MD11G1277400.v1.1

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0016351
Physical Location & Seq
Reverse (-)
39457172 .. 39459936
2765 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1277400.v1.1.491

Sequence Viewer

Length: 1416 bp
ATGGCGCTCCCATTGAACCTCCAACCTGACGAGGCGAGCCCTGATTGGCTCAGCAAGGGTGACAATGCTTGGCAACTCACGGCTGCCACGCTCGTTGGCATGCAGAGCGTGCCAGGGCTTGTCATTCTCTACGGAAGCATAGTGAAAAAGAAGTGGGCGGTGAACTCCGCCTTCATGGCTCTGTACGCCTTCGCAGCAGTCTTGGTCTGTTGGGTCGGCTGGGGCTACCAAATGTCGTTCGGGGACCAGCTCTTCCCGTTCCTCGGCCGACCCAACGTGGCCATGGACCAAGCCTTCCTGCTGAAAAGAACATTCAGTGGGTTTCTTCCAAATGCAACTATGATTTATTTTCAGTTCGTGTTTGCGGCCATTACGTTGATTTTGATCGCTGGGGCTTTGTTGGGGAGGATGAACTTCCATGCATGGATGTTATTTGTACCCCTTTGGTTGACATTTTCTTACACAATTACTTGCTATAGTATTTTTTACCCCAATGGCTGGTTGTATAAGATGGGGCTTATTGACTACTCTGGCGGCTACGTCATTCATCTCTCTTCTGGTGTTGCTGGTTTCACTGCTGCGTACTGGGTTGGGCCAAGGGCAACCAATGACAGGGAGAGATTTCCACCAAACAACATTCTGCTGATGCTTGCTGGGGCGGGCCTACTTTGGATGGGGTGGAGTGGATTTAACGGTGGAGATCCTTACATGGTGAGCACAGACGCATCTCTGGCCGTCCTCAACACCCACGTGTGCACTGCCACTAGCTTGCTGACGTGGCTCTTGCTTGACATTGCTTTCTTCGGGAAGCCCTCTGTGATCGGTGCCACTCAAGGCATGATCACTGGCTTAGTTTGCATTACTCCTGCTGCAGGAGTGGTACAAGGTTGGGCAGCAATCATAATGGGAATGATGTCAGGAAGCATCCCATGGTACACTATGATGGTCCTCCACAAGAAAATCGGCATCCTAAAGCAAGTCGATGACACCATGGCCGTGTTCCACACCCACGCCGTGGCCGGAAGCCTAGGTGGCATCCTTGCCGGCTTCTTTGCCCACCCGAAGCTCTGCCGTATATTCTACTTAACCGACAAGTGGGAACACTACATTGGCCTTGCCTATGGATTGCAAAGTGGCCATGTCGGTGCAGGGTTCAAGCAAATGGGAATCCAATTCCTAGGAGTAGCGTTTGTGGTTACTGTCAATGTGATCATCACTAGCATCATTTGCTTGTTCTTGAGGCTTTTCATTCCACTCAGGCTCAACGACGACGAGTTGCAGGTCGGTGATGACGCGATCCACGGCGAGGAGGCTTATGCATTGTGGGGAGATGGAGAGAAGTATGATGGTTCCAAGCATAACTCAGTTTATGGAATGGAAGAGTTTCCCATGAATGCGCCTAAAGCTGAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

472

Amino Acids

51.58

Weight (kDa)

6.24

Isoelectric Point (pI)

32.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 23 - 439 1.2e-79 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016351)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30820
malus_domestica MD11G1277400.v1.1
prunus_persica Prupe.8G233700_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0262941
rosa_laevigata RLG00000014406
rosa_multiflora Rmu_sc0000596.1_g000063 Rmu_sc0006493.1_g000013
rosa_roxburghii Rroxscaffold_177G00434070 Rroxscaffold_7G00204870
rosa_rugosa Rorug06G0001700
rosa_samantha Rh6AG121000 Rh6BG114700 Rh6CG113100 Rh6DG102400
rosa_wichuraiana Rw6G010390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1270
AccB1I GGYRCC 1 cut(s) 824
AccB7I CCANNNNNTGG 2 cut(s) 498, 1015
AccII CGCG 1 cut(s) 1295
AciI CCGC 5 cut(s) 158, 168, 365, 534, 659
AclWI GGATC 2 cut(s) 695, 1291
AcoI YGGCCR 7 cut(s) 265, 279, 366, 732, 993, 1017, 1135
AcvI CACGTG 1 cut(s) 751
AdeI CACNNNGTG 1 cut(s) 1015
AfaI GTAC 5 cut(s) 185, 438, 584, 882, 935
AfiI CCNNNNNNNGG 7 cut(s) 263, 498, 612, 872, 1015, 1095, 1306
AflIII ACRYGT 1 cut(s) 750
AgsI TTSAA 2 cut(s) 16, 1156
AjiI CACGTC 1 cut(s) 777
AjnI CCWGG 1 cut(s) 112
AjuI GAANNNNNNNTTGG 2 cut(s) 1092, 1124
AleI CACNNNNGTG 1 cut(s) 749
AluBI AGCT 4 cut(s) 250, 768, 1066, 1406
AluI AGCT 4 cut(s) 250, 768, 1066, 1406
Alw21I GWGCWC 2 cut(s) 719, 758
Alw44I GTGCAC 1 cut(s) 754
AlwI GGATC 2 cut(s) 695, 1291
ApaLI GTGCAC 1 cut(s) 754
ApeKI GCWGC 5 cut(s) 83, 194, 578, 869, 893
Asp700I GAANNNNTTC 1 cut(s) 1383
AspA2I CCTAGG 2 cut(s) 1027, 1177
AspLEI GCGC 2 cut(s) 7, 1399
AspS9I GGNCC 5 cut(s) 244, 286, 593, 661, 946
AsuHPI GGTGA 4 cut(s) 71, 172, 724, 1298
AvaII GGWCC 3 cut(s) 244, 286, 946
AvrII CCTAGG 2 cut(s) 1027, 1177
BaeGI GKGCMC 1 cut(s) 758
BalI TGGCCA 2 cut(s) 281, 1137
BanI GGYRCC 1 cut(s) 824
BanII GRGCYC 1 cut(s) 41
BbrPI CACGTG 1 cut(s) 751
Bbv12I GWGCWC 2 cut(s) 719, 758
BbvI GCAGC 5 cut(s) 70, 206, 565, 856, 905
BccI CCATC 5 cut(s) 505, 667, 937, 1325, 1340
BceAI ACGGC 6 cut(s) 96, 719, 980, 998, 1056, 1318
BciT130I CCWGG 1 cut(s) 114
BclI TGATCA 2 cut(s) 840, 1209
BfaI CTAG 4 cut(s) 765, 1028, 1178, 1218
BfmI CTRYAG 2 cut(s) 475, 870
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 1270
BglI GCCNNNNNGGC 2 cut(s) 176, 1032
BisI GCNGC 7 cut(s) 84, 195, 366, 535, 579, 870, 894
BlnI CCTAGG 2 cut(s) 1027, 1177
BlpI GCTNAGC 1 cut(s) 50
BlsI GCNGC 7 cut(s) 85, 196, 367, 536, 580, 871, 895
Bme1390I CCNGG 1 cut(s) 114
Bme18I GGWCC 3 cut(s) 244, 286, 946
BmgBI CACGTC 1 cut(s) 777
BmgT120I GGNCC 5 cut(s) 244, 286, 593, 661, 946
BmiI GGNNCC 3 cut(s) 245, 826, 1351
BmrFI CCNGG 1 cut(s) 114
BmrI ACTGGG 1 cut(s) 595
BmsI GCATC 6 cut(s) 636, 734, 933, 975, 1044, 1230
BmuI ACTGGG 1 cut(s) 595
Bpu1102I GCTNAGC 1 cut(s) 50
BpuEI CTTGAG 2 cut(s) 816, 1258
BsaAI YACGTR 1 cut(s) 751
BsaBI GATNNNNATC 1 cut(s) 383
Bsc4I CCNNNNNNNGG 7 cut(s) 263, 498, 612, 872, 1015, 1095, 1306
Bse118I RCCGGY 1 cut(s) 1043
Bse1I ACTGG 2 cut(s) 590, 850
Bse3DI GCAATG 1 cut(s) 792
Bse8I GATNNNNATC 1 cut(s) 383
BseBI CCWGG 1 cut(s) 114
BseGI GGATG 6 cut(s) 414, 432, 678, 924, 966, 1035
BseJI GATNNNNATC 1 cut(s) 383
BseLI CCNNNNNNNGG 7 cut(s) 263, 498, 612, 872, 1015, 1095, 1306
BseMI GCAATG 1 cut(s) 792
BseMII CTCAG 3 cut(s) 64, 1270, 1377
BseNI ACTGG 2 cut(s) 590, 850
BseRI GAGGAG 1 cut(s) 1322
BseSI GKGCMC 1 cut(s) 758
BseX3I CGGCCG 1 cut(s) 265
BseXI GCAGC 5 cut(s) 70, 206, 565, 856, 905
BseYI CCCAGC 3 cut(s) 219, 389, 653
BsgI GTGCAG 1 cut(s) 1167
Bsh1236I CGCG 1 cut(s) 1295
Bsh1285I CGRYCG 1 cut(s) 268
BshNI GGYRCC 1 cut(s) 824
BsiEI CGRYCG 1 cut(s) 268
BsiHKAI GWGCWC 2 cut(s) 719, 758
BsiSI CCGG 2 cut(s) 1020, 1044
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 7 cut(s) 263, 498, 612, 872, 1015, 1095, 1306
BsmFI GGGAC 1 cut(s) 257
BsmI GAATGC 1 cut(s) 1399
Bsp1286I GDGCHC 3 cut(s) 41, 719, 758
Bsp143I GATC 6 cut(s) 384, 700, 819, 840, 1209, 1296
Bsp1720I GCTNAGC 1 cut(s) 50
Bsp19I CCATGG 3 cut(s) 282, 929, 990
BspACI CCGC 5 cut(s) 158, 168, 365, 534, 659
BspCNI CTCAG 3 cut(s) 63, 1269, 1376
BspFNI CGCG 1 cut(s) 1295
BspLI GGNNCC 3 cut(s) 245, 826, 1351
BspMAI CTGCAG 1 cut(s) 874
BspMI ACCTGC 1 cut(s) 1270
BspPI GGATC 2 cut(s) 695, 1291
BspQI GCTCTTC 1 cut(s) 257
BspT107I GGYRCC 1 cut(s) 824
BsrDI GCAATG 1 cut(s) 792
BsrFI RCCGGY 1 cut(s) 1043
BsrI ACTGG 2 cut(s) 590, 850
BssAI RCCGGY 1 cut(s) 1043
BssMI GATC 6 cut(s) 384, 700, 819, 840, 1209, 1296
BssT1I CCWWGG 6 cut(s) 282, 596, 929, 990, 1027, 1177
Bst2UI CCWGG 1 cut(s) 114
Bst4CI ACNGT 2 cut(s) 695, 1201
Bst6I CTCTTC 3 cut(s) 257, 559, 1374
BstAPI GCANNNNNTGC 2 cut(s) 109, 1227
BstBAI YACGTR 1 cut(s) 751
BstC8I GCNNGC 7 cut(s) 37, 101, 110, 651, 661, 770, 1045
BstDEI CTNAG 4 cut(s) 50, 850, 1256, 1363
BstDSI CCRYGG 5 cut(s) 282, 929, 990, 1014, 1300
BstENI CCTNNNNNAGG 1 cut(s) 870
BstF5I GGATG 6 cut(s) 414, 432, 678, 924, 966, 1035
BstFNI CGCG 1 cut(s) 1295
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 1399
BstKTI GATC 6 cut(s) 387, 703, 822, 843, 1212, 1299
BstMBI GATC 6 cut(s) 384, 700, 819, 840, 1209, 1296
BstMCI CGRYCG 1 cut(s) 268
BstNI CCWGG 1 cut(s) 114
BstNSI RCATGY 1 cut(s) 103
BstSCI CCNGG 1 cut(s) 112
BstSFI CTRYAG 2 cut(s) 475, 870
BstSLI GKGCMC 1 cut(s) 758
BstUI CGCG 1 cut(s) 1295
BstV1I GCAGC 5 cut(s) 70, 206, 565, 856, 905
BstX2I RGATCY 1 cut(s) 700
BstYI RGATCY 1 cut(s) 700
BstZI CGGCCG 1 cut(s) 265
BtgI CCRYGG 5 cut(s) 282, 929, 990, 1014, 1300
BtrI CACGTC 1 cut(s) 777
BtsCI GGATG 6 cut(s) 414, 432, 678, 924, 966, 1035
BtsI GCAGTG 2 cut(s) 573, 756
BtsIMutI CAGTG 4 cut(s) 322, 573, 756, 843
BveI ACCTGC 1 cut(s) 1270
Cac8I GCNNGC 7 cut(s) 37, 101, 110, 651, 661, 770, 1045
CfoI GCGC 2 cut(s) 7, 1399
Cfr10I RCCGGY 1 cut(s) 1043
Cfr13I GGNCC 5 cut(s) 244, 286, 593, 661, 946
CseI GACGC 2 cut(s) 731, 1301
Csp6I GTAC 5 cut(s) 184, 437, 583, 881, 934
CspCI CAANNNNNGTGG 2 cut(s) 76, 111
CviQI GTAC 5 cut(s) 184, 437, 583, 881, 934
DdeI CTNAG 4 cut(s) 50, 850, 1256, 1363
DpnI GATC 6 cut(s) 386, 702, 821, 842, 1211, 1298
DpnII GATC 6 cut(s) 384, 700, 819, 840, 1209, 1296
DraIII CACNNNGTG 1 cut(s) 1015
EaeI YGGCCR 7 cut(s) 265, 279, 366, 732, 993, 1017, 1135
EagI CGGCCG 1 cut(s) 265
Eam1104I CTCTTC 3 cut(s) 257, 559, 1374
EarI CTCTTC 3 cut(s) 257, 559, 1374
EciI GGCGGA 1 cut(s) 157
EclXI CGGCCG 1 cut(s) 265
Eco130I CCWWGG 6 cut(s) 282, 596, 929, 990, 1027, 1177
Eco24I GRGCYC 1 cut(s) 41
Eco47I GGWCC 3 cut(s) 244, 286, 946
Eco52I CGGCCG 1 cut(s) 265
Eco72I CACGTG 1 cut(s) 751
EcoNI CCTNNNNNAGG 1 cut(s) 870
EcoRII CCWGG 1 cut(s) 112
EcoT14I CCWWGG 6 cut(s) 282, 596, 929, 990, 1027, 1177
EcoT22I ATGCAT 2 cut(s) 424, 1321
EcoT38I GRGCYC 1 cut(s) 41
ErhI CCWWGG 6 cut(s) 282, 596, 929, 990, 1027, 1177
FaqI GGGAC 1 cut(s) 257
FauI CCCGC 1 cut(s) 652
FbaI TGATCA 2 cut(s) 840, 1209
Fnu4HI GCNGC 7 cut(s) 84, 195, 366, 535, 579, 870, 894
FokI GGATG 6 cut(s) 421, 439, 685, 911, 953, 1022
FriOI GRGCYC 1 cut(s) 41
Fsp4HI GCNGC 7 cut(s) 84, 195, 366, 535, 579, 870, 894
FspBI CTAG 4 cut(s) 765, 1028, 1178, 1218
GlaI GCGC 2 cut(s) 6, 1398
GluI GCNGC 7 cut(s) 84, 195, 366, 535, 579, 870, 894
GsaI CCCAGC 3 cut(s) 223, 393, 657
HaeII RGCGCY 1 cut(s) 8
HapII CCGG 2 cut(s) 1020, 1044
HgaI GACGC 2 cut(s) 731, 1301
HhaI GCGC 2 cut(s) 7, 1399
Hin6I GCGC 2 cut(s) 5, 1397
HinP1I GCGC 2 cut(s) 5, 1397
HincII GTYRAC 1 cut(s) 450
HindII GTYRAC 1 cut(s) 450
HinfI GANTC 1 cut(s) 1167
HpaII CCGG 2 cut(s) 1020, 1044
HphI GGTGA 4 cut(s) 71, 172, 724, 1298
Hpy166II GTNNAC 4 cut(s) 163, 450, 756, 936
Hpy188III TCNNGA 3 cut(s) 805, 918, 1237
Hpy8I GTNNAC 4 cut(s) 163, 450, 756, 936
Hpy99I CGWCG 2 cut(s) 1271, 1274
HpyAV CCTTC 3 cut(s) 181, 199, 304
HpyCH4III ACNGT 2 cut(s) 695, 1201
HpyCH4IV ACGT 5 cut(s) 276, 374, 540, 750, 776
HpyF3I CTNAG 4 cut(s) 50, 850, 1256, 1363
HpySE526I ACGT 5 cut(s) 276, 374, 540, 750, 776
HspAI GCGC 2 cut(s) 5, 1397
KroI GCCGGC 1 cut(s) 1043
KroNI GCCGGC 1 cut(s) 1045
Ksp22I TGATCA 2 cut(s) 840, 1209
Kzo9I GATC 6 cut(s) 384, 700, 819, 840, 1209, 1296
LguI GCTCTTC 1 cut(s) 257
LmnI GCTCC 1 cut(s) 12
Lsp1109I GCAGC 5 cut(s) 70, 206, 565, 856, 905
LweI GCATC 6 cut(s) 636, 734, 933, 975, 1044, 1230
MaeI CTAG 4 cut(s) 765, 1028, 1178, 1218
MaeII ACGT 5 cut(s) 276, 374, 540, 750, 776
MaeIII GTNAC 2 cut(s) 59, 1195
MalI GATC 6 cut(s) 386, 702, 821, 842, 1211, 1298
MboI GATC 6 cut(s) 384, 700, 819, 840, 1209, 1296
MboII GAAGA 5 cut(s) 244, 317, 546, 793, 1391
MflI RGATCY 1 cut(s) 700
MhlI GDGCHC 3 cut(s) 41, 719, 758
MlsI TGGCCA 2 cut(s) 281, 1137
MluCI AATT 2 cut(s) 465, 1172
MluNI TGGCCA 2 cut(s) 281, 1137
MmeI TCCRAC 1 cut(s) 46
Mox20I TGGCCA 2 cut(s) 281, 1137
Mph1103I ATGCAT 2 cut(s) 424, 1321
MroNI GCCGGC 1 cut(s) 1043
MroXI GAANNNNTTC 1 cut(s) 1383
MscI TGGCCA 2 cut(s) 281, 1137
MseI TTAA 2 cut(s) 690, 1085
MslI CAYNNNNRTG 4 cut(s) 749, 941, 995, 1143
Msp20I TGGCCA 2 cut(s) 281, 1137
MspI CCGG 2 cut(s) 1020, 1044
MspR9I CCNGG 1 cut(s) 114
Mva1269I GAATGC 1 cut(s) 1399
MvaI CCWGG 1 cut(s) 114
MvnI CGCG 1 cut(s) 1295
NaeI GCCGGC 1 cut(s) 1045
NcoI CCATGG 3 cut(s) 282, 929, 990
NdeII GATC 6 cut(s) 384, 700, 819, 840, 1209, 1296
NgoMIV GCCGGC 1 cut(s) 1043
NlaIV GGNNCC 3 cut(s) 245, 826, 1351
NmeAIII GCCGAG 1 cut(s) 243
NmuCI GTSAC 1 cut(s) 59
NsiI ATGCAT 2 cut(s) 424, 1321
NspI RCATGY 1 cut(s) 103
OliI CACNNNNGTG 1 cut(s) 749
PaeI GCATGC 1 cut(s) 103
PciSI GCTCTTC 1 cut(s) 257
PctI GAATGC 1 cut(s) 1399
PdiI GCCGGC 1 cut(s) 1045
PdmI GAANNNNTTC 1 cut(s) 1383
PfeI GAWTC 1 cut(s) 1167
PflMI CCANNNNNTGG 2 cut(s) 498, 1015
PkrI GCNGC 7 cut(s) 85, 196, 367, 536, 580, 871, 895
PmaCI CACGTG 1 cut(s) 751
PmlI CACGTG 1 cut(s) 751
Ppu21I YACGTR 1 cut(s) 751
Psp6I CCWGG 1 cut(s) 112
PspCI CACGTG 1 cut(s) 751
PspFI CCCAGC 3 cut(s) 219, 389, 653
PspGI CCWGG 1 cut(s) 112
PspN4I GGNNCC 3 cut(s) 245, 826, 1351
PspPI GGNCC 5 cut(s) 244, 286, 593, 661, 946
PstI CTGCAG 1 cut(s) 874
PsuI RGATCY 1 cut(s) 700
RsaI GTAC 5 cut(s) 185, 438, 584, 882, 935
RsaNI GTAC 5 cut(s) 184, 437, 583, 881, 934
RseI CAYNNNNRTG 4 cut(s) 749, 941, 995, 1143
SapI GCTCTTC 1 cut(s) 257
SaqAI TTAA 2 cut(s) 690, 1085
SatI GCNGC 7 cut(s) 84, 195, 366, 535, 579, 870, 894
Sau3AI GATC 6 cut(s) 384, 700, 819, 840, 1209, 1296
Sau96I GGNCC 5 cut(s) 244, 286, 593, 661, 946
ScrFI CCNGG 1 cut(s) 114
SduI GDGCHC 3 cut(s) 41, 719, 758
SfaNI GCATC 6 cut(s) 636, 734, 933, 975, 1044, 1230
SfcI CTRYAG 2 cut(s) 475, 870
SinI GGWCC 3 cut(s) 244, 286, 946
SmiMI CAYNNNNRTG 4 cut(s) 749, 941, 995, 1143
SmlI CTYRAG 2 cut(s) 831, 1237
SmoI CTYRAG 2 cut(s) 831, 1237
SphI GCATGC 1 cut(s) 103
Sse9I AATT 2 cut(s) 465, 1172
SsiI CCGC 5 cut(s) 158, 168, 365, 534, 659
SspMI CTAG 4 cut(s) 765, 1028, 1178, 1218
StyD4I CCNGG 1 cut(s) 112
StyI CCWWGG 6 cut(s) 282, 596, 929, 990, 1027, 1177
TaaI ACNGT 2 cut(s) 695, 1201
TaiI ACGT 5 cut(s) 279, 377, 543, 753, 779
TaqI TCGA 1 cut(s) 981
TasI AATT 2 cut(s) 465, 1172
TauI GCSGC 2 cut(s) 368, 537
TfiI GAWTC 1 cut(s) 1167
Tru1I TTAA 2 cut(s) 690, 1085
Tru9I TTAA 2 cut(s) 690, 1085
TscAI CASTG 4 cut(s) 322, 580, 763, 850
TseFI GTSAC 1 cut(s) 59
TseI GCWGC 5 cut(s) 83, 194, 578, 869, 893
Tsp45I GTSAC 1 cut(s) 59
TspDTI ATGAA 5 cut(s) 163, 425, 536, 1237, 1406
TspGWI ACGGA 1 cut(s) 147
TspRI CASTG 4 cut(s) 322, 580, 763, 850
Van91I CCANNNNNTGG 2 cut(s) 498, 1015
VneI GTGCAC 1 cut(s) 754
VpaK11BI GGWCC 3 cut(s) 244, 286, 946
XagI CCTNNNNNAGG 1 cut(s) 870
XceI RCATGY 1 cut(s) 103
XcmI CCANNNNNNNNNTGG 1 cut(s) 280
XmaJI CCTAGG 2 cut(s) 1027, 1177
XmnI GAANNNNTTC 1 cut(s) 1383
XspI CTAG 4 cut(s) 765, 1028, 1178, 1218
Zsp2I ATGCAT 2 cut(s) 424, 1321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.