Rroxscaffold_7G00204870

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0016351
Physical Location & Seq
Forward (+)
54359352 .. 54361478
2127 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00204870.1

Sequence Viewer

Length: 1356 bp
ATGGAGCTACCCCCGGGCCTTGCATCCGACGAAGCTAGCCCCGAATGGTTTAGCAAGGCTGACAACGCGTGGCAGCTCACTGCTGCTACACTTGTAGGCATGCAGAGTGTGCCCGGCCTTATCATCTTGTATGGAAGCATAGTCAAAAAGAAGTGGGCAGTCAACTCGGCCTTCATGGCCATGTACGCCTTTGCAACCGTCTTGGTTTGTTGGGTGGGTTGGGGCTACCAAATGTCATTCGGTACCGAATTGTTCACCTTCCTGGGACGCCCCAACGTGGCCTTGGATGAGAAGTACCTATTTACAAAAGCATTCTCCGGGAAATTGCCGAACGCGACCATGATTTATTTTCAGTTTGTGTTTGCAGCAATCACGTTAATTTTGATTGCTGGGGCGTTGTTGGGGAGGATGAATTTCCATGCGTGGATGTTGTTCGTACCACTTTGGGTGACATTTTCGTATCCAATAACCGCTTATAGTATTTGGAATCCAAATGGATGGTTGGCAAAGATGGGTCTTATTGATTATTCTGGAGGGTCGGACCTAGAGCAACTAGTGACAGGGAGAGATTCCCCTCCAAACAACATCTTGCTTATGTTGGCAGGAGCTGGACTACTTTGGATGGGATGGACTGGATTCAATGGTGGAGATCCCTACAGTGTGAGCATGGACGCATCTCTGGCCGTCCTCAACACCCATGTATGTACTGCCTCTAGCTTGCTGACGTGGCTCGTCCTTGACATTATTTTCTTTGGGAAGCCTTCTGTGATTGGAGCCACACAGGGCATGATCACTGGCTTGGTTTGCATAACTCCTGCTGCGGGTGTGGTGCAAGGTTGGGCAGCAATTGTAATGGGAATCATGTCCGGAAGTATCCCATGGTACACAATGATGGTCCTCCACAAGCAAAGCAAGCTCCTGAAGCATGTAGACGACACCATGGCAGTGTTCCACACCCACGCTGTGGCAGGAAGCTTAGGTGGTCTCCTTACCGGCTTCTTTTCCAATCCCAAGCTATGTCGTCTTTTCTATCTAGTTGACAACTGGCAACACTACATCGGTCTTGCCTATGGCATCCACGACGGCCGATTTATGGATGGGATGAAACAAATGGGAATTCAACTTCTCGGAATTGTGTTTGTGGTTGTTGTGAACGTTATCATAACTAGCTTAATCTGTCTGCTGTTGAGGCTCATCGTTCCACTTAGGATGACAGAAGATGACTTGAAAATAGGGGATGATGCAATTCACGGGGAGGAGGCTTATGCATTGTGGGGAGATGGAGAGAAGTTTGATGGATCTAAGCACAACTCAGTTTATGGCATGGATGACTTTGGTGTGGCAAGTAAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

451

Amino Acids

49.28

Weight (kDa)

5.77

Isoelectric Point (pI)

28.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 23 - 180 2.6e-26 Ammonium Transporter Family
Ammonium_transp PF00909 191 - 422 4.2e-42 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016351)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30820
malus_domestica MD11G1277400.v1.1
prunus_persica Prupe.8G233700_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0262941
rosa_laevigata RLG00000014406
rosa_multiflora Rmu_sc0000596.1_g000063 Rmu_sc0006493.1_g000013
rosa_roxburghii Rroxscaffold_177G00434070 Rroxscaffold_7G00204870
rosa_rugosa Rorug06G0001700
rosa_samantha Rh6AG121000 Rh6BG114700 Rh6CG113100 Rh6DG102400
rosa_wichuraiana Rw6G010390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 242
AccB1I GGYRCC 1 cut(s) 242
AccB7I CCANNNNNTGG 1 cut(s) 964
AccI GTMKAC 1 cut(s) 930
AccII CGCG 2 cut(s) 68, 335
AccIII TCCGGA 1 cut(s) 866
AciI CCGC 2 cut(s) 471, 821
AclI AACGTT 1 cut(s) 1155
AclWI GGATC 2 cut(s) 644, 1306
AcoI YGGCCR 3 cut(s) 177, 681, 1084
AcsI RAATTY 2 cut(s) 412, 1116
AcuI CTGAAG 1 cut(s) 941
AcyI GRCGYC 1 cut(s) 268
AdeI CACNNNGTG 1 cut(s) 964
AfaI GTAC 6 cut(s) 185, 244, 296, 438, 706, 884
AfiI CCNNNNNNNGG 4 cut(s) 277, 821, 964, 1093
AflIII ACRYGT 1 cut(s) 66
AgsI TTSAA 3 cut(s) 640, 1121, 1228
AhlI ACTAGT 1 cut(s) 553
AjiI CACGTC 1 cut(s) 726
AjnI CCWGG 1 cut(s) 261
Alw26I GTCTC 1 cut(s) 989
AlwI GGATC 2 cut(s) 644, 1306
AlwNI CAGNNNCTG 1 cut(s) 608
Ama87I CYCGRG 1 cut(s) 13
Aor13HI TCCGGA 1 cut(s) 866
AoxI GGCC 7 cut(s) 16, 115, 168, 177, 279, 681, 1084
ApeKI GCWGC 5 cut(s) 73, 83, 365, 818, 842
ApoI RAATTY 2 cut(s) 412, 1116
Asp718I GGTACC 1 cut(s) 242
AspS9I GGNCC 3 cut(s) 16, 541, 895
AsuC2I CCSGG 4 cut(s) 14, 15, 114, 319
AsuHPI GGTGA 2 cut(s) 247, 460
AsuNHI GCTAGC 1 cut(s) 35
AvaI CYCGRG 1 cut(s) 13
AvaII GGWCC 2 cut(s) 541, 895
BaeGI GKGCMC 1 cut(s) 114
BalI TGGCCA 1 cut(s) 179
BanI GGYRCC 1 cut(s) 242
BbvI GCAGC 5 cut(s) 70, 85, 377, 805, 854
BccI CCATC 8 cut(s) 492, 505, 616, 621, 886, 1091, 1274, 1289
BceAI ACGGC 2 cut(s) 668, 1099
BcgI CGANNNNNNTGC 2 cut(s) 147, 181
BciT130I CCWGG 1 cut(s) 263
BciVI GTATCC 2 cut(s) 471, 884
BclI TGATCA 1 cut(s) 789
BcnI CCSGG 4 cut(s) 14, 15, 114, 319
BcoDI GTCTC 1 cut(s) 989
BcuI ACTAGT 1 cut(s) 553
BfaI CTAG 6 cut(s) 36, 545, 554, 714, 1034, 1167
BfmI CTRYAG 1 cut(s) 655
BfuI GTATCC 2 cut(s) 471, 884
BglI GCCNNNNNGGC 1 cut(s) 176
BisI GCNGC 5 cut(s) 74, 84, 366, 819, 843
BlsI GCNGC 5 cut(s) 75, 85, 367, 820, 844
Bme1390I CCNGG 5 cut(s) 14, 15, 114, 263, 319
Bme18I GGWCC 2 cut(s) 541, 895
BmeT110I CYCGRG 1 cut(s) 13
BmgBI CACGTC 1 cut(s) 726
BmgT120I GGNCC 3 cut(s) 16, 541, 895
BmiI GGNNCC 2 cut(s) 244, 775
BmrFI CCNGG 5 cut(s) 14, 15, 114, 263, 319
BmsI GCATC 4 cut(s) 32, 683, 1083, 1231
BmtI GCTAGC 1 cut(s) 39
BpmI CTGGAG 1 cut(s) 552
Bpu10I CCTNAGC 1 cut(s) 976
BpuMI CCSGG 4 cut(s) 14, 15, 114, 319
BsaHI GRCGYC 1 cut(s) 268
BsaI GGTCTC 1 cut(s) 989
BsaJI CCNNGG 6 cut(s) 12, 13, 262, 282, 878, 939
BsaWI WCCGGW 1 cut(s) 866
Bsc4I CCNNNNNNNGG 4 cut(s) 277, 821, 964, 1093
Bse118I RCCGGY 1 cut(s) 992
Bse1I ACTGG 3 cut(s) 637, 799, 1049
BseAI TCCGGA 1 cut(s) 866
BseBI CCWGG 1 cut(s) 263
BseDI CCNNGG 6 cut(s) 12, 13, 262, 282, 878, 939
BseLI CCNNNNNNNGG 4 cut(s) 277, 821, 964, 1093
BseMII CTCAG 1 cut(s) 1326
BseNI ACTGG 3 cut(s) 637, 799, 1049
BseRI GAGGAG 1 cut(s) 1271
BseSI GKGCMC 1 cut(s) 114
BseX3I CGGCCG 1 cut(s) 1084
BseXI GCAGC 5 cut(s) 70, 85, 377, 805, 854
BseYI CCCAGC 1 cut(s) 389
Bsh1236I CGCG 2 cut(s) 68, 335
Bsh1285I CGRYCG 1 cut(s) 1087
BshFI GGCC 7 cut(s) 18, 117, 170, 179, 281, 683, 1086
BshNI GGYRCC 1 cut(s) 242
BsiEI CGRYCG 1 cut(s) 1087
BsiHKCI CYCGRG 1 cut(s) 13
BsiSI CCGG 5 cut(s) 14, 114, 318, 867, 993
BslFI GGGAC 1 cut(s) 279
BslI CCNNNNNNNGG 4 cut(s) 277, 821, 964, 1093
BsmAI GTCTC 1 cut(s) 989
BsmFI GGGAC 1 cut(s) 279
BsmI GAATGC 1 cut(s) 311
BsnI GGCC 7 cut(s) 18, 117, 170, 179, 281, 683, 1086
Bso31I GGTCTC 1 cut(s) 989
BsoBI CYCGRG 1 cut(s) 13
Bsp1286I GDGCHC 1 cut(s) 114
Bsp13I TCCGGA 1 cut(s) 866
Bsp143I GATC 3 cut(s) 649, 789, 1298
Bsp19I CCATGG 2 cut(s) 878, 939
BspACI CCGC 2 cut(s) 471, 821
BspANI GGCC 7 cut(s) 18, 117, 170, 179, 281, 683, 1086
BspCNI CTCAG 1 cut(s) 1325
BspEI TCCGGA 1 cut(s) 866
BspFNI CGCG 2 cut(s) 68, 335
BspLI GGNNCC 2 cut(s) 244, 775
BspOI GCTAGC 1 cut(s) 39
BspPI GGATC 2 cut(s) 644, 1306
BspT107I GGYRCC 1 cut(s) 242
BspTNI GGTCTC 1 cut(s) 989
BsrFI RCCGGY 1 cut(s) 992
BsrI ACTGG 3 cut(s) 637, 799, 1049
BssAI RCCGGY 1 cut(s) 992
BssECI CCNNGG 6 cut(s) 12, 13, 262, 282, 878, 939
BssMI GATC 3 cut(s) 649, 789, 1298
BssNI GRCGYC 1 cut(s) 268
BssT1I CCWWGG 3 cut(s) 282, 878, 939
Bst2UI CCWGG 1 cut(s) 263
Bst4CI ACNGT 2 cut(s) 199, 659
BstACI GRCGYC 1 cut(s) 268
BstAPI GCANNNNNTGC 1 cut(s) 109
BstC8I GCNNGC 4 cut(s) 37, 101, 719, 914
BstDEI CTNAG 4 cut(s) 976, 1205, 1302, 1312
BstDSI CCRYGG 2 cut(s) 878, 939
BstFNI CGCG 2 cut(s) 68, 335
BstKTI GATC 3 cut(s) 652, 792, 1301
BstMAI GTCTC 1 cut(s) 989
BstMBI GATC 3 cut(s) 649, 789, 1298
BstMCI CGRYCG 1 cut(s) 1087
BstNI CCWGG 1 cut(s) 263
BstNSI RCATGY 2 cut(s) 103, 929
BstSCI CCNGG 5 cut(s) 12, 13, 112, 261, 317
BstSFI CTRYAG 1 cut(s) 655
BstSLI GKGCMC 1 cut(s) 114
BstUI CGCG 2 cut(s) 68, 335
BstV1I GCAGC 5 cut(s) 70, 85, 377, 805, 854
BstX2I RGATCY 2 cut(s) 649, 1298
BstXI CCANNNNNNTGG 1 cut(s) 498
BstYI RGATCY 2 cut(s) 649, 1298
BstZI CGGCCG 1 cut(s) 1084
BsuI GTATCC 2 cut(s) 471, 884
BsuRI GGCC 7 cut(s) 18, 117, 170, 179, 281, 683, 1086
BtgI CCRYGG 2 cut(s) 878, 939
BtrI CACGTC 1 cut(s) 726
BtsI GCAGTG 2 cut(s) 78, 951
BtsIMutI CAGTG 4 cut(s) 78, 664, 792, 951
Cac8I GCNNGC 4 cut(s) 37, 101, 719, 914
CaiI CAGNNNCTG 1 cut(s) 608
Cfr10I RCCGGY 1 cut(s) 992
Cfr13I GGNCC 3 cut(s) 16, 541, 895
Cfr9I CCCGGG 1 cut(s) 13
CseI GACGC 2 cut(s) 276, 680
Csp6I GTAC 6 cut(s) 184, 243, 295, 437, 705, 883
CviQI GTAC 6 cut(s) 184, 243, 295, 437, 705, 883
DdeI CTNAG 4 cut(s) 976, 1205, 1302, 1312
DpnI GATC 3 cut(s) 651, 791, 1300
DpnII GATC 3 cut(s) 649, 789, 1298
DraIII CACNNNGTG 1 cut(s) 964
EaeI YGGCCR 3 cut(s) 177, 681, 1084
EagI CGGCCG 1 cut(s) 1084
EclXI CGGCCG 1 cut(s) 1084
Eco130I CCWWGG 3 cut(s) 282, 878, 939
Eco31I GGTCTC 1 cut(s) 989
Eco47I GGWCC 2 cut(s) 541, 895
Eco52I CGGCCG 1 cut(s) 1084
Eco57I CTGAAG 1 cut(s) 941
Eco88I CYCGRG 1 cut(s) 13
EcoRI GAATTC 1 cut(s) 1116
EcoRII CCWGG 1 cut(s) 261
EcoT14I CCWWGG 3 cut(s) 282, 878, 939
EcoT22I ATGCAT 1 cut(s) 1270
ErhI CCWWGG 3 cut(s) 282, 878, 939
FalI AAGNNNNNCTT 1 cut(s) 1336
FaqI GGGAC 1 cut(s) 279
FauI CCCGC 1 cut(s) 814
FbaI TGATCA 1 cut(s) 789
FblI GTMKAC 1 cut(s) 930
Fnu4HI GCNGC 5 cut(s) 74, 84, 366, 819, 843
Fsp4HI GCNGC 5 cut(s) 74, 84, 366, 819, 843
FspBI CTAG 6 cut(s) 36, 545, 554, 714, 1034, 1167
GluI GCNGC 5 cut(s) 74, 84, 366, 819, 843
GsaI CCCAGC 1 cut(s) 393
GsuI CTGGAG 1 cut(s) 552
HaeIII GGCC 7 cut(s) 18, 117, 170, 179, 281, 683, 1086
HapII CCGG 5 cut(s) 14, 114, 318, 867, 993
HgaI GACGC 2 cut(s) 276, 680
Hin1I GRCGYC 1 cut(s) 268
HincII GTYRAC 2 cut(s) 163, 1039
HindII GTYRAC 2 cut(s) 163, 1039
HindIII AAGCTT 2 cut(s) 973, 1350
HinfI GANTC 4 cut(s) 487, 569, 636, 858
HpaII CCGG 5 cut(s) 14, 114, 318, 867, 993
HphI GGTGA 2 cut(s) 247, 460
Hpy166II GTNNAC 6 cut(s) 163, 255, 885, 931, 1039, 1153
Hpy188I TCNGA 3 cut(s) 28, 541, 1130
Hpy188III TCNNGA 3 cut(s) 531, 867, 919
Hpy8I GTNNAC 6 cut(s) 163, 255, 885, 931, 1039, 1153
Hpy99I CGWCG 2 cut(s) 32, 1085
HpyAV CCTTC 3 cut(s) 181, 268, 771
HpyCH4III ACNGT 2 cut(s) 199, 659
HpyCH4IV ACGT 4 cut(s) 276, 374, 725, 1155
HpyCH4V TGCA 8 cut(s) 23, 103, 194, 365, 807, 832, 1244, 1268
HpyF3I CTNAG 4 cut(s) 976, 1205, 1302, 1312
HpySE526I ACGT 4 cut(s) 276, 374, 725, 1155
Hsp92I GRCGYC 1 cut(s) 268
Kpn2I TCCGGA 1 cut(s) 866
KpnI GGTACC 1 cut(s) 246
Ksp22I TGATCA 1 cut(s) 789
Kzo9I GATC 3 cut(s) 649, 789, 1298
LmnI GCTCC 4 cut(s) 4, 605, 773, 921
Lsp1109I GCAGC 5 cut(s) 70, 85, 377, 805, 854
LweI GCATC 4 cut(s) 32, 683, 1083, 1231
MaeI CTAG 6 cut(s) 36, 545, 554, 714, 1034, 1167
MaeII ACGT 4 cut(s) 276, 374, 725, 1155
MaeIII GTNAC 2 cut(s) 448, 556
MalI GATC 3 cut(s) 651, 791, 1300
MboI GATC 3 cut(s) 649, 789, 1298
MboII GAAGA 1 cut(s) 1229
MfeI CAATTG 1 cut(s) 846
MflI RGATCY 2 cut(s) 649, 1298
MhlI GDGCHC 1 cut(s) 114
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 8 cut(s) 248, 323, 378, 412, 846, 1116, 1131, 1245
MluI ACGCGT 1 cut(s) 66
MluNI TGGCCA 1 cut(s) 179
MmeI TCCRAC 2 cut(s) 51, 519
MnlI CCTC 9 cut(s) 399, 527, 585, 698, 721, 908, 1182, 1249, 1252
Mox20I TGGCCA 1 cut(s) 179
Mph1103I ATGCAT 1 cut(s) 1270
MroI TCCGGA 1 cut(s) 866
MscI TGGCCA 1 cut(s) 179
MseI TTAA 3 cut(s) 377, 1172, 1354
MslI CAYNNNNRTG 3 cut(s) 179, 890, 944
Msp20I TGGCCA 1 cut(s) 179
MspI CCGG 5 cut(s) 14, 114, 318, 867, 993
MspR9I CCNGG 5 cut(s) 14, 15, 114, 263, 319
MunI CAATTG 1 cut(s) 846
Mva1269I GAATGC 1 cut(s) 311
MvaI CCWGG 1 cut(s) 263
MvnI CGCG 2 cut(s) 68, 335
NciI CCSGG 4 cut(s) 14, 15, 114, 319
NcoI CCATGG 2 cut(s) 878, 939
NdeII GATC 3 cut(s) 649, 789, 1298
NheI GCTAGC 1 cut(s) 35
NlaIV GGNNCC 2 cut(s) 244, 775
NmeAIII GCCGAG 1 cut(s) 146
NmuCI GTSAC 2 cut(s) 448, 556
NsiI ATGCAT 1 cut(s) 1270
NspI RCATGY 2 cut(s) 103, 929
PaeI GCATGC 1 cut(s) 103
PctI GAATGC 1 cut(s) 311
PfeI GAWTC 4 cut(s) 487, 569, 636, 858
PflMI CCANNNNNTGG 1 cut(s) 964
PfoI TCCNGGA 1 cut(s) 317
PkrI GCNGC 5 cut(s) 75, 85, 367, 820, 844
Psp1406I AACGTT 1 cut(s) 1155
Psp6I CCWGG 1 cut(s) 261
PspFI CCCAGC 1 cut(s) 389
PspGI CCWGG 1 cut(s) 261
PspN4I GGNNCC 2 cut(s) 244, 775
PspPI GGNCC 3 cut(s) 16, 541, 895
PstNI CAGNNNCTG 1 cut(s) 608
PsuI RGATCY 2 cut(s) 649, 1298
RsaI GTAC 6 cut(s) 185, 244, 296, 438, 706, 884
RsaNI GTAC 6 cut(s) 184, 243, 295, 437, 705, 883
RseI CAYNNNNRTG 3 cut(s) 179, 890, 944
SaqAI TTAA 3 cut(s) 377, 1172, 1354
SatI GCNGC 5 cut(s) 74, 84, 366, 819, 843
Sau3AI GATC 3 cut(s) 649, 789, 1298
Sau96I GGNCC 3 cut(s) 16, 541, 895
ScrFI CCNGG 5 cut(s) 14, 15, 114, 263, 319
SduI GDGCHC 1 cut(s) 114
SfaNI GCATC 4 cut(s) 32, 683, 1083, 1231
SfcI CTRYAG 1 cut(s) 655
SfiI GGCCNNNNNGGCC 1 cut(s) 176
SinI GGWCC 2 cut(s) 541, 895
SmaI CCCGGG 1 cut(s) 15
SmiMI CAYNNNNRTG 3 cut(s) 179, 890, 944
SpeI ACTAGT 1 cut(s) 553
SphI GCATGC 1 cut(s) 103
Sse9I AATT 8 cut(s) 248, 323, 378, 412, 846, 1116, 1131, 1245
SsiI CCGC 2 cut(s) 471, 821
SspMI CTAG 6 cut(s) 36, 545, 554, 714, 1034, 1167
StyD4I CCNGG 5 cut(s) 12, 13, 112, 261, 317
StyI CCWWGG 3 cut(s) 282, 878, 939
TaaI ACNGT 2 cut(s) 199, 659
TaiI ACGT 4 cut(s) 279, 377, 728, 1158
TaqII GACCGA 1 cut(s) 1049
TasI AATT 8 cut(s) 248, 323, 378, 412, 846, 1116, 1131, 1245
TatI WGTACW 1 cut(s) 704
TfiI GAWTC 4 cut(s) 487, 569, 636, 858
Tru1I TTAA 3 cut(s) 377, 1172, 1354
Tru9I TTAA 3 cut(s) 377, 1172, 1354
TscAI CASTG 4 cut(s) 85, 664, 799, 951
TseFI GTSAC 2 cut(s) 448, 556
TseI GCWGC 5 cut(s) 73, 83, 365, 818, 842
Tsp45I GTSAC 2 cut(s) 448, 556
TspDTI ATGAA 3 cut(s) 163, 425, 1118
TspMI CCCGGG 1 cut(s) 13
TspRI CASTG 4 cut(s) 85, 664, 799, 951
Van91I CCANNNNNTGG 1 cut(s) 964
VpaK11BI GGWCC 2 cut(s) 541, 895
XapI RAATTY 2 cut(s) 412, 1116
XceI RCATGY 2 cut(s) 103, 929
XcmI CCANNNNNNNNNTGG 1 cut(s) 280
XmaI CCCGGG 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 930
XspI CTAG 6 cut(s) 36, 545, 554, 714, 1034, 1167
Zsp2I ATGCAT 1 cut(s) 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.