MD11G1251600.v1.1

3-hydroxyacyl-CoA dehydrogenase, C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0015035
Physical Location & Seq
Reverse (-)
36360595 .. 36363589
2995 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1251600.v1.1.491

Sequence Viewer

Length: 900 bp
ATGGCGGAGATCAAGAAATTTAAGACAATCGGAGTGGTGGGCAGCGGCCAAATGGGCTCAGGAATAGCCCAACTAGCCGCCACGTATGGCTTCGACGTTTGGCTCCTTGACACCGACCCGGAGGCTCTCTCAAGAGCCACCAAGTCCATCTCTAGCTCCATCCAGCGCCTAGTTTCCAAAGGGCAGCTCAGCCAGGATGTAAGAAAAGATGCTTTAGAGCGTCTACAATATACGTCAAATGTTGAAAATTTTTGTTCGGCAGATATTATTATTGAAGCTATTGTGGAATCTGAAGATGTGAAGAAAAAGTTATTTCTTCAACTAGATAAGATTGCAAAACGTTCGGCCATACTGGCTTCTAATACAAGTTCCATCTCCATTACTCGTCTTGCATCAGCAACTAGCAGACCACACCAGGTGATTGGCATGCATTTTATGAATCCTCCTCCTATAATGAAGCTTGTTGAGATTGTGCGAGGAGCAGACACATCAGATGAGACATTTGATATCACAAAAGCCTTGGCAGAGAGGTTTGGCAAGACCGTAATATGCTCTCGAGATTATGCCGGGTTTGTTGTGAACAGGATCCTAATGCCGATGATAAATGAAGCATTTTATGCACTTTACACAGGTGTGGCGACCAAAGAAGACATTGATGCAGGAATGAAGCTGGGAACAAATCATCCAATGGGTCCTCTTGAGCTTGCAGATTTTATTGGATTGGATATCTGCTTGTCTATTCTGAGAGTTCTTCATGCTGGCCTTGGGGACAATAAATACGCTCCGTGCCCCATTCTTGTACAGTACGTTGATGCAGGTCGCCTTGGAAGAAAACGAGGTATTGGGGTATACGACTACCGTCAAACGCCTGAATCAGTAAAACCTTCATCTAAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00360 ko00362 ko00650 ko01100 ko01120 map00360 map00362 map00650 map01100 map01120
Pfam Domains
Protein Families
3HCDH 3HCDH_N

Protein Analysis

300

Amino Acids

32.69

Weight (kDa)

8.55

Isoelectric Point (pI)

33.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
3HCDH_N PF02737 9 - 187 2.8e-66 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain
3HCDH PF00725 190 - 286 1.5e-35 3-hydroxyacyl-CoA dehydrogenase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015035)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15290 AT3G15290
fragaria_vesca FvH4_3g19690
malus_domestica MD11G1251600.v1.1
prunus_persica Prupe.4G177500_v2.0.a1 Prupe.4G177500_v2.0.a1
pyrus_communis pycom11g22290
rosa_chinensis RchiOBHm_Chr5g0032991
rosa_laevigata RLG00000033441
rosa_multiflora Rmu_sc0000986.1_g000019
rosa_roxburghii Rroxscaffold_1G00047050
rosa_rugosa Rorug05G0134700
rosa_samantha Rh5AG227600 Rh5BG229100 Rh5DG233800
rosa_wichuraiana Rw5G020840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 806
AccI GTMKAC 2 cut(s) 223, 849
AciI CCGC 3 cut(s) 5, 45, 78
AclI AACGTT 1 cut(s) 340
AclWI GGATC 2 cut(s) 580, 593
AcoI YGGCCR 2 cut(s) 46, 345
AcsI RAATTY 2 cut(s) 17, 247
AcuI CTGAAG 1 cut(s) 312
AdeI CACNNNGTG 1 cut(s) 418
AfaI GTAC 2 cut(s) 801, 806
AgsI TTSAA 3 cut(s) 245, 275, 320
AjnI CCWGG 2 cut(s) 192, 414
AleI CACNNNNGTG 1 cut(s) 632
AloI GAACNNNNNNTCC 2 cut(s) 667, 699
AluBI AGCT 6 cut(s) 156, 187, 278, 460, 670, 703
AluI AGCT 6 cut(s) 156, 187, 278, 460, 670, 703
Alw26I GTCTC 1 cut(s) 491
AlwI GGATC 2 cut(s) 580, 593
Ama87I CYCGRG 1 cut(s) 555
AoxI GGCC 3 cut(s) 46, 345, 760
ApeKI GCWGC 2 cut(s) 42, 184
ApoI RAATTY 2 cut(s) 17, 247
AspLEI GCGC 1 cut(s) 168
AspS9I GGNCC 1 cut(s) 692
AsuC2I CCSGG 2 cut(s) 119, 568
AsuHPI GGTGA 1 cut(s) 430
AvaI CYCGRG 1 cut(s) 555
AvaII GGWCC 1 cut(s) 692
BaeGI GKGCMC 1 cut(s) 791
BamHI GGATCC 1 cut(s) 585
BanII GRGCYC 1 cut(s) 59
BbsI GAAGAC 1 cut(s) 654
BbvI GCAGC 2 cut(s) 54, 196
BccI CCATC 3 cut(s) 155, 167, 380
BcgI CGANNNNNNTGC 2 cut(s) 324, 358
BciT130I CCWGG 2 cut(s) 194, 416
BcnI CCSGG 2 cut(s) 119, 568
BcoDI GTCTC 1 cut(s) 491
BfaI CTAG 5 cut(s) 74, 153, 170, 323, 402
BfoI RGCGCY 1 cut(s) 169
BfuAI ACCTGC 1 cut(s) 806
BglI GCCNNNNNGGC 2 cut(s) 54, 353
BisI GCNGC 4 cut(s) 43, 46, 78, 185
BlpI GCTNAGC 1 cut(s) 188
BlsI GCNGC 4 cut(s) 44, 47, 79, 186
Bme1390I CCNGG 4 cut(s) 119, 194, 416, 568
Bme18I GGWCC 1 cut(s) 692
BmeT110I CYCGRG 1 cut(s) 555
BmgT120I GGNCC 1 cut(s) 692
BmiI GGNNCC 3 cut(s) 104, 587, 693
BmrFI CCNGG 4 cut(s) 119, 194, 416, 568
BmsI GCATC 4 cut(s) 199, 401, 646, 802
BoxI GACNNNNGTC 1 cut(s) 858
BpiI GAAGAC 1 cut(s) 654
BplI GAGNNNNNCTC 2 cut(s) 113, 145
Bpu10I CCTNAGC 1 cut(s) 58
Bpu1102I GCTNAGC 1 cut(s) 188
BpuEI CTTGAG 2 cut(s) 115, 719
BpuMI CCSGG 2 cut(s) 119, 568
BsaAI YACGTR 1 cut(s) 84
BsaJI CCNNGG 3 cut(s) 519, 763, 823
Bse1I ACTGG 1 cut(s) 357
BseBI CCWGG 2 cut(s) 194, 416
BseDI CCNNGG 3 cut(s) 519, 763, 823
BseGI GGATG 3 cut(s) 159, 202, 682
BseMII CTCAG 3 cut(s) 72, 202, 734
BseNI ACTGG 1 cut(s) 357
BseRI GAGGAG 2 cut(s) 435, 492
BseSI GKGCMC 1 cut(s) 791
BseXI GCAGC 2 cut(s) 54, 196
BseYI CCCAGC 1 cut(s) 670
BshFI GGCC 3 cut(s) 48, 347, 762
BsiHKCI CYCGRG 1 cut(s) 555
BsiSI CCGG 2 cut(s) 119, 567
BslFI GGGAC 1 cut(s) 782
BsmAI GTCTC 1 cut(s) 491
BsmFI GGGAC 1 cut(s) 782
BsnI GGCC 3 cut(s) 48, 347, 762
BsoBI CYCGRG 1 cut(s) 555
Bsp1286I GDGCHC 2 cut(s) 59, 791
Bsp1407I TGTACA 1 cut(s) 799
Bsp143I GATC 2 cut(s) 9, 585
Bsp1720I GCTNAGC 1 cut(s) 188
BspACI CCGC 3 cut(s) 5, 45, 78
BspANI GGCC 3 cut(s) 48, 347, 762
BspCNI CTCAG 3 cut(s) 71, 201, 735
BspLI GGNNCC 3 cut(s) 104, 587, 693
BspMI ACCTGC 1 cut(s) 806
BspPI GGATC 2 cut(s) 580, 593
BsrGI TGTACA 1 cut(s) 799
BsrI ACTGG 1 cut(s) 357
BssECI CCNNGG 3 cut(s) 519, 763, 823
BssMI GATC 2 cut(s) 9, 585
BssNAI GTATAC 1 cut(s) 850
BssT1I CCWWGG 3 cut(s) 519, 763, 823
Bst1107I GTATAC 1 cut(s) 850
Bst2UI CCWGG 2 cut(s) 194, 416
Bst4CI ACNGT 3 cut(s) 544, 804, 860
BstAPI GCANNNNNTGC 1 cut(s) 617
BstAUI TGTACA 1 cut(s) 799
BstBAI YACGTR 1 cut(s) 84
BstC8I GCNNGC 3 cut(s) 428, 705, 760
BstDEI CTNAG 3 cut(s) 58, 188, 743
BstF5I GGATG 3 cut(s) 159, 202, 682
BstH2I RGCGCY 1 cut(s) 169
BstHHI GCGC 1 cut(s) 168
BstKTI GATC 2 cut(s) 12, 588
BstMAI GTCTC 1 cut(s) 491
BstMBI GATC 2 cut(s) 9, 585
BstMWI GCNNNNNNNGC 4 cut(s) 54, 74, 353, 617
BstNI CCWGG 2 cut(s) 194, 416
BstNSI RCATGY 1 cut(s) 430
BstPAI GACNNNNGTC 1 cut(s) 858
BstSCI CCNGG 4 cut(s) 117, 192, 414, 566
BstSLI GKGCMC 1 cut(s) 791
BstV1I GCAGC 2 cut(s) 54, 196
BstV2I GAAGAC 1 cut(s) 654
BstX2I RGATCY 1 cut(s) 585
BstXI CCANNNNNNTGG 1 cut(s) 422
BstYI RGATCY 1 cut(s) 585
BstZ17I GTATAC 1 cut(s) 850
BsuRI GGCC 3 cut(s) 48, 347, 762
BtsCI GGATG 3 cut(s) 159, 202, 682
BveI ACCTGC 1 cut(s) 806
Cac8I GCNNGC 3 cut(s) 428, 705, 760
CfoI GCGC 1 cut(s) 168
Cfr13I GGNCC 1 cut(s) 692
CseI GACGC 1 cut(s) 209
CsiI ACCWGGT 1 cut(s) 414
Csp6I GTAC 2 cut(s) 800, 805
CspCI CAANNNNNGTGG 2 cut(s) 15, 50
CviAII CATG 2 cut(s) 427, 755
CviQI GTAC 2 cut(s) 800, 805
DdeI CTNAG 3 cut(s) 58, 188, 743
DpnI GATC 2 cut(s) 11, 587
DpnII GATC 2 cut(s) 9, 585
DraIII CACNNNGTG 1 cut(s) 418
EaeI YGGCCR 2 cut(s) 46, 345
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 519, 763, 823
Eco24I GRGCYC 1 cut(s) 59
Eco32I GATATC 2 cut(s) 508, 727
Eco47I GGWCC 1 cut(s) 692
Eco57I CTGAAG 1 cut(s) 312
Eco88I CYCGRG 1 cut(s) 555
EcoO109I RGGNCCY 1 cut(s) 692
EcoRII CCWGG 2 cut(s) 192, 414
EcoRV GATATC 2 cut(s) 508, 727
EcoT14I CCWWGG 3 cut(s) 519, 763, 823
EcoT22I ATGCAT 1 cut(s) 432
EcoT38I GRGCYC 1 cut(s) 59
ErhI CCWWGG 3 cut(s) 519, 763, 823
FaeI CATG 2 cut(s) 430, 758
FaqI GGGAC 1 cut(s) 782
FatI CATG 2 cut(s) 426, 754
FblI GTMKAC 2 cut(s) 223, 849
Fnu4HI GCNGC 4 cut(s) 43, 46, 78, 185
FokI GGATG 3 cut(s) 146, 209, 669
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 4 cut(s) 43, 46, 78, 185
FspBI CTAG 5 cut(s) 74, 153, 170, 323, 402
GlaI GCGC 1 cut(s) 167
GluI GCNGC 4 cut(s) 43, 46, 78, 185
GsaI CCCAGC 1 cut(s) 674
HaeII RGCGCY 1 cut(s) 169
HaeIII GGCC 3 cut(s) 48, 347, 762
HapII CCGG 2 cut(s) 119, 567
HgaI GACGC 1 cut(s) 209
HhaI GCGC 1 cut(s) 168
Hin1II CATG 2 cut(s) 430, 758
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HindIII AAGCTT 1 cut(s) 458
HinfI GANTC 3 cut(s) 287, 439, 872
HpaII CCGG 2 cut(s) 119, 567
HphI GGTGA 1 cut(s) 430
Hpy166II GTNNAC 3 cut(s) 224, 580, 850
Hpy188I TCNGA 4 cut(s) 32, 292, 493, 744
Hpy188III TCNNGA 6 cut(s) 13, 60, 132, 555, 557, 698
Hpy8I GTNNAC 3 cut(s) 224, 580, 850
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 1 cut(s) 894
HpyCH4III ACNGT 3 cut(s) 544, 804, 860
HpyCH4IV ACGT 5 cut(s) 83, 96, 233, 340, 807
HpyCH4V TGCA 7 cut(s) 335, 392, 430, 620, 659, 707, 815
HpyF10VI GCNNNNNNNGC 4 cut(s) 54, 74, 353, 617
HpyF3I CTNAG 3 cut(s) 58, 188, 743
HpySE526I ACGT 5 cut(s) 83, 96, 233, 340, 807
Hsp92II CATG 2 cut(s) 430, 758
HspAI GCGC 1 cut(s) 166
Kzo9I GATC 2 cut(s) 9, 585
LmnI GCTCC 4 cut(s) 108, 161, 479, 787
Lsp1109I GCAGC 2 cut(s) 54, 196
LweI GCATC 4 cut(s) 199, 401, 646, 802
MabI ACCWGGT 1 cut(s) 414
MaeI CTAG 5 cut(s) 74, 153, 170, 323, 402
MaeII ACGT 5 cut(s) 83, 96, 233, 340, 807
MalI GATC 2 cut(s) 11, 587
MboI GATC 2 cut(s) 9, 585
MboII GAAGA 6 cut(s) 305, 308, 313, 659, 743, 840
MflI RGATCY 1 cut(s) 585
MhlI GDGCHC 2 cut(s) 59, 791
MluCI AATT 2 cut(s) 17, 247
MnlI CCTC 7 cut(s) 115, 453, 456, 470, 522, 705, 830
Mph1103I ATGCAT 1 cut(s) 432
MseI TTAA 1 cut(s) 21
MslI CAYNNNNRTG 1 cut(s) 632
MspA1I CMGCKG 1 cut(s) 45
MspI CCGG 2 cut(s) 119, 567
MspR9I CCNGG 4 cut(s) 119, 194, 416, 568
MvaI CCWGG 2 cut(s) 194, 416
MwoI GCNNNNNNNGC 4 cut(s) 54, 74, 353, 617
NciI CCSGG 2 cut(s) 119, 568
NdeII GATC 2 cut(s) 9, 585
NlaIII CATG 2 cut(s) 430, 758
NlaIV GGNNCC 3 cut(s) 104, 587, 693
NsiI ATGCAT 1 cut(s) 432
NspI RCATGY 1 cut(s) 430
OliI CACNNNNGTG 1 cut(s) 632
PaeI GCATGC 1 cut(s) 430
PaeR7I CTCGAG 1 cut(s) 555
PfeI GAWTC 3 cut(s) 287, 439, 872
PkrI GCNGC 4 cut(s) 44, 47, 79, 186
Ppu21I YACGTR 1 cut(s) 84
PpuMI RGGWCCY 1 cut(s) 692
PshAI GACNNNNGTC 1 cut(s) 858
Psp1406I AACGTT 1 cut(s) 340
Psp5II RGGWCCY 1 cut(s) 692
Psp6I CCWGG 2 cut(s) 192, 414
PspFI CCCAGC 1 cut(s) 670
PspGI CCWGG 2 cut(s) 192, 414
PspN4I GGNNCC 3 cut(s) 104, 587, 693
PspPI GGNCC 1 cut(s) 692
PspPPI RGGWCCY 1 cut(s) 692
PsuI RGATCY 1 cut(s) 585
RsaI GTAC 2 cut(s) 801, 806
RsaNI GTAC 2 cut(s) 800, 805
RseI CAYNNNNRTG 1 cut(s) 632
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 4 cut(s) 43, 46, 78, 185
Sau3AI GATC 2 cut(s) 9, 585
Sau96I GGNCC 1 cut(s) 692
ScrFI CCNGG 4 cut(s) 119, 194, 416, 568
SduI GDGCHC 2 cut(s) 59, 791
SexAI ACCWGGT 1 cut(s) 414
SfaNI GCATC 4 cut(s) 199, 401, 646, 802
Sfr274I CTCGAG 1 cut(s) 555
SinI GGWCC 1 cut(s) 692
SlaI CTCGAG 1 cut(s) 555
SmiMI CAYNNNNRTG 1 cut(s) 632
SmlI CTYRAG 3 cut(s) 130, 555, 698
SmoI CTYRAG 3 cut(s) 130, 555, 698
SphI GCATGC 1 cut(s) 430
Sse9I AATT 2 cut(s) 17, 247
SsiI CCGC 3 cut(s) 5, 45, 78
SspMI CTAG 5 cut(s) 74, 153, 170, 323, 402
StyD4I CCNGG 4 cut(s) 117, 192, 414, 566
StyI CCWWGG 3 cut(s) 519, 763, 823
TaaI ACNGT 3 cut(s) 544, 804, 860
TaiI ACGT 5 cut(s) 86, 99, 236, 343, 810
TaqI TCGA 2 cut(s) 93, 556
TasI AATT 2 cut(s) 17, 247
TatI WGTACW 1 cut(s) 799
TauI GCSGC 2 cut(s) 48, 80
TfiI GAWTC 3 cut(s) 287, 439, 872
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TseI GCWGC 2 cut(s) 42, 184
TspDTI ATGAA 6 cut(s) 452, 470, 621, 680, 743, 876
TspGWI ACGGA 1 cut(s) 774
VpaK11BI GGWCC 1 cut(s) 692
XapI RAATTY 2 cut(s) 17, 247
XceI RCATGY 1 cut(s) 430
XhoI CTCGAG 1 cut(s) 555
XmiI GTMKAC 2 cut(s) 223, 849
XspI CTAG 5 cut(s) 74, 153, 170, 323, 402
Zsp2I ATGCAT 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.