Rroxscaffold_1G00008520

Plant protein of unknown function (DUF641)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011946
Physical Location & Seq
Reverse (-)
10781271 .. 10783961
2691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00008520.1

Sequence Viewer

Length: 1323 bp
ATGGAATCAGCTGGGGCTAAACCCCTTAAACCCTCCTCAAACATATCTGATATTGTCTCCAAGTTTGCCAAAGTTTGCAAATTGAGATCCATTGGAGTGCACCAACCTCACAACAGCAATAATGCTCCTTCGGGTGAAGATGGCAGCGATGTGACTGAGGACACCGAGTGTGGTGGGGTGAAGGTCCATCCCCAACCTGTTGAAGTTACAAGAGAAAGCAAAATGTGTGGAGATGAGGAGTTATCCAAGTTATTCGACATTGTTTCGACGCTGAAGTTGGCTTATGTTCAGCTCCAGGAAGCTCATTTACCCTACAACCCGAAAAAGATTATAGCTGCAGATCAGCGTTTTATGGCTGAGCTTGAAGCAATATGCAAGATGAAGCGCGCGTATAAGGAGAAGCAATTTGTGAAGCTGAAGTCAGATTCCTCTCGTTCTGATATTCTGAGGAAGAAAATTGAATTGAATGAGAAGCTGCTAGAGGAGTTTAAGTTGCAAATGGAAGTCAACAATTCTGAGATAATCTGCCTGCAAAAAGAGCTCAGGGATTTGGATTTGGTGAATGCGACACTGGCTGAGAAGGTAAGGCTGCTAAGTTTGCAAAAGAAGACTGCAAGGGTTTGGACCATTACAAGATTTCAGGATACTTTCAGAGCTGCGTCGAAATCAATTCATGACTTCGCAAAGCCTTTAATCAGCTTGATGAAAGCTACAGGATGGGACTTGGATCTGGCGGCAAAGGCAGTAGAAGCTGAAGCTGCTTACTTGAAAAGGTCGCACAAGAAGTATGCATTTGAAGCTTACATTGCAAGGAGAATGTTTTATGGGTTATCATTGAAATCTTGTAATGTGGATGGTATTATGAGATATGATGATCCCATCGATGCCTTGATTGAGAACCCAGACTCAGATTTTGCCCGATATTGTGGAGAAAAGTATCTACAAGTTGTTCATCCCATGATGGAAACACAGTTCTTTGGAAATCTGGATCACAGGAAGCTTGTATTGAGTGGGAAGCATCCAAGAACGTCATTTTACCAAATATTTGCAAGAATGGCAAGGTGGGTTTGGGTTTTGCACAGCATTGCACCTTTGATAGATTCTGAAACAAAAATGTTTGCTGTCAAAAGAGGAAGCAAATTCTCACATGTTTATATGGAGTCGGTGGTGGAGGATGGGGAAGATGCAGCTGTCTCAGACGGAGGACAGGTCGAGTTTATGGTGATGCCAGGATTTAGAATTTTAGAGACATTAGTGAAGTCTCGTGTGTATCTTTCAAAATGTGGAAGTTGTAATCCGAACTTAGTGATATTTACTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF641

Protein Analysis

440

Amino Acids

49.9

Weight (kDa)

8.53

Isoelectric Point (pI)

42.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF641 PF04859 76 - 194 2e-25 Plant protein of unknown function (DUF641)
GIL1_IRKI_C PF24994 372 - 424 6.2e-20 GIL1/IRKI C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011946)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30380
fragaria_vesca FvH4_3g40290
malus_domestica MD03G1053800.v1.1 MD11G1055400.v1.1
prunus_persica Prupe.6G042900_v2.0.a1 Prupe.6G042900_v2.0.a1 Prupe.6G042900_v2.0.a1 Prupe.6G042900_v2.0.a1
pyrus_communis pycom03g04270
rosa_chinensis RchiOBHm_Chr5g0072631
rosa_laevigata RLG00000036318
rosa_multiflora Rmu_sc0002088.1_g000004
rosa_roxburghii Rroxscaffold_1G00008520
rosa_rugosa Rorug05G0418900
rosa_samantha Rh5AG475800 Rh5BG495900 Rh5CG519700 Rh5DG508400
rosa_wichuraiana Rw5G044180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 387, 389
AciI CCGC 1 cut(s) 734
AclWI GGATC 4 cut(s) 81, 735, 869, 996
AcsI RAATTY 2 cut(s) 1139, 1239
AcuI CTGAAG 3 cut(s) 293, 437, 774
AdeI CACNNNGTG 1 cut(s) 168
AflIII ACRYGT 1 cut(s) 1147
AgsI TTSAA 8 cut(s) 203, 365, 461, 466, 769, 797, 838, 1278
AjnI CCWGG 2 cut(s) 294, 1228
AloI GAACNNNNNNTCC 2 cut(s) 956, 988
Alw21I GWGCWC 2 cut(s) 102, 543
Alw26I GTCTC 4 cut(s) 61, 1198, 1241, 1266
Alw44I GTGCAC 1 cut(s) 98
AlwI GGATC 4 cut(s) 81, 735, 869, 996
ApaLI GTGCAC 1 cut(s) 98
ApeKI GCWGC 7 cut(s) 144, 335, 475, 589, 656, 758, 1187
ApoI RAATTY 2 cut(s) 1139, 1239
ArsI GACNNNNNNTTYG 2 cut(s) 896, 928
AspLEI GCGC 2 cut(s) 387, 389
AspS9I GGNCC 2 cut(s) 184, 624
AsuHPI GGTGA 4 cut(s) 146, 190, 571, 1234
AvaII GGWCC 2 cut(s) 184, 624
BaeGI GKGCMC 1 cut(s) 102
BanII GRGCYC 1 cut(s) 543
BarI GAAGNNNNNNTAC 2 cut(s) 291, 323
BauI CACGAG 1 cut(s) 1263
BbsI GAAGAC 1 cut(s) 614
Bbv12I GWGCWC 2 cut(s) 102, 543
BbvI GCAGC 7 cut(s) 156, 322, 462, 576, 643, 745, 1199
BccI CCATC 7 cut(s) 134, 195, 711, 848, 887, 955, 1169
BciT130I CCWGG 2 cut(s) 296, 1230
BciVI GTATCC 1 cut(s) 637
BcoDI GTCTC 4 cut(s) 61, 1198, 1241, 1266
BfaI CTAG 1 cut(s) 479
BfmI CTRYAG 2 cut(s) 336, 711
BfuI GTATCC 1 cut(s) 637
BisI GCNGC 8 cut(s) 145, 336, 476, 590, 657, 735, 759, 1188
BlpI GCTNAGC 1 cut(s) 357
BlsI GCNGC 8 cut(s) 146, 337, 477, 591, 658, 736, 760, 1189
Bme1390I CCNGG 2 cut(s) 296, 1230
Bme18I GGWCC 2 cut(s) 184, 624
BmgT120I GGNCC 2 cut(s) 184, 624
BmrFI CCNGG 2 cut(s) 296, 1230
BmsI GCATC 4 cut(s) 874, 1027, 1174, 1215
BpiI GAAGAC 1 cut(s) 614
BpmI CTGGAG 1 cut(s) 278
Bpu10I CCTNAGC 1 cut(s) 542
Bpu1102I GCTNAGC 1 cut(s) 357
Bsa29I ATCGAT 1 cut(s) 882
BsaXI ACNNNNNCTCC 4 cut(s) 476, 506, 1194, 1224
Bse1I ACTGG 1 cut(s) 576
Bse3DI GCAATG 2 cut(s) 804, 1083
BseBI CCWGG 2 cut(s) 296, 1230
BseCI ATCGAT 1 cut(s) 882
BseGI GGATG 6 cut(s) 187, 722, 859, 952, 1018, 1180
BseMI GCAATG 2 cut(s) 804, 1083
BseMII CTCAG 8 cut(s) 147, 348, 437, 507, 556, 567, 921, 1209
BseNI ACTGG 1 cut(s) 576
BsePI GCGCGC 1 cut(s) 385
BseRI GAGGAG 3 cut(s) 25, 251, 497
BseSI GKGCMC 1 cut(s) 102
BseXI GCAGC 7 cut(s) 156, 322, 462, 576, 643, 745, 1199
BseYI CCCAGC 1 cut(s) 11
Bsh1236I CGCG 2 cut(s) 387, 389
BshVI ATCGAT 1 cut(s) 882
BsiHKAI GWGCWC 2 cut(s) 102, 543
BslFI GGGAC 1 cut(s) 734
BsmAI GTCTC 4 cut(s) 61, 1198, 1241, 1266
BsmFI GGGAC 1 cut(s) 734
BsmI GAATGC 1 cut(s) 568
Bsp1286I GDGCHC 2 cut(s) 102, 543
Bsp143I GATC 5 cut(s) 86, 340, 727, 874, 988
Bsp1720I GCTNAGC 1 cut(s) 357
BspACI CCGC 1 cut(s) 734
BspCNI CTCAG 8 cut(s) 148, 349, 438, 508, 555, 568, 920, 1208
BspDI ATCGAT 1 cut(s) 882
BspFNI CGCG 2 cut(s) 387, 389
BspHI TCATGA 1 cut(s) 673
BspMAI CTGCAG 1 cut(s) 340
BspPI GGATC 4 cut(s) 81, 735, 869, 996
BsrDI GCAATG 2 cut(s) 804, 1083
BsrI ACTGG 1 cut(s) 576
BssHII GCGCGC 1 cut(s) 385
BssMI GATC 5 cut(s) 86, 340, 727, 874, 988
BssSI CACGAG 1 cut(s) 1263
Bst2BI CACGAG 1 cut(s) 1263
Bst2UI CCWGG 2 cut(s) 296, 1230
Bst4CI ACNGT 1 cut(s) 972
BstC8I GCNNGC 2 cut(s) 387, 530
BstF5I GGATG 6 cut(s) 187, 722, 859, 952, 1018, 1180
BstFNI CGCG 2 cut(s) 387, 389
BstHHI GCGC 2 cut(s) 387, 389
BstKTI GATC 5 cut(s) 89, 343, 730, 877, 991
BstMAI GTCTC 4 cut(s) 61, 1198, 1241, 1266
BstMBI GATC 5 cut(s) 86, 340, 727, 874, 988
BstMWI GCNNNNNNNGC 9 cut(s) 538, 572, 598, 740, 749, 758, 797, 806, 1055
BstNI CCWGG 2 cut(s) 296, 1230
BstNSI RCATGY 1 cut(s) 1151
BstSCI CCNGG 2 cut(s) 294, 1228
BstSFI CTRYAG 2 cut(s) 336, 711
BstSLI GKGCMC 1 cut(s) 102
BstUI CGCG 2 cut(s) 387, 389
BstV1I GCAGC 7 cut(s) 156, 322, 462, 576, 643, 745, 1199
BstV2I GAAGAC 1 cut(s) 614
BstX2I RGATCY 2 cut(s) 86, 727
BstYI RGATCY 2 cut(s) 86, 727
Bsu15I ATCGAT 1 cut(s) 882
BsuI GTATCC 1 cut(s) 637
BsuTUI ATCGAT 1 cut(s) 882
BtgZI GCGATG 1 cut(s) 162
BtsCI GGATG 6 cut(s) 187, 722, 859, 952, 1018, 1180
BtsIMutI CAGTG 1 cut(s) 569
Cac8I GCNNGC 2 cut(s) 387, 530
CciI TCATGA 1 cut(s) 673
CfoI GCGC 2 cut(s) 387, 389
Cfr13I GGNCC 2 cut(s) 184, 624
ClaI ATCGAT 1 cut(s) 882
CseI GACGC 2 cut(s) 277, 648
CspCI CAANNNNNGTGG 2 cut(s) 208, 243
CviAII CATG 3 cut(s) 674, 958, 1148
DpnI GATC 5 cut(s) 88, 342, 729, 876, 990
DpnII GATC 5 cut(s) 86, 340, 727, 874, 988
DraIII CACNNNGTG 1 cut(s) 168
Ecl136II GAGCTC 1 cut(s) 541
Eco24I GRGCYC 1 cut(s) 543
Eco47I GGWCC 2 cut(s) 184, 624
Eco53kI GAGCTC 1 cut(s) 541
Eco57I CTGAAG 3 cut(s) 293, 437, 774
EcoICRI GAGCTC 1 cut(s) 541
EcoRII CCWGG 2 cut(s) 294, 1228
EcoT22I ATGCAT 1 cut(s) 793
EcoT38I GRGCYC 1 cut(s) 543
FaeI CATG 3 cut(s) 677, 961, 1151
FaqI GGGAC 1 cut(s) 734
FatI CATG 3 cut(s) 673, 957, 1147
Fnu4HI GCNGC 8 cut(s) 145, 336, 476, 590, 657, 735, 759, 1188
FokI GGATG 6 cut(s) 174, 729, 866, 939, 1005, 1187
FriOI GRGCYC 1 cut(s) 543
Fsp4HI GCNGC 8 cut(s) 145, 336, 476, 590, 657, 735, 759, 1188
FspBI CTAG 1 cut(s) 479
GlaI GCGC 2 cut(s) 386, 388
GluI GCNGC 8 cut(s) 145, 336, 476, 590, 657, 735, 759, 1188
GsaI CCCAGC 1 cut(s) 15
GsuI CTGGAG 1 cut(s) 278
HgaI GACGC 2 cut(s) 277, 648
HhaI GCGC 2 cut(s) 387, 389
Hin1II CATG 3 cut(s) 677, 961, 1151
Hin6I GCGC 2 cut(s) 385, 387
HinP1I GCGC 2 cut(s) 385, 387
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HindIII AAGCTT 2 cut(s) 798, 998
HinfI GANTC 5 cut(s) 5, 425, 905, 1100, 1160
HphI GGTGA 4 cut(s) 146, 190, 571, 1234
Hpy166II GTNNAC 2 cut(s) 100, 508
Hpy188III TCNNGA 3 cut(s) 641, 674, 986
Hpy8I GTNNAC 2 cut(s) 100, 508
Hpy99I CGWCG 2 cut(s) 271, 664
HpyAV CCTTC 3 cut(s) 138, 175, 574
HpyCH4III ACNGT 1 cut(s) 972
HpyCH4IV ACGT 1 cut(s) 1028
HpyF10VI GCNNNNNNNGC 9 cut(s) 538, 572, 598, 740, 749, 758, 797, 806, 1055
HpySE526I ACGT 1 cut(s) 1028
Hsp92II CATG 3 cut(s) 677, 961, 1151
HspAI GCGC 2 cut(s) 385, 387
Kzo9I GATC 5 cut(s) 86, 340, 727, 874, 988
LmnI GCTCC 2 cut(s) 130, 297
Lsp1109I GCAGC 7 cut(s) 156, 322, 462, 576, 643, 745, 1199
LweI GCATC 4 cut(s) 874, 1027, 1174, 1215
MaeI CTAG 1 cut(s) 479
MaeII ACGT 1 cut(s) 1028
MaeIII GTNAC 2 cut(s) 151, 205
MalI GATC 5 cut(s) 88, 342, 729, 876, 990
MboI GATC 5 cut(s) 86, 340, 727, 874, 988
MboII GAAGA 4 cut(s) 149, 463, 619, 1193
MflI RGATCY 2 cut(s) 86, 727
MhlI GDGCHC 2 cut(s) 102, 543
MluCI AATT 8 cut(s) 80, 404, 456, 461, 511, 669, 1139, 1239
MlyI GAGTC 2 cut(s) 899, 1169
Mph1103I ATGCAT 1 cut(s) 793
MseI TTAA 3 cut(s) 27, 489, 692
MslI CAYNNNNRTG 1 cut(s) 95
MspA1I CMGCKG 2 cut(s) 11, 1190
MspR9I CCNGG 2 cut(s) 296, 1230
Mva1269I GAATGC 1 cut(s) 568
MvaI CCWGG 2 cut(s) 296, 1230
MvnI CGCG 2 cut(s) 387, 389
MwoI GCNNNNNNNGC 9 cut(s) 538, 572, 598, 740, 749, 758, 797, 806, 1055
NdeII GATC 5 cut(s) 86, 340, 727, 874, 988
NlaIII CATG 3 cut(s) 677, 961, 1151
NmuCI GTSAC 1 cut(s) 151
NsiI ATGCAT 1 cut(s) 793
NspI RCATGY 1 cut(s) 1151
PagI TCATGA 1 cut(s) 673
PauI GCGCGC 1 cut(s) 385
PciI ACATGT 1 cut(s) 1147
PctI GAATGC 1 cut(s) 568
PfeI GAWTC 3 cut(s) 5, 425, 1100
PfoI TCCNGGA 1 cut(s) 294
PkrI GCNGC 8 cut(s) 146, 337, 477, 591, 658, 736, 760, 1189
PleI GAGTC 2 cut(s) 899, 1168
PpsI GAGTC 2 cut(s) 899, 1168
PscI ACATGT 1 cut(s) 1147
Psp124BI GAGCTC 1 cut(s) 543
Psp6I CCWGG 2 cut(s) 294, 1228
PspFI CCCAGC 1 cut(s) 11
PspGI CCWGG 2 cut(s) 294, 1228
PspPI GGNCC 2 cut(s) 184, 624
PstI CTGCAG 1 cut(s) 340
PsuI RGATCY 2 cut(s) 86, 727
PteI GCGCGC 1 cut(s) 385
PvuII CAGCTG 2 cut(s) 11, 1190
RseI CAYNNNNRTG 1 cut(s) 95
SacI GAGCTC 1 cut(s) 543
SaqAI TTAA 3 cut(s) 27, 489, 692
SatI GCNGC 8 cut(s) 145, 336, 476, 590, 657, 735, 759, 1188
Sau3AI GATC 5 cut(s) 86, 340, 727, 874, 988
Sau96I GGNCC 2 cut(s) 184, 624
SchI GAGTC 2 cut(s) 899, 1169
ScrFI CCNGG 2 cut(s) 296, 1230
SduI GDGCHC 2 cut(s) 102, 543
SfaNI GCATC 4 cut(s) 874, 1027, 1174, 1215
SfcI CTRYAG 2 cut(s) 336, 711
SinI GGWCC 2 cut(s) 184, 624
SmiMI CAYNNNNRTG 1 cut(s) 95
Sse9I AATT 8 cut(s) 80, 404, 456, 461, 511, 669, 1139, 1239
SsiI CCGC 1 cut(s) 734
SspI AATATT 1 cut(s) 1044
SspMI CTAG 1 cut(s) 479
SstI GAGCTC 1 cut(s) 543
StyD4I CCNGG 2 cut(s) 294, 1228
TaaI ACNGT 1 cut(s) 972
TaiI ACGT 1 cut(s) 1031
TaqI TCGA 5 cut(s) 255, 266, 662, 882, 1212
TasI AATT 8 cut(s) 80, 404, 456, 461, 511, 669, 1139, 1239
TauI GCSGC 1 cut(s) 737
TfiI GAWTC 3 cut(s) 5, 425, 1100
Tru1I TTAA 3 cut(s) 27, 489, 692
Tru9I TTAA 3 cut(s) 27, 489, 692
TscAI CASTG 1 cut(s) 576
TseFI GTSAC 1 cut(s) 151
TseI GCWGC 7 cut(s) 144, 335, 475, 589, 656, 758, 1187
Tsp45I GTSAC 1 cut(s) 151
TspDTI ATGAA 4 cut(s) 395, 662, 719, 941
TspGWI ACGGA 1 cut(s) 1215
TspRI CASTG 1 cut(s) 576
VneI GTGCAC 1 cut(s) 98
VpaK11BI GGWCC 2 cut(s) 184, 624
XapI RAATTY 2 cut(s) 1139, 1239
XceI RCATGY 1 cut(s) 1151
XspI CTAG 1 cut(s) 479
Zsp2I ATGCAT 1 cut(s) 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.