Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0011696

Physical Location & Seq

Genomic Coordinates Forward (+)

86866097 .. 86889917

Gene Structure 23821 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG546700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5715 bp

ATGGCTCAAGTTGAGGATCGATGGGAGCGGTTAGTCCGTGCAGTATTGAGGAGGGAAAGGATGGGGCCTGATGCATATGAGCGGCATGGGACTGGCATTGCCGGAAATGTTCCATCTTCACTTGAAAATAATAGGGATATTGATGAAATTCTCAGAGTTGCAGATGAAATCCAAGATGAAGATCCCAATATCTCTAGAATCCTATGTGAGCATGGTTATTCGTTGGCTCAAAATCTAGACCCCAATAGTGAAGGAAGGGGAGTATTACAGTTCAAGACTGGGTTGATGTCTGTCATTAAGCAAAAGCTAGCGAAGAGGGAAGGGGCAACCATAGATAGAAGCCAGGACATTGTTCGCTTACAAGAGTTCTATAAACAGTATAGACAGAAAAATAATGTTGAACAATTGCGTGAGGAAGAAATGAAGTTGAGGGAGTCTGGGGTTTTCAGTGGAAACCTGGGCGAACTGGAAAGGAAAACAGTGAAGAGGAAAAGAGTATTCGCGACTCTAAGAGTTCTGGGCAAAGTTTTGGAGCAGCTGACAGAGGACATTCCAGAAGAGCTCAAGCGCCAAATGGAGATAGATGCTGCAATGACTGAAGACCTGATTGCGTACAATATTATTCCACTGGATGCTCCTAGCATTACAAATGTTATCATGTCCTTGGCTGAGGTTCAAGCAGCAGTTTCTGGGTTAAAGTACTTCAGGGGCCTACCAAAGTTACCGACAGATTTTCCGATTCCTGCTACACGAGAGCCTGACATGCTTGATTTCCTGCATTATGTATTTGGATTTCAGAAAGACAATGTTTGTAACCAGCGCGAACATATTGTGCATCTCCTTGCAAATGAGCAATCACGGCTTCGTATTCCTGAAGAAACTGAACCTATATTGGATGAGGCTGCGGTGCAAAATATATTTTTAAAGTCCCTAGACAACTATATTAAGTGGTGTGACTACTTGTGCATTCAGCCAGTTTGGAGCAATTTGCAGTCAGTTAGCAAGGAGAAAAAGCTACTGTTTGTTTCCATGTACTGTTTGATCTGGGGTGAAGCAGCCAATGTTAGATTTCTTCCAGAATGCTTGTGCTACATATTTCATCATATGGCAAGGGAAATGGATGAAATTCTGAGACAGCAGACTGCACAGCCAGCCAACAGTTGTAATATTGAAAATAGAGTTTCTTTCCTTGATCACGTTATATATCCTCTGTTTGAGATTGTGTCTGCGGAAGCTGGAAACAATGAAAATGGACGAGCACCACATTCTGCTTGGCGCAATTATGATGATTTTAATGAGTACTTCTGGTCACTCCATTGTTTTGAACTTAGTTGGCCCTGGCGCAAAGGTTCATCATTTTTTCAAAAGCCAACGCCAAGGTCAAAGAATATTCTCAAATCTGGCAGAAGTCAACATCGTGGAAAAACTTCATTTGTTGAGCATAGGACATTTCTCCATCTGTATCATAGTTTCCACCGTCTGTGGATATTCCTTGTGATGATGTTTCAGGGACTTGCGATCATTGCATTCAATGGAAGTTTTGATGCAAAATGTATTAGAGAAATTCTCAGTCTCGGTCCGACATTTGTTGGAATGAAATTTCTCGAGAGTGTTCTTGATGTTCTTATGATGTATGGTGCCTATTCTACAACACGGCGCCTAGCAGTTTCTCGAATTTTTCTCCGATTTGTTTGGTTTGGCACTGCATCAGTGGTCATATCTTTTCTTTATGTGAAAGCTCTCCAGGAAGAGAGTAAACAAAATGGAAACCCTATTATCTACAAGTTGTATCTGCTCATTGTAGGCATATATGCTGGTATCCAGTTCTTCATTAGTTTTTTCATGCGCATACCAGCCTGCCACAATCTGACTAATCAATGTGATCGCTGGTCTCTGATTCGCTTTGTCAAGTGGATGCGCCAGGAACGGTACTATGTTGGACGGGGCATGTATGAGAGGACTACTGATTTCATCAAGTACATGTTTTTCTGGCTTGTGATTTTGAGTGGAAAGTTTTCATTTGCATACTTTCTCCAGATCAAGCCGTTGGTGGAACCAACCAGAGTTATAGTGAACACAAATGCTATAACGTATACTTGGCATGATTTTGTTTCTAAAAACAACTATAATGCTCTGACAGTTGCTGCTTTGTGGGCTCCCGTGATAGTTATTTATCTTTTAGATCTCTATGTATTTTACACCGTTATATCTGCTGTGTGGGGATTCTTGCTTGGTGCCAGAGATCGTCTTGGAGAGATTAGATCATTGGAAGCACTTCATAAACTATTTGAGCAGTTTCCTGGAGCTTTTATGGACACTCTTCATATCCGCCTGCCTAACAGGGCTTACCATCAGTCTTCCAGTGAGGACATTGAGAAGAACAAGGTTGATGCTAGTCGATTTTCTCCCTTTTGGAATGAAATCATAAAAAATCTGAGGGAAGAAGATTATATCACTGACCTAGAAATGGAGCTGCTTGTAATGCCTAAAAATTCTGGAAGTCTTCCCTTAGTTCAGTGGCCCCTTTTCCTTCTTGCTAGCAAGATATTTGTTGCCAAAGATATTGCTCTTGAAAGCAGGGATTCACAAGATGAGCTTTGGGAAAGAATTTCTAGAGATGACTATATGAAGTATGCTGTCCAGGAGTGCTTCCATACCATTAAGCTTATCCTAAGTGAAATATTGGAGGGTGAAGGAAAGCTGTGGGTTGAAAGGATATATGATGATATTCAAGTAAGCATAGTGAAGAAGAGTATTCAGGCTGACTTTCAACTGAACAAGCTGCCACTCGTGATTTCAAGAGTTACTGCATTAATGGGAATTCTGAAAAAGGAGGAAACATCTGCACTGGTGGATGGTGCTATTAAGGCTGTTCAAGATCTCTACGACATTGTGCGCCATGATGTCCTTTCTATTAACATGAGGGAAAACTATGAAACGTGGAATTTGTTGTCAAAAGCACGGAAGGATGGTCGGTTGTTTGCCAAGTTGAAGTGGCCCAAGGATCCTTCATTGAGAGCGCAAGTCAAAAGATTGTATTCATTGTTGACAATTCAAGATTCAGCTGCCAATGTCCCCCAAAATCTGGAGGCCGGACGTAGGCTGCAGTTCTTTACAAATTCTCTTTTCATGGAGATGCCCGAAGCAAGGCCAGTCCGTGAAATGTTGTCGTTCAGTGTTTTTACTCCATATTATGCTGAGACTGTGCTCTACAGTATCGCTGAACTCCAGAAGAAAAATGAAGATGGGATATCAGTATTGTTTTACCTTCAAAAGATTTACCCAGATGAGTGGAAGAACTTTCTTTTACGGATTGGCCGTGACGAAAATGCACATGACTCGGAACTTTATGATAATCCCTCTGATATTCTTGAGCTTCGGTTTTGGGCGTCTTACCGAGGCCAAACATTGGCCAGAACTGTTCGTGGGATGATGTATTATAGGAAGGCTCTTATGCTTCAGACCTATTTGGAGAGATTGAATTCTGGAGTATCAGATGTGGAGGCTGCAATTTCTAGTGGTGATGCGGCTGAAACTCGAGCATTTGAGTTATCTCCTGAAGCACGAGCTCAGGCAGACCTGAAGTTTACATATGTAGTTACTTGTCAAATATATGGAAAACAGAAAGAAGGCCAAAAACCTGAGGCTGCTGATATAGCATTGCTTATGCAAAGAAATGAGGCTCTTCGAGTTGCTTTTATCGATGAGGTTGAGACTTTGAAGGATGGTAAAGTGAACAAAGAGTTCTATTCAAAACTTGTAAAAGCAGATATCAATGGGAAGGACAAGGAAATCTACTCTATAAAACTGCCTGGAAATCCAAAAATAGGTGAAGGGAAACCGGAGAACCAAAATCATGCAATTGTATTTACTCGTGGAAATGCAATTCAGACAATTGACATGAATCAGGATAACTATTTTGAGGAAGCTTTGAAGATGAGAAATTTACTCGAAGAATTCCACCGTGATCATGGTATCCGTCATCCTACAATTCTTGGTGTCAGGGAACATGTCTTTACGGGAAGTGTTTCTTCTTTAGCGTCATTCATGTCCCATCAGGAAACAAGCTTTGTAACTCTAGCTCAGCGGGTTCTTGCGAATCCTTTGAAGGTCCGTATGCATTATGGTCATCCTGATGTGTTTGATAGAGTTTTCCACATAACACGGGGTGGTATTAGCAAGGCTTCTCGAGTGATTAACATCAGTGAAGACATCTTTGCAGGCTTCAACTCAACTTTACGCCAAGGAAATGTGACTCACCATGAGTATATTCAGGTTGGCAAAGGGCGGGACGTTGGGCTCAACCAAATTGCAGTATTTGAGGGGAAGGTTGCTGGTGGTAATGGTGAACAGGTTCTGAGTCGTGATGTATACAGGCTTGGGCAGCAGTTTGATTTCTTCCGCATGCTGTCATTTTACTTTACCACTGTCGGCTACTACTTCTGTACAATGTTAACAGTGCTGACGGTTTACATTTTTCTCTACGGGAAAACATATATGGCGCTATCTGGGGTTGGTGAATACTTGCAAAACGTAGCTGAGGTAACCAAGAATACCGCACTGACTGCTGCTCTAAATACACAATTCCTCCTTCAAATTGGTATCTTTACTGCTGTCCCGATGATTTTAGGCTCCATCTTGGAACAGGGTTTCCTGCGGGCCATAGTAAGTTTCCTCACAATGCAGTTGCAACTTTGTTCTGTCTTCTTCACATTCTCCTTGGGAACCAAAACACATTATTTTGGCCGGACAATTCTCCATGGTGGTGCAAAGTATCAAGCAACCGGCAGGGGATTTGTAGTCCGGCATGTTAAGTTTACAGAGAATTACAGGCTTTACTCCCGCAGTCATTTTACCAAAGGGTTGGAAGTAGTTCTTTTGTTGGTAGTGTACCTTGCTTATGGATATGATGATGGCGGTGCTATATCATACATTCTTCTTTCAATAACCAGTTGGTTCATGGCTCTTTCTTGGCTTTTTGCTCCCTATTTGTTCAACCCATCTGGGTTTGAGTGGCAGAAGGTTGTGGAGGATTTCCGAGATTGGACAAATTGGCTTCTTTATAGAGGTGGAATTGGAGTTAAGGGTGAAGAAAGCTGGGAAGCTTGGTGGGAGGAAGAACTGGCGCATATTAGAACCTTGGGAGGAAGGATTGCAGAGACAATTTTGAGTCTAAGATTCTTTATCTTCCAGTATGGAATTATCTACCGGCTAGATGTGAAGGGATCAGATACATCACTAACGGTGTATGGCCTCTCATGGATCGTGCTGGTGGTGCTTATAATTCTTTTCAAGGTTTTCACCTTCAGCCAGAAGATATCTGTCAACTTCCAGCTTCTGTTGCGCTTCATTCAAGGGGTCGCCTTCATGTTGGCACTGGCTGGTTTAGCAGTAGCAGTCAAATTTACAGATCTGACAATCGCAGACGTTTTTGCTTCCATTTTAGCGTTTGTACCCACTGGGTGGGGAATTCTTTCCATTTGCATAGCTTGGAAACCCTTGATGAAGAAACTGGGCTTGTGGAAATCCATCCGTTCCATTGCCCTTCTTTATGATGCTGGGATGGGTATGGTTATATTCATCCCCATTGCATTGTCGTCATGGTTCCCATTTGTATCAACGTTCCAAACTCGGCTCATGTTCAACCAGGCTTTCAGTCGAGGGTTGGAAATCTCTGTACTACTTGCTGGAAACAACCCCAATTCTGGATTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000278 GO:0000280 GO:0001558 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0006996 GO:0007049 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009556 GO:0009846 GO:0009856 GO:0009987 GO:0010769 GO:0012505 GO:0016020 GO:0016043 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022603 GO:0022604 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0033037 GO:0034293 GO:0040007 GO:0040008 GO:0042545 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0043934 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044706 GO:0045229 GO:0045595 GO:0048229 GO:0048232 GO:0048236 GO:0048285 GO:0048589 GO:0048609 GO:0048638 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051179 GO:0051239 GO:0051321 GO:0051510 GO:0051641 GO:0051704 GO:0052386 GO:0052543 GO:0052545 GO:0055047 GO:0060284 GO:0065007 GO:0070727 GO:0071554 GO:0071555 GO:0071840 GO:0071944 GO:0080092 GO:0140014 GO:1903046 GO:1903047 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase

Protein Analysis

1904

Amino Acids

219.08

Weight (kDa)

8.22

Isoelectric Point (pI)

36.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FKS1_dom1	PF14288	338 - 445	2.5e-40	1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1	PF25968	841 - 1018	4.6e-48	Callose synthase 1 domain
Glucan_synthase	PF02364	1025 - 1715	1.5e-232	1,3-beta-glucan synthase component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011696)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07160 AT3G07160 AT3G07160
fragaria_vesca	FvH4_3g44320
malus_domestica	MD11G1016100.v1.1
prunus_persica	Prupe.6G014200_v2.0.a1 Prupe.6G014200_v2.0.a1
pyrus_communis	pycom11g01130
rosa_chinensis	RchiOBHm_Chr5g0080681
rosa_laevigata	RLG00000036885
rosa_roxburghii	Rroxscaffold_1G00002730
rosa_rugosa	Rorug05G0471400 Rorug05G0471400 Rorug05G0471400
rosa_samantha	Rh5AG524500 Rh5BG546700 Rh5CG570000 Rh5DG556900
rosa_wichuraiana	Rw5G048650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	5282
Acc16I	TGCGCA	1 cut(s)	1847
AccB1I	GGYRCC	3 cut(s)	1637, 1656, 2234
AccB7I	CCANNNNNTGG	3 cut(s)	3085, 3409, 5463
AccBSI	CCGCTC	2 cut(s)	28, 82
AccI	GTMKAC	2 cut(s)	2093, 4371
AccII	CGCG	2 cut(s)	503, 822
AclI	AACGTT	1 cut(s)	5621
AclWI	GGATC	6 cut(s)	24, 176, 2999, 3012, 5233, 5270
AcoI	YGGCCR	3 cut(s)	3316, 3411, 4744
AcuI	CTGAAG	7 cut(s)	618, 688, 894, 3443, 3579, 3602, 5290
AcyI	GRCGYC	2 cut(s)	1657, 3389
AdeI	CACNNNGTG	2 cut(s)	4169, 5463
AfiI	CCNNNNNNNGG	8 cut(s)	2467, 3085, 3099, 3147, 3409, 3827, 5463, 5705
AflIII	ACRYGT	2 cut(s)	1980, 4009
AjnI	CCWGG	9 cut(s)	342, 456, 1337, 1743, 1920, 2299, 2640, 3811, 5646
AloI	GAACNNNNNNTCC	2 cut(s)	4635, 4667
Alw21I	GWGCWC	4 cut(s)	564, 1261, 3210, 3571
Alw26I	GTCTC	6 cut(s)	1126, 1577, 1896, 3194, 3707, 5153
AlwI	GGATC	6 cut(s)	24, 176, 2999, 3012, 5233, 5270
AlwNI	CAGNNNCTG	4 cut(s)	689, 2144, 4357, 5338
Ama87I	CYCGRG	3 cut(s)	1604, 3537, 4188
AseI	ATTAAT	1 cut(s)	2813
Asp700I	GAANNNNTTC	7 cut(s)	1426, 2014, 2274, 4027, 4353, 4872, 5473
AspS9I	GGNCC	8 cut(s)	65, 709, 1335, 1577, 2520, 2997, 4111, 4659
AsuHPI	GGTGA	9 cut(s)	1061, 2702, 3533, 3842, 4250, 4358, 4529, 5099, 5293
AsuNHI	GCTAGC	2 cut(s)	307, 2537
AvaI	CYCGRG	3 cut(s)	1604, 3537, 4188
AvaII	GGWCC	2 cut(s)	1577, 4111
AxyI	CCTNAGG	1 cut(s)	3642
BalI	TGGCCA	1 cut(s)	3413
BamHI	GGATCC	1 cut(s)	3004
BanI	GGYRCC	3 cut(s)	1637, 1656, 2234
BanII	GRGCYC	4 cut(s)	564, 2158, 3571, 4302
BauI	CACGAG	4 cut(s)	750, 2789, 3564, 3873
BbsI	GAAGAC	5 cut(s)	606, 2349, 2495, 4215, 4696
Bbv12I	GWGCWC	4 cut(s)	564, 1261, 3210, 3571
BbvCI	CCTCAGC	2 cut(s)	669, 4539
BceAI	ACGGC	4 cut(s)	875, 1670, 2029, 3303
BcgI	CGANNNNNNTGC	2 cut(s)	3321, 3355
BciT130I	CCWGG	9 cut(s)	344, 458, 1339, 1745, 1922, 2301, 2642, 3813, 5648
BciVI	GTATCC	2 cut(s)	1829, 3986
BclI	TGATCA	2 cut(s)	1192, 3967
BcoDI	GTCTC	6 cut(s)	1126, 1577, 1896, 3194, 3707, 5153
BfmI	CTRYAG	2 cut(s)	3104, 3211
BfoI	RGCGCY	3 cut(s)	571, 1660, 4505
BfuI	GTATCC	2 cut(s)	1829, 3986
BglII	AGATCT	3 cut(s)	2182, 2878, 5410
BlpI	GCTNAGC	1 cut(s)	4083
BmcAI	AGTACT	2 cut(s)	701, 1301
Bme1390I	CCNGG	9 cut(s)	344, 458, 1339, 1745, 1922, 2301, 2642, 3813, 5648
Bme18I	GGWCC	2 cut(s)	1577, 4111
BmeT110I	CYCGRG	3 cut(s)	1604, 3537, 4188
BmgT120I	GGNCC	8 cut(s)	65, 709, 1335, 1577, 2520, 2997, 4111, 4659
BmrFI	CCNGG	9 cut(s)	344, 458, 1339, 1745, 1922, 2301, 2642, 3813, 5648
BmrI	ACTGGG	3 cut(s)	288, 5469, 5522
BmtI	GCTAGC	2 cut(s)	311, 2541
BmuI	ACTGGG	3 cut(s)	288, 5469, 5522
BpiI	GAAGAC	5 cut(s)	606, 2349, 2495, 4215, 4696
BplI	GAGNNNNNCTC	2 cut(s)	1551, 1583
BpmI	CTGGAG	6 cut(s)	1727, 2018, 2322, 3107, 3212, 3507
Bpu10I	CCTNAGC	3 cut(s)	669, 3570, 4539
Bpu1102I	GCTNAGC	1 cut(s)	4083
BpuEI	CTTGAG	2 cut(s)	548, 3392
Bsa29I	ATCGAT	2 cut(s)	19, 3702
BsaBI	GATNNNNATC	2 cut(s)	180, 4199
BsaHI	GRCGYC	2 cut(s)	1657, 3389
BsaI	GGTCTC	1 cut(s)	1896
BsaWI	WCCGGW	1 cut(s)	3841
BsaXI	ACNNNNNCTCC	2 cut(s)	4572, 4602
Bsc4I	CCNNNNNNNGG	8 cut(s)	2467, 3085, 3099, 3147, 3409, 3827, 5463, 5705
Bse118I	RCCGGY	2 cut(s)	4784, 5208
Bse21I	CCTNAGG	1 cut(s)	3642
Bse3DI	GCAATG	6 cut(s)	96, 597, 1521, 3659, 5538, 5586
Bse8I	GATNNNNATC	2 cut(s)	180, 4199
BseBI	CCWGG	9 cut(s)	344, 458, 1339, 1745, 1922, 2301, 2642, 3813, 5648
BseCI	ATCGAT	2 cut(s)	19, 3702
BseJI	GATNNNNATC	2 cut(s)	180, 4199
BseLI	CCNNNNNNNGG	8 cut(s)	2467, 3085, 3099, 3147, 3409, 3827, 5463, 5705
BseMI	GCAATG	6 cut(s)	96, 597, 1521, 3659, 5538, 5586
BseRI	GAGGAG	1 cut(s)	64
BseYI	CCCAGC	2 cut(s)	5097, 5558
BsgI	GTGCAG	3 cut(s)	60, 1128, 2829
Bsh1236I	CGCG	2 cut(s)	503, 822
BshNI	GGYRCC	3 cut(s)	1637, 1656, 2234
BshVI	ATCGAT	2 cut(s)	19, 3702
BsiHKAI	GWGCWC	4 cut(s)	564, 1261, 3210, 3571
BsiHKCI	CYCGRG	3 cut(s)	1604, 3537, 4188
BsiSI	CCGG	7 cut(s)	102, 3093, 3842, 4747, 4785, 4804, 5209
BslFI	GGGAC	7 cut(s)	103, 913, 1524, 3059, 4036, 4304, 4601
BslI	CCNNNNNNNGG	8 cut(s)	2467, 3085, 3099, 3147, 3409, 3827, 5463, 5705
BsmAI	GTCTC	6 cut(s)	1126, 1577, 1896, 3194, 3707, 5153
BsmFI	GGGAC	7 cut(s)	103, 913, 1524, 3059, 4036, 4304, 4601
BsmI	GAATGC	3 cut(s)	966, 1085, 1526
Bso31I	GGTCTC	1 cut(s)	1896
BsoBI	CYCGRG	3 cut(s)	1604, 3537, 4188
Bsp1286I	GDGCHC	6 cut(s)	564, 1261, 2158, 3210, 3571, 4302
Bsp1407I	TGTACA	1 cut(s)	4445
Bsp1720I	GCTNAGC	1 cut(s)	4083
Bsp19I	CCATGG	1 cut(s)	4759
Bsp68I	TCGCGA	1 cut(s)	503
BspDI	ATCGAT	2 cut(s)	19, 3702
BspFNI	CGCG	2 cut(s)	503, 822
BspMAI	CTGCAG	1 cut(s)	3108
BspOI	GCTAGC	2 cut(s)	311, 2541
BspPI	GGATC	6 cut(s)	24, 176, 2999, 3012, 5233, 5270
BspQI	GCTCTTC	2 cut(s)	552, 3690
BspT107I	GGYRCC	3 cut(s)	1637, 1656, 2234
BspTNI	GGTCTC	1 cut(s)	1896
BsrBI	CCGCTC	2 cut(s)	28, 82
BsrDI	GCAATG	6 cut(s)	96, 597, 1521, 3659, 5538, 5586
BsrFI	RCCGGY	2 cut(s)	4784, 5208
BsrGI	TGTACA	1 cut(s)	4445
BssAI	RCCGGY	2 cut(s)	4784, 5208
BssNAI	GTATAC	2 cut(s)	2094, 4372
BssNI	GRCGYC	2 cut(s)	1657, 3389
BssSI	CACGAG	4 cut(s)	750, 2789, 3564, 3873
BssT1I	CCWWGG	7 cut(s)	663, 1376, 3000, 4243, 4719, 4759, 5139
Bst1107I	GTATAC	2 cut(s)	2094, 4372
Bst2BI	CACGAG	4 cut(s)	750, 2789, 3564, 3873
Bst2UI	CCWGG	9 cut(s)	344, 458, 1339, 1745, 1922, 2301, 2642, 3813, 5648
Bst6I	CTCTTC	7 cut(s)	308, 479, 552, 1743, 2325, 2744, 3690
BstACI	GRCGYC	2 cut(s)	1657, 3389
BstAPI	GCANNNNNTGC	1 cut(s)	4565
BstAUI	TGTACA	1 cut(s)	4445
BstC8I	GCNNGC	8 cut(s)	309, 1152, 1858, 2333, 2539, 4222, 4406, 4659
BstDSI	CCRYGG	1 cut(s)	4759
BstEII	GGTNACC	1 cut(s)	4543
BstFNI	CGCG	2 cut(s)	503, 822
BstH2I	RGCGCY	3 cut(s)	571, 1660, 4505
BstMAI	GTCTC	6 cut(s)	1126, 1577, 1896, 3194, 3707, 5153
BstNI	CCWGG	9 cut(s)	344, 458, 1339, 1745, 1922, 2301, 2642, 3813, 5648
BstNSI	RCATGY	6 cut(s)	766, 1951, 1984, 4013, 4408, 4811
BstPI	GGTNACC	1 cut(s)	4543
BstSCI	CCNGG	9 cut(s)	342, 456, 1337, 1743, 1920, 2299, 2640, 3811, 5646
BstSFI	CTRYAG	2 cut(s)	3104, 3211
BstUI	CGCG	2 cut(s)	503, 822
BstV2I	GAAGAC	5 cut(s)	606, 2349, 2495, 4215, 4696
BstX2I	RGATCY	5 cut(s)	181, 2182, 2878, 3004, 5410
BstXI	CCANNNNNNTGG	2 cut(s)	3291, 4864
BstYI	RGATCY	5 cut(s)	181, 2182, 2878, 3004, 5410
BstZ17I	GTATAC	2 cut(s)	2094, 4372
Bsu15I	ATCGAT	2 cut(s)	19, 3702
Bsu36I	CCTNAGG	1 cut(s)	3642
BsuI	GTATCC	2 cut(s)	1829, 3986
BsuTUI	ATCGAT	2 cut(s)	19, 3702
BtgI	CCRYGG	1 cut(s)	4759
BtsI	GCAGTG	1 cut(s)	1701
BtuMI	TCGCGA	1 cut(s)	503
Cac8I	GCNNGC	8 cut(s)	309, 1152, 1858, 2333, 2539, 4222, 4406, 4659
CaiI	CAGNNNCTG	4 cut(s)	689, 2144, 4357, 5338
Cfr10I	RCCGGY	2 cut(s)	4784, 5208
Cfr13I	GGNCC	8 cut(s)	65, 709, 1335, 1577, 2520, 2997, 4111, 4659
ClaI	ATCGAT	2 cut(s)	19, 3702
CpoI	CGGWCCG	1 cut(s)	1577
CseI	GACGC	2 cut(s)	3378, 4029
CspI	CGGWCCG	1 cut(s)	1577
DinI	GGCGCC	1 cut(s)	1658
DraI	TTTAAA	1 cut(s)	924
DraIII	CACNNNGTG	2 cut(s)	4169, 5463
EaeI	YGGCCR	3 cut(s)	3316, 3411, 4744
Eam1104I	CTCTTC	7 cut(s)	308, 479, 552, 1743, 2325, 2744, 3690
EarI	CTCTTC	7 cut(s)	308, 479, 552, 1743, 2325, 2744, 3690
EciI	GGCGGA	1 cut(s)	2318
Ecl136II	GAGCTC	2 cut(s)	562, 3569
Eco130I	CCWWGG	7 cut(s)	663, 1376, 3000, 4243, 4719, 4759, 5139
Eco24I	GRGCYC	4 cut(s)	564, 2158, 3571, 4302
Eco31I	GGTCTC	1 cut(s)	1896
Eco32I	GATATC	3 cut(s)	3252, 3772, 5319
Eco47I	GGWCC	2 cut(s)	1577, 4111
Eco53kI	GAGCTC	2 cut(s)	562, 3569
Eco57I	CTGAAG	7 cut(s)	618, 688, 894, 3443, 3579, 3602, 5290
Eco81I	CCTNAGG	1 cut(s)	3642
Eco88I	CYCGRG	3 cut(s)	1604, 3537, 4188
Eco91I	GGTNACC	1 cut(s)	4543
EcoICRI	GAGCTC	2 cut(s)	562, 3569
EcoO109I	RGGNCCY	2 cut(s)	65, 709
EcoO65I	GGTNACC	1 cut(s)	4543
EcoRI	GAATTC	4 cut(s)	2820, 3481, 3956, 5469
EcoRII	CCWGG	9 cut(s)	342, 456, 1337, 1743, 1920, 2299, 2640, 3811, 5646
EcoRV	GATATC	3 cut(s)	3252, 3772, 5319
EcoT14I	CCWWGG	7 cut(s)	663, 1376, 3000, 4243, 4719, 4759, 5139
EcoT22I	ATGCAT	2 cut(s)	76, 4122
EcoT38I	GRGCYC	4 cut(s)	564, 2158, 3571, 4302
EgeI	GGCGCC	1 cut(s)	1658
EheI	GGCGCC	1 cut(s)	1658
ErhI	CCWWGG	7 cut(s)	663, 1376, 3000, 4243, 4719, 4759, 5139
FalI	AAGNNNNNCTT	6 cut(s)	2580, 2612, 4015, 4047, 4860, 4892
FaqI	GGGAC	7 cut(s)	103, 913, 1524, 3059, 4036, 4304, 4601
FauI	CCCGC	4 cut(s)	4080, 4281, 4650, 4850
FauNDI	CATATG	3 cut(s)	76, 1104, 3592
FbaI	TGATCA	2 cut(s)	1192, 3967
FblI	GTMKAC	2 cut(s)	2093, 4371
FriOI	GRGCYC	4 cut(s)	564, 2158, 3571, 4302
FspAI	RTGCGCAY	1 cut(s)	1847
FspI	TGCGCA	1 cut(s)	1847
GsaI	CCCAGC	2 cut(s)	5101, 5562
GsuI	CTGGAG	6 cut(s)	1727, 2018, 2322, 3107, 3212, 3507
HaeII	RGCGCY	3 cut(s)	571, 1660, 4505
HapII	CCGG	7 cut(s)	102, 3093, 3842, 4747, 4785, 4804, 5209
HgaI	GACGC	2 cut(s)	3378, 4029
Hin1I	GRCGYC	2 cut(s)	1657, 3389
HincII	GTYRAC	4 cut(s)	1412, 3048, 4455, 5326
HindII	GTYRAC	4 cut(s)	1412, 3048, 4455, 5326
HindIII	AAGCTT	4 cut(s)	2663, 3927, 4066, 5103
HpaI	GTTAAC	1 cut(s)	4455
HpaII	CCGG	7 cut(s)	102, 3093, 3842, 4747, 4785, 4804, 5209
HphI	GGTGA	9 cut(s)	1061, 2702, 3533, 3842, 4250, 4358, 4529, 5099, 5293
HpyCH4IV	ACGT	8 cut(s)	1197, 2090, 2939, 3097, 4293, 4533, 5427, 5621
HpySE526I	ACGT	8 cut(s)	1197, 2090, 2939, 3097, 4293, 4533, 5427, 5621
Hsp92I	GRCGYC	2 cut(s)	1657, 3389
KasI	GGCGCC	1 cut(s)	1656
Ksp22I	TGATCA	2 cut(s)	1192, 3967
KspAI	GTTAAC	1 cut(s)	4455
LguI	GCTCTTC	2 cut(s)	552, 3690
LmnI	GCTCC	9 cut(s)	25, 532, 640, 981, 2161, 2303, 2470, 4637, 4987
MaeII	ACGT	8 cut(s)	1197, 2090, 2939, 3097, 4293, 4533, 5427, 5621
MbiI	CCGCTC	2 cut(s)	28, 82
MfeI	CAATTG	3 cut(s)	404, 3861, 3894
MflI	RGATCY	5 cut(s)	181, 2182, 2878, 3004, 5410
MhlI	GDGCHC	6 cut(s)	564, 1261, 2158, 3210, 3571, 4302
MlsI	TGGCCA	1 cut(s)	3413
MluNI	TGGCCA	1 cut(s)	3413
Mly113I	GGCGCC	1 cut(s)	1657
MlyI	GAGTC	6 cut(s)	443, 499, 3332, 4249, 4369, 5179
MmeI	TCCRAC	5 cut(s)	1570, 1604, 1918, 4845, 5646
Mox20I	TGGCCA	1 cut(s)	3413
Mph1103I	ATGCAT	2 cut(s)	76, 4122
MroXI	GAANNNNTTC	7 cut(s)	1426, 2014, 2274, 4027, 4353, 4872, 5473
MscI	TGGCCA	1 cut(s)	3413
MslI	CAYNNNNRTG	3 cut(s)	3134, 4134, 4764
Msp20I	TGGCCA	1 cut(s)	3413
MspA1I	CMGCKG	3 cut(s)	538, 3065, 4087
MspI	CCGG	7 cut(s)	102, 3093, 3842, 4747, 4785, 4804, 5209
MspR9I	CCNGG	9 cut(s)	344, 458, 1339, 1745, 1922, 2301, 2642, 3813, 5648
MunI	CAATTG	3 cut(s)	404, 3861, 3894
Mva1269I	GAATGC	3 cut(s)	966, 1085, 1526
MvaI	CCWGG	9 cut(s)	344, 458, 1339, 1745, 1922, 2301, 2642, 3813, 5648
MvnI	CGCG	2 cut(s)	503, 822
NarI	GGCGCC	1 cut(s)	1657
NcoI	CCATGG	1 cut(s)	4759
NdeI	CATATG	3 cut(s)	76, 1104, 3592
NheI	GCTAGC	2 cut(s)	307, 2537
NmeAIII	GCCGAG	1 cut(s)	5611
NmuCI	GTSAC	4 cut(s)	953, 1308, 3320, 4252
NruI	TCGCGA	1 cut(s)	503
NsbI	TGCGCA	1 cut(s)	1847
NsiI	ATGCAT	2 cut(s)	76, 4122
NspI	RCATGY	6 cut(s)	766, 1951, 1984, 4013, 4408, 4811
PaeI	GCATGC	1 cut(s)	4408
PaeR7I	CTCGAG	3 cut(s)	1604, 3537, 4188
PciI	ACATGT	2 cut(s)	1980, 4009
PciSI	GCTCTTC	2 cut(s)	552, 3690
PcsI	WCGNNNNNNNCGW	1 cut(s)	3316
PctI	GAATGC	3 cut(s)	966, 1085, 1526
PdmI	GAANNNNTTC	7 cut(s)	1426, 2014, 2274, 4027, 4353, 4872, 5473
PfeI	GAWTC	9 cut(s)	198, 739, 1897, 2223, 2582, 3059, 3904, 4099, 5178
PflMI	CCANNNNNTGG	3 cut(s)	3085, 3409, 5463
PfoI	TCCNGGA	3 cut(s)	1743, 2299, 2640
PleI	GAGTC	6 cut(s)	442, 499, 3332, 4249, 4368, 5178
PluTI	GGCGCC	1 cut(s)	1660
PpsI	GAGTC	6 cut(s)	442, 499, 3332, 4249, 4368, 5178
PscI	ACATGT	2 cut(s)	1980, 4009
PshBI	ATTAAT	1 cut(s)	2813
PsiI	TTATAA	1 cut(s)	5282
Psp124BI	GAGCTC	2 cut(s)	564, 3571
Psp1406I	AACGTT	1 cut(s)	5621
Psp6I	CCWGG	9 cut(s)	342, 456, 1337, 1743, 1920, 2299, 2640, 3811, 5646
PspEI	GGTNACC	1 cut(s)	4543
PspFI	CCCAGC	2 cut(s)	5097, 5558
PspGI	CCWGG	9 cut(s)	342, 456, 1337, 1743, 1920, 2299, 2640, 3811, 5646
PspPI	GGNCC	8 cut(s)	65, 709, 1335, 1577, 2520, 2997, 4111, 4659
PspXI	VCTCGAGB	1 cut(s)	3537
PstI	CTGCAG	1 cut(s)	3108
PstNI	CAGNNNCTG	4 cut(s)	689, 2144, 4357, 5338
PsuI	RGATCY	5 cut(s)	181, 2182, 2878, 3004, 5410
PvuII	CAGCTG	2 cut(s)	538, 3065
RruI	TCGCGA	1 cut(s)	503
RseI	CAYNNNNRTG	3 cut(s)	3134, 4134, 4764
Rsr2I	CGGWCCG	1 cut(s)	1577
RsrII	CGGWCCG	1 cut(s)	1577
SacI	GAGCTC	2 cut(s)	564, 3571
SapI	GCTCTTC	2 cut(s)	552, 3690
Sau96I	GGNCC	8 cut(s)	65, 709, 1335, 1577, 2520, 2997, 4111, 4659
ScaI	AGTACT	2 cut(s)	701, 1301
SchI	GAGTC	6 cut(s)	443, 499, 3332, 4249, 4369, 5179
ScrFI	CCNGG	9 cut(s)	344, 458, 1339, 1745, 1922, 2301, 2642, 3813, 5648
SduI	GDGCHC	6 cut(s)	564, 1261, 2158, 3210, 3571, 4302
SfcI	CTRYAG	2 cut(s)	3104, 3211
SfoI	GGCGCC	1 cut(s)	1658
Sfr274I	CTCGAG	3 cut(s)	1604, 3537, 4188
SinI	GGWCC	2 cut(s)	1577, 4111
SlaI	CTCGAG	3 cut(s)	1604, 3537, 4188
SmiMI	CAYNNNNRTG	3 cut(s)	3134, 4134, 4764
SmlI	CTYRAG	6 cut(s)	6, 563, 1604, 3371, 3537, 4188
SmoI	CTYRAG	6 cut(s)	6, 563, 1604, 3371, 3537, 4188
SphI	GCATGC	1 cut(s)	4408
SspDI	GGCGCC	1 cut(s)	1656
SspI	AATATT	4 cut(s)	619, 1168, 1390, 2682
SstI	GAGCTC	2 cut(s)	564, 3571
StyD4I	CCNGG	9 cut(s)	342, 456, 1337, 1743, 1920, 2299, 2640, 3811, 5646
StyI	CCWWGG	7 cut(s)	663, 1376, 3000, 4243, 4719, 4759, 5139
TaiI	ACGT	8 cut(s)	1200, 2093, 2942, 3100, 4296, 4536, 5430, 5624
TaqII	GACCGA	1 cut(s)	1565
TatI	WGTACW	6 cut(s)	699, 1032, 1299, 1977, 4445, 5677
TauI	GCSGC	2 cut(s)	85, 3530
TfiI	GAWTC	9 cut(s)	198, 739, 1897, 2223, 2582, 3059, 3904, 4099, 5178
TseFI	GTSAC	4 cut(s)	953, 1308, 3320, 4252
Tsp45I	GTSAC	4 cut(s)	953, 1308, 3320, 4252
TspGWI	ACGGA	7 cut(s)	26, 2977, 3146, 3325, 3968, 4103, 5522
Van91I	CCANNNNNTGG	3 cut(s)	3085, 3409, 5463
VpaK11BI	GGWCC	2 cut(s)	1577, 4111
VspI	ATTAAT	1 cut(s)	2813
XbaI	TCTAGA	3 cut(s)	194, 235, 2612
XceI	RCATGY	6 cut(s)	766, 1951, 1984, 4013, 4408, 4811
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1269, 3968, 4957
XhoI	CTCGAG	3 cut(s)	1604, 3537, 4188
XmiI	GTMKAC	2 cut(s)	2093, 4371
XmnI	GAANNNNTTC	7 cut(s)	1426, 2014, 2274, 4027, 4353, 4872, 5473
ZrmI	AGTACT	2 cut(s)	701, 1301
Zsp2I	ATGCAT	2 cut(s)	76, 4122

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.