Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0006104

Physical Location & Seq

Genomic Coordinates Forward (+)

53914915 .. 53921543

Gene Structure 6629 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00036930.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1527 bp

ATGGAGTTGGAAAGCTTACTCGACTTCATTCAGAACAAGAACATTTTAGTCACTGGTGCCACTGGATTTCTAGCAAAGATTTTTGTGGAGAAGATACTGAGGGTCCAACCGAATGTGAATAAGCTCTATCTACTTTTAAGAGCTCCAGATACAAAAACAGCTACACAGCGTTTGCATAATGAGATGATTGGGAAGGACCTGTTCAGAGTGTTGAGGGAGAAATGGGGAGCAAGAATGAACTCGATCGTGTCGGAAAAGCTGACCGTGGTGCCTGGAGACATTTCAAAAGAGGATTTGGGGTTGCAGGATTCTGATTTGAGGGAAGAGATTTTGAGTCAAGTAGATGTCATTGTAAACTTAGCTGCAACAACTAATTTCGATGAAAGATATGATGTTGCAATTGGTCTCAACACAATGGGCGCCAAGGTTTGCTTGATTGGATCCACTTATGCTCATATATTTCTGTTCAGTTATGACCTTTACGAAAATGATTCATACTGTTTTGTCTTCATGATAATTACATCAATAATTGGAACACACACCTTATGTTTGGGGGAGAAGGAAGGACTTCTACTAGAACACCCCTGCCTCATGGGGCAGTCTCTCAACGGGACCCCTGGATTAGACATCGAGACCGAAATCCGGATCGCCAACGAAGAAGTTAGACGTCTCCGGTCAGAGCATGCTTCAGAAGCAGCTGTCACTCTTGCCCTCAAAGACTTTGGCCTCAAAAGAGCAAGTGTATATGGATGGCCAAACACTTATGTGTTCACCAAGGCAATGGGAGAGATGCTAATAGGAGAGCTCAGAGGAAATCTACCGGTTGTCATCCTACGCCCTACCATCATCACCAGCACTTACAAAGAACCTTTTCCTGGTTGGGTTGAAGGCATCAGAACCATTGATAGCGTAGCAGTTGGTTATGGTAAAGGAAAATTAACATTCTTTCTCTGCGACGTTGAGGCAATTGTTGATATCGTACCAGCAGACATGGTGGTGAATGCGATAATAGCAGCAATGGCGGCTCATGCAAATGAACCTGGTGAAGTAATTTATCAAGTGGGTTCTTCTGTGAGCAACCCGGTCAAATACAATGATCTTCATGATTACGGTTTTCGTTACTTCACCAGAAAACCTTGGATTAATAAAGATGGAAAGCCAGTCACGGTTAAAAAGGTCACAGTTATGAGCAGCATGGATAGCTTTCACAGATACATGACCCTCCGTTACTTGCTATTCTTAAAGGGATTGAAACTGGTGAATATGGCATTTTGTCAGTACTTCGAAGGCACATATACTGATCTCAATAGAAAGATCAAATTTGTGATGAGATTGGTGGAACTATACAGGCCGTACTTGTTCTTCAAAGGAGTTTTTGATGATATGAACACGGAGAAGTTGCGAATGGCAGTGAAAGAGACCACCACAGAGGCCGATATGTTTTACTTTGATCCCAAGATCATAGATTGGGAAGACTACTTTATGAATATACATATTGTTGGGGTTGTCAAATACGTGTTTAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006139 GO:0006163 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009150 GO:0009259 GO:0009698 GO:0009699 GO:0009987 GO:0010025 GO:0010166 GO:0010345 GO:0012505 GO:0016491 GO:0016620 GO:0016903 GO:0019438 GO:0019637 GO:0019693 GO:0019748 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035336 GO:0035337 GO:0035383 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0046483 GO:0051186 GO:0055086 GO:0055114 GO:0071704 GO:0072521 GO:0080019 GO:1901135 GO:1901360 GO:1901362 GO:1901564 GO:1901568 GO:1901570 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00073 ko04146 ko04212 map00073 map04146 map04212

Pfam Domains

Protein Families

NAD_binding_4 Sterile

Protein Analysis

508

Amino Acids

57.77

Weight (kDa)

6.07

Isoelectric Point (pI)

30.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAD_binding_4	PF07993	17 - 144	2.2e-34	Male sterility protein
NAD_binding_4	PF07993	240 - 336	2.5e-24	Male sterility protein
Sterile	PF03015	410 - 508	1.7e-18	Male sterility protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006104)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44540 AT3G44540 AT3G44540 AT4G33790 AT4G33790 AT5G22500 AT5G22500
fragaria_vesca	FvH4_3g24571 FvH4_3g24571 FvH4_3g24571 FvH4_3g24571 FvH4_3g24571
malus_domestica	MD03G1141800.v1.1 MD11G1161800.v1.1
prunus_persica	Prupe.6G126900_v2.0.a1
pyrus_communis	pycom03g09660 pycom11g13340
rosa_chinensis	RchiOBHm_Chr5g0044101
rosa_laevigata	RLG00000034241
rosa_multiflora	Rmu_sc0000939.1_g000010
rosa_roxburghii	Rroxscaffold_1G00036930
rosa_rugosa	Rorug05G0210300
rosa_samantha	Rh5AG294500 Rh5BG303200 Rh5CG330200 Rh5DG311900
rosa_wichuraiana	Rw5G027310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	670
AccB1I	GGYRCC	3 cut(s)	56, 268, 419
AccIII	TCCGGA	1 cut(s)	642
AciI	CCGC	1 cut(s)	1022
AclWI	GGATC	4 cut(s)	435, 448, 653, 1445
AcoI	YGGCCR	1 cut(s)	752
AcsI	RAATTY	1 cut(s)	1319
AcuI	CTGAAG	1 cut(s)	672
AcyI	GRCGYC	2 cut(s)	420, 667
AfaI	GTAC	3 cut(s)	981, 1280, 1355
AfiI	CCNNNNNNNGG	2 cut(s)	642, 875
AflIII	ACRYGT	1 cut(s)	1515
AgeI	ACCGGT	1 cut(s)	820
AgsI	TTSAA	4 cut(s)	285, 887, 1252, 1366
AjnI	CCWGG	4 cut(s)	271, 616, 874, 1039
AleI	CACNNNNGTG	1 cut(s)	764
AluBI	AGCT	9 cut(s)	15, 124, 143, 161, 259, 362, 698, 805, 1203
AluI	AGCT	9 cut(s)	15, 124, 143, 161, 259, 362, 698, 805, 1203
Alw21I	GWGCWC	2 cut(s)	145, 807
Alw26I	GTCTC	6 cut(s)	270, 410, 606, 626, 674, 1412
AlwI	GGATC	4 cut(s)	435, 448, 653, 1445
Aor13HI	TCCGGA	1 cut(s)	642
AoxI	GGCC	4 cut(s)	724, 752, 1349, 1431
ApeKI	GCWGC	4 cut(s)	362, 695, 1013, 1191
ApoI	RAATTY	1 cut(s)	1319
AseI	ATTAAT	1 cut(s)	1143
AsiGI	ACCGGT	1 cut(s)	820
Asp700I	GAANNNNTTC	2 cut(s)	567, 870
AspLEI	GCGC	1 cut(s)	422
AspS9I	GGNCC	3 cut(s)	103, 196, 612
AsuC2I	CCSGG	1 cut(s)	1082
AsuHPI	GGTGA	6 cut(s)	763, 841, 1009, 1055, 1117, 1270
AsuII	TTCGAA	1 cut(s)	1284
AvaII	GGWCC	3 cut(s)	103, 196, 612
BalI	TGGCCA	1 cut(s)	754
BamHI	GGATCC	1 cut(s)	440
BanI	GGYRCC	3 cut(s)	56, 268, 419
BanII	GRGCYC	2 cut(s)	145, 807
BarI	GAAGNNNNNNTAC	4 cut(s)	555, 587, 1346, 1378
BbsI	GAAGAC	2 cut(s)	499, 1479
Bbv12I	GWGCWC	2 cut(s)	145, 807
BbvI	GCAGC	4 cut(s)	349, 707, 1025, 1203
BccI	CCATC	3 cut(s)	744, 851, 1145
BceAI	ACGGC	1 cut(s)	1336
BciT130I	CCWGG	4 cut(s)	273, 618, 876, 1041
BcnI	CCSGG	1 cut(s)	1082
BcoDI	GTCTC	6 cut(s)	270, 410, 606, 626, 674, 1412
BfaI	CTAG	2 cut(s)	71, 575
BfoI	RGCGCY	1 cut(s)	423
BisI	GCNGC	5 cut(s)	363, 696, 1014, 1023, 1192
BlsI	GCNGC	5 cut(s)	364, 697, 1015, 1024, 1193
BmcAI	AGTACT	1 cut(s)	1280
Bme1390I	CCNGG	5 cut(s)	273, 618, 876, 1041, 1082
Bme18I	GGWCC	3 cut(s)	103, 196, 612
BmgT120I	GGNCC	3 cut(s)	103, 196, 612
BmiI	GGNNCC	7 cut(s)	58, 104, 270, 421, 442, 613, 614
BmrFI	CCNGG	5 cut(s)	273, 618, 876, 1041, 1082
BmsI	GCATC	2 cut(s)	780, 900
BpiI	GAAGAC	2 cut(s)	499, 1479
BpmI	CTGGAG	2 cut(s)	129, 294
Bpu14I	TTCGAA	1 cut(s)	1284
BpuMI	CCSGG	1 cut(s)	1082
BsaAI	YACGTR	1 cut(s)	1516
BsaHI	GRCGYC	2 cut(s)	420, 667
BsaI	GGTCTC	3 cut(s)	410, 626, 1412
BsaJI	CCNNGG	5 cut(s)	264, 423, 616, 774, 1136
BsaWI	WCCGGW	3 cut(s)	642, 672, 820
Bsc4I	CCNNNNNNNGG	2 cut(s)	642, 875
Bse118I	RCCGGY	1 cut(s)	820
Bse1I	ACTGG	4 cut(s)	58, 67, 1160, 1260
Bse3DI	GCAATG	2 cut(s)	786, 1023
BseAI	TCCGGA	1 cut(s)	642
BseBI	CCWGG	4 cut(s)	273, 618, 876, 1041
BseDI	CCNNGG	5 cut(s)	264, 423, 616, 774, 1136
BseGI	GGATG	2 cut(s)	755, 828
BseLI	CCNNNNNNNGG	2 cut(s)	642, 875
BseMI	GCAATG	2 cut(s)	786, 1023
BseMII	CTCAG	2 cut(s)	89, 820
BseNI	ACTGG	4 cut(s)	58, 67, 1160, 1260
BseXI	GCAGC	4 cut(s)	349, 707, 1025, 1203
Bsh1285I	CGRYCG	1 cut(s)	246
BshFI	GGCC	4 cut(s)	726, 754, 1351, 1433
BshNI	GGYRCC	3 cut(s)	56, 268, 419
BshTI	ACCGGT	1 cut(s)	820
BsiEI	CGRYCG	1 cut(s)	246
BsiHKAI	GWGCWC	2 cut(s)	145, 807
BsiSI	CCGG	4 cut(s)	643, 673, 821, 1082
BslFI	GGGAC	1 cut(s)	625
BslI	CCNNNNNNNGG	2 cut(s)	642, 875
BsmAI	GTCTC	6 cut(s)	270, 410, 606, 626, 674, 1412
BsmBI	CGTCTC	1 cut(s)	674
BsmFI	GGGAC	1 cut(s)	625
BsmI	GAATGC	1 cut(s)	1006
BsnI	GGCC	4 cut(s)	726, 754, 1351, 1433
Bso31I	GGTCTC	3 cut(s)	410, 626, 1412
Bsp119I	TTCGAA	1 cut(s)	1284
Bsp1286I	GDGCHC	2 cut(s)	145, 807
Bsp13I	TCCGGA	1 cut(s)	642
Bsp143I	GATC	8 cut(s)	243, 440, 645, 1096, 1300, 1314, 1450, 1458
BspACI	CCGC	1 cut(s)	1022
BspANI	GGCC	4 cut(s)	726, 754, 1351, 1433
BspCNI	CTCAG	2 cut(s)	90, 819
BspEI	TCCGGA	1 cut(s)	642
BspHI	TCATGA	2 cut(s)	510, 1102
BspLI	GGNNCC	7 cut(s)	58, 104, 270, 421, 442, 613, 614
BspPI	GGATC	4 cut(s)	435, 448, 653, 1445
BspT104I	TTCGAA	1 cut(s)	1284
BspT107I	GGYRCC	3 cut(s)	56, 268, 419
BspTNI	GGTCTC	3 cut(s)	410, 626, 1412
BsrDI	GCAATG	2 cut(s)	786, 1023
BsrFI	RCCGGY	1 cut(s)	820
BsrI	ACTGG	4 cut(s)	58, 67, 1160, 1260
BssAI	RCCGGY	1 cut(s)	820
BssECI	CCNNGG	5 cut(s)	264, 423, 616, 774, 1136
BssMI	GATC	8 cut(s)	243, 440, 645, 1096, 1300, 1314, 1450, 1458
BssNI	GRCGYC	2 cut(s)	420, 667
BssT1I	CCWWGG	3 cut(s)	423, 774, 1136
Bst2UI	CCWGG	4 cut(s)	273, 618, 876, 1041
Bst4CI	ACNGT	5 cut(s)	265, 500, 1112, 1168, 1183
Bst6I	CTCTTC	1 cut(s)	318
BstACI	GRCGYC	2 cut(s)	420, 667
BstBAI	YACGTR	1 cut(s)	1516
BstBI	TTCGAA	1 cut(s)	1284
BstC8I	GCNNGC	1 cut(s)	684
BstDEI	CTNAG	3 cut(s)	98, 358, 806
BstDSI	CCRYGG	1 cut(s)	264
BstF5I	GGATG	2 cut(s)	755, 828
BstH2I	RGCGCY	1 cut(s)	423
BstHHI	GCGC	1 cut(s)	422
BstKTI	GATC	8 cut(s)	246, 443, 648, 1099, 1303, 1317, 1453, 1461
BstMAI	GTCTC	6 cut(s)	270, 410, 606, 626, 674, 1412
BstMBI	GATC	8 cut(s)	243, 440, 645, 1096, 1300, 1314, 1450, 1458
BstMCI	CGRYCG	1 cut(s)	246
BstMWI	GCNNNNNNNGC	6 cut(s)	692, 1010, 1019, 1022, 1028, 1200
BstNI	CCWGG	4 cut(s)	273, 618, 876, 1041
BstNSI	RCATGY	1 cut(s)	686
BstSCI	CCNGG	5 cut(s)	271, 616, 874, 1039, 1080
BstV1I	GCAGC	4 cut(s)	349, 707, 1025, 1203
BstV2I	GAAGAC	2 cut(s)	499, 1479
BstX2I	RGATCY	1 cut(s)	440
BstXI	CCANNNNNNTGG	1 cut(s)	781
BstYI	RGATCY	1 cut(s)	440
BsuRI	GGCC	4 cut(s)	726, 754, 1351, 1433
BtgI	CCRYGG	1 cut(s)	264
BtsCI	GGATG	2 cut(s)	755, 828
BtsI	GCAGTG	1 cut(s)	1416
BtsIMutI	CAGTG	3 cut(s)	51, 60, 1416
Cac8I	GCNNGC	1 cut(s)	684
CciI	TCATGA	2 cut(s)	510, 1102
CfoI	GCGC	1 cut(s)	422
Cfr10I	RCCGGY	1 cut(s)	820
Cfr13I	GGNCC	3 cut(s)	103, 196, 612
CsiI	ACCWGGT	1 cut(s)	1039
Csp6I	GTAC	3 cut(s)	980, 1279, 1354
CspAI	ACCGGT	1 cut(s)	820
CviAII	CATG	8 cut(s)	511, 592, 683, 991, 1028, 1103, 1195, 1216
CviQI	GTAC	3 cut(s)	980, 1279, 1354
DdeI	CTNAG	3 cut(s)	98, 358, 806
DinI	GGCGCC	1 cut(s)	421
DpnI	GATC	8 cut(s)	245, 442, 647, 1098, 1302, 1316, 1452, 1460
DpnII	GATC	8 cut(s)	243, 440, 645, 1096, 1300, 1314, 1450, 1458
DraI	TTTAAA	1 cut(s)	1522
EaeI	YGGCCR	1 cut(s)	752
Eam1104I	CTCTTC	1 cut(s)	318
EarI	CTCTTC	1 cut(s)	318
Ecl136II	GAGCTC	2 cut(s)	143, 805
Eco130I	CCWWGG	3 cut(s)	423, 774, 1136
Eco24I	GRGCYC	2 cut(s)	145, 807
Eco31I	GGTCTC	3 cut(s)	410, 626, 1412
Eco32I	GATATC	1 cut(s)	976
Eco47I	GGWCC	3 cut(s)	103, 196, 612
Eco53kI	GAGCTC	2 cut(s)	143, 805
Eco57I	CTGAAG	1 cut(s)	672
EcoICRI	GAGCTC	2 cut(s)	143, 805
EcoO109I	RGGNCCY	2 cut(s)	196, 612
EcoRII	CCWGG	4 cut(s)	271, 616, 874, 1039
EcoRV	GATATC	1 cut(s)	976
EcoT14I	CCWWGG	3 cut(s)	423, 774, 1136
EcoT38I	GRGCYC	2 cut(s)	145, 807
EgeI	GGCGCC	1 cut(s)	421
EheI	GGCGCC	1 cut(s)	421
ErhI	CCWWGG	3 cut(s)	423, 774, 1136
Esp3I	CGTCTC	1 cut(s)	674
FaeI	CATG	8 cut(s)	514, 595, 686, 994, 1031, 1106, 1198, 1219
FalI	AAGNNNNNCTT	2 cut(s)	416, 448
FaqI	GGGAC	1 cut(s)	625
FatI	CATG	8 cut(s)	510, 591, 682, 990, 1027, 1102, 1194, 1215
Fnu4HI	GCNGC	5 cut(s)	363, 696, 1014, 1023, 1192
FokI	GGATG	2 cut(s)	762, 815
FriOI	GRGCYC	2 cut(s)	145, 807
Fsp4HI	GCNGC	5 cut(s)	363, 696, 1014, 1023, 1192
FspBI	CTAG	2 cut(s)	71, 575
GlaI	GCGC	1 cut(s)	421
GluI	GCNGC	5 cut(s)	363, 696, 1014, 1023, 1192
GsuI	CTGGAG	2 cut(s)	129, 294
HaeII	RGCGCY	1 cut(s)	423
HaeIII	GGCC	4 cut(s)	726, 754, 1351, 1433
HapII	CCGG	4 cut(s)	643, 673, 821, 1082
HhaI	GCGC	1 cut(s)	422
Hin1I	GRCGYC	2 cut(s)	420, 667
Hin1II	CATG	8 cut(s)	514, 595, 686, 994, 1031, 1106, 1198, 1219
Hin6I	GCGC	1 cut(s)	420
HinP1I	GCGC	1 cut(s)	420
HindIII	AAGCTT	1 cut(s)	13
HinfI	GANTC	3 cut(s)	308, 334, 491
HpaII	CCGG	4 cut(s)	643, 673, 821, 1082
HphI	GGTGA	6 cut(s)	763, 841, 1009, 1055, 1117, 1270
Hpy166II	GTNNAC	2 cut(s)	355, 771
Hpy188I	TCNGA	8 cut(s)	33, 206, 253, 313, 679, 691, 809, 896
Hpy188III	TCNNGA	5 cut(s)	146, 511, 631, 643, 1103
Hpy8I	GTNNAC	2 cut(s)	355, 771
Hpy99I	CGWCG	1 cut(s)	959
HpyAV	CCTTC	5 cut(s)	187, 553, 557, 881, 1280
HpyCH4III	ACNGT	5 cut(s)	265, 500, 1112, 1168, 1183
HpyCH4IV	ACGT	3 cut(s)	667, 957, 1515
HpyCH4V	TGCA	5 cut(s)	175, 304, 365, 398, 1031
HpyF10VI	GCNNNNNNNGC	6 cut(s)	692, 1010, 1019, 1022, 1028, 1200
HpyF3I	CTNAG	3 cut(s)	98, 358, 806
HpySE526I	ACGT	3 cut(s)	667, 957, 1515
Hsp92I	GRCGYC	2 cut(s)	420, 667
Hsp92II	CATG	8 cut(s)	514, 595, 686, 994, 1031, 1106, 1198, 1219
HspAI	GCGC	1 cut(s)	420
KasI	GGCGCC	1 cut(s)	419
KflI	GGGWCCC	1 cut(s)	612
Kpn2I	TCCGGA	1 cut(s)	642
Kzo9I	GATC	8 cut(s)	243, 440, 645, 1096, 1300, 1314, 1450, 1458
LmnI	GCTCC	2 cut(s)	148, 227
Lsp1109I	GCAGC	4 cut(s)	349, 707, 1025, 1203
LweI	GCATC	2 cut(s)	780, 900
MabI	ACCWGGT	1 cut(s)	1039
MaeI	CTAG	2 cut(s)	71, 575
MaeII	ACGT	3 cut(s)	667, 957, 1515
MaeIII	GTNAC	6 cut(s)	49, 700, 1118, 1162, 1177, 1226
MalI	GATC	8 cut(s)	245, 442, 647, 1098, 1302, 1316, 1452, 1460
MboI	GATC	8 cut(s)	243, 440, 645, 1096, 1300, 1314, 1450, 1458
MboII	GAAGA	8 cut(s)	103, 335, 499, 668, 1059, 1091, 1354, 1484
MfeI	CAATTG	2 cut(s)	399, 966
MflI	RGATCY	1 cut(s)	440
MhlI	GDGCHC	2 cut(s)	145, 807
MlsI	TGGCCA	1 cut(s)	754
MluCI	AATT	8 cut(s)	373, 399, 516, 528, 935, 966, 1050, 1319
MluNI	TGGCCA	1 cut(s)	754
Mly113I	GGCGCC	1 cut(s)	420
MlyI	GAGTC	1 cut(s)	343
MmeI	TCCRAC	2 cut(s)	130, 231
Mox20I	TGGCCA	1 cut(s)	754
MroI	TCCGGA	1 cut(s)	642
MroXI	GAANNNNTTC	2 cut(s)	567, 870
MscI	TGGCCA	1 cut(s)	754
MseI	TTAA	6 cut(s)	137, 938, 1143, 1170, 1241, 1521
MslI	CAYNNNNRTG	4 cut(s)	764, 995, 1032, 1184
Msp20I	TGGCCA	1 cut(s)	754
MspA1I	CMGCKG	1 cut(s)	698
MspI	CCGG	4 cut(s)	643, 673, 821, 1082
MspR9I	CCNGG	5 cut(s)	273, 618, 876, 1041, 1082
MunI	CAATTG	2 cut(s)	399, 966
Mva1269I	GAATGC	1 cut(s)	1006
MvaI	CCWGG	4 cut(s)	273, 618, 876, 1041
MwoI	GCNNNNNNNGC	6 cut(s)	692, 1010, 1019, 1022, 1028, 1200
NarI	GGCGCC	1 cut(s)	420
NciI	CCSGG	1 cut(s)	1082
NdeII	GATC	8 cut(s)	243, 440, 645, 1096, 1300, 1314, 1450, 1458
NlaIII	CATG	8 cut(s)	514, 595, 686, 994, 1031, 1106, 1198, 1219
NlaIV	GGNNCC	7 cut(s)	58, 104, 270, 421, 442, 613, 614
NmuCI	GTSAC	4 cut(s)	49, 700, 1162, 1177
NspI	RCATGY	1 cut(s)	686
NspV	TTCGAA	1 cut(s)	1284
OliI	CACNNNNGTG	1 cut(s)	764
PaeI	GCATGC	1 cut(s)	686
PagI	TCATGA	2 cut(s)	510, 1102
PctI	GAATGC	1 cut(s)	1006
PdmI	GAANNNNTTC	2 cut(s)	567, 870
PfeI	GAWTC	2 cut(s)	308, 491
PinAI	ACCGGT	1 cut(s)	820
PkrI	GCNGC	5 cut(s)	364, 697, 1015, 1024, 1193
Ple19I	CGATCG	1 cut(s)	246
PleI	GAGTC	1 cut(s)	342
PluTI	GGCGCC	1 cut(s)	423
PpsI	GAGTC	1 cut(s)	342
Ppu21I	YACGTR	1 cut(s)	1516
PpuMI	RGGWCCY	2 cut(s)	196, 612
PshBI	ATTAAT	1 cut(s)	1143
Psp124BI	GAGCTC	2 cut(s)	145, 807
Psp5II	RGGWCCY	2 cut(s)	196, 612
Psp6I	CCWGG	4 cut(s)	271, 616, 874, 1039
PspGI	CCWGG	4 cut(s)	271, 616, 874, 1039
PspN4I	GGNNCC	7 cut(s)	58, 104, 270, 421, 442, 613, 614
PspPI	GGNCC	3 cut(s)	103, 196, 612
PspPPI	RGGWCCY	2 cut(s)	196, 612
PsuI	RGATCY	1 cut(s)	440
PvuI	CGATCG	1 cut(s)	246
PvuII	CAGCTG	1 cut(s)	698
RsaI	GTAC	3 cut(s)	981, 1280, 1355
RsaNI	GTAC	3 cut(s)	980, 1279, 1354
RseI	CAYNNNNRTG	4 cut(s)	764, 995, 1032, 1184
SacI	GAGCTC	2 cut(s)	145, 807
SaqAI	TTAA	6 cut(s)	137, 938, 1143, 1170, 1241, 1521
SatI	GCNGC	5 cut(s)	363, 696, 1014, 1023, 1192
Sau3AI	GATC	8 cut(s)	243, 440, 645, 1096, 1300, 1314, 1450, 1458
Sau96I	GGNCC	3 cut(s)	103, 196, 612
ScaI	AGTACT	1 cut(s)	1280
SchI	GAGTC	1 cut(s)	343
ScrFI	CCNGG	5 cut(s)	273, 618, 876, 1041, 1082
SduI	GDGCHC	2 cut(s)	145, 807
SexAI	ACCWGGT	1 cut(s)	1039
SfaNI	GCATC	2 cut(s)	780, 900
SfoI	GGCGCC	1 cut(s)	421
SfuI	TTCGAA	1 cut(s)	1284
SinI	GGWCC	3 cut(s)	103, 196, 612
SmiMI	CAYNNNNRTG	4 cut(s)	764, 995, 1032, 1184
SphI	GCATGC	1 cut(s)	686
Sse9I	AATT	8 cut(s)	373, 399, 516, 528, 935, 966, 1050, 1319
SsiI	CCGC	1 cut(s)	1022
SspDI	GGCGCC	1 cut(s)	419
SspMI	CTAG	2 cut(s)	71, 575
SstI	GAGCTC	2 cut(s)	145, 807
StyD4I	CCNGG	5 cut(s)	271, 616, 874, 1039, 1080
StyI	CCWWGG	3 cut(s)	423, 774, 1136
TaaI	ACNGT	5 cut(s)	265, 500, 1112, 1168, 1183
TaiI	ACGT	3 cut(s)	670, 960, 1518
TaqI	TCGA	5 cut(s)	21, 242, 378, 630, 1284
TaqII	GACCGA	1 cut(s)	650
TasI	AATT	8 cut(s)	373, 399, 516, 528, 935, 966, 1050, 1319
TatI	WGTACW	1 cut(s)	1278
TauI	GCSGC	1 cut(s)	1025
TfiI	GAWTC	2 cut(s)	308, 491
Tru1I	TTAA	6 cut(s)	137, 938, 1143, 1170, 1241, 1521
Tru9I	TTAA	6 cut(s)	137, 938, 1143, 1170, 1241, 1521
TscAI	CASTG	3 cut(s)	58, 67, 1416
TseFI	GTSAC	4 cut(s)	49, 700, 1162, 1177
TseI	GCWGC	4 cut(s)	362, 695, 1013, 1191
Tsp45I	GTSAC	4 cut(s)	49, 700, 1162, 1177
TspDTI	ATGAA	9 cut(s)	16, 251, 396, 483, 499, 1050, 1091, 1400, 1499
TspGWI	ACGGA	2 cut(s)	1214, 1406
TspRI	CASTG	3 cut(s)	58, 67, 1416
VpaK11BI	GGWCC	3 cut(s)	103, 196, 612
VspI	ATTAAT	1 cut(s)	1143
XapI	RAATTY	1 cut(s)	1319
XceI	RCATGY	1 cut(s)	686
XmnI	GAANNNNTTC	2 cut(s)	567, 870
XspI	CTAG	2 cut(s)	71, 575
ZraI	GACGTC	1 cut(s)	668
ZrmI	AGTACT	1 cut(s)	1280

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.