Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0006104

Physical Location & Seq

Genomic Coordinates Reverse (-)

40854311 .. 40861681

Gene Structure 7371 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG294500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1473 bp

ATGGAGTTGGAAAGTTTACTCGACTTCATTAAGAACAAGAACATTTTAGTCACTGGTGCCACTGGATTTCTAGCAAAGATTTTTGTGGAGAAGATACTGAGGGTCCAACCGAATGTGAAGAAGCTCTATCTACTTTTAAGAGCTCCAGATACAAAAACAGCTACACAGCGTTTGCATAATGAGATAATTGGGAAGGACCTGTTCAGAGTGTTGAGGGAGAAATGGGGAGCAAGAATGAACTCGATCATGTCGGAAAAGCTGACGGTGGTGCCTGGAGACATTTCAAAAGAGGATTTGGGGTTGCAGGATTCTGATTTGAGGGAAGAGATTTTGAGTCAAGTAGATGTAATTGTAAACTTAGCTGCAACAACTAATTTCGATGAAAGATATGATGTTGCAATTGGTCTCAACACCATGGGCGCCAAGTATGTTTTGAGCTTTGCTAAGCTTTGTGTTAAGTTAAAGGTGCTGGTACACATCTCCACCGCGTATGTTTGGGGGGAGAAGGAAGGACTTCTACTAGAACACCCCTGCCTCATGGGGCAGTCTCTCAACGGGACCCCTGGATTAGACATCGAGACCGAAATCCGGATCGCCAACGAAGAAGTTAGACGTCTTCGGTCAGAGCATGCTTCAGAAGCAGCTATCACTCTTGCCCTCAAAGACTTTGGCCTCAAAAGAGCAAGTGTATATGGATGGCCAAACACTTATGTGTTCACCAAGGCAATGGGAGAGATGCTAATAGGAGAGCTCAGAGGAAATCTACCGGTTGTCATCCTACGCCCTACCATCATAACCAGCACTTACAAAGAACCTTTTCCTGGTTGGGTTGAAGGCATCAGAACCATTGATAGCGTAGCAGTTGGTTATGGTAAAGGAAAATTAACATTCTTTCTCTGCGACGTTGAGGCAATTGTTGATATCGTACCAGCAGACATGGTGGTGAATGCAATAATAGCAGCAATGGCGGCTCATGCAAATGAACCTGGTGAAGTAATTTATCAAGTGGGTTCTTCTGTGAGCAACCCGGTCAAATACAATGATCTTCACGATTACGGTTTTCGTTACTTCACCAGAAAACCTTGGATTAATAAAGATGGAAAGCCAGTCACGGTTAAAAAGGTCACAGTTATGAGCAGTATGGATAGCTTTCACAGATACATGACCCTCCGTTACTTGCTATTCTTAAAGGGATTGGAAGTGGTGAATATGGCATTTTGTCAGTACTTCGAAGGCACATATACTAATCTCAGTAGAAAGATCAAATTTGTGATGAGATTGGTGGAACTATACAGGCCGTATTTGTTCTTCAAAGGAGTTTTTGATGATATGAACACGGAGAAGTTGCGAATGGCAGTGAAAGAGACCACCACAGAGGCCGATATGTTTTACTTTGATCCCAAGATCATAGATTGGGAAGACTACTTTATGAATATACATATTGTTGGGGTTGTCAAATACGTGTTTAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006139 GO:0006163 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009150 GO:0009259 GO:0009698 GO:0009699 GO:0009987 GO:0010025 GO:0010166 GO:0010345 GO:0012505 GO:0016491 GO:0016620 GO:0016903 GO:0019438 GO:0019637 GO:0019693 GO:0019748 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035336 GO:0035337 GO:0035383 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0046483 GO:0051186 GO:0055086 GO:0055114 GO:0071704 GO:0072521 GO:0080019 GO:1901135 GO:1901360 GO:1901362 GO:1901564 GO:1901568 GO:1901570 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00073 ko04146 ko04212 map00073 map04146 map04212

Pfam Domains

Protein Families

NAD_binding_4 Sterile

Protein Analysis

490

Amino Acids

55.73

Weight (kDa)

8.09

Isoelectric Point (pI)

29.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	15 - 171	1.1e-06	NAD dependent epimerase/dehydratase family
NAD_binding_4	PF07993	17 - 318	6.2e-74	Male sterility protein
Sterile	PF03015	392 - 490	2.5e-18	Male sterility protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006104)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44540 AT3G44540 AT3G44540 AT4G33790 AT4G33790 AT5G22500 AT5G22500
fragaria_vesca	FvH4_3g24571 FvH4_3g24571 FvH4_3g24571 FvH4_3g24571 FvH4_3g24571
malus_domestica	MD03G1141800.v1.1 MD11G1161800.v1.1
prunus_persica	Prupe.6G126900_v2.0.a1
pyrus_communis	pycom03g09660 pycom11g13340
rosa_chinensis	RchiOBHm_Chr5g0044101
rosa_laevigata	RLG00000034241
rosa_multiflora	Rmu_sc0000939.1_g000010
rosa_roxburghii	Rroxscaffold_1G00036930
rosa_rugosa	Rorug05G0210300
rosa_samantha	Rh5AG294500 Rh5BG303200 Rh5CG330200 Rh5DG311900
rosa_wichuraiana	Rw5G027310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	616
AccB1I	GGYRCC	3 cut(s)	56, 268, 419
AccII	CGCG	1 cut(s)	488
AccIII	TCCGGA	1 cut(s)	588
AciI	CCGC	2 cut(s)	486, 968
AclWI	GGATC	2 cut(s)	599, 1391
AcoI	YGGCCR	1 cut(s)	698
AcsI	RAATTY	1 cut(s)	1265
AcuI	CTGAAG	1 cut(s)	618
AcyI	GRCGYC	2 cut(s)	420, 613
AfaI	GTAC	3 cut(s)	474, 927, 1226
AfiI	CCNNNNNNNGG	2 cut(s)	588, 821
AflIII	ACRYGT	1 cut(s)	1461
AgeI	ACCGGT	1 cut(s)	766
AgsI	TTSAA	3 cut(s)	285, 833, 1312
AjnI	CCWGG	4 cut(s)	271, 562, 820, 985
AleI	CACNNNNGTG	1 cut(s)	710
Alw21I	GWGCWC	2 cut(s)	145, 753
Alw26I	GTCTC	5 cut(s)	270, 410, 552, 572, 1358
AlwI	GGATC	2 cut(s)	599, 1391
Aor13HI	TCCGGA	1 cut(s)	588
AoxI	GGCC	4 cut(s)	670, 698, 1295, 1377
ApeKI	GCWGC	3 cut(s)	362, 641, 959
ApoI	RAATTY	1 cut(s)	1265
AseI	ATTAAT	1 cut(s)	1089
AsiGI	ACCGGT	1 cut(s)	766
Asp700I	GAANNNNTTC	2 cut(s)	513, 816
AspLEI	GCGC	1 cut(s)	422
AspS9I	GGNCC	3 cut(s)	103, 196, 558
AsuC2I	CCSGG	1 cut(s)	1028
AsuHPI	GGTGA	5 cut(s)	709, 955, 1001, 1063, 1216
AsuII	TTCGAA	1 cut(s)	1230
AvaII	GGWCC	3 cut(s)	103, 196, 558
BalI	TGGCCA	1 cut(s)	700
BanI	GGYRCC	3 cut(s)	56, 268, 419
BanII	GRGCYC	2 cut(s)	145, 753
BarI	GAAGNNNNNNTAC	4 cut(s)	501, 533, 1292, 1324
BbsI	GAAGAC	2 cut(s)	608, 1425
Bbv12I	GWGCWC	2 cut(s)	145, 753
BbvI	GCAGC	3 cut(s)	349, 653, 971
BccI	CCATC	3 cut(s)	690, 797, 1091
BceAI	ACGGC	1 cut(s)	1282
BciT130I	CCWGG	4 cut(s)	273, 564, 822, 987
BcnI	CCSGG	1 cut(s)	1028
BcoDI	GTCTC	5 cut(s)	270, 410, 552, 572, 1358
BfaI	CTAG	2 cut(s)	71, 521
BfoI	RGCGCY	1 cut(s)	423
BisI	GCNGC	4 cut(s)	363, 642, 960, 969
BlpI	GCTNAGC	1 cut(s)	444
BlsI	GCNGC	4 cut(s)	364, 643, 961, 970
BmcAI	AGTACT	1 cut(s)	1226
Bme1390I	CCNGG	5 cut(s)	273, 564, 822, 987, 1028
Bme18I	GGWCC	3 cut(s)	103, 196, 558
BmgT120I	GGNCC	3 cut(s)	103, 196, 558
BmiI	GGNNCC	6 cut(s)	58, 104, 270, 421, 559, 560
BmrFI	CCNGG	5 cut(s)	273, 564, 822, 987, 1028
BmsI	GCATC	2 cut(s)	726, 846
BpiI	GAAGAC	2 cut(s)	608, 1425
BpmI	CTGGAG	2 cut(s)	129, 294
Bpu1102I	GCTNAGC	1 cut(s)	444
Bpu14I	TTCGAA	1 cut(s)	1230
BpuMI	CCSGG	1 cut(s)	1028
BsaAI	YACGTR	1 cut(s)	1462
BsaHI	GRCGYC	2 cut(s)	420, 613
BsaI	GGTCTC	3 cut(s)	410, 572, 1358
BsaJI	CCNNGG	4 cut(s)	414, 562, 720, 1082
BsaWI	WCCGGW	2 cut(s)	588, 766
Bsc4I	CCNNNNNNNGG	2 cut(s)	588, 821
Bse118I	RCCGGY	1 cut(s)	766
Bse1I	ACTGG	3 cut(s)	58, 67, 1106
Bse3DI	GCAATG	2 cut(s)	732, 969
BseAI	TCCGGA	1 cut(s)	588
BseBI	CCWGG	4 cut(s)	273, 564, 822, 987
BseDI	CCNNGG	4 cut(s)	414, 562, 720, 1082
BseGI	GGATG	2 cut(s)	701, 774
BseLI	CCNNNNNNNGG	2 cut(s)	588, 821
BseMI	GCAATG	2 cut(s)	732, 969
BseMII	CTCAG	3 cut(s)	89, 766, 1264
BseNI	ACTGG	3 cut(s)	58, 67, 1106
BseXI	GCAGC	3 cut(s)	349, 653, 971
Bsh1236I	CGCG	1 cut(s)	488
BshFI	GGCC	4 cut(s)	672, 700, 1297, 1379
BshNI	GGYRCC	3 cut(s)	56, 268, 419
BshTI	ACCGGT	1 cut(s)	766
BsiHKAI	GWGCWC	2 cut(s)	145, 753
BsiSI	CCGG	3 cut(s)	589, 767, 1028
BslFI	GGGAC	1 cut(s)	571
BslI	CCNNNNNNNGG	2 cut(s)	588, 821
BsmAI	GTCTC	5 cut(s)	270, 410, 552, 572, 1358
BsmFI	GGGAC	1 cut(s)	571
BsmI	GAATGC	1 cut(s)	952
BsnI	GGCC	4 cut(s)	672, 700, 1297, 1379
Bso31I	GGTCTC	3 cut(s)	410, 572, 1358
Bsp119I	TTCGAA	1 cut(s)	1230
Bsp1286I	GDGCHC	2 cut(s)	145, 753
Bsp13I	TCCGGA	1 cut(s)	588
Bsp143I	GATC	6 cut(s)	243, 591, 1042, 1260, 1396, 1404
Bsp1720I	GCTNAGC	1 cut(s)	444
Bsp19I	CCATGG	1 cut(s)	414
BspACI	CCGC	2 cut(s)	486, 968
BspANI	GGCC	4 cut(s)	672, 700, 1297, 1379
BspCNI	CTCAG	3 cut(s)	90, 765, 1263
BspEI	TCCGGA	1 cut(s)	588
BspFNI	CGCG	1 cut(s)	488
BspLI	GGNNCC	6 cut(s)	58, 104, 270, 421, 559, 560
BspPI	GGATC	2 cut(s)	599, 1391
BspT104I	TTCGAA	1 cut(s)	1230
BspT107I	GGYRCC	3 cut(s)	56, 268, 419
BspTNI	GGTCTC	3 cut(s)	410, 572, 1358
BsrDI	GCAATG	2 cut(s)	732, 969
BsrFI	RCCGGY	1 cut(s)	766
BsrI	ACTGG	3 cut(s)	58, 67, 1106
BssAI	RCCGGY	1 cut(s)	766
BssECI	CCNNGG	4 cut(s)	414, 562, 720, 1082
BssMI	GATC	6 cut(s)	243, 591, 1042, 1260, 1396, 1404
BssNI	GRCGYC	2 cut(s)	420, 613
BssT1I	CCWWGG	3 cut(s)	414, 720, 1082
Bst2UI	CCWGG	4 cut(s)	273, 564, 822, 987
Bst4CI	ACNGT	4 cut(s)	265, 1058, 1114, 1129
Bst6I	CTCTTC	1 cut(s)	318
BstACI	GRCGYC	2 cut(s)	420, 613
BstBAI	YACGTR	1 cut(s)	1462
BstBI	TTCGAA	1 cut(s)	1230
BstC8I	GCNNGC	1 cut(s)	630
BstDEI	CTNAG	5 cut(s)	98, 358, 444, 752, 1250
BstDSI	CCRYGG	1 cut(s)	414
BstF5I	GGATG	2 cut(s)	701, 774
BstFNI	CGCG	1 cut(s)	488
BstH2I	RGCGCY	1 cut(s)	423
BstHHI	GCGC	1 cut(s)	422
BstKTI	GATC	6 cut(s)	246, 594, 1045, 1263, 1399, 1407
BstMAI	GTCTC	5 cut(s)	270, 410, 552, 572, 1358
BstMBI	GATC	6 cut(s)	243, 591, 1042, 1260, 1396, 1404
BstMWI	GCNNNNNNNGC	5 cut(s)	638, 956, 965, 968, 974
BstNI	CCWGG	4 cut(s)	273, 564, 822, 987
BstNSI	RCATGY	1 cut(s)	632
BstSCI	CCNGG	5 cut(s)	271, 562, 820, 985, 1026
BstUI	CGCG	1 cut(s)	488
BstV1I	GCAGC	3 cut(s)	349, 653, 971
BstV2I	GAAGAC	2 cut(s)	608, 1425
BstXI	CCANNNNNNTGG	1 cut(s)	727
BsuRI	GGCC	4 cut(s)	672, 700, 1297, 1379
BtgI	CCRYGG	1 cut(s)	414
BtsCI	GGATG	2 cut(s)	701, 774
BtsI	GCAGTG	1 cut(s)	1362
BtsIMutI	CAGTG	3 cut(s)	51, 60, 1362
Cac8I	GCNNGC	1 cut(s)	630
CfoI	GCGC	1 cut(s)	422
Cfr10I	RCCGGY	1 cut(s)	766
Cfr13I	GGNCC	3 cut(s)	103, 196, 558
CsiI	ACCWGGT	1 cut(s)	985
Csp6I	GTAC	3 cut(s)	473, 926, 1225
CspAI	ACCGGT	1 cut(s)	766
CviAII	CATG	7 cut(s)	247, 415, 538, 629, 937, 974, 1162
CviQI	GTAC	3 cut(s)	473, 926, 1225
DdeI	CTNAG	5 cut(s)	98, 358, 444, 752, 1250
DinI	GGCGCC	1 cut(s)	421
DpnI	GATC	6 cut(s)	245, 593, 1044, 1262, 1398, 1406
DpnII	GATC	6 cut(s)	243, 591, 1042, 1260, 1396, 1404
DraI	TTTAAA	1 cut(s)	1468
EaeI	YGGCCR	1 cut(s)	698
Eam1104I	CTCTTC	1 cut(s)	318
EarI	CTCTTC	1 cut(s)	318
Ecl136II	GAGCTC	2 cut(s)	143, 751
Eco130I	CCWWGG	3 cut(s)	414, 720, 1082
Eco24I	GRGCYC	2 cut(s)	145, 753
Eco31I	GGTCTC	3 cut(s)	410, 572, 1358
Eco32I	GATATC	1 cut(s)	922
Eco47I	GGWCC	3 cut(s)	103, 196, 558
Eco53kI	GAGCTC	2 cut(s)	143, 751
Eco57I	CTGAAG	1 cut(s)	618
EcoICRI	GAGCTC	2 cut(s)	143, 751
EcoO109I	RGGNCCY	2 cut(s)	196, 558
EcoRII	CCWGG	4 cut(s)	271, 562, 820, 985
EcoRV	GATATC	1 cut(s)	922
EcoT14I	CCWWGG	3 cut(s)	414, 720, 1082
EcoT38I	GRGCYC	2 cut(s)	145, 753
EgeI	GGCGCC	1 cut(s)	421
EheI	GGCGCC	1 cut(s)	421
ErhI	CCWWGG	3 cut(s)	414, 720, 1082
FaeI	CATG	7 cut(s)	250, 418, 541, 632, 940, 977, 1165
FaqI	GGGAC	1 cut(s)	571
FatI	CATG	7 cut(s)	246, 414, 537, 628, 936, 973, 1161
Fnu4HI	GCNGC	4 cut(s)	363, 642, 960, 969
FokI	GGATG	2 cut(s)	708, 761
FriOI	GRGCYC	2 cut(s)	145, 753
Fsp4HI	GCNGC	4 cut(s)	363, 642, 960, 969
FspBI	CTAG	2 cut(s)	71, 521
GlaI	GCGC	1 cut(s)	421
GluI	GCNGC	4 cut(s)	363, 642, 960, 969
GsuI	CTGGAG	2 cut(s)	129, 294
HaeII	RGCGCY	1 cut(s)	423
HaeIII	GGCC	4 cut(s)	672, 700, 1297, 1379
HapII	CCGG	3 cut(s)	589, 767, 1028
HhaI	GCGC	1 cut(s)	422
Hin1I	GRCGYC	2 cut(s)	420, 613
Hin1II	CATG	7 cut(s)	250, 418, 541, 632, 940, 977, 1165
Hin6I	GCGC	1 cut(s)	420
HinP1I	GCGC	1 cut(s)	420
HindIII	AAGCTT	1 cut(s)	446
HinfI	GANTC	2 cut(s)	308, 334
HpaII	CCGG	3 cut(s)	589, 767, 1028
HphI	GGTGA	5 cut(s)	709, 955, 1001, 1063, 1216
Hpy166II	GTNNAC	4 cut(s)	17, 355, 475, 717
Hpy188I	TCNGA	7 cut(s)	206, 253, 313, 625, 637, 755, 842
Hpy188III	TCNNGA	4 cut(s)	146, 577, 589, 1049
Hpy8I	GTNNAC	4 cut(s)	17, 355, 475, 717
Hpy99I	CGWCG	1 cut(s)	905
HpyAV	CCTTC	5 cut(s)	187, 499, 503, 827, 1226
HpyCH4III	ACNGT	4 cut(s)	265, 1058, 1114, 1129
HpyCH4IV	ACGT	3 cut(s)	613, 903, 1461
HpyCH4V	TGCA	6 cut(s)	175, 304, 365, 398, 950, 977
HpyF10VI	GCNNNNNNNGC	5 cut(s)	638, 956, 965, 968, 974
HpyF3I	CTNAG	5 cut(s)	98, 358, 444, 752, 1250
HpySE526I	ACGT	3 cut(s)	613, 903, 1461
Hsp92I	GRCGYC	2 cut(s)	420, 613
Hsp92II	CATG	7 cut(s)	250, 418, 541, 632, 940, 977, 1165
HspAI	GCGC	1 cut(s)	420
KasI	GGCGCC	1 cut(s)	419
KflI	GGGWCCC	1 cut(s)	558
Kpn2I	TCCGGA	1 cut(s)	588
Kzo9I	GATC	6 cut(s)	243, 591, 1042, 1260, 1396, 1404
LmnI	GCTCC	2 cut(s)	148, 227
Lsp1109I	GCAGC	3 cut(s)	349, 653, 971
LweI	GCATC	2 cut(s)	726, 846
MabI	ACCWGGT	1 cut(s)	985
MaeI	CTAG	2 cut(s)	71, 521
MaeII	ACGT	3 cut(s)	613, 903, 1461
MaeIII	GTNAC	5 cut(s)	49, 1064, 1108, 1123, 1172
MalI	GATC	6 cut(s)	245, 593, 1044, 1262, 1398, 1406
MboI	GATC	6 cut(s)	243, 591, 1042, 1260, 1396, 1404
MboII	GAAGA	9 cut(s)	103, 130, 335, 608, 614, 1005, 1037, 1300, 1430
MfeI	CAATTG	2 cut(s)	399, 912
MhlI	GDGCHC	2 cut(s)	145, 753
MlsI	TGGCCA	1 cut(s)	700
MluCI	AATT	8 cut(s)	186, 348, 373, 399, 881, 912, 996, 1265
MluNI	TGGCCA	1 cut(s)	700
Mly113I	GGCGCC	1 cut(s)	420
MlyI	GAGTC	1 cut(s)	343
MmeI	TCCRAC	2 cut(s)	130, 231
Mox20I	TGGCCA	1 cut(s)	700
MroI	TCCGGA	1 cut(s)	588
MroXI	GAANNNNTTC	2 cut(s)	513, 816
MscI	TGGCCA	1 cut(s)	700
MseI	TTAA	9 cut(s)	30, 137, 456, 461, 884, 1089, 1116, 1187, 1467
MslI	CAYNNNNRTG	4 cut(s)	710, 941, 978, 1130
Msp20I	TGGCCA	1 cut(s)	700
MspI	CCGG	3 cut(s)	589, 767, 1028
MspR9I	CCNGG	5 cut(s)	273, 564, 822, 987, 1028
MunI	CAATTG	2 cut(s)	399, 912
Mva1269I	GAATGC	1 cut(s)	952
MvaI	CCWGG	4 cut(s)	273, 564, 822, 987
MvnI	CGCG	1 cut(s)	488
MwoI	GCNNNNNNNGC	5 cut(s)	638, 956, 965, 968, 974
NarI	GGCGCC	1 cut(s)	420
NciI	CCSGG	1 cut(s)	1028
NcoI	CCATGG	1 cut(s)	414
NdeII	GATC	6 cut(s)	243, 591, 1042, 1260, 1396, 1404
NlaIII	CATG	7 cut(s)	250, 418, 541, 632, 940, 977, 1165
NlaIV	GGNNCC	6 cut(s)	58, 104, 270, 421, 559, 560
NmuCI	GTSAC	3 cut(s)	49, 1108, 1123
NspI	RCATGY	1 cut(s)	632
NspV	TTCGAA	1 cut(s)	1230
OliI	CACNNNNGTG	1 cut(s)	710
PaeI	GCATGC	1 cut(s)	632
PctI	GAATGC	1 cut(s)	952
PdmI	GAANNNNTTC	2 cut(s)	513, 816
PfeI	GAWTC	1 cut(s)	308
PinAI	ACCGGT	1 cut(s)	766
PkrI	GCNGC	4 cut(s)	364, 643, 961, 970
PleI	GAGTC	1 cut(s)	342
PluTI	GGCGCC	1 cut(s)	423
PpsI	GAGTC	1 cut(s)	342
Ppu21I	YACGTR	1 cut(s)	1462
PpuMI	RGGWCCY	2 cut(s)	196, 558
PshBI	ATTAAT	1 cut(s)	1089
Psp124BI	GAGCTC	2 cut(s)	145, 753
Psp5II	RGGWCCY	2 cut(s)	196, 558
Psp6I	CCWGG	4 cut(s)	271, 562, 820, 985
PspGI	CCWGG	4 cut(s)	271, 562, 820, 985
PspN4I	GGNNCC	6 cut(s)	58, 104, 270, 421, 559, 560
PspPI	GGNCC	3 cut(s)	103, 196, 558
PspPPI	RGGWCCY	2 cut(s)	196, 558
RsaI	GTAC	3 cut(s)	474, 927, 1226
RsaNI	GTAC	3 cut(s)	473, 926, 1225
RseI	CAYNNNNRTG	4 cut(s)	710, 941, 978, 1130
SacI	GAGCTC	2 cut(s)	145, 753
SaqAI	TTAA	9 cut(s)	30, 137, 456, 461, 884, 1089, 1116, 1187, 1467
SatI	GCNGC	4 cut(s)	363, 642, 960, 969
Sau3AI	GATC	6 cut(s)	243, 591, 1042, 1260, 1396, 1404
Sau96I	GGNCC	3 cut(s)	103, 196, 558
ScaI	AGTACT	1 cut(s)	1226
SchI	GAGTC	1 cut(s)	343
ScrFI	CCNGG	5 cut(s)	273, 564, 822, 987, 1028
SduI	GDGCHC	2 cut(s)	145, 753
SexAI	ACCWGGT	1 cut(s)	985
SfaNI	GCATC	2 cut(s)	726, 846
SfoI	GGCGCC	1 cut(s)	421
SfuI	TTCGAA	1 cut(s)	1230
SinI	GGWCC	3 cut(s)	103, 196, 558
SmiMI	CAYNNNNRTG	4 cut(s)	710, 941, 978, 1130
SphI	GCATGC	1 cut(s)	632
Sse9I	AATT	8 cut(s)	186, 348, 373, 399, 881, 912, 996, 1265
SsiI	CCGC	2 cut(s)	486, 968
SspDI	GGCGCC	1 cut(s)	419
SspMI	CTAG	2 cut(s)	71, 521
SstI	GAGCTC	2 cut(s)	145, 753
StyD4I	CCNGG	5 cut(s)	271, 562, 820, 985, 1026
StyI	CCWWGG	3 cut(s)	414, 720, 1082
TaaI	ACNGT	4 cut(s)	265, 1058, 1114, 1129
TaiI	ACGT	3 cut(s)	616, 906, 1464
TaqI	TCGA	5 cut(s)	21, 242, 378, 576, 1230
TaqII	GACCGA	2 cut(s)	596, 609
TasI	AATT	8 cut(s)	186, 348, 373, 399, 881, 912, 996, 1265
TatI	WGTACW	1 cut(s)	1224
TauI	GCSGC	1 cut(s)	971
TfiI	GAWTC	1 cut(s)	308
Tru1I	TTAA	9 cut(s)	30, 137, 456, 461, 884, 1089, 1116, 1187, 1467
Tru9I	TTAA	9 cut(s)	30, 137, 456, 461, 884, 1089, 1116, 1187, 1467
TscAI	CASTG	3 cut(s)	58, 67, 1362
TseFI	GTSAC	3 cut(s)	49, 1108, 1123
TseI	GCWGC	3 cut(s)	362, 641, 959
Tsp45I	GTSAC	3 cut(s)	49, 1108, 1123
TspDTI	ATGAA	6 cut(s)	16, 251, 396, 996, 1346, 1445
TspGWI	ACGGA	2 cut(s)	1160, 1352
TspRI	CASTG	3 cut(s)	58, 67, 1362
VpaK11BI	GGWCC	3 cut(s)	103, 196, 558
VspI	ATTAAT	1 cut(s)	1089
XapI	RAATTY	1 cut(s)	1265
XceI	RCATGY	1 cut(s)	632
XmnI	GAANNNNTTC	2 cut(s)	513, 816
XspI	CTAG	2 cut(s)	71, 521
ZraI	GACGTC	1 cut(s)	614
ZrmI	AGTACT	1 cut(s)	1226

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.