Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0007275

Physical Location & Seq

Genomic Coordinates Forward (+)

30064294 .. 30070395

Gene Structure 6102 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1206000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3639 bp

ATGAGGACGAAGAACAAAAACACCGACAGCGCCGGCGAGTCTCGGTTTTTAGGAACCATTTCGGCTTCTTCGCTTCGAAACCTCCTTCCCAGATCCACTTCCACCAAGAACAGCAAATCCATCTTCAACCTTCGAAACCCTAACTCCGAAAACACTCCTCCACTCAGTCCTAACATCCAGACCAATCGCCACGCTGGCGACGATCCGTCTGCTTCGCCAATCAAGCCTGTACTCTCCGATCTGTCCAAATCTCAGATCACAGAATCGGACGGCCAGGAACAAGCGTCGGCTGCTCTTAACTCACCAACGAGTGTGATTGATCGATCCAACGGTCAGGGTGAACTCCTACCACCTCTGGACCCATCAGTGAAGGTTGTGGTGAGAATTAGACCTACTAAAGATGGTGCAAATTGGGGAGATCGGACAGTTAAGAAGGTTTCTTCGCGCAGTTTGTCCGTTGGGGACCGTACATTTTCATTTGATTCGGTTTTTGATTCGAAATCCGGACAGGAAGATGTTTTTCAGATGGTTGGTGTGCCTGTGGTTGAAAATGCATTAGCCGGTTACAATACTTCCATCTTGTCATATGGACAGAGCGGAAGTGGCAAGACATACACACTATGGGGTCCGCCGAGTGCTATGGTTGAGGATGCCTCTCCTGGAAGTTGCCAAGGCATTGTTCCACGCATCTTCCAAATGTTGTTTTCTGAGATTCAAAGGGAGCAAGAGAACTCTGAAGGGAAACAGTTGAATTACCAGTTCCGTTGCTCTTTTCTCGAGATATATAACGAACAAATAGGAGATTTACTTGATCCAACCTTGCGGAACCTTGAGATTAAGGATGACCCAAAAAATGGATTTTATGTTGAGAATCTGACTGAGGAGTATGTGACTAGCTATGAGGATGTGACACAAATTTTGATTAAGGGGCTTTCAAGTAGAAAAGTTGGAGCAACTAGCATGAACTCCAAGAGCTCAAGGTCCCATATTGTTTGTACATTCATCATCGAATCCTGGTGTAAGGAGACCTCATCCAAGTGCTTTGGTAGCTCAAAAACAAGCAGGATGAGTTTTGTTGATCTTGCTGGCCTGGATAGAAATAAAGGTGATGATGATACAGGTAGAAAATGTGCGAGGGAAGACAAATATGTAAAGAAGTCATTGTCAAGGCTTGGGCTTTTGGTAAATACTCTTGCAAAAGCGCCTCAGTCTGGAAAATCTGAAGATGTTCCATTTAAGTCTTCCTGCTTAACAAATTTACTGCGAGAATCAATTGGTGGCAACTCAAAACTAACTGTCATATGTGCAATTTCCCCAGATAACAAAAATAATGGTGAGATACTGCAAACACTAAGGTTTGGAGAACGAGTGAAATCTGTTAGGAATGAACCAGTAATAAATGAAATAACAGAGGATGATGTTAATGATCTGAGTGATCAAATTCGCCAGTTAAAGGAAGAACTTATAAGAGCAAAGTCTAGTGGATCGCTTGTGATTAAGAACGGATATTTCCAAGGGAGAAATGTACGTGAAAGCTTGAATCAATTGCGGGTGAGCCTGAATCGATCTTTGATTTTACCCAGCATAGATAATGATTCTGATGAAGAGGTAAATATGGATGAACATGATATAAGGGAACTACGTGAGCAGCTAGATGAGCTGCATAATTCTTATGAGAAGGATATTAGGGACAATTCTGTCAGTAAAGATTCTGATCAGTTGGGTGGAAGTTGTGATACGGACTCGATAAGGGAAGCTGACTTTCATAGTTCAGACGAAAAAGAAATCGAGGTATTTGAAGAAATTGATCTGGAATTTTCTCAGAATGAGCTTCCTCCTAAGGACAACAACAAATTGGCAGAAAACCTTTCTCCTACCACCGCCAGTCTAGAAAACAGAAGTGGTGTTTCAATCAGTTTATGCTGTCAGTCCCCAGTCCTCCAAGACCCAACACTGTCTGAGTCTCCAAAAATTGGAAATGCACAAAGAAAGAGTGTCACTTTTGCACCAAGTTGTTCAGTTAGTCAGAACAATGTACCAGACAATTCCAAGTTCAAGTCAGATGTATTGAGACACTCACTTAACCAAAGTGAAAACATCCAATCCTCTTTGCGCTCAAGCAAGATATTTCCAGGCCCCACTGAGTCATTGGCTGCCAGCCTCCAGAGGGGCTTGCAGATAATTGACTCCCACCAGCAGAACTCCAAATCAAGCAAATCTGCAGTCTCGTTCTCCTTTGAGCATTTGGCATTGAAACCTGTTAAAGCTAATTCTTCATCTCAAACATTACCAGAAACAAGGCCATCCATAGATGGGCCATCTTCTTCGTTCCTTTGTGCATCATGTAAAAAAAGAGTGTATAAAGATGATGCCAATGAGGTCCAGGATAGCTTGAAAACATGGACTGTAGTAGTTGATGAAGCAGGAAATCCTAACCAAATGACTGAGGATGGATTGGAAGAAGCTAAAAAGGAGGAGCTTGAGAATCGTTGCAAGGAGCAAGCAGCTAAAATTGAGCAGTTAAACCAACTGGTAGAGCAATTCAAAAGTTGTGCAGAAAATGGTAAAGAAATGATACCATATGAGGAGTTCAAAGACGGAAAGAAGCTTGTGAGGGGCAGTTCTGTTGATATTCACCAACCAGAGATTATAAAGGAGAAATATGAAATACGGGAAGTTCAGACTCAGTTCGATCTTGGGTATGGGAATGCAGATTTTGATATGGATGAGAAGGAAGTACTACTTAAGGAAGTTCAGATGTTAAGGGCCAAATTGCAGTCACGCACTGATGCATTACCCAACAAGTCCACTGAGAGTCTGAGATCCTCTTTATTGTCACGATCCATGCAACTGCGAAAAAGTGGTGCATTTCAAGATAACAGCAAGGAAGAACTAGAGAAGGAAAGACAGAGATGGACAGAAATGGAGAGTGATTGGATTTCCTTAACTGATGACTTGCGGATAGATCTTGAATCCATCCGTCGACGAGCAGATAAAGTGGAGATGGAATTGAAATTGGAGAAGCAAAGCACTGAAGAGTTGGATGACGCGCTTCATAGATCTGTCCTTGGGCATGCTCGCATGGTTGAACACTATGCTGAACTACAAGATAAATACAATGACTTGGTTGCAAAGCACCGTGCAATCATGGCAGGAATAGCAGAAGTGAAGAGGGCAGCAGCAAAGGCAGGAGCAAAAGGTCGTGGATCTCGCTTTTCCAAATCCCTGGCAACAGAGCTCTCAGTATTGAGGGTGGAAAGGGAGAGGGAGAGGGAACTTTTAAAGAAGGAGAACAAGAGTCTCAAGATTCAACTGAGAGATACTGCAGAAGCTGTTCATGCTGCCGGAGAACTCCTTGTTAGGCTTAGGGAAGCTGAACATTCAGCATCAGTTGCAGAGGAGAATTTTAGGAATGTGCATCAAGAAAACGAAAAGTTGAAGAAGCAAATGGAGAAGCTTAAAAGAAAACACAAGATGGAGCTGATCACCATGAAGCAATACATGGCAGAGAGTAAACTGCCAGAGTCTGCTTTGCAACCACTAAACCGGGAGGACTCTGACATTCCGCAGAACACAATCATAGATGATGATCAGGCATGGAGAGCAGAATTCGGAGCCATATATCAAGAACATTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000278 GO:0002376 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0003676 GO:0003677 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005813 GO:0005815 GO:0005819 GO:0005829 GO:0005856 GO:0005871 GO:0005873 GO:0005875 GO:0006810 GO:0006890 GO:0006928 GO:0007017 GO:0007018 GO:0007049 GO:0008150 GO:0008283 GO:0008574 GO:0009987 GO:0015630 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019882 GO:0019884 GO:0019886 GO:0032991 GO:0042623 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0048002 GO:0048193 GO:0051179 GO:0051234 GO:0097159 GO:1901363 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

1213

Amino Acids

135.84

Weight (kDa)

5.51

Isoelectric Point (pI)

52.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	123 - 270	2e-20	Microtubule binding
Kinesin	PF00225	144 - 460	9.6e-88	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007275)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20150 AT3G20150 AT4G26660 AT5G55520 AT5G55520
fragaria_vesca	FvH4_3g23930 FvH4_3g23930 FvH4_3g23930
malus_domestica	MD03G1189700.v1.1 MD11G1206000.v1.1
prunus_persica	Prupe.4G231600_v2.0.a1
pyrus_communis	pycom03g14250 pycom03g14260 pycom11g17870
rosa_chinensis	RchiOBHm_Chr5g0042431
rosa_laevigata	RLG00000034123
rosa_multiflora	Rmu_sc0002779.1_g000010 Rmu_sc0003219.1_g000025
rosa_roxburghii	Rroxscaffold_1G00038280
rosa_rugosa	Rorug05G0200000
rosa_samantha	Rh5AG286200 Rh5BG290900 Rh5CG322900 Rh5DG300600
rosa_wichuraiana	Rw5G026560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1466, 2651
AasI	GACNNNNNNGTC	1 cut(s)	1697
AccB7I	CCANNNNNTGG	2 cut(s)	854, 1973
AccBSI	CCGCTC	1 cut(s)	597
AccI	GTMKAC	1 cut(s)	2983
AccII	CGCG	2 cut(s)	445, 3050
AccIII	TCCGGA	1 cut(s)	503
AciI	CCGC	7 cut(s)	597, 629, 823, 1549, 1881, 2959, 3566
AclWI	GGATC	8 cut(s)	87, 197, 318, 806, 1492, 2817, 2835, 3214
AcoI	YGGCCR	1 cut(s)	271
AcsI	RAATTY	6 cut(s)	915, 1255, 1440, 1814, 3403, 3608
AcuI	CTGAAG	3 cut(s)	756, 1242, 3054
AfaI	GTAC	6 cut(s)	231, 469, 997, 1527, 2037, 2739
AfiI	CCNNNNNNNGG	6 cut(s)	854, 1211, 1453, 1973, 2167, 2313
AflII	CTTAAG	1 cut(s)	2744
AhdI	GACNNNNNGTC	1 cut(s)	205
AjnI	CCWGG	7 cut(s)	273, 658, 1013, 1089, 2131, 2382, 3225
AjuI	GAANNNNNNNTTGG	2 cut(s)	663, 695
AloI	GAACNNNNNNTCC	2 cut(s)	101, 133
Alw21I	GWGCWC	2 cut(s)	977, 3240
Alw26I	GTCTC	6 cut(s)	45, 1019, 1968, 2065, 2230, 3305
AlwI	GGATC	8 cut(s)	87, 197, 318, 806, 1492, 2817, 2835, 3214
AlwNI	CAGNNNCTG	2 cut(s)	3332, 3527
Ama87I	CYCGRG	1 cut(s)	776
Aor13HI	TCCGGA	1 cut(s)	503
AoxI	GGCC	6 cut(s)	271, 1087, 2134, 2300, 2315, 2766
ApeKI	GCWGC	8 cut(s)	290, 1648, 1660, 2153, 2504, 3176, 3179, 3341
ApoI	RAATTY	6 cut(s)	915, 1255, 1440, 1814, 3403, 3608
ArsI	GACNNNNNNTTYG	4 cut(s)	901, 933, 2208, 2240
Asp700I	GAANNNNTTC	3 cut(s)	58, 1227, 3333
AspLEI	GCGC	5 cut(s)	32, 447, 1204, 2115, 3052
AspS9I	GGNCC	8 cut(s)	358, 463, 626, 981, 2135, 2315, 2380, 2766
AsuC2I	CCSGG	1 cut(s)	3548
AsuHPI	GGTGA	8 cut(s)	294, 350, 391, 1118, 1346, 1564, 2627, 3478
AsuII	TTCGAA	3 cut(s)	76, 133, 497
AvaI	CYCGRG	1 cut(s)	776
AvaII	GGWCC	5 cut(s)	358, 463, 626, 981, 2380
AxyI	CCTNAGG	1 cut(s)	1839
BanII	GRGCYC	2 cut(s)	977, 3240
BarI	GAAGNNNNNNTAC	2 cut(s)	1720, 1752
BbsI	GAAGAC	2 cut(s)	1146, 1233
Bbv12I	GWGCWC	2 cut(s)	977, 3240
BbvI	GCAGC	8 cut(s)	277, 1647, 1660, 2140, 2516, 3188, 3191, 3328
BceAI	ACGGC	1 cut(s)	286
BcgI	CGANNNNNNTGC	2 cut(s)	191, 225
BciT130I	CCWGG	7 cut(s)	275, 660, 1015, 1091, 2133, 2384, 3227
BclI	TGATCA	4 cut(s)	1435, 1714, 3483, 3589
BcnI	CCSGG	1 cut(s)	3548
BcoDI	GTCTC	6 cut(s)	45, 1019, 1968, 2065, 2230, 3305
BfaI	CTAG	6 cut(s)	894, 957, 1479, 1652, 1889, 2894
BfmI	CTRYAG	3 cut(s)	2220, 2406, 3324
BfoI	RGCGCY	2 cut(s)	33, 1205
BfrI	CTTAAG	1 cut(s)	2744
BglI	GCCNNNNNGGC	1 cut(s)	195
BglII	AGATCT	2 cut(s)	2965, 3059
BisI	GCNGC	8 cut(s)	291, 1649, 1661, 2154, 2505, 3177, 3180, 3342
BlsI	GCNGC	8 cut(s)	292, 1650, 1662, 2155, 2506, 3178, 3181, 3343
BmcAI	AGTACT	1 cut(s)	2739
Bme1390I	CCNGG	8 cut(s)	275, 660, 1015, 1091, 2133, 2384, 3227, 3548
Bme18I	GGWCC	5 cut(s)	358, 463, 626, 981, 2380
BmeRI	GACNNNNNGTC	1 cut(s)	205
BmeT110I	CYCGRG	1 cut(s)	776
BmgT120I	GGNCC	8 cut(s)	358, 463, 626, 981, 2135, 2315, 2380, 2766
BmiI	GGNNCC	8 cut(s)	55, 360, 464, 627, 827, 983, 2137, 3616
BmrFI	CCNGG	8 cut(s)	275, 660, 1015, 1091, 2133, 2384, 3227, 3548
BmrI	ACTGGG	1 cut(s)	1928
BmsI	GCATC	7 cut(s)	640, 696, 2348, 2359, 2779, 3395, 3427
BmuI	ACTGGG	1 cut(s)	1928
BpiI	GAAGAC	2 cut(s)	1146, 1233
BplI	GAGNNNNNCTC	2 cut(s)	638, 670
BpmI	CTGGAG	1 cut(s)	2147
Bpu10I	CCTNAGC	1 cut(s)	3365
Bpu14I	TTCGAA	3 cut(s)	76, 133, 497
BpuEI	CTTGAG	5 cut(s)	851, 961, 2101, 2501, 3287
BpuMI	CCSGG	1 cut(s)	3548
Bsa29I	ATCGAT	2 cut(s)	322, 1564
BsaAI	YACGTR	2 cut(s)	1529, 1643
BsaBI	GATNNNNATC	2 cut(s)	1713, 3588
BsaI	GGTCTC	1 cut(s)	1019
BsaJI	CCNNGG	4 cut(s)	670, 1513, 3067, 3225
BsaWI	WCCGGW	1 cut(s)	503
BsaXI	ACNNNNNCTCC	4 cut(s)	128, 158, 1353, 1383
Bsc4I	CCNNNNNNNGG	6 cut(s)	854, 1211, 1453, 1973, 2167, 2313
Bse118I	RCCGGY	2 cut(s)	32, 560
Bse1I	ACTGG	6 cut(s)	757, 1391, 1447, 1884, 1934, 2535
Bse21I	CCTNAGG	1 cut(s)	1839
Bse8I	GATNNNNATC	2 cut(s)	1713, 3588
BseAI	TCCGGA	1 cut(s)	503
BseBI	CCWGG	7 cut(s)	275, 660, 1015, 1091, 2133, 2384, 3227
BseCI	ATCGAT	2 cut(s)	322, 1564
BseDI	CCNNGG	4 cut(s)	670, 1513, 3067, 3225
BseJI	GATNNNNATC	2 cut(s)	1713, 3588
BseLI	CCNNNNNNNGG	6 cut(s)	854, 1211, 1453, 1973, 2167, 2313
BseNI	ACTGG	6 cut(s)	757, 1391, 1447, 1884, 1934, 2535
BseRI	GAGGAG	5 cut(s)	147, 896, 2489, 2600, 3413
BseXI	GCAGC	8 cut(s)	277, 1647, 1660, 2140, 2516, 3188, 3191, 3328
BseYI	CCCAGC	1 cut(s)	1580
BsgI	GTGCAG	1 cut(s)	2574
Bsh1236I	CGCG	2 cut(s)	445, 3050
BshFI	GGCC	6 cut(s)	273, 1089, 2136, 2302, 2317, 2768
BshVI	ATCGAT	2 cut(s)	322, 1564
BsiHKAI	GWGCWC	2 cut(s)	977, 3240
BsiHKCI	CYCGRG	1 cut(s)	776
BsiSI	CCGG	5 cut(s)	33, 504, 561, 3345, 3547
BslFI	GGGAC	4 cut(s)	476, 967, 1703, 1915
BslI	CCNNNNNNNGG	6 cut(s)	854, 1211, 1453, 1973, 2167, 2313
BsmAI	GTCTC	6 cut(s)	45, 1019, 1968, 2065, 2230, 3305
BsmFI	GGGAC	4 cut(s)	476, 967, 1703, 1915
BsmI	GAATGC	1 cut(s)	2713
BsnI	GGCC	6 cut(s)	273, 1089, 2136, 2302, 2317, 2768
Bso31I	GGTCTC	1 cut(s)	1019
BsoBI	CYCGRG	1 cut(s)	776
Bsp119I	TTCGAA	3 cut(s)	76, 133, 497
Bsp1286I	GDGCHC	2 cut(s)	977, 3240
Bsp13I	TCCGGA	1 cut(s)	503
Bsp1407I	TGTACA	1 cut(s)	995
BspACI	CCGC	7 cut(s)	597, 629, 823, 1549, 1881, 2959, 3566
BspANI	GGCC	6 cut(s)	273, 1089, 2136, 2302, 2317, 2768
BspDI	ATCGAT	2 cut(s)	322, 1564
BspEI	TCCGGA	1 cut(s)	503
BspFNI	CGCG	2 cut(s)	445, 3050
BspLI	GGNNCC	8 cut(s)	55, 360, 464, 627, 827, 983, 2137, 3616
BspMAI	CTGCAG	2 cut(s)	2224, 3328
BspPI	GGATC	8 cut(s)	87, 197, 318, 806, 1492, 2817, 2835, 3214
BspT104I	TTCGAA	3 cut(s)	76, 133, 497
BspTI	CTTAAG	1 cut(s)	2744
BspTNI	GGTCTC	1 cut(s)	1019
BsrBI	CCGCTC	1 cut(s)	597
BsrFI	RCCGGY	2 cut(s)	32, 560
BsrGI	TGTACA	1 cut(s)	995
BsrI	ACTGG	6 cut(s)	757, 1391, 1447, 1884, 1934, 2535
BssAI	RCCGGY	2 cut(s)	32, 560
BssECI	CCNNGG	4 cut(s)	670, 1513, 3067, 3225
BssT1I	CCWWGG	3 cut(s)	670, 1513, 3067
Bst2UI	CCWGG	7 cut(s)	275, 660, 1015, 1091, 2133, 2384, 3227
Bst4CI	ACNGT	8 cut(s)	332, 427, 467, 747, 1297, 1956, 2407, 3140
Bst6I	CTCTTC	3 cut(s)	1599, 3030, 3164
BstAFI	CTTAAG	1 cut(s)	2744
BstAPI	GCANNNNNTGC	1 cut(s)	3392
BstAUI	TGTACA	1 cut(s)	995
BstBAI	YACGTR	2 cut(s)	1529, 1643
BstBI	TTCGAA	3 cut(s)	76, 133, 497
BstC8I	GCNNGC	8 cut(s)	34, 196, 1087, 2158, 2174, 2502, 3075, 3079
BstFNI	CGCG	2 cut(s)	445, 3050
BstH2I	RGCGCY	2 cut(s)	33, 1205
BstHHI	GCGC	5 cut(s)	32, 447, 1204, 2115, 3052
BstMAI	GTCTC	6 cut(s)	45, 1019, 1968, 2065, 2230, 3305
BstNI	CCWGG	7 cut(s)	275, 660, 1015, 1091, 2133, 2384, 3227
BstNSI	RCATGY	1 cut(s)	3077
BstSCI	CCNGG	8 cut(s)	273, 658, 1013, 1089, 2131, 2382, 3225, 3546
BstSFI	CTRYAG	3 cut(s)	2220, 2406, 3324
BstUI	CGCG	2 cut(s)	445, 3050
BstV1I	GCAGC	8 cut(s)	277, 1647, 1660, 2140, 2516, 3188, 3191, 3328
BstV2I	GAAGAC	2 cut(s)	1146, 1233
BstX2I	RGATCY	5 cut(s)	92, 2822, 2965, 3059, 3206
BstXI	CCANNNNNNTGG	1 cut(s)	3226
BstYI	RGATCY	5 cut(s)	92, 2822, 2965, 3059, 3206
Bsu15I	ATCGAT	2 cut(s)	322, 1564
Bsu36I	CCTNAGG	1 cut(s)	1839
BsuRI	GGCC	6 cut(s)	273, 1089, 2136, 2302, 2317, 2768
BsuTUI	ATCGAT	2 cut(s)	322, 1564
BtsIMutI	CAGTG	6 cut(s)	372, 1952, 2139, 2784, 2808, 3030
Cac8I	GCNNGC	8 cut(s)	34, 196, 1087, 2158, 2174, 2502, 3075, 3079
CaiI	CAGNNNCTG	2 cut(s)	3332, 3527
CfoI	GCGC	5 cut(s)	32, 447, 1204, 2115, 3052
Cfr10I	RCCGGY	2 cut(s)	32, 560
Cfr13I	GGNCC	8 cut(s)	358, 463, 626, 981, 2135, 2315, 2380, 2766
ClaI	ATCGAT	2 cut(s)	322, 1564
CseI	GACGC	2 cut(s)	273, 3056
Csp6I	GTAC	6 cut(s)	230, 468, 996, 1526, 2036, 2738
CviQI	GTAC	6 cut(s)	230, 468, 996, 1526, 2036, 2738
DraI	TTTAAA	1 cut(s)	3282
DrdI	GACNNNNNNGTC	1 cut(s)	1697
DriI	GACNNNNNGTC	1 cut(s)	205
DseDI	GACNNNNNNGTC	1 cut(s)	1697
EaeI	YGGCCR	1 cut(s)	271
Eam1104I	CTCTTC	3 cut(s)	1599, 3030, 3164
Eam1105I	GACNNNNNGTC	1 cut(s)	205
EarI	CTCTTC	3 cut(s)	1599, 3030, 3164
EciI	GGCGGA	1 cut(s)	618
Ecl136II	GAGCTC	2 cut(s)	975, 3238
Eco130I	CCWWGG	3 cut(s)	670, 1513, 3067
Eco24I	GRGCYC	2 cut(s)	977, 3240
Eco31I	GGTCTC	1 cut(s)	1019
Eco47I	GGWCC	5 cut(s)	358, 463, 626, 981, 2380
Eco53kI	GAGCTC	2 cut(s)	975, 3238
Eco57I	CTGAAG	3 cut(s)	756, 1242, 3054
Eco81I	CCTNAGG	1 cut(s)	1839
Eco88I	CYCGRG	1 cut(s)	776
EcoICRI	GAGCTC	2 cut(s)	975, 3238
EcoO109I	RGGNCCY	2 cut(s)	981, 2135
EcoRI	GAATTC	1 cut(s)	3608
EcoRII	CCWGG	7 cut(s)	273, 658, 1013, 1089, 2131, 2382, 3225
EcoT14I	CCWWGG	3 cut(s)	670, 1513, 3067
EcoT22I	ATGCAT	2 cut(s)	556, 2794
EcoT38I	GRGCYC	2 cut(s)	977, 3240
ErhI	CCWWGG	3 cut(s)	670, 1513, 3067
FalI	AAGNNNNNCTT	2 cut(s)	2727, 2759
FaqI	GGGAC	4 cut(s)	476, 967, 1703, 1915
FauI	CCCGC	1 cut(s)	1542
FauNDI	CATATG	3 cut(s)	586, 1301, 2581
FbaI	TGATCA	4 cut(s)	1435, 1714, 3483, 3589
FblI	GTMKAC	1 cut(s)	2983
Fnu4HI	GCNGC	8 cut(s)	291, 1649, 1661, 2154, 2505, 3177, 3180, 3342
FriOI	GRGCYC	2 cut(s)	977, 3240
Fsp4HI	GCNGC	8 cut(s)	291, 1649, 1661, 2154, 2505, 3177, 3180, 3342
FspBI	CTAG	6 cut(s)	894, 957, 1479, 1652, 1889, 2894
GlaI	GCGC	5 cut(s)	31, 446, 1203, 2114, 3051
GluI	GCNGC	8 cut(s)	291, 1649, 1661, 2154, 2505, 3177, 3180, 3342
GsaI	CCCAGC	1 cut(s)	1584
GsuI	CTGGAG	1 cut(s)	2147
HaeII	RGCGCY	2 cut(s)	33, 1205
HaeIII	GGCC	6 cut(s)	273, 1089, 2136, 2302, 2317, 2768
HapII	CCGG	5 cut(s)	33, 504, 561, 3345, 3547
HgaI	GACGC	2 cut(s)	273, 3056
HhaI	GCGC	5 cut(s)	32, 447, 1204, 2115, 3052
Hin6I	GCGC	5 cut(s)	30, 445, 1202, 2113, 3050
HinP1I	GCGC	5 cut(s)	30, 445, 1202, 2113, 3050
HincII	GTYRAC	1 cut(s)	2984
HindII	GTYRAC	1 cut(s)	2984
HindIII	AAGCTT	3 cut(s)	1534, 2606, 3455
HpaII	CCGG	5 cut(s)	33, 504, 561, 3345, 3547
HphI	GGTGA	8 cut(s)	294, 350, 391, 1118, 1346, 1564, 2627, 3478
Hpy166II	GTNNAC	4 cut(s)	341, 2808, 2984, 3515
Hpy8I	GTNNAC	4 cut(s)	341, 2808, 2984, 3515
Hpy99I	CGWCG	4 cut(s)	203, 289, 2985, 2988
HpyAV	CCTTC	9 cut(s)	95, 140, 364, 427, 731, 1672, 2725, 2893, 3280
HpyCH4III	ACNGT	8 cut(s)	332, 427, 467, 747, 1297, 1956, 2407, 3140
HpyCH4IV	ACGT	2 cut(s)	1528, 1642
HpySE526I	ACGT	2 cut(s)	1528, 1642
HspAI	GCGC	5 cut(s)	30, 445, 1202, 2113, 3050
Kpn2I	TCCGGA	1 cut(s)	503
KroI	GCCGGC	1 cut(s)	32
KroNI	GCCGGC	1 cut(s)	34
Ksp22I	TGATCA	4 cut(s)	1435, 1714, 3483, 3589
LmnI	GCTCC	7 cut(s)	721, 950, 2476, 2497, 3191, 3478, 3614
Lsp1109I	GCAGC	8 cut(s)	277, 1647, 1660, 2140, 2516, 3188, 3191, 3328
LweI	GCATC	7 cut(s)	640, 696, 2348, 2359, 2779, 3395, 3427
MaeI	CTAG	6 cut(s)	894, 957, 1479, 1652, 1889, 2894
MaeII	ACGT	2 cut(s)	1528, 1642
MaeIII	GTNAC	6 cut(s)	563, 889, 907, 1996, 2778, 2835
MbiI	CCGCTC	1 cut(s)	597
MfeI	CAATTG	2 cut(s)	1272, 1544
MflI	RGATCY	5 cut(s)	92, 2822, 2965, 3059, 3206
MhlI	GDGCHC	2 cut(s)	977, 3240
MmeI	TCCRAC	4 cut(s)	351, 839, 928, 3021
Mph1103I	ATGCAT	2 cut(s)	556, 2794
MreI	CGCCGGCG	1 cut(s)	32
MroI	TCCGGA	1 cut(s)	503
MroNI	GCCGGC	1 cut(s)	32
MroXI	GAANNNNTTC	3 cut(s)	58, 1227, 3333
MslI	CAYNNNNRTG	1 cut(s)	1036
MspCI	CTTAAG	1 cut(s)	2744
MspI	CCGG	5 cut(s)	33, 504, 561, 3345, 3547
MspR9I	CCNGG	8 cut(s)	275, 660, 1015, 1091, 2133, 2384, 3227, 3548
MunI	CAATTG	2 cut(s)	1272, 1544
Mva1269I	GAATGC	1 cut(s)	2713
MvaI	CCWGG	7 cut(s)	275, 660, 1015, 1091, 2133, 2384, 3227
MvnI	CGCG	2 cut(s)	445, 3050
NaeI	GCCGGC	1 cut(s)	34
NciI	CCSGG	1 cut(s)	3548
NdeI	CATATG	3 cut(s)	586, 1301, 2581
NgoMIV	GCCGGC	1 cut(s)	32
NlaIV	GGNNCC	8 cut(s)	55, 360, 464, 627, 827, 983, 2137, 3616
NmeAIII	GCCGAG	1 cut(s)	657
NmuCI	GTSAC	5 cut(s)	889, 907, 1996, 2778, 2835
NsiI	ATGCAT	2 cut(s)	556, 2794
NspI	RCATGY	1 cut(s)	3077
NspV	TTCGAA	3 cut(s)	76, 133, 497
PaeI	GCATGC	1 cut(s)	3077
PaeR7I	CTCGAG	1 cut(s)	776
PcsI	WCGNNNNNNNCGW	1 cut(s)	198
PctI	GAATGC	1 cut(s)	2713
PdiI	GCCGGC	1 cut(s)	34
PdmI	GAANNNNTTC	3 cut(s)	58, 1227, 3333
PflMI	CCANNNNNTGG	2 cut(s)	854, 1973
PfoI	TCCNGGA	2 cut(s)	658, 2382
PkrI	GCNGC	8 cut(s)	292, 1650, 1662, 2155, 2506, 3178, 3181, 3343
Ppu21I	YACGTR	2 cut(s)	1529, 1643
PpuMI	RGGWCCY	1 cut(s)	981
PsiI	TTATAA	2 cut(s)	1466, 2651
Psp124BI	GAGCTC	2 cut(s)	977, 3240
Psp5II	RGGWCCY	1 cut(s)	981
Psp6I	CCWGG	7 cut(s)	273, 658, 1013, 1089, 2131, 2382, 3225
PspFI	CCCAGC	1 cut(s)	1580
PspGI	CCWGG	7 cut(s)	273, 658, 1013, 1089, 2131, 2382, 3225
PspN4I	GGNNCC	8 cut(s)	55, 360, 464, 627, 827, 983, 2137, 3616
PspPI	GGNCC	8 cut(s)	358, 463, 626, 981, 2135, 2315, 2380, 2766
PspPPI	RGGWCCY	1 cut(s)	981
PstI	CTGCAG	2 cut(s)	2224, 3328
PstNI	CAGNNNCTG	2 cut(s)	3332, 3527
PsuI	RGATCY	5 cut(s)	92, 2822, 2965, 3059, 3206
RsaI	GTAC	6 cut(s)	231, 469, 997, 1527, 2037, 2739
RsaNI	GTAC	6 cut(s)	230, 468, 996, 1526, 2036, 2738
RseI	CAYNNNNRTG	1 cut(s)	1036
SacI	GAGCTC	2 cut(s)	977, 3240
SalI	GTCGAC	1 cut(s)	2982
SatI	GCNGC	8 cut(s)	291, 1649, 1661, 2154, 2505, 3177, 3180, 3342
Sau96I	GGNCC	8 cut(s)	358, 463, 626, 981, 2135, 2315, 2380, 2766
ScaI	AGTACT	1 cut(s)	2739
ScrFI	CCNGG	8 cut(s)	275, 660, 1015, 1091, 2133, 2384, 3227, 3548
SduI	GDGCHC	2 cut(s)	977, 3240
SfaNI	GCATC	7 cut(s)	640, 696, 2348, 2359, 2779, 3395, 3427
SfcI	CTRYAG	3 cut(s)	2220, 2406, 3324
Sfr274I	CTCGAG	1 cut(s)	776
SfuI	TTCGAA	3 cut(s)	76, 133, 497
SgrAI	CRCCGGYG	1 cut(s)	32
SgrDI	CGTCGACG	1 cut(s)	2982
SinI	GGWCC	5 cut(s)	358, 463, 626, 981, 2380
SlaI	CTCGAG	1 cut(s)	776
SmiMI	CAYNNNNRTG	1 cut(s)	1036
SmlI	CTYRAG	7 cut(s)	776, 830, 976, 2116, 2480, 2744, 3302
SmoI	CTYRAG	7 cut(s)	776, 830, 976, 2116, 2480, 2744, 3302
SphI	GCATGC	1 cut(s)	3077
SsiI	CCGC	7 cut(s)	597, 629, 823, 1549, 1881, 2959, 3566
SspMI	CTAG	6 cut(s)	894, 957, 1479, 1652, 1889, 2894
SstI	GAGCTC	2 cut(s)	977, 3240
StyD4I	CCNGG	8 cut(s)	273, 658, 1013, 1089, 2131, 2382, 3225, 3546
StyI	CCWWGG	3 cut(s)	670, 1513, 3067
TaaI	ACNGT	8 cut(s)	332, 427, 467, 747, 1297, 1956, 2407, 3140
TaiI	ACGT	2 cut(s)	1531, 1645
TatI	WGTACW	3 cut(s)	229, 995, 2737
TscAI	CASTG	6 cut(s)	372, 1959, 2146, 2791, 2815, 3037
TseFI	GTSAC	5 cut(s)	889, 907, 1996, 2778, 2835
TseI	GCWGC	8 cut(s)	290, 1648, 1660, 2153, 2504, 3176, 3179, 3341
Tsp45I	GTSAC	5 cut(s)	889, 907, 1996, 2778, 2835
TspGWI	ACGGA	7 cut(s)	195, 445, 752, 1518, 1754, 2613, 2969
TspRI	CASTG	6 cut(s)	372, 1959, 2146, 2791, 2815, 3037
Van91I	CCANNNNNTGG	2 cut(s)	854, 1973
Vha464I	CTTAAG	1 cut(s)	2744
VpaK11BI	GGWCC	5 cut(s)	358, 463, 626, 981, 2380
XapI	RAATTY	6 cut(s)	915, 1255, 1440, 1814, 3403, 3608
XbaI	TCTAGA	1 cut(s)	1888
XceI	RCATGY	1 cut(s)	3077
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2146
XhoI	CTCGAG	1 cut(s)	776
XmiI	GTMKAC	1 cut(s)	2983
XmnI	GAANNNNTTC	3 cut(s)	58, 1227, 3333
XspI	CTAG	6 cut(s)	894, 957, 1479, 1652, 1889, 2894
ZrmI	AGTACT	1 cut(s)	2739
Zsp2I	ATGCAT	2 cut(s)	556, 2794

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.