MD12G1004100.v1.1

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0018758
Physical Location & Seq
Reverse (-)
589457 .. 590048
592 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1004100.v1.1.491

Sequence Viewer

Length: 414 bp
ATGAATTCTGATGGGGCTCTGCTCTGGTGTAGCGCAGGTGAGAACCGAAAGTCATCTTCACCGGAGAAGGAGGAAAGGTACTACACCACAAGAGGATCAGTGGGTGTGGATGGGAGTTGTAATGGCAACGGGAATGGGAAGGTTATGATGGACGGAAACAGTGTTGAGGACCGGGGGCTTGTTTGGCCGAAACTGTACGTCAGTTTGTCGAGCAAAGAGAAAGAAGAGGACTTTTTGGCTATAAGGTTGGTGAGTCCCGGTGCATGGCTCACTGATGTGTGCCAAGAGAGAGATGAAGTTAGGGAGAAGAAAAGTACCAAGAAGGTACTTGAACTGGAATTATTATATCATTTGCTGCATCATTTTTGTTTATTTTCGAGGGGTATGAGTACTCGTATTTTGATATTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

138

Amino Acids

15.42

Weight (kDa)

6.31

Isoelectric Point (pI)

28.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018758)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03880
malus_domestica MD12G1004100.v1.1 MD12G1004300.v1.1 MD14G1003000.v1.1
prunus_persica Prupe.7G004700_v2.0.a1 Prupe.7G004700_v2.0.a1 Prupe.7G004700_v2.0.a1
pyrus_communis pycom12g00370 pycom14g00090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 26
Acc36I ACCTGC 1 cut(s) 26
AclWI GGATC 1 cut(s) 103
AcoI YGGCCR 1 cut(s) 185
AcsI RAATTY 1 cut(s) 4
AfaI GTAC 5 cut(s) 80, 197, 316, 327, 391
AfiI CCNNNNNNNGG 1 cut(s) 264
AgsI TTSAA 1 cut(s) 332
AleI CACNNNNGTG 1 cut(s) 275
AlwI GGATC 1 cut(s) 103
AoxI GGCC 1 cut(s) 185
ApeKI GCWGC 1 cut(s) 355
ApoI RAATTY 1 cut(s) 4
AspLEI GCGC 1 cut(s) 35
AspS9I GGNCC 1 cut(s) 169
AsuC2I CCSGG 2 cut(s) 173, 258
AsuHPI GGTGA 3 cut(s) 50, 51, 262
AvaII GGWCC 1 cut(s) 169
BanII GRGCYC 1 cut(s) 19
BbvI GCAGC 1 cut(s) 342
BccI CCATC 3 cut(s) 5, 104, 142
BcnI CCSGG 2 cut(s) 173, 258
BfuAI ACCTGC 1 cut(s) 26
BisI GCNGC 1 cut(s) 356
BlsI GCNGC 1 cut(s) 357
BmcAI AGTACT 1 cut(s) 391
Bme1390I CCNGG 2 cut(s) 173, 258
Bme18I GGWCC 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 169
BmrFI CCNGG 2 cut(s) 173, 258
BmsI GCATC 1 cut(s) 367
BpuMI CCSGG 2 cut(s) 173, 258
BsaJI CCNNGG 1 cut(s) 172
BsaWI WCCGGW 1 cut(s) 61
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 1 cut(s) 264
Bse1I ACTGG 1 cut(s) 339
BseDI CCNNGG 1 cut(s) 172
BseGI GGATG 1 cut(s) 115
BseLI CCNNNNNNNGG 1 cut(s) 264
BseNI ACTGG 1 cut(s) 339
BseXI GCAGC 1 cut(s) 342
BshFI GGCC 1 cut(s) 187
BsiSI CCGG 3 cut(s) 62, 172, 258
BslFI GGGAC 1 cut(s) 240
BslI CCNNNNNNNGG 1 cut(s) 264
BsmFI GGGAC 1 cut(s) 240
BsnI GGCC 1 cut(s) 187
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 1 cut(s) 95
BspANI GGCC 1 cut(s) 187
BspMI ACCTGC 1 cut(s) 26
BspPI GGATC 1 cut(s) 103
BsrI ACTGG 1 cut(s) 339
BssECI CCNNGG 1 cut(s) 172
BssMI GATC 1 cut(s) 95
Bst4CI ACNGT 2 cut(s) 161, 195
Bst6I CTCTTC 1 cut(s) 219
BstF5I GGATG 1 cut(s) 115
BstHHI GCGC 1 cut(s) 35
BstKTI GATC 1 cut(s) 98
BstMBI GATC 1 cut(s) 95
BstMWI GCNNNNNNNGC 1 cut(s) 184
BstSCI CCNGG 2 cut(s) 171, 256
BstV1I GCAGC 1 cut(s) 342
BsuRI GGCC 1 cut(s) 187
BtsCI GGATG 1 cut(s) 115
BtsIMutI CAGTG 3 cut(s) 105, 166, 270
BveI ACCTGC 1 cut(s) 26
CfoI GCGC 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 169
Csp6I GTAC 5 cut(s) 79, 196, 315, 326, 390
CviAII CATG 1 cut(s) 264
CviJI RGCY 5 cut(s) 17, 178, 187, 239, 268
CviKI_1 RGCY 5 cut(s) 17, 178, 187, 239, 268
CviQI GTAC 5 cut(s) 79, 196, 315, 326, 390
DpnI GATC 1 cut(s) 97
DpnII GATC 1 cut(s) 95
EaeI YGGCCR 1 cut(s) 185
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 19
Eco47I GGWCC 1 cut(s) 169
EcoRI GAATTC 1 cut(s) 4
EcoT38I GRGCYC 1 cut(s) 19
FaeI CATG 1 cut(s) 267
FaiI YATR 5 cut(s) 146, 242, 265, 346, 386
FaqI GGGAC 1 cut(s) 240
FatI CATG 1 cut(s) 263
Fnu4HI GCNGC 1 cut(s) 356
FokI GGATG 1 cut(s) 122
FriOI GRGCYC 1 cut(s) 19
Fsp4HI GCNGC 1 cut(s) 356
GlaI GCGC 1 cut(s) 34
GluI GCNGC 1 cut(s) 356
HaeIII GGCC 1 cut(s) 187
HapII CCGG 3 cut(s) 62, 172, 258
HhaI GCGC 1 cut(s) 35
Hin1II CATG 1 cut(s) 267
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
HinfI GANTC 1 cut(s) 253
HpaII CCGG 3 cut(s) 62, 172, 258
HphI GGTGA 3 cut(s) 50, 51, 262
Hpy188I TCNGA 1 cut(s) 10
HpyAV CCTTC 3 cut(s) 61, 133, 316
HpyCH4III ACNGT 2 cut(s) 161, 195
HpyCH4IV ACGT 1 cut(s) 198
HpyCH4V TGCA 2 cut(s) 263, 358
HpyF10VI GCNNNNNNNGC 1 cut(s) 184
HpySE526I ACGT 1 cut(s) 198
Hsp92II CATG 1 cut(s) 267
HspAI GCGC 1 cut(s) 33
Kzo9I GATC 1 cut(s) 95
LpnPI CCDG 6 cut(s) 10, 21, 75, 185, 271, 320
Lsp1109I GCAGC 1 cut(s) 342
LweI GCATC 1 cut(s) 367
MaeII ACGT 1 cut(s) 198
MalI GATC 1 cut(s) 97
MboI GATC 1 cut(s) 95
MboII GAAGA 3 cut(s) 48, 236, 319
MhlI GDGCHC 1 cut(s) 19
MluCI AATT 2 cut(s) 4, 338
MlyI GAGTC 1 cut(s) 262
MnlI CCTC 5 cut(s) 64, 86, 160, 220, 372
MslI CAYNNNNRTG 1 cut(s) 275
MspI CCGG 3 cut(s) 62, 172, 258
MspR9I CCNGG 2 cut(s) 173, 258
MwoI GCNNNNNNNGC 1 cut(s) 184
NciI CCSGG 2 cut(s) 173, 258
NdeII GATC 1 cut(s) 95
NlaIII CATG 1 cut(s) 267
OliI CACNNNNGTG 1 cut(s) 275
PaqCI CACCTGC 1 cut(s) 26
PkrI GCNGC 1 cut(s) 357
PleI GAGTC 1 cut(s) 261
PpsI GAGTC 1 cut(s) 261
PspPI GGNCC 1 cut(s) 169
RsaI GTAC 5 cut(s) 80, 197, 316, 327, 391
RsaNI GTAC 5 cut(s) 79, 196, 315, 326, 390
RseI CAYNNNNRTG 1 cut(s) 275
SatI GCNGC 1 cut(s) 356
Sau3AI GATC 1 cut(s) 95
Sau96I GGNCC 1 cut(s) 169
ScaI AGTACT 1 cut(s) 391
SchI GAGTC 1 cut(s) 262
ScrFI CCNGG 2 cut(s) 173, 258
SduI GDGCHC 1 cut(s) 19
SetI ASST 6 cut(s) 40, 80, 144, 201, 248, 327
SfaNI GCATC 1 cut(s) 367
SinI GGWCC 1 cut(s) 169
SmiMI CAYNNNNRTG 1 cut(s) 275
Sse9I AATT 2 cut(s) 4, 338
StyD4I CCNGG 2 cut(s) 171, 256
TaaI ACNGT 2 cut(s) 161, 195
TaiI ACGT 1 cut(s) 201
TaqI TCGA 2 cut(s) 209, 377
TasI AATT 2 cut(s) 4, 338
TatI WGTACW 1 cut(s) 389
TscAI CASTG 3 cut(s) 105, 166, 277
TseI GCWGC 1 cut(s) 355
TspDTI ATGAA 2 cut(s) 17, 309
TspGWI ACGGA 1 cut(s) 168
TspRI CASTG 3 cut(s) 105, 166, 277
VpaK11BI GGWCC 1 cut(s) 169
XapI RAATTY 1 cut(s) 4
ZrmI AGTACT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.