Rw5G044180

Plant protein of unknown function (DUF641)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011946
Physical Location & Seq
Forward (+)
77776684 .. 77778302
1619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G044180.1

Sequence Viewer

Length: 1284 bp
ATGGAATCTGCTGGGGCTAAACCCCTTAAACCCTCCTCAAACATATCTGATATTGTCTCCAAGTTTGCCAAAGTTTGCAAATTGAGATCCATTGGAGTGCACCAACCTCACAACAGCAATAATGCTCCTTCGTGTGAAGATGGCAGCGATGTGACTGAGGACACCGAGTGTGGTGGGGTGAAGGTCCATCCCCAACCTGTTGAAGTTACAAGAGAAAGTAAAATATGTGGAGATGAGGAGTTATCCAAGTTATTCGACATTGTTTCGACGCTGAAGTTGGCTTATGTTCAGCTCCAGGAAGCTCATTTACCCTACAACCCGAAAAAGATTATAGCTGCAGATGAGCGCTTTATGGCTGAGCTTGAAGCAATATGCAAGATGAAGCGCGCATATAAGGAGAAGCAGGTTGTGAAGCTGAAGTCAGAGTCCTCTCGTTCTGATATTCTCAGGAAGAAAATTGAATTGAATGAGAAGCTGCTAGAAGAGTTTAAGTTGCAAATGGAAGTAAACAATTCTGAGATAATCTGCCTGCAAAAAGAGCGCAGGGATTTGGATTTGGTGAATGCAACACTGGCTGAGAAGGTAAGGCAGATAAGTTTGCAAAGGAAGACTGCAAGGGTTTGGACCATTACAAGATTTCAGGATACTTTCAGAGCTGCATCGAAATCAATTCATGACTTCGCAAAGCCTTTAATCAGCTTGATGAAAGCCACAGGATGGGACTTGGATCTGGCGGCAAGGGCAGTAGAAGCTGAAGCTGCTTACTTGAAAAGGTCCCACAAGAAGTATGCATTTGAAGCTTACATTGCAAGGAGAATGTTTTATGGGTTATCATTAAAATCTTGTAATGTGGATGGTATTATGAGATATGATGATCCCCTCGATGCCTTGATTGAGAACCCAGACTCAGATTTTGCCCAATATTGTGGAGAAAAGTATCTACAAGTTGTTCATCCAATGATGGAAACGCGGTTCTTTGGGAATCTGGATCACAGGACTCTTTTATTGAGTGGCAAGCATCCAAGAACGTCATTCTACCAAATATTTGCAAGAATGGCAAGGTGGGTTTGGGTTTTGCACAGCATTGCACCTATGATAGATTCTGAAGCAAAAATGTTTGCTGTCAAAAGAGGAAGCAAATTCTCACATGTTTATATGGAGTTGGTGGTGGAGGATGGGGAAGATGCGGCTGTCTCAGATGGAGGACAGGTCGAGTTTATGGTGATGCCAGGATTTAGAATGTTAGAGACATTAGTGAAGTCTCGTGTGTATCTTTCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF641

Protein Analysis

427

Amino Acids

48.66

Weight (kDa)

8.58

Isoelectric Point (pI)

44.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF641 PF04859 78 - 192 1.1e-23 Plant protein of unknown function (DUF641)
GIL1_IRKI_C PF24994 372 - 424 6e-18 GIL1/IRKI C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011946)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30380
fragaria_vesca FvH4_3g40290
malus_domestica MD03G1053800.v1.1 MD11G1055400.v1.1
prunus_persica Prupe.6G042900_v2.0.a1 Prupe.6G042900_v2.0.a1 Prupe.6G042900_v2.0.a1 Prupe.6G042900_v2.0.a1
pyrus_communis pycom03g04270
rosa_chinensis RchiOBHm_Chr5g0072631
rosa_laevigata RLG00000036318
rosa_multiflora Rmu_sc0002088.1_g000004
rosa_roxburghii Rroxscaffold_1G00008520
rosa_rugosa Rorug05G0418900
rosa_samantha Rh5AG475800 Rh5BG495900 Rh5CG519700 Rh5DG508400
rosa_wichuraiana Rw5G044180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 394
AccB7I CCANNNNNTGG 1 cut(s) 717
AccII CGCG 2 cut(s) 387, 970
AciI CCGC 3 cut(s) 734, 970, 1187
AclWI GGATC 4 cut(s) 81, 735, 869, 996
AcsI RAATTY 1 cut(s) 1139
AcuI CTGAAG 4 cut(s) 293, 437, 774, 1125
AdeI CACNNNGTG 1 cut(s) 168
AfeI AGCGCT 1 cut(s) 347
AfiI CCNNNNNNNGG 1 cut(s) 717
AflIII ACRYGT 1 cut(s) 1147
AgsI TTSAA 7 cut(s) 203, 365, 461, 466, 769, 797, 1278
AjnI CCWGG 2 cut(s) 294, 1228
AloI GAACNNNNNNTCC 2 cut(s) 956, 988
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 4 cut(s) 61, 1198, 1241, 1266
Alw44I GTGCAC 1 cut(s) 98
AlwI GGATC 4 cut(s) 81, 735, 869, 996
Aor51HI AGCGCT 1 cut(s) 347
ApaLI GTGCAC 1 cut(s) 98
ApeKI GCWGC 5 cut(s) 144, 335, 475, 656, 758
ApoI RAATTY 1 cut(s) 1139
ArsI GACNNNNNNTTYG 2 cut(s) 896, 928
AspLEI GCGC 4 cut(s) 348, 387, 389, 543
AspS9I GGNCC 3 cut(s) 184, 624, 774
AsuHPI GGTGA 3 cut(s) 190, 571, 1234
AvaII GGWCC 3 cut(s) 184, 624, 774
BaeGI GKGCMC 1 cut(s) 102
BarI GAAGNNNNNNTAC 2 cut(s) 291, 323
BauI CACGAG 1 cut(s) 1263
BbsI GAAGAC 1 cut(s) 614
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 5 cut(s) 156, 322, 462, 643, 745
BccI CCATC 7 cut(s) 134, 195, 711, 848, 955, 1169, 1193
BciT130I CCWGG 2 cut(s) 296, 1230
BciVI GTATCC 1 cut(s) 637
BcoDI GTCTC 4 cut(s) 61, 1198, 1241, 1266
BfaI CTAG 1 cut(s) 479
BfmI CTRYAG 1 cut(s) 336
BfoI RGCGCY 1 cut(s) 349
BfuAI ACCTGC 1 cut(s) 394
BfuI GTATCC 1 cut(s) 637
BisI GCNGC 7 cut(s) 145, 336, 476, 657, 735, 759, 1188
BlpI GCTNAGC 1 cut(s) 357
BlsI GCNGC 7 cut(s) 146, 337, 477, 658, 736, 760, 1189
Bme1390I CCNGG 2 cut(s) 296, 1230
Bme18I GGWCC 3 cut(s) 184, 624, 774
BmgT120I GGNCC 3 cut(s) 184, 624, 774
BmiI GGNNCC 1 cut(s) 776
BmrFI CCNGG 2 cut(s) 296, 1230
BmsI GCATC 5 cut(s) 668, 874, 1027, 1174, 1215
BpiI GAAGAC 1 cut(s) 614
BpmI CTGGAG 1 cut(s) 278
Bpu1102I GCTNAGC 1 cut(s) 357
BsaXI ACNNNNNCTCC 2 cut(s) 1194, 1224
Bsc4I CCNNNNNNNGG 1 cut(s) 717
Bse1I ACTGG 1 cut(s) 576
Bse3DI GCAATG 2 cut(s) 804, 1083
BseBI CCWGG 2 cut(s) 296, 1230
BseGI GGATG 6 cut(s) 187, 722, 859, 952, 1018, 1180
BseLI CCNNNNNNNGG 1 cut(s) 717
BseMI GCAATG 2 cut(s) 804, 1083
BseMII CTCAG 7 cut(s) 147, 348, 460, 507, 567, 921, 1209
BseNI ACTGG 1 cut(s) 576
BsePI GCGCGC 1 cut(s) 385
BseRI GAGGAG 2 cut(s) 25, 251
BseSI GKGCMC 1 cut(s) 102
BseXI GCAGC 5 cut(s) 156, 322, 462, 643, 745
BseYI CCCAGC 1 cut(s) 11
Bsh1236I CGCG 2 cut(s) 387, 970
BsiHKAI GWGCWC 1 cut(s) 102
BslFI GGGAC 2 cut(s) 734, 760
BslI CCNNNNNNNGG 1 cut(s) 717
BsmAI GTCTC 4 cut(s) 61, 1198, 1241, 1266
BsmFI GGGAC 2 cut(s) 734, 760
BsmI GAATGC 1 cut(s) 568
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 4 cut(s) 86, 727, 874, 988
Bsp1720I GCTNAGC 1 cut(s) 357
BspACI CCGC 3 cut(s) 734, 970, 1187
BspCNI CTCAG 7 cut(s) 148, 349, 459, 508, 568, 920, 1208
BspFNI CGCG 2 cut(s) 387, 970
BspHI TCATGA 1 cut(s) 673
BspLI GGNNCC 1 cut(s) 776
BspMAI CTGCAG 1 cut(s) 340
BspMI ACCTGC 1 cut(s) 394
BspPI GGATC 4 cut(s) 81, 735, 869, 996
BsrDI GCAATG 2 cut(s) 804, 1083
BsrI ACTGG 1 cut(s) 576
BssHII GCGCGC 1 cut(s) 385
BssMI GATC 4 cut(s) 86, 727, 874, 988
BssSI CACGAG 1 cut(s) 1263
Bst2BI CACGAG 1 cut(s) 1263
Bst2UI CCWGG 2 cut(s) 296, 1230
Bst6I CTCTTC 1 cut(s) 477
BstC8I GCNNGC 3 cut(s) 387, 530, 1016
BstDEI CTNAG 7 cut(s) 156, 357, 446, 516, 576, 907, 1195
BstF5I GGATG 6 cut(s) 187, 722, 859, 952, 1018, 1180
BstFNI CGCG 2 cut(s) 387, 970
BstH2I RGCGCY 1 cut(s) 349
BstHHI GCGC 4 cut(s) 348, 387, 389, 543
BstKTI GATC 4 cut(s) 89, 730, 877, 991
BstMAI GTCTC 4 cut(s) 61, 1198, 1241, 1266
BstMBI GATC 4 cut(s) 86, 727, 874, 988
BstMWI GCNNNNNNNGC 8 cut(s) 538, 572, 740, 749, 758, 797, 806, 1055
BstNI CCWGG 2 cut(s) 296, 1230
BstNSI RCATGY 1 cut(s) 1151
BstSCI CCNGG 2 cut(s) 294, 1228
BstSFI CTRYAG 1 cut(s) 336
BstSLI GKGCMC 1 cut(s) 102
BstUI CGCG 2 cut(s) 387, 970
BstV1I GCAGC 5 cut(s) 156, 322, 462, 643, 745
BstV2I GAAGAC 1 cut(s) 614
BstX2I RGATCY 2 cut(s) 86, 727
BstXI CCANNNNNNTGG 1 cut(s) 926
BstYI RGATCY 2 cut(s) 86, 727
BsuI GTATCC 1 cut(s) 637
BtgZI GCGATG 1 cut(s) 162
BtsCI GGATG 6 cut(s) 187, 722, 859, 952, 1018, 1180
BtsIMutI CAGTG 1 cut(s) 569
BveI ACCTGC 1 cut(s) 394
Cac8I GCNNGC 3 cut(s) 387, 530, 1016
CciI TCATGA 1 cut(s) 673
CfoI GCGC 4 cut(s) 348, 387, 389, 543
Cfr13I GGNCC 3 cut(s) 184, 624, 774
CseI GACGC 1 cut(s) 277
CviAII CATG 2 cut(s) 674, 1148
DdeI CTNAG 7 cut(s) 156, 357, 446, 516, 576, 907, 1195
DpnI GATC 4 cut(s) 88, 729, 876, 990
DpnII GATC 4 cut(s) 86, 727, 874, 988
DraIII CACNNNGTG 1 cut(s) 168
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
Eco47I GGWCC 3 cut(s) 184, 624, 774
Eco47III AGCGCT 1 cut(s) 347
Eco57I CTGAAG 4 cut(s) 293, 437, 774, 1125
EcoO109I RGGNCCY 1 cut(s) 774
EcoRII CCWGG 2 cut(s) 294, 1228
EcoT22I ATGCAT 1 cut(s) 793
FaeI CATG 2 cut(s) 677, 1151
FaqI GGGAC 2 cut(s) 734, 760
FatI CATG 2 cut(s) 673, 1147
Fnu4HI GCNGC 7 cut(s) 145, 336, 476, 657, 735, 759, 1188
FokI GGATG 6 cut(s) 174, 729, 866, 939, 1005, 1187
Fsp4HI GCNGC 7 cut(s) 145, 336, 476, 657, 735, 759, 1188
FspBI CTAG 1 cut(s) 479
GlaI GCGC 4 cut(s) 347, 386, 388, 542
GluI GCNGC 7 cut(s) 145, 336, 476, 657, 735, 759, 1188
GsaI CCCAGC 1 cut(s) 15
GsuI CTGGAG 1 cut(s) 278
HaeII RGCGCY 1 cut(s) 349
HgaI GACGC 1 cut(s) 277
HhaI GCGC 4 cut(s) 348, 387, 389, 543
Hin1II CATG 2 cut(s) 677, 1151
Hin6I GCGC 4 cut(s) 346, 385, 387, 541
HinP1I GCGC 4 cut(s) 346, 385, 387, 541
HindIII AAGCTT 1 cut(s) 798
HinfI GANTC 6 cut(s) 5, 425, 905, 982, 997, 1100
HphI GGTGA 3 cut(s) 190, 571, 1234
Hpy166II GTNNAC 2 cut(s) 100, 508
Hpy188I TCNGA 8 cut(s) 49, 424, 439, 517, 653, 910, 1105, 1198
Hpy188III TCNNGA 4 cut(s) 448, 641, 674, 986
Hpy8I GTNNAC 2 cut(s) 100, 508
Hpy99I CGWCG 1 cut(s) 271
HpyAV CCTTC 3 cut(s) 138, 175, 574
HpyCH4IV ACGT 1 cut(s) 1028
HpyF10VI GCNNNNNNNGC 8 cut(s) 538, 572, 740, 749, 758, 797, 806, 1055
HpyF3I CTNAG 7 cut(s) 156, 357, 446, 516, 576, 907, 1195
HpySE526I ACGT 1 cut(s) 1028
Hsp92II CATG 2 cut(s) 677, 1151
HspAI GCGC 4 cut(s) 346, 385, 387, 541
Kzo9I GATC 4 cut(s) 86, 727, 874, 988
LmnI GCTCC 2 cut(s) 130, 297
Lsp1109I GCAGC 5 cut(s) 156, 322, 462, 643, 745
LweI GCATC 5 cut(s) 668, 874, 1027, 1174, 1215
MaeI CTAG 1 cut(s) 479
MaeII ACGT 1 cut(s) 1028
MaeIII GTNAC 2 cut(s) 151, 205
MalI GATC 4 cut(s) 88, 729, 876, 990
MboI GATC 4 cut(s) 86, 727, 874, 988
MboII GAAGA 5 cut(s) 149, 463, 494, 619, 1193
MflI RGATCY 2 cut(s) 86, 727
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 6 cut(s) 80, 456, 461, 511, 669, 1139
MlyI GAGTC 3 cut(s) 434, 899, 991
Mph1103I ATGCAT 1 cut(s) 793
MseI TTAA 4 cut(s) 27, 489, 692, 836
MslI CAYNNNNRTG 1 cut(s) 95
MspR9I CCNGG 2 cut(s) 296, 1230
Mva1269I GAATGC 1 cut(s) 568
MvaI CCWGG 2 cut(s) 296, 1230
MvnI CGCG 2 cut(s) 387, 970
MwoI GCNNNNNNNGC 8 cut(s) 538, 572, 740, 749, 758, 797, 806, 1055
NdeII GATC 4 cut(s) 86, 727, 874, 988
NlaIII CATG 2 cut(s) 677, 1151
NlaIV GGNNCC 1 cut(s) 776
NmuCI GTSAC 1 cut(s) 151
NsiI ATGCAT 1 cut(s) 793
NspI RCATGY 1 cut(s) 1151
PagI TCATGA 1 cut(s) 673
PauI GCGCGC 1 cut(s) 385
PciI ACATGT 1 cut(s) 1147
PctI GAATGC 1 cut(s) 568
PfeI GAWTC 3 cut(s) 5, 982, 1100
PflMI CCANNNNNTGG 1 cut(s) 717
PfoI TCCNGGA 1 cut(s) 294
PkrI GCNGC 7 cut(s) 146, 337, 477, 658, 736, 760, 1189
PleI GAGTC 3 cut(s) 433, 899, 991
PpsI GAGTC 3 cut(s) 433, 899, 991
PpuMI RGGWCCY 1 cut(s) 774
PscI ACATGT 1 cut(s) 1147
Psp5II RGGWCCY 1 cut(s) 774
Psp6I CCWGG 2 cut(s) 294, 1228
PspFI CCCAGC 1 cut(s) 11
PspGI CCWGG 2 cut(s) 294, 1228
PspN4I GGNNCC 1 cut(s) 776
PspPI GGNCC 3 cut(s) 184, 624, 774
PspPPI RGGWCCY 1 cut(s) 774
PstI CTGCAG 1 cut(s) 340
PsuI RGATCY 2 cut(s) 86, 727
PteI GCGCGC 1 cut(s) 385
RseI CAYNNNNRTG 1 cut(s) 95
SaqAI TTAA 4 cut(s) 27, 489, 692, 836
SatI GCNGC 7 cut(s) 145, 336, 476, 657, 735, 759, 1188
Sau3AI GATC 4 cut(s) 86, 727, 874, 988
Sau96I GGNCC 3 cut(s) 184, 624, 774
SchI GAGTC 3 cut(s) 434, 899, 991
ScrFI CCNGG 2 cut(s) 296, 1230
SduI GDGCHC 1 cut(s) 102
SfaNI GCATC 5 cut(s) 668, 874, 1027, 1174, 1215
SfcI CTRYAG 1 cut(s) 336
SinI GGWCC 3 cut(s) 184, 624, 774
SmiMI CAYNNNNRTG 1 cut(s) 95
Sse9I AATT 6 cut(s) 80, 456, 461, 511, 669, 1139
SsiI CCGC 3 cut(s) 734, 970, 1187
SspI AATATT 2 cut(s) 923, 1044
SspMI CTAG 1 cut(s) 479
StyD4I CCNGG 2 cut(s) 294, 1228
TaiI ACGT 1 cut(s) 1031
TaqI TCGA 5 cut(s) 255, 266, 662, 882, 1212
TasI AATT 6 cut(s) 80, 456, 461, 511, 669, 1139
TauI GCSGC 2 cut(s) 737, 1190
TfiI GAWTC 3 cut(s) 5, 982, 1100
Tru1I TTAA 4 cut(s) 27, 489, 692, 836
Tru9I TTAA 4 cut(s) 27, 489, 692, 836
TscAI CASTG 1 cut(s) 576
TseFI GTSAC 1 cut(s) 151
TseI GCWGC 5 cut(s) 144, 335, 475, 656, 758
Tsp45I GTSAC 1 cut(s) 151
TspDTI ATGAA 4 cut(s) 395, 662, 719, 941
TspRI CASTG 1 cut(s) 576
Van91I CCANNNNNTGG 1 cut(s) 717
VneI GTGCAC 1 cut(s) 98
VpaK11BI GGWCC 3 cut(s) 184, 624, 774
XapI RAATTY 1 cut(s) 1139
XceI RCATGY 1 cut(s) 1151
XspI CTAG 1 cut(s) 479
Zsp2I ATGCAT 1 cut(s) 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.