Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0006104

Physical Location & Seq

Genomic Coordinates Forward (+)

16017444 .. 16033881

Gene Structure 16438 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1161800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1473 bp

ATGGAGTTGGAAAGTATCCTTCGTTTCATTGAGAACAAGAACATTTTAGTCACTGGTGCCACTGGCTTTCTAGCAAAGATTTTTGTAGAGAAGATACTGAGGGTGCAACCAAATGTTAAGAAGCTTTACCTTCTTCTTAGAGCTCCTGATACAAAATCAGCTACTCAGCGTTTGAATAATGAGATAATTGGGAAGGATCTTTTTAGAGTGTTGAAGGAAAAATGGGAAGCAGGTATGAACTCAATCATCTCAGAAAAGCTGACTGTGGTGCCTGGAGACATTTCTCGTGAGGATTTAGGCTTGAATGATTCTAGCTTGAGGGAAGAGATGTTGAGTCATATAGATGTCATTGTGAACTTAGCTGCAACTACCAACTTTGATGAAAGATATGATATTGCACTTGCTCTCAATACTTTGGGAGCAATGCATGTATTGAACTTTGCCAAGCAATGTATTCAATTACAGGTGCTGGTCCACGTGTCCACAGCTTATGTTTGCGGCGAAAAGGATGGGCTTTTATTAGAAAACCCATACCGCATGGGCGAGACACTCAATGGGACCACAGGGCTAGACATCGACAATGAAATCAGGCTGCTCCAAGAAAAACTGAAAGAGCTCCGCGCGGAGGAGGCATCAGAACAAGCAATCACTCTGGCCCTCAAAGATTTTGGACTCCAAAGAGCAATTGTATATGGATGGCCAAACACCTACGTGTTTACCAAGGCAATGGGAGAGATGCTAATAGGAGACCTAAAAGAAAATCTACCTGTGGTCATTATTCGCCCTACCATCATCTCCAGCACTTACAAAGAACCTTTTCCTGGTTGGGTTGAAGGAATCAGAACCATTGATAGCATTGCAGTTGGTTATGGTAAAGGAAAATTAACATTCTTTCTCTGCGATATTGATGCAATTGTTGATGTGATACCAGCTGACATGGTTGTGAATGCAATACTTGCTGCAATGGCAGCCCATGCAAATCAACCTGGTGAAGTAATTTATCAAGTAGGATCGTCTATGAGAAATCCTTTAAGATATAGTAATCTTCACGATTATGGATTCCGTTTCTTCACCAAGAAACCATGGATTAATAAAGATGGGACGCCAGTCAAGGTTGGCAAGGTTAAAGTTATGGGCAGCATGGCTAGCTTTCACAGATACATGACTGTCCGTTACTTACTGTTCCTGAAGGGATTGGAGGTGGTTAATACAGCATTTTGCCAGTACTTCAAGGGCACATACGTGGATCTCAGTAGAAAAATCAAATTTGTGATGAGGCTGGTGGAGCTTTACAAACCATACTTGTTCTTCAAAGGAGTCTTCGATGATATGAACACAGAGAAGCTGCAAATGGCAGTGAGAGAAAACACCACAGAGGCAGACATATTTTACTTTGATCCGAAGTGCATAAATTGGGACGACTACATAATGAATGTCCATCTTCCTGGGGTTGTGAAATACGTCTTCAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006139 GO:0006163 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009150 GO:0009259 GO:0009698 GO:0009699 GO:0009987 GO:0010025 GO:0010166 GO:0010345 GO:0012505 GO:0016491 GO:0016620 GO:0016903 GO:0019438 GO:0019637 GO:0019693 GO:0019748 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035336 GO:0035337 GO:0035383 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0046483 GO:0051186 GO:0055086 GO:0055114 GO:0071704 GO:0072521 GO:0080019 GO:1901135 GO:1901360 GO:1901362 GO:1901564 GO:1901568 GO:1901570 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00073 ko04146 ko04212 map00073 map04146 map04212

Pfam Domains

Protein Families

NAD_binding_4 Sterile

Protein Analysis

491

Amino Acids

55.62

Weight (kDa)

7.05

Isoelectric Point (pI)

23.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	15 - 171	3.5e-07	NAD dependent epimerase/dehydratase family
NAD_binding_4	PF07993	17 - 318	8e-76	Male sterility protein
Sterile	PF03015	393 - 490	5e-18	Male sterility protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006104)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44540 AT3G44540 AT3G44540 AT4G33790 AT4G33790 AT5G22500 AT5G22500
fragaria_vesca	FvH4_3g24571 FvH4_3g24571 FvH4_3g24571 FvH4_3g24571 FvH4_3g24571
malus_domestica	MD03G1141800.v1.1 MD11G1161800.v1.1
prunus_persica	Prupe.6G126900_v2.0.a1
pyrus_communis	pycom03g09660 pycom11g13340
rosa_chinensis	RchiOBHm_Chr5g0044101
rosa_laevigata	RLG00000034241
rosa_multiflora	Rmu_sc0000939.1_g000010
rosa_roxburghii	Rroxscaffold_1G00036930
rosa_rugosa	Rorug05G0210300
rosa_samantha	Rh5AG294500 Rh5BG303200 Rh5CG330200 Rh5DG311900
rosa_wichuraiana	Rw5G027310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	221
AccB1I	GGYRCC	2 cut(s)	56, 268
AccII	CGCG	2 cut(s)	621, 623
AciI	CCGC	4 cut(s)	498, 535, 619, 623
AclWI	GGATC	4 cut(s)	204, 1018, 1254, 1391
AcoI	YGGCCR	1 cut(s)	698
AcsI	RAATTY	1 cut(s)	1265
AcuI	CTGAAG	1 cut(s)	1208
AcvI	CACGTG	1 cut(s)	478
AcyI	GRCGYC	1 cut(s)	1103
AfaI	GTAC	1 cut(s)	1226
AfiI	CCNNNNNNNGG	2 cut(s)	625, 821
AflIII	ACRYGT	2 cut(s)	477, 711
AgsI	TTSAA	9 cut(s)	175, 214, 304, 436, 458, 833, 1231, 1312, 1468
AjnI	CCWGG	4 cut(s)	271, 820, 985, 1444
AleI	CACNNNNGTG	2 cut(s)	710, 1241
Alw21I	GWGCWC	2 cut(s)	145, 618
Alw26I	GTCTC	3 cut(s)	270, 539, 741
AlwI	GGATC	4 cut(s)	204, 1018, 1254, 1391
AlwNI	CAGNNNCTG	1 cut(s)	469
AoxI	GGCC	2 cut(s)	654, 698
ApeKI	GCWGC	6 cut(s)	362, 592, 959, 968, 1137, 1345
ApoI	RAATTY	1 cut(s)	1265
AseI	ATTAAT	1 cut(s)	1089
Asp700I	GAANNNNTTC	1 cut(s)	816
AspLEI	GCGC	1 cut(s)	623
AspS9I	GGNCC	3 cut(s)	472, 558, 655
AsuHPI	GGTGA	2 cut(s)	1001, 1063
AsuNHI	GCTAGC	1 cut(s)	1145
AvaII	GGWCC	2 cut(s)	472, 558
BaeGI	GKGCMC	1 cut(s)	1238
BalI	TGGCCA	1 cut(s)	700
BanI	GGYRCC	2 cut(s)	56, 268
BanII	GRGCYC	2 cut(s)	145, 618
BauI	CACGAG	1 cut(s)	285
BbrPI	CACGTG	1 cut(s)	478
BbsI	GAAGAC	2 cut(s)	1312, 1456
Bbv12I	GWGCWC	2 cut(s)	145, 618
BbvI	GCAGC	6 cut(s)	349, 579, 946, 980, 1149, 1332
BccI	CCATC	5 cut(s)	503, 690, 797, 1091, 1446
BcgI	CGANNNNNNTGC	2 cut(s)	890, 924
BciT130I	CCWGG	4 cut(s)	273, 822, 987, 1446
BciVI	GTATCC	1 cut(s)	26
BcoDI	GTCTC	3 cut(s)	270, 539, 741
BfaI	CTAG	4 cut(s)	71, 312, 569, 1146
BfuAI	ACCTGC	1 cut(s)	221
BfuI	GTATCC	1 cut(s)	26
BisI	GCNGC	7 cut(s)	363, 499, 593, 960, 969, 1138, 1346
BlsI	GCNGC	7 cut(s)	364, 500, 594, 961, 970, 1139, 1347
BmcAI	AGTACT	1 cut(s)	1226
Bme1390I	CCNGG	4 cut(s)	273, 822, 987, 1446
Bme18I	GGWCC	2 cut(s)	472, 558
BmgT120I	GGNCC	3 cut(s)	472, 558, 655
BmiI	GGNNCC	3 cut(s)	58, 270, 559
BmrFI	CCNGG	4 cut(s)	273, 822, 987, 1446
BmsI	GCATC	3 cut(s)	641, 726, 898
BmtI	GCTAGC	1 cut(s)	1149
BoxI	GACNNNNGTC	1 cut(s)	1106
BpiI	GAAGAC	2 cut(s)	1312, 1456
BpmI	CTGGAG	2 cut(s)	294, 781
BpuEI	CTTGAG	1 cut(s)	337
BsaAI	YACGTR	3 cut(s)	478, 712, 1243
BsaHI	GRCGYC	1 cut(s)	1103
BsaI	GGTCTC	1 cut(s)	741
BsaJI	CCNNGG	3 cut(s)	720, 1082, 1445
Bsc4I	CCNNNNNNNGG	2 cut(s)	625, 821
Bse1I	ACTGG	4 cut(s)	58, 67, 1106, 1222
Bse3DI	GCAATG	5 cut(s)	429, 455, 732, 855, 969
BseBI	CCWGG	4 cut(s)	273, 822, 987, 1446
BseDI	CCNNGG	3 cut(s)	720, 1082, 1445
BseGI	GGATG	2 cut(s)	514, 701
BseLI	CCNNNNNNNGG	2 cut(s)	625, 821
BseMI	GCAATG	5 cut(s)	429, 455, 732, 855, 969
BseMII	CTCAG	4 cut(s)	89, 179, 264, 1264
BseNI	ACTGG	4 cut(s)	58, 67, 1106, 1222
BseRI	GAGGAG	1 cut(s)	641
BseSI	GKGCMC	1 cut(s)	1238
BseXI	GCAGC	6 cut(s)	349, 579, 946, 980, 1149, 1332
Bsh1236I	CGCG	2 cut(s)	621, 623
BshFI	GGCC	2 cut(s)	656, 700
BshNI	GGYRCC	2 cut(s)	56, 268
BsiHKAI	GWGCWC	2 cut(s)	145, 618
BslFI	GGGAC	3 cut(s)	571, 1114, 1430
BslI	CCNNNNNNNGG	2 cut(s)	625, 821
BsmAI	GTCTC	3 cut(s)	270, 539, 741
BsmFI	GGGAC	3 cut(s)	571, 1114, 1430
BsmI	GAATGC	1 cut(s)	952
BsnI	GGCC	2 cut(s)	656, 700
Bso31I	GGTCTC	1 cut(s)	741
Bsp1286I	GDGCHC	3 cut(s)	145, 618, 1238
Bsp143I	GATC	4 cut(s)	196, 1010, 1246, 1396
Bsp19I	CCATGG	1 cut(s)	1082
BspACI	CCGC	4 cut(s)	498, 535, 619, 623
BspANI	GGCC	2 cut(s)	656, 700
BspCNI	CTCAG	4 cut(s)	90, 178, 263, 1263
BspFNI	CGCG	2 cut(s)	621, 623
BspLI	GGNNCC	3 cut(s)	58, 270, 559
BspMI	ACCTGC	1 cut(s)	221
BspOI	GCTAGC	1 cut(s)	1149
BspPI	GGATC	4 cut(s)	204, 1018, 1254, 1391
BspT107I	GGYRCC	2 cut(s)	56, 268
BspTNI	GGTCTC	1 cut(s)	741
BsrDI	GCAATG	5 cut(s)	429, 455, 732, 855, 969
BsrI	ACTGG	4 cut(s)	58, 67, 1106, 1222
BssECI	CCNNGG	3 cut(s)	720, 1082, 1445
BssMI	GATC	4 cut(s)	196, 1010, 1246, 1396
BssNI	GRCGYC	1 cut(s)	1103
BssSI	CACGAG	1 cut(s)	285
BssT1I	CCWWGG	2 cut(s)	720, 1082
Bst2BI	CACGAG	1 cut(s)	285
Bst2UI	CCWGG	4 cut(s)	273, 822, 987, 1446
Bst4CI	ACNGT	3 cut(s)	265, 1168, 1182
Bst6I	CTCTTC	1 cut(s)	318
BstACI	GRCGYC	1 cut(s)	1103
BstAPI	GCANNNNNTGC	2 cut(s)	956, 974
BstBAI	YACGTR	3 cut(s)	478, 712, 1243
BstC8I	GCNNGC	1 cut(s)	1147
BstDEI	CTNAG	6 cut(s)	98, 137, 165, 250, 358, 1250
BstDSI	CCRYGG	1 cut(s)	1082
BstF5I	GGATG	2 cut(s)	514, 701
BstFNI	CGCG	2 cut(s)	621, 623
BstHHI	GCGC	1 cut(s)	623
BstKTI	GATC	4 cut(s)	199, 1013, 1249, 1399
BstMAI	GTCTC	3 cut(s)	270, 539, 741
BstMBI	GATC	4 cut(s)	196, 1010, 1246, 1396
BstMWI	GCNNNNNNNGC	7 cut(s)	629, 956, 965, 968, 974, 1146, 1285
BstNI	CCWGG	4 cut(s)	273, 822, 987, 1446
BstNSI	RCATGY	1 cut(s)	431
BstPAI	GACNNNNGTC	1 cut(s)	1106
BstSCI	CCNGG	4 cut(s)	271, 820, 985, 1444
BstSLI	GKGCMC	1 cut(s)	1238
BstUI	CGCG	2 cut(s)	621, 623
BstV1I	GCAGC	6 cut(s)	349, 579, 946, 980, 1149, 1332
BstV2I	GAAGAC	2 cut(s)	1312, 1456
BstX2I	RGATCY	2 cut(s)	196, 1246
BstXI	CCANNNNNNTGG	2 cut(s)	727, 1445
BstYI	RGATCY	2 cut(s)	196, 1246
BsuI	GTATCC	1 cut(s)	26
BsuRI	GGCC	2 cut(s)	656, 700
BtgI	CCRYGG	1 cut(s)	1082
BtsCI	GGATG	2 cut(s)	514, 701
BtsI	GCAGTG	1 cut(s)	1362
BtsIMutI	CAGTG	3 cut(s)	51, 60, 1362
BveI	ACCTGC	1 cut(s)	221
Cac8I	GCNNGC	1 cut(s)	1147
CaiI	CAGNNNCTG	1 cut(s)	469
CfoI	GCGC	1 cut(s)	623
Cfr13I	GGNCC	3 cut(s)	472, 558, 655
CseI	GACGC	1 cut(s)	1111
CsiI	ACCWGGT	1 cut(s)	985
Csp6I	GTAC	1 cut(s)	1225
CviAII	CATG	7 cut(s)	428, 538, 937, 974, 1083, 1141, 1162
CviQI	GTAC	1 cut(s)	1225
DdeI	CTNAG	6 cut(s)	98, 137, 165, 250, 358, 1250
DpnI	GATC	4 cut(s)	198, 1012, 1248, 1398
DpnII	GATC	4 cut(s)	196, 1010, 1246, 1396
EaeI	YGGCCR	1 cut(s)	698
Eam1104I	CTCTTC	1 cut(s)	318
EarI	CTCTTC	1 cut(s)	318
Ecl136II	GAGCTC	2 cut(s)	143, 616
Eco130I	CCWWGG	2 cut(s)	720, 1082
Eco24I	GRGCYC	2 cut(s)	145, 618
Eco31I	GGTCTC	1 cut(s)	741
Eco47I	GGWCC	2 cut(s)	472, 558
Eco53kI	GAGCTC	2 cut(s)	143, 616
Eco57I	CTGAAG	1 cut(s)	1208
Eco72I	CACGTG	1 cut(s)	478
EcoICRI	GAGCTC	2 cut(s)	143, 616
EcoRII	CCWGG	4 cut(s)	271, 820, 985, 1444
EcoT14I	CCWWGG	2 cut(s)	720, 1082
EcoT22I	ATGCAT	1 cut(s)	429
EcoT38I	GRGCYC	2 cut(s)	145, 618
ErhI	CCWWGG	2 cut(s)	720, 1082
FaeI	CATG	7 cut(s)	431, 541, 940, 977, 1086, 1144, 1165
FaqI	GGGAC	3 cut(s)	571, 1114, 1430
FatI	CATG	7 cut(s)	427, 537, 936, 973, 1082, 1140, 1161
Fnu4HI	GCNGC	7 cut(s)	363, 499, 593, 960, 969, 1138, 1346
FokI	GGATG	2 cut(s)	521, 708
FriOI	GRGCYC	2 cut(s)	145, 618
Fsp4HI	GCNGC	7 cut(s)	363, 499, 593, 960, 969, 1138, 1346
FspBI	CTAG	4 cut(s)	71, 312, 569, 1146
GlaI	GCGC	1 cut(s)	622
GluI	GCNGC	7 cut(s)	363, 499, 593, 960, 969, 1138, 1346
GsuI	CTGGAG	2 cut(s)	294, 781
HaeIII	GGCC	2 cut(s)	656, 700
HgaI	GACGC	1 cut(s)	1111
HhaI	GCGC	1 cut(s)	623
Hin1I	GRCGYC	1 cut(s)	1103
Hin1II	CATG	7 cut(s)	431, 541, 940, 977, 1086, 1144, 1165
Hin6I	GCGC	1 cut(s)	621
HinP1I	GCGC	1 cut(s)	621
HindIII	AAGCTT	1 cut(s)	122
HinfI	GANTC	6 cut(s)	308, 334, 672, 837, 1059, 1317
HphI	GGTGA	2 cut(s)	1001, 1063
Hpy166II	GTNNAC	4 cut(s)	355, 475, 483, 717
Hpy188I	TCNGA	4 cut(s)	253, 637, 842, 1401
Hpy188III	TCNNGA	4 cut(s)	146, 287, 1049, 1186
Hpy8I	GTNNAC	4 cut(s)	355, 475, 483, 717
HpyAV	CCTTC	6 cut(s)	29, 140, 187, 208, 827, 1183
HpyCH4III	ACNGT	3 cut(s)	265, 1168, 1182
HpyCH4IV	ACGT	4 cut(s)	477, 711, 1242, 1461
HpyF10VI	GCNNNNNNNGC	7 cut(s)	629, 956, 965, 968, 974, 1146, 1285
HpyF3I	CTNAG	6 cut(s)	98, 137, 165, 250, 358, 1250
HpySE526I	ACGT	4 cut(s)	477, 711, 1242, 1461
Hsp92I	GRCGYC	1 cut(s)	1103
Hsp92II	CATG	7 cut(s)	431, 541, 940, 977, 1086, 1144, 1165
HspAI	GCGC	1 cut(s)	621
Kzo9I	GATC	4 cut(s)	196, 1010, 1246, 1396
LmnI	GCTCC	5 cut(s)	148, 419, 600, 621, 1285
Lsp1109I	GCAGC	6 cut(s)	349, 579, 946, 980, 1149, 1332
LweI	GCATC	3 cut(s)	641, 726, 898
MabI	ACCWGGT	1 cut(s)	985
MaeI	CTAG	4 cut(s)	71, 312, 569, 1146
MaeII	ACGT	4 cut(s)	477, 711, 1242, 1461
MaeIII	GTNAC	2 cut(s)	49, 1172
MalI	GATC	4 cut(s)	198, 1012, 1248, 1398
MboI	GATC	4 cut(s)	196, 1010, 1246, 1396
MboII	GAAGA	9 cut(s)	103, 125, 335, 1037, 1060, 1300, 1312, 1433, 1456
MfeI	CAATTG	2 cut(s)	684, 912
MflI	RGATCY	2 cut(s)	196, 1246
MhlI	GDGCHC	3 cut(s)	145, 618, 1238
MlsI	TGGCCA	1 cut(s)	700
MluCI	AATT	8 cut(s)	186, 458, 684, 881, 912, 996, 1265, 1411
MluNI	TGGCCA	1 cut(s)	700
MlyI	GAGTC	3 cut(s)	343, 666, 1326
MnlI	CCTC	9 cut(s)	93, 283, 312, 619, 622, 668, 1192, 1269, 1369
Mox20I	TGGCCA	1 cut(s)	700
Mph1103I	ATGCAT	1 cut(s)	429
MroXI	GAANNNNTTC	1 cut(s)	816
MscI	TGGCCA	1 cut(s)	700
MseI	TTAA	6 cut(s)	117, 884, 1031, 1089, 1125, 1206
MslI	CAYNNNNRTG	5 cut(s)	342, 710, 941, 1053, 1241
Msp20I	TGGCCA	1 cut(s)	700
MspA1I	CMGCKG	1 cut(s)	932
MspR9I	CCNGG	4 cut(s)	273, 822, 987, 1446
MunI	CAATTG	2 cut(s)	684, 912
Mva1269I	GAATGC	1 cut(s)	952
MvaI	CCWGG	4 cut(s)	273, 822, 987, 1446
MvnI	CGCG	2 cut(s)	621, 623
MwoI	GCNNNNNNNGC	7 cut(s)	629, 956, 965, 968, 974, 1146, 1285
NcoI	CCATGG	1 cut(s)	1082
NdeII	GATC	4 cut(s)	196, 1010, 1246, 1396
NheI	GCTAGC	1 cut(s)	1145
NlaIII	CATG	7 cut(s)	431, 541, 940, 977, 1086, 1144, 1165
NlaIV	GGNNCC	3 cut(s)	58, 270, 559
NmuCI	GTSAC	1 cut(s)	49
NsiI	ATGCAT	1 cut(s)	429
NspI	RCATGY	1 cut(s)	431
OliI	CACNNNNGTG	2 cut(s)	710, 1241
PctI	GAATGC	1 cut(s)	952
PdmI	GAANNNNTTC	1 cut(s)	816
PfeI	GAWTC	3 cut(s)	308, 837, 1059
PkrI	GCNGC	7 cut(s)	364, 500, 594, 961, 970, 1139, 1347
PleI	GAGTC	3 cut(s)	342, 666, 1325
PmaCI	CACGTG	1 cut(s)	478
PmlI	CACGTG	1 cut(s)	478
PpsI	GAGTC	3 cut(s)	342, 666, 1325
Ppu21I	YACGTR	3 cut(s)	478, 712, 1243
PshAI	GACNNNNGTC	1 cut(s)	1106
PshBI	ATTAAT	1 cut(s)	1089
Psp124BI	GAGCTC	2 cut(s)	145, 618
Psp6I	CCWGG	4 cut(s)	271, 820, 985, 1444
PspCI	CACGTG	1 cut(s)	478
PspGI	CCWGG	4 cut(s)	271, 820, 985, 1444
PspN4I	GGNNCC	3 cut(s)	58, 270, 559
PspPI	GGNCC	3 cut(s)	472, 558, 655
PstNI	CAGNNNCTG	1 cut(s)	469
PsuI	RGATCY	2 cut(s)	196, 1246
PvuII	CAGCTG	1 cut(s)	932
RsaI	GTAC	1 cut(s)	1226
RsaNI	GTAC	1 cut(s)	1225
RseI	CAYNNNNRTG	5 cut(s)	342, 710, 941, 1053, 1241
SacI	GAGCTC	2 cut(s)	145, 618
SaqAI	TTAA	6 cut(s)	117, 884, 1031, 1089, 1125, 1206
SatI	GCNGC	7 cut(s)	363, 499, 593, 960, 969, 1138, 1346
Sau3AI	GATC	4 cut(s)	196, 1010, 1246, 1396
Sau96I	GGNCC	3 cut(s)	472, 558, 655
ScaI	AGTACT	1 cut(s)	1226
SchI	GAGTC	3 cut(s)	343, 666, 1326
ScrFI	CCNGG	4 cut(s)	273, 822, 987, 1446
SduI	GDGCHC	3 cut(s)	145, 618, 1238
SexAI	ACCWGGT	1 cut(s)	985
SfaNI	GCATC	3 cut(s)	641, 726, 898
SinI	GGWCC	2 cut(s)	472, 558
SmiMI	CAYNNNNRTG	5 cut(s)	342, 710, 941, 1053, 1241
SmlI	CTYRAG	1 cut(s)	316
SmoI	CTYRAG	1 cut(s)	316
Sse9I	AATT	8 cut(s)	186, 458, 684, 881, 912, 996, 1265, 1411
SsiI	CCGC	4 cut(s)	498, 535, 619, 623
SspMI	CTAG	4 cut(s)	71, 312, 569, 1146
SstI	GAGCTC	2 cut(s)	145, 618
StyD4I	CCNGG	4 cut(s)	271, 820, 985, 1444
StyI	CCWWGG	2 cut(s)	720, 1082
TaaI	ACNGT	3 cut(s)	265, 1168, 1182
TaiI	ACGT	4 cut(s)	480, 714, 1245, 1464
TaqI	TCGA	2 cut(s)	576, 1323
TasI	AATT	8 cut(s)	186, 458, 684, 881, 912, 996, 1265, 1411
TatI	WGTACW	1 cut(s)	1224
TauI	GCSGC	1 cut(s)	501
TfiI	GAWTC	3 cut(s)	308, 837, 1059
Tru1I	TTAA	6 cut(s)	117, 884, 1031, 1089, 1125, 1206
Tru9I	TTAA	6 cut(s)	117, 884, 1031, 1089, 1125, 1206
TscAI	CASTG	3 cut(s)	58, 67, 1362
TseFI	GTSAC	1 cut(s)	49
TseI	GCWGC	6 cut(s)	362, 592, 959, 968, 1137, 1345
Tsp45I	GTSAC	1 cut(s)	49
TspDTI	ATGAA	6 cut(s)	16, 251, 396, 597, 1346, 1445
TspGWI	ACGGA	2 cut(s)	1052, 1160
TspRI	CASTG	3 cut(s)	58, 67, 1362
VpaK11BI	GGWCC	2 cut(s)	472, 558
VspI	ATTAAT	1 cut(s)	1089
XapI	RAATTY	1 cut(s)	1265
XceI	RCATGY	1 cut(s)	431
XmnI	GAANNNNTTC	1 cut(s)	816
XspI	CTAG	4 cut(s)	71, 312, 569, 1146
ZrmI	AGTACT	1 cut(s)	1226
Zsp2I	ATGCAT	1 cut(s)	429

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.