pycom03g04270

Plant protein of unknown function (DUF641)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0011946
Physical Location & Seq
Reverse (-)
3357666 .. 3358991
1326 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g04270.1

Sequence Viewer

Length: 1326 bp
ATGGAATTTACTACTGCCAAACCCCTTAAACCCTCCTCAAACATATCTGATATTGTCTCCAAGTTTGCCAAAGTTTGCAAGTTGAGATCCATTGGTGTGTTTTCGGCCGAAAACTCAGAGCAGAGCCAACAACCCAACAACCCCAAGATTAGAAATGCACATTTGGGTGAGGATAGCAGCGATGTGACGGAGGAGACGGATTGTGATGGGGTGAAAATCCATCCCCATCCCGTGGAAGTTACGAGAACAAGCGATAAGCTTGGGGATGTGGAGTTATCCAAGTTGTTTGACATTGTTTCGGCTTTGAAATTAGCTTATGTTCAGCTTCAGCAAGCTCATTTGCCCTATAACCCGAAGAAGATTGTAGCTGCAGACGAACATTTTATGACTGAGCTTGAAGCGCTCTGCAAGATGAGGAGCGCGTATAAGGAGAAGCAATGTATGAAGCTCAAGTCTGATCCCTCTCGGTTGGATATCCTGAAGAAGAAGGTTGAACTGAATGAGAAGCTACTTGACGAGTTGAAGTTTCAAATGGAAGTTAAGAACTCTGATATACTCTGCGTGCAGAAAGAGCTCGGGGATTTGGCTATGGCGAATGCGGCACTAGCTGAAAAGGTAAAGCAGGTAAGTTTGCAGAGGAAGAATGTAAGGGTGTGGAACATTGCAACATTTCAGGATGCTTTCAGAGCTGCTTCGAAATCCATTCATGATTTTGCGAAGCCATTAATTAGCTTGATGAAAGCTTCAGGCTGGGATTTGGATGTTGCAGCAAAGGCAGTGGAAGTGGAAGCTAGGTATTCGAAAAGGGCCCACAAAAAGTATGCATTTGAAGCCTACATTGCTAAGAGAATGTTTTATGGAATGTCATTGAAATCTTGCAATGTGGATGGAATCATGAGACATGATGACCCTATCGATGCCCTGATAGAGAAACCAGATTCCGACTTTGCCAGATTCTGCGGAGAAAAGTATCTGCTAGTTGTTCATCCAATGATGGAAGCTAGTTTTTTTGGGCATCTGGATAACAGGACACTTGTATTGAGCGGAAAGCATCCGAGGACTTCATTCTACCAAATATTCGCTAGAATGGCAAGGTGCGTTTGGGTGTTACGTGGCATTGCAGCTTCGATAGATTCTGAAGCAAAAATGTTCGCTGTCAAAAGAGGAAGTAAGTTCTCTGATGTCCACATGGAGTCTGTGGAGGAAGACGGGGAAGGTGCAGCTGTGTTGGGTGAACGGCAAGTGGAGTTTATGGTCATGCCCGGATTTAGAATTGGGGAAACAATTGTGAGGTCTCGGGTGTATGTTTCAAAACCAAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF641

Protein Analysis

442

Amino Acids

49.76

Weight (kDa)

8.78

Isoelectric Point (pI)

34.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF641 PF04859 91 - 205 5.5e-26 Plant protein of unknown function (DUF641)
GIL1_IRKI_C PF24994 383 - 435 5.2e-19 GIL1/IRKI C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011946)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30380
fragaria_vesca FvH4_3g40290
malus_domestica MD03G1053800.v1.1 MD11G1055400.v1.1
prunus_persica Prupe.6G042900_v2.0.a1 Prupe.6G042900_v2.0.a1 Prupe.6G042900_v2.0.a1 Prupe.6G042900_v2.0.a1
pyrus_communis pycom03g04270
rosa_chinensis RchiOBHm_Chr5g0072631
rosa_laevigata RLG00000036318
rosa_multiflora Rmu_sc0002088.1_g000004
rosa_roxburghii Rroxscaffold_1G00008520
rosa_rugosa Rorug05G0418900
rosa_samantha Rh5AG475800 Rh5BG495900 Rh5CG519700 Rh5DG508400
rosa_wichuraiana Rw5G044180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 613
AccB7I CCANNNNNTGG 1 cut(s) 232
AccBSI CCGCTC 1 cut(s) 1044
AccII CGCG 1 cut(s) 422
AciI CCGC 3 cut(s) 599, 960, 1044
AclWI GGATC 2 cut(s) 81, 452
AcoI YGGCCR 1 cut(s) 105
AcsI RAATTY 1 cut(s) 5
AcuI CTGAAG 4 cut(s) 311, 500, 729, 1158
AfeI AGCGCT 1 cut(s) 402
AfiI CCNNNNNNNGG 1 cut(s) 232
AgsI TTSAA 8 cut(s) 307, 398, 494, 523, 530, 830, 871, 1311
Alw21I GWGCWC 1 cut(s) 576
Alw26I GTCTC 4 cut(s) 61, 188, 892, 1299
AlwI GGATC 2 cut(s) 81, 452
AlwNI CAGNNNCTG 1 cut(s) 957
Ama87I CYCGRG 2 cut(s) 575, 1296
Aor51HI AGCGCT 1 cut(s) 402
AoxI GGCC 2 cut(s) 105, 807
ApaI GGGCCC 1 cut(s) 811
ApeKI GCWGC 6 cut(s) 177, 368, 689, 767, 1121, 1220
ApoI RAATTY 1 cut(s) 5
AseI ATTAAT 1 cut(s) 725
AspLEI GCGC 2 cut(s) 403, 422
AspS9I GGNCC 2 cut(s) 807, 808
AsuC2I CCSGG 1 cut(s) 1263
AsuHPI GGTGA 3 cut(s) 179, 223, 1244
AsuII TTCGAA 2 cut(s) 695, 800
AvaI CYCGRG 2 cut(s) 575, 1296
BaeGI GKGCMC 1 cut(s) 811
BanII GRGCYC 2 cut(s) 576, 811
BbsI GAAGAC 1 cut(s) 1212
Bbv12I GWGCWC 1 cut(s) 576
BbvI GCAGC 6 cut(s) 189, 355, 676, 779, 1133, 1232
BccI CCATC 5 cut(s) 200, 228, 234, 881, 988
BceAI ACGGC 1 cut(s) 1253
BcnI CCSGG 1 cut(s) 1263
BcoDI GTCTC 4 cut(s) 61, 188, 892, 1299
BfaI CTAG 5 cut(s) 605, 792, 977, 1002, 1083
BfmI CTRYAG 1 cut(s) 369
BfoI RGCGCY 1 cut(s) 404
BfuAI ACCTGC 1 cut(s) 613
BisI GCNGC 7 cut(s) 178, 369, 600, 690, 768, 1122, 1221
BlsI GCNGC 7 cut(s) 179, 370, 601, 691, 769, 1123, 1222
Bme1390I CCNGG 1 cut(s) 1263
BmeT110I CYCGRG 2 cut(s) 575, 1296
BmgT120I GGNCC 2 cut(s) 807, 808
BmiI GGNNCC 1 cut(s) 809
BmrFI CCNGG 1 cut(s) 1263
BmsI GCATC 4 cut(s) 667, 907, 1024, 1060
BpiI GAAGAC 1 cut(s) 1212
Bpu14I TTCGAA 2 cut(s) 695, 800
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 1 cut(s) 1263
Bsa29I ATCGAT 1 cut(s) 915
BsaAI YACGTR 1 cut(s) 1112
BsaI GGTCTC 1 cut(s) 1299
BsaJI CCNNGG 2 cut(s) 231, 1055
Bsc4I CCNNNNNNNGG 1 cut(s) 232
Bse3DI GCAATG 5 cut(s) 443, 660, 837, 886, 1116
BseCI ATCGAT 1 cut(s) 915
BseDI CCNNGG 2 cut(s) 231, 1055
BseGI GGATG 8 cut(s) 220, 226, 271, 682, 766, 892, 985, 1051
BseLI CCNNNNNNNGG 1 cut(s) 232
BseMI GCAATG 5 cut(s) 443, 660, 837, 886, 1116
BseMII CTCAG 2 cut(s) 129, 381
BseRI GAGGAG 3 cut(s) 25, 206, 430
BseSI GKGCMC 1 cut(s) 811
BseX3I CGGCCG 1 cut(s) 105
BseXI GCAGC 6 cut(s) 189, 355, 676, 779, 1133, 1232
BseYI CCCAGC 1 cut(s) 750
BsgI GTGCAG 2 cut(s) 584, 1239
Bsh1236I CGCG 1 cut(s) 422
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 2 cut(s) 107, 809
BshVI ATCGAT 1 cut(s) 915
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 576
BsiHKCI CYCGRG 2 cut(s) 575, 1296
BsiSI CCGG 1 cut(s) 1263
BslI CCNNNNNNNGG 1 cut(s) 232
BsmAI GTCTC 4 cut(s) 61, 188, 892, 1299
BsmBI CGTCTC 1 cut(s) 188
BsmI GAATGC 1 cut(s) 601
BsnI GGCC 2 cut(s) 107, 809
Bso31I GGTCTC 1 cut(s) 1299
BsoBI CYCGRG 2 cut(s) 575, 1296
Bsp119I TTCGAA 2 cut(s) 695, 800
Bsp120I GGGCCC 1 cut(s) 807
Bsp1286I GDGCHC 2 cut(s) 576, 811
Bsp143I GATC 2 cut(s) 86, 457
BspACI CCGC 3 cut(s) 599, 960, 1044
BspANI GGCC 2 cut(s) 107, 809
BspCNI CTCAG 2 cut(s) 128, 382
BspDI ATCGAT 1 cut(s) 915
BspFNI CGCG 1 cut(s) 422
BspHI TCATGA 2 cut(s) 706, 894
BspLI GGNNCC 1 cut(s) 809
BspMAI CTGCAG 1 cut(s) 373
BspMI ACCTGC 1 cut(s) 613
BspPI GGATC 2 cut(s) 81, 452
BspT104I TTCGAA 2 cut(s) 695, 800
BspTNI GGTCTC 1 cut(s) 1299
BsrBI CCGCTC 1 cut(s) 1044
BsrDI GCAATG 5 cut(s) 443, 660, 837, 886, 1116
BssECI CCNNGG 2 cut(s) 231, 1055
BssMI GATC 2 cut(s) 86, 457
BstBAI YACGTR 1 cut(s) 1112
BstBI TTCGAA 2 cut(s) 695, 800
BstC8I GCNNGC 2 cut(s) 333, 563
BstDEI CTNAG 3 cut(s) 115, 390, 843
BstDSI CCRYGG 1 cut(s) 231
BstF5I GGATG 8 cut(s) 220, 226, 271, 682, 766, 892, 985, 1051
BstFNI CGCG 1 cut(s) 422
BstH2I RGCGCY 1 cut(s) 404
BstHHI GCGC 2 cut(s) 403, 422
BstKTI GATC 2 cut(s) 89, 460
BstMAI GTCTC 4 cut(s) 61, 188, 892, 1299
BstMBI GATC 2 cut(s) 86, 457
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 9 cut(s) 400, 571, 599, 605, 686, 773, 830, 839, 1088
BstSCI CCNGG 1 cut(s) 1261
BstSFI CTRYAG 1 cut(s) 369
BstSLI GKGCMC 1 cut(s) 811
BstUI CGCG 1 cut(s) 422
BstV1I GCAGC 6 cut(s) 189, 355, 676, 779, 1133, 1232
BstV2I GAAGAC 1 cut(s) 1212
BstX2I RGATCY 1 cut(s) 86
BstYI RGATCY 1 cut(s) 86
BstZI CGGCCG 1 cut(s) 105
Bsu15I ATCGAT 1 cut(s) 915
BsuRI GGCC 2 cut(s) 107, 809
BsuTUI ATCGAT 1 cut(s) 915
BtgI CCRYGG 1 cut(s) 231
BtgZI GCGATG 1 cut(s) 195
BtsCI GGATG 8 cut(s) 220, 226, 271, 682, 766, 892, 985, 1051
BtsI GCAGTG 1 cut(s) 783
BtsIMutI CAGTG 1 cut(s) 783
BveI ACCTGC 1 cut(s) 613
Cac8I GCNNGC 2 cut(s) 333, 563
CaiI CAGNNNCTG 1 cut(s) 957
CciI TCATGA 2 cut(s) 706, 894
CfoI GCGC 2 cut(s) 403, 422
Cfr13I GGNCC 2 cut(s) 807, 808
ClaI ATCGAT 1 cut(s) 915
CspCI CAANNNNNGTGG 2 cut(s) 759, 794
CviAII CATG 5 cut(s) 707, 895, 902, 1189, 1258
DdeI CTNAG 3 cut(s) 115, 390, 843
DpnI GATC 2 cut(s) 88, 459
DpnII GATC 2 cut(s) 86, 457
EaeI YGGCCR 1 cut(s) 105
EagI CGGCCG 1 cut(s) 105
Ecl136II GAGCTC 1 cut(s) 574
EclXI CGGCCG 1 cut(s) 105
Eco24I GRGCYC 2 cut(s) 576, 811
Eco31I GGTCTC 1 cut(s) 1299
Eco32I GATATC 1 cut(s) 475
Eco47III AGCGCT 1 cut(s) 402
Eco52I CGGCCG 1 cut(s) 105
Eco53kI GAGCTC 1 cut(s) 574
Eco57I CTGAAG 4 cut(s) 311, 500, 729, 1158
Eco88I CYCGRG 2 cut(s) 575, 1296
EcoICRI GAGCTC 1 cut(s) 574
EcoO109I RGGNCCY 1 cut(s) 807
EcoRV GATATC 1 cut(s) 475
EcoT22I ATGCAT 1 cut(s) 826
EcoT38I GRGCYC 2 cut(s) 576, 811
Esp3I CGTCTC 1 cut(s) 188
FaeI CATG 5 cut(s) 710, 898, 905, 1192, 1261
FatI CATG 5 cut(s) 706, 894, 901, 1188, 1257
Fnu4HI GCNGC 7 cut(s) 178, 369, 600, 690, 768, 1122, 1221
FokI GGATG 8 cut(s) 207, 213, 278, 689, 773, 899, 972, 1038
FriOI GRGCYC 2 cut(s) 576, 811
Fsp4HI GCNGC 7 cut(s) 178, 369, 600, 690, 768, 1122, 1221
FspBI CTAG 5 cut(s) 605, 792, 977, 1002, 1083
GlaI GCGC 2 cut(s) 402, 421
GluI GCNGC 7 cut(s) 178, 369, 600, 690, 768, 1122, 1221
GsaI CCCAGC 1 cut(s) 754
HaeII RGCGCY 1 cut(s) 404
HaeIII GGCC 2 cut(s) 107, 809
HapII CCGG 1 cut(s) 1263
HhaI GCGC 2 cut(s) 403, 422
Hin1II CATG 5 cut(s) 710, 898, 905, 1192, 1261
Hin6I GCGC 2 cut(s) 401, 420
HinP1I GCGC 2 cut(s) 401, 420
HindIII AAGCTT 2 cut(s) 257, 741
HinfI GANTC 5 cut(s) 891, 938, 954, 1133, 1193
HpaII CCGG 1 cut(s) 1263
HphI GGTGA 3 cut(s) 179, 223, 1244
Hpy166II GTNNAC 2 cut(s) 1186, 1235
Hpy188I TCNGA 9 cut(s) 49, 118, 457, 550, 686, 943, 1056, 1138, 1180
Hpy188III TCNNGA 5 cut(s) 478, 674, 707, 895, 1019
Hpy8I GTNNAC 2 cut(s) 1186, 1235
HpyAV CCTTC 2 cut(s) 481, 1208
HpyCH4IV ACGT 1 cut(s) 1111
HpyF10VI GCNNNNNNNGC 9 cut(s) 400, 571, 599, 605, 686, 773, 830, 839, 1088
HpyF3I CTNAG 3 cut(s) 115, 390, 843
HpySE526I ACGT 1 cut(s) 1111
Hsp92II CATG 5 cut(s) 710, 898, 905, 1192, 1261
HspAI GCGC 2 cut(s) 401, 420
Kzo9I GATC 2 cut(s) 86, 457
LmnI GCTCC 1 cut(s) 417
Lsp1109I GCAGC 6 cut(s) 189, 355, 676, 779, 1133, 1232
LweI GCATC 4 cut(s) 667, 907, 1024, 1060
MaeI CTAG 5 cut(s) 605, 792, 977, 1002, 1083
MaeII ACGT 1 cut(s) 1111
MaeIII GTNAC 3 cut(s) 184, 238, 1107
MalI GATC 2 cut(s) 88, 459
MbiI CCGCTC 1 cut(s) 1044
MboI GATC 2 cut(s) 86, 457
MboII GAAGA 6 cut(s) 367, 370, 493, 496, 652, 1217
MfeI CAATTG 1 cut(s) 1284
MflI RGATCY 1 cut(s) 86
MhlI GDGCHC 2 cut(s) 576, 811
MluCI AATT 5 cut(s) 5, 308, 726, 1272, 1284
MlyI GAGTC 1 cut(s) 1202
MmeI TCCRAC 2 cut(s) 450, 966
Mph1103I ATGCAT 1 cut(s) 826
MseI TTAA 3 cut(s) 27, 540, 725
MslI CAYNNNNRTG 2 cut(s) 95, 165
MspA1I CMGCKG 1 cut(s) 1223
MspI CCGG 1 cut(s) 1263
MspR9I CCNGG 1 cut(s) 1263
MunI CAATTG 1 cut(s) 1284
Mva1269I GAATGC 1 cut(s) 601
MvnI CGCG 1 cut(s) 422
MwoI GCNNNNNNNGC 9 cut(s) 400, 571, 599, 605, 686, 773, 830, 839, 1088
NciI CCSGG 1 cut(s) 1263
NdeII GATC 2 cut(s) 86, 457
NlaIII CATG 5 cut(s) 710, 898, 905, 1192, 1261
NlaIV GGNNCC 1 cut(s) 809
NmuCI GTSAC 1 cut(s) 184
NsiI ATGCAT 1 cut(s) 826
NspV TTCGAA 2 cut(s) 695, 800
PagI TCATGA 2 cut(s) 706, 894
PctI GAATGC 1 cut(s) 601
PfeI GAWTC 4 cut(s) 891, 938, 954, 1133
PflMI CCANNNNNTGG 1 cut(s) 232
PkrI GCNGC 7 cut(s) 179, 370, 601, 691, 769, 1123, 1222
PleI GAGTC 1 cut(s) 1201
PpsI GAGTC 1 cut(s) 1201
Ppu21I YACGTR 1 cut(s) 1112
PshBI ATTAAT 1 cut(s) 725
Psp124BI GAGCTC 1 cut(s) 576
PspFI CCCAGC 1 cut(s) 750
PspN4I GGNNCC 1 cut(s) 809
PspOMI GGGCCC 1 cut(s) 807
PspPI GGNCC 2 cut(s) 807, 808
PstI CTGCAG 1 cut(s) 373
PstNI CAGNNNCTG 1 cut(s) 957
PsuI RGATCY 1 cut(s) 86
PvuII CAGCTG 1 cut(s) 1223
RseI CAYNNNNRTG 2 cut(s) 95, 165
SacI GAGCTC 1 cut(s) 576
SaqAI TTAA 3 cut(s) 27, 540, 725
SatI GCNGC 7 cut(s) 178, 369, 600, 690, 768, 1122, 1221
Sau3AI GATC 2 cut(s) 86, 457
Sau96I GGNCC 2 cut(s) 807, 808
SchI GAGTC 1 cut(s) 1202
ScrFI CCNGG 1 cut(s) 1263
SduI GDGCHC 2 cut(s) 576, 811
SfaNI GCATC 4 cut(s) 667, 907, 1024, 1060
SfcI CTRYAG 1 cut(s) 369
SfuI TTCGAA 2 cut(s) 695, 800
SmiMI CAYNNNNRTG 2 cut(s) 95, 165
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 5 cut(s) 5, 308, 726, 1272, 1284
SsiI CCGC 3 cut(s) 599, 960, 1044
SspI AATATT 1 cut(s) 1077
SspMI CTAG 5 cut(s) 605, 792, 977, 1002, 1083
SstI GAGCTC 1 cut(s) 576
StyD4I CCNGG 1 cut(s) 1261
TaiI ACGT 1 cut(s) 1114
TaqI TCGA 4 cut(s) 695, 800, 915, 1127
TasI AATT 5 cut(s) 5, 308, 726, 1272, 1284
TauI GCSGC 1 cut(s) 602
TfiI GAWTC 4 cut(s) 891, 938, 954, 1133
Tru1I TTAA 3 cut(s) 27, 540, 725
Tru9I TTAA 3 cut(s) 27, 540, 725
TscAI CASTG 1 cut(s) 783
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 6 cut(s) 177, 368, 689, 767, 1121, 1220
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 5 cut(s) 458, 695, 752, 974, 1053
TspGWI ACGGA 2 cut(s) 203, 212
TspRI CASTG 1 cut(s) 783
Van91I CCANNNNNTGG 1 cut(s) 232
VspI ATTAAT 1 cut(s) 725
XapI RAATTY 1 cut(s) 5
XspI CTAG 5 cut(s) 605, 792, 977, 1002, 1083
Zsp2I ATGCAT 1 cut(s) 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.