Rorug05G0134700

3-hydroxyacyl-CoA dehydrogenase, C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0015035
Physical Location & Seq
Forward (+)
12077876 .. 12080644
2769 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0134700.1

Sequence Viewer

Length: 2769 bp
ATGGCTACTATGGCCTTATCTGCAACGGACCTCTTAATTGGAAAACTTCTGACAATTCTTGAGAACGAAGGAAAAACCATAGCCGATGTTCGTGATGAAGTTGAAAAGATTAAGGAGGAGTTTCTAAGCATGAAAGCTTTCCTAGTGGATGCGGAGACCAACAAAACAAAAACTGAAGGAGGGAAATTGTGGGTTGCAAGCATCAGAGACTTAGCCAATCAAGTTGAAGATATCATTGATGAATTCATGTATCACATGTATGAGAAGCAAAGTGGGGGTCGACTTTCAAGGGGGCTGCACAAAACCATTGGCGCTCCAAGTCATCTTTGGTTCAGGCATAAAATTGCAAAGAAGTTGAAGAAAATTACTGAAATGATCAAAGCCATTCCAGAGAGGAATCAAAGGTACGGTGTTGGCCATGTAGGAGGAGCAACTACATCTGAAGATATTCACAAATTGGTGCAGAACCAAGTGGAGTCCTCTATTTTCATTATGGAAGACGAACTGGTTGGGATTGAAGGCAAGAAGCAAATACTGATGGGATGGCTCATGGATGAAGAGCAACACCAAACTGTTATATCTGTGGTTGGCATGGGAGGCTCTGGAAAGACAACTCTAGTTGCCAAGACCTTCAACAATGAAAAGGTGAAGAGACATTTCAATTGTTACGCATGGATTACCATTTCCCAAACTTATGATGTCGTACACTTGTTTCGAAGCTTGATTAGGGAACTCTACAAATCAAGGAAGGAAGAGTACTCTGGAAATTTGAATGCCATGAGCCGCATAGAATTGCTAGAGTTACTAGTTAACTACTTGGAGTCCAAAAGATACCTGGTTGTTCTAGATGACGTGTGGGATATAAAAGTTTGGAGAGAAATAAAGGTATCACTTCAAGATAGGCAACTCGGAAGTCGAATCATAGTTACAACTCGAAAAGAAGATGTAGCATGCAATTCTTTTGGAGTTAAAAGCCATGTTCACCATATTCAACCCCTGCTAAAGAATGAGACTTGGGAGCTCTTTAGCAAGAAAGCATTCTCAACTAATCAGGATAAAACTTGTCCTACTGAGCTTGAATCATTAGCTTGCCAACTTGTGGATAAGTGTGAAGGCCTTCCTTTGGCTATTGTGTCTTTAGGTGGTCTTATGTCTTCTAAGAAGTCACTGGATCAATGGGACCAAGTCCTCAACAGCTTTAATTGGCATCTACATAAAAATTCATTGCTAGACCCTATGAAAAACATCTTGTTCATGAGTTTCAGTGATTTGCCATCTCAATTGAAACATTGCTTCCTCTATTGCTCTCTATTCCCAGAAGATTTTGTAATTAAAAGAAAAAGGCTCACTAGATTGTGGATAGCCGAAGGATTTGCTGAACTTGTGAAAGGGGTCACACCAGAAGAAGTTGCAGACGACAATCTTATGGAGCTCTGTTTCCGTAGCATGTTACAAGTTGTAAGGAGAAATGGTACAGGAAGGCCGAAACAAGTTAAGATGCATGATCTGATGCGGGAGCTTGCTTTGTCGACAGCCGAGAAGGAAAAATTTGGTCTTGTCTATGATGGGAAAGAAGTAATGGAAGAGATCTCAACCCGTCGCTTGTCAATTCACACAACGAGTGATGGAGGAGAAATTAAATCATTGCCGGGTAAGTCAAAGATTCGTTCTCTTCTTGTCTTTGCAACTGACATGTCCTCATCCTCTTTCTCAAATACACTGGTTTCTCGATTCAAATTGTTGAGGACTCTAGACTTGGAGGATGTCCAGATTGATAAACTACCAGATGCCGTTGTTTACTTGTTCAACTTGAGATATTTAAATTTGAGGAACACTTTAATCAAGAAACTTCCCGAGTCCATAGGGCGTCTCCGCAACCTTCAATTTCTGAACATCAAGGATTCTAAGATAGCGTCGCTTCCACAAGGAAGTGCAAAGTTGCTGAACCTGCGCCATCTAATTATGTATCGCTACACTGGAAGCTTTCTAGACTTTAATTATGTGAGTGGGATAAAAGTGCCATCAAATATTTGTCAGTTGCAGAAATTGCAAAGTTTATCAATGATTGAATCAGAAGGGGATACTATTAGATTTATCGGAAACATGACTCAACTTAAGAGCATCGGTATATCAAATGTGAAAGAAAGAGATGAGATTGACCTTTGGGTCTCAATTGAAAAGTTGAATCTGCTTCAATCTATGGTTTTAATGGCAAGTAATGAAGAGGAAATCCTTCCAGTGAAAGCACAATGTCCACCTCTTTCGCGCCTTCGAACGCTTTATTTGACTGGCAGACTGCAAAACGCATTACCTTGGTTTTCCTCACTGCAGAGCCTTGTACATCTGTCTCTGCGTTGGTCTAGACTTGAAGAGGATTTACTACCTCAAATTGAAGCACTGGCCAATCTGACATGGCTTTATCTTTGTAATGCATATGTTGGGGAAGAGTTGTGTTTTCGCAGAGGCTTTGTAAAGCTGGAGAGGTTGCATTTGTTGAATTTTACCTCAGTGAAAAAGATAAGTATAGAAAGAGGGGTGATGCCAAATCTCCGGTACTTGTGGGTTGACAACTTCATGGAGTTGAAGACAGTGCCACTAGGCATTGAATACCTTTCTAATCTACAAACTTTGGGCCTGGCAATTGTTCCCATTGAAATTATACAGCCCATTCGGATAGGAGGTGTGGATCGGTCTAAGGTACAACACATCTCGGATATCAGACATATTTTCCAACAATCATCCAATCGGATCCACGAAAACTTGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00360 ko00362 ko00650 ko01100 ko01120 map00360 map00362 map00650 map01100 map01120
Pfam Domains
Protein Families
3HCDH 3HCDH_N

Protein Analysis

922

Amino Acids

105.6

Weight (kDa)

8.8

Isoelectric Point (pI)

49.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 11 - 93 3.6e-21 Rx N-terminal domain
NB-ARC PF00931 175 - 350 6.1e-47 NB-ARC domain
WHD_DRP PF23559 437 - 508 4.1e-18 Disease resistance protein Winged helix domain
LRR_14 PF23598 554 - 877 2.2e-38 Leucine-rich repeat region
LRR_8 PF13855 581 - 635 1.9e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015035)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15290 AT3G15290
fragaria_vesca FvH4_3g19690
malus_domestica MD11G1251600.v1.1
prunus_persica Prupe.4G177500_v2.0.a1 Prupe.4G177500_v2.0.a1
pyrus_communis pycom11g22290
rosa_chinensis RchiOBHm_Chr5g0032991
rosa_laevigata RLG00000033441
rosa_multiflora Rmu_sc0000986.1_g000019
rosa_roxburghii Rroxscaffold_1G00047050
rosa_rugosa Rorug05G0134700
rosa_samantha Rh5AG227600 Rh5BG229100 Rh5DG233800
rosa_wichuraiana Rw5G020840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2165
Acc36I ACCTGC 1 cut(s) 1956
AccB7I CCANNNNNTGG 1 cut(s) 1099
AccI GTMKAC 2 cut(s) 280, 1529
AccII CGCG 1 cut(s) 2266
AciI CCGC 4 cut(s) 152, 784, 1513, 1873
AclWI GGATC 4 cut(s) 1179, 2694, 2743, 2756
AcoI YGGCCR 2 cut(s) 415, 2400
AcsI RAATTY 6 cut(s) 242, 766, 1219, 1547, 1822, 2497
AcuI CTGAAG 2 cut(s) 195, 462
AcyI GRCGYC 1 cut(s) 1867
AfaI GTAC 7 cut(s) 407, 705, 758, 1474, 2340, 2555, 2700
AfiI CCNNNNNNNGG 2 cut(s) 1099, 1123
AflII CTTAAG 1 cut(s) 2114
AflIII ACRYGT 3 cut(s) 255, 852, 1692
AhlI ACTAGT 1 cut(s) 805
AjiI CACGTC 1 cut(s) 853
AjnI CCWGG 2 cut(s) 834, 2634
Alw21I GWGCWC 2 cut(s) 1023, 1434
Alw26I GTCTC 7 cut(s) 149, 201, 646, 1004, 1874, 2173, 2352
AlwI GGATC 4 cut(s) 1179, 2694, 2743, 2756
Ama87I CYCGRG 1 cut(s) 1853
AoxI GGCC 7 cut(s) 12, 415, 1114, 1481, 2400, 2632, 2763
ApeKI GCWGC 1 cut(s) 295
ApoI RAATTY 6 cut(s) 242, 766, 1219, 1547, 1822, 2497
ArsI GACNNNNNNTTYG 2 cut(s) 262, 294
Asp700I GAANNNNTTC 5 cut(s) 137, 447, 1037, 1116, 2232
AspLEI GCGC 3 cut(s) 314, 1953, 2268
AspS9I GGNCC 3 cut(s) 28, 1180, 2632
AsuC2I CCSGG 1 cut(s) 1650
AsuHPI GGTGA 3 cut(s) 658, 974, 2548
AsuII TTCGAA 2 cut(s) 715, 2272
AvaI CYCGRG 1 cut(s) 1853
AvaII GGWCC 2 cut(s) 28, 1180
BalI TGGCCA 2 cut(s) 417, 2402
BamHI GGATCC 1 cut(s) 2748
BanII GRGCYC 2 cut(s) 1023, 1434
BarI GAAGNNNNNNTAC 4 cut(s) 740, 772, 2361, 2393
BbsI GAAGAC 3 cut(s) 504, 1146, 2591
Bbv12I GWGCWC 2 cut(s) 1023, 1434
BbvI GCAGC 1 cut(s) 282
BccI CCATC 7 cut(s) 532, 537, 1282, 1559, 1619, 1962, 2029
BceAI ACGGC 1 cut(s) 1775
BcgI CGANNNNNNTGC 2 cut(s) 1355, 1389
BciT130I CCWGG 2 cut(s) 836, 2636
BciVI GTATCC 1 cut(s) 2074
BclI TGATCA 1 cut(s) 375
BcnI CCSGG 1 cut(s) 1650
BcoDI GTCTC 7 cut(s) 149, 201, 646, 1004, 1874, 2173, 2352
BcuI ACTAGT 1 cut(s) 805
BfmI CTRYAG 1 cut(s) 2327
BfoI RGCGCY 1 cut(s) 315
BfrI CTTAAG 1 cut(s) 2114
BfuAI ACCTGC 1 cut(s) 1956
BfuI GTATCC 1 cut(s) 2074
BglII AGATCT 1 cut(s) 1587
BisI GCNGC 2 cut(s) 296, 784
BlsI GCNGC 2 cut(s) 297, 785
BmcAI AGTACT 1 cut(s) 758
Bme1390I CCNGG 3 cut(s) 836, 1650, 2636
Bme18I GGWCC 2 cut(s) 28, 1180
BmeT110I CYCGRG 1 cut(s) 1853
BmgBI CACGTC 1 cut(s) 853
BmgT120I GGNCC 3 cut(s) 28, 1180, 2632
BmiI GGNNCC 2 cut(s) 1181, 2750
BmrFI CCNGG 3 cut(s) 836, 1650, 2636
BmsI GCATC 8 cut(s) 139, 210, 1216, 1488, 1500, 1777, 2130, 2529
BpiI GAAGAC 3 cut(s) 504, 1146, 2591
BpmI CTGGAG 1 cut(s) 2498
Bpu14I TTCGAA 2 cut(s) 715, 2272
BpuEI CTTGAG 2 cut(s) 80, 1831
BpuMI CCSGG 1 cut(s) 1650
BsaHI GRCGYC 1 cut(s) 1867
BsaI GGTCTC 2 cut(s) 149, 2173
BsaJI CCNNGG 1 cut(s) 2312
BsaWI WCCGGW 1 cut(s) 2550
BsaXI ACNNNNNCTCC 2 cut(s) 1456, 1486
Bsc4I CCNNNNNNNGG 2 cut(s) 1099, 1123
Bse1I ACTGG 7 cut(s) 510, 1173, 1725, 1981, 2237, 2293, 2403
Bse3DI GCAATG 3 cut(s) 1223, 1288, 1643
BseBI CCWGG 2 cut(s) 836, 2636
BseDI CCNNGG 1 cut(s) 2312
BseGI GGATG 6 cut(s) 154, 548, 559, 1700, 1768, 2738
BseLI CCNNNNNNNGG 2 cut(s) 1099, 1123
BseMI GCAATG 3 cut(s) 1223, 1288, 1643
BseMII CTCAG 2 cut(s) 1062, 2520
BseNI ACTGG 7 cut(s) 510, 1173, 1725, 1981, 2237, 2293, 2403
BseRI GAGGAG 3 cut(s) 131, 441, 1644
BseXI GCAGC 1 cut(s) 282
BsgI GTGCAG 2 cut(s) 281, 482
Bsh1236I CGCG 1 cut(s) 2266
BshFI GGCC 7 cut(s) 14, 417, 1116, 1483, 2402, 2634, 2765
BsiHKAI GWGCWC 2 cut(s) 1023, 1434
BsiHKCI CYCGRG 1 cut(s) 1853
BsiSI CCGG 2 cut(s) 1649, 2551
BslFI GGGAC 1 cut(s) 1193
BslI CCNNNNNNNGG 2 cut(s) 1099, 1123
BsmAI GTCTC 7 cut(s) 149, 201, 646, 1004, 1874, 2173, 2352
BsmBI CGTCTC 1 cut(s) 1874
BsmFI GGGAC 1 cut(s) 1193
BsmI GAATGC 2 cut(s) 778, 1037
BsnI GGCC 7 cut(s) 14, 417, 1116, 1483, 2402, 2634, 2765
Bso31I GGTCTC 2 cut(s) 149, 2173
BsoBI CYCGRG 1 cut(s) 1853
Bsp119I TTCGAA 2 cut(s) 715, 2272
Bsp1286I GDGCHC 2 cut(s) 1023, 1434
Bsp1407I TGTACA 1 cut(s) 2338
Bsp143I GATC 6 cut(s) 375, 1171, 1504, 1587, 2686, 2748
BspACI CCGC 4 cut(s) 152, 784, 1513, 1873
BspANI GGCC 7 cut(s) 14, 417, 1116, 1483, 2402, 2634, 2765
BspCNI CTCAG 2 cut(s) 1063, 2519
BspFNI CGCG 1 cut(s) 2266
BspHI TCATGA 1 cut(s) 1254
BspLI GGNNCC 2 cut(s) 1181, 2750
BspMAI CTGCAG 1 cut(s) 2331
BspMI ACCTGC 1 cut(s) 1956
BspPI GGATC 4 cut(s) 1179, 2694, 2743, 2756
BspQI GCTCTTC 1 cut(s) 552
BspT104I TTCGAA 2 cut(s) 715, 2272
BspTI CTTAAG 1 cut(s) 2114
BspTNI GGTCTC 2 cut(s) 149, 2173
BsrDI GCAATG 3 cut(s) 1223, 1288, 1643
BsrGI TGTACA 1 cut(s) 2338
BsrI ACTGG 7 cut(s) 510, 1173, 1725, 1981, 2237, 2293, 2403
BssECI CCNNGG 1 cut(s) 2312
BssMI GATC 6 cut(s) 375, 1171, 1504, 1587, 2686, 2748
BssNI GRCGYC 1 cut(s) 1867
BssT1I CCWWGG 1 cut(s) 2312
Bst2UI CCWGG 2 cut(s) 836, 2636
Bst4CI ACNGT 3 cut(s) 410, 574, 2590
Bst6I CTCTTC 8 cut(s) 552, 644, 747, 1578, 1677, 2217, 2364, 2439
BstACI GRCGYC 1 cut(s) 1867
BstAFI CTTAAG 1 cut(s) 2114
BstAPI GCANNNNNTGC 1 cut(s) 2047
BstAUI TGTACA 1 cut(s) 2338
BstBI TTCGAA 2 cut(s) 715, 2272
BstC8I GCNNGC 4 cut(s) 199, 952, 1090, 1521
BstDEI CTNAG 7 cut(s) 125, 211, 1071, 1158, 1905, 2506, 2694
BstF5I GGATG 6 cut(s) 154, 548, 559, 1700, 1768, 2738
BstFNI CGCG 1 cut(s) 2266
BstH2I RGCGCY 1 cut(s) 315
BstHHI GCGC 3 cut(s) 314, 1953, 2268
BstKTI GATC 6 cut(s) 378, 1174, 1507, 1590, 2689, 2751
BstMAI GTCTC 7 cut(s) 149, 201, 646, 1004, 1874, 2173, 2352
BstMBI GATC 6 cut(s) 375, 1171, 1504, 1587, 2686, 2748
BstMWI GCNNNNNNNGC 5 cut(s) 11, 20, 597, 1948, 2047
BstNI CCWGG 2 cut(s) 836, 2636
BstNSI RCATGY 4 cut(s) 259, 954, 1450, 1696
BstSCI CCNGG 3 cut(s) 834, 1648, 2634
BstSFI CTRYAG 1 cut(s) 2327
BstUI CGCG 1 cut(s) 2266
BstV1I GCAGC 1 cut(s) 282
BstV2I GAAGAC 3 cut(s) 504, 1146, 2591
BstX2I RGATCY 2 cut(s) 1587, 2748
BstYI RGATCY 2 cut(s) 1587, 2748
BsuI GTATCC 1 cut(s) 2074
BsuRI GGCC 7 cut(s) 14, 417, 1116, 1483, 2402, 2634, 2765
BtrI CACGTC 1 cut(s) 853
BtsCI GGATG 6 cut(s) 154, 548, 559, 1700, 1768, 2738
BtsI GCAGTG 1 cut(s) 2324
BtsIMutI CAGTG 9 cut(s) 1166, 1270, 1718, 1974, 2244, 2324, 2396, 2514, 2595
BveI ACCTGC 1 cut(s) 1956
Cac8I GCNNGC 4 cut(s) 199, 952, 1090, 1521
CciI TCATGA 1 cut(s) 1254
CfoI GCGC 3 cut(s) 314, 1953, 2268
Cfr13I GGNCC 3 cut(s) 28, 1180, 2632
CseI GACGC 2 cut(s) 1856, 1902
CsiI ACCWGGT 1 cut(s) 834
Csp6I GTAC 7 cut(s) 406, 704, 757, 1473, 2339, 2554, 2699
CviQI GTAC 7 cut(s) 406, 704, 757, 1473, 2339, 2554, 2699
DdeI CTNAG 7 cut(s) 125, 211, 1071, 1158, 1905, 2506, 2694
DpnI GATC 6 cut(s) 377, 1173, 1506, 1589, 2688, 2750
DpnII GATC 6 cut(s) 375, 1171, 1504, 1587, 2686, 2748
DraI TTTAAA 1 cut(s) 1821
DrdI GACNNNNNNGTC 1 cut(s) 2165
DseDI GACNNNNNNGTC 1 cut(s) 2165
EaeI YGGCCR 2 cut(s) 415, 2400
Eam1104I CTCTTC 8 cut(s) 552, 644, 747, 1578, 1677, 2217, 2364, 2439
EarI CTCTTC 8 cut(s) 552, 644, 747, 1578, 1677, 2217, 2364, 2439
Ecl136II GAGCTC 2 cut(s) 1021, 1432
Eco130I CCWWGG 1 cut(s) 2312
Eco147I AGGCCT 1 cut(s) 1116
Eco24I GRGCYC 2 cut(s) 1023, 1434
Eco31I GGTCTC 2 cut(s) 149, 2173
Eco32I GATATC 2 cut(s) 232, 2716
Eco47I GGWCC 2 cut(s) 28, 1180
Eco53kI GAGCTC 2 cut(s) 1021, 1432
Eco57I CTGAAG 2 cut(s) 195, 462
Eco88I CYCGRG 1 cut(s) 1853
EcoICRI GAGCTC 2 cut(s) 1021, 1432
EcoRI GAATTC 1 cut(s) 242
EcoRII CCWGG 2 cut(s) 834, 2634
EcoRV GATATC 2 cut(s) 232, 2716
EcoT14I CCWWGG 1 cut(s) 2312
EcoT22I ATGCAT 2 cut(s) 1503, 2434
EcoT38I GRGCYC 2 cut(s) 1023, 1434
ErhI CCWWGG 1 cut(s) 2312
Esp3I CGTCTC 1 cut(s) 1874
FaqI GGGAC 1 cut(s) 1193
FauI CCCGC 1 cut(s) 1506
FauNDI CATATG 1 cut(s) 2434
FbaI TGATCA 1 cut(s) 375
FblI GTMKAC 2 cut(s) 280, 1529
Fnu4HI GCNGC 2 cut(s) 296, 784
FokI GGATG 6 cut(s) 161, 555, 566, 1687, 1775, 2725
FriOI GRGCYC 2 cut(s) 1023, 1434
Fsp4HI GCNGC 2 cut(s) 296, 784
GlaI GCGC 3 cut(s) 313, 1952, 2267
GluI GCNGC 2 cut(s) 296, 784
GsuI CTGGAG 1 cut(s) 2498
HaeII RGCGCY 1 cut(s) 315
HaeIII GGCC 7 cut(s) 14, 417, 1116, 1483, 2402, 2634, 2765
HapII CCGG 2 cut(s) 1649, 2551
HgaI GACGC 2 cut(s) 1856, 1902
HhaI GCGC 3 cut(s) 314, 1953, 2268
Hin1I GRCGYC 1 cut(s) 1867
Hin6I GCGC 3 cut(s) 312, 1951, 2266
HinP1I GCGC 3 cut(s) 312, 1951, 2266
HincII GTYRAC 4 cut(s) 281, 811, 1530, 2566
HindII GTYRAC 4 cut(s) 281, 811, 1530, 2566
HindIII AAGCTT 3 cut(s) 135, 718, 1981
HpaI GTTAAC 1 cut(s) 811
HpaII CCGG 2 cut(s) 1649, 2551
HphI GGTGA 3 cut(s) 658, 974, 2548
Hpy166II GTNNAC 8 cut(s) 281, 706, 811, 982, 1530, 1798, 2255, 2566
Hpy8I GTNNAC 8 cut(s) 281, 706, 811, 982, 1530, 1798, 2255, 2566
Hpy99I CGWCG 2 cut(s) 1602, 1918
HpyCH4III ACNGT 3 cut(s) 410, 574, 2590
HpyCH4IV ACGT 1 cut(s) 852
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 20, 597, 1948, 2047
HpyF3I CTNAG 7 cut(s) 125, 211, 1071, 1158, 1905, 2506, 2694
HpySE526I ACGT 1 cut(s) 852
Hsp92I GRCGYC 1 cut(s) 1867
HspAI GCGC 3 cut(s) 312, 1951, 2266
Ksp22I TGATCA 1 cut(s) 375
KspAI GTTAAC 1 cut(s) 811
Kzo9I GATC 6 cut(s) 375, 1171, 1504, 1587, 2686, 2748
LguI GCTCTTC 1 cut(s) 552
LmnI GCTCC 5 cut(s) 319, 428, 1018, 1429, 1516
Lsp1109I GCAGC 1 cut(s) 282
LweI GCATC 8 cut(s) 139, 210, 1216, 1488, 1500, 1777, 2130, 2529
MabI ACCWGGT 1 cut(s) 834
MaeII ACGT 1 cut(s) 852
MaeIII GTNAC 6 cut(s) 665, 801, 925, 1164, 1393, 1449
MalI GATC 6 cut(s) 377, 1173, 1506, 1589, 2688, 2750
MboI GATC 6 cut(s) 375, 1171, 1504, 1587, 2686, 2748
MfeI CAATTG 4 cut(s) 661, 1280, 2172, 2640
MflI RGATCY 2 cut(s) 1587, 2748
MhlI GDGCHC 2 cut(s) 1023, 1434
MlsI TGGCCA 2 cut(s) 417, 2402
MluNI TGGCCA 2 cut(s) 417, 2402
MlyI GAGTC 5 cut(s) 485, 830, 1741, 1865, 2101
MmeI TCCRAC 1 cut(s) 2755
Mox20I TGGCCA 2 cut(s) 417, 2402
Mph1103I ATGCAT 2 cut(s) 1503, 2434
MroXI GAANNNNTTC 5 cut(s) 137, 447, 1037, 1116, 2232
MscI TGGCCA 2 cut(s) 417, 2402
MslI CAYNNNNRTG 1 cut(s) 258
Msp20I TGGCCA 2 cut(s) 417, 2402
MspCI CTTAAG 1 cut(s) 2114
MspI CCGG 2 cut(s) 1649, 2551
MspR9I CCNGG 3 cut(s) 836, 1650, 2636
MunI CAATTG 4 cut(s) 661, 1280, 2172, 2640
Mva1269I GAATGC 2 cut(s) 778, 1037
MvaI CCWGG 2 cut(s) 836, 2636
MvnI CGCG 1 cut(s) 2266
MwoI GCNNNNNNNGC 5 cut(s) 11, 20, 597, 1948, 2047
NciI CCSGG 1 cut(s) 1650
NdeI CATATG 1 cut(s) 2434
NdeII GATC 6 cut(s) 375, 1171, 1504, 1587, 2686, 2748
NlaIV GGNNCC 2 cut(s) 1181, 2750
NmeAIII GCCGAG 1 cut(s) 1561
NmuCI GTSAC 2 cut(s) 1164, 1393
NsiI ATGCAT 2 cut(s) 1503, 2434
NspI RCATGY 4 cut(s) 259, 954, 1450, 1696
NspV TTCGAA 2 cut(s) 715, 2272
PaeI GCATGC 1 cut(s) 954
PagI TCATGA 1 cut(s) 1254
PceI AGGCCT 1 cut(s) 1116
PciI ACATGT 2 cut(s) 255, 1692
PciSI GCTCTTC 1 cut(s) 552
PctI GAATGC 2 cut(s) 778, 1037
PdmI GAANNNNTTC 5 cut(s) 137, 447, 1037, 1116, 2232
PfeI GAWTC 8 cut(s) 397, 918, 1079, 1663, 1731, 1901, 2069, 2185
PflFI GACNNNGTC 1 cut(s) 1184
PflMI CCANNNNNTGG 1 cut(s) 1099
PkrI GCNGC 2 cut(s) 297, 785
PleI GAGTC 5 cut(s) 484, 829, 1741, 1864, 2101
PpsI GAGTC 5 cut(s) 484, 829, 1741, 1864, 2101
PscI ACATGT 2 cut(s) 255, 1692
Psp124BI GAGCTC 2 cut(s) 1023, 1434
Psp6I CCWGG 2 cut(s) 834, 2634
PspGI CCWGG 2 cut(s) 834, 2634
PspN4I GGNNCC 2 cut(s) 1181, 2750
PspPI GGNCC 3 cut(s) 28, 1180, 2632
PstI CTGCAG 1 cut(s) 2331
PsuI RGATCY 2 cut(s) 1587, 2748
PsyI GACNNNGTC 1 cut(s) 1184
RsaI GTAC 7 cut(s) 407, 705, 758, 1474, 2340, 2555, 2700
RsaNI GTAC 7 cut(s) 406, 704, 757, 1473, 2339, 2554, 2699
RseI CAYNNNNRTG 1 cut(s) 258
SacI GAGCTC 2 cut(s) 1023, 1434
SalI GTCGAC 2 cut(s) 279, 1528
SapI GCTCTTC 1 cut(s) 552
SatI GCNGC 2 cut(s) 296, 784
Sau3AI GATC 6 cut(s) 375, 1171, 1504, 1587, 2686, 2748
Sau96I GGNCC 3 cut(s) 28, 1180, 2632
ScaI AGTACT 1 cut(s) 758
SchI GAGTC 5 cut(s) 485, 830, 1741, 1865, 2101
ScrFI CCNGG 3 cut(s) 836, 1650, 2636
SduI GDGCHC 2 cut(s) 1023, 1434
SexAI ACCWGGT 1 cut(s) 834
SfaNI GCATC 8 cut(s) 139, 210, 1216, 1488, 1500, 1777, 2130, 2529
SfcI CTRYAG 1 cut(s) 2327
SfuI TTCGAA 2 cut(s) 715, 2272
SinI GGWCC 2 cut(s) 28, 1180
SmiI ATTTAAAT 1 cut(s) 1821
SmiMI CAYNNNNRTG 1 cut(s) 258
SmlI CTYRAG 3 cut(s) 59, 1810, 2114
SmoI CTYRAG 3 cut(s) 59, 1810, 2114
SpeI ACTAGT 1 cut(s) 805
SphI GCATGC 1 cut(s) 954
SseBI AGGCCT 1 cut(s) 1116
SsiI CCGC 4 cut(s) 152, 784, 1513, 1873
SspI AATATT 1 cut(s) 2029
SstI GAGCTC 2 cut(s) 1023, 1434
StuI AGGCCT 1 cut(s) 1116
StyD4I CCNGG 3 cut(s) 834, 1648, 2634
StyI CCWWGG 1 cut(s) 2312
SwaI ATTTAAAT 1 cut(s) 1821
TaaI ACNGT 3 cut(s) 410, 574, 2590
TaiI ACGT 1 cut(s) 855
TaqI TCGA 7 cut(s) 280, 715, 916, 934, 1529, 1729, 2272
TaqII GACCGA 1 cut(s) 2679
TatI WGTACW 2 cut(s) 756, 2338
TauI GCSGC 1 cut(s) 786
TfiI GAWTC 8 cut(s) 397, 918, 1079, 1663, 1731, 1901, 2069, 2185
TscAI CASTG 9 cut(s) 1173, 1270, 1725, 1981, 2244, 2331, 2403, 2514, 2595
TseFI GTSAC 2 cut(s) 1164, 1393
TseI GCWGC 1 cut(s) 295
Tsp45I GTSAC 2 cut(s) 1164, 1393
TspGWI ACGGA 2 cut(s) 41, 1430
TspRI CASTG 9 cut(s) 1173, 1270, 1725, 1981, 2244, 2331, 2403, 2514, 2595
Tth111I GACNNNGTC 1 cut(s) 1184
Van91I CCANNNNNTGG 1 cut(s) 1099
Vha464I CTTAAG 1 cut(s) 2114
VpaK11BI GGWCC 2 cut(s) 28, 1180
XapI RAATTY 6 cut(s) 242, 766, 1219, 1547, 1822, 2497
XbaI TCTAGA 4 cut(s) 844, 1750, 1987, 2360
XceI RCATGY 4 cut(s) 259, 954, 1450, 1696
XcmI CCANNNNNNNNNTGG 2 cut(s) 324, 832
XmiI GTMKAC 2 cut(s) 280, 1529
XmnI GAANNNNTTC 5 cut(s) 137, 447, 1037, 1116, 2232
ZrmI AGTACT 1 cut(s) 758
Zsp2I ATGCAT 2 cut(s) 1503, 2434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.