Rh5AG007700

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0012423
Physical Location & Seq
Reverse (-)
528132 .. 530698
2567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG007700.1

Sequence Viewer

Length: 681 bp
ATGGCTTTTCAGTCCGAGGAAGAAAAGACTGAGGATTATCTTTTCAAGATTGTTTTAATTGGTGACTCGGCCGTTGGAAAATCTAATTTGCTGGCAAGATTTGCTAGAAATGAGTTCTTCCCTAATTCAAAGTCAACTATAGGAGTAGAGTTCCAAACCCAAAAGATTGTTATCGATGGTAAAGAAGTCAAAGCACAGATATGGGACACGGCTGGTCAAGAGCGGTTCAGGGCTGTTACATCTGCATATTACAGAGGTGCTGTTGGTGCTCTTTTGGTTTATGATATCAGTAGGCATCAGACATTTGATAGTATCGGCAGATGGCTTAATGAGCTCCACACTCACTCCGACATGAATGTTGTGACTATACTTGTCGGTAACAAGTCAGATCTTAAGGATGCCAGGGAGGTACCCACGTCTGAAGGCAAAGCCTTGGCAGAAGCACAGGGTTTGTTTTTTATGGAAACATCCGCTCTCGACTCATCCAATGTTGCTGCCGCTTTTCAGATGGTAGTGAAGGAGATCTACAGTATACTAAGTCGAAAAGTAATGATGTCCCAGGAGCTCAAGAAACAGGACCCTAGCTGGGTGGGGAGTGGCAAGACGGTGCTTTTACAGGGAGATGGGAACGAAGAAGCAGCAAGTACAGAGCCTAAAAAAGGTGGGTGCTGTTCATCTTAG

Protein Analysis

226

Amino Acids

24.89

Weight (kDa)

5.52

Isoelectric Point (pI)

37.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 14 - 172 3.7e-15 ADP-ribosylation factor family
Ras PF00071 16 - 176 1.2e-61 Ras family
Roc PF08477 16 - 130 3.8e-34 Ras of Complex, Roc, domain of DAPkinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012423)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47520
fragaria_vesca FvH4_3g00730
malus_domestica MD05G1360000.v1.1 MD10G1335600.v1.1
prunus_persica Prupe.4G008200_v2.0.a1 Prupe.4G008200_v2.0.a1
pyrus_communis pycom05g32500 pycom10g28520
rosa_chinensis RchiOBHm_Chr5g0000971
rosa_laevigata RLG00000030910
rosa_multiflora Rmu_sc0000255.1_g000021
rosa_roxburghii Rroxscaffold_1G00075300
rosa_rugosa Rorug04G0388000 Rorug04G0388000
rosa_samantha Rh5AG007700 Rh5BG009800 Rh5CG008400 Rh5DG008400
rosa_wichuraiana Rw5G000800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 409
AccB1I GGYRCC 1 cut(s) 409
AccBSI CCGCTC 2 cut(s) 223, 473
AccI GTMKAC 1 cut(s) 532
AciI CCGC 3 cut(s) 223, 471, 498
AcoI YGGCCR 1 cut(s) 69
AcuI CTGAAG 1 cut(s) 441
AfaI GTAC 2 cut(s) 411, 646
AfiI CCNNNNNNNGG 2 cut(s) 586, 659
AflII CTTAAG 1 cut(s) 392
AgsI TTSAA 2 cut(s) 46, 129
AjiI CACGTC 1 cut(s) 417
AjnI CCWGG 2 cut(s) 401, 558
AluBI AGCT 3 cut(s) 334, 565, 585
AluI AGCT 3 cut(s) 334, 565, 585
Alw21I GWGCWC 3 cut(s) 271, 336, 567
AoxI GGCC 1 cut(s) 69
ApeKI GCWGC 2 cut(s) 494, 638
Asp718I GGTACC 1 cut(s) 409
AspS9I GGNCC 1 cut(s) 577
AsuHPI GGTGA 1 cut(s) 74
AvaII GGWCC 1 cut(s) 577
BaeI ACNNNNGTAYC 2 cut(s) 401, 434
BanI GGYRCC 1 cut(s) 409
BanII GRGCYC 2 cut(s) 336, 567
BarI GAAGNNNNNNTAC 2 cut(s) 509, 541
Bbv12I GWGCWC 3 cut(s) 271, 336, 567
BbvI GCAGC 2 cut(s) 481, 650
BccI CCATC 4 cut(s) 170, 315, 502, 617
BceAI ACGGC 2 cut(s) 56, 225
BciT130I CCWGG 2 cut(s) 403, 560
BfaI CTAG 2 cut(s) 105, 582
BfmI CTRYAG 2 cut(s) 138, 526
BfrI CTTAAG 1 cut(s) 392
BglII AGATCT 2 cut(s) 388, 522
BisI GCNGC 3 cut(s) 495, 498, 639
BlsI GCNGC 3 cut(s) 496, 499, 640
Bme1390I CCNGG 2 cut(s) 403, 560
Bme18I GGWCC 1 cut(s) 577
BmgBI CACGTC 1 cut(s) 417
BmgT120I GGNCC 1 cut(s) 577
BmiI GGNNCC 2 cut(s) 411, 579
BmrFI CCNGG 2 cut(s) 403, 560
BmsI GCATC 2 cut(s) 304, 388
BpuEI CTTGAG 1 cut(s) 551
Bsa29I ATCGAT 1 cut(s) 174
BsaBI GATNNNNATC 1 cut(s) 170
BsaJI CCNNGG 4 cut(s) 15, 402, 432, 558
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bsc4I CCNNNNNNNGG 2 cut(s) 586, 659
Bse8I GATNNNNATC 1 cut(s) 170
BseBI CCWGG 2 cut(s) 403, 560
BseCI ATCGAT 1 cut(s) 174
BseDI CCNNGG 4 cut(s) 15, 402, 432, 558
BseGI GGATG 3 cut(s) 403, 467, 482
BseJI GATNNNNATC 1 cut(s) 170
BseLI CCNNNNNNNGG 2 cut(s) 586, 659
BseMII CTCAG 1 cut(s) 21
BseX3I CGGCCG 1 cut(s) 69
BseXI GCAGC 2 cut(s) 481, 650
BseYI CCCAGC 1 cut(s) 585
Bsh1285I CGRYCG 1 cut(s) 72
BshFI GGCC 1 cut(s) 71
BshNI GGYRCC 1 cut(s) 409
BshVI ATCGAT 1 cut(s) 174
BsiEI CGRYCG 1 cut(s) 72
BsiHKAI GWGCWC 3 cut(s) 271, 336, 567
BslFI GGGAC 2 cut(s) 218, 541
BslI CCNNNNNNNGG 2 cut(s) 586, 659
BsmFI GGGAC 2 cut(s) 218, 541
BsnI GGCC 1 cut(s) 71
Bsp1286I GDGCHC 3 cut(s) 271, 336, 567
Bsp143I GATC 2 cut(s) 388, 522
BspACI CCGC 3 cut(s) 223, 471, 498
BspANI GGCC 1 cut(s) 71
BspCNI CTCAG 1 cut(s) 22
BspDI ATCGAT 1 cut(s) 174
BspLI GGNNCC 2 cut(s) 411, 579
BspT107I GGYRCC 1 cut(s) 409
BspTI CTTAAG 1 cut(s) 392
BsrBI CCGCTC 2 cut(s) 223, 473
BssECI CCNNGG 4 cut(s) 15, 402, 432, 558
BssMI GATC 2 cut(s) 388, 522
BssNAI GTATAC 1 cut(s) 533
BssT1I CCWWGG 1 cut(s) 432
Bst1107I GTATAC 1 cut(s) 533
Bst2UI CCWGG 2 cut(s) 403, 560
Bst4CI ACNGT 2 cut(s) 530, 607
BstAFI CTTAAG 1 cut(s) 392
BstAPI GCANNNNNTGC 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 3 cut(s) 30, 536, 678
BstENI CCTNNNNNAGG 1 cut(s) 657
BstF5I GGATG 3 cut(s) 403, 467, 482
BstKTI GATC 2 cut(s) 391, 525
BstMBI GATC 2 cut(s) 388, 522
BstMCI CGRYCG 1 cut(s) 72
BstMWI GCNNNNNNNGC 3 cut(s) 101, 266, 331
BstNI CCWGG 2 cut(s) 403, 560
BstSCI CCNGG 2 cut(s) 401, 558
BstSFI CTRYAG 2 cut(s) 138, 526
BstV1I GCAGC 2 cut(s) 481, 650
BstX2I RGATCY 2 cut(s) 388, 522
BstYI RGATCY 2 cut(s) 388, 522
BstZ17I GTATAC 1 cut(s) 533
BstZI CGGCCG 1 cut(s) 69
Bsu15I ATCGAT 1 cut(s) 174
BsuRI GGCC 1 cut(s) 71
BsuTUI ATCGAT 1 cut(s) 174
BtrI CACGTC 1 cut(s) 417
BtsCI GGATG 3 cut(s) 403, 467, 482
Cac8I GCNNGC 1 cut(s) 93
Cfr13I GGNCC 1 cut(s) 577
ClaI ATCGAT 1 cut(s) 174
Csp6I GTAC 2 cut(s) 410, 645
CviAII CATG 1 cut(s) 352
CviQI GTAC 2 cut(s) 410, 645
DdeI CTNAG 3 cut(s) 30, 536, 678
DpnI GATC 2 cut(s) 390, 524
DpnII GATC 2 cut(s) 388, 522
EaeI YGGCCR 1 cut(s) 69
EagI CGGCCG 1 cut(s) 69
Ecl136II GAGCTC 2 cut(s) 334, 565
EclXI CGGCCG 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 432
Eco24I GRGCYC 2 cut(s) 336, 567
Eco32I GATATC 1 cut(s) 286
Eco47I GGWCC 1 cut(s) 577
Eco52I CGGCCG 1 cut(s) 69
Eco53kI GAGCTC 2 cut(s) 334, 565
Eco57I CTGAAG 1 cut(s) 441
EcoICRI GAGCTC 2 cut(s) 334, 565
EcoNI CCTNNNNNAGG 1 cut(s) 657
EcoO109I RGGNCCY 1 cut(s) 577
EcoRII CCWGG 2 cut(s) 401, 558
EcoRV GATATC 1 cut(s) 286
EcoT14I CCWWGG 1 cut(s) 432
EcoT38I GRGCYC 2 cut(s) 336, 567
ErhI CCWWGG 1 cut(s) 432
FaeI CATG 1 cut(s) 355
FaiI YATR 8 cut(s) 140, 202, 247, 282, 353, 368, 461, 533
FaqI GGGAC 2 cut(s) 218, 541
FatI CATG 1 cut(s) 351
FblI GTMKAC 1 cut(s) 532
Fnu4HI GCNGC 3 cut(s) 495, 498, 639
FokI GGATG 3 cut(s) 410, 454, 469
FriOI GRGCYC 2 cut(s) 336, 567
Fsp4HI GCNGC 3 cut(s) 495, 498, 639
FspBI CTAG 2 cut(s) 105, 582
GluI GCNGC 3 cut(s) 495, 498, 639
GsaI CCCAGC 1 cut(s) 589
HaeIII GGCC 1 cut(s) 71
Hin1II CATG 1 cut(s) 355
HincII GTYRAC 1 cut(s) 135
HindII GTYRAC 1 cut(s) 135
HinfI GANTC 2 cut(s) 65, 479
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 2 cut(s) 135, 533
Hpy188I TCNGA 6 cut(s) 16, 300, 349, 388, 421, 507
Hpy188III TCNNGA 4 cut(s) 46, 218, 476, 568
Hpy8I GTNNAC 2 cut(s) 135, 533
HpyAV CCTTC 2 cut(s) 416, 511
HpyCH4III ACNGT 2 cut(s) 530, 607
HpyCH4IV ACGT 1 cut(s) 416
HpyCH4V TGCA 1 cut(s) 245
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 266, 331
HpyF3I CTNAG 3 cut(s) 30, 536, 678
HpySE526I ACGT 1 cut(s) 416
Hsp92II CATG 1 cut(s) 355
KpnI GGTACC 1 cut(s) 413
Kzo9I GATC 2 cut(s) 388, 522
LmnI GCTCC 2 cut(s) 339, 562
Lsp1109I GCAGC 2 cut(s) 481, 650
LweI GCATC 2 cut(s) 304, 388
MaeI CTAG 2 cut(s) 105, 582
MaeII ACGT 1 cut(s) 416
MaeIII GTNAC 4 cut(s) 62, 235, 361, 377
MalI GATC 2 cut(s) 390, 524
MbiI CCGCTC 2 cut(s) 223, 473
MboI GATC 2 cut(s) 388, 522
MboII GAAGA 3 cut(s) 32, 109, 644
MflI RGATCY 2 cut(s) 388, 522
MhlI GDGCHC 3 cut(s) 271, 336, 567
MluCI AATT 3 cut(s) 57, 85, 124
MlyI GAGTC 2 cut(s) 59, 473
MmeI TCCRAC 2 cut(s) 55, 372
MnlI CCTC 4 cut(s) 10, 25, 248, 400
MseI TTAA 3 cut(s) 56, 327, 393
MslI CAYNNNNRTG 1 cut(s) 199
MspCI CTTAAG 1 cut(s) 392
MspR9I CCNGG 2 cut(s) 403, 560
MvaI CCWGG 2 cut(s) 403, 560
MwoI GCNNNNNNNGC 3 cut(s) 101, 266, 331
NdeII GATC 2 cut(s) 388, 522
NlaIII CATG 1 cut(s) 355
NlaIV GGNNCC 2 cut(s) 411, 579
NmeAIII GCCGAG 1 cut(s) 47
NmuCI GTSAC 2 cut(s) 62, 361
PkrI GCNGC 3 cut(s) 496, 499, 640
PleI GAGTC 2 cut(s) 59, 473
PpsI GAGTC 2 cut(s) 59, 473
PpuMI RGGWCCY 1 cut(s) 577
Psp124BI GAGCTC 2 cut(s) 336, 567
Psp5II RGGWCCY 1 cut(s) 577
Psp6I CCWGG 2 cut(s) 401, 558
PspFI CCCAGC 1 cut(s) 585
PspGI CCWGG 2 cut(s) 401, 558
PspN4I GGNNCC 2 cut(s) 411, 579
PspPI GGNCC 1 cut(s) 577
PspPPI RGGWCCY 1 cut(s) 577
PsuI RGATCY 2 cut(s) 388, 522
RsaI GTAC 2 cut(s) 411, 646
RsaNI GTAC 2 cut(s) 410, 645
RseI CAYNNNNRTG 1 cut(s) 199
SacI GAGCTC 2 cut(s) 336, 567
SaqAI TTAA 3 cut(s) 56, 327, 393
SatI GCNGC 3 cut(s) 495, 498, 639
Sau3AI GATC 2 cut(s) 388, 522
Sau96I GGNCC 1 cut(s) 577
SchI GAGTC 2 cut(s) 59, 473
ScrFI CCNGG 2 cut(s) 403, 560
SduI GDGCHC 3 cut(s) 271, 336, 567
SetI ASST 7 cut(s) 259, 336, 411, 419, 567, 587, 664
SfaNI GCATC 2 cut(s) 304, 388
SfcI CTRYAG 2 cut(s) 138, 526
SinI GGWCC 1 cut(s) 577
SmiMI CAYNNNNRTG 1 cut(s) 199
SmlI CTYRAG 2 cut(s) 392, 566
SmoI CTYRAG 2 cut(s) 392, 566
Sse9I AATT 3 cut(s) 57, 85, 124
SsiI CCGC 3 cut(s) 223, 471, 498
SspMI CTAG 2 cut(s) 105, 582
SstI GAGCTC 2 cut(s) 336, 567
StyD4I CCNGG 2 cut(s) 401, 558
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 2 cut(s) 530, 607
TaiI ACGT 1 cut(s) 419
TaqI TCGA 3 cut(s) 174, 477, 541
TasI AATT 3 cut(s) 57, 85, 124
TatI WGTACW 1 cut(s) 644
TauI GCSGC 1 cut(s) 500
Tru1I TTAA 3 cut(s) 56, 327, 393
Tru9I TTAA 3 cut(s) 56, 327, 393
TseFI GTSAC 2 cut(s) 62, 361
TseI GCWGC 2 cut(s) 494, 638
Tsp45I GTSAC 2 cut(s) 62, 361
TspDTI ATGAA 2 cut(s) 368, 663
Vha464I CTTAAG 1 cut(s) 392
VpaK11BI GGWCC 1 cut(s) 577
XagI CCTNNNNNAGG 1 cut(s) 657
XmiI GTMKAC 1 cut(s) 532
XspI CTAG 2 cut(s) 105, 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.