MD11G1055400.v1.1

Plant protein of unknown function (DUF641)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0011946
Physical Location & Seq
Reverse (-)
4705991 .. 4707772
1782 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1055400.v1.1.491

Sequence Viewer

Length: 1326 bp
ATGGAATTTGCTACTGCCAAACCCCTTAAACCCTCCTCAAACATATCTGATATTGTCTCCAAGTTTGCCAAAGTTTGCAAGTTGAGATCCATTGGCGTGTTTTCGTCCGAAAACCCGGATCATAACCATCAACCCAACAACCCCAACATTAGCAATGCACATTTGGGTGAGGATAGCAGCGATGTGACAGAGGAGACGGAGTGTGATGGGGTGAAGATCCACCCCCAACCCGTGGAAGTAAATAGAGCAAGTGATAAGTGTGGGGATGCAGAGTTATCCAAGTTGTTTGACATTGTTTCAGCTCTGAAATTAGCTTACGTTCAGCTTCAGCAAGCTCATTTGCCCTATAACCCGAAGAAGATTGTAGCTGCGGACGAACAATTTATGACTGAGCTTGAAGCGCTCTGCAAGATGAAGCGCGCACATAAGGAGAAGCAATGTATAAAGTTGAAGTCTGATCCCTCTCGTTCAGATATCTTGAAGAAGAAAGTTGAACTGAATGAGAAGCTACTGGACGAGTTGAAGTTTCAAATGGAAGTTAAGAACTCTGATATACTCTGCTTGCAAAAAGAGCTCAGGGATTTGGATTTGGTGAATGTGGCACTGGCTGAAAAGGTAAGGCAGGTAAGTTTGCAGAGGAAGAATGTAAGGGTGTGGAACGTTGCGACATTTCAGGATGCTTTCAGAGCTGCGTCGAAATCTATTCATGATTTTGCAAAGCCGTTAATTAGCTTGATGAAAGCTTCAGGCTGGGATTTGGATCTTGCGGCAAAGACAGTGGAAGTGGAAGCTGTGTATTCAAAAAGGGCCCACAAAAAATATGCATTTGAAGCCTACATTGCTAGGAGAATGTTTTATGGAATGTCATTGATATCTCATAATGTGGATGGTATTATGAGATATGATGACCCGATCGATGCCCTGATGGAGAACCCAGATTCCGATTTTGCAAGATTCTGTGGAGAAAAGTATCTCTTGGTTGTTCATCCGACGATGGAAGCCAAGTTTTTTGGGCATCTGGATCACAGGACTCTTGTATTGAGTGGCAAGCATCCGAGGACTTCATTCTACCAAATATTCGCGAGAATGGCAAGGTCGGTTTGGGTGTTACGTGGCACTGCACCTTTGATAGATTCTGAAGCAAAAATATTCGCTGTCGAAAGAGGAAGTAAATTCTCGGATGTCCATATGGAGTCCGTGGAGGAAGACGGGGAAGGCGCAGCTGTCTGGGGTGAACGACTGGTGGGGTTTATGGTCGTGCCCGGATTTAGAATTGGAGAGACAATTGTGAGGTCTCGGGTGTATGTTTCGAAATCAAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF641

Protein Analysis

442

Amino Acids

49.87

Weight (kDa)

8.29

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF641 PF04859 87 - 204 2.4e-25 Plant protein of unknown function (DUF641)
GIL1_IRKI_C PF24994 383 - 435 8.4e-19 GIL1/IRKI C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011946)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30380
fragaria_vesca FvH4_3g40290
malus_domestica MD03G1053800.v1.1 MD11G1055400.v1.1
prunus_persica Prupe.6G042900_v2.0.a1 Prupe.6G042900_v2.0.a1 Prupe.6G042900_v2.0.a1 Prupe.6G042900_v2.0.a1
pyrus_communis pycom03g04270
rosa_chinensis RchiOBHm_Chr5g0072631
rosa_laevigata RLG00000036318
rosa_multiflora Rmu_sc0002088.1_g000004
rosa_roxburghii Rroxscaffold_1G00008520
rosa_rugosa Rorug05G0418900
rosa_samantha Rh5AG475800 Rh5BG495900 Rh5CG519700 Rh5DG508400
rosa_wichuraiana Rw5G044180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 613
AccB7I CCANNNNNTGG 1 cut(s) 232
AccII CGCG 2 cut(s) 420, 1082
AciI CCGC 2 cut(s) 371, 767
AclI AACGTT 1 cut(s) 660
AclWI GGATC 6 cut(s) 81, 126, 211, 452, 768, 1029
AcsI RAATTY 2 cut(s) 5, 1172
AcuI CTGAAG 3 cut(s) 311, 729, 1158
AfeI AGCGCT 1 cut(s) 402
AfiI CCNNNNNNNGG 1 cut(s) 232
AgsI TTSAA 8 cut(s) 398, 451, 481, 494, 523, 530, 801, 830
AloI GAACNNNNNNTCC 2 cut(s) 923, 955
Alw21I GWGCWC 1 cut(s) 576
Alw26I GTCTC 4 cut(s) 61, 188, 1274, 1299
AlwI GGATC 6 cut(s) 81, 126, 211, 452, 768, 1029
Ama87I CYCGRG 1 cut(s) 1296
Aor51HI AGCGCT 1 cut(s) 402
AoxI GGCC 1 cut(s) 807
ApaI GGGCCC 1 cut(s) 811
ApeKI GCWGC 4 cut(s) 177, 368, 689, 1220
ApoI RAATTY 2 cut(s) 5, 1172
AspLEI GCGC 4 cut(s) 403, 420, 422, 1220
AspS9I GGNCC 2 cut(s) 807, 808
AsuC2I CCSGG 2 cut(s) 116, 1263
AsuHPI GGTGA 4 cut(s) 179, 223, 604, 1244
AsuII TTCGAA 1 cut(s) 1310
AvaI CYCGRG 1 cut(s) 1296
BaeGI GKGCMC 2 cut(s) 811, 1263
BanII GRGCYC 2 cut(s) 576, 811
BbsI GAAGAC 1 cut(s) 1212
Bbv12I GWGCWC 1 cut(s) 576
BbvI GCAGC 4 cut(s) 189, 355, 676, 1232
BccI CCATC 5 cut(s) 135, 200, 881, 919, 988
BceAI ACGGC 1 cut(s) 706
BcnI CCSGG 2 cut(s) 116, 1263
BcoDI GTCTC 4 cut(s) 61, 188, 1274, 1299
BfaI CTAG 1 cut(s) 843
BfoI RGCGCY 1 cut(s) 404
BfuAI ACCTGC 1 cut(s) 613
BisI GCNGC 5 cut(s) 178, 369, 690, 768, 1221
BlsI GCNGC 5 cut(s) 179, 370, 691, 769, 1222
Bme1390I CCNGG 2 cut(s) 116, 1263
BmeT110I CYCGRG 1 cut(s) 1296
BmgT120I GGNCC 2 cut(s) 807, 808
BmiI GGNNCC 1 cut(s) 809
BmrFI CCNGG 2 cut(s) 116, 1263
BmsI GCATC 5 cut(s) 256, 667, 907, 1024, 1060
BpiI GAAGAC 1 cut(s) 1212
Bpu10I CCTNAGC 1 cut(s) 575
Bpu14I TTCGAA 1 cut(s) 1310
BpuMI CCSGG 2 cut(s) 116, 1263
Bsa29I ATCGAT 1 cut(s) 915
BsaAI YACGTR 1 cut(s) 1112
BsaBI GATNNNNATC 1 cut(s) 759
BsaI GGTCTC 1 cut(s) 1299
BsaJI CCNNGG 3 cut(s) 231, 1055, 1197
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 1 cut(s) 232
Bse1I ACTGG 3 cut(s) 516, 609, 1245
Bse3DI GCAATG 3 cut(s) 160, 443, 837
Bse8I GATNNNNATC 1 cut(s) 759
BseCI ATCGAT 1 cut(s) 915
BseDI CCNNGG 3 cut(s) 231, 1055, 1197
BseGI GGATG 6 cut(s) 271, 682, 892, 985, 1051, 1186
BseJI GATNNNNATC 1 cut(s) 759
BseLI CCNNNNNNNGG 1 cut(s) 232
BseMI GCAATG 3 cut(s) 160, 443, 837
BseMII CTCAG 2 cut(s) 381, 589
BseNI ACTGG 3 cut(s) 516, 609, 1245
BsePI GCGCGC 1 cut(s) 418
BseRI GAGGAG 2 cut(s) 25, 206
BseSI GKGCMC 2 cut(s) 811, 1263
BseXI GCAGC 4 cut(s) 189, 355, 676, 1232
BseYI CCCAGC 1 cut(s) 750
BsgI GTGCAG 1 cut(s) 1104
Bsh1236I CGCG 2 cut(s) 420, 1082
Bsh1285I CGRYCG 1 cut(s) 915
BshFI GGCC 1 cut(s) 809
BshVI ATCGAT 1 cut(s) 915
BsiEI CGRYCG 1 cut(s) 915
BsiHKAI GWGCWC 1 cut(s) 576
BsiHKCI CYCGRG 1 cut(s) 1296
BsiSI CCGG 2 cut(s) 116, 1263
BslI CCNNNNNNNGG 1 cut(s) 232
BsmAI GTCTC 4 cut(s) 61, 188, 1274, 1299
BsmBI CGTCTC 1 cut(s) 188
BsnI GGCC 1 cut(s) 809
Bso31I GGTCTC 1 cut(s) 1299
BsoBI CYCGRG 1 cut(s) 1296
Bsp119I TTCGAA 1 cut(s) 1310
Bsp120I GGGCCC 1 cut(s) 807
Bsp1286I GDGCHC 3 cut(s) 576, 811, 1263
Bsp143I GATC 7 cut(s) 86, 118, 216, 457, 760, 912, 1021
Bsp68I TCGCGA 1 cut(s) 1082
BspACI CCGC 2 cut(s) 371, 767
BspANI GGCC 1 cut(s) 809
BspCNI CTCAG 2 cut(s) 382, 588
BspDI ATCGAT 1 cut(s) 915
BspFNI CGCG 2 cut(s) 420, 1082
BspHI TCATGA 1 cut(s) 706
BspLI GGNNCC 1 cut(s) 809
BspMI ACCTGC 1 cut(s) 613
BspPI GGATC 6 cut(s) 81, 126, 211, 452, 768, 1029
BspT104I TTCGAA 1 cut(s) 1310
BspTNI GGTCTC 1 cut(s) 1299
BsrDI GCAATG 3 cut(s) 160, 443, 837
BsrI ACTGG 3 cut(s) 516, 609, 1245
BssECI CCNNGG 3 cut(s) 231, 1055, 1197
BssHII GCGCGC 1 cut(s) 418
BssMI GATC 7 cut(s) 86, 118, 216, 457, 760, 912, 1021
Bst4CI ACNGT 1 cut(s) 778
BstBAI YACGTR 1 cut(s) 1112
BstBI TTCGAA 1 cut(s) 1310
BstC8I GCNNGC 4 cut(s) 333, 420, 563, 1049
BstDEI CTNAG 2 cut(s) 390, 575
BstDSI CCRYGG 2 cut(s) 231, 1197
BstF5I GGATG 6 cut(s) 271, 682, 892, 985, 1051, 1186
BstFNI CGCG 2 cut(s) 420, 1082
BstH2I RGCGCY 1 cut(s) 404
BstHHI GCGC 4 cut(s) 403, 420, 422, 1220
BstKTI GATC 7 cut(s) 89, 121, 219, 460, 763, 915, 1024
BstMAI GTCTC 4 cut(s) 61, 188, 1274, 1299
BstMBI GATC 7 cut(s) 86, 118, 216, 457, 760, 912, 1021
BstMCI CGRYCG 1 cut(s) 915
BstMWI GCNNNNNNNGC 6 cut(s) 400, 571, 686, 830, 839, 1088
BstSCI CCNGG 2 cut(s) 114, 1261
BstSLI GKGCMC 2 cut(s) 811, 1263
BstUI CGCG 2 cut(s) 420, 1082
BstV1I GCAGC 4 cut(s) 189, 355, 676, 1232
BstV2I GAAGAC 1 cut(s) 1212
BstX2I RGATCY 3 cut(s) 86, 216, 760
BstYI RGATCY 3 cut(s) 86, 216, 760
Bsu15I ATCGAT 1 cut(s) 915
BsuRI GGCC 1 cut(s) 809
BsuTUI ATCGAT 1 cut(s) 915
BtgI CCRYGG 2 cut(s) 231, 1197
BtgZI GCGATG 1 cut(s) 195
BtsCI GGATG 6 cut(s) 271, 682, 892, 985, 1051, 1186
BtsI GCAGTG 1 cut(s) 1116
BtsIMutI CAGTG 3 cut(s) 602, 783, 1116
BtuMI TCGCGA 1 cut(s) 1082
BveI ACCTGC 1 cut(s) 613
Cac8I GCNNGC 4 cut(s) 333, 420, 563, 1049
CciI TCATGA 1 cut(s) 706
CfoI GCGC 4 cut(s) 403, 420, 422, 1220
Cfr13I GGNCC 2 cut(s) 807, 808
ClaI ATCGAT 1 cut(s) 915
CseI GACGC 1 cut(s) 681
CspCI CAANNNNNGTGG 2 cut(s) 759, 794
CviAII CATG 1 cut(s) 707
DdeI CTNAG 2 cut(s) 390, 575
DpnI GATC 7 cut(s) 88, 120, 218, 459, 762, 914, 1023
DpnII GATC 7 cut(s) 86, 118, 216, 457, 760, 912, 1021
Ecl136II GAGCTC 1 cut(s) 574
Eco24I GRGCYC 2 cut(s) 576, 811
Eco31I GGTCTC 1 cut(s) 1299
Eco32I GATATC 2 cut(s) 475, 873
Eco47III AGCGCT 1 cut(s) 402
Eco53kI GAGCTC 1 cut(s) 574
Eco57I CTGAAG 3 cut(s) 311, 729, 1158
Eco88I CYCGRG 1 cut(s) 1296
EcoICRI GAGCTC 1 cut(s) 574
EcoO109I RGGNCCY 1 cut(s) 807
EcoRV GATATC 2 cut(s) 475, 873
EcoT22I ATGCAT 1 cut(s) 826
EcoT38I GRGCYC 2 cut(s) 576, 811
Esp3I CGTCTC 1 cut(s) 188
FaeI CATG 1 cut(s) 710
FalI AAGNNNNNCTT 2 cut(s) 959, 991
FatI CATG 1 cut(s) 706
FauNDI CATATG 1 cut(s) 1188
Fnu4HI GCNGC 5 cut(s) 178, 369, 690, 768, 1221
FokI GGATG 6 cut(s) 278, 689, 899, 972, 1038, 1193
FriOI GRGCYC 2 cut(s) 576, 811
Fsp4HI GCNGC 5 cut(s) 178, 369, 690, 768, 1221
FspBI CTAG 1 cut(s) 843
GlaI GCGC 4 cut(s) 402, 419, 421, 1219
GluI GCNGC 5 cut(s) 178, 369, 690, 768, 1221
GsaI CCCAGC 1 cut(s) 754
HaeII RGCGCY 1 cut(s) 404
HaeIII GGCC 1 cut(s) 809
HapII CCGG 2 cut(s) 116, 1263
HgaI GACGC 1 cut(s) 681
HhaI GCGC 4 cut(s) 403, 420, 422, 1220
Hin1II CATG 1 cut(s) 710
Hin6I GCGC 4 cut(s) 401, 418, 420, 1218
HinP1I GCGC 4 cut(s) 401, 418, 420, 1218
HindIII AAGCTT 1 cut(s) 741
HinfI GANTC 5 cut(s) 938, 954, 1030, 1133, 1193
HpaII CCGG 2 cut(s) 116, 1263
HphI GGTGA 4 cut(s) 179, 223, 604, 1244
Hpy166II GTNNAC 1 cut(s) 1235
Hpy188III TCNNGA 5 cut(s) 478, 674, 707, 1019, 1081
Hpy8I GTNNAC 1 cut(s) 1235
Hpy99I CGWCG 2 cut(s) 697, 994
HpyAV CCTTC 1 cut(s) 1208
HpyCH4III ACNGT 1 cut(s) 778
HpyCH4IV ACGT 3 cut(s) 318, 660, 1111
HpyF10VI GCNNNNNNNGC 6 cut(s) 400, 571, 686, 830, 839, 1088
HpyF3I CTNAG 2 cut(s) 390, 575
HpySE526I ACGT 3 cut(s) 318, 660, 1111
Hsp92II CATG 1 cut(s) 710
HspAI GCGC 4 cut(s) 401, 418, 420, 1218
Kzo9I GATC 7 cut(s) 86, 118, 216, 457, 760, 912, 1021
Lsp1109I GCAGC 4 cut(s) 189, 355, 676, 1232
LweI GCATC 5 cut(s) 256, 667, 907, 1024, 1060
MaeI CTAG 1 cut(s) 843
MaeII ACGT 3 cut(s) 318, 660, 1111
MaeIII GTNAC 2 cut(s) 184, 1107
MalI GATC 7 cut(s) 88, 120, 218, 459, 762, 914, 1023
MboI GATC 7 cut(s) 86, 118, 216, 457, 760, 912, 1021
MboII GAAGA 7 cut(s) 226, 367, 370, 493, 496, 652, 1217
MfeI CAATTG 1 cut(s) 1284
MflI RGATCY 3 cut(s) 86, 216, 760
MhlI GDGCHC 3 cut(s) 576, 811, 1263
MluCI AATT 7 cut(s) 5, 308, 380, 726, 1172, 1272, 1284
MlyI GAGTC 2 cut(s) 1024, 1202
MmeI TCCRAC 1 cut(s) 1013
Mph1103I ATGCAT 1 cut(s) 826
MseI TTAA 3 cut(s) 27, 540, 725
MslI CAYNNNNRTG 2 cut(s) 95, 165
MspA1I CMGCKG 1 cut(s) 1223
MspI CCGG 2 cut(s) 116, 1263
MspR9I CCNGG 2 cut(s) 116, 1263
MunI CAATTG 1 cut(s) 1284
MvnI CGCG 2 cut(s) 420, 1082
MwoI GCNNNNNNNGC 6 cut(s) 400, 571, 686, 830, 839, 1088
NciI CCSGG 2 cut(s) 116, 1263
NdeI CATATG 1 cut(s) 1188
NdeII GATC 7 cut(s) 86, 118, 216, 457, 760, 912, 1021
NlaIII CATG 1 cut(s) 710
NlaIV GGNNCC 1 cut(s) 809
NmuCI GTSAC 1 cut(s) 184
NruI TCGCGA 1 cut(s) 1082
NsiI ATGCAT 1 cut(s) 826
NspV TTCGAA 1 cut(s) 1310
PagI TCATGA 1 cut(s) 706
PauI GCGCGC 1 cut(s) 418
PfeI GAWTC 3 cut(s) 938, 954, 1133
PflMI CCANNNNNTGG 1 cut(s) 232
PkrI GCNGC 5 cut(s) 179, 370, 691, 769, 1222
Ple19I CGATCG 1 cut(s) 915
PleI GAGTC 2 cut(s) 1024, 1201
PpsI GAGTC 2 cut(s) 1024, 1201
Ppu21I YACGTR 1 cut(s) 1112
Psp124BI GAGCTC 1 cut(s) 576
Psp1406I AACGTT 1 cut(s) 660
PspFI CCCAGC 1 cut(s) 750
PspN4I GGNNCC 1 cut(s) 809
PspOMI GGGCCC 1 cut(s) 807
PspPI GGNCC 2 cut(s) 807, 808
PsuI RGATCY 3 cut(s) 86, 216, 760
PteI GCGCGC 1 cut(s) 418
PvuI CGATCG 1 cut(s) 915
PvuII CAGCTG 1 cut(s) 1223
RruI TCGCGA 1 cut(s) 1082
RseI CAYNNNNRTG 2 cut(s) 95, 165
SacI GAGCTC 1 cut(s) 576
SaqAI TTAA 3 cut(s) 27, 540, 725
SatI GCNGC 5 cut(s) 178, 369, 690, 768, 1221
Sau3AI GATC 7 cut(s) 86, 118, 216, 457, 760, 912, 1021
Sau96I GGNCC 2 cut(s) 807, 808
SchI GAGTC 2 cut(s) 1024, 1202
ScrFI CCNGG 2 cut(s) 116, 1263
SduI GDGCHC 3 cut(s) 576, 811, 1263
SfaNI GCATC 5 cut(s) 256, 667, 907, 1024, 1060
SfuI TTCGAA 1 cut(s) 1310
SmiMI CAYNNNNRTG 2 cut(s) 95, 165
Sse9I AATT 7 cut(s) 5, 308, 380, 726, 1172, 1272, 1284
SsiI CCGC 2 cut(s) 371, 767
SspI AATATT 2 cut(s) 1077, 1149
SspMI CTAG 1 cut(s) 843
SstI GAGCTC 1 cut(s) 576
StyD4I CCNGG 2 cut(s) 114, 1261
TaaI ACNGT 1 cut(s) 778
TaiI ACGT 3 cut(s) 321, 663, 1114
TaqI TCGA 4 cut(s) 695, 915, 1158, 1310
TasI AATT 7 cut(s) 5, 308, 380, 726, 1172, 1272, 1284
TauI GCSGC 1 cut(s) 770
TfiI GAWTC 3 cut(s) 938, 954, 1133
Tru1I TTAA 3 cut(s) 27, 540, 725
Tru9I TTAA 3 cut(s) 27, 540, 725
TscAI CASTG 3 cut(s) 609, 783, 1123
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 4 cut(s) 177, 368, 689, 1220
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 5 cut(s) 428, 695, 752, 974, 1053
TspGWI ACGGA 2 cut(s) 212, 1186
TspRI CASTG 3 cut(s) 609, 783, 1123
Van91I CCANNNNNTGG 1 cut(s) 232
XapI RAATTY 2 cut(s) 5, 1172
XspI CTAG 1 cut(s) 843
Zsp2I ATGCAT 1 cut(s) 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.