Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0011696

Physical Location & Seq

Genomic Coordinates Forward (+)

85872333 .. 85895118

Gene Structure 22786 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG556900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5718 bp

ATGGCTCAAGTTGAGGATCGATGGGAGCGGTTAGTCCGCGCAGTATTGAGGAGGGAAAGGATGGGGCCTGATGCATACGAGCGGCATGGGACTGGCATTGCCGGAAATGTTCCATCTTCACTTGAAAATAATAGGGATATTGATGAAATTCTCAGAGTTGCAGATGAAATCCAAGATGAAGATCCCAATATCTCTAGAATCCTATGTGAGCATGGTTATTCGTTGGCTCAAAATCTAGACCCCAATAGTGAAGGAAGGGGAGTATTACAGTTCAAGACTGGATTGATGTCTGTCATTAAGCAAAAGCTAGCGAAGAGGGAAGGGGCAACCATAGATAGAAGCCAAGACATTGTTCGCTTACAAGAGTTCTATAAACAGTATAGACAGAAAAATAATGTTGAACAATTGCGTGAGGAAGAAATGAAGTTGAGGGAGTCTGGTGTTTTCAGTGGAAACCTGGGCGAACTGGAAAGGAAAACAGTGAAGAGGAAAAGAGTATTCGCGACTCTAAGAGTTCTGGGCAAAGTTTTGGAGCAGCTGACAGAGGACATTCCAGAAGAGCTCAAGCACCAAATGGAGATAGATGCCGCAATGACTGAAGACCTGATTGCGTACAATATTATTCCACTGGATGCTCCTAGCATTACAAATGTTATCATGTCCTTGGCTGAGGTTCAAGCAGCAGTTTCTGGGTTAAAGTACTTCAGGGGCCTACCAAAGTTACCGACAGATTTTCCAATTCCTGCTACACGAGAGCCTGACATGCTTGATTTCCTGCATTATGTATTTGGATTTCAGAAAGACAACGTTTGTAACCAGCGCGAACATATTGTGCATCTCCTTGCAAATGAGCAATCACGGCTTCGTATTCCTGAAGAAACTGAACCTATATTGGATGAGGCTGCGGTGCAAAATATATTTTTAAAGTCCCTAGACAACTATATTAAGTGGTGTAACTACTTGTGCATTCAGCCAGTTTGGAGCAATTTGCAGTCAGTTAGCAAGGAGAAAAAGCTACTGTTTGTTTCCATGTACTGTTTGATCTGGGGTGAAGCAGCCAATGTTAGATTTCTTCCAGAATGCTTGTGCTACATATTTCATCATATGGCAAGGGAAATGGATGAAATTCTGAGGCAGCAGACTGCACAGCCAGCCAACAGTTGTAATTCTGAAAATGATGGAGTTTCTTTCCTTGATCAAGTTATATATCCTCTGTTTGAGATTGTGTCTGCGGAAGCTGGAAACAATGAAAATGGACGAGCACCACATTCTGCTTGGCGCAATTATGATGATTTTAATGAGTACTTCTGGTCACTCCATTGTTTTGAACTTAGTTGGCCCTGGCGCAAAGGTTCATCATTTTTTCAAAAGCCAACGCCAAGGTCAAAGAATATTCTCAAATCTGGCAGAAGTCAACATCGTGGAAAAACTTCATTTGTTGAGCATAGGACATTTCTCCATCTGTATCATAGTTTCCACCGTCTGTGGATATTCCTTGTGATGATGTTTCAGGGACTTGCGATCATTGCATTCAATGGAAGTTTTGATGCAAAATGTATTAGAGAAATTCTCAGTCTCGGTCCGACATTTGTTGGAATGAAATTTCTCGAGAGTGTTCTTGATGTTCTTATGATGTATGGCGCCTATTCTACAACACGGCGCCTAGCAGTTTCTCGAATTTTTCTCCGATTTGTTTGGTTTGGCACTGCATCAGTGGTCATATCTTTTCTTTATGTGAAAGCTCTCCAGGAAGAGAGTAAACAAAATGGAAACCCTATTATCTACAAGTTGTATCTGCTCATTGTAGGCATATATGCTGGTATCCAGTTCTTCATTAGTTTTTTCATGCGCATACCAGCCTGCCACAATCTGACTAATCAATGTGATCGCTGGCCTCTGATTCGCCTTGTCAAGTGGATGCGCCAGGAACGGTACTATGTTGGACGGGGCATGTATGAGAGGACTACTGATTTCATCAAGTACATGTTTTTCTGGCTTGTGATTTTGAGTGGAAAGTTTTCATTTGCATACTTTCTCCAGATCAAGCCGTTGGTGGAACCAACCAGAATTATAGTGAACACAAATGCTATAACGTATACTTGGCATGATTTTGTTTCTAAAAACAACTATAATGCTCTGACAGTTGCTGCTTTGTGGGCTCCCGTGATAATTATTTATCTTTTAGATCTCTATGTATTTTACACCGTTATATCTGCTGTGTGGGGATTCTTGCTTGGTGCCAGAGATCGTCTTGGAGAGATTAGATCATTGGAAGCACTTCATAAACTATTTGAGCAGTTTCCGGGAGCTTTTATGGACACTCTTCATATCCGCCTGCCTAACAGGGCTTACCATCAGTCTTCCAGTGAGGATATTGAGAAGAACAAGGTTGATGCTAGTCGATTTTCTCCCTTTTGGAATGAAATCATAAAAAATCTGAGGGAAGAAGATTATATCACTGACCTAGAAATGGAGCTGCTTGTAATGCCTAAAAATTCTGGAAGTCTTCCCTTAGTTCAGTGGCCCCTTTTCCTTCTTGCTAGCAAGATATTTGTTGCCAAAGATATTGCTCTTGAAACCAGGGATTCACAAGATGAGCTTTGGGAAAGAATTTCTAGAGATGACTATATGAAGTATGCTGTCCAGGAGTGCTTCCATAGCATTAAGCTTATCCTAAGTGAAATATTGGAGGGTGAAGGAAAGCTGTGGGTTGAAAGGATATATGATGATATTCAAGTAAGCATAGTGAAGAAGAGTATTCAGGCTGACTTTCAACTGAACAAGCTGCCACTCGTGATTTCAAGAGTTACTGCATTAATGGGAATTCTGAAAAAAGAGGAAACATCTGAACTGGTGGATGGTGCTATTAAGGCTGTTCAAGATCTCTACGACATTGTGCGCCATGATGTCCTTTCTATTAACATGAGGGAAAACTATGAAACATGGAATTTGTTGTCAAAAGCACGGACGGATGGTCGGTTGTTTGCCAAGTTGAAGTGGCCCAAGGATCCTTCATTGAGAGCACAAGTCAAAAGATTGTATTCATTGTTGACAATTCAAGATTCAGCTGCCAATGTCCCCCAAAATCTGGAGGCCGGACGTAGGCTGGAGTTCTTTACAAATTCTCTTTTCATGGAGATGCCCGAAGCAAGGCCAGTCCGTGAAATGTTGTCGTTCAGTGTTTTTACTCCATATTATGCTGAGACTGTGCTCTATAGTATCGCTGAACTCCAGAAGAAAAATGAAGATGGGATATCAGTATTGTTTTACCTTCAAAAGATTTACCCAGATGAGTGGAAGAACTTTCTTTTACGGATTGGCCATGACGAAAATGCACATGACTCGGAACTTTATGATAATCCCTCTGATATTCTTGAGCTTCGGTTTTGGGCGTCTTACCGAGGCCAAACATTGGCCAGAACTGTTCGTGGGATGATGTATTATAGGAAGGCTCTTATGCTTCAGACCTATTTGGAGAGATTGAATTCTGGAGTATCAGATGTGGAGGCTGCAATTTCTAGTGGTGATGCGGCTGAAACTCGAGCATTTGAGTTATCTCCTGAAGCACGAGCTCAGGCAGACCTGAAGTTTACATATGTAGTTACTTGTCAAATATATGGAAAACAGAAAGAAGGCCAAAAACCTGAGGCTGCTGATATAGCATTGCTTATGCAAAGAAATGAGGCTCTTCGAGTTGCTTTTATCGATGAGGTTGAGACTTTGAAGGATGGTAAAGTGAACAAAGAGTTCTATTCAAAACTTGTAAAGGCAGATATCAATGGGAAGGACAAGGAAATCTACTCTATAAAACTGCCTGGAAATCCAAAAATAGGTGAAGGGAAACCGGAGAACCAAAATCATGCAATTGTATTTACTCGTGGAAATGCAATTCAGACAATTGACATGAATCAGGATAACTATTTTGAGGAAGCTTTGAAGATGAGAAATTTACTCGAAGAATTCCACCGTGATCATGGTATCCGTCGTCCTACAATTCTTGGTGTCAGGGAACATGTCTTTACGGGAAGTGTTTCTTCTTTAGCGTCATTCATGTCCAATCAGGAAACAAGCTTTGTAACTCTAGCTCAGCGGGTTCTTGCCAATCCTTTGAAGGTCCGTATGCATTATGGTCATCCTGATGTGTTTGATAGAGTTTTCCACATAACACGGGGTGGTATTAGCAAGGCTTCTCGAGTGATTAACATCAGTGAAGACATCTTTGCAGGCTTCAACTCAACTTTACGCCAAGGAAATGTGACTCACCATGAGTATATTCAGGTTGGCAAAGGGCGGGACGTTGGGCTCAACCAAATTGCAGTATTTGAGGGGAAGGTTGCTGGTGGTAATGGTGAACAGGTTCTGAGTCGTGATGTATACAGGCTTGGGCAGCAGTTTGATTTCTTCCGCATGCTGTCATTTTACTTTACCACTGTCGGCTACTACTTCTGTACAATGTTAACAGTGCTGACGGTTTACATTTTTCTCTACGGGAAAACATATATGGCGCTATCTGGGGTTGGTGAATACTTGCAGAACGTAGCTGAGGTAACCAAGAATACCGCACTGACTGCTGCTCTAAATACACAATTCCTCCTTCAAATTGGTATCTTTACTGCTGTCCCGATGATTTTAGGCTCCATCTTGGAACAGGGTTTCCTGCGGGCCATAGTAAGTTTCCTCACAATGCAGTTGCAACTTTGTTCTGTCTTCTTCACATTCTCCTTGGGAACCAAAACACATTATTTTGGCCGGACAATTCTCCATGGTGGTGCAAAGTATCAAGCAACCGGCAGGGGATTTGTAGTCCGGCATGTTAAGTTTACAGAGAATTACAGGCTTTACTCCCGCAGTCATTTTACCAAAGGGTTGGAAGTAGTTCTTTTGTTGGTAGTGTACCTTGCTTATGGATATGATGATGGCGGTGCTATATCATACATTCTTCTTTCAATAACCAGTTGGTTCATGGCTCTTTCTTGGCTTTTTGCTCCCTATTTGTTCAACCCATCTGGGTTTGAGTGGCAGAAGGTTGTGGAGGATTTCCGAGATTGGACAAATTGGCTTCTTTATAGAGGTGGAATTGGAGTTAAGGGTGAAGAAAGCTGGGAAGCTTGGTGGGAGGAAGAACTGGCGCATATTAGAACCTTGGGAGGAAGGATTGCAGAGACAATTTTGAGTCTAAGATTCTTTATCTTCCAGTATGGAATTATCTACCGGCTAGATGTGAAGGGATCAGATACATCACTAACGGTGTATGGCCTCTCATGGATCGTGCTGGCGGTGCTTATAATTCTTTTCAAGGTTTTCACCTTCAGCCAGAAGATATCTGTCAACTTCCAGCTTCTGTTGCGCTTCATTCAAGGGGTTGCCTTCATGTTGGCACTGGCTGGTTTAGCAGTAGCAGTCAAATTTACAGATCTGACAATCGCAGACGTTTTTGCTTCCATTTTAGCGTTTGTACCCACTGGGTGGGGAATTCTTTCCATTTGCATAGCTTGGAAACCCTTGATGAAGAAACTGGGCTTGTGGAAATCCATCCGTTCCATTGCCCTTCTTTATGATGCTGGGATGGGTATGGTTATATTCATTCCCATTGCATTGTCATCATGGTTCCCATTTGTATCAACGTTTCAAACTCGGCTCATGTTCAACCAGGCTTTCAGTCGAGGGTTGGAAATCTCTGTGCTACTTGCTGGAAACAACCCCAATTCTGGATTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000278 GO:0000280 GO:0001558 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0006996 GO:0007049 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009556 GO:0009846 GO:0009856 GO:0009987 GO:0010769 GO:0012505 GO:0016020 GO:0016043 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022603 GO:0022604 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0033037 GO:0034293 GO:0040007 GO:0040008 GO:0042545 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0043934 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044706 GO:0045229 GO:0045595 GO:0048229 GO:0048232 GO:0048236 GO:0048285 GO:0048589 GO:0048609 GO:0048638 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051179 GO:0051239 GO:0051321 GO:0051510 GO:0051641 GO:0051704 GO:0052386 GO:0052543 GO:0052545 GO:0055047 GO:0060284 GO:0065007 GO:0070727 GO:0071554 GO:0071555 GO:0071840 GO:0071944 GO:0080092 GO:0140014 GO:1903046 GO:1903047 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase

Protein Analysis

1905

Amino Acids

219.03

Weight (kDa)

7.57

Isoelectric Point (pI)

36.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FKS1_dom1	PF14288	338 - 446	5.6e-40	1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1	PF25968	842 - 1019	6e-48	Callose synthase 1 domain
Glucan_synthase	PF02364	1026 - 1716	1.5e-232	1,3-beta-glucan synthase component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011696)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07160 AT3G07160 AT3G07160
fragaria_vesca	FvH4_3g44320
malus_domestica	MD11G1016100.v1.1
prunus_persica	Prupe.6G014200_v2.0.a1 Prupe.6G014200_v2.0.a1
pyrus_communis	pycom11g01130
rosa_chinensis	RchiOBHm_Chr5g0080681
rosa_laevigata	RLG00000036885
rosa_roxburghii	Rroxscaffold_1G00002730
rosa_rugosa	Rorug05G0471400 Rorug05G0471400 Rorug05G0471400
rosa_samantha	Rh5AG524500 Rh5BG546700 Rh5CG570000 Rh5DG556900
rosa_wichuraiana	Rw5G048650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	5285
Acc16I	TGCGCA	1 cut(s)	1850
AccB1I	GGYRCC	3 cut(s)	1640, 1659, 2237
AccB7I	CCANNNNNTGG	3 cut(s)	3088, 3412, 5466
AccBSI	CCGCTC	2 cut(s)	28, 82
AccI	GTMKAC	2 cut(s)	2096, 4374
AccII	CGCG	3 cut(s)	39, 503, 822
AclI	AACGTT	2 cut(s)	807, 5624
AclWI	GGATC	6 cut(s)	24, 176, 3002, 3015, 5236, 5273
AcoI	YGGCCR	3 cut(s)	3319, 3414, 4747
AcuI	CTGAAG	7 cut(s)	618, 688, 894, 3446, 3582, 3605, 5293
AcyI	GRCGYC	3 cut(s)	1641, 1660, 3392
AdeI	CACNNNGTG	2 cut(s)	4172, 5466
AfaI	GTAC	9 cut(s)	614, 701, 1034, 1304, 1934, 1982, 4450, 4895, 5457
AfiI	CCNNNNNNNGG	8 cut(s)	2470, 3088, 3102, 3150, 3412, 3830, 5466, 5708
AflIII	ACRYGT	2 cut(s)	1983, 4012
AhdI	GACNNNNNGTC	1 cut(s)	2973
AjnI	CCWGG	8 cut(s)	456, 1340, 1746, 1923, 2579, 2643, 3814, 5649
AjuI	GAANNNNNNNTTGG	2 cut(s)	336, 368
AloI	GAACNNNNNNTCC	2 cut(s)	4638, 4670
Alw21I	GWGCWC	5 cut(s)	564, 1264, 3025, 3213, 3574
Alw26I	GTCTC	4 cut(s)	1580, 3197, 3710, 5156
AlwI	GGATC	6 cut(s)	24, 176, 3002, 3015, 5236, 5273
AlwNI	CAGNNNCTG	4 cut(s)	689, 2147, 4360, 5341
Ama87I	CYCGRG	3 cut(s)	1607, 3540, 4191
AseI	ATTAAT	1 cut(s)	2816
Asp700I	GAANNNNTTC	7 cut(s)	1429, 2017, 2277, 4030, 4356, 4875, 5476
AspS9I	GGNCC	8 cut(s)	65, 709, 1338, 1580, 2523, 3000, 4114, 4662
AsuC2I	CCSGG	1 cut(s)	2304
AsuHPI	GGTGA	9 cut(s)	1061, 2705, 3536, 3845, 4253, 4361, 4532, 5102, 5296
AsuNHI	GCTAGC	2 cut(s)	307, 2540
AvaI	CYCGRG	3 cut(s)	1607, 3540, 4191
AvaII	GGWCC	2 cut(s)	1580, 4114
AxyI	CCTNAGG	1 cut(s)	3645
BalI	TGGCCA	2 cut(s)	3321, 3416
BamHI	GGATCC	1 cut(s)	3007
BanI	GGYRCC	3 cut(s)	1640, 1659, 2237
BanII	GRGCYC	4 cut(s)	564, 2161, 3574, 4305
BauI	CACGAG	4 cut(s)	750, 2792, 3567, 3876
BbsI	GAAGAC	5 cut(s)	606, 2352, 2498, 4218, 4699
Bbv12I	GWGCWC	5 cut(s)	564, 1264, 3025, 3213, 3574
BbvCI	CCTCAGC	2 cut(s)	669, 4542
BceAI	ACGGC	3 cut(s)	875, 1673, 2032
BcgI	CGANNNNNNTGC	2 cut(s)	3324, 3358
BciT130I	CCWGG	8 cut(s)	458, 1342, 1748, 1925, 2581, 2645, 3816, 5651
BciVI	GTATCC	2 cut(s)	1832, 3989
BclI	TGATCA	2 cut(s)	1195, 3970
BcnI	CCSGG	1 cut(s)	2304
BcoDI	GTCTC	4 cut(s)	1580, 3197, 3710, 5156
BfmI	CTRYAG	1 cut(s)	3214
BfoI	RGCGCY	3 cut(s)	1644, 1663, 4508
BfuI	GTATCC	2 cut(s)	1832, 3989
BglII	AGATCT	3 cut(s)	2185, 2881, 5413
BlpI	GCTNAGC	1 cut(s)	4086
BmcAI	AGTACT	2 cut(s)	701, 1304
Bme1390I	CCNGG	9 cut(s)	458, 1342, 1748, 1925, 2304, 2581, 2645, 3816, 5651
Bme18I	GGWCC	2 cut(s)	1580, 4114
BmeRI	GACNNNNNGTC	1 cut(s)	2973
BmeT110I	CYCGRG	3 cut(s)	1607, 3540, 4191
BmgT120I	GGNCC	8 cut(s)	65, 709, 1338, 1580, 2523, 3000, 4114, 4662
BmrFI	CCNGG	9 cut(s)	458, 1342, 1748, 1925, 2304, 2581, 2645, 3816, 5651
BmrI	ACTGGG	2 cut(s)	5472, 5525
BmtI	GCTAGC	2 cut(s)	311, 2544
BmuI	ACTGGG	2 cut(s)	5472, 5525
BpiI	GAAGAC	5 cut(s)	606, 2352, 2498, 4218, 4699
BplI	GAGNNNNNCTC	2 cut(s)	1554, 1586
BpmI	CTGGAG	6 cut(s)	1730, 2021, 3110, 3128, 3215, 3510
Bpu10I	CCTNAGC	3 cut(s)	669, 3573, 4542
Bpu1102I	GCTNAGC	1 cut(s)	4086
BpuEI	CTTGAG	2 cut(s)	548, 3395
BpuMI	CCSGG	1 cut(s)	2304
Bsa29I	ATCGAT	2 cut(s)	19, 3705
BsaBI	GATNNNNATC	2 cut(s)	180, 4202
BsaHI	GRCGYC	3 cut(s)	1641, 1660, 3392
BsaWI	WCCGGW	1 cut(s)	3844
BsaXI	ACNNNNNCTCC	2 cut(s)	4575, 4605
Bsc4I	CCNNNNNNNGG	8 cut(s)	2470, 3088, 3102, 3150, 3412, 3830, 5466, 5708
Bse118I	RCCGGY	2 cut(s)	4787, 5211
Bse21I	CCTNAGG	1 cut(s)	3645
Bse3DI	GCAATG	6 cut(s)	96, 597, 1524, 3662, 5541, 5589
Bse8I	GATNNNNATC	2 cut(s)	180, 4202
BseBI	CCWGG	8 cut(s)	458, 1342, 1748, 1925, 2581, 2645, 3816, 5651
BseCI	ATCGAT	2 cut(s)	19, 3705
BseJI	GATNNNNATC	2 cut(s)	180, 4202
BseLI	CCNNNNNNNGG	8 cut(s)	2470, 3088, 3102, 3150, 3412, 3830, 5466, 5708
BseMI	GCAATG	6 cut(s)	96, 597, 1524, 3662, 5541, 5589
BseRI	GAGGAG	1 cut(s)	64
BseYI	CCCAGC	2 cut(s)	5100, 5561
BsgI	GTGCAG	1 cut(s)	1128
Bsh1236I	CGCG	3 cut(s)	39, 503, 822
BshNI	GGYRCC	3 cut(s)	1640, 1659, 2237
BshVI	ATCGAT	2 cut(s)	19, 3705
BsiHKAI	GWGCWC	5 cut(s)	564, 1264, 3025, 3213, 3574
BsiHKCI	CYCGRG	3 cut(s)	1607, 3540, 4191
BsiSI	CCGG	8 cut(s)	102, 2303, 3096, 3845, 4750, 4788, 4807, 5212
BslFI	GGGAC	6 cut(s)	103, 913, 1527, 3062, 4307, 4604
BslI	CCNNNNNNNGG	8 cut(s)	2470, 3088, 3102, 3150, 3412, 3830, 5466, 5708
BsmAI	GTCTC	4 cut(s)	1580, 3197, 3710, 5156
BsmFI	GGGAC	6 cut(s)	103, 913, 1527, 3062, 4307, 4604
BsmI	GAATGC	3 cut(s)	966, 1085, 1529
BsoBI	CYCGRG	3 cut(s)	1607, 3540, 4191
Bsp1286I	GDGCHC	7 cut(s)	564, 1264, 2161, 3025, 3213, 3574, 4305
Bsp1407I	TGTACA	1 cut(s)	4448
Bsp1720I	GCTNAGC	1 cut(s)	4086
Bsp19I	CCATGG	1 cut(s)	4762
Bsp68I	TCGCGA	1 cut(s)	503
BspDI	ATCGAT	2 cut(s)	19, 3705
BspFNI	CGCG	3 cut(s)	39, 503, 822
BspOI	GCTAGC	2 cut(s)	311, 2544
BspPI	GGATC	6 cut(s)	24, 176, 3002, 3015, 5236, 5273
BspQI	GCTCTTC	2 cut(s)	552, 3693
BspT107I	GGYRCC	3 cut(s)	1640, 1659, 2237
BsrBI	CCGCTC	2 cut(s)	28, 82
BsrDI	GCAATG	6 cut(s)	96, 597, 1524, 3662, 5541, 5589
BsrFI	RCCGGY	2 cut(s)	4787, 5211
BsrGI	TGTACA	1 cut(s)	4448
BssAI	RCCGGY	2 cut(s)	4787, 5211
BssNAI	GTATAC	2 cut(s)	2097, 4375
BssNI	GRCGYC	3 cut(s)	1641, 1660, 3392
BssSI	CACGAG	4 cut(s)	750, 2792, 3567, 3876
BssT1I	CCWWGG	7 cut(s)	663, 1379, 3003, 4246, 4722, 4762, 5142
Bst1107I	GTATAC	2 cut(s)	2097, 4375
Bst2BI	CACGAG	4 cut(s)	750, 2792, 3567, 3876
Bst2UI	CCWGG	8 cut(s)	458, 1342, 1748, 1925, 2581, 2645, 3816, 5651
Bst6I	CTCTTC	7 cut(s)	308, 479, 552, 1746, 2328, 2747, 3693
BstACI	GRCGYC	3 cut(s)	1641, 1660, 3392
BstAPI	GCANNNNNTGC	1 cut(s)	4568
BstAUI	TGTACA	1 cut(s)	4448
BstDSI	CCRYGG	1 cut(s)	4762
BstEII	GGTNACC	1 cut(s)	4546
BstFNI	CGCG	3 cut(s)	39, 503, 822
BstH2I	RGCGCY	3 cut(s)	1644, 1663, 4508
BstMAI	GTCTC	4 cut(s)	1580, 3197, 3710, 5156
BstNI	CCWGG	8 cut(s)	458, 1342, 1748, 1925, 2581, 2645, 3816, 5651
BstNSI	RCATGY	6 cut(s)	766, 1954, 1987, 4016, 4411, 4814
BstPI	GGTNACC	1 cut(s)	4546
BstSCI	CCNGG	9 cut(s)	456, 1340, 1746, 1923, 2302, 2579, 2643, 3814, 5649
BstSFI	CTRYAG	1 cut(s)	3214
BstUI	CGCG	3 cut(s)	39, 503, 822
BstV2I	GAAGAC	5 cut(s)	606, 2352, 2498, 4218, 4699
BstX2I	RGATCY	5 cut(s)	181, 2185, 2881, 3007, 5413
BstXI	CCANNNNNNTGG	2 cut(s)	3294, 4867
BstYI	RGATCY	5 cut(s)	181, 2185, 2881, 3007, 5413
BstZ17I	GTATAC	2 cut(s)	2097, 4375
Bsu15I	ATCGAT	2 cut(s)	19, 3705
Bsu36I	CCTNAGG	1 cut(s)	3645
BsuI	GTATCC	2 cut(s)	1832, 3989
BsuTUI	ATCGAT	2 cut(s)	19, 3705
BtgI	CCRYGG	1 cut(s)	4762
BtsI	GCAGTG	1 cut(s)	1704
BtuMI	TCGCGA	1 cut(s)	503
CaiI	CAGNNNCTG	4 cut(s)	689, 2147, 4360, 5341
Cfr10I	RCCGGY	2 cut(s)	4787, 5211
Cfr13I	GGNCC	8 cut(s)	65, 709, 1338, 1580, 2523, 3000, 4114, 4662
ClaI	ATCGAT	2 cut(s)	19, 3705
CpoI	CGGWCCG	1 cut(s)	1580
CseI	GACGC	2 cut(s)	3381, 4032
Csp6I	GTAC	9 cut(s)	613, 700, 1033, 1303, 1933, 1981, 4449, 4894, 5456
CspI	CGGWCCG	1 cut(s)	1580
CviQI	GTAC	9 cut(s)	613, 700, 1033, 1303, 1933, 1981, 4449, 4894, 5456
DinI	GGCGCC	2 cut(s)	1642, 1661
DraI	TTTAAA	1 cut(s)	924
DraIII	CACNNNGTG	2 cut(s)	4172, 5466
DriI	GACNNNNNGTC	1 cut(s)	2973
EaeI	YGGCCR	3 cut(s)	3319, 3414, 4747
Eam1104I	CTCTTC	7 cut(s)	308, 479, 552, 1746, 2328, 2747, 3693
Eam1105I	GACNNNNNGTC	1 cut(s)	2973
EarI	CTCTTC	7 cut(s)	308, 479, 552, 1746, 2328, 2747, 3693
EciI	GGCGGA	1 cut(s)	2321
Ecl136II	GAGCTC	2 cut(s)	562, 3572
Eco130I	CCWWGG	7 cut(s)	663, 1379, 3003, 4246, 4722, 4762, 5142
Eco24I	GRGCYC	4 cut(s)	564, 2161, 3574, 4305
Eco32I	GATATC	3 cut(s)	3255, 3775, 5322
Eco47I	GGWCC	2 cut(s)	1580, 4114
Eco53kI	GAGCTC	2 cut(s)	562, 3572
Eco57I	CTGAAG	7 cut(s)	618, 688, 894, 3446, 3582, 3605, 5293
Eco81I	CCTNAGG	1 cut(s)	3645
Eco88I	CYCGRG	3 cut(s)	1607, 3540, 4191
Eco91I	GGTNACC	1 cut(s)	4546
EcoICRI	GAGCTC	2 cut(s)	562, 3572
EcoO109I	RGGNCCY	2 cut(s)	65, 709
EcoO65I	GGTNACC	1 cut(s)	4546
EcoRI	GAATTC	4 cut(s)	2823, 3484, 3959, 5472
EcoRII	CCWGG	8 cut(s)	456, 1340, 1746, 1923, 2579, 2643, 3814, 5649
EcoRV	GATATC	3 cut(s)	3255, 3775, 5322
EcoT14I	CCWWGG	7 cut(s)	663, 1379, 3003, 4246, 4722, 4762, 5142
EcoT22I	ATGCAT	2 cut(s)	76, 4125
EcoT38I	GRGCYC	4 cut(s)	564, 2161, 3574, 4305
EgeI	GGCGCC	2 cut(s)	1642, 1661
EheI	GGCGCC	2 cut(s)	1642, 1661
ErhI	CCWWGG	7 cut(s)	663, 1379, 3003, 4246, 4722, 4762, 5142
FalI	AAGNNNNNCTT	6 cut(s)	2583, 2615, 4018, 4050, 4863, 4895
FaqI	GGGAC	6 cut(s)	103, 913, 1527, 3062, 4307, 4604
FauI	CCCGC	4 cut(s)	4083, 4284, 4653, 4853
FauNDI	CATATG	2 cut(s)	1104, 3595
FbaI	TGATCA	2 cut(s)	1195, 3970
FblI	GTMKAC	2 cut(s)	2096, 4374
FriOI	GRGCYC	4 cut(s)	564, 2161, 3574, 4305
FspAI	RTGCGCAY	1 cut(s)	1850
FspI	TGCGCA	1 cut(s)	1850
GsaI	CCCAGC	2 cut(s)	5104, 5565
GsuI	CTGGAG	6 cut(s)	1730, 2021, 3110, 3128, 3215, 3510
HaeII	RGCGCY	3 cut(s)	1644, 1663, 4508
HapII	CCGG	8 cut(s)	102, 2303, 3096, 3845, 4750, 4788, 4807, 5212
HgaI	GACGC	2 cut(s)	3381, 4032
Hin1I	GRCGYC	3 cut(s)	1641, 1660, 3392
HincII	GTYRAC	4 cut(s)	1415, 3051, 4458, 5329
HindII	GTYRAC	4 cut(s)	1415, 3051, 4458, 5329
HindIII	AAGCTT	4 cut(s)	2666, 3930, 4069, 5106
HpaI	GTTAAC	1 cut(s)	4458
HpaII	CCGG	8 cut(s)	102, 2303, 3096, 3845, 4750, 4788, 4807, 5212
HphI	GGTGA	9 cut(s)	1061, 2705, 3536, 3845, 4253, 4361, 4532, 5102, 5296
Hpy99I	CGWCG	1 cut(s)	3987
HpyCH4IV	ACGT	7 cut(s)	807, 2093, 3100, 4296, 4536, 5430, 5624
HpySE526I	ACGT	7 cut(s)	807, 2093, 3100, 4296, 4536, 5430, 5624
Hsp92I	GRCGYC	3 cut(s)	1641, 1660, 3392
KasI	GGCGCC	2 cut(s)	1640, 1659
Ksp22I	TGATCA	2 cut(s)	1195, 3970
KspAI	GTTAAC	1 cut(s)	4458
LguI	GCTCTTC	2 cut(s)	552, 3693
LmnI	GCTCC	9 cut(s)	25, 532, 640, 981, 2164, 2306, 2473, 4640, 4990
MaeII	ACGT	7 cut(s)	807, 2093, 3100, 4296, 4536, 5430, 5624
MaeIII	GTNAC	9 cut(s)	720, 812, 953, 1311, 2806, 3601, 4075, 4255, 4546
MbiI	CCGCTC	2 cut(s)	28, 82
MfeI	CAATTG	3 cut(s)	404, 3864, 3897
MflI	RGATCY	5 cut(s)	181, 2185, 2881, 3007, 5413
MhlI	GDGCHC	7 cut(s)	564, 1264, 2161, 3025, 3213, 3574, 4305
MlsI	TGGCCA	2 cut(s)	3321, 3416
MluNI	TGGCCA	2 cut(s)	3321, 3416
Mly113I	GGCGCC	2 cut(s)	1641, 1660
MlyI	GAGTC	6 cut(s)	443, 499, 3335, 4252, 4372, 5182
MmeI	TCCRAC	5 cut(s)	1573, 1607, 1921, 4848, 5649
Mox20I	TGGCCA	2 cut(s)	3321, 3416
Mph1103I	ATGCAT	2 cut(s)	76, 4125
MroXI	GAANNNNTTC	7 cut(s)	1429, 2017, 2277, 4030, 4356, 4875, 5476
MscI	TGGCCA	2 cut(s)	3321, 3416
MslI	CAYNNNNRTG	3 cut(s)	3137, 4137, 4767
Msp20I	TGGCCA	2 cut(s)	3321, 3416
MspA1I	CMGCKG	3 cut(s)	538, 3068, 4090
MspI	CCGG	8 cut(s)	102, 2303, 3096, 3845, 4750, 4788, 4807, 5212
MspR9I	CCNGG	9 cut(s)	458, 1342, 1748, 1925, 2304, 2581, 2645, 3816, 5651
MunI	CAATTG	3 cut(s)	404, 3864, 3897
Mva1269I	GAATGC	3 cut(s)	966, 1085, 1529
MvaI	CCWGG	8 cut(s)	458, 1342, 1748, 1925, 2581, 2645, 3816, 5651
MvnI	CGCG	3 cut(s)	39, 503, 822
NarI	GGCGCC	2 cut(s)	1641, 1660
NciI	CCSGG	1 cut(s)	2304
NcoI	CCATGG	1 cut(s)	4762
NdeI	CATATG	2 cut(s)	1104, 3595
NheI	GCTAGC	2 cut(s)	307, 2540
NmeAIII	GCCGAG	1 cut(s)	5614
NmuCI	GTSAC	2 cut(s)	1311, 4255
NruI	TCGCGA	1 cut(s)	503
NsbI	TGCGCA	1 cut(s)	1850
NsiI	ATGCAT	2 cut(s)	76, 4125
NspI	RCATGY	6 cut(s)	766, 1954, 1987, 4016, 4411, 4814
PaeI	GCATGC	1 cut(s)	4411
PaeR7I	CTCGAG	3 cut(s)	1607, 3540, 4191
PciI	ACATGT	2 cut(s)	1983, 4012
PciSI	GCTCTTC	2 cut(s)	552, 3693
PctI	GAATGC	3 cut(s)	966, 1085, 1529
PdmI	GAANNNNTTC	7 cut(s)	1429, 2017, 2277, 4030, 4356, 4875, 5476
PfeI	GAWTC	7 cut(s)	198, 1900, 2226, 2585, 3062, 3907, 5181
PflMI	CCANNNNNTGG	3 cut(s)	3088, 3412, 5466
PfoI	TCCNGGA	3 cut(s)	1746, 2302, 2643
PleI	GAGTC	6 cut(s)	442, 499, 3335, 4252, 4371, 5181
PluTI	GGCGCC	2 cut(s)	1644, 1663
PpsI	GAGTC	6 cut(s)	442, 499, 3335, 4252, 4371, 5181
PscI	ACATGT	2 cut(s)	1983, 4012
PshBI	ATTAAT	1 cut(s)	2816
PsiI	TTATAA	1 cut(s)	5285
Psp124BI	GAGCTC	2 cut(s)	564, 3574
Psp1406I	AACGTT	2 cut(s)	807, 5624
Psp6I	CCWGG	8 cut(s)	456, 1340, 1746, 1923, 2579, 2643, 3814, 5649
PspEI	GGTNACC	1 cut(s)	4546
PspFI	CCCAGC	2 cut(s)	5100, 5561
PspGI	CCWGG	8 cut(s)	456, 1340, 1746, 1923, 2579, 2643, 3814, 5649
PspPI	GGNCC	8 cut(s)	65, 709, 1338, 1580, 2523, 3000, 4114, 4662
PspXI	VCTCGAGB	1 cut(s)	3540
PstNI	CAGNNNCTG	4 cut(s)	689, 2147, 4360, 5341
PsuI	RGATCY	5 cut(s)	181, 2185, 2881, 3007, 5413
PvuII	CAGCTG	2 cut(s)	538, 3068
RruI	TCGCGA	1 cut(s)	503
RsaI	GTAC	9 cut(s)	614, 701, 1034, 1304, 1934, 1982, 4450, 4895, 5457
RsaNI	GTAC	9 cut(s)	613, 700, 1033, 1303, 1933, 1981, 4449, 4894, 5456
RseI	CAYNNNNRTG	3 cut(s)	3137, 4137, 4767
Rsr2I	CGGWCCG	1 cut(s)	1580
RsrII	CGGWCCG	1 cut(s)	1580
SacI	GAGCTC	2 cut(s)	564, 3574
SapI	GCTCTTC	2 cut(s)	552, 3693
Sau96I	GGNCC	8 cut(s)	65, 709, 1338, 1580, 2523, 3000, 4114, 4662
ScaI	AGTACT	2 cut(s)	701, 1304
SchI	GAGTC	6 cut(s)	443, 499, 3335, 4252, 4372, 5182
ScrFI	CCNGG	9 cut(s)	458, 1342, 1748, 1925, 2304, 2581, 2645, 3816, 5651
SduI	GDGCHC	7 cut(s)	564, 1264, 2161, 3025, 3213, 3574, 4305
SfcI	CTRYAG	1 cut(s)	3214
SfoI	GGCGCC	2 cut(s)	1642, 1661
Sfr274I	CTCGAG	3 cut(s)	1607, 3540, 4191
SinI	GGWCC	2 cut(s)	1580, 4114
SlaI	CTCGAG	3 cut(s)	1607, 3540, 4191
SmiMI	CAYNNNNRTG	3 cut(s)	3137, 4137, 4767
SmlI	CTYRAG	6 cut(s)	6, 563, 1607, 3374, 3540, 4191
SmoI	CTYRAG	6 cut(s)	6, 563, 1607, 3374, 3540, 4191
SphI	GCATGC	1 cut(s)	4411
SspDI	GGCGCC	2 cut(s)	1640, 1659
SspI	AATATT	3 cut(s)	619, 1393, 2685
SstI	GAGCTC	2 cut(s)	564, 3574
StyD4I	CCNGG	9 cut(s)	456, 1340, 1746, 1923, 2302, 2579, 2643, 3814, 5649
StyI	CCWWGG	7 cut(s)	663, 1379, 3003, 4246, 4722, 4762, 5142
TaiI	ACGT	7 cut(s)	810, 2096, 3103, 4299, 4539, 5433, 5627
TaqII	GACCGA	1 cut(s)	1568
TatI	WGTACW	5 cut(s)	699, 1032, 1302, 1980, 4448
TauI	GCSGC	3 cut(s)	85, 590, 3533
TfiI	GAWTC	7 cut(s)	198, 1900, 2226, 2585, 3062, 3907, 5181
TseFI	GTSAC	2 cut(s)	1311, 4255
Tsp45I	GTSAC	2 cut(s)	1311, 4255
TspGWI	ACGGA	7 cut(s)	2980, 2984, 3149, 3328, 3971, 4106, 5525
Van91I	CCANNNNNTGG	3 cut(s)	3088, 3412, 5466
VpaK11BI	GGWCC	2 cut(s)	1580, 4114
VspI	ATTAAT	1 cut(s)	2816
XbaI	TCTAGA	3 cut(s)	194, 235, 2615
XceI	RCATGY	6 cut(s)	766, 1954, 1987, 4016, 4411, 4814
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1272, 3971, 4960
XhoI	CTCGAG	3 cut(s)	1607, 3540, 4191
XmiI	GTMKAC	2 cut(s)	2096, 4374
XmnI	GAANNNNTTC	7 cut(s)	1429, 2017, 2277, 4030, 4356, 4875, 5476
ZrmI	AGTACT	2 cut(s)	701, 1304
Zsp2I	ATGCAT	2 cut(s)	76, 4125

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.