Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0011696

Physical Location & Seq

Genomic Coordinates Reverse (-)

1265333 .. 1286042

Gene Structure 20710 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1016100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6030 bp

ATGGATTGGGTCACGCGTCCACGGGAGTTTTCTTCTCCGCGTCAATTCGAGAACCGGCCGCGACCAGTCACCAAACCCAAAACCCATACTCTCTCACCGGAAGTACCCGGTCAAACCGGTTACCCATTAGATCCATTTTTCTTCCTCCTTTATTACCTTTTGCCCTTTCCCCTTTCCCTTTTCAATTTTCAAAACCCACATTACCTCCCACCACCTTCAAACCACGTCGTCCGAGCTGGAGACCGATTCAACTCGGCATCGACTCGGCCTTCTAGGGTTTCCTATGCACTGATTGATTTCCCAAATCTGAAAATGTCTCGAGTTGAACAGCGATGGGAGCATCTAGTCCGTGCCGTATTGAGCAGGGCGAGGATGGGGGCTGATGCGTATGGCCGGCATGCTACTGGAATTGCTGGAAATGTTCCTTCCTCACTTGCAAACAATAGGGATATTGATGAAATTCTGAGAGCTGCTGATGAAATCCAAGACGAAGATCCCACTATTTCCAGAATCTTATGCGAGCATGCTTATTCTCTGGCTCAGAATTTGGATCCCAATAGTGAAGGAAGGGGAGTATTACAGTTCAAGACTGGATTGATGTCTGTCATTAAGCAAAAGCTTGCAAAGAAGGAAGGTGGAACCATAGATAGAAGCCAGGACATTGCTCGCTTGCAAGAGTTCTACAAACTGTACAGACAGAAGAATAATGTAGAAAAGCTGCGGGAGGAAGAAATGAAGTTGAGGGAGTCTAGTGCTTTCAGTGGGAACCTGGGCGAGTTAGAGAGAAAAACAGTGAAGCGGAAAAAGGTTTTTGCAACTCTAAGAGTTTTAGGGATAGTTTTGGAGCAGCTGACTGAGGAAATTCCTGAAGAGTTGAAACGGGTAATGGAATCAGATGCGGCAATGACCGAGGACCTGATTGCCTACAATATTATTCCTCTGGATGCTCCCAACATTACAAATTCTGTTGTCTCTTTGCCTGAGGTACAAGCAGCAGTGTCAGCGTTAAAGTACTTCAGTAACCTACCAAAATTGCCATCAGATTTTCCAATCCCTGCTACAAGAGATCCAGACATGTTTGATTTTCTGTATTATACTTTTGGATTTCAGAAAGATAATGTCTCTAACCAGCGCGAACATATTGTTCACCTTCTCGCAAATGAGCAATCTCGGCTTCGTATTCCTGAAGTAATTGAAGCTAAATTGGACGAGGCTGCAGCACAAAGTGTATTTCTGAAGTCCCTTGAGAACTATATTAAATGGTGTGACTACTTATGCATTCAGCCAGTTTGGAGCAATTTGGACGCAGTTACCAAGGAAAAAAAATTGCTGTTTGTTTCTCTGTACTTTCTGATCTGGGGTGAATCAGGCAATATTAGATTCCTTCCTGAATGCTTGTGTTACATATTTCATCATATGGCAAGGGAAATGGATGAAATTCTAAGGCAGCAGATTGCACAGCCAGCAAACAGTTGTAATTCTGAAAATGGAGTTTCTTTTCTTGACCAAGTTATCTATCCTCTCTATGAGGTTGTGGCTGCGGAAGCTGCAAACAATGATAATGGAAAAGCACCACATTCTGCCTGGCGCAATTATGATGATTTCAACGAGTACTTCTGGTCACTTCATTGCTTTGAACTTAGTTGGCCTTGGCGCAGAGGGTCATCCTTTTTTCAAAAGCCAGTACGAAGGTCAAAGAATATACTTAAATCTGGCAGAAGTCAACATCGAGGAAAAACTTCATTTGTTGAGCACAGGACATTTCTCCATCTTTACCATAGTTTCCACCGTCTTTGGATATTCCTTGTGATGATGTTTCAGGGACTGACTATAATTGCATTCAATAATGGAAAGCTTGATTCGAAGTGTATTCGGGAAGTTCTCAGCCTTGGTCCAACATTTGTTGCAATGAAATTTCTTGAGAGTGTTCTTGACATACGCATGATGTATGGTGCATATTCTACAACACGACGCCTAGCAGTTTCTCGAATTTTTCTCCGATTTGTTTGGTTTAGCACTACATCAGTGGTCATAACTTTTCTTTATGTGAAAGCTCTCCAGGAAGAGAGTAAACAAAATGGAAACTCAATTATTTATAGACTGTATGTGATCGTTATAGGCACATATGCTGGTATCCAGTTCTTCATTAGCTTTTTTATGAGGATACCAGCCTGCCACAATCTGACTAATCAATGCGATCGCTTTCCTCTGATTAGTTTTTTCAAGTGGATGCGCCAGGAACGGCATTATGTTGGACGTGGGATGTATGAGAGGACCACTGATTTTATCAAGTATATGCTTTTCTGGCTTGTGATTTTGAGTGGAAAATTTTCTTTTGCTTATTTTCTTCAGATCAAGCCATTAGTGAAACCAACTAAGACAATAGTGACAATGGATTCTATACTCTATTCTTGGCATGACTTAGTTTCTAAAAACAATCATAATGCCTTGACAGTTGCTAGTTTATGGGCTCCTGTGATACTTATTTATCTTTTAGATCTCCATGTATTTTACACCATTATATCTGGTGTGTGGGGATTCTTGCTTGGAGCAAGAGATCGACTTGGAGAGATTAGATCATTGGAAGCACTTCATCAACTTTTTGAGCAGTTTCCTGGAGCTTTTATGAACACTCTTCATGTGTCTCTTCCTGACAGGACTTCCCAACAGCCTTCCAGTGAGGACCTTGAGAAGAACAAGGTTGATGCTGGTAGATTCTCTCCGTTTTGGAATGAGATTATAAGAAATTTGAGGGAAGAAGATTATATCACCAACTTAGAAATGGAGTTGCTTGCCATGCCTAAAAACTCTGGAAATCTTCCCTTGGTTCAGTGGCCGCTTTTTCTTCTCTCTAGCAAGATATTTGTGGCCAAGGATATTGCTGTTGAGAGCAGAGATTCACAAGATGAACTTTGGGAGAGAATTTCAAGAGATGACTATATGAAGTATGCTGTCCAGGAGTGCTTCCACAGCCTTAAGCTAATCTTGGATGAAATATTGGAAGGCGAAGGAAAGATGTGGGTTGAACAGGTGTACAAGGATATTCATTCAAGCATAGAGAAGAAGAGTATTCATGTTGATTATCAACTGAATAAGCTGCCACTTGTGATATCAAGAGTTACTGCATTAATGGGAATTCTGAAAGAAGGTGGAACACCTGAACTGGAGAAAGGTGCAGTTAAGGCTGTTCAAGATCTTTATGATGTTGTGCACCATGACATCCTTTCTATGGACCTGAGGGGAAACTATGAGACATGGAAATTGTTGTCAAATGCAAGGACAGAAGGTCGTCTATTTGCAAAGTTGAAGTGGCCTAAGGACCCTGAGTTGAGATCGCAAGTCAAAAGATTGTATTCGCTGTTAACCATTAAAGATTCGGCTGCCAATGTACCCAATAATCTGGAGGCAAGACGCAGGCTCGAGTTCTTCACAAATTCCCTTTTTATGGAGATGCCTGCAGCAAAGCCAGTCCGTGAGATGTTATCGTTCAGTGTGTTTACTCCATACTATGCTGAAATTGTGCTGTATAGTATGGCTGAGCTCCAGAAGAAAAATGAGGATGGAATATCAATATTATTTTATCTTCAAAAAATTTATCCAGATGAGTGGAAGAACTTTCTTGCCCGAATTGGCCGTGACGAAAATACTCTTGACTCTGAACTCTCTGAGAATCCCACTGATATCCTTGAGCTTCGTTTTTGGGCCTCTTACCGAGGTCAAACATTAGCCAGAACAGTTCGTGGAATGATGTATTATAGGAAAGCTCTTATGCTTCAGACCTATTTGGAGAGAATAAATTCTGGAGGATCAGATGTGGAGGGTGCAATTTCCAGTAATGACGCAACTGATACTCGAGCGTTTGAGTTATCTCCTGAAGCACGTGCTCATGCTGACTTAAAGTTTACATATGTTGTAACGTGTCAAATATATGGAAAACAGAAAGAAGGCCAAAAACCTGAGGCTGCAGATATAGCAATGCTTATGCAAAGATATGAGGCTCTTCGGGTTGCTTTTATTGATGAAGTTGAGACTTTGAAGGATGGTAAAGTGCACAGAGAGTTCTACTCAAAACTCGTAAAGGCTGATATCAATGGGAAGGACAAGGAAATCTACTCCATAAAACTGCCTGGAAATCCGAAACTTGGTGAAGGAAAACCGGAGAATCAAAACCATGCAATTGTATTTACTCGTGGAAATGCAATTCAGACAATTGACATGAATCAGGATAACTATTTTGAGGAAGCTTTGAAGATGAGAAACCTACTTGAAGAATTCCATACTGATCATGGTATCCGACGTCCTACAATTCTTGGCGTCAGAGAGCATGTCTTTACAGGAAGTGTTTCTTCTTTAGCATCATTCATGTCCAATCAGGAAGCAAGCTTTGTAACTCTAGCTCAGCGTGTTCTTGCAAATCCTTTGAAGGTCCGTATGCATTATGGTCACCCTGATGTGTTTGATAGAGTGTTCCACCTAACTCGAGGTGGTATTAGCAAGGCTTCTCGTGTAATTAACATTAGTGAAGATATCTTTGCAGGATTCAACTCAACTTTACGCCAAGGAAATGTGACTCACCACGAGTACATTCAGGTTGGCAAGGGACGAGATGTTGGGCTCAATCAAATTGCTGTATTTGAGGGGAAGGTTTCTGGTGGTAACGGTGAACAAGTTCTAAGTCGAGACGTATACAGGCTTGGGCAGCAATTTGATTTTTTTCGGATGCTGTCATTTTTTTATACCACCGTTGGCTACTATTTTTGTACAATGTTAACAGTGCTGATGGTATTTATTTTTCTCTATGGAAAAATATATTTGGCATTATCTGGTGTTGAGTCAAGGTTGACAGACATAGCTTTGGTGACAAAGAATACAGCGTTGACTGCTGCTCTTAATACCCAATTTCTCATCCAAATTGGTATCTTCACTGCAGTCCCAATGGTTCTAGGCTTCATCTTGGAACAAGGTTTCCTGAGGGCCATGGTCAGTTTTCTCACAATGCAGCTGCAGCTTTGTTCTGTCTACTTCACATTCTCTTTGGGTACAAAAACTCATTATTTTGGCCGGACTATTCTTCATGGTGGTGCAAAGTATCAAGCAACAGGCAGGGGATTTGTAGTTCGTCACATTAAATTTTCAGAGAACTACAGGCTCTACTCTCGCAGTCATTTTGTTAAAGGATTGGAAGTAGTGCTTTTGTTGGTCGTGTACCTTGCCTACGGCGATAATGATGGGAGTGCTCTAACATATATTTTTCTCACAGTGACCAGTTGGTTTATGGCACTTTCTTGGCTCTTTGCTCCATATTTGTTCAATCCGTCTGGTTTTGAGTGGCAGAAGATTGTGGAGGATTTTCGAGATTGGACAAATTGGCTTCTTTACAGAGGTGGAATTGGAGTTAAGGGTGAAGAAAGCTGGGAAGCTTGGTGGGAGGAAGAACTGGCACATATTCGGACTTTTGGTGGTAGGATTGCGGAGACAATTTTGAGTCTGAGATTCTTTATCTTTCAGTATGGCATTGTCTACAAGCTAAATGTGAAAGGAGACAGTACGTCATTGACGGTGTATGGCCTCTCATGGGTTGTTCTGGCAGTGCTTATACTCCTTTTCAAGGTGTTTACCTTCAGCCAGAAGATATCTGTTAACTTCCAGCTTGTGCTGCGTTTCATTCAAGGTGTATCCTTCTTGTTGGCGCTTGCCGGTTTAGCAGTCGCAGTTAAATTTACAGATTTGTCAATTGCAGACGTTTTTGCTTCGATTTTAGCCTTTATACCCACTGGGTGGGGAATCCTTTCTATTGCTATAGCCTGGAAACCGTTGATGAAAAAGCTTCACCTGTGGAAGTCCATTCGTTCCATTGCCCTTCTATATGACGCCGGGATGGGGATGATCATATTCATACCTATTGCATTGTTCTCATGGTTCCCATTTGTATCGACATTCCAAACGCGGCTCATGTTCAACCAGGCTTTCAGCCGAGGGTTGGAGATCTCTCTCGTCCTTGCCGGAAACAACCCGAATACCGGAATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000278 GO:0000280 GO:0001558 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0006996 GO:0007049 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009556 GO:0009846 GO:0009856 GO:0009987 GO:0010769 GO:0012505 GO:0016020 GO:0016043 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022603 GO:0022604 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0033037 GO:0034293 GO:0040007 GO:0040008 GO:0042545 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0043934 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044706 GO:0045229 GO:0045595 GO:0048229 GO:0048232 GO:0048236 GO:0048285 GO:0048589 GO:0048609 GO:0048638 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051179 GO:0051239 GO:0051321 GO:0051510 GO:0051641 GO:0051704 GO:0052386 GO:0052543 GO:0052545 GO:0055047 GO:0060284 GO:0065007 GO:0070727 GO:0071554 GO:0071555 GO:0071840 GO:0071944 GO:0080092 GO:0140014 GO:1903046 GO:1903047 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase

Protein Analysis

2010

Amino Acids

230.85

Weight (kDa)

8.72

Isoelectric Point (pI)

36.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FKS1_dom1	PF14288	442 - 549	9.3e-42	1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1	PF25968	946 - 1123	1.8e-48	Callose synthase 1 domain
Glucan_synthase	PF02364	1130 - 1820	1.2e-232	1,3-beta-glucan synthase component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011696)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07160 AT3G07160 AT3G07160
fragaria_vesca	FvH4_3g44320
malus_domestica	MD11G1016100.v1.1
prunus_persica	Prupe.6G014200_v2.0.a1 Prupe.6G014200_v2.0.a1
pyrus_communis	pycom11g01130
rosa_chinensis	RchiOBHm_Chr5g0080681
rosa_laevigata	RLG00000036885
rosa_roxburghii	Rroxscaffold_1G00002730
rosa_rugosa	Rorug05G0471400 Rorug05G0471400 Rorug05G0471400
rosa_samantha	Rh5AG524500 Rh5BG546700 Rh5CG570000 Rh5DG556900
rosa_wichuraiana	Rw5G048650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2741
AatII	GACGTC	1 cut(s)	4300
AccB7I	CCANNNNNTGG	1 cut(s)	5778
AccI	GTMKAC	3 cut(s)	4686, 5019, 5520
AccII	CGCG	5 cut(s)	16, 40, 61, 1134, 5947
AciI	CCGC	9 cut(s)	38, 59, 721, 799, 899, 1541, 2837, 5471, 5947
AclWI	GGATC	6 cut(s)	125, 488, 545, 558, 1061, 3814
AcoI	YGGCCR	6 cut(s)	56, 391, 2834, 2868, 3631, 5059
AcuI	CTGAAG	8 cut(s)	888, 1000, 1206, 1256, 2333, 3758, 3894, 5605
AcvI	CACGTG	1 cut(s)	3881
AcyI	GRCGYC	4 cut(s)	1972, 4297, 4314, 5871
AdeI	CACNNNGTG	2 cut(s)	1226, 5778
AfiI	CCNNNNNNNGG	9 cut(s)	3229, 3445, 4142, 5575, 5608, 5778, 5880, 5980, 6020
AflII	CTTAAG	1 cut(s)	2975
AflIII	ACRYGT	3 cut(s)	14, 1074, 3917
AgeI	ACCGGT	1 cut(s)	116
AhdI	GACNNNNNGTC	2 cut(s)	3285, 5548
AjiI	CACGTC	2 cut(s)	226, 2258
AloI	GAACNNNNNNTCC	4 cut(s)	4950, 4982, 5100, 5132
Alw21I	GWGCWC	6 cut(s)	1755, 3213, 3543, 3886, 4053, 5239
Alw44I	GTGCAC	2 cut(s)	3209, 4049
AlwI	GGATC	6 cut(s)	125, 488, 545, 558, 1061, 3814
AlwNI	CAGNNNCTG	1 cut(s)	1825
Ama87I	CYCGRG	4 cut(s)	318, 3419, 3852, 4479
ApaLI	GTGCAC	2 cut(s)	3209, 4049
AseI	ATTAAT	1 cut(s)	3128
AsiGI	ACCGGT	1 cut(s)	116
AsiSI	GCGATCGC	1 cut(s)	2200
Asp700I	GAANNNNTTC	7 cut(s)	1738, 2329, 2589, 3796, 4342, 4668, 5788
AspLEI	GCGC	5 cut(s)	1134, 1590, 1656, 2235, 5692
AspS9I	GGNCC	9 cut(s)	913, 1892, 2274, 2683, 3232, 3319, 3702, 4426, 4974
AsuC2I	CCSGG	2 cut(s)	108, 5875
AsuII	TTCGAA	1 cut(s)	1862
AvaI	CYCGRG	4 cut(s)	318, 3419, 3852, 4479
AvaII	GGWCC	7 cut(s)	913, 1892, 2274, 2683, 3232, 3319, 4426
AxyI	CCTNAGG	5 cut(s)	981, 3236, 3315, 3957, 4970
BaeGI	GKGCMC	2 cut(s)	3213, 4053
BalI	TGGCCA	1 cut(s)	2870
BamHI	GGATCC	1 cut(s)	550
BanII	GRGCYC	3 cut(s)	2473, 3543, 4617
BauI	CACGAG	3 cut(s)	4188, 4503, 4577
BbrPI	CACGTG	1 cut(s)	3881
Bbv12I	GWGCWC	6 cut(s)	1755, 3213, 3543, 3886, 4053, 5239
BccI	CCATC	9 cut(s)	327, 367, 1045, 1776, 3554, 4034, 4774, 5222, 5872
BceAI	ACGGC	4 cut(s)	338, 2258, 3618, 5233
BciVI	GTATCC	4 cut(s)	2144, 2157, 4301, 5686
BclI	TGATCA	2 cut(s)	4282, 5886
BcnI	CCSGG	2 cut(s)	108, 5875
BfaI	CTAG	8 cut(s)	273, 344, 750, 1976, 2460, 2853, 4394, 4943
BfmI	CTRYAG	7 cut(s)	1215, 3456, 3963, 4926, 5003, 5143, 5799
BfoI	RGCGCY	1 cut(s)	5693
BfrI	CTTAAG	1 cut(s)	2975
BfuI	GTATCC	4 cut(s)	2144, 2157, 4301, 5686
BglII	AGATCT	3 cut(s)	2497, 3193, 5985
BlpI	GCTNAGC	2 cut(s)	3537, 4398
BmcAI	AGTACT	2 cut(s)	1013, 1613
Bme18I	GGWCC	7 cut(s)	913, 1892, 2274, 2683, 3232, 3319, 4426
BmeRI	GACNNNNNGTC	2 cut(s)	3285, 5548
BmeT110I	CYCGRG	4 cut(s)	318, 3419, 3852, 4479
BmgBI	CACGTC	2 cut(s)	226, 2258
BmgT120I	GGNCC	9 cut(s)	913, 1892, 2274, 2683, 3232, 3319, 3702, 4426, 4974
BmiI	GGNNCC	6 cut(s)	552, 640, 767, 2472, 3321, 5921
BmrI	ACTGGG	1 cut(s)	5784
BmuI	ACTGGG	1 cut(s)	5784
BplI	GAGNNNNNCTC	2 cut(s)	4049, 4081
BpmI	CTGGAG	7 cut(s)	258, 2042, 2637, 3185, 3422, 3527, 3822
Bpu1102I	GCTNAGC	2 cut(s)	3537, 4398
Bpu14I	TTCGAA	1 cut(s)	1862
BpuEI	CTTGAG	4 cut(s)	1265, 1940, 2708, 3707
BpuMI	CCSGG	2 cut(s)	108, 5875
BsaAI	YACGTR	1 cut(s)	3881
BsaHI	GRCGYC	4 cut(s)	1972, 4297, 4314, 5871
BsaI	GGTCTC	1 cut(s)	234
BsaWI	WCCGGW	4 cut(s)	97, 116, 4156, 6020
BsaXI	ACNNNNNCTCC	2 cut(s)	189, 219
Bsc4I	CCNNNNNNNGG	9 cut(s)	3229, 3445, 4142, 5575, 5608, 5778, 5880, 5980, 6020
Bse118I	RCCGGY	4 cut(s)	54, 116, 393, 5696
Bse21I	CCTNAGG	5 cut(s)	981, 3236, 3315, 3957, 4970
Bse3DI	GCAATG	6 cut(s)	660, 909, 1627, 1914, 3981, 5853
BseLI	CCNNNNNNNGG	9 cut(s)	3229, 3445, 4142, 5575, 5608, 5778, 5880, 5980, 6020
BseMI	GCAATG	6 cut(s)	660, 909, 1627, 1914, 3981, 5853
BseSI	GKGCMC	2 cut(s)	3213, 4053
BseX3I	CGGCCG	1 cut(s)	56
BseYI	CCCAGC	1 cut(s)	5412
BsgI	GTGCAG	1 cut(s)	3195
Bsh1236I	CGCG	5 cut(s)	16, 40, 61, 1134, 5947
Bsh1285I	CGRYCG	2 cut(s)	59, 2200
BshTI	ACCGGT	1 cut(s)	116
BsiEI	CGRYCG	2 cut(s)	59, 2200
BsiHKAI	GWGCWC	6 cut(s)	1755, 3213, 3543, 3886, 4053, 5239
BsiHKCI	CYCGRG	4 cut(s)	318, 3419, 3852, 4479
BslFI	GGGAC	4 cut(s)	1225, 1836, 4614, 4916
BslI	CCNNNNNNNGG	9 cut(s)	3229, 3445, 4142, 5575, 5608, 5778, 5880, 5980, 6020
BsmBI	CGTCTC	1 cut(s)	4674
BsmFI	GGGAC	4 cut(s)	1225, 1836, 4614, 4916
BsmI	GAATGC	3 cut(s)	1278, 1397, 1838
Bso31I	GGTCTC	1 cut(s)	234
BsoBI	CYCGRG	4 cut(s)	318, 3419, 3852, 4479
Bsp119I	TTCGAA	1 cut(s)	1862
Bsp1286I	GDGCHC	8 cut(s)	1755, 2473, 3213, 3543, 3886, 4053, 4617, 5239
Bsp1407I	TGTACA	3 cut(s)	690, 3033, 4760
Bsp1720I	GCTNAGC	2 cut(s)	3537, 4398
Bsp19I	CCATGG	1 cut(s)	4977
BspACI	CCGC	9 cut(s)	38, 59, 721, 799, 899, 1541, 2837, 5471, 5947
BspFNI	CGCG	5 cut(s)	16, 40, 61, 1134, 5947
BspLI	GGNNCC	6 cut(s)	552, 640, 767, 2472, 3321, 5921
BspMAI	CTGCAG	5 cut(s)	1219, 3460, 3967, 4930, 5007
BspPI	GGATC	6 cut(s)	125, 488, 545, 558, 1061, 3814
BspQI	GCTCTTC	1 cut(s)	4005
BspT104I	TTCGAA	1 cut(s)	1862
BspTI	CTTAAG	1 cut(s)	2975
BspTNI	GGTCTC	1 cut(s)	234
BsrDI	GCAATG	6 cut(s)	660, 909, 1627, 1914, 3981, 5853
BsrFI	RCCGGY	4 cut(s)	54, 116, 393, 5696
BsrGI	TGTACA	3 cut(s)	690, 3033, 4760
BssAI	RCCGGY	4 cut(s)	54, 116, 393, 5696
BssNAI	GTATAC	1 cut(s)	4687
BssNI	GRCGYC	4 cut(s)	1972, 4297, 4314, 5871
BssSI	CACGAG	3 cut(s)	4188, 4503, 4577
BssT1I	CCWWGG	7 cut(s)	1314, 1649, 1888, 2823, 2871, 4558, 4977
Bst1107I	GTATAC	1 cut(s)	4687
Bst2BI	CACGAG	3 cut(s)	4188, 4503, 4577
Bst6I	CTCTTC	6 cut(s)	864, 2058, 2640, 2652, 3059, 4005
BstACI	GRCGYC	4 cut(s)	1972, 4297, 4314, 5871
BstAFI	CTTAAG	1 cut(s)	2975
BstAUI	TGTACA	3 cut(s)	690, 3033, 4760
BstBAI	YACGTR	1 cut(s)	3881
BstBI	TTCGAA	1 cut(s)	1862
BstDSI	CCRYGG	2 cut(s)	20, 4977
BstEII	GGTNACC	2 cut(s)	119, 4442
BstENI	CCTNNNNNAGG	1 cut(s)	5606
BstFNI	CGCG	5 cut(s)	16, 40, 61, 1134, 5947
BstH2I	RGCGCY	1 cut(s)	5693
BstHHI	GCGC	5 cut(s)	1134, 1590, 1656, 2235, 5692
BstMCI	CGRYCG	2 cut(s)	59, 2200
BstNSI	RCATGY	4 cut(s)	401, 527, 1078, 4328
BstPI	GGTNACC	2 cut(s)	119, 4442
BstSFI	CTRYAG	7 cut(s)	1215, 3456, 3963, 4926, 5003, 5143, 5799
BstSLI	GKGCMC	2 cut(s)	3213, 4053
BstUI	CGCG	5 cut(s)	16, 40, 61, 1134, 5947
BstX2I	RGATCY	7 cut(s)	130, 493, 550, 1066, 2497, 3193, 5985
BstXI	CCANNNNNNTGG	2 cut(s)	3400, 3606
BstYI	RGATCY	7 cut(s)	130, 493, 550, 1066, 2497, 3193, 5985
BstZ17I	GTATAC	1 cut(s)	4687
BstZI	CGGCCG	1 cut(s)	56
Bsu36I	CCTNAGG	5 cut(s)	981, 3236, 3315, 3957, 4970
BsuI	GTATCC	4 cut(s)	2144, 2157, 4301, 5686
BtgI	CCRYGG	2 cut(s)	20, 4977
BtgZI	GCGATG	1 cut(s)	346
BtrI	CACGTC	2 cut(s)	226, 2258
BtsI	GCAGTG	3 cut(s)	1002, 4923, 5595
CaiI	CAGNNNCTG	1 cut(s)	1825
CfoI	GCGC	5 cut(s)	1134, 1590, 1656, 2235, 5692
Cfr10I	RCCGGY	4 cut(s)	54, 116, 393, 5696
Cfr13I	GGNCC	9 cut(s)	913, 1892, 2274, 2683, 3232, 3319, 3702, 4426, 4974
CseI	GACGC	8 cut(s)	5, 29, 1313, 1980, 3420, 3848, 4303, 5879
CspAI	ACCGGT	1 cut(s)	116
CspCI	CAANNNNNGTGG	2 cut(s)	1775, 1810
DraIII	CACNNNGTG	2 cut(s)	1226, 5778
DriI	GACNNNNNGTC	2 cut(s)	3285, 5548
EaeI	YGGCCR	6 cut(s)	56, 391, 2834, 2868, 3631, 5059
EagI	CGGCCG	1 cut(s)	56
Eam1104I	CTCTTC	6 cut(s)	864, 2058, 2640, 2652, 3059, 4005
Eam1105I	GACNNNNNGTC	2 cut(s)	3285, 5548
EarI	CTCTTC	6 cut(s)	864, 2058, 2640, 2652, 3059, 4005
Ecl136II	GAGCTC	1 cut(s)	3541
EclXI	CGGCCG	1 cut(s)	56
Eco130I	CCWWGG	7 cut(s)	1314, 1649, 1888, 2823, 2871, 4558, 4977
Eco24I	GRGCYC	3 cut(s)	2473, 3543, 4617
Eco31I	GGTCTC	1 cut(s)	234
Eco32I	GATATC	5 cut(s)	3111, 3682, 4087, 4528, 5634
Eco47I	GGWCC	7 cut(s)	913, 1892, 2274, 2683, 3232, 3319, 4426
Eco52I	CGGCCG	1 cut(s)	56
Eco53kI	GAGCTC	1 cut(s)	3541
Eco57I	CTGAAG	8 cut(s)	888, 1000, 1206, 1256, 2333, 3758, 3894, 5605
Eco72I	CACGTG	1 cut(s)	3881
Eco81I	CCTNAGG	5 cut(s)	981, 3236, 3315, 3957, 4970
Eco88I	CYCGRG	4 cut(s)	318, 3419, 3852, 4479
Eco91I	GGTNACC	2 cut(s)	119, 4442
EcoICRI	GAGCTC	1 cut(s)	3541
EcoNI	CCTNNNNNAGG	1 cut(s)	5606
EcoO109I	RGGNCCY	3 cut(s)	913, 2683, 3319
EcoO65I	GGTNACC	2 cut(s)	119, 4442
EcoRI	GAATTC	2 cut(s)	3135, 4271
EcoRV	GATATC	5 cut(s)	3111, 3682, 4087, 4528, 5634
EcoT14I	CCWWGG	7 cut(s)	1314, 1649, 1888, 2823, 2871, 4558, 4977
EcoT22I	ATGCAT	2 cut(s)	1280, 4437
EcoT38I	GRGCYC	3 cut(s)	2473, 3543, 4617
ErhI	CCWWGG	7 cut(s)	1314, 1649, 1888, 2823, 2871, 4558, 4977
Esp3I	CGTCTC	1 cut(s)	4674
FalI	AAGNNNNNCTT	8 cut(s)	2895, 2927, 2969, 3001, 4330, 4362, 5175, 5207
FaqI	GGGAC	4 cut(s)	1225, 1836, 4614, 4916
FauI	CCCGC	1 cut(s)	714
FauNDI	CATATG	3 cut(s)	1416, 2125, 3907
FbaI	TGATCA	2 cut(s)	4282, 5886
FblI	GTMKAC	3 cut(s)	4686, 5019, 5520
FriOI	GRGCYC	3 cut(s)	2473, 3543, 4617
FspBI	CTAG	8 cut(s)	273, 344, 750, 1976, 2460, 2853, 4394, 4943
GlaI	GCGC	5 cut(s)	1133, 1589, 1655, 2234, 5691
GsaI	CCCAGC	1 cut(s)	5416
GsuI	CTGGAG	7 cut(s)	258, 2042, 2637, 3185, 3422, 3527, 3822
HaeII	RGCGCY	1 cut(s)	5693
HgaI	GACGC	8 cut(s)	5, 29, 1313, 1980, 3420, 3848, 4303, 5879
HhaI	GCGC	5 cut(s)	1134, 1590, 1656, 2235, 5692
Hin1I	GRCGYC	4 cut(s)	1972, 4297, 4314, 5871
Hin6I	GCGC	5 cut(s)	1132, 1588, 1654, 2233, 5690
HinP1I	GCGC	5 cut(s)	1132, 1588, 1654, 2233, 5690
HincII	GTYRAC	6 cut(s)	1724, 3363, 4770, 4842, 4878, 5641
HindII	GTYRAC	6 cut(s)	1724, 3363, 4770, 4842, 4878, 5641
HindIII	AAGCTT	6 cut(s)	617, 1853, 4242, 4381, 5418, 5825
HpaI	GTTAAC	3 cut(s)	3363, 4770, 5641
Hpy99I	CGWCG	3 cut(s)	230, 1974, 4299
HpyCH4IV	ACGT	8 cut(s)	225, 2257, 3880, 3917, 4297, 4683, 5549, 5742
HpySE526I	ACGT	8 cut(s)	225, 2257, 3880, 3917, 4297, 4683, 5549, 5742
Hsp92I	GRCGYC	4 cut(s)	1972, 4297, 4314, 5871
HspAI	GCGC	5 cut(s)	1132, 1588, 1654, 2233, 5690
KroI	GCCGGC	1 cut(s)	393
KroNI	GCCGGC	1 cut(s)	395
Ksp22I	TGATCA	2 cut(s)	4282, 5886
KspAI	GTTAAC	3 cut(s)	3363, 4770, 5641
LguI	GCTCTTC	1 cut(s)	4005
LmnI	GCTCC	9 cut(s)	337, 844, 952, 1293, 2476, 2549, 2618, 3546, 5302
MaeI	CTAG	8 cut(s)	273, 344, 750, 1976, 2460, 2853, 4394, 4943
MaeII	ACGT	8 cut(s)	225, 2257, 3880, 3917, 4297, 4683, 5549, 5742
MfeI	CAATTG	3 cut(s)	4176, 4209, 5733
MflI	RGATCY	7 cut(s)	130, 493, 550, 1066, 2497, 3193, 5985
MhlI	GDGCHC	8 cut(s)	1755, 2473, 3213, 3543, 3886, 4053, 4617, 5239
MlsI	TGGCCA	1 cut(s)	2870
MluI	ACGCGT	1 cut(s)	14
MluNI	TGGCCA	1 cut(s)	2870
MlyI	GAGTC	6 cut(s)	256, 755, 3647, 4564, 4841, 5494
MmeI	TCCRAC	4 cut(s)	1919, 2233, 4318, 5961
Mox20I	TGGCCA	1 cut(s)	2870
Mph1103I	ATGCAT	2 cut(s)	1280, 4437
MroNI	GCCGGC	1 cut(s)	393
MroXI	GAANNNNTTC	7 cut(s)	1738, 2329, 2589, 3796, 4342, 4668, 5788
MscI	TGGCCA	1 cut(s)	2870
MslI	CAYNNNNRTG	3 cut(s)	1940, 4449, 5079
Msp20I	TGGCCA	1 cut(s)	2870
MspA1I	CMGCKG	2 cut(s)	850, 5002
MspCI	CTTAAG	1 cut(s)	2975
MunI	CAATTG	3 cut(s)	4176, 4209, 5733
Mva1269I	GAATGC	3 cut(s)	1278, 1397, 1838
MvnI	CGCG	5 cut(s)	16, 40, 61, 1134, 5947
NaeI	GCCGGC	1 cut(s)	395
NciI	CCSGG	2 cut(s)	108, 5875
NcoI	CCATGG	1 cut(s)	4977
NdeI	CATATG	3 cut(s)	1416, 2125, 3907
NgoMIV	GCCGGC	1 cut(s)	393
NlaIV	GGNNCC	6 cut(s)	552, 640, 767, 2472, 3321, 5921
NmeAIII	GCCGAG	4 cut(s)	233, 244, 1150, 5999
NsiI	ATGCAT	2 cut(s)	1280, 4437
NspI	RCATGY	4 cut(s)	401, 527, 1078, 4328
NspV	TTCGAA	1 cut(s)	1862
PaeI	GCATGC	2 cut(s)	401, 527
PaeR7I	CTCGAG	4 cut(s)	318, 3419, 3852, 4479
PciI	ACATGT	1 cut(s)	1074
PciSI	GCTCTTC	1 cut(s)	4005
PctI	GAATGC	3 cut(s)	1278, 1397, 1838
PdiI	GCCGGC	1 cut(s)	395
PdmI	GAANNNNTTC	7 cut(s)	1738, 2329, 2589, 3796, 4342, 4668, 5788
PflMI	CCANNNNNTGG	1 cut(s)	5778
PfoI	TCCNGGA	3 cut(s)	2058, 2614, 2955
PinAI	ACCGGT	1 cut(s)	116
Ple19I	CGATCG	1 cut(s)	2200
PleI	GAGTC	6 cut(s)	256, 754, 3647, 4564, 4840, 5493
PmaCI	CACGTG	1 cut(s)	3881
PmlI	CACGTG	1 cut(s)	3881
PpsI	GAGTC	6 cut(s)	256, 754, 3647, 4564, 4840, 5493
Ppu21I	YACGTR	1 cut(s)	3881
PpuMI	RGGWCCY	3 cut(s)	913, 2683, 3319
PscI	ACATGT	1 cut(s)	1074
PshBI	ATTAAT	1 cut(s)	3128
PsiI	TTATAA	1 cut(s)	2741
Psp124BI	GAGCTC	1 cut(s)	3543
Psp5II	RGGWCCY	3 cut(s)	913, 2683, 3319
PspCI	CACGTG	1 cut(s)	3881
PspEI	GGTNACC	2 cut(s)	119, 4442
PspFI	CCCAGC	1 cut(s)	5412
PspN4I	GGNNCC	6 cut(s)	552, 640, 767, 2472, 3321, 5921
PspPI	GGNCC	9 cut(s)	913, 1892, 2274, 2683, 3232, 3319, 3702, 4426, 4974
PspPPI	RGGWCCY	3 cut(s)	913, 2683, 3319
PspXI	VCTCGAGB	3 cut(s)	3419, 3852, 4479
PstI	CTGCAG	5 cut(s)	1219, 3460, 3967, 4930, 5007
PstNI	CAGNNNCTG	1 cut(s)	1825
PsuI	RGATCY	7 cut(s)	130, 493, 550, 1066, 2497, 3193, 5985
PvuI	CGATCG	1 cut(s)	2200
PvuII	CAGCTG	2 cut(s)	850, 5002
RgaI	GCGATCGC	1 cut(s)	2200
RseI	CAYNNNNRTG	3 cut(s)	1940, 4449, 5079
SacI	GAGCTC	1 cut(s)	3543
SapI	GCTCTTC	1 cut(s)	4005
Sau96I	GGNCC	9 cut(s)	913, 1892, 2274, 2683, 3232, 3319, 3702, 4426, 4974
ScaI	AGTACT	2 cut(s)	1013, 1613
SchI	GAGTC	6 cut(s)	256, 755, 3647, 4564, 4841, 5494
SduI	GDGCHC	8 cut(s)	1755, 2473, 3213, 3543, 3886, 4053, 4617, 5239
SfaAI	GCGATCGC	1 cut(s)	2200
SfcI	CTRYAG	7 cut(s)	1215, 3456, 3963, 4926, 5003, 5143, 5799
Sfr274I	CTCGAG	4 cut(s)	318, 3419, 3852, 4479
SfuI	TTCGAA	1 cut(s)	1862
SgfI	GCGATCGC	1 cut(s)	2200
SinI	GGWCC	7 cut(s)	913, 1892, 2274, 2683, 3232, 3319, 4426
SlaI	CTCGAG	4 cut(s)	318, 3419, 3852, 4479
SmiMI	CAYNNNNRTG	3 cut(s)	1940, 4449, 5079
SmlI	CTYRAG	9 cut(s)	318, 1244, 1919, 2687, 2975, 3419, 3686, 3852, 4479
SmoI	CTYRAG	9 cut(s)	318, 1244, 1919, 2687, 2975, 3419, 3686, 3852, 4479
SphI	GCATGC	2 cut(s)	401, 527
SsiI	CCGC	9 cut(s)	38, 59, 721, 799, 899, 1541, 2837, 5471, 5947
SspI	AATATT	4 cut(s)	931, 1375, 2997, 3573
SspMI	CTAG	8 cut(s)	273, 344, 750, 1976, 2460, 2853, 4394, 4943
SstI	GAGCTC	1 cut(s)	3543
StyI	CCWWGG	7 cut(s)	1314, 1649, 1888, 2823, 2871, 4558, 4977
TaiI	ACGT	8 cut(s)	228, 2260, 3883, 3920, 4300, 4686, 5552, 5745
TaqII	GACCGA	2 cut(s)	258, 923
TatI	WGTACW	7 cut(s)	690, 1011, 1344, 1611, 3033, 4581, 4760
TauI	GCSGC	4 cut(s)	61, 902, 2839, 5950
TspGWI	ACGGA	5 cut(s)	338, 2712, 3461, 4418, 5304
Van91I	CCANNNNNTGG	1 cut(s)	5778
Vha464I	CTTAAG	1 cut(s)	2975
VneI	GTGCAC	2 cut(s)	3209, 4049
VpaK11BI	GGWCC	7 cut(s)	913, 1892, 2274, 2683, 3232, 3319, 4426
VspI	ATTAAT	1 cut(s)	3128
XagI	CCTNNNNNAGG	1 cut(s)	5606
XceI	RCATGY	4 cut(s)	401, 527, 1078, 4328
XcmI	CCANNNNNNNNNTGG	4 cut(s)	1581, 2779, 4283, 5272
XhoI	CTCGAG	4 cut(s)	318, 3419, 3852, 4479
XmiI	GTMKAC	3 cut(s)	4686, 5019, 5520
XmnI	GAANNNNTTC	7 cut(s)	1738, 2329, 2589, 3796, 4342, 4668, 5788
XspI	CTAG	8 cut(s)	273, 344, 750, 1976, 2460, 2853, 4394, 4943
ZraI	GACGTC	1 cut(s)	4298
ZrmI	AGTACT	2 cut(s)	1013, 1613
Zsp2I	ATGCAT	2 cut(s)	1280, 4437

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.