RLG00000030910

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012423
Physical Location & Seq
Reverse (-)
556885 .. 559242
2358 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030910

Sequence Viewer

Length: 831 bp
ATGTGTGCTTCTGACGACCCTCTCTGCTTTTATCCTCCCACCTCTCTCCGCTGGCCTCCTATCTCCCTTCTCCGCCGATCTCCGTCGTTGACTCCCGTCGATCACAACCCTCCACGGTTTGGACGCGCCCGAGTTATTGATCCGTTATCCATATTCAATCTGTCCGAGGAAGAAAAGACTGAGGATTATCTTTTCAAGATTGTTTTAATTGGTGACTCGGCGGTTGGAAAATCTAATTTGCTGGCAAGATTTGCTAGAAATGAGTTCTTCCCTAATTCAAAGTCAACTATAGGAGTAGAGTTCCAAACCCAAAAGATCGTTATCGATGGTAAAGAAGTCAAAGCACAGATATGGGACACGGCTGGTCAAGAGCGGTTCAGGGCTGTTACATCTGCATATTACAGAGGTGCTGTTGGTGCTCTTTTGGTTTATGATATCAGTAGGCATCAGACATTTGATAGTATCGGCAGATGGCTTAATGAGCTCCACACTCACTCCGACATGAATGTTGTGACGATACTTGTCGGTAACAAGTCAGATCTTAAGGATGCCAGGGAGGTACCGACGTCTGAAGGCAAAGCCTTGGCAGAAGCACAGGGTTTGTTTTTTATGGAAACATCTGCTCTCGACTCATCCAATGTTGCTGCCGCCTTTCAGACGGTAGTGAAGGAGATCTACAGTATACTAAGTCGAAAAGTAATGATGTCCCAGGAGCTCAAGAAACAGGACCCTAGCTGGGTGGGGAGTGGCAAGACGGTGCTTTTACAGGGAGATGGGAACGAAGAAGCAGCAAGTACAGAGCCTAAAAAAGGTGGGTGCTGTTCATCTTAG

Protein Analysis

277

Amino Acids

30.5

Weight (kDa)

6.14

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 63 - 221 6.2e-15 ADP-ribosylation factor family
Ras PF00071 66 - 226 1.4e-61 Ras family
Roc PF08477 66 - 180 6.3e-34 Ras of Complex, Roc, domain of DAPkinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012423)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47520
fragaria_vesca FvH4_3g00730
malus_domestica MD05G1360000.v1.1 MD10G1335600.v1.1
prunus_persica Prupe.4G008200_v2.0.a1 Prupe.4G008200_v2.0.a1
pyrus_communis pycom05g32500 pycom10g28520
rosa_chinensis RchiOBHm_Chr5g0000971
rosa_laevigata RLG00000030910
rosa_multiflora Rmu_sc0000255.1_g000021
rosa_roxburghii Rroxscaffold_1G00075300
rosa_rugosa Rorug04G0388000 Rorug04G0388000
rosa_samantha Rh5AG007700 Rh5BG009800 Rh5CG008400 Rh5DG008400
rosa_wichuraiana Rw5G000800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 569
Acc65I GGTACC 1 cut(s) 559
AccB1I GGYRCC 1 cut(s) 559
AccB7I CCANNNNNTGG 1 cut(s) 119
AccBSI CCGCTC 1 cut(s) 373
AccI GTMKAC 1 cut(s) 682
AccII CGCG 1 cut(s) 126
AciI CCGC 5 cut(s) 49, 73, 221, 373, 648
AclWI GGATC 1 cut(s) 134
AcuI CTGAAG 1 cut(s) 591
AcyI GRCGYC 1 cut(s) 566
AfaI GTAC 2 cut(s) 561, 796
AfiI CCNNNNNNNGG 3 cut(s) 119, 736, 809
AflII CTTAAG 1 cut(s) 542
AgsI TTSAA 3 cut(s) 157, 196, 279
AjnI CCWGG 2 cut(s) 551, 708
AluBI AGCT 3 cut(s) 484, 715, 735
AluI AGCT 3 cut(s) 484, 715, 735
Alw21I GWGCWC 3 cut(s) 421, 486, 717
AlwI GGATC 1 cut(s) 134
Ama87I CYCGRG 1 cut(s) 129
AoxI GGCC 1 cut(s) 53
ApeKI GCWGC 2 cut(s) 644, 788
Asp718I GGTACC 1 cut(s) 559
AspLEI GCGC 1 cut(s) 128
AspS9I GGNCC 1 cut(s) 727
AsuHPI GGTGA 1 cut(s) 224
AvaI CYCGRG 1 cut(s) 129
AvaII GGWCC 1 cut(s) 727
BaeI ACNNNNGTAYC 2 cut(s) 551, 584
BanI GGYRCC 1 cut(s) 559
BanII GRGCYC 2 cut(s) 486, 717
BarI GAAGNNNNNNTAC 2 cut(s) 659, 691
Bbv12I GWGCWC 3 cut(s) 421, 486, 717
BbvI GCAGC 2 cut(s) 631, 800
BccI CCATC 3 cut(s) 320, 465, 767
BceAI ACGGC 1 cut(s) 375
BciT130I CCWGG 2 cut(s) 553, 710
BfaI CTAG 2 cut(s) 255, 732
BfmI CTRYAG 2 cut(s) 288, 676
BfrI CTTAAG 1 cut(s) 542
BglII AGATCT 2 cut(s) 538, 672
BisI GCNGC 3 cut(s) 645, 648, 789
BlsI GCNGC 3 cut(s) 646, 649, 790
Bme1390I CCNGG 2 cut(s) 553, 710
Bme18I GGWCC 1 cut(s) 727
BmeT110I CYCGRG 1 cut(s) 129
BmgT120I GGNCC 1 cut(s) 727
BmiI GGNNCC 2 cut(s) 561, 729
BmrFI CCNGG 2 cut(s) 553, 710
BmsI GCATC 2 cut(s) 454, 538
BoxI GACNNNNGTC 1 cut(s) 95
BpuEI CTTGAG 1 cut(s) 701
Bsa29I ATCGAT 1 cut(s) 324
BsaBI GATNNNNATC 1 cut(s) 320
BsaHI GRCGYC 1 cut(s) 566
BsaJI CCNNGG 5 cut(s) 113, 165, 552, 582, 708
BsaXI ACNNNNNCTCC 2 cut(s) 479, 509
Bsc4I CCNNNNNNNGG 3 cut(s) 119, 736, 809
Bse8I GATNNNNATC 1 cut(s) 320
BseBI CCWGG 2 cut(s) 553, 710
BseCI ATCGAT 1 cut(s) 324
BseDI CCNNGG 5 cut(s) 113, 165, 552, 582, 708
BseGI GGATG 2 cut(s) 553, 632
BseJI GATNNNNATC 1 cut(s) 320
BseLI CCNNNNNNNGG 3 cut(s) 119, 736, 809
BseMII CTCAG 1 cut(s) 171
BseXI GCAGC 2 cut(s) 631, 800
BseYI CCCAGC 1 cut(s) 735
Bsh1236I CGCG 1 cut(s) 126
BshFI GGCC 1 cut(s) 55
BshNI GGYRCC 1 cut(s) 559
BshVI ATCGAT 1 cut(s) 324
BsiHKAI GWGCWC 3 cut(s) 421, 486, 717
BsiHKCI CYCGRG 1 cut(s) 129
BslFI GGGAC 2 cut(s) 368, 691
BslI CCNNNNNNNGG 3 cut(s) 119, 736, 809
BsmFI GGGAC 2 cut(s) 368, 691
BsnI GGCC 1 cut(s) 55
BsoBI CYCGRG 1 cut(s) 129
Bsp1286I GDGCHC 3 cut(s) 421, 486, 717
Bsp143I GATC 6 cut(s) 77, 100, 139, 315, 538, 672
BspACI CCGC 5 cut(s) 49, 73, 221, 373, 648
BspANI GGCC 1 cut(s) 55
BspCNI CTCAG 1 cut(s) 172
BspDI ATCGAT 1 cut(s) 324
BspFNI CGCG 1 cut(s) 126
BspLI GGNNCC 2 cut(s) 561, 729
BspPI GGATC 1 cut(s) 134
BspT107I GGYRCC 1 cut(s) 559
BspTI CTTAAG 1 cut(s) 542
BsrBI CCGCTC 1 cut(s) 373
BssECI CCNNGG 5 cut(s) 113, 165, 552, 582, 708
BssMI GATC 6 cut(s) 77, 100, 139, 315, 538, 672
BssNAI GTATAC 1 cut(s) 683
BssNI GRCGYC 1 cut(s) 566
BssT1I CCWWGG 1 cut(s) 582
Bst1107I GTATAC 1 cut(s) 683
Bst2UI CCWGG 2 cut(s) 553, 710
Bst4CI ACNGT 4 cut(s) 117, 661, 680, 757
BstACI GRCGYC 1 cut(s) 566
BstAFI CTTAAG 1 cut(s) 542
BstAPI GCANNNNNTGC 1 cut(s) 251
BstC8I GCNNGC 2 cut(s) 53, 243
BstDEI CTNAG 3 cut(s) 180, 686, 828
BstDSI CCRYGG 1 cut(s) 113
BstENI CCTNNNNNAGG 1 cut(s) 807
BstF5I GGATG 2 cut(s) 553, 632
BstFNI CGCG 1 cut(s) 126
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 6 cut(s) 80, 103, 142, 318, 541, 675
BstMBI GATC 6 cut(s) 77, 100, 139, 315, 538, 672
BstMWI GCNNNNNNNGC 3 cut(s) 251, 416, 481
BstNI CCWGG 2 cut(s) 553, 710
BstPAI GACNNNNGTC 1 cut(s) 95
BstSCI CCNGG 2 cut(s) 551, 708
BstSFI CTRYAG 2 cut(s) 288, 676
BstUI CGCG 1 cut(s) 126
BstV1I GCAGC 2 cut(s) 631, 800
BstX2I RGATCY 2 cut(s) 538, 672
BstYI RGATCY 2 cut(s) 538, 672
BstZ17I GTATAC 1 cut(s) 683
Bsu15I ATCGAT 1 cut(s) 324
BsuRI GGCC 1 cut(s) 55
BsuTUI ATCGAT 1 cut(s) 324
BtgI CCRYGG 1 cut(s) 113
BtsCI GGATG 2 cut(s) 553, 632
Cac8I GCNNGC 2 cut(s) 53, 243
CfoI GCGC 1 cut(s) 128
Cfr13I GGNCC 1 cut(s) 727
ClaI ATCGAT 1 cut(s) 324
CseI GACGC 1 cut(s) 132
Csp6I GTAC 2 cut(s) 560, 795
CviAII CATG 1 cut(s) 502
CviJI RGCY 9 cut(s) 55, 362, 383, 475, 484, 581, 715, 735, 802
CviKI_1 RGCY 9 cut(s) 55, 362, 383, 475, 484, 581, 715, 735, 802
CviQI GTAC 2 cut(s) 560, 795
DdeI CTNAG 3 cut(s) 180, 686, 828
DpnI GATC 6 cut(s) 79, 102, 141, 317, 540, 674
DpnII GATC 6 cut(s) 77, 100, 139, 315, 538, 672
EciI GGCGGA 1 cut(s) 62
Ecl136II GAGCTC 2 cut(s) 484, 715
Eco130I CCWWGG 1 cut(s) 582
Eco24I GRGCYC 2 cut(s) 486, 717
Eco32I GATATC 1 cut(s) 436
Eco47I GGWCC 1 cut(s) 727
Eco53kI GAGCTC 2 cut(s) 484, 715
Eco57I CTGAAG 1 cut(s) 591
Eco88I CYCGRG 1 cut(s) 129
EcoICRI GAGCTC 2 cut(s) 484, 715
EcoNI CCTNNNNNAGG 1 cut(s) 807
EcoO109I RGGNCCY 1 cut(s) 727
EcoRII CCWGG 2 cut(s) 551, 708
EcoRV GATATC 1 cut(s) 436
EcoT14I CCWWGG 1 cut(s) 582
EcoT38I GRGCYC 2 cut(s) 486, 717
ErhI CCWWGG 1 cut(s) 582
FaeI CATG 1 cut(s) 505
FaiI YATR 8 cut(s) 152, 290, 352, 397, 432, 503, 611, 683
FaqI GGGAC 2 cut(s) 368, 691
FatI CATG 1 cut(s) 501
FblI GTMKAC 1 cut(s) 682
Fnu4HI GCNGC 3 cut(s) 645, 648, 789
FokI GGATG 2 cut(s) 560, 619
FriOI GRGCYC 2 cut(s) 486, 717
Fsp4HI GCNGC 3 cut(s) 645, 648, 789
FspBI CTAG 2 cut(s) 255, 732
GlaI GCGC 1 cut(s) 127
GluI GCNGC 3 cut(s) 645, 648, 789
GsaI CCCAGC 1 cut(s) 739
HaeIII GGCC 1 cut(s) 55
HgaI GACGC 1 cut(s) 132
HhaI GCGC 1 cut(s) 128
Hin1I GRCGYC 1 cut(s) 566
Hin1II CATG 1 cut(s) 505
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HincII GTYRAC 2 cut(s) 90, 285
HindII GTYRAC 2 cut(s) 90, 285
HinfI GANTC 3 cut(s) 91, 215, 629
HphI GGTGA 1 cut(s) 224
Hpy166II GTNNAC 3 cut(s) 90, 285, 683
Hpy188I TCNGA 7 cut(s) 13, 166, 450, 499, 538, 571, 657
Hpy188III TCNNGA 4 cut(s) 196, 368, 626, 718
Hpy8I GTNNAC 3 cut(s) 90, 285, 683
Hpy99I CGWCG 3 cut(s) 88, 101, 568
HpyAV CCTTC 3 cut(s) 77, 566, 661
HpyCH4III ACNGT 4 cut(s) 117, 661, 680, 757
HpyCH4IV ACGT 1 cut(s) 566
HpyCH4V TGCA 1 cut(s) 395
HpyF10VI GCNNNNNNNGC 3 cut(s) 251, 416, 481
HpyF3I CTNAG 3 cut(s) 180, 686, 828
HpySE526I ACGT 1 cut(s) 566
Hsp92I GRCGYC 1 cut(s) 566
Hsp92II CATG 1 cut(s) 505
HspAI GCGC 1 cut(s) 126
KpnI GGTACC 1 cut(s) 563
Kzo9I GATC 6 cut(s) 77, 100, 139, 315, 538, 672
LmnI GCTCC 2 cut(s) 489, 712
Lsp1109I GCAGC 2 cut(s) 631, 800
LweI GCATC 2 cut(s) 454, 538
MaeI CTAG 2 cut(s) 255, 732
MaeII ACGT 1 cut(s) 566
MaeIII GTNAC 4 cut(s) 212, 385, 511, 527
MalI GATC 6 cut(s) 79, 102, 141, 317, 540, 674
MbiI CCGCTC 1 cut(s) 373
MboI GATC 6 cut(s) 77, 100, 139, 315, 538, 672
MboII GAAGA 3 cut(s) 182, 259, 794
MflI RGATCY 2 cut(s) 538, 672
MhlI GDGCHC 3 cut(s) 421, 486, 717
MluCI AATT 3 cut(s) 207, 235, 274
MlyI GAGTC 3 cut(s) 85, 209, 623
MmeI TCCRAC 2 cut(s) 205, 522
MnlI CCTC 9 cut(s) 30, 45, 52, 66, 120, 160, 175, 398, 550
MseI TTAA 3 cut(s) 206, 477, 543
MslI CAYNNNNRTG 1 cut(s) 349
MspA1I CMGCKG 1 cut(s) 51
MspCI CTTAAG 1 cut(s) 542
MspR9I CCNGG 2 cut(s) 553, 710
MvaI CCWGG 2 cut(s) 553, 710
MvnI CGCG 1 cut(s) 126
MwoI GCNNNNNNNGC 3 cut(s) 251, 416, 481
NdeII GATC 6 cut(s) 77, 100, 139, 315, 538, 672
NlaIII CATG 1 cut(s) 505
NlaIV GGNNCC 2 cut(s) 561, 729
NmeAIII GCCGAG 1 cut(s) 197
NmuCI GTSAC 2 cut(s) 212, 511
PflMI CCANNNNNTGG 1 cut(s) 119
PkrI GCNGC 3 cut(s) 646, 649, 790
PleI GAGTC 3 cut(s) 85, 209, 623
PpsI GAGTC 3 cut(s) 85, 209, 623
PpuMI RGGWCCY 1 cut(s) 727
PshAI GACNNNNGTC 1 cut(s) 95
Psp124BI GAGCTC 2 cut(s) 486, 717
Psp5II RGGWCCY 1 cut(s) 727
Psp6I CCWGG 2 cut(s) 551, 708
PspFI CCCAGC 1 cut(s) 735
PspGI CCWGG 2 cut(s) 551, 708
PspN4I GGNNCC 2 cut(s) 561, 729
PspPI GGNCC 1 cut(s) 727
PspPPI RGGWCCY 1 cut(s) 727
PsuI RGATCY 2 cut(s) 538, 672
RsaI GTAC 2 cut(s) 561, 796
RsaNI GTAC 2 cut(s) 560, 795
RseI CAYNNNNRTG 1 cut(s) 349
SacI GAGCTC 2 cut(s) 486, 717
SaqAI TTAA 3 cut(s) 206, 477, 543
SatI GCNGC 3 cut(s) 645, 648, 789
Sau3AI GATC 6 cut(s) 77, 100, 139, 315, 538, 672
Sau96I GGNCC 1 cut(s) 727
SchI GAGTC 3 cut(s) 85, 209, 623
ScrFI CCNGG 2 cut(s) 553, 710
SduI GDGCHC 3 cut(s) 421, 486, 717
SetI ASST 8 cut(s) 44, 409, 486, 561, 569, 717, 737, 814
SfaNI GCATC 2 cut(s) 454, 538
SfcI CTRYAG 2 cut(s) 288, 676
SinI GGWCC 1 cut(s) 727
SmiMI CAYNNNNRTG 1 cut(s) 349
SmlI CTYRAG 2 cut(s) 542, 716
SmoI CTYRAG 2 cut(s) 542, 716
Sse9I AATT 3 cut(s) 207, 235, 274
SsiI CCGC 5 cut(s) 49, 73, 221, 373, 648
SspMI CTAG 2 cut(s) 255, 732
SstI GAGCTC 2 cut(s) 486, 717
StyD4I CCNGG 2 cut(s) 551, 708
StyI CCWWGG 1 cut(s) 582
TaaI ACNGT 4 cut(s) 117, 661, 680, 757
TaiI ACGT 1 cut(s) 569
TaqI TCGA 4 cut(s) 99, 324, 627, 691
TasI AATT 3 cut(s) 207, 235, 274
TatI WGTACW 1 cut(s) 794
TauI GCSGC 1 cut(s) 650
Tru1I TTAA 3 cut(s) 206, 477, 543
Tru9I TTAA 3 cut(s) 206, 477, 543
TseFI GTSAC 2 cut(s) 212, 511
TseI GCWGC 2 cut(s) 644, 788
Tsp45I GTSAC 2 cut(s) 212, 511
TspDTI ATGAA 2 cut(s) 518, 813
TspGWI ACGGA 2 cut(s) 72, 132
Van91I CCANNNNNTGG 1 cut(s) 119
Vha464I CTTAAG 1 cut(s) 542
VpaK11BI GGWCC 1 cut(s) 727
XagI CCTNNNNNAGG 1 cut(s) 807
XmiI GTMKAC 1 cut(s) 682
XspI CTAG 2 cut(s) 255, 732
ZraI GACGTC 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.