Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0015436

Physical Location & Seq

Genomic Coordinates Forward (+)

88269 .. 89853

Gene Structure 1585 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1000200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 954 bp

ATGAACGACCTGATGACAAAGTCGTTCACCAACTACGTGGATCTAAAGAAAGAGGCTATGAAAGATTTGGACTTGGAAGCCGGGCATAATAACAATGTGGAGATGTCATCCTCCACCACAAATATGCACACCGACCTTGGTCTGTTCCTGGAAGAGGCCGAGAAGGTGAAGCAGGAGATGGCATCTGTTCGCGACATTCTCATGCGTCTGCAGCAAGCCAACGAGGAGAGCAAGTCCCTCCACAAGTCCGAGGCATTGCAATCCTTGCGCAGCAGAATCAATGCGGATATTACGGCAGTGCTGAAGAAAACAAAGACCATCAGATCTCAGCTAGAGGACATGGATCGTGCCAATGCCGCCAATAGGAGGCTCTCCGCCTACAAAGAAGGCACCCCGATTTACAGAACGAGGATGGCGGTGACGAGTGGCTTGCGCAAGAAGCTCAAGGAGCTTATGATGGATTTCCAGGGTCTGAGGCAGAGGATGATGAGCGAGTACAAGGAGACTGTTGGGAGACGCTACTTCACGGTGACGGGAGAGCAGGCGGATGAGGAGGTGATTGAAAAGATAATCTCGAATGGGGGTGGGAACGGGGCAGAGGAGTTTCTGGGGAAGGCAATACAAGAGCATGGGAGGGGGAAGGTGTTGGAAACGGTGGTGGAGATACAGGACAGGCACGACACGGCCAAGGACATCGAGAGGAGCCTGTTGGAGTTGCATCAGCTTTTCTTAGACATGGCAGTAATGGTGGAGGCTCAGGGGGAGCAGATGGATGATATAGAACACCATGTGATGAATGCTTCCCACTATGTCAAGGATGGAACAAAACATCTCAACACCGCCAAGGGCTACCAGAGGAGCAGCAGGAAGTGGATGTGTATCGGGTTGATAATTCTTCTGCTGCTCATTCTCGTCATCGTTATCCCCATTGCCACCAGCTTCTCTCACTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000149 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0006810 GO:0006887 GO:0006906 GO:0006996 GO:0008150 GO:0009504 GO:0009506 GO:0009524 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0016050 GO:0016192 GO:0022406 GO:0030054 GO:0031201 GO:0031224 GO:0032940 GO:0032991 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0046903 GO:0048278 GO:0048284 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0055044 GO:0061024 GO:0061025 GO:0071840 GO:0071944 GO:0090174 GO:0098796 GO:0140056

KEGG Pathways

Metabolic & Signaling

ko04130 ko04721 map04130 map04721

Pfam Domains

Protein Families

SNARE Syntaxin

Protein Analysis

318

Amino Acids

36.05

Weight (kDa)

6.44

Isoelectric Point (pI)

43.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Syntaxin	PF00804	45 - 196	8.8e-48	Syntaxin
SNARE	PF05739	255 - 307	1.3e-18	SNARE domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015436)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08560
fragaria_vesca	FvH4_7g04270
malus_domestica	MD12G1000200.v1.1
prunus_persica	Prupe.7G000700_v2.0.a1
pyrus_communis	pycom12g00030
rosa_chinensis	RchiOBHm_Chr1g0329221
rosa_laevigata	RLG00000029909
rosa_multiflora	Rmu_sc0000203.1_g000003 Rmu_sc0010272.1_g000006
rosa_roxburghii	Rroxscaffold_4G00321310
rosa_rugosa	Rorug01G0077200
rosa_samantha	Rh1AG096200 Rh1BG077000 Rh1CG093000 Rh1DG099500
rosa_wichuraiana	Rw1G007450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	269, 434
AccB1I	GGYRCC	1 cut(s)	389
AccII	CGCG	1 cut(s)	192
AciI	CCGC	6 cut(s)	284, 357, 375, 416, 545, 840
AclWI	GGATC	2 cut(s)	48, 351
AcoI	YGGCCR	1 cut(s)	684
AcuI	CTGAAG	1 cut(s)	323
AdeI	CACNNNGTG	1 cut(s)	790
AfaI	GTAC	1 cut(s)	497
AfiI	CCNNNNNNNGG	3 cut(s)	154, 363, 366
AgsI	TTSAA	1 cut(s)	563
AjnI	CCWGG	2 cut(s)	147, 465
AluBI	AGCT	5 cut(s)	331, 442, 451, 724, 939
AluI	AGCT	5 cut(s)	331, 442, 451, 724, 939
Alw26I	GTCTC	2 cut(s)	497, 508
AlwI	GGATC	2 cut(s)	48, 351
AlwNI	CAGNNNCTG	1 cut(s)	472
AoxI	GGCC	2 cut(s)	156, 684
ApeKI	GCWGC	4 cut(s)	211, 270, 861, 901
AspLEI	GCGC	2 cut(s)	270, 435
AsuC2I	CCSGG	1 cut(s)	82
AsuHPI	GGTGA	5 cut(s)	19, 178, 430, 541, 568
BanI	GGYRCC	1 cut(s)	389
BbvI	GCAGC	4 cut(s)	223, 282, 873, 888
BccI	CCATC	6 cut(s)	172, 326, 406, 451, 763, 812
BceAI	ACGGC	2 cut(s)	309, 699
BcgI	CGANNNNNNTGC	2 cut(s)	412, 446
BciT130I	CCWGG	2 cut(s)	149, 467
BcnI	CCSGG	1 cut(s)	82
BcoDI	GTCTC	2 cut(s)	497, 508
BfaI	CTAG	1 cut(s)	332
BfmI	CTRYAG	1 cut(s)	209
BglII	AGATCT	1 cut(s)	323
BisI	GCNGC	5 cut(s)	212, 271, 357, 862, 902
BlsI	GCNGC	5 cut(s)	213, 272, 358, 863, 903
Bme1390I	CCNGG	3 cut(s)	82, 149, 467
BmiI	GGNNCC	2 cut(s)	391, 704
BmrFI	CCNGG	3 cut(s)	82, 149, 467
BmsI	GCATC	2 cut(s)	191, 727
BoxI	GACNNNNGTC	1 cut(s)	138
Bpu10I	CCTNAGC	1 cut(s)	756
BpuEI	CTTGAG	1 cut(s)	428
BpuMI	CCSGG	1 cut(s)	82
BsaAI	YACGTR	1 cut(s)	37
BsaBI	GATNNNNATC	1 cut(s)	878
BsaJI	CCNNGG	5 cut(s)	136, 249, 466, 687, 843
BsaXI	ACNNNNNCTCC	2 cut(s)	218, 248
Bsc4I	CCNNNNNNNGG	3 cut(s)	154, 363, 366
Bse3DI	GCAATG	2 cut(s)	254, 927
Bse8I	GATNNNNATC	1 cut(s)	878
BseBI	CCWGG	2 cut(s)	149, 467
BseDI	CCNNGG	5 cut(s)	136, 249, 466, 687, 843
BseGI	GGATG	7 cut(s)	107, 417, 489, 553, 778, 823, 879
BseJI	GATNNNNATC	1 cut(s)	878
BseLI	CCNNNNNNNGG	3 cut(s)	154, 363, 366
BseMI	GCAATG	2 cut(s)	254, 927
BseMII	CTCAG	3 cut(s)	341, 464, 770
BseRI	GAGGAG	5 cut(s)	239, 566, 614, 715, 871
BseXI	GCAGC	4 cut(s)	223, 282, 873, 888
Bsh1236I	CGCG	1 cut(s)	192
BshFI	GGCC	2 cut(s)	158, 686
BshNI	GGYRCC	1 cut(s)	389
BsiSI	CCGG	1 cut(s)	81
BslFI	GGGAC	1 cut(s)	220
BslI	CCNNNNNNNGG	3 cut(s)	154, 363, 366
BsmAI	GTCTC	2 cut(s)	497, 508
BsmBI	CGTCTC	1 cut(s)	508
BsmFI	GGGAC	1 cut(s)	220
BsmI	GAATGC	1 cut(s)	802
BsnI	GGCC	2 cut(s)	158, 686
Bsp143I	GATC	3 cut(s)	40, 323, 343
Bsp68I	TCGCGA	1 cut(s)	192
BspACI	CCGC	6 cut(s)	284, 357, 375, 416, 545, 840
BspANI	GGCC	2 cut(s)	158, 686
BspCNI	CTCAG	3 cut(s)	340, 465, 769
BspFNI	CGCG	1 cut(s)	192
BspLI	GGNNCC	2 cut(s)	391, 704
BspMAI	CTGCAG	1 cut(s)	213
BspPI	GGATC	2 cut(s)	48, 351
BspT107I	GGYRCC	1 cut(s)	389
BsrDI	GCAATG	2 cut(s)	254, 927
BssECI	CCNNGG	5 cut(s)	136, 249, 466, 687, 843
BssMI	GATC	3 cut(s)	40, 323, 343
BssT1I	CCWWGG	3 cut(s)	136, 687, 843
Bst2UI	CCWGG	2 cut(s)	149, 467
Bst4CI	ACNGT	3 cut(s)	508, 529, 655
Bst6I	CTCTTC	1 cut(s)	147
BstAPI	GCANNNNNTGC	1 cut(s)	265
BstBAI	YACGTR	1 cut(s)	37
BstC8I	GCNNGC	3 cut(s)	216, 431, 543
BstDEI	CTNAG	4 cut(s)	327, 473, 730, 756
BstENI	CCTNNNNNAGG	1 cut(s)	152
BstF5I	GGATG	7 cut(s)	107, 417, 489, 553, 778, 823, 879
BstFNI	CGCG	1 cut(s)	192
BstHHI	GCGC	2 cut(s)	270, 435
BstKTI	GATC	3 cut(s)	43, 326, 346
BstMAI	GTCTC	2 cut(s)	497, 508
BstMBI	GATC	3 cut(s)	40, 323, 343
BstMWI	GCNNNNNNNGC	5 cut(s)	211, 265, 356, 439, 448
BstNI	CCWGG	2 cut(s)	149, 467
BstPAI	GACNNNNGTC	1 cut(s)	138
BstSCI	CCNGG	3 cut(s)	80, 147, 465
BstSFI	CTRYAG	1 cut(s)	209
BstUI	CGCG	1 cut(s)	192
BstV1I	GCAGC	4 cut(s)	223, 282, 873, 888
BstX2I	RGATCY	2 cut(s)	40, 323
BstXI	CCANNNNNNTGG	1 cut(s)	37
BstYI	RGATCY	2 cut(s)	40, 323
BsuRI	GGCC	2 cut(s)	158, 686
BtsCI	GGATG	7 cut(s)	107, 417, 489, 553, 778, 823, 879
BtsI	GCAGTG	1 cut(s)	303
BtsIMutI	CAGTG	1 cut(s)	303
BtuMI	TCGCGA	1 cut(s)	192
Cac8I	GCNNGC	3 cut(s)	216, 431, 543
CaiI	CAGNNNCTG	1 cut(s)	472
CfoI	GCGC	2 cut(s)	270, 435
CseI	GACGC	2 cut(s)	194, 525
Csp6I	GTAC	1 cut(s)	496
CviAII	CATG	5 cut(s)	202, 340, 629, 736, 788
CviQI	GTAC	1 cut(s)	496
DdeI	CTNAG	4 cut(s)	327, 473, 730, 756
DpnI	GATC	3 cut(s)	42, 325, 345
DpnII	GATC	3 cut(s)	40, 323, 343
DraIII	CACNNNGTG	1 cut(s)	790
EaeI	YGGCCR	1 cut(s)	684
Eam1104I	CTCTTC	1 cut(s)	147
EarI	CTCTTC	1 cut(s)	147
EciI	GGCGGA	2 cut(s)	364, 560
Eco130I	CCWWGG	3 cut(s)	136, 687, 843
Eco57I	CTGAAG	1 cut(s)	323
EcoNI	CCTNNNNNAGG	1 cut(s)	152
EcoRII	CCWGG	2 cut(s)	147, 465
EcoT14I	CCWWGG	3 cut(s)	136, 687, 843
ErhI	CCWWGG	3 cut(s)	136, 687, 843
Esp3I	CGTCTC	1 cut(s)	508
FaeI	CATG	5 cut(s)	205, 343, 632, 739, 791
FaqI	GGGAC	1 cut(s)	220
FatI	CATG	5 cut(s)	201, 339, 628, 735, 787
Fnu4HI	GCNGC	5 cut(s)	212, 271, 357, 862, 902
FokI	GGATG	7 cut(s)	94, 424, 496, 560, 785, 830, 886
Fsp4HI	GCNGC	5 cut(s)	212, 271, 357, 862, 902
FspBI	CTAG	1 cut(s)	332
FspI	TGCGCA	2 cut(s)	269, 434
GlaI	GCGC	2 cut(s)	269, 434
GluI	GCNGC	5 cut(s)	212, 271, 357, 862, 902
HaeIII	GGCC	2 cut(s)	158, 686
HapII	CCGG	1 cut(s)	81
HgaI	GACGC	2 cut(s)	194, 525
HhaI	GCGC	2 cut(s)	270, 435
Hin1II	CATG	5 cut(s)	205, 343, 632, 739, 791
Hin6I	GCGC	2 cut(s)	268, 433
HinP1I	GCGC	2 cut(s)	268, 433
HinfI	GANTC	1 cut(s)	276
HpaII	CCGG	1 cut(s)	81
HphI	GGTGA	5 cut(s)	19, 178, 430, 541, 568
Hpy166II	GTNNAC	1 cut(s)	27
Hpy188I	TCNGA	3 cut(s)	250, 323, 474
Hpy188III	TCNNGA	4 cut(s)	191, 574, 697, 951
Hpy8I	GTNNAC	1 cut(s)	27
HpyAV	CCTTC	4 cut(s)	157, 380, 607, 634
HpyCH4III	ACNGT	3 cut(s)	508, 529, 655
HpyCH4IV	ACGT	1 cut(s)	36
HpyCH4V	TGCA	4 cut(s)	127, 211, 259, 718
HpyF10VI	GCNNNNNNNGC	5 cut(s)	211, 265, 356, 439, 448
HpyF3I	CTNAG	4 cut(s)	327, 473, 730, 756
HpySE526I	ACGT	1 cut(s)	36
Hsp92II	CATG	5 cut(s)	205, 343, 632, 739, 791
HspAI	GCGC	2 cut(s)	268, 433
Kzo9I	GATC	3 cut(s)	40, 323, 343
LmnI	GCTCC	4 cut(s)	448, 702, 763, 858
Lsp1109I	GCAGC	4 cut(s)	223, 282, 873, 888
LweI	GCATC	2 cut(s)	191, 727
MaeI	CTAG	1 cut(s)	332
MaeII	ACGT	1 cut(s)	36
MaeIII	GTNAC	2 cut(s)	418, 529
MalI	GATC	3 cut(s)	42, 325, 345
MboI	GATC	3 cut(s)	40, 323, 343
MboII	GAAGA	3 cut(s)	164, 316, 887
MflI	RGATCY	2 cut(s)	40, 323
MluCI	AATT	1 cut(s)	891
MmeI	TCCRAC	2 cut(s)	627, 690
MslI	CAYNNNNRTG	2 cut(s)	122, 200
MspI	CCGG	1 cut(s)	81
MspR9I	CCNGG	3 cut(s)	82, 149, 467
Mva1269I	GAATGC	1 cut(s)	802
MvaI	CCWGG	2 cut(s)	149, 467
MvnI	CGCG	1 cut(s)	192
MwoI	GCNNNNNNNGC	5 cut(s)	211, 265, 356, 439, 448
NciI	CCSGG	1 cut(s)	82
NdeII	GATC	3 cut(s)	40, 323, 343
NlaIII	CATG	5 cut(s)	205, 343, 632, 739, 791
NlaIV	GGNNCC	2 cut(s)	391, 704
NmeAIII	GCCGAG	1 cut(s)	184
NmuCI	GTSAC	2 cut(s)	418, 529
NruI	TCGCGA	1 cut(s)	192
NsbI	TGCGCA	2 cut(s)	269, 434
PctI	GAATGC	1 cut(s)	802
PfeI	GAWTC	1 cut(s)	276
PflFI	GACNNNGTC	1 cut(s)	19
PfoI	TCCNGGA	1 cut(s)	147
PkrI	GCNGC	5 cut(s)	213, 272, 358, 863, 903
Ppu21I	YACGTR	1 cut(s)	37
PshAI	GACNNNNGTC	1 cut(s)	138
Psp6I	CCWGG	2 cut(s)	147, 465
PspGI	CCWGG	2 cut(s)	147, 465
PspN4I	GGNNCC	2 cut(s)	391, 704
PstI	CTGCAG	1 cut(s)	213
PstNI	CAGNNNCTG	1 cut(s)	472
PsuI	RGATCY	2 cut(s)	40, 323
PsyI	GACNNNGTC	1 cut(s)	19
RruI	TCGCGA	1 cut(s)	192
RsaI	GTAC	1 cut(s)	497
RsaNI	GTAC	1 cut(s)	496
RseI	CAYNNNNRTG	2 cut(s)	122, 200
SatI	GCNGC	5 cut(s)	212, 271, 357, 862, 902
Sau3AI	GATC	3 cut(s)	40, 323, 343
ScrFI	CCNGG	3 cut(s)	82, 149, 467
SfaNI	GCATC	2 cut(s)	191, 727
SfcI	CTRYAG	1 cut(s)	209
SmiMI	CAYNNNNRTG	2 cut(s)	122, 200
SmlI	CTYRAG	1 cut(s)	443
SmoI	CTYRAG	1 cut(s)	443
Sse9I	AATT	1 cut(s)	891
SsiI	CCGC	6 cut(s)	284, 357, 375, 416, 545, 840
SspMI	CTAG	1 cut(s)	332
StyD4I	CCNGG	3 cut(s)	80, 147, 465
StyI	CCWWGG	3 cut(s)	136, 687, 843
TaaI	ACNGT	3 cut(s)	508, 529, 655
TaiI	ACGT	1 cut(s)	39
TaqI	TCGA	2 cut(s)	575, 696
TasI	AATT	1 cut(s)	891
TatI	WGTACW	1 cut(s)	495
TauI	GCSGC	1 cut(s)	359
TfiI	GAWTC	1 cut(s)	276
TscAI	CASTG	1 cut(s)	303
TseFI	GTSAC	2 cut(s)	418, 529
TseI	GCWGC	4 cut(s)	211, 270, 861, 901
Tsp45I	GTSAC	2 cut(s)	418, 529
TspDTI	ATGAA	3 cut(s)	17, 74, 809
TspRI	CASTG	1 cut(s)	303
Tth111I	GACNNNGTC	1 cut(s)	19
XagI	CCTNNNNNAGG	1 cut(s)	152
XspI	CTAG	1 cut(s)	332

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.