Rw6G010390

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0016351
Physical Location & Seq
Reverse (-)
17994303 .. 17996446
2144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G010390.1

Sequence Viewer

Length: 1380 bp
ATGGAGCTACCCCCGGGTCTTGCATCCGACGAAGCTAGCCCCGAATGGTTTAGCAAGGCTGACAATGCGTGGCAGCTCACTGCTGCTACACTTGTAGGCATGCAGAGTGTGCCCGGCCTTATCATCTTGTATGGAAGCATAGTCAAGAAGAAGTGGGCAGTCAACTCGGCCTTCATGGCCATGTACGCCTTTGCAACCGTGTTGGTTTGTTGGGTGGGTTGGGGCTACCAAATGTCATTCGGTACTGAATTGTTCACCTTCCTGGGACGCCCCAACGTGGCCTTGGATGAGAAGTACCTATTTACAAAAGCATTCTCTGGGAAATTGCCGAACGCAACCATGATTTATTTTCAGTTTGTGTTTGCAGCAATCACGTTAATTTTGATTGCTGGGGCCTTGTTGGGGAGGATGAATTTCCATGCGTGGATGTTGTTCGTACCGCTTTGGGTGACATTTTCGTATCCAATAACCGCTTATAGTATTTGGAATCCAAATGGATGGTTGGCAAAGATGGGTCTTATTGATTATTCAGGAGGGTATGTCATTCACCTTTCTTCAGGAGTTGCGGGTTTCACTGCTGCTTATTGGGAGAGATTCCCTCCAAACAACATCCTGCTTATGTTGGCAGGAGCTGGACTACTTTGGATGGGATGGACTGGATTCAATGGTGGAGATCCCTACAGTGTGAGCATGGACGCATCTCTGGCTGTCCTCAACACCCATGTATGTACTGCCTCTAGCTTGCTGACGTGGCTCGTCCTTGACATTATTTTCTTTGGGAAGCCTTCTGTGATTGGAGCCACGCAGGGCATGATCACTGGTTTGGTTTGCATAACTCCTGCTGCGGGCGTGGTGCAAGGTTGGGCAGCAATCGTAATGGGAATCATGTCCGGAAGTATCCCATGGTACACAATGATGGTCCTCCACAAGCAAAGCAAGCTCCTGAAGCATGTAGACGACACCATGGCAGTGTTCCACACCCACGCTGTGGCAGGAAGCTTAGGTGGTCTCCTTACTGGCTTCTTTTCCAATCCTAAGCTATGTCGTCTTTTCTATCTAGTTGACAACTGGCAACACTACATCGGTCTTGCCTATGGCATCCACGACGGCCGATTTATGGATGGGATGAAACAAATGGGAATTCAACTTCTCGGAATTGTGTTTGTGGTTGTTGTGAACGTTATCATAACCAGCTTAATCTGTCTGTTGTTGAGGCTCATCGTTCCACTTCGGATGACAGAAGATGACTTGAAAATAGGGGATGATGCAATTCACGGCGAGGAGGCTTATGCATTGTGGGGAGATGGAGAGAAGTTTGATGGATCTAAGCACAACTCAGTTTATGGCATGGATGACTTTGGTGTGGCAAGTAAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

459

Amino Acids

50.23

Weight (kDa)

6.1

Isoelectric Point (pI)

27.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 23 - 430 2.9e-75 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016351)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30820
malus_domestica MD11G1277400.v1.1
prunus_persica Prupe.8G233700_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0262941
rosa_laevigata RLG00000014406
rosa_multiflora Rmu_sc0000596.1_g000063 Rmu_sc0006493.1_g000013
rosa_roxburghii Rroxscaffold_177G00434070 Rroxscaffold_7G00204870
rosa_rugosa Rorug06G0001700
rosa_samantha Rh6AG121000 Rh6BG114700 Rh6CG113100 Rh6DG102400
rosa_wichuraiana Rw6G010390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 988
AccI GTMKAC 1 cut(s) 954
AccIII TCCGGA 1 cut(s) 890
AciI CCGC 4 cut(s) 440, 471, 566, 845
AclI AACGTT 1 cut(s) 1179
AclWI GGATC 2 cut(s) 668, 1330
AcoI YGGCCR 2 cut(s) 177, 1108
AcsI RAATTY 2 cut(s) 412, 1140
AcuI CTGAAG 2 cut(s) 540, 965
AcyI GRCGYC 1 cut(s) 268
AdeI CACNNNGTG 1 cut(s) 988
AfaI GTAC 6 cut(s) 185, 244, 296, 438, 730, 908
AfiI CCNNNNNNNGG 5 cut(s) 277, 402, 845, 988, 1117
AgsI TTSAA 3 cut(s) 664, 1145, 1252
AjiI CACGTC 1 cut(s) 750
AjnI CCWGG 1 cut(s) 261
Alw26I GTCTC 1 cut(s) 1013
AlwI GGATC 2 cut(s) 668, 1330
AlwNI CAGNNNCTG 1 cut(s) 632
Ama87I CYCGRG 1 cut(s) 13
Aor13HI TCCGGA 1 cut(s) 890
AoxI GGCC 6 cut(s) 115, 168, 177, 279, 393, 1108
ApeKI GCWGC 6 cut(s) 73, 83, 365, 578, 842, 866
ApoI RAATTY 2 cut(s) 412, 1140
AspS9I GGNCC 2 cut(s) 393, 919
AsuC2I CCSGG 3 cut(s) 14, 15, 114
AsuHPI GGTGA 3 cut(s) 247, 460, 539
AsuNHI GCTAGC 1 cut(s) 35
AvaI CYCGRG 1 cut(s) 13
AvaII GGWCC 1 cut(s) 919
BaeGI GKGCMC 1 cut(s) 114
BalI TGGCCA 1 cut(s) 179
BbvI GCAGC 6 cut(s) 70, 85, 377, 565, 829, 878
BccI CCATC 8 cut(s) 492, 505, 640, 645, 910, 1115, 1298, 1313
BceAI ACGGC 2 cut(s) 1123, 1291
BcgI CGANNNNNNTGC 2 cut(s) 147, 181
BciT130I CCWGG 1 cut(s) 263
BciVI GTATCC 2 cut(s) 471, 908
BclI TGATCA 1 cut(s) 813
BcnI CCSGG 3 cut(s) 14, 15, 114
BcoDI GTCTC 1 cut(s) 1013
BfaI CTAG 3 cut(s) 36, 738, 1058
BfmI CTRYAG 1 cut(s) 679
BfuI GTATCC 2 cut(s) 471, 908
BglI GCCNNNNNGGC 1 cut(s) 176
BisI GCNGC 6 cut(s) 74, 84, 366, 579, 843, 867
BlsI GCNGC 6 cut(s) 75, 85, 367, 580, 844, 868
Bme1390I CCNGG 4 cut(s) 14, 15, 114, 263
Bme18I GGWCC 1 cut(s) 919
BmeT110I CYCGRG 1 cut(s) 13
BmgBI CACGTC 1 cut(s) 750
BmgT120I GGNCC 2 cut(s) 393, 919
BmiI GGNNCC 2 cut(s) 394, 799
BmrFI CCNGG 4 cut(s) 14, 15, 114, 263
BmsI GCATC 4 cut(s) 32, 707, 1107, 1255
BmtI GCTAGC 1 cut(s) 39
BplI GAGNNNNNCTC 2 cut(s) 583, 615
Bpu10I CCTNAGC 2 cut(s) 1000, 1035
BpuMI CCSGG 3 cut(s) 14, 15, 114
BsaHI GRCGYC 1 cut(s) 268
BsaI GGTCTC 1 cut(s) 1013
BsaJI CCNNGG 6 cut(s) 12, 13, 262, 282, 902, 963
BsaWI WCCGGW 1 cut(s) 890
BsaXI ACNNNNNCTCC 2 cut(s) 525, 555
Bsc4I CCNNNNNNNGG 5 cut(s) 277, 402, 845, 988, 1117
Bse1I ACTGG 4 cut(s) 661, 823, 1021, 1073
BseAI TCCGGA 1 cut(s) 890
BseBI CCWGG 1 cut(s) 263
BseDI CCNNGG 6 cut(s) 12, 13, 262, 282, 902, 963
BseLI CCNNNNNNNGG 5 cut(s) 277, 402, 845, 988, 1117
BseMII CTCAG 1 cut(s) 1350
BseNI ACTGG 4 cut(s) 661, 823, 1021, 1073
BseRI GAGGAG 1 cut(s) 1295
BseSI GKGCMC 1 cut(s) 114
BseX3I CGGCCG 1 cut(s) 1108
BseXI GCAGC 6 cut(s) 70, 85, 377, 565, 829, 878
BseYI CCCAGC 1 cut(s) 389
Bsh1285I CGRYCG 1 cut(s) 1111
BshFI GGCC 6 cut(s) 117, 170, 179, 281, 395, 1110
BsiEI CGRYCG 1 cut(s) 1111
BsiHKCI CYCGRG 1 cut(s) 13
BsiSI CCGG 3 cut(s) 14, 114, 891
BslFI GGGAC 1 cut(s) 279
BslI CCNNNNNNNGG 5 cut(s) 277, 402, 845, 988, 1117
BsmAI GTCTC 1 cut(s) 1013
BsmFI GGGAC 1 cut(s) 279
BsmI GAATGC 1 cut(s) 311
BsnI GGCC 6 cut(s) 117, 170, 179, 281, 395, 1110
Bso31I GGTCTC 1 cut(s) 1013
BsoBI CYCGRG 1 cut(s) 13
Bsp1286I GDGCHC 1 cut(s) 114
Bsp13I TCCGGA 1 cut(s) 890
Bsp143I GATC 3 cut(s) 673, 813, 1322
Bsp19I CCATGG 2 cut(s) 902, 963
BspACI CCGC 4 cut(s) 440, 471, 566, 845
BspANI GGCC 6 cut(s) 117, 170, 179, 281, 395, 1110
BspCNI CTCAG 1 cut(s) 1349
BspEI TCCGGA 1 cut(s) 890
BspLI GGNNCC 2 cut(s) 394, 799
BspOI GCTAGC 1 cut(s) 39
BspPI GGATC 2 cut(s) 668, 1330
BspTNI GGTCTC 1 cut(s) 1013
BsrI ACTGG 4 cut(s) 661, 823, 1021, 1073
BssECI CCNNGG 6 cut(s) 12, 13, 262, 282, 902, 963
BssMI GATC 3 cut(s) 673, 813, 1322
BssNI GRCGYC 1 cut(s) 268
BssT1I CCWWGG 3 cut(s) 282, 902, 963
Bst2UI CCWGG 1 cut(s) 263
Bst4CI ACNGT 2 cut(s) 199, 683
BstACI GRCGYC 1 cut(s) 268
BstAPI GCANNNNNTGC 1 cut(s) 109
BstC8I GCNNGC 5 cut(s) 37, 101, 743, 847, 938
BstDEI CTNAG 4 cut(s) 1000, 1035, 1326, 1336
BstDSI CCRYGG 2 cut(s) 902, 963
BstKTI GATC 3 cut(s) 676, 816, 1325
BstMAI GTCTC 1 cut(s) 1013
BstMBI GATC 3 cut(s) 673, 813, 1322
BstMCI CGRYCG 1 cut(s) 1111
BstMWI GCNNNNNNNGC 9 cut(s) 65, 109, 176, 185, 704, 751, 937, 946, 1373
BstNI CCWGG 1 cut(s) 263
BstNSI RCATGY 2 cut(s) 103, 953
BstSCI CCNGG 4 cut(s) 12, 13, 112, 261
BstSFI CTRYAG 1 cut(s) 679
BstSLI GKGCMC 1 cut(s) 114
BstV1I GCAGC 6 cut(s) 70, 85, 377, 565, 829, 878
BstX2I RGATCY 2 cut(s) 673, 1322
BstXI CCANNNNNNTGG 1 cut(s) 498
BstYI RGATCY 2 cut(s) 673, 1322
BstZI CGGCCG 1 cut(s) 1108
BsuI GTATCC 2 cut(s) 471, 908
BsuRI GGCC 6 cut(s) 117, 170, 179, 281, 395, 1110
BtgI CCRYGG 2 cut(s) 902, 963
BtrI CACGTC 1 cut(s) 750
BtsI GCAGTG 3 cut(s) 78, 573, 975
BtsIMutI CAGTG 5 cut(s) 78, 573, 688, 816, 975
Cac8I GCNNGC 5 cut(s) 37, 101, 743, 847, 938
CaiI CAGNNNCTG 1 cut(s) 632
Cfr13I GGNCC 2 cut(s) 393, 919
Cfr9I CCCGGG 1 cut(s) 13
CseI GACGC 2 cut(s) 276, 704
Csp6I GTAC 6 cut(s) 184, 243, 295, 437, 729, 907
CviQI GTAC 6 cut(s) 184, 243, 295, 437, 729, 907
DdeI CTNAG 4 cut(s) 1000, 1035, 1326, 1336
DpnI GATC 3 cut(s) 675, 815, 1324
DpnII GATC 3 cut(s) 673, 813, 1322
DraIII CACNNNGTG 1 cut(s) 988
EaeI YGGCCR 2 cut(s) 177, 1108
EagI CGGCCG 1 cut(s) 1108
EclXI CGGCCG 1 cut(s) 1108
Eco130I CCWWGG 3 cut(s) 282, 902, 963
Eco31I GGTCTC 1 cut(s) 1013
Eco47I GGWCC 1 cut(s) 919
Eco52I CGGCCG 1 cut(s) 1108
Eco57I CTGAAG 2 cut(s) 540, 965
Eco88I CYCGRG 1 cut(s) 13
EcoO109I RGGNCCY 1 cut(s) 393
EcoRI GAATTC 1 cut(s) 1140
EcoRII CCWGG 1 cut(s) 261
EcoT14I CCWWGG 3 cut(s) 282, 902, 963
EcoT22I ATGCAT 1 cut(s) 1294
ErhI CCWWGG 3 cut(s) 282, 902, 963
FalI AAGNNNNNCTT 1 cut(s) 1360
FaqI GGGAC 1 cut(s) 279
FauI CCCGC 2 cut(s) 559, 838
FbaI TGATCA 1 cut(s) 813
FblI GTMKAC 1 cut(s) 954
Fnu4HI GCNGC 6 cut(s) 74, 84, 366, 579, 843, 867
Fsp4HI GCNGC 6 cut(s) 74, 84, 366, 579, 843, 867
FspBI CTAG 3 cut(s) 36, 738, 1058
GluI GCNGC 6 cut(s) 74, 84, 366, 579, 843, 867
GsaI CCCAGC 1 cut(s) 393
HaeIII GGCC 6 cut(s) 117, 170, 179, 281, 395, 1110
HapII CCGG 3 cut(s) 14, 114, 891
HgaI GACGC 2 cut(s) 276, 704
Hin1I GRCGYC 1 cut(s) 268
HincII GTYRAC 2 cut(s) 163, 1063
HindII GTYRAC 2 cut(s) 163, 1063
HindIII AAGCTT 2 cut(s) 997, 1374
HinfI GANTC 4 cut(s) 487, 594, 660, 882
HpaII CCGG 3 cut(s) 14, 114, 891
HphI GGTGA 3 cut(s) 247, 460, 539
Hpy166II GTNNAC 6 cut(s) 163, 255, 909, 955, 1063, 1177
Hpy188I TCNGA 3 cut(s) 28, 1154, 1233
Hpy188III TCNNGA 5 cut(s) 145, 531, 558, 891, 943
Hpy8I GTNNAC 6 cut(s) 163, 255, 909, 955, 1063, 1177
Hpy99I CGWCG 2 cut(s) 32, 1109
HpyAV CCTTC 3 cut(s) 181, 268, 795
HpyCH4III ACNGT 2 cut(s) 199, 683
HpyCH4IV ACGT 4 cut(s) 276, 374, 749, 1179
HpyCH4V TGCA 8 cut(s) 23, 103, 194, 365, 831, 856, 1268, 1292
HpyF10VI GCNNNNNNNGC 9 cut(s) 65, 109, 176, 185, 704, 751, 937, 946, 1373
HpyF3I CTNAG 4 cut(s) 1000, 1035, 1326, 1336
HpySE526I ACGT 4 cut(s) 276, 374, 749, 1179
Hsp92I GRCGYC 1 cut(s) 268
Kpn2I TCCGGA 1 cut(s) 890
Ksp22I TGATCA 1 cut(s) 813
Kzo9I GATC 3 cut(s) 673, 813, 1322
LmnI GCTCC 4 cut(s) 4, 629, 797, 945
Lsp1109I GCAGC 6 cut(s) 70, 85, 377, 565, 829, 878
LweI GCATC 4 cut(s) 32, 707, 1107, 1255
MaeI CTAG 3 cut(s) 36, 738, 1058
MaeII ACGT 4 cut(s) 276, 374, 749, 1179
MaeIII GTNAC 1 cut(s) 448
MalI GATC 3 cut(s) 675, 815, 1324
MboI GATC 3 cut(s) 673, 813, 1322
MboII GAAGA 3 cut(s) 160, 546, 1253
MflI RGATCY 2 cut(s) 673, 1322
MhlI GDGCHC 1 cut(s) 114
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 7 cut(s) 248, 323, 378, 412, 1140, 1155, 1269
MluNI TGGCCA 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 51
MnlI CCTC 9 cut(s) 399, 527, 609, 722, 745, 932, 1206, 1273, 1276
Mox20I TGGCCA 1 cut(s) 179
Mph1103I ATGCAT 1 cut(s) 1294
MroI TCCGGA 1 cut(s) 890
MscI TGGCCA 1 cut(s) 179
MseI TTAA 3 cut(s) 377, 1196, 1378
MslI CAYNNNNRTG 3 cut(s) 179, 914, 968
Msp20I TGGCCA 1 cut(s) 179
MspI CCGG 3 cut(s) 14, 114, 891
MspR9I CCNGG 4 cut(s) 14, 15, 114, 263
Mva1269I GAATGC 1 cut(s) 311
MvaI CCWGG 1 cut(s) 263
MwoI GCNNNNNNNGC 9 cut(s) 65, 109, 176, 185, 704, 751, 937, 946, 1373
NciI CCSGG 3 cut(s) 14, 15, 114
NcoI CCATGG 2 cut(s) 902, 963
NdeII GATC 3 cut(s) 673, 813, 1322
NheI GCTAGC 1 cut(s) 35
NlaIV GGNNCC 2 cut(s) 394, 799
NmeAIII GCCGAG 1 cut(s) 146
NmuCI GTSAC 1 cut(s) 448
NsiI ATGCAT 1 cut(s) 1294
NspI RCATGY 2 cut(s) 103, 953
PaeI GCATGC 1 cut(s) 103
PctI GAATGC 1 cut(s) 311
PfeI GAWTC 4 cut(s) 487, 594, 660, 882
PflMI CCANNNNNTGG 1 cut(s) 988
PkrI GCNGC 6 cut(s) 75, 85, 367, 580, 844, 868
Psp1406I AACGTT 1 cut(s) 1179
Psp6I CCWGG 1 cut(s) 261
PspFI CCCAGC 1 cut(s) 389
PspGI CCWGG 1 cut(s) 261
PspN4I GGNNCC 2 cut(s) 394, 799
PspPI GGNCC 2 cut(s) 393, 919
PstNI CAGNNNCTG 1 cut(s) 632
PsuI RGATCY 2 cut(s) 673, 1322
RsaI GTAC 6 cut(s) 185, 244, 296, 438, 730, 908
RsaNI GTAC 6 cut(s) 184, 243, 295, 437, 729, 907
RseI CAYNNNNRTG 3 cut(s) 179, 914, 968
SaqAI TTAA 3 cut(s) 377, 1196, 1378
SatI GCNGC 6 cut(s) 74, 84, 366, 579, 843, 867
Sau3AI GATC 3 cut(s) 673, 813, 1322
Sau96I GGNCC 2 cut(s) 393, 919
ScrFI CCNGG 4 cut(s) 14, 15, 114, 263
SduI GDGCHC 1 cut(s) 114
SfaNI GCATC 4 cut(s) 32, 707, 1107, 1255
SfcI CTRYAG 1 cut(s) 679
SfiI GGCCNNNNNGGCC 1 cut(s) 176
SinI GGWCC 1 cut(s) 919
SmaI CCCGGG 1 cut(s) 15
SmiMI CAYNNNNRTG 3 cut(s) 179, 914, 968
SphI GCATGC 1 cut(s) 103
Sse9I AATT 7 cut(s) 248, 323, 378, 412, 1140, 1155, 1269
SsiI CCGC 4 cut(s) 440, 471, 566, 845
SspMI CTAG 3 cut(s) 36, 738, 1058
StyD4I CCNGG 4 cut(s) 12, 13, 112, 261
StyI CCWWGG 3 cut(s) 282, 902, 963
TaaI ACNGT 2 cut(s) 199, 683
TaiI ACGT 4 cut(s) 279, 377, 752, 1182
TaqII GACCGA 1 cut(s) 1073
TasI AATT 7 cut(s) 248, 323, 378, 412, 1140, 1155, 1269
TatI WGTACW 1 cut(s) 728
TfiI GAWTC 4 cut(s) 487, 594, 660, 882
Tru1I TTAA 3 cut(s) 377, 1196, 1378
Tru9I TTAA 3 cut(s) 377, 1196, 1378
TscAI CASTG 5 cut(s) 85, 580, 688, 823, 975
TseFI GTSAC 1 cut(s) 448
TseI GCWGC 6 cut(s) 73, 83, 365, 578, 842, 866
Tsp45I GTSAC 1 cut(s) 448
TspDTI ATGAA 3 cut(s) 163, 425, 1142
TspMI CCCGGG 1 cut(s) 13
TspRI CASTG 5 cut(s) 85, 580, 688, 823, 975
Van91I CCANNNNNTGG 1 cut(s) 988
VpaK11BI GGWCC 1 cut(s) 919
XapI RAATTY 2 cut(s) 412, 1140
XceI RCATGY 2 cut(s) 103, 953
XcmI CCANNNNNNNNNTGG 1 cut(s) 280
XmaI CCCGGG 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 954
XspI CTAG 3 cut(s) 36, 738, 1058
Zsp2I ATGCAT 1 cut(s) 1294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.