Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0010977

Physical Location & Seq

Genomic Coordinates Reverse (-)

3491738 .. 3499094

Gene Structure 7357 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom03g04480.16

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2661 bp

ATGATGGGCCTCTTCAACTGCTATGTTCGCGAATACTCGTTTGTAAAGCAGCAACAGACAGCTATAAAAATGTACAAGCTTGTTGGGGAGGAGAGGTTTTTGCTATGGGCAGTGTGTAGCATCCAGTTACAGGTATTTTGTGGCAATGGGAGTGAGAAGCTATTAGCTTTAGCCGAAGGTTTGATTAAGAAACACGTTGCCTCCCACAGCTTGCATGAGCCTGAAGCTCTTATGGTCTATGTTTCCATATTGGAACAACAAGCCAAATATGGGGATGCTCTGGAAATTCTCTCTGGAAAGTTAGGATCGCTTTTGATGATTGAAGTTGATAAGCTACGCATACAGGGAAGGCTTCATGCTCGGGCAGGTGACTACACTGCTGCTGCCAGCATATTTCAAAAAATCCTAGAATTATGTCCTGATGATTGGGATTGTTTCCTACATTACCTCGGTTGTTTGCTGGAGGATGACAGCAATTGGTGCAATATGGCCAACATAGATCCATTTCATCCTCCAAAATTCATTGAGTGCAAGATTTTAAACTTGGGAGATGAAGTGTTTGATTCTCGCATGTCAAATGCATCGGATTTTGTACAGAAGTTACTAGAAAACATCGGTGATAATTTTCTAAGGTCTCCATACTTGGCAAATATTGAAATTGAAAGGAGGAGGCACTTACATGGCAAAGGGGATGCTGAGAAGTTAATGGAGTCTCTAGTCCAGTACTTTCTAAGGTGTGGTCACTTGGCCTGCTTCACTTATGATGTCGAGATGTTTCTTGAAGTTTTAACGCCTGATAAGAAGGCACAATTGCTGGGGAAGTTAAAGGAAAGCTCTCACTCCATTTCAGCTGTTCCAACAAAAGTTCTTGGGCAGTCAATAACACTCTTTAAATTTCAAGAATTGATAGGGAACACATTCAAACTTCCTGTGGGTGCCCTTAAGTCTGCTTTGCAGATGGTGGAGATGTATTGTCAAAACCTTCCACTTTCGAAGGACTTGGATTCACAAGAGAGTATGCATGGTGAGGAGCTGCTCTCAATGGCATGCAATGCGTTGATTCAGTTATTTTGGCGTACAAGGAATTTCGGCTACTATTTTGAAGCAATTATGGTATTGGAATTCGGCTTGACCATTCGGAAATATGTATGGGAGTACAAGATATTGTTGCTGCACTTATATTCCCACTTAGGTGCCCTTTCGTTAGCTTATGAGTGGTTCCGATCATTGGATGTGAAAAATATCTTGATGGAAACTGTTTCACACCATATTTTACCCCAGATGTTGGTGTCTCCCCTGTGGGCAGATCTAAATAATCTGCTAAAGGATTATCTCAAGTTTATGGATGACCACTTAAGAGAATCAGCAGACCTTACATTTCTTGCCTATCGTCATAGAAATTACTCAAAAGTAATTGAATTTATCCAGTTTAAAGAAAGACTACAACAATCCAATCAGTATCTAGTGGCGAAGGTTGAAGGACCCATTCTACAGTTAAAGCAGAATGCAGATACAATTGATGATGAAGAGACTGTTCTTGAGAGCTTGAAATGCGGTGTTCACTTTGTTGAACTCTCTAATGAGATTGGATCCAAATCGTTGACCTTCAATGAAGATTTGCAGTCCCGTCCATGGTGGACACCAACTGCAGAAAGAAACTATCTTTTAGGTCCTTTTGAAGGAGTATCCTATTGTCCAAAAGAGAACTCGGTGAAAGAAAGGGAAGCAAATGTAAGGAGAGTTATCGAGAGGAAGTCTCTTCTCCCTCGGATGATTTATTTGTCCATACAGAATGCTTCAACTTCACTCAAAGAAAATCTCGAGGCCAATGGAACGACATCTGGCTCTAAAGTCCCCTCAGAACTTAAGGGTTTGCTTGAGCGCTATGCAAAAATGTTGGGATTCACACTAAATGATGCTATAGAAGCGGTTTTGGGGGTCTCTAGTGGCCTAAAGTCATTTGAGGTCTTTGGTGCGGACTTGATTGACTGGATAAATTTTTCTGTGTTTCTGAATGCATGGAACTTGAGTTCCCATGAAATTGGGCAAGCAAATGGTGATGCAGGTCTGTCTCTAGCATGGCACTGTGTGGATTCGCTGTTGGAGAAGTATGTTTCGGAGAAAGTTAGCTCGATGGAGACTCTAATTAGCTCTCCCTGGGTTGATGTTCCACTTCTGGTTCAGTTGGTTACAGAACCTTTGGCCTGGCACGCTCTTGTAATTCAATCTTGTACTCGGTCATCTCATCCGTCTGGAAAGAAGAAGAAGAAAACTGGAGTTGTGGATCATTCTAGTCTGTCCCATATGCGGGATTCTGTCCAGTCTCTGTGTAATACTCTGGAAAAAGTTATGAAATGGTTAAGAGAACAGATCAACAGGCCAGAGGATGAAAGTTTAGACACTTTACTCTCCTCTTTGCAGAATAAAGGACAGAATGAAGGCCCTGGGCAGGTTTTCCATATCCTAGAAACTTATATCTCATCCGTAGATGACACAGAGGTCGGTGATAGAATTTCCCGAGCATTGAAGTCTTGGAGTCCTCCTGATGTTGCACGGAAGCTAATAACTGGGAAATGTACGGTGCTGTCTGAGTTTCTAAGGATCTGTGGATCCAAACTAAAGGTGTTGCAGACATTGAAACAGCAGATAGCTCAAGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005829 GO:0005911 GO:0006464 GO:0006473 GO:0006474 GO:0006807 GO:0006810 GO:0006897 GO:0006909 GO:0006911 GO:0006955 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0010324 GO:0010467 GO:0016043 GO:0016192 GO:0017196 GO:0018193 GO:0018206 GO:0019538 GO:0030054 GO:0031248 GO:0031365 GO:0031414 GO:0031416 GO:0032991 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043543 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0051604 GO:0055044 GO:0061024 GO:0071704 GO:0071840 GO:0098657 GO:0099024 GO:1901564 GO:1902493 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NatB_MDM20

Protein Analysis

887

Amino Acids

100.62

Weight (kDa)

5.97

Isoelectric Point (pI)

44.09

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010977)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58450 AT5G58450
fragaria_vesca	FvH4_3g39760
malus_domestica	MD03G1056500.v1.1 MD11G1058300.v1.1
prunus_persica	Prupe.6G048400_v2.0.a1
pyrus_communis	pycom03g04480 pycom11g04830
rosa_chinensis	RchiOBHm_Chr5g0071641
rosa_laevigata	RLG00000036235
rosa_multiflora	Rmu_sc0005745.1_g000032
rosa_roxburghii	Rroxscaffold_1G00009550
rosa_rugosa	Rorug05G0413800 Rorug05G0413800 Rorug05G0413800
rosa_samantha	Rh5AG469800 Rh5BG489100 Rh5CG513100 Rh5DG499700
rosa_wichuraiana	Rw5G043650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	356
AasI	GACNNNNNNGTC	1 cut(s)	2498
Acc36I	ACCTGC	3 cut(s)	356, 2054, 2440
AccB1I	GGYRCC	2 cut(s)	935, 1193
AccB7I	CCANNNNNTGG	1 cut(s)	1285
AccII	CGCG	1 cut(s)	30
AciI	CCGC	4 cut(s)	1554, 1928, 1976, 2308
AclWI	GGATC	8 cut(s)	313, 494, 1584, 1597, 2292, 2604, 2609, 2617
AcoI	YGGCCR	1 cut(s)	489
AcsI	RAATTY	8 cut(s)	285, 518, 893, 1084, 1121, 1418, 1996, 2511
AcuI	CTGAAG	1 cut(s)	243
AdeI	CACNNNGTG	1 cut(s)	2089
AfaI	GTAC	7 cut(s)	74, 594, 725, 1078, 1157, 2233, 2578
AfeI	AGCGCT	1 cut(s)	1883
AfiI	CCNNNNNNNGG	9 cut(s)	130, 270, 1228, 1285, 1632, 1679, 2307, 2308, 2449
AflII	CTTAAG	3 cut(s)	941, 1354, 1865
AflIII	ACRYGT	1 cut(s)	193
AjnI	CCWGG	3 cut(s)	2156, 2204, 2443
AleI	CACNNNNGTG	1 cut(s)	1191
AloI	GAACNNNNNNTCC	4 cut(s)	2014, 2015, 2046, 2047
Alw26I	GTCTC	9 cut(s)	639, 717, 1296, 1523, 1761, 1945, 2076, 2132, 2328
AlwI	GGATC	8 cut(s)	313, 494, 1584, 1597, 2292, 2604, 2609, 2617
AlwNI	CAGNNNCTG	1 cut(s)	2326
Ama87I	CYCGRG	3 cut(s)	360, 1820, 2517
Aor51HI	AGCGCT	1 cut(s)	1883
AoxI	GGCC	8 cut(s)	7, 489, 747, 1824, 1948, 2202, 2378, 2440
ApeKI	GCWGC	5 cut(s)	49, 380, 383, 1033, 1171
ApoI	RAATTY	8 cut(s)	285, 518, 893, 1084, 1121, 1418, 1996, 2511
Asp700I	GAANNNNTTC	1 cut(s)	917
AspLEI	GCGC	1 cut(s)	1884
AspS9I	GGNCC	4 cut(s)	7, 1481, 1670, 2441
AsuHPI	GGTGA	6 cut(s)	380, 629, 1037, 1723, 2069, 2516
AsuII	TTCGAA	1 cut(s)	992
AvaI	CYCGRG	3 cut(s)	360, 1820, 2517
AvaII	GGWCC	2 cut(s)	1481, 1670
BaeGI	GKGCMC	2 cut(s)	940, 1198
BalI	TGGCCA	1 cut(s)	491
BamHI	GGATCC	2 cut(s)	1589, 2609
BanI	GGYRCC	2 cut(s)	935, 1193
BbvI	GCAGC	5 cut(s)	61, 367, 370, 1020, 1158
BccI	CCATC	3 cut(s)	952, 1243, 2128
BciT130I	CCWGG	3 cut(s)	2158, 2206, 2445
BciVI	GTATCC	1 cut(s)	1696
BcoDI	GTCTC	9 cut(s)	639, 717, 1296, 1523, 1761, 1945, 2076, 2132, 2328
BfaI	CTAG	8 cut(s)	407, 605, 716, 1463, 1944, 2075, 2292, 2465
BfmI	CTRYAG	3 cut(s)	1490, 1647, 1920
BfoI	RGCGCY	1 cut(s)	1885
BfrI	CTTAAG	3 cut(s)	941, 1354, 1865
BfuAI	ACCTGC	3 cut(s)	356, 2054, 2440
BfuI	GTATCC	1 cut(s)	1696
BglII	AGATCT	1 cut(s)	1306
BisI	GCNGC	5 cut(s)	50, 381, 384, 1034, 1172
BlsI	GCNGC	5 cut(s)	51, 382, 385, 1035, 1173
BmcAI	AGTACT	1 cut(s)	725
Bme1390I	CCNGG	3 cut(s)	2158, 2206, 2445
Bme18I	GGWCC	2 cut(s)	1481, 1670
BmeT110I	CYCGRG	3 cut(s)	360, 1820, 2517
BmgT120I	GGNCC	4 cut(s)	7, 1481, 1670, 2441
BmiI	GGNNCC	6 cut(s)	937, 1195, 1220, 1483, 1591, 2611
BmrFI	CCNGG	3 cut(s)	2158, 2206, 2445
BmrI	ACTGGG	1 cut(s)	2577
BmsI	GCATC	6 cut(s)	129, 265, 590, 682, 1906, 2050
BmuI	ACTGGG	1 cut(s)	2577
BplI	GAGNNNNNCTC	4 cut(s)	1022, 1054, 1741, 1773
BpmI	CTGGAG	2 cut(s)	482, 2295
Bpu14I	TTCGAA	1 cut(s)	992
BpuEI	CTTGAG	5 cut(s)	1319, 1559, 1898, 2047, 2637
BsaBI	GATNNNNATC	1 cut(s)	1594
BsaI	GGTCTC	2 cut(s)	639, 1945
BsaJI	CCNNGG	7 cut(s)	448, 1631, 1766, 2156, 2157, 2443, 2444
BsaXI	ACNNNNNCTCC	6 cut(s)	80, 110, 185, 215, 540, 570
Bsc4I	CCNNNNNNNGG	9 cut(s)	130, 270, 1228, 1285, 1632, 1679, 2307, 2308, 2449
Bse1I	ACTGG	7 cut(s)	124, 721, 1426, 1994, 2278, 2320, 2572
Bse3DI	GCAATG	2 cut(s)	151, 1057
Bse8I	GATNNNNATC	1 cut(s)	1594
BseBI	CCWGG	3 cut(s)	2158, 2206, 2445
BseDI	CCNNGG	7 cut(s)	448, 1631, 1766, 2156, 2157, 2443, 2444
BseJI	GATNNNNATC	1 cut(s)	1594
BseLI	CCNNNNNNNGG	9 cut(s)	130, 270, 1228, 1285, 1632, 1679, 2307, 2308, 2449
BseMI	GCAATG	2 cut(s)	151, 1057
BseMII	CTCAG	3 cut(s)	687, 1872, 2580
BseNI	ACTGG	7 cut(s)	124, 721, 1426, 1994, 2278, 2320, 2572
BseRI	GAGGAG	4 cut(s)	104, 682, 1043, 2401
BseSI	GKGCMC	2 cut(s)	940, 1198
BseXI	GCAGC	5 cut(s)	61, 367, 370, 1020, 1158
BseYI	CCCAGC	1 cut(s)	814
BsgI	GTGCAG	1 cut(s)	1157
Bsh1236I	CGCG	1 cut(s)	30
BshFI	GGCC	8 cut(s)	9, 491, 749, 1826, 1950, 2204, 2380, 2442
BshNI	GGYRCC	2 cut(s)	935, 1193
BsiHKCI	CYCGRG	3 cut(s)	360, 1820, 2517
BslFI	GGGAC	3 cut(s)	1609, 1838, 2284
BslI	CCNNNNNNNGG	9 cut(s)	130, 270, 1228, 1285, 1632, 1679, 2307, 2308, 2449
BsmAI	GTCTC	9 cut(s)	639, 717, 1296, 1523, 1761, 1945, 2076, 2132, 2328
BsmFI	GGGAC	3 cut(s)	1609, 1838, 2284
BsmI	GAATGC	3 cut(s)	1510, 1798, 2020
BsnI	GGCC	8 cut(s)	9, 491, 749, 1826, 1950, 2204, 2380, 2442
Bso31I	GGTCTC	2 cut(s)	639, 1945
BsoBI	CYCGRG	3 cut(s)	360, 1820, 2517
Bsp119I	TTCGAA	1 cut(s)	992
Bsp1286I	GDGCHC	2 cut(s)	940, 1198
Bsp1407I	TGTACA	2 cut(s)	72, 592
Bsp143I	GATC	9 cut(s)	305, 499, 1223, 1306, 1589, 2284, 2370, 2601, 2609
Bsp19I	CCATGG	1 cut(s)	1631
Bsp68I	TCGCGA	1 cut(s)	30
BspACI	CCGC	4 cut(s)	1554, 1928, 1976, 2308
BspANI	GGCC	8 cut(s)	9, 491, 749, 1826, 1950, 2204, 2380, 2442
BspCNI	CTCAG	3 cut(s)	688, 1871, 2581
BspFNI	CGCG	1 cut(s)	30
BspLI	GGNNCC	6 cut(s)	937, 1195, 1220, 1483, 1591, 2611
BspMAI	CTGCAG	1 cut(s)	1651
BspMI	ACCTGC	3 cut(s)	356, 2054, 2440
BspPI	GGATC	8 cut(s)	313, 494, 1584, 1597, 2292, 2604, 2609, 2617
BspT104I	TTCGAA	1 cut(s)	992
BspT107I	GGYRCC	2 cut(s)	935, 1193
BspTI	CTTAAG	3 cut(s)	941, 1354, 1865
BspTNI	GGTCTC	2 cut(s)	639, 1945
BsrDI	GCAATG	2 cut(s)	151, 1057
BsrGI	TGTACA	2 cut(s)	72, 592
BsrI	ACTGG	7 cut(s)	124, 721, 1426, 1994, 2278, 2320, 2572
BssECI	CCNNGG	7 cut(s)	448, 1631, 1766, 2156, 2157, 2443, 2444
BssMI	GATC	9 cut(s)	305, 499, 1223, 1306, 1589, 2284, 2370, 2601, 2609
BssT1I	CCWWGG	1 cut(s)	1631
Bst2UI	CCWGG	3 cut(s)	2158, 2206, 2445
Bst4CI	ACNGT	5 cut(s)	1258, 1494, 1534, 2087, 2581
Bst6I	CTCTTC	3 cut(s)	17, 1521, 1764
BstAFI	CTTAAG	3 cut(s)	941, 1354, 1865
BstAPI	GCANNNNNTGC	2 cut(s)	480, 1052
BstAUI	TGTACA	2 cut(s)	72, 592
BstBI	TTCGAA	1 cut(s)	992
BstC8I	GCNNGC	6 cut(s)	212, 388, 751, 1048, 2049, 2211
BstDEI	CTNAG	7 cut(s)	629, 696, 731, 1189, 1858, 2589, 2597
BstDSI	CCRYGG	1 cut(s)	1631
BstENI	CCTNNNNNAGG	1 cut(s)	1677
BstFNI	CGCG	1 cut(s)	30
BstH2I	RGCGCY	1 cut(s)	1885
BstHHI	GCGC	1 cut(s)	1884
BstKTI	GATC	9 cut(s)	308, 502, 1226, 1309, 1592, 2287, 2373, 2604, 2612
BstMAI	GTCTC	9 cut(s)	639, 717, 1296, 1523, 1761, 1945, 2076, 2132, 2328
BstMBI	GATC	9 cut(s)	305, 499, 1223, 1306, 1589, 2284, 2370, 2601, 2609
BstMWI	GCNNNNNNNGC	6 cut(s)	27, 480, 1052, 1551, 1925, 2210
BstNI	CCWGG	3 cut(s)	2158, 2206, 2445
BstNSI	RCATGY	2 cut(s)	574, 1050
BstSCI	CCNGG	3 cut(s)	2156, 2204, 2443
BstSFI	CTRYAG	3 cut(s)	1490, 1647, 1920
BstSLI	GKGCMC	2 cut(s)	940, 1198
BstUI	CGCG	1 cut(s)	30
BstV1I	GCAGC	5 cut(s)	61, 367, 370, 1020, 1158
BstX2I	RGATCY	5 cut(s)	499, 1306, 1589, 2601, 2609
BstXI	CCANNNNNNTGG	1 cut(s)	2042
BstYI	RGATCY	5 cut(s)	499, 1306, 1589, 2601, 2609
BsuI	GTATCC	1 cut(s)	1696
BsuRI	GGCC	8 cut(s)	9, 491, 749, 1826, 1950, 2204, 2380, 2442
BtgI	CCRYGG	1 cut(s)	1631
BtsI	GCAGTG	2 cut(s)	117, 375
BtsIMutI	CAGTG	3 cut(s)	117, 375, 2083
BtuMI	TCGCGA	1 cut(s)	30
BveI	ACCTGC	3 cut(s)	356, 2054, 2440
Cac8I	GCNNGC	6 cut(s)	212, 388, 751, 1048, 2049, 2211
CaiI	CAGNNNCTG	1 cut(s)	2326
CfoI	GCGC	1 cut(s)	1884
Cfr13I	GGNCC	4 cut(s)	7, 1481, 1670, 2441
Csp6I	GTAC	7 cut(s)	73, 593, 724, 1077, 1156, 2232, 2577
CviQI	GTAC	7 cut(s)	73, 593, 724, 1077, 1156, 2232, 2577
DdeI	CTNAG	7 cut(s)	629, 696, 731, 1189, 1858, 2589, 2597
DpnI	GATC	9 cut(s)	307, 501, 1225, 1308, 1591, 2286, 2372, 2603, 2611
DpnII	GATC	9 cut(s)	305, 499, 1223, 1306, 1589, 2284, 2370, 2601, 2609
DraI	TTTAAA	3 cut(s)	540, 892, 1432
DraIII	CACNNNGTG	1 cut(s)	2089
DrdI	GACNNNNNNGTC	1 cut(s)	2498
DseDI	GACNNNNNNGTC	1 cut(s)	2498
EaeI	YGGCCR	1 cut(s)	489
Eam1104I	CTCTTC	3 cut(s)	17, 1521, 1764
EarI	CTCTTC	3 cut(s)	17, 1521, 1764
Eco130I	CCWWGG	1 cut(s)	1631
Eco31I	GGTCTC	2 cut(s)	639, 1945
Eco47I	GGWCC	2 cut(s)	1481, 1670
Eco47III	AGCGCT	1 cut(s)	1883
Eco57I	CTGAAG	1 cut(s)	243
Eco88I	CYCGRG	3 cut(s)	360, 1820, 2517
EcoNI	CCTNNNNNAGG	1 cut(s)	1677
EcoO109I	RGGNCCY	3 cut(s)	1481, 1670, 2441
EcoRI	GAATTC	1 cut(s)	1121
EcoRII	CCWGG	3 cut(s)	2156, 2204, 2443
EcoT14I	CCWWGG	1 cut(s)	1631
EcoT22I	ATGCAT	3 cut(s)	583, 1023, 2020
ErhI	CCWWGG	1 cut(s)	1631
FaqI	GGGAC	3 cut(s)	1609, 1838, 2284
FauI	CCCGC	1 cut(s)	2301
FauNDI	CATATG	1 cut(s)	2304
Fnu4HI	GCNGC	5 cut(s)	50, 381, 384, 1034, 1172
Fsp4HI	GCNGC	5 cut(s)	50, 381, 384, 1034, 1172
FspBI	CTAG	8 cut(s)	407, 605, 716, 1463, 1944, 2075, 2292, 2465
GlaI	GCGC	1 cut(s)	1883
GluI	GCNGC	5 cut(s)	50, 381, 384, 1034, 1172
GsaI	CCCAGC	1 cut(s)	818
GsuI	CTGGAG	2 cut(s)	482, 2295
HaeII	RGCGCY	1 cut(s)	1885
HaeIII	GGCC	8 cut(s)	9, 491, 749, 1826, 1950, 2204, 2380, 2442
HhaI	GCGC	1 cut(s)	1884
Hin6I	GCGC	1 cut(s)	1882
HinP1I	GCGC	1 cut(s)	1882
HincII	GTYRAC	1 cut(s)	1602
HindII	GTYRAC	1 cut(s)	1602
HindIII	AAGCTT	1 cut(s)	77
HphI	GGTGA	6 cut(s)	380, 629, 1037, 1723, 2069, 2516
Hpy166II	GTNNAC	3 cut(s)	1561, 1602, 1638
Hpy188I	TCNGA	9 cut(s)	586, 1140, 1223, 1770, 1861, 2013, 2119, 2590, 2660
Hpy8I	GTNNAC	3 cut(s)	1561, 1602, 1638
HpyCH4III	ACNGT	5 cut(s)	1258, 1494, 1534, 2087, 2581
HpyCH4IV	ACGT	1 cut(s)	195
HpyF10VI	GCNNNNNNNGC	6 cut(s)	27, 480, 1052, 1551, 1925, 2210
HpyF3I	CTNAG	7 cut(s)	629, 696, 731, 1189, 1858, 2589, 2597
HpySE526I	ACGT	1 cut(s)	195
HspAI	GCGC	1 cut(s)	1882
Kzo9I	GATC	9 cut(s)	305, 499, 1223, 1306, 1589, 2284, 2370, 2601, 2609
LmnI	GCTCC	1 cut(s)	1030
Lsp1109I	GCAGC	5 cut(s)	61, 367, 370, 1020, 1158
LweI	GCATC	6 cut(s)	129, 265, 590, 682, 1906, 2050
MaeI	CTAG	8 cut(s)	407, 605, 716, 1463, 1944, 2075, 2292, 2465
MaeII	ACGT	1 cut(s)	195
MaeIII	GTNAC	5 cut(s)	126, 368, 600, 740, 2188
MalI	GATC	9 cut(s)	307, 501, 1225, 1308, 1591, 2286, 2372, 2603, 2611
MboI	GATC	9 cut(s)	305, 499, 1223, 1306, 1589, 2284, 2370, 2601, 2609
MboII	GAAGA	7 cut(s)	4, 1538, 1625, 1751, 2272, 2275, 2278
MfeI	CAATTG	3 cut(s)	475, 809, 1515
MflI	RGATCY	5 cut(s)	499, 1306, 1589, 2601, 2609
MhlI	GDGCHC	2 cut(s)	940, 1198
MlsI	TGGCCA	1 cut(s)	491
MluNI	TGGCCA	1 cut(s)	491
MlyI	GAGTC	3 cut(s)	719, 2134, 2545
MmeI	TCCRAC	2 cut(s)	881, 2082
Mox20I	TGGCCA	1 cut(s)	491
Mph1103I	ATGCAT	3 cut(s)	583, 1023, 2020
MroXI	GAANNNNTTC	1 cut(s)	917
MscI	TGGCCA	1 cut(s)	491
MslI	CAYNNNNRTG	2 cut(s)	678, 1191
Msp20I	TGGCCA	1 cut(s)	491
MspA1I	CMGCKG	1 cut(s)	851
MspCI	CTTAAG	3 cut(s)	941, 1354, 1865
MspR9I	CCNGG	3 cut(s)	2158, 2206, 2445
MunI	CAATTG	3 cut(s)	475, 809, 1515
Mva1269I	GAATGC	3 cut(s)	1510, 1798, 2020
MvaI	CCWGG	3 cut(s)	2158, 2206, 2445
MvnI	CGCG	1 cut(s)	30
MwoI	GCNNNNNNNGC	6 cut(s)	27, 480, 1052, 1551, 1925, 2210
NcoI	CCATGG	1 cut(s)	1631
NdeI	CATATG	1 cut(s)	2304
NdeII	GATC	9 cut(s)	305, 499, 1223, 1306, 1589, 2284, 2370, 2601, 2609
NlaIV	GGNNCC	6 cut(s)	937, 1195, 1220, 1483, 1591, 2611
NmuCI	GTSAC	2 cut(s)	368, 740
NruI	TCGCGA	1 cut(s)	30
NsiI	ATGCAT	3 cut(s)	583, 1023, 2020
NspI	RCATGY	2 cut(s)	574, 1050
NspV	TTCGAA	1 cut(s)	992
OliI	CACNNNNGTG	1 cut(s)	1191
PaeI	GCATGC	1 cut(s)	1050
PaeR7I	CTCGAG	1 cut(s)	1820
PaqCI	CACCTGC	1 cut(s)	356
PasI	CCCWGGG	2 cut(s)	2157, 2444
PctI	GAATGC	3 cut(s)	1510, 1798, 2020
PdmI	GAANNNNTTC	1 cut(s)	917
PfeI	GAWTC	7 cut(s)	563, 1004, 1060, 1361, 1902, 2093, 2312
PflMI	CCANNNNNTGG	1 cut(s)	1285
PkrI	GCNGC	5 cut(s)	51, 382, 385, 1035, 1173
PleI	GAGTC	3 cut(s)	718, 2134, 2544
PpsI	GAGTC	3 cut(s)	718, 2134, 2544
PpuMI	RGGWCCY	2 cut(s)	1481, 1670
Psp5II	RGGWCCY	2 cut(s)	1481, 1670
Psp6I	CCWGG	3 cut(s)	2156, 2204, 2443
PspFI	CCCAGC	1 cut(s)	814
PspGI	CCWGG	3 cut(s)	2156, 2204, 2443
PspN4I	GGNNCC	6 cut(s)	937, 1195, 1220, 1483, 1591, 2611
PspPI	GGNCC	4 cut(s)	7, 1481, 1670, 2441
PspPPI	RGGWCCY	2 cut(s)	1481, 1670
PstI	CTGCAG	1 cut(s)	1651
PstNI	CAGNNNCTG	1 cut(s)	2326
PsuI	RGATCY	5 cut(s)	499, 1306, 1589, 2601, 2609
PvuII	CAGCTG	1 cut(s)	851
RruI	TCGCGA	1 cut(s)	30
RsaI	GTAC	7 cut(s)	74, 594, 725, 1078, 1157, 2233, 2578
RsaNI	GTAC	7 cut(s)	73, 593, 724, 1077, 1156, 2232, 2577
RseI	CAYNNNNRTG	2 cut(s)	678, 1191
SatI	GCNGC	5 cut(s)	50, 381, 384, 1034, 1172
Sau3AI	GATC	9 cut(s)	305, 499, 1223, 1306, 1589, 2284, 2370, 2601, 2609
Sau96I	GGNCC	4 cut(s)	7, 1481, 1670, 2441
ScaI	AGTACT	1 cut(s)	725
SchI	GAGTC	3 cut(s)	719, 2134, 2545
ScrFI	CCNGG	3 cut(s)	2158, 2206, 2445
SduI	GDGCHC	2 cut(s)	940, 1198
SfaNI	GCATC	6 cut(s)	129, 265, 590, 682, 1906, 2050
SfcI	CTRYAG	3 cut(s)	1490, 1647, 1920
Sfr274I	CTCGAG	1 cut(s)	1820
SfuI	TTCGAA	1 cut(s)	992
SinI	GGWCC	2 cut(s)	1481, 1670
SlaI	CTCGAG	1 cut(s)	1820
SmiMI	CAYNNNNRTG	2 cut(s)	678, 1191
SmlI	CTYRAG	9 cut(s)	941, 1334, 1354, 1538, 1820, 1865, 1877, 2026, 2652
SmoI	CTYRAG	9 cut(s)	941, 1334, 1354, 1538, 1820, 1865, 1877, 2026, 2652
SphI	GCATGC	1 cut(s)	1050
SsiI	CCGC	4 cut(s)	1554, 1928, 1976, 2308
SspI	AATATT	1 cut(s)	652
SspMI	CTAG	8 cut(s)	407, 605, 716, 1463, 1944, 2075, 2292, 2465
StyD4I	CCNGG	3 cut(s)	2156, 2204, 2443
StyI	CCWWGG	1 cut(s)	1631
TaaI	ACNGT	5 cut(s)	1258, 1494, 1534, 2087, 2581
TaiI	ACGT	1 cut(s)	198
TaqI	TCGA	5 cut(s)	768, 992, 1746, 1821, 2132
TaqII	GACCGA	1 cut(s)	2226
TatI	WGTACW	5 cut(s)	72, 592, 723, 1155, 2231
TfiI	GAWTC	7 cut(s)	563, 1004, 1060, 1361, 1902, 2093, 2312
TscAI	CASTG	3 cut(s)	117, 382, 2090
TseFI	GTSAC	2 cut(s)	368, 740
TseI	GCWGC	5 cut(s)	49, 380, 383, 1033, 1171
Tsp45I	GTSAC	2 cut(s)	368, 740
TspGWI	ACGGA	3 cut(s)	2238, 2473, 2569
TspRI	CASTG	3 cut(s)	117, 382, 2090
Van91I	CCANNNNNTGG	1 cut(s)	1285
Vha464I	CTTAAG	3 cut(s)	941, 1354, 1865
VpaK11BI	GGWCC	2 cut(s)	1481, 1670
XagI	CCTNNNNNAGG	1 cut(s)	1677
XapI	RAATTY	8 cut(s)	285, 518, 893, 1084, 1121, 1418, 1996, 2511
XceI	RCATGY	2 cut(s)	574, 1050
XhoI	CTCGAG	1 cut(s)	1820
XmnI	GAANNNNTTC	1 cut(s)	917
XspI	CTAG	8 cut(s)	407, 605, 716, 1463, 1944, 2075, 2292, 2465
ZrmI	AGTACT	1 cut(s)	725
Zsp2I	ATGCAT	3 cut(s)	583, 1023, 2020

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.