPI GCANNNNNTGC 1 cut(s) 101
BstBI TTCGAA 1 cut(s) 128
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 4 cut(s) 420, 536, 558, 675
BstENI CCTNNNNNAGG 1 cut(s) 654
BstF5I GGATG 2 cut(s) 239, 403
BstKTI GATC 2 cut(s) 391, 525
BstMAI GTCTC 1 cut(s) 496
BstMBI GATC 2 cut(s) 388, 522
BstMWI GCNNNNNNNGC 3 cut(s) 101, 266, 641
BstNI CCWGG 1 cut(s) 403
BstSCI CCNGG 1 cut(s) 401
BstSFI CTRYAG 2 cut(s) 138, 639
BstV1I GCAGC 3 cut(s) 625, 647, 653
BstV2I GAAGAC 1 cut(s) 608
BstX2I RGATCY 1 cut(s) 522
BstYI RGATCY 1 cut(s) 522
BtsCI GGATG 2 cut(s) 239, 403
Cac8I GCNNGC 1 cut(s) 93
Cfr13I GGNCC 1 cut(s) 577
CseI GACGC 1 cut(s) 303
Csp6I GTAC 1 cut(s) 410
CviAII CATG 2 cut(s) 352, 460
CviQI GTAC 1 cut(s) 410
DdeI CTNAG 4 cut(s) 420, 536, 558, 675
DpnI GATC 2 cut(s) 390, 524
DpnII GATC 2 cut(s) 388, 522
Eam1104I CTCTTC 2 cut(s) 12, 276
EarI CTCTTC 2 cut(s) 12, 276
Ecl136II GAGCTC 1 cut(s) 565
Eco130I CCWWGG 1 cut(s) 432
Eco24I GRGCYC 1 cut(s) 567
Eco32I GATATC 3 cut(s) 172, 286, 555
Eco47I GGWCC 1 cut(s) 577
Eco53kI GAGCTC 1 cut(s) 565
EcoICRI GAGCTC 1 cut(s) 565
EcoNI CCTNNNNNAGG 1 cut(s) 654
EcoO109I RGGNCCY 1 cut(s) 577
EcoRII CCWGG 1 cut(s) 401
EcoRV GATATC 3 cut(s) 172, 286, 555
EcoT14I CCWWGG 1 cut(s) 432
EcoT38I GRGCYC 1 cut(s) 567
ErhI CCWWGG 1 cut(s) 432
FaeI CATG 2 cut(s) 355, 463
FaqI GGGAC 1 cut(s) 218
FatI CATG 2 cut(s) 351, 459
FblI GTMKAC 1 cut(s) 292
Fnu4HI GCNGC 5 cut(s) 498, 541, 636, 639, 642
FokI GGATG 2 cut(s) 226, 410
FriOI GRGCYC 1 cut(s) 567
Fsp4HI GCNGC 5 cut(s) 498, 541, 636, 639, 642
FspBI CTAG 2 cut(s) 105, 476
GluI GCNGC 5 cut(s) 498, 541, 636, 639, 642
HgaI GACGC 1 cut(s) 303
Hin1I GRCGYC 1 cut(s) 295
Hin1II CATG 2 cut(s) 355, 463
HincII GTYRAC 1 cut(s) 135
HindII GTYRAC 1 cut(s) 135
HinfI GANTC 2 cut(s) 65, 479
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 2 cut(s) 135, 293
Hpy188I TCNGA 5 cut(s) 16, 349, 388, 421, 507
Hpy188III TCNNGA 5 cut(s) 46, 218, 476, 560, 568
Hpy8I GTNNAC 2 cut(s) 135, 293
HpyCH4III ACNGT 1 cut(s) 607
HpyCH4V TGCA 1 cut(s) 641
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 266, 641
HpyF3I CTNAG 4 cut(s) 420, 536, 558, 675
Hsp92I GRCGYC 1 cut(s) 295
Hsp92II CATG 2 cut(s) 355, 463
KpnI GGTACC 1 cut(s) 413
Kzo9I GATC 2 cut(s) 388, 522
LguI GCTCTTC 1 cut(s) 276
LmnI GCTCC 2 cut(s) 562, 624
Lsp1109I GCAGC 3 cut(s) 625, 647, 653
LweI GCATC 1 cut(s) 388
MaeI CTAG 2 cut(s) 105, 476
MaeIII GTNAC 2 cut(s) 235, 361
MalI GATC 2 cut(s) 390, 524
MbiI CCGCTC 1 cut(s) 223
MboI GATC 2 cut(s) 388, 522
MboII GAAGA 3 cut(s) 29, 263, 613
MflI RGATCY 1 cut(s) 522
MhlI GDGCHC 2 cut(s) 271, 567
MluCI AATT 3 cut(s) 57, 85, 124
MmeI TCCRAC 1 cut(s) 55
MnlI CCTC 6 cut(s) 13, 25, 178, 248, 400, 415
MseI TTAA 2 cut(s) 56, 327
MslI CAYNNNNRTG 1 cut(s) 199
MspA1I CMGCKG 2 cut(s) 212, 638
MspR9I CCNGG 1 cut(s) 403
MvaI CCWGG 1 cut(s) 403
MwoI GCNNNNNNNGC 3 cut(s) 101, 266, 641
NdeII GATC 2 cut(s) 388, 522
NlaIII CATG 2 cut(s) 355, 463
NlaIV GGNNCC 3 cut(s) 411, 579, 626
NmuCI GTSAC 1 cut(s) 235
NspV TTCGAA 1 cut(s) 128
PciSI GCTCTTC 1 cut(s) 276
PfeI GAWTC 2 cut(s) 65, 479
PkrI GCNGC 5 cut(s) 499, 542, 637, 640, 643
PpuMI RGGWCCY 1 cut(s) 577
Psp124BI GAGCTC 1 cut(s) 567
Psp5II RGGWCCY 1 cut(s) 577
Psp6I CCWGG 1 cut(s) 401
PspGI CCWGG 1 cut(s) 401
PspN4I GGNNCC 3 cut(s) 411, 579, 626
PspPI GGNCC 1 cut(s) 577
PspPPI RGGWCCY 1 cut(s) 577
PstI CTGCAG 1 cut(s) 643
PsuI RGATCY 1 cut(s) 522
PvuII CAGCTG 2 cut(s) 212, 638
RsaI GTAC 1 cut(s) 411
RsaNI GTAC 1 cut(s) 410
RseI CAYNNNNRTG 1 cut(s) 199
SacI GAGCTC 1 cut(s) 567
SapI GCTCTTC 1 cut(s) 276
SaqAI TTAA 2 cut(s) 56, 327
SatI GCNGC 5 cut(s) 498, 541, 636, 639, 642
Sau3AI GATC 2 cut(s) 388, 522
Sau96I GGNCC 1 cut(s) 577
ScrFI CCNGG 1 cut(s) 403
SduI GDGCHC 2 cut(s) 271, 567
SetI ASST 7 cut(s) 189, 214, 259, 411, 567, 640, 661
SfaNI GCATC 1 cut(s) 388
SfcI CTRYAG 2 cut(s) 138, 639
SfuI TTCGAA 1 cut(s) 128
SinI GGWCC 1 cut(s) 577
SmiMI CAYNNNNRTG 1 cut(s) 199
SmlI CTYRAG 2 cut(s) 392, 566
SmoI CTYRAG 2 cut(s) 392, 566
Sse9I AATT 3 cut(s) 57, 85, 124
SsiI CCGC 4 cut(s) 223, 243, 498, 541
SspMI CTAG 2 cut(s) 105, 476
SstI GAGCTC 1 cut(s) 567
StyD4I CCNGG 1 cut(s) 401
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 1 cut(s) 607
TaqI TCGA 1 cut(s) 128
TaqII GACCGA 1 cut(s) 44
TasI AATT 3 cut(s) 57, 85, 124
TauI GCSGC 2 cut(s) 500, 543
TfiI GAWTC 2 cut(s) 65, 479
Tru1I TTAA 2 cut(s) 56, 327
Tru9I TTAA 2 cut(s) 56, 327
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 3 cut(s) 635, 638, 641
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 3 cut(s) 368, 448, 660
VpaK11BI GGWCC 1 cut(s) 577
XagI CCTNNNNNAGG 1 cut(s) 654
XbaI TCTAGA 1 cut(s) 475
XmiI GTMKAC 1 cut(s) 292
XspI CTAG 2 cut(s) 105, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.