Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0010977

Physical Location & Seq

Genomic Coordinates Reverse (-)

4950692 .. 4958877

Gene Structure 8186 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1058300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3030 bp

ATGGCGTCCAAGTTCGGGTTGGCCGGAGGCATACCGGAGCGCCGGGTCCGACCCATTTGGGACGCCATCGATTCTCGCCAATTCAAGAACGCTCTCAAGCTCGTCACCGCCCTCCTCTCCAAGTACCCCAATTCGCCTTACTGCCTGGCGCTTAAAGCTCTCGTCCTGGAGAGGATGGGGAAATCCGACGAGGCATTGTCGGTTTGCCTGAGCGCCAAGGAGCTTCTGCACTCAAACGACTCCGTTTTGATGGACGATCTCACTCTCAGCACCTTGCAAATCGTCTTCCAACGGCTCGATCACTTGGAAATGGCGACCAGCTGTTACGAATACGCTTGCGGGAAGTTTTCCAACAATTTGGAGCTCATGATGGGCCTCTTCAACTGCTATGTTCGCGAGTACTCATTCGTAAAGCAGCAACAGACAGCTATAAAAATGTACAAGCTTGTTGGGGAGGAGAGGTTTTTGTTATGGGCAGTGTGTAGCATCCAGTTACAGGTTTTTTGCGGCAATGGGAGTGAGAAGCTTTTAACACTAGCTGAAGGTTTGGTTAAGAAGCATGTTGCCTCCCACAGCTTGCATGAACCTGAAGCTCTTATGGTCTATGTTTCCATATTGGAACAACAAGCCAAATATGGGGATGCTTTGGAAATTCTCTCTGGAAAATTAGGATCGCTTTTGATGGTTGAAGTTGACAAGCTACGCATACAGGGGAGGCTTCATGCTCGGGCAGGTGACTACACTGCTGCTGCCGGCATATTTCAAAGAATCCTAGAATTATGTCCTGATGATTGGGATTGTTTCCTACATTACCTCGGTTGTTTGCTGGAGGATGACAGCAATTGGTGCAATATGGCCATCACAGATCCAATTCATCCTCCAAAATTCATTGAGTGCAAGATTTCAACCTTGGGAGATGAAGTGTTTGATTCTCGCATGTCAAGTGCATCAGATTTTGTAAAGAAGTTACTAGAAAATATTGGTGATAATTTTTTAAGGTCTCCATTCTTGGCAAATATTGAAATTGAAAGGAGGAGGCACTTACATGGCAAAGGGGATGACGACAAGTTAATGGAGTCTCTAATCCAATACTTTATTAGGTTTGGTCACTTGGCCTGCTTCACTTCTGATGTCGAGATGTTTCTTGAAGTTTTAACCCTTGATAAGAAGGCAGAACTGCTGGGGAAGTTAAAGGAAAGCTCTGACTCCACTTCAGCTGTTCCAACAAAAGTTCTTGGGCAGTCAATAACATTCTTTAAAATTCAAGAATTGATTGGAAACACATTCAAGCTTCCTGTGGGTGAACTTGAGGGATCTGCTGTGCAGATGGTCGAGATGTATTGTCAAAACCTTCCACTTTCGAAGGACTTGGATTCACAAGAGAGCATGCATGGGGAGGAGCTGCTCTCAATGGCATGCAATGCGTTGATTCAGTTATTCTGGCGTACAAGGAATTTCGGCTACTATTTTGAAGCAATTATGGTATTGGAGTTCGGCTTGACAATTCGAAAATATGTATGGCAGTACAAAATATTGTTGCTGCACTTGTACTCTCACTTAGGTGCCCTTTCCTTGGCGTATGAGTGGTTCAAGTCGTTGGATGTGAAAAATATCTTGATGGAAACTGTTTCACACCATATTTTACCCCAGATGTTGGTGTCTCCCCTATGGGCAGATTTAAACTATCTGCTCAAGGATTATCTCAGGTTTATGGATGACCACTTAAGAGAATCAGCAGACCTTACATTTCTTGCCTATCGTCATAGAAATTACTCAAAAGTAATTGAATTTGTCCAGTTTAAAGAAAGACTGCAACACTCTAATCAGTATCTTGTGGCGAAGGTTGAAGGACCCATTCTACAGTTAAAGCAGAATGCAGATAACATTGATGATGAAGAGACTGTTCTTGAAAGCTTGAAATGTGGGGTTCACTTTGCCGAACTCTCTAATGAGATTGGATCCAAATCTTTGACCTTCAATGAAGATTTGCAGTCTCGTCCATGGTGGGCACCAACTTCAGAAAGAAACTATCTTTTAGGTCCTTTTGAAGGAGTATCCTATTGTCCGAAAGAGCACTCGGTGAAAGAAAGGGAAGCAAATGTAAGGAGAGTTATTGAGAGGAAGTCTCTTCTTCCTCGGATGATTTATCTATCCATACAGAATGCTTCGACTTCACTCAAAGAAAATCTCGAGGCCAATGGAAACACATCTGACCCTAAAGGCCCCTCAGAACTTAAGAGTTTGCTTGAGCGCTATGCAAAAATGTTGGGGTTCACACTTAATGATGCTATAGAAGCGGTTTTGGGGGTCTCTAGTGGCCTAAAGTCGTTTGAGGTCTTTGGTGCGGACTTGATTGACTGGATAAATTTTTCTGTGTTTCTGAACGCATGGAACTTGAGTTCCCATGAAATTGGTCAAGCAAACGGTGATGCGGGTCTGTCTCGAGCATGGCACTGTGTGGATTCGCTGTTGGAGAAGTATGTTTCGGAGAAAGTTAGCTCGATGGAGACTCTAATTAGCTGTCCTTGGGTTGATGTCCCAGTTCTGGTGCAGTTGGTTACAGAACCGTTGGCCTGGCATGCACTTGTAATTCAATCTTGCACTCGGTCATCTCTTCCGTCTGGAAAGAAGAAGAAGAAAACTGGAGTTCCAGATCATTCTATTCTGTCCCATATGCGGGATTCAGTCCAGTCTCTGCGTAATACTCTGGAAAAAGTTATGAAATGGTTAAGAGAACAGATCAACAGGCCAGAGGATGAAAGTTTGGAGACATTACTCTCCTCTTTGCAGAATAAAGGACAGAACGAAGGCCCTGGGCAAGTTTTCCATATTTTAGAAACCTGTATCTCATCCGTAAATGACACAGAGGTCGGTGATAGAATCTCCCGAGCATTGAAGTCTTGGAGTCCAACTGATGTCGCGCGAAAGCTGATAACTGGGAAATGTACGGTGCTGTCTGAGTTTCTACGGATCTGTGAATCCAAACTAAAGCTGTTGCAGACATTGAAACAGCAGATAGCTCAAGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005829 GO:0005911 GO:0006464 GO:0006473 GO:0006474 GO:0006807 GO:0006810 GO:0006897 GO:0006909 GO:0006911 GO:0006955 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0010324 GO:0010467 GO:0016043 GO:0016192 GO:0017196 GO:0018193 GO:0018206 GO:0019538 GO:0030054 GO:0031248 GO:0031365 GO:0031414 GO:0031416 GO:0032991 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043543 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0051604 GO:0055044 GO:0061024 GO:0071704 GO:0071840 GO:0098657 GO:0099024 GO:1901564 GO:1902493 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NatB_MDM20

Protein Analysis

1010

Amino Acids

114.43

Weight (kDa)

5.9

Isoelectric Point (pI)

43.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NatB_MDM20	PF09797	307 - 631	8.6e-56	N-acetyltransferase B complex (NatB) non catalytic subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010977)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58450 AT5G58450
fragaria_vesca	FvH4_3g39760
malus_domestica	MD03G1056500.v1.1 MD11G1058300.v1.1
prunus_persica	Prupe.6G048400_v2.0.a1
pyrus_communis	pycom03g04480 pycom11g04830
rosa_chinensis	RchiOBHm_Chr5g0071641
rosa_laevigata	RLG00000036235
rosa_multiflora	Rmu_sc0005745.1_g000032
rosa_roxburghii	Rroxscaffold_1G00009550
rosa_rugosa	Rorug05G0413800 Rorug05G0413800 Rorug05G0413800
rosa_samantha	Rh5AG469800 Rh5BG489100 Rh5CG513100 Rh5DG499700
rosa_wichuraiana	Rw5G043650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	722
AasI	GACNNNNNNGTC	1 cut(s)	2867
Acc36I	ACCTGC	1 cut(s)	722
AccB1I	GGYRCC	2 cut(s)	1562, 2008
AccB7I	CCANNNNNTGG	1 cut(s)	1654
AccII	CGCG	3 cut(s)	396, 2921, 2923
AciI	CCGC	7 cut(s)	108, 339, 507, 2297, 2345, 2432, 2677
AclWI	GGATC	6 cut(s)	679, 860, 1321, 1953, 1966, 2978
AcoI	YGGCCR	2 cut(s)	21, 855
AcsI	RAATTY	6 cut(s)	651, 884, 1260, 1453, 1787, 2365
AcuI	CTGAAG	4 cut(s)	561, 609, 1197, 2001
AcyI	GRCGYC	2 cut(s)	5, 63
AdeI	CACNNNGTG	2 cut(s)	2080, 2458
AfaI	GTAC	7 cut(s)	125, 401, 440, 1447, 1526, 1550, 2947
AfeI	AGCGCT	1 cut(s)	2252
AfiI	CCNNNNNNNGG	9 cut(s)	15, 496, 636, 1573, 1654, 2048, 2545, 2676, 2677
AflII	CTTAAG	2 cut(s)	1723, 2234
AjnI	CCWGG	4 cut(s)	144, 165, 2573, 2812
AleI	CACNNNNGTG	1 cut(s)	1560
AloI	GAACNNNNNNTCC	4 cut(s)	2383, 2384, 2415, 2416
Alw21I	GWGCWC	2 cut(s)	366, 2076
AlwI	GGATC	6 cut(s)	679, 860, 1321, 1953, 1966, 2978
AlwNI	CAGNNNCTG	1 cut(s)	2695
Ama87I	CYCGRG	4 cut(s)	726, 2189, 2442, 2886
Aor51HI	AGCGCT	1 cut(s)	2252
ApeKI	GCWGC	5 cut(s)	415, 746, 749, 1402, 1540
ApoI	RAATTY	6 cut(s)	651, 884, 1260, 1453, 1787, 2365
AspLEI	GCGC	5 cut(s)	42, 151, 215, 2253, 2923
AspS9I	GGNCC	6 cut(s)	46, 373, 1850, 2039, 2222, 2810
AsuC2I	CCSGG	1 cut(s)	44
AsuHPI	GGTGA	7 cut(s)	97, 746, 995, 1313, 2092, 2438, 2885
AsuII	TTCGAA	2 cut(s)	1361, 1507
AvaI	CYCGRG	4 cut(s)	726, 2189, 2442, 2886
AvaII	GGWCC	3 cut(s)	46, 1850, 2039
BaeGI	GKGCMC	2 cut(s)	1567, 2011
BalI	TGGCCA	1 cut(s)	857
BamHI	GGATCC	1 cut(s)	1958
BanI	GGYRCC	2 cut(s)	1562, 2008
BanII	GRGCYC	1 cut(s)	366
BbsI	GAAGAC	1 cut(s)	277
Bbv12I	GWGCWC	2 cut(s)	366, 2076
BbvI	GCAGC	5 cut(s)	427, 733, 736, 1389, 1527
BccI	CCATC	9 cut(s)	74, 169, 244, 364, 676, 866, 1321, 1612, 2497
BceAI	ACGGC	1 cut(s)	308
BciT130I	CCWGG	4 cut(s)	146, 167, 2575, 2814
BciVI	GTATCC	1 cut(s)	2065
BcnI	CCSGG	1 cut(s)	44
BfaI	CTAG	4 cut(s)	536, 773, 971, 2313
BfmI	CTRYAG	2 cut(s)	1859, 2289
BfoI	RGCGCY	4 cut(s)	43, 152, 216, 2254
BfrI	CTTAAG	2 cut(s)	1723, 2234
BfuAI	ACCTGC	1 cut(s)	722
BfuI	GTATCC	1 cut(s)	2065
BisI	GCNGC	6 cut(s)	416, 508, 747, 750, 1403, 1541
BlsI	GCNGC	6 cut(s)	417, 509, 748, 751, 1404, 1542
BmcAI	AGTACT	1 cut(s)	401
Bme1390I	CCNGG	5 cut(s)	44, 146, 167, 2575, 2814
Bme18I	GGWCC	3 cut(s)	46, 1850, 2039
BmeT110I	CYCGRG	4 cut(s)	726, 2189, 2442, 2886
BmgT120I	GGNCC	6 cut(s)	46, 373, 1850, 2039, 2222, 2810
BmiI	GGNNCC	6 cut(s)	47, 1564, 1852, 1960, 2010, 2224
BmrFI	CCNGG	5 cut(s)	44, 146, 167, 2575, 2814
BmrI	ACTGGG	2 cut(s)	2534, 2946
BmsI	GCATC	5 cut(s)	495, 631, 956, 2275, 2419
BmuI	ACTGGG	2 cut(s)	2534, 2946
BpiI	GAAGAC	1 cut(s)	277
BplI	GAGNNNNNCTC	4 cut(s)	1391, 1423, 2110, 2142
BpmI	CTGGAG	3 cut(s)	188, 848, 2664
Bpu10I	CCTNAGC	1 cut(s)	209
Bpu14I	TTCGAA	2 cut(s)	1361, 1507
BpuEI	CTTGAG	6 cut(s)	80, 1328, 1676, 2267, 2416, 3006
BpuMI	CCSGG	1 cut(s)	44
Bsa29I	ATCGAT	1 cut(s)	69
BsaBI	GATNNNNATC	1 cut(s)	1963
BsaHI	GRCGYC	2 cut(s)	5, 63
BsaI	GGTCTC	2 cut(s)	1005, 2314
BsaJI	CCNNGG	9 cut(s)	216, 814, 909, 1572, 2000, 2135, 2525, 2812, 2813
BsaWI	WCCGGW	1 cut(s)	34
BsaXI	ACNNNNNCTCC	4 cut(s)	446, 476, 906, 936
Bsc4I	CCNNNNNNNGG	9 cut(s)	15, 496, 636, 1573, 1654, 2048, 2545, 2676, 2677
Bse118I	RCCGGY	1 cut(s)	752
Bse1I	ACTGG	7 cut(s)	490, 1795, 2363, 2540, 2647, 2689, 2941
Bse3DI	GCAATG	2 cut(s)	517, 1426
Bse8I	GATNNNNATC	1 cut(s)	1963
BseBI	CCWGG	4 cut(s)	146, 167, 2575, 2814
BseCI	ATCGAT	1 cut(s)	69
BseDI	CCNNGG	9 cut(s)	216, 814, 909, 1572, 2000, 2135, 2525, 2812, 2813
BseJI	GATNNNNATC	1 cut(s)	1963
BseLI	CCNNNNNNNGG	9 cut(s)	15, 496, 636, 1573, 1654, 2048, 2545, 2676, 2677
BseMI	GCAATG	2 cut(s)	517, 1426
BseMII	CTCAG	5 cut(s)	200, 280, 1717, 2241, 2949
BseNI	ACTGG	7 cut(s)	490, 1795, 2363, 2540, 2647, 2689, 2941
BseRI	GAGGAG	5 cut(s)	104, 470, 1048, 1412, 2770
BseSI	GKGCMC	2 cut(s)	1567, 2011
BseXI	GCAGC	5 cut(s)	427, 733, 736, 1389, 1527
BseYI	CCCAGC	1 cut(s)	1180
BsgI	GTGCAG	4 cut(s)	212, 1343, 1526, 2570
Bsh1236I	CGCG	3 cut(s)	396, 2921, 2923
BshNI	GGYRCC	2 cut(s)	1562, 2008
BshVI	ATCGAT	1 cut(s)	69
BsiHKAI	GWGCWC	2 cut(s)	366, 2076
BsiHKCI	CYCGRG	4 cut(s)	726, 2189, 2442, 2886
BsiSI	CCGG	4 cut(s)	24, 35, 43, 753
BslFI	GGGAC	3 cut(s)	74, 2522, 2653
BslI	CCNNNNNNNGG	9 cut(s)	15, 496, 636, 1573, 1654, 2048, 2545, 2676, 2677
BsmFI	GGGAC	3 cut(s)	74, 2522, 2653
BsmI	GAATGC	2 cut(s)	1879, 2167
Bso31I	GGTCTC	2 cut(s)	1005, 2314
BsoBI	CYCGRG	4 cut(s)	726, 2189, 2442, 2886
Bsp119I	TTCGAA	2 cut(s)	1361, 1507
Bsp1286I	GDGCHC	4 cut(s)	366, 1567, 2011, 2076
Bsp1407I	TGTACA	1 cut(s)	438
Bsp143I	GATC	9 cut(s)	256, 298, 671, 865, 1313, 1958, 2653, 2739, 2970
Bsp19I	CCATGG	1 cut(s)	2000
Bsp68I	TCGCGA	1 cut(s)	396
BspACI	CCGC	7 cut(s)	108, 339, 507, 2297, 2345, 2432, 2677
BspCNI	CTCAG	5 cut(s)	201, 279, 1716, 2240, 2950
BspDI	ATCGAT	1 cut(s)	69
BspFNI	CGCG	3 cut(s)	396, 2921, 2923
BspHI	TCATGA	1 cut(s)	366
BspLI	GGNNCC	6 cut(s)	47, 1564, 1852, 1960, 2010, 2224
BspMI	ACCTGC	1 cut(s)	722
BspPI	GGATC	6 cut(s)	679, 860, 1321, 1953, 1966, 2978
BspT104I	TTCGAA	2 cut(s)	1361, 1507
BspT107I	GGYRCC	2 cut(s)	1562, 2008
BspTI	CTTAAG	2 cut(s)	1723, 2234
BspTNI	GGTCTC	2 cut(s)	1005, 2314
BsrDI	GCAATG	2 cut(s)	517, 1426
BsrFI	RCCGGY	1 cut(s)	752
BsrGI	TGTACA	1 cut(s)	438
BsrI	ACTGG	7 cut(s)	490, 1795, 2363, 2540, 2647, 2689, 2941
BssAI	RCCGGY	1 cut(s)	752
BssECI	CCNNGG	9 cut(s)	216, 814, 909, 1572, 2000, 2135, 2525, 2812, 2813
BssMI	GATC	9 cut(s)	256, 298, 671, 865, 1313, 1958, 2653, 2739, 2970
BssNI	GRCGYC	2 cut(s)	5, 63
BssT1I	CCWWGG	5 cut(s)	216, 909, 1572, 2000, 2525
Bst2UI	CCWGG	4 cut(s)	146, 167, 2575, 2814
Bst4CI	ACNGT	7 cut(s)	1627, 1863, 1903, 2426, 2456, 2568, 2950
Bst6I	CTCTTC	4 cut(s)	383, 1890, 2133, 2619
BstACI	GRCGYC	2 cut(s)	5, 63
BstAFI	CTTAAG	2 cut(s)	1723, 2234
BstAPI	GCANNNNNTGC	2 cut(s)	846, 1421
BstAUI	TGTACA	1 cut(s)	438
BstBI	TTCGAA	2 cut(s)	1361, 1507
BstC8I	GCNNGC	7 cut(s)	337, 578, 754, 1117, 1388, 1417, 2580
BstDEI	CTNAG	6 cut(s)	209, 266, 1558, 1703, 2227, 2958
BstDSI	CCRYGG	1 cut(s)	2000
BstENI	CCTNNNNNAGG	1 cut(s)	2046
BstFNI	CGCG	3 cut(s)	396, 2921, 2923
BstH2I	RGCGCY	4 cut(s)	43, 152, 216, 2254
BstHHI	GCGC	5 cut(s)	42, 151, 215, 2253, 2923
BstKTI	GATC	9 cut(s)	259, 301, 674, 868, 1316, 1961, 2656, 2742, 2973
BstMBI	GATC	9 cut(s)	256, 298, 671, 865, 1313, 1958, 2653, 2739, 2970
BstMWI	GCNNNNNNNGC	6 cut(s)	155, 393, 846, 1421, 2294, 2579
BstNI	CCWGG	4 cut(s)	146, 167, 2575, 2814
BstNSI	RCATGY	5 cut(s)	563, 940, 1390, 1419, 2582
BstSCI	CCNGG	5 cut(s)	42, 144, 165, 2573, 2812
BstSFI	CTRYAG	2 cut(s)	1859, 2289
BstSLI	GKGCMC	2 cut(s)	1567, 2011
BstUI	CGCG	3 cut(s)	396, 2921, 2923
BstV1I	GCAGC	5 cut(s)	427, 733, 736, 1389, 1527
BstV2I	GAAGAC	1 cut(s)	277
BstX2I	RGATCY	4 cut(s)	865, 1313, 1958, 2970
BstXI	CCANNNNNNTGG	2 cut(s)	358, 2411
BstYI	RGATCY	4 cut(s)	865, 1313, 1958, 2970
Bsu15I	ATCGAT	1 cut(s)	69
BsuI	GTATCC	1 cut(s)	2065
BsuTUI	ATCGAT	1 cut(s)	69
BtgI	CCRYGG	1 cut(s)	2000
BtsI	GCAGTG	2 cut(s)	483, 741
BtsIMutI	CAGTG	3 cut(s)	483, 741, 2452
BtuMI	TCGCGA	1 cut(s)	396
BveI	ACCTGC	1 cut(s)	722
Cac8I	GCNNGC	7 cut(s)	337, 578, 754, 1117, 1388, 1417, 2580
CaiI	CAGNNNCTG	1 cut(s)	2695
CciI	TCATGA	1 cut(s)	366
CfoI	GCGC	5 cut(s)	42, 151, 215, 2253, 2923
Cfr10I	RCCGGY	1 cut(s)	752
Cfr13I	GGNCC	6 cut(s)	46, 373, 1850, 2039, 2222, 2810
ClaI	ATCGAT	1 cut(s)	69
CseI	GACGC	1 cut(s)	71
Csp6I	GTAC	7 cut(s)	124, 400, 439, 1446, 1525, 1549, 2946
CviQI	GTAC	7 cut(s)	124, 400, 439, 1446, 1525, 1549, 2946
DdeI	CTNAG	6 cut(s)	209, 266, 1558, 1703, 2227, 2958
DpnI	GATC	9 cut(s)	258, 300, 673, 867, 1315, 1960, 2655, 2741, 2972
DpnII	GATC	9 cut(s)	256, 298, 671, 865, 1313, 1958, 2653, 2739, 2970
DraI	TTTAAA	3 cut(s)	1258, 1680, 1801
DraIII	CACNNNGTG	2 cut(s)	2080, 2458
DrdI	GACNNNNNNGTC	1 cut(s)	2867
DseDI	GACNNNNNNGTC	1 cut(s)	2867
EaeI	YGGCCR	2 cut(s)	21, 855
Eam1104I	CTCTTC	4 cut(s)	383, 1890, 2133, 2619
EarI	CTCTTC	4 cut(s)	383, 1890, 2133, 2619
Ecl136II	GAGCTC	1 cut(s)	364
Eco130I	CCWWGG	5 cut(s)	216, 909, 1572, 2000, 2525
Eco24I	GRGCYC	1 cut(s)	366
Eco31I	GGTCTC	2 cut(s)	1005, 2314
Eco47I	GGWCC	3 cut(s)	46, 1850, 2039
Eco47III	AGCGCT	1 cut(s)	2252
Eco53kI	GAGCTC	1 cut(s)	364
Eco57I	CTGAAG	4 cut(s)	561, 609, 1197, 2001
Eco88I	CYCGRG	4 cut(s)	726, 2189, 2442, 2886
EcoICRI	GAGCTC	1 cut(s)	364
EcoNI	CCTNNNNNAGG	1 cut(s)	2046
EcoO109I	RGGNCCY	4 cut(s)	1850, 2039, 2222, 2810
EcoRII	CCWGG	4 cut(s)	144, 165, 2573, 2812
EcoT14I	CCWWGG	5 cut(s)	216, 909, 1572, 2000, 2525
EcoT22I	ATGCAT	1 cut(s)	1392
EcoT38I	GRGCYC	1 cut(s)	366
ErhI	CCWWGG	5 cut(s)	216, 909, 1572, 2000, 2525
FaqI	GGGAC	3 cut(s)	74, 2522, 2653
FauI	CCCGC	3 cut(s)	332, 2425, 2670
FauNDI	CATATG	1 cut(s)	2673
Fnu4HI	GCNGC	6 cut(s)	416, 508, 747, 750, 1403, 1541
FriOI	GRGCYC	1 cut(s)	366
Fsp4HI	GCNGC	6 cut(s)	416, 508, 747, 750, 1403, 1541
FspBI	CTAG	4 cut(s)	536, 773, 971, 2313
GlaI	GCGC	5 cut(s)	41, 150, 214, 2252, 2922
GluI	GCNGC	6 cut(s)	416, 508, 747, 750, 1403, 1541
GsaI	CCCAGC	1 cut(s)	1184
GsuI	CTGGAG	3 cut(s)	188, 848, 2664
HaeII	RGCGCY	4 cut(s)	43, 152, 216, 2254
HapII	CCGG	4 cut(s)	24, 35, 43, 753
HgaI	GACGC	1 cut(s)	71
HhaI	GCGC	5 cut(s)	42, 151, 215, 2253, 2923
Hin1I	GRCGYC	2 cut(s)	5, 63
Hin6I	GCGC	5 cut(s)	40, 149, 213, 2251, 2921
HinP1I	GCGC	5 cut(s)	40, 149, 213, 2251, 2921
HincII	GTYRAC	1 cut(s)	694
HindII	GTYRAC	1 cut(s)	694
HindIII	AAGCTT	4 cut(s)	443, 524, 1289, 1912
HpaII	CCGG	4 cut(s)	24, 35, 43, 753
HphI	GGTGA	7 cut(s)	97, 746, 995, 1313, 2092, 2438, 2885
Hpy166II	GTNNAC	4 cut(s)	694, 1304, 1930, 2274
Hpy8I	GTNNAC	4 cut(s)	694, 1304, 1930, 2274
Hpy99I	CGWCG	1 cut(s)	191
HpyAV	CCTTC	9 cut(s)	536, 1162, 1357, 1361, 1834, 1841, 1984, 2042, 2801
HpyCH4III	ACNGT	7 cut(s)	1627, 1863, 1903, 2426, 2456, 2568, 2950
HpyF10VI	GCNNNNNNNGC	6 cut(s)	155, 393, 846, 1421, 2294, 2579
HpyF3I	CTNAG	6 cut(s)	209, 266, 1558, 1703, 2227, 2958
Hsp92I	GRCGYC	2 cut(s)	5, 63
HspAI	GCGC	5 cut(s)	40, 149, 213, 2251, 2921
KroI	GCCGGC	1 cut(s)	752
KroNI	GCCGGC	1 cut(s)	754
Kzo9I	GATC	9 cut(s)	256, 298, 671, 865, 1313, 1958, 2653, 2739, 2970
LmnI	GCTCC	4 cut(s)	37, 220, 361, 1399
Lsp1109I	GCAGC	5 cut(s)	427, 733, 736, 1389, 1527
LweI	GCATC	5 cut(s)	495, 631, 956, 2275, 2419
MaeI	CTAG	4 cut(s)	536, 773, 971, 2313
MaeIII	GTNAC	7 cut(s)	103, 323, 492, 734, 966, 1106, 2557
MalI	GATC	9 cut(s)	258, 300, 673, 867, 1315, 1960, 2655, 2741, 2972
MboI	GATC	9 cut(s)	256, 298, 671, 865, 1313, 1958, 2653, 2739, 2970
MfeI	CAATTG	1 cut(s)	841
MflI	RGATCY	4 cut(s)	865, 1313, 1958, 2970
MhlI	GDGCHC	4 cut(s)	366, 1567, 2011, 2076
MlsI	TGGCCA	1 cut(s)	857
MluNI	TGGCCA	1 cut(s)	857
MlyI	GAGTC	5 cut(s)	233, 1085, 1199, 2503, 2914
MmeI	TCCRAC	8 cut(s)	73, 210, 313, 375, 1247, 1578, 2451, 2933
Mox20I	TGGCCA	1 cut(s)	857
Mph1103I	ATGCAT	1 cut(s)	1392
MroNI	GCCGGC	1 cut(s)	752
MscI	TGGCCA	1 cut(s)	857
MslI	CAYNNNNRTG	2 cut(s)	1044, 1560
Msp20I	TGGCCA	1 cut(s)	857
MspA1I	CMGCKG	2 cut(s)	321, 1217
MspCI	CTTAAG	2 cut(s)	1723, 2234
MspI	CCGG	4 cut(s)	24, 35, 43, 753
MspR9I	CCNGG	5 cut(s)	44, 146, 167, 2575, 2814
MunI	CAATTG	1 cut(s)	841
Mva1269I	GAATGC	2 cut(s)	1879, 2167
MvaI	CCWGG	4 cut(s)	146, 167, 2575, 2814
MvnI	CGCG	3 cut(s)	396, 2921, 2923
MwoI	GCNNNNNNNGC	6 cut(s)	155, 393, 846, 1421, 2294, 2579
NaeI	GCCGGC	1 cut(s)	754
NciI	CCSGG	1 cut(s)	44
NcoI	CCATGG	1 cut(s)	2000
NdeI	CATATG	1 cut(s)	2673
NdeII	GATC	9 cut(s)	256, 298, 671, 865, 1313, 1958, 2653, 2739, 2970
NgoMIV	GCCGGC	1 cut(s)	752
NlaIV	GGNNCC	6 cut(s)	47, 1564, 1852, 1960, 2010, 2224
NmuCI	GTSAC	3 cut(s)	103, 734, 1106
NruI	TCGCGA	1 cut(s)	396
NsiI	ATGCAT	1 cut(s)	1392
NspI	RCATGY	5 cut(s)	563, 940, 1390, 1419, 2582
NspV	TTCGAA	2 cut(s)	1361, 1507
OliI	CACNNNNGTG	1 cut(s)	1560
PaeI	GCATGC	3 cut(s)	1390, 1419, 2582
PaeR7I	CTCGAG	2 cut(s)	2189, 2442
PagI	TCATGA	1 cut(s)	366
PaqCI	CACCTGC	1 cut(s)	722
PasI	CCCWGGG	1 cut(s)	2813
PctI	GAATGC	2 cut(s)	1879, 2167
PdiI	GCCGGC	1 cut(s)	754
PflMI	CCANNNNNTGG	1 cut(s)	1654
PfoI	TCCNGGA	1 cut(s)	165
PkrI	GCNGC	6 cut(s)	417, 509, 748, 751, 1404, 1542
PleI	GAGTC	5 cut(s)	233, 1084, 1199, 2503, 2913
PpsI	GAGTC	5 cut(s)	233, 1084, 1199, 2503, 2913
PpuMI	RGGWCCY	2 cut(s)	1850, 2039
Psp124BI	GAGCTC	1 cut(s)	366
Psp5II	RGGWCCY	2 cut(s)	1850, 2039
Psp6I	CCWGG	4 cut(s)	144, 165, 2573, 2812
PspFI	CCCAGC	1 cut(s)	1180
PspGI	CCWGG	4 cut(s)	144, 165, 2573, 2812
PspN4I	GGNNCC	6 cut(s)	47, 1564, 1852, 1960, 2010, 2224
PspPI	GGNCC	6 cut(s)	46, 373, 1850, 2039, 2222, 2810
PspPPI	RGGWCCY	2 cut(s)	1850, 2039
PsrI	GAACNNNNNNTAC	2 cut(s)	1571, 1603
PstNI	CAGNNNCTG	1 cut(s)	2695
PsuI	RGATCY	4 cut(s)	865, 1313, 1958, 2970
PvuII	CAGCTG	2 cut(s)	321, 1217
RruI	TCGCGA	1 cut(s)	396
RsaI	GTAC	7 cut(s)	125, 401, 440, 1447, 1526, 1550, 2947
RsaNI	GTAC	7 cut(s)	124, 400, 439, 1446, 1525, 1549, 2946
RseI	CAYNNNNRTG	2 cut(s)	1044, 1560
SacI	GAGCTC	1 cut(s)	366
SatI	GCNGC	6 cut(s)	416, 508, 747, 750, 1403, 1541
Sau3AI	GATC	9 cut(s)	256, 298, 671, 865, 1313, 1958, 2653, 2739, 2970
Sau96I	GGNCC	6 cut(s)	46, 373, 1850, 2039, 2222, 2810
ScaI	AGTACT	1 cut(s)	401
SchI	GAGTC	5 cut(s)	233, 1085, 1199, 2503, 2914
ScrFI	CCNGG	5 cut(s)	44, 146, 167, 2575, 2814
SduI	GDGCHC	4 cut(s)	366, 1567, 2011, 2076
SfaNI	GCATC	5 cut(s)	495, 631, 956, 2275, 2419
SfcI	CTRYAG	2 cut(s)	1859, 2289
Sfr274I	CTCGAG	2 cut(s)	2189, 2442
SfuI	TTCGAA	2 cut(s)	1361, 1507
SinI	GGWCC	3 cut(s)	46, 1850, 2039
SlaI	CTCGAG	2 cut(s)	2189, 2442
SmiMI	CAYNNNNRTG	2 cut(s)	1044, 1560
SphI	GCATGC	3 cut(s)	1390, 1419, 2582
SsiI	CCGC	7 cut(s)	108, 339, 507, 2297, 2345, 2432, 2677
SspI	AATATT	3 cut(s)	979, 1018, 1533
SspMI	CTAG	4 cut(s)	536, 773, 971, 2313
SstI	GAGCTC	1 cut(s)	366
StyD4I	CCNGG	5 cut(s)	42, 144, 165, 2573, 2812
StyI	CCWWGG	5 cut(s)	216, 909, 1572, 2000, 2525
TaaI	ACNGT	7 cut(s)	1627, 1863, 1903, 2426, 2456, 2568, 2950
TaqII	GACCGA	1 cut(s)	2595
TatI	WGTACW	4 cut(s)	399, 438, 1524, 1548
TauI	GCSGC	1 cut(s)	510
TscAI	CASTG	3 cut(s)	483, 748, 2459
TseFI	GTSAC	3 cut(s)	103, 734, 1106
TseI	GCWGC	5 cut(s)	415, 746, 749, 1402, 1540
Tsp45I	GTSAC	3 cut(s)	103, 734, 1106
TspGWI	ACGGA	4 cut(s)	232, 2607, 2842, 2983
TspRI	CASTG	3 cut(s)	483, 748, 2459
Van91I	CCANNNNNTGG	1 cut(s)	1654
Vha464I	CTTAAG	2 cut(s)	1723, 2234
VpaK11BI	GGWCC	3 cut(s)	46, 1850, 2039
XagI	CCTNNNNNAGG	1 cut(s)	2046
XapI	RAATTY	6 cut(s)	651, 884, 1260, 1453, 1787, 2365
XceI	RCATGY	5 cut(s)	563, 940, 1390, 1419, 2582
XcmI	CCANNNNNNNNNTGG	1 cut(s)	16
XhoI	CTCGAG	2 cut(s)	2189, 2442
XspI	CTAG	4 cut(s)	536, 773, 971, 2313
ZrmI	AGTACT	1 cut(s)	401
Zsp2I	ATGCAT	1 cut(s)	1392

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.