Rh5AG475800

Plant protein of unknown function (DUF641)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0011946
Physical Location & Seq
Forward (+)
81761947 .. 81764950
3004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG475800.1

Sequence Viewer

Length: 1287 bp
ATGGAATCTGCTGGGGCTAATAAACCCCTTAAACCCTCCTCAAACATATCTGATATTGTCTCCAAGTTTGCCAAAGTTTGCAAATTGAGATCCATTGGAGTGCACCAACCTCACAACAGCAATAATGCTCCTTCGTGTGAAGATGGCAGCGATGTGACTGAGGACACCGAGTGTGGTGGGGTGAAGGTCCATCCCCAACCTGTTGAAGTTACAAGAGAAAGCAAAATATGTGGAGATGAGGAGTTATCCAAGTTATTCGACATTGTTTCGACGCTGAAGTTGGCTTATGTTCAGCTCCAGGAAGCTCATTTACCCTACAACCCGAAAAAGATTATAGCTGCAGATGAGCGTTTTATGGCTGAGCTTGAAGCAATATGCAAGATGAAGCGCGCATATAAGGAGAAGCAGTTTGTGAAGCTGAAGTCAGAGTCCTCTCGTTCTGATATTCTCAGGAAGAAAATTGAATTGAATGAGAAGCTGCTAGAAGAGTTTAAGTTGCAAATGGAAGTAAACAATTCTGAGATAATCTGCCTGCAAAAAGAGCTCAGGGATTTGGATTTGGTGAATGCGACACTGGCTGAGAAGGTAAGGCAGATAAGTTTGCAAAGGAAGACTGCAAGGGTTTGGACCATTACAAGATTTCAGGATACTTTCAGAGCTGCATCGAAATCAATTCATGACTTCGCAAAGCCTTTAATCAGCTTGATGAAAGCCACAGGATGGGACTTGGATCTGGCGGCAAGGGAAGTAGAAGCTGAAGCTGCTTACTTGAAAAGGTCCCACAAGAAGTATGCATTTGAAGCTTACATTGCAAGGAGAATGTTTTATGGGTTATCATTAAAATCTTGTAATGTGGATGGTATTATGAGATATGATGATCCCATCGATGCCTTGATTGAGAACCCAGACTCAGATTTTGCCCGATATTGTGGAGAAAAGTATCTACAAGTTGTTCATCCAATGATGGAAACGCGGTTCTTTGGGAATCTGGATCACAGGACGCTTGTATTGAGTGGCAAGCATCCAAGAACGTCATTCTACCAAATATTTGCAAGAATGGCAAGGTGGGTTTGGGTTTTGCACAGCATTGCACCTATGATAGATTCTGAAGCAAAAATGTTTGCTGTCAAAAGAGGAAGCAAATTCTCACATGTTTATATGGAGTTGGTGGTGGAGGATGGGGAAGATGCAGCTGTCTCAGATGGAGGAAAGGTCGAGTTTATGGTGATGCCAGGATTTCGAATGTTAGAGACATTAGTGAAGTCTCGTGTGTATCTTTCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF641

Protein Analysis

428

Amino Acids

48.86

Weight (kDa)

8.57

Isoelectric Point (pI)

43.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF641 PF04859 79 - 195 2e-25 Plant protein of unknown function (DUF641)
GIL1_IRKI_C PF24994 373 - 425 6.1e-18 GIL1/IRKI C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011946)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30380
fragaria_vesca FvH4_3g40290
malus_domestica MD03G1053800.v1.1 MD11G1055400.v1.1
prunus_persica Prupe.6G042900_v2.0.a1 Prupe.6G042900_v2.0.a1 Prupe.6G042900_v2.0.a1 Prupe.6G042900_v2.0.a1
pyrus_communis pycom03g04270
rosa_chinensis RchiOBHm_Chr5g0072631
rosa_laevigata RLG00000036318
rosa_multiflora Rmu_sc0002088.1_g000004
rosa_roxburghii Rroxscaffold_1G00008520
rosa_rugosa Rorug05G0418900
rosa_samantha Rh5AG475800 Rh5BG495900 Rh5CG519700 Rh5DG508400
rosa_wichuraiana Rw5G044180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 720
AccII CGCG 2 cut(s) 390, 973
AciI CCGC 2 cut(s) 737, 973
AclWI GGATC 4 cut(s) 84, 738, 872, 999
AcsI RAATTY 1 cut(s) 1142
AcuI CTGAAG 4 cut(s) 296, 440, 777, 1128
AdeI CACNNNGTG 1 cut(s) 171
AfiI CCNNNNNNNGG 1 cut(s) 720
AflIII ACRYGT 1 cut(s) 1150
AgsI TTSAA 7 cut(s) 206, 368, 464, 469, 772, 800, 1281
AjnI CCWGG 2 cut(s) 297, 1231
AloI GAACNNNNNNTCC 2 cut(s) 959, 991
Alw21I GWGCWC 2 cut(s) 105, 546
Alw26I GTCTC 4 cut(s) 64, 1201, 1244, 1269
Alw44I GTGCAC 1 cut(s) 101
AlwI GGATC 4 cut(s) 84, 738, 872, 999
ApaLI GTGCAC 1 cut(s) 101
ApeKI GCWGC 6 cut(s) 147, 338, 478, 659, 761, 1190
ApoI RAATTY 1 cut(s) 1142
ArsI GACNNNNNNTTYG 2 cut(s) 899, 931
AspLEI GCGC 2 cut(s) 390, 392
AspS9I GGNCC 3 cut(s) 187, 627, 777
AsuHPI GGTGA 3 cut(s) 193, 574, 1237
AsuII TTCGAA 1 cut(s) 1240
AvaII GGWCC 3 cut(s) 187, 627, 777
BaeGI GKGCMC 1 cut(s) 105
BanII GRGCYC 1 cut(s) 546
BarI GAAGNNNNNNTAC 2 cut(s) 294, 326
BauI CACGAG 1 cut(s) 1266
BbsI GAAGAC 1 cut(s) 617
Bbv12I GWGCWC 2 cut(s) 105, 546
BbvI GCAGC 6 cut(s) 159, 325, 465, 646, 748, 1202
BccI CCATC 8 cut(s) 137, 198, 714, 851, 890, 958, 1172, 1196
BciT130I CCWGG 2 cut(s) 299, 1233
BciVI GTATCC 1 cut(s) 640
BcoDI GTCTC 4 cut(s) 64, 1201, 1244, 1269
BfaI CTAG 1 cut(s) 482
BfmI CTRYAG 1 cut(s) 339
BfuI GTATCC 1 cut(s) 640
BisI GCNGC 7 cut(s) 148, 339, 479, 660, 738, 762, 1191
BlpI GCTNAGC 1 cut(s) 360
BlsI GCNGC 7 cut(s) 149, 340, 480, 661, 739, 763, 1192
Bme1390I CCNGG 2 cut(s) 299, 1233
Bme18I GGWCC 3 cut(s) 187, 627, 777
BmgT120I GGNCC 3 cut(s) 187, 627, 777
BmiI GGNNCC 1 cut(s) 779
BmrFI CCNGG 2 cut(s) 299, 1233
BmsI GCATC 5 cut(s) 671, 877, 1030, 1177, 1218
BpiI GAAGAC 1 cut(s) 617
BpmI CTGGAG 1 cut(s) 281
Bpu10I CCTNAGC 1 cut(s) 545
Bpu1102I GCTNAGC 1 cut(s) 360
Bpu14I TTCGAA 1 cut(s) 1240
Bsa29I ATCGAT 1 cut(s) 885
BsaXI ACNNNNNCTCC 4 cut(s) 392, 422, 1197, 1227
Bsc4I CCNNNNNNNGG 1 cut(s) 720
Bse1I ACTGG 1 cut(s) 579
Bse3DI GCAATG 2 cut(s) 807, 1086
BseBI CCWGG 2 cut(s) 299, 1233
BseCI ATCGAT 1 cut(s) 885
BseGI GGATG 6 cut(s) 190, 725, 862, 955, 1021, 1183
BseLI CCNNNNNNNGG 1 cut(s) 720
BseMI GCAATG 2 cut(s) 807, 1086
BseMII CTCAG 8 cut(s) 150, 351, 463, 510, 559, 570, 924, 1212
BseNI ACTGG 1 cut(s) 579
BsePI GCGCGC 1 cut(s) 388
BseRI GAGGAG 2 cut(s) 28, 254
BseSI GKGCMC 1 cut(s) 105
BseXI GCAGC 6 cut(s) 159, 325, 465, 646, 748, 1202
BseYI CCCAGC 1 cut(s) 11
Bsh1236I CGCG 2 cut(s) 390, 973
BshVI ATCGAT 1 cut(s) 885
BsiHKAI GWGCWC 2 cut(s) 105, 546
BslFI GGGAC 2 cut(s) 737, 763
BslI CCNNNNNNNGG 1 cut(s) 720
BsmAI GTCTC 4 cut(s) 64, 1201, 1244, 1269
BsmFI GGGAC 2 cut(s) 737, 763
BsmI GAATGC 1 cut(s) 571
Bsp119I TTCGAA 1 cut(s) 1240
Bsp1286I GDGCHC 2 cut(s) 105, 546
Bsp143I GATC 4 cut(s) 89, 730, 877, 991
Bsp1720I GCTNAGC 1 cut(s) 360
BspACI CCGC 2 cut(s) 737, 973
BspCNI CTCAG 8 cut(s) 151, 352, 462, 511, 558, 571, 923, 1211
BspDI ATCGAT 1 cut(s) 885
BspFNI CGCG 2 cut(s) 390, 973
BspHI TCATGA 1 cut(s) 676
BspLI GGNNCC 1 cut(s) 779
BspMAI CTGCAG 1 cut(s) 343
BspPI GGATC 4 cut(s) 84, 738, 872, 999
BspT104I TTCGAA 1 cut(s) 1240
BsrDI GCAATG 2 cut(s) 807, 1086
BsrI ACTGG 1 cut(s) 579
BssHII GCGCGC 1 cut(s) 388
BssMI GATC 4 cut(s) 89, 730, 877, 991
BssSI CACGAG 1 cut(s) 1266
Bst2BI CACGAG 1 cut(s) 1266
Bst2UI CCWGG 2 cut(s) 299, 1233
Bst6I CTCTTC 1 cut(s) 480
BstBI TTCGAA 1 cut(s) 1240
BstC8I GCNNGC 3 cut(s) 390, 533, 1019
BstDEI CTNAG 8 cut(s) 159, 360, 449, 519, 545, 579, 910, 1198
BstF5I GGATG 6 cut(s) 190, 725, 862, 955, 1021, 1183
BstFNI CGCG 2 cut(s) 390, 973
BstHHI GCGC 2 cut(s) 390, 392
BstKTI GATC 4 cut(s) 92, 733, 880, 994
BstMAI GTCTC 4 cut(s) 64, 1201, 1244, 1269
BstMBI GATC 4 cut(s) 89, 730, 877, 991
BstMWI GCNNNNNNNGC 6 cut(s) 541, 575, 761, 800, 809, 1058
BstNI CCWGG 2 cut(s) 299, 1233
BstNSI RCATGY 1 cut(s) 1154
BstSCI CCNGG 2 cut(s) 297, 1231
BstSFI CTRYAG 1 cut(s) 339
BstSLI GKGCMC 1 cut(s) 105
BstUI CGCG 2 cut(s) 390, 973
BstV1I GCAGC 6 cut(s) 159, 325, 465, 646, 748, 1202
BstV2I GAAGAC 1 cut(s) 617
BstX2I RGATCY 2 cut(s) 89, 730
BstYI RGATCY 2 cut(s) 89, 730
Bsu15I ATCGAT 1 cut(s) 885
BsuI GTATCC 1 cut(s) 640
BsuTUI ATCGAT 1 cut(s) 885
BtgZI GCGATG 1 cut(s) 165
BtsCI GGATG 6 cut(s) 190, 725, 862, 955, 1021, 1183
BtsIMutI CAGTG 1 cut(s) 572
Cac8I GCNNGC 3 cut(s) 390, 533, 1019
CciI TCATGA 1 cut(s) 676
CfoI GCGC 2 cut(s) 390, 392
Cfr13I GGNCC 3 cut(s) 187, 627, 777
ClaI ATCGAT 1 cut(s) 885
CseI GACGC 2 cut(s) 280, 1009
CspCI CAANNNNNGTGG 2 cut(s) 211, 246
CviAII CATG 2 cut(s) 677, 1151
DdeI CTNAG 8 cut(s) 159, 360, 449, 519, 545, 579, 910, 1198
DpnI GATC 4 cut(s) 91, 732, 879, 993
DpnII GATC 4 cut(s) 89, 730, 877, 991
DraIII CACNNNGTG 1 cut(s) 171
Eam1104I CTCTTC 1 cut(s) 480
EarI CTCTTC 1 cut(s) 480
Ecl136II GAGCTC 1 cut(s) 544
Eco24I GRGCYC 1 cut(s) 546
Eco47I GGWCC 3 cut(s) 187, 627, 777
Eco53kI GAGCTC 1 cut(s) 544
Eco57I CTGAAG 4 cut(s) 296, 440, 777, 1128
EcoICRI GAGCTC 1 cut(s) 544
EcoO109I RGGNCCY 1 cut(s) 777
EcoRII CCWGG 2 cut(s) 297, 1231
EcoT22I ATGCAT 1 cut(s) 796
EcoT38I GRGCYC 1 cut(s) 546
FaeI CATG 2 cut(s) 680, 1154
FaqI GGGAC 2 cut(s) 737, 763
FatI CATG 2 cut(s) 676, 1150
Fnu4HI GCNGC 7 cut(s) 148, 339, 479, 660, 738, 762, 1191
FokI GGATG 6 cut(s) 177, 732, 869, 942, 1008, 1190
FriOI GRGCYC 1 cut(s) 546
Fsp4HI GCNGC 7 cut(s) 148, 339, 479, 660, 738, 762, 1191
FspBI CTAG 1 cut(s) 482
GlaI GCGC 2 cut(s) 389, 391
GluI GCNGC 7 cut(s) 148, 339, 479, 660, 738, 762, 1191
GsaI CCCAGC 1 cut(s) 15
GsuI CTGGAG 1 cut(s) 281
HgaI GACGC 2 cut(s) 280, 1009
HhaI GCGC 2 cut(s) 390, 392
Hin1II CATG 2 cut(s) 680, 1154
Hin6I GCGC 2 cut(s) 388, 390
HinP1I GCGC 2 cut(s) 388, 390
HindIII AAGCTT 1 cut(s) 801
HinfI GANTC 5 cut(s) 5, 428, 908, 985, 1103
HphI GGTGA 3 cut(s) 193, 574, 1237
Hpy166II GTNNAC 2 cut(s) 103, 511
Hpy188I TCNGA 8 cut(s) 52, 427, 442, 520, 656, 913, 1108, 1201
Hpy188III TCNNGA 4 cut(s) 451, 644, 677, 989
Hpy8I GTNNAC 2 cut(s) 103, 511
Hpy99I CGWCG 1 cut(s) 274
HpyAV CCTTC 3 cut(s) 141, 178, 577
HpyCH4IV ACGT 1 cut(s) 1031
HpyF10VI GCNNNNNNNGC 6 cut(s) 541, 575, 761, 800, 809, 1058
HpyF3I CTNAG 8 cut(s) 159, 360, 449, 519, 545, 579, 910, 1198
HpySE526I ACGT 1 cut(s) 1031
Hsp92II CATG 2 cut(s) 680, 1154
HspAI GCGC 2 cut(s) 388, 390
Kzo9I GATC 4 cut(s) 89, 730, 877, 991
LmnI GCTCC 2 cut(s) 133, 300
Lsp1109I GCAGC 6 cut(s) 159, 325, 465, 646, 748, 1202
LweI GCATC 5 cut(s) 671, 877, 1030, 1177, 1218
MaeI CTAG 1 cut(s) 482
MaeII ACGT 1 cut(s) 1031
MaeIII GTNAC 2 cut(s) 154, 208
MalI GATC 4 cut(s) 91, 732, 879, 993
MboI GATC 4 cut(s) 89, 730, 877, 991
MboII GAAGA 5 cut(s) 152, 466, 497, 622, 1196
MflI RGATCY 2 cut(s) 89, 730
MhlI GDGCHC 2 cut(s) 105, 546
MluCI AATT 6 cut(s) 83, 459, 464, 514, 672, 1142
MlyI GAGTC 2 cut(s) 437, 902
MnlI CCTC 9 cut(s) 46, 49, 120, 154, 232, 442, 1127, 1168, 1199
Mph1103I ATGCAT 1 cut(s) 796
MseI TTAA 4 cut(s) 30, 492, 695, 839
MslI CAYNNNNRTG 1 cut(s) 98
MspA1I CMGCKG 1 cut(s) 1193
MspR9I CCNGG 2 cut(s) 299, 1233
Mva1269I GAATGC 1 cut(s) 571
MvaI CCWGG 2 cut(s) 299, 1233
MvnI CGCG 2 cut(s) 390, 973
MwoI GCNNNNNNNGC 6 cut(s) 541, 575, 761, 800, 809, 1058
NdeII GATC 4 cut(s) 89, 730, 877, 991
NlaIII CATG 2 cut(s) 680, 1154
NlaIV GGNNCC 1 cut(s) 779
NmuCI GTSAC 1 cut(s) 154
NsiI ATGCAT 1 cut(s) 796
NspI RCATGY 1 cut(s) 1154
NspV TTCGAA 1 cut(s) 1240
PagI TCATGA 1 cut(s) 676
PauI GCGCGC 1 cut(s) 388
PciI ACATGT 1 cut(s) 1150
PctI GAATGC 1 cut(s) 571
PfeI GAWTC 3 cut(s) 5, 985, 1103
PflMI CCANNNNNTGG 1 cut(s) 720
PfoI TCCNGGA 1 cut(s) 297
PkrI GCNGC 7 cut(s) 149, 340, 480, 661, 739, 763, 1192
PleI GAGTC 2 cut(s) 436, 902
PpsI GAGTC 2 cut(s) 436, 902
PpuMI RGGWCCY 1 cut(s) 777
PscI ACATGT 1 cut(s) 1150
Psp124BI GAGCTC 1 cut(s) 546
Psp5II RGGWCCY 1 cut(s) 777
Psp6I CCWGG 2 cut(s) 297, 1231
PspFI CCCAGC 1 cut(s) 11
PspGI CCWGG 2 cut(s) 297, 1231
PspN4I GGNNCC 1 cut(s) 779
PspPI GGNCC 3 cut(s) 187, 627, 777
PspPPI RGGWCCY 1 cut(s) 777
PstI CTGCAG 1 cut(s) 343
PsuI RGATCY 2 cut(s) 89, 730
PteI GCGCGC 1 cut(s) 388
PvuII CAGCTG 1 cut(s) 1193
RseI CAYNNNNRTG 1 cut(s) 98
SacI GAGCTC 1 cut(s) 546
SaqAI TTAA 4 cut(s) 30, 492, 695, 839
SatI GCNGC 7 cut(s) 148, 339, 479, 660, 738, 762, 1191
Sau3AI GATC 4 cut(s) 89, 730, 877, 991
Sau96I GGNCC 3 cut(s) 187, 627, 777
SchI GAGTC 2 cut(s) 437, 902
ScrFI CCNGG 2 cut(s) 299, 1233
SduI GDGCHC 2 cut(s) 105, 546
SfaNI GCATC 5 cut(s) 671, 877, 1030, 1177, 1218
SfcI CTRYAG 1 cut(s) 339
SfuI TTCGAA 1 cut(s) 1240
SinI GGWCC 3 cut(s) 187, 627, 777
SmiMI CAYNNNNRTG 1 cut(s) 98
Sse9I AATT 6 cut(s) 83, 459, 464, 514, 672, 1142
SsiI CCGC 2 cut(s) 737, 973
SspI AATATT 1 cut(s) 1047
SspMI CTAG 1 cut(s) 482
SstI GAGCTC 1 cut(s) 546
StyD4I CCNGG 2 cut(s) 297, 1231
TaiI ACGT 1 cut(s) 1034
TaqI TCGA 6 cut(s) 258, 269, 665, 885, 1215, 1240
TasI AATT 6 cut(s) 83, 459, 464, 514, 672, 1142
TauI GCSGC 1 cut(s) 740
TfiI GAWTC 3 cut(s) 5, 985, 1103
Tru1I TTAA 4 cut(s) 30, 492, 695, 839
Tru9I TTAA 4 cut(s) 30, 492, 695, 839
TscAI CASTG 1 cut(s) 579
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 6 cut(s) 147, 338, 478, 659, 761, 1190
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 4 cut(s) 398, 665, 722, 944
TspRI CASTG 1 cut(s) 579
Van91I CCANNNNNTGG 1 cut(s) 720
VneI GTGCAC 1 cut(s) 101
VpaK11BI GGWCC 3 cut(s) 187, 627, 777
XapI RAATTY 1 cut(s) 1142
XceI RCATGY 1 cut(s) 1154
XspI CTAG 1 cut(s) 482
Zsp2I ATGCAT 1 cut(s) 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.