Rh6BG114700

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0016351
Physical Location & Seq
Reverse (-)
17859424 .. 17861508
2085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG114700.1

Sequence Viewer

Length: 1407 bp
ATGGAGCTACCCCCGGGCCTTGCATCCGATGAAGCTAGCCCCGAATGGTTTAGCAAGGCTGACAATGCGTGGCAGCTCACTGCTGCTACACTTGTAGGCATGCAGAGTGTGCCCGGCCTTATCATCTTGTATGGAAGCATAGTCAAGAAGAAGTGGGCAGTCAACTCTGCCTTCATGGCCATGTACGCCTTTGCAACCGTCTTGGTTTGTTGGGTGGGTTGGGGCTACCAAATGTCATTCGGTACCGAATTGTTCACCTTCCTGGGACGCCCCAACGTGGCCTTGGATGAGAAGTACCTATTTACAAAAGCATTCTCCGGGAAATTGCCGAACGCGACCATGATTTATTTTCAGTTTGTATTTGCAGCAATCACGTTGATTTTGATTGCGGGGGCGTTGTTGGGGAGGATGAATTTCCATGCGTGGATGTTGTTCGTACCACTTTGGGTGACATTTTCATATCCGATTACCGCTTATAGTATTTGGAATCCAAATGGATGGTTGGCAAAGATGGGTCTTATTGATTATTCTGGAGGGTATGTCATTCACCTTTCTTCAGGAGTTGCTGGTTTCACTGCTGCTTATTGGGTCGGACCTAGAGCAACTAGTGACAGGGAGAGATTCCCTCCAAACAACATCCTGCTTATGTTGGCAGGAGCTGGACTACTTTGGATGGGATGGACTGGATTCAATGGTGGAGATCCCTATAGTGTGAGCATGGACGCATCTCTAGCCGTCCTCAACACCCATGTATGTACTGCCTCTAGCTTGCTGACGTGGCTTGTCCTTGACATTATTTTCTTTGGGAAGCCCTCTGTGATTGGAGCCACACAGGGGATGATCACTGGCTTGGTTTGCATAACTCCTGCTGCGGGAGTGGTGCAAGGTTGGGCAGCAATCATAATGGGAATCATGTCCGGAAGTATCCCATGGTACACAATGATGGTCCTCCACAAGCAAAGCAAGCTCCTGAAGCATGTAGACGACACCATGGCCGTGTTCCACACCCACGCTGTGGCCGGAAGCTTGGGTGGTCTCCTTACCGGCTTCTTTTCCAATCCCAAGCTGTGTCGTCTTTTCTATCTAGTTGACAACTGGCAACACTACATCGGTCTTGCCTATGGCCTCCACGACGGTCGAGTTATGGCAGGGATGAAGCAAATGGGAATTCAGCTTCTGGGAATCGTGTTTGTGGTTGTTGTGAACGTTATCATAACCAGCTTAATCTGTTTGCTGTTGAGGCTCATCGTTCCACTTAGGATGACAGAAGATGACTTGAAAATAGGGGATGATGCAATTCACGGGGAGGAGGCATATGCATTGTGGGGAGATGGAGAGAAGTTTGATGGTTCTAAGCACAACTCAGTTTATGGCATGGATGACTTTGGTGTGGCAAGTAAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

468

Amino Acids

51.09

Weight (kDa)

6.4

Isoelectric Point (pI)

26.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 23 - 439 1.3e-82 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016351)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30820
malus_domestica MD11G1277400.v1.1
prunus_persica Prupe.8G233700_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0262941
rosa_laevigata RLG00000014406
rosa_multiflora Rmu_sc0000596.1_g000063 Rmu_sc0006493.1_g000013
rosa_roxburghii Rroxscaffold_177G00434070 Rroxscaffold_7G00204870
rosa_rugosa Rorug06G0001700
rosa_samantha Rh6AG121000 Rh6BG114700 Rh6CG113100 Rh6DG102400
rosa_wichuraiana Rw6G010390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 242
AccB1I GGYRCC 1 cut(s) 242
AccB7I CCANNNNNTGG 1 cut(s) 1015
AccI GTMKAC 1 cut(s) 981
AccII CGCG 1 cut(s) 335
AccIII TCCGGA 1 cut(s) 917
AciI CCGC 3 cut(s) 389, 471, 872
AclI AACGTT 1 cut(s) 1206
AclWI GGATC 1 cut(s) 695
AcoI YGGCCR 3 cut(s) 177, 993, 1017
AcsI RAATTY 2 cut(s) 412, 1167
AcuI CTGAAG 2 cut(s) 540, 992
AcyI GRCGYC 1 cut(s) 268
AdeI CACNNNGTG 1 cut(s) 1015
AfaI GTAC 6 cut(s) 185, 244, 296, 438, 757, 935
AfiI CCNNNNNNNGG 4 cut(s) 277, 834, 872, 1015
AgsI TTSAA 2 cut(s) 691, 1279
AhlI ACTAGT 1 cut(s) 605
AjiI CACGTC 1 cut(s) 777
AjnI CCWGG 1 cut(s) 261
Alw26I GTCTC 1 cut(s) 1040
AlwI GGATC 1 cut(s) 695
AlwNI CAGNNNCTG 2 cut(s) 659, 1177
Ama87I CYCGRG 1 cut(s) 13
Aor13HI TCCGGA 1 cut(s) 917
AoxI GGCC 7 cut(s) 16, 115, 177, 279, 993, 1017, 1123
ApeKI GCWGC 6 cut(s) 73, 83, 365, 578, 869, 893
ApoI RAATTY 2 cut(s) 412, 1167
Asp718I GGTACC 1 cut(s) 242
AspS9I GGNCC 3 cut(s) 16, 593, 946
AsuC2I CCSGG 4 cut(s) 14, 15, 114, 319
AsuHPI GGTGA 3 cut(s) 247, 460, 539
AsuNHI GCTAGC 1 cut(s) 35
AvaI CYCGRG 1 cut(s) 13
AvaII GGWCC 2 cut(s) 593, 946
BaeGI GKGCMC 1 cut(s) 114
BalI TGGCCA 1 cut(s) 179
BanI GGYRCC 1 cut(s) 242
BbvI GCAGC 6 cut(s) 70, 85, 377, 565, 856, 905
BccI CCATC 7 cut(s) 492, 505, 667, 672, 937, 1325, 1340
BceAI ACGGC 2 cut(s) 719, 980
BciT130I CCWGG 1 cut(s) 263
BciVI GTATCC 1 cut(s) 935
BclI TGATCA 1 cut(s) 840
BcnI CCSGG 4 cut(s) 14, 15, 114, 319
BcoDI GTCTC 1 cut(s) 1040
BcuI ACTAGT 1 cut(s) 605
BfaI CTAG 6 cut(s) 36, 597, 606, 731, 765, 1085
BfmI CTRYAG 1 cut(s) 706
BfuI GTATCC 1 cut(s) 935
BglI GCCNNNNNGGC 1 cut(s) 176
BisI GCNGC 6 cut(s) 74, 84, 366, 579, 870, 894
BlsI GCNGC 6 cut(s) 75, 85, 367, 580, 871, 895
Bme1390I CCNGG 5 cut(s) 14, 15, 114, 263, 319
Bme18I GGWCC 2 cut(s) 593, 946
BmeT110I CYCGRG 1 cut(s) 13
BmgBI CACGTC 1 cut(s) 777
BmgT120I GGNCC 3 cut(s) 16, 593, 946
BmiI GGNNCC 2 cut(s) 244, 826
BmrFI CCNGG 5 cut(s) 14, 15, 114, 263, 319
BmsI GCATC 3 cut(s) 32, 734, 1282
BmtI GCTAGC 1 cut(s) 39
BplI GAGNNNNNCTC 2 cut(s) 610, 642
BpmI CTGGAG 1 cut(s) 552
BpuMI CCSGG 4 cut(s) 14, 15, 114, 319
BsaHI GRCGYC 1 cut(s) 268
BsaI GGTCTC 1 cut(s) 1040
BsaJI CCNNGG 6 cut(s) 12, 13, 262, 282, 929, 990
BsaWI WCCGGW 1 cut(s) 917
BsaXI ACNNNNNCTCC 2 cut(s) 525, 555
Bsc4I CCNNNNNNNGG 4 cut(s) 277, 834, 872, 1015
Bse118I RCCGGY 1 cut(s) 1043
Bse1I ACTGG 3 cut(s) 688, 850, 1100
BseAI TCCGGA 1 cut(s) 917
BseBI CCWGG 1 cut(s) 263
BseDI CCNNGG 6 cut(s) 12, 13, 262, 282, 929, 990
BseLI CCNNNNNNNGG 4 cut(s) 277, 834, 872, 1015
BseMII CTCAG 1 cut(s) 1377
BseNI ACTGG 3 cut(s) 688, 850, 1100
BseRI GAGGAG 1 cut(s) 1322
BseSI GKGCMC 1 cut(s) 114
BseXI GCAGC 6 cut(s) 70, 85, 377, 565, 856, 905
Bsh1236I CGCG 1 cut(s) 335
Bsh1285I CGRYCG 1 cut(s) 1138
BshFI GGCC 7 cut(s) 18, 117, 179, 281, 995, 1019, 1125
BshNI GGYRCC 1 cut(s) 242
BsiEI CGRYCG 1 cut(s) 1138
BsiHKCI CYCGRG 1 cut(s) 13
BsiSI CCGG 6 cut(s) 14, 114, 318, 918, 1020, 1044
BslFI GGGAC 1 cut(s) 279
BslI CCNNNNNNNGG 4 cut(s) 277, 834, 872, 1015
BsmAI GTCTC 1 cut(s) 1040
BsmFI GGGAC 1 cut(s) 279
BsmI GAATGC 1 cut(s) 311
BsnI GGCC 7 cut(s) 18, 117, 179, 281, 995, 1019, 1125
Bso31I GGTCTC 1 cut(s) 1040
BsoBI CYCGRG 1 cut(s) 13
Bsp1286I GDGCHC 1 cut(s) 114
Bsp13I TCCGGA 1 cut(s) 917
Bsp143I GATC 2 cut(s) 700, 840
Bsp19I CCATGG 2 cut(s) 929, 990
BspACI CCGC 3 cut(s) 389, 471, 872
BspANI GGCC 7 cut(s) 18, 117, 179, 281, 995, 1019, 1125
BspCNI CTCAG 1 cut(s) 1376
BspEI TCCGGA 1 cut(s) 917
BspFNI CGCG 1 cut(s) 335
BspLI GGNNCC 2 cut(s) 244, 826
BspOI GCTAGC 1 cut(s) 39
BspPI GGATC 1 cut(s) 695
BspT107I GGYRCC 1 cut(s) 242
BspTNI GGTCTC 1 cut(s) 1040
BsrFI RCCGGY 1 cut(s) 1043
BsrI ACTGG 3 cut(s) 688, 850, 1100
BssAI RCCGGY 1 cut(s) 1043
BssECI CCNNGG 6 cut(s) 12, 13, 262, 282, 929, 990
BssMI GATC 2 cut(s) 700, 840
BssNI GRCGYC 1 cut(s) 268
BssT1I CCWWGG 3 cut(s) 282, 929, 990
Bst2UI CCWGG 1 cut(s) 263
Bst4CI ACNGT 2 cut(s) 199, 1136
BstACI GRCGYC 1 cut(s) 268
BstAPI GCANNNNNTGC 1 cut(s) 109
BstC8I GCNNGC 4 cut(s) 37, 101, 770, 965
BstDEI CTNAG 3 cut(s) 1256, 1353, 1363
BstDSI CCRYGG 2 cut(s) 929, 990
BstFNI CGCG 1 cut(s) 335
BstKTI GATC 2 cut(s) 703, 843
BstMAI GTCTC 1 cut(s) 1040
BstMBI GATC 2 cut(s) 700, 840
BstMCI CGRYCG 1 cut(s) 1138
BstNI CCWGG 1 cut(s) 263
BstNSI RCATGY 2 cut(s) 103, 980
BstSCI CCNGG 5 cut(s) 12, 13, 112, 261, 317
BstSFI CTRYAG 1 cut(s) 706
BstSLI GKGCMC 1 cut(s) 114
BstUI CGCG 1 cut(s) 335
BstV1I GCAGC 6 cut(s) 70, 85, 377, 565, 856, 905
BstX2I RGATCY 1 cut(s) 700
BstXI CCANNNNNNTGG 1 cut(s) 498
BstYI RGATCY 1 cut(s) 700
BsuI GTATCC 1 cut(s) 935
BsuRI GGCC 7 cut(s) 18, 117, 179, 281, 995, 1019, 1125
BtgI CCRYGG 2 cut(s) 929, 990
BtrI CACGTC 1 cut(s) 777
BtsI GCAGTG 2 cut(s) 78, 573
BtsIMutI CAGTG 3 cut(s) 78, 573, 843
Cac8I GCNNGC 4 cut(s) 37, 101, 770, 965
CaiI CAGNNNCTG 2 cut(s) 659, 1177
Cfr10I RCCGGY 1 cut(s) 1043
Cfr13I GGNCC 3 cut(s) 16, 593, 946
Cfr9I CCCGGG 1 cut(s) 13
CseI GACGC 2 cut(s) 276, 731
Csp6I GTAC 6 cut(s) 184, 243, 295, 437, 756, 934
CviQI GTAC 6 cut(s) 184, 243, 295, 437, 756, 934
DdeI CTNAG 3 cut(s) 1256, 1353, 1363
DpnI GATC 2 cut(s) 702, 842
DpnII GATC 2 cut(s) 700, 840
DraIII CACNNNGTG 1 cut(s) 1015
EaeI YGGCCR 3 cut(s) 177, 993, 1017
Eco130I CCWWGG 3 cut(s) 282, 929, 990
Eco31I GGTCTC 1 cut(s) 1040
Eco47I GGWCC 2 cut(s) 593, 946
Eco57I CTGAAG 2 cut(s) 540, 992
Eco88I CYCGRG 1 cut(s) 13
EcoRI GAATTC 1 cut(s) 1167
EcoRII CCWGG 1 cut(s) 261
EcoT14I CCWWGG 3 cut(s) 282, 929, 990
EcoT22I ATGCAT 1 cut(s) 1321
ErhI CCWWGG 3 cut(s) 282, 929, 990
FalI AAGNNNNNCTT 1 cut(s) 1387
FaqI GGGAC 1 cut(s) 279
FauI CCCGC 2 cut(s) 382, 865
FauNDI CATATG 1 cut(s) 1315
FbaI TGATCA 1 cut(s) 840
FblI GTMKAC 1 cut(s) 981
Fnu4HI GCNGC 6 cut(s) 74, 84, 366, 579, 870, 894
Fsp4HI GCNGC 6 cut(s) 74, 84, 366, 579, 870, 894
FspBI CTAG 6 cut(s) 36, 597, 606, 731, 765, 1085
GluI GCNGC 6 cut(s) 74, 84, 366, 579, 870, 894
GsuI CTGGAG 1 cut(s) 552
HaeIII GGCC 7 cut(s) 18, 117, 179, 281, 995, 1019, 1125
HapII CCGG 6 cut(s) 14, 114, 318, 918, 1020, 1044
HgaI GACGC 2 cut(s) 276, 731
Hin1I GRCGYC 1 cut(s) 268
HincII GTYRAC 2 cut(s) 163, 1090
HindII GTYRAC 2 cut(s) 163, 1090
HindIII AAGCTT 2 cut(s) 1024, 1401
HinfI GANTC 5 cut(s) 487, 621, 687, 909, 1182
HpaII CCGG 6 cut(s) 14, 114, 318, 918, 1020, 1044
HphI GGTGA 3 cut(s) 247, 460, 539
Hpy166II GTNNAC 6 cut(s) 163, 255, 936, 982, 1090, 1204
Hpy188I TCNGA 3 cut(s) 28, 465, 593
Hpy188III TCNNGA 5 cut(s) 145, 531, 558, 918, 970
Hpy8I GTNNAC 6 cut(s) 163, 255, 936, 982, 1090, 1204
Hpy99I CGWCG 1 cut(s) 1136
HpyAV CCTTC 2 cut(s) 181, 268
HpyCH4III ACNGT 2 cut(s) 199, 1136
HpyCH4IV ACGT 4 cut(s) 276, 374, 776, 1206
HpyCH4V TGCA 8 cut(s) 23, 103, 194, 365, 858, 883, 1295, 1319
HpyF3I CTNAG 3 cut(s) 1256, 1353, 1363
HpySE526I ACGT 4 cut(s) 276, 374, 776, 1206
Hsp92I GRCGYC 1 cut(s) 268
Kpn2I TCCGGA 1 cut(s) 917
KpnI GGTACC 1 cut(s) 246
Ksp22I TGATCA 1 cut(s) 840
Kzo9I GATC 2 cut(s) 700, 840
LmnI GCTCC 4 cut(s) 4, 656, 824, 972
Lsp1109I GCAGC 6 cut(s) 70, 85, 377, 565, 856, 905
LweI GCATC 3 cut(s) 32, 734, 1282
MaeI CTAG 6 cut(s) 36, 597, 606, 731, 765, 1085
MaeII ACGT 4 cut(s) 276, 374, 776, 1206
MaeIII GTNAC 2 cut(s) 448, 608
MalI GATC 2 cut(s) 702, 842
MboI GATC 2 cut(s) 700, 840
MboII GAAGA 3 cut(s) 160, 546, 1280
MflI RGATCY 1 cut(s) 700
MhlI GDGCHC 1 cut(s) 114
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 5 cut(s) 248, 323, 412, 1167, 1296
MluNI TGGCCA 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 571
Mox20I TGGCCA 1 cut(s) 179
Mph1103I ATGCAT 1 cut(s) 1321
MroI TCCGGA 1 cut(s) 917
MscI TGGCCA 1 cut(s) 179
MseI TTAA 2 cut(s) 1223, 1405
MslI CAYNNNNRTG 3 cut(s) 179, 941, 995
Msp20I TGGCCA 1 cut(s) 179
MspI CCGG 6 cut(s) 14, 114, 318, 918, 1020, 1044
MspR9I CCNGG 5 cut(s) 14, 15, 114, 263, 319
Mva1269I GAATGC 1 cut(s) 311
MvaI CCWGG 1 cut(s) 263
MvnI CGCG 1 cut(s) 335
NciI CCSGG 4 cut(s) 14, 15, 114, 319
NcoI CCATGG 2 cut(s) 929, 990
NdeI CATATG 1 cut(s) 1315
NdeII GATC 2 cut(s) 700, 840
NheI GCTAGC 1 cut(s) 35
NlaIV GGNNCC 2 cut(s) 244, 826
NmuCI GTSAC 2 cut(s) 448, 608
NsiI ATGCAT 1 cut(s) 1321
NspI RCATGY 2 cut(s) 103, 980
PaeI GCATGC 1 cut(s) 103
PctI GAATGC 1 cut(s) 311
PfeI GAWTC 5 cut(s) 487, 621, 687, 909, 1182
PflMI CCANNNNNTGG 1 cut(s) 1015
PfoI TCCNGGA 1 cut(s) 317
PkrI GCNGC 6 cut(s) 75, 85, 367, 580, 871, 895
Psp1406I AACGTT 1 cut(s) 1206
Psp6I CCWGG 1 cut(s) 261
PspGI CCWGG 1 cut(s) 261
PspN4I GGNNCC 2 cut(s) 244, 826
PspPI GGNCC 3 cut(s) 16, 593, 946
PstNI CAGNNNCTG 2 cut(s) 659, 1177
PsuI RGATCY 1 cut(s) 700
RsaI GTAC 6 cut(s) 185, 244, 296, 438, 757, 935
RsaNI GTAC 6 cut(s) 184, 243, 295, 437, 756, 934
RseI CAYNNNNRTG 3 cut(s) 179, 941, 995
SaqAI TTAA 2 cut(s) 1223, 1405
SatI GCNGC 6 cut(s) 74, 84, 366, 579, 870, 894
Sau3AI GATC 2 cut(s) 700, 840
Sau96I GGNCC 3 cut(s) 16, 593, 946
ScrFI CCNGG 5 cut(s) 14, 15, 114, 263, 319
SduI GDGCHC 1 cut(s) 114
SfaNI GCATC 3 cut(s) 32, 734, 1282
SfcI CTRYAG 1 cut(s) 706
SinI GGWCC 2 cut(s) 593, 946
SmaI CCCGGG 1 cut(s) 15
SmiMI CAYNNNNRTG 3 cut(s) 179, 941, 995
SpeI ACTAGT 1 cut(s) 605
SphI GCATGC 1 cut(s) 103
Sse9I AATT 5 cut(s) 248, 323, 412, 1167, 1296
SsiI CCGC 3 cut(s) 389, 471, 872
SspMI CTAG 6 cut(s) 36, 597, 606, 731, 765, 1085
StyD4I CCNGG 5 cut(s) 12, 13, 112, 261, 317
StyI CCWWGG 3 cut(s) 282, 929, 990
TaaI ACNGT 2 cut(s) 199, 1136
TaiI ACGT 4 cut(s) 279, 377, 779, 1209
TaqI TCGA 1 cut(s) 1138
TaqII GACCGA 1 cut(s) 1100
TasI AATT 5 cut(s) 248, 323, 412, 1167, 1296
TatI WGTACW 1 cut(s) 755
TfiI GAWTC 5 cut(s) 487, 621, 687, 909, 1182
Tru1I TTAA 2 cut(s) 1223, 1405
Tru9I TTAA 2 cut(s) 1223, 1405
TscAI CASTG 3 cut(s) 85, 580, 850
TseFI GTSAC 2 cut(s) 448, 608
TseI GCWGC 6 cut(s) 73, 83, 365, 578, 869, 893
Tsp45I GTSAC 2 cut(s) 448, 608
TspDTI ATGAA 5 cut(s) 45, 163, 425, 447, 1169
TspMI CCCGGG 1 cut(s) 13
TspRI CASTG 3 cut(s) 85, 580, 850
Van91I CCANNNNNTGG 1 cut(s) 1015
VpaK11BI GGWCC 2 cut(s) 593, 946
XapI RAATTY 2 cut(s) 412, 1167
XceI RCATGY 2 cut(s) 103, 980
XcmI CCANNNNNNNNNTGG 1 cut(s) 280
XmaI CCCGGG 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 981
XspI CTAG 6 cut(s) 36, 597, 606, 731, 765, 1085
Zsp2I ATGCAT 1 cut(s) 1321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.