MD14G1003000.v1.1

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0018758
Physical Location & Seq
Reverse (-)
399983 .. 402195
2213 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1003000.v1.1.491

Sequence Viewer

Length: 963 bp
ATGTTGAATCTAACGGTTACAGTAATCTTAAGTTTTTTTTATTGTCCGGCCGACGCAAAGGATAAAACGAGAAATGCAAGAGAGAGAAAGAGGAGGACGGAGAAACAACAGACAGGAAAGGAAGAAGAAGAAGAACAAGAAGAAATAATTCACAAAAACTTGAGAGGAGAAAAACAGAGAGCAACACCAGAACAACAGCAAAGACGGAGGAGGCGGAGAAGCGAGATTTACAGAAGAAGAATCTCCATCTCCATCTGGGCTTTCGTCGTCTTGGTGAAATTTGCTGGAAAATCCTCAAAGTTCTGTATGGAGACGGAGGAAGAGAGGAATAATCAGAGAGCTTCAGAACCTGAGTTTTTCCTGCAGTGGGGGAACAGGAAGAGGCTCAGATGTGTGAGAGTCAAAGGCTCGGAAATCTCAGCCGAGAGATTCAAATTCAATGGTGGCATGTGCAGGACAATCACATCGTCTCGGATTCATCGCTTTGCGGTCTCCACTTCCGGAAAAGACATCTCTGGTTTTCAATCCAATCGCCTTACCAGGAATTCTGACGGGAACCGAAAGTCATCTTCACCGGAGAAGGAGGAAAGGTACTACACCAGAAGAGGATCTGTGGGTGTGGGATCTGTGGGTGTGGATGAGAGTTGTAACGGGAATGGGAATGGGAAGGTTATGATGGACGGAAACAATGTTGAGGACCGGGGGCTTGTTTGGCCAAAGCTGTACATCACTTTGTCGAGCAAAGAGAAAGAAGAGGACTTTTTGGCCATGAAGGGTTGTAAACTTCCCCAGAGACCCAAGAAAAGGGCCAAACTCATCCAAAGAAGCTTACTTTTGGTGAGTCCCGGTGCATGGCTCACTGATATGTGCCAAGAGAGATATGAAGTTAGGGAGAAGAAAAGTACTAAGAAGAAATCAAGAGGATTGAAGGCTATGGGGAGCCTGGAAACCGACTCGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

321

Amino Acids

37.07

Weight (kDa)

10.03

Isoelectric Point (pI)

63.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 245 - 294 4.3e-22 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018758)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03880
malus_domestica MD12G1004100.v1.1 MD12G1004300.v1.1 MD14G1003000.v1.1
prunus_persica Prupe.7G004700_v2.0.a1 Prupe.7G004700_v2.0.a1 Prupe.7G004700_v2.0.a1
pyrus_communis pycom12g00370 pycom14g00090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 500
AciI CCGC 2 cut(s) 214, 488
AclWI GGATC 2 cut(s) 616, 631
AcoI YGGCCR 3 cut(s) 48, 713, 765
AcsI RAATTY 3 cut(s) 278, 434, 544
AcuI CTGAAG 1 cut(s) 327
AfaI GTAC 3 cut(s) 593, 725, 904
AfiI CCNNNNNNNGG 3 cut(s) 367, 804, 852
AflII CTTAAG 1 cut(s) 28
AgsI TTSAA 5 cut(s) 7, 433, 439, 524, 928
AjnI CCWGG 2 cut(s) 539, 942
AluBI AGCT 3 cut(s) 341, 721, 828
AluI AGCT 3 cut(s) 341, 721, 828
Alw26I GTCTC 4 cut(s) 305, 474, 496, 787
AlwI GGATC 2 cut(s) 616, 631
AlwNI CAGNNNCTG 1 cut(s) 350
Aor13HI TCCGGA 1 cut(s) 500
AoxI GGCC 4 cut(s) 48, 713, 765, 807
ApoI RAATTY 3 cut(s) 278, 434, 544
Asp700I GAANNNNTTC 1 cut(s) 147
AspS9I GGNCC 2 cut(s) 697, 807
AsuC2I CCSGG 2 cut(s) 701, 846
AsuHPI GGTGA 3 cut(s) 286, 564, 850
AvaII GGWCC 1 cut(s) 697
BalI TGGCCA 2 cut(s) 715, 767
BccI CCATC 3 cut(s) 254, 260, 670
BciT130I CCWGG 2 cut(s) 541, 944
BcnI CCSGG 2 cut(s) 701, 846
BcoDI GTCTC 4 cut(s) 305, 474, 496, 787
BfmI CTRYAG 1 cut(s) 362
BfrI CTTAAG 1 cut(s) 28
BmcAI AGTACT 1 cut(s) 904
Bme1390I CCNGG 4 cut(s) 541, 701, 846, 944
Bme18I GGWCC 1 cut(s) 697
BmgT120I GGNCC 2 cut(s) 697, 807
BmiI GGNNCC 2 cut(s) 557, 941
BmrFI CCNGG 4 cut(s) 541, 701, 846, 944
BpuEI CTTGAG 1 cut(s) 181
BpuMI CCSGG 2 cut(s) 701, 846
BsaI GGTCTC 2 cut(s) 496, 787
BsaJI CCNNGG 1 cut(s) 700
BsaWI WCCGGW 2 cut(s) 500, 574
BsaXI ACNNNNNCTCC 2 cut(s) 575, 605
Bsc4I CCNNNNNNNGG 3 cut(s) 367, 804, 852
BseAI TCCGGA 1 cut(s) 500
BseBI CCWGG 2 cut(s) 541, 944
BseDI CCNNGG 1 cut(s) 700
BseGI GGATG 2 cut(s) 643, 816
BseLI CCNNNNNNNGG 3 cut(s) 367, 804, 852
BseMII CTCAG 3 cut(s) 342, 400, 432
BseRI GAGGAG 3 cut(s) 106, 180, 223
BseX3I CGGCCG 1 cut(s) 48
BsgI GTGCAG 1 cut(s) 472
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 4 cut(s) 50, 715, 767, 809
BsiEI CGRYCG 1 cut(s) 51
BsiSI CCGG 5 cut(s) 47, 501, 575, 700, 846
BslFI GGGAC 1 cut(s) 828
BslI CCNNNNNNNGG 3 cut(s) 367, 804, 852
BsmAI GTCTC 4 cut(s) 305, 474, 496, 787
BsmBI CGTCTC 2 cut(s) 305, 474
BsmFI GGGAC 1 cut(s) 828
BsnI GGCC 4 cut(s) 50, 715, 767, 809
Bso31I GGTCTC 2 cut(s) 496, 787
Bsp13I TCCGGA 1 cut(s) 500
Bsp1407I TGTACA 1 cut(s) 723
Bsp143I GATC 2 cut(s) 608, 623
BspACI CCGC 2 cut(s) 214, 488
BspANI GGCC 4 cut(s) 50, 715, 767, 809
BspCNI CTCAG 3 cut(s) 343, 399, 431
BspEI TCCGGA 1 cut(s) 500
BspLI GGNNCC 2 cut(s) 557, 941
BspMAI CTGCAG 1 cut(s) 366
BspPI GGATC 2 cut(s) 616, 631
BspTI CTTAAG 1 cut(s) 28
BspTNI GGTCTC 2 cut(s) 496, 787
BsrGI TGTACA 1 cut(s) 723
BssECI CCNNGG 1 cut(s) 700
BssMI GATC 2 cut(s) 608, 623
Bst2UI CCWGG 2 cut(s) 541, 944
Bst4CI ACNGT 2 cut(s) 16, 22
Bst6I CTCTTC 4 cut(s) 315, 374, 598, 747
BstAFI CTTAAG 1 cut(s) 28
BstAUI TGTACA 1 cut(s) 723
BstDEI CTNAG 4 cut(s) 351, 386, 418, 906
BstF5I GGATG 2 cut(s) 643, 816
BstKTI GATC 2 cut(s) 611, 626
BstMAI GTCTC 4 cut(s) 305, 474, 496, 787
BstMBI GATC 2 cut(s) 608, 623
BstMCI CGRYCG 1 cut(s) 51
BstMWI GCNNNNNNNGC 1 cut(s) 712
BstNI CCWGG 2 cut(s) 541, 944
BstNSI RCATGY 1 cut(s) 451
BstSCI CCNGG 4 cut(s) 539, 699, 844, 942
BstSFI CTRYAG 1 cut(s) 362
BstX2I RGATCY 2 cut(s) 608, 623
BstYI RGATCY 2 cut(s) 608, 623
BstZI CGGCCG 1 cut(s) 48
BsuRI GGCC 4 cut(s) 50, 715, 767, 809
BtgZI GCGATG 1 cut(s) 464
BtsCI GGATG 2 cut(s) 643, 816
BtsI GCAGTG 1 cut(s) 371
BtsIMutI CAGTG 2 cut(s) 371, 858
CaiI CAGNNNCTG 1 cut(s) 350
Cfr13I GGNCC 2 cut(s) 697, 807
CseI GACGC 1 cut(s) 62
Csp6I GTAC 3 cut(s) 592, 724, 903
CviAII CATG 3 cut(s) 448, 769, 852
CviQI GTAC 3 cut(s) 592, 724, 903
DdeI CTNAG 4 cut(s) 351, 386, 418, 906
DpnI GATC 2 cut(s) 610, 625
DpnII GATC 2 cut(s) 608, 623
EaeI YGGCCR 3 cut(s) 48, 713, 765
EagI CGGCCG 1 cut(s) 48
Eam1104I CTCTTC 4 cut(s) 315, 374, 598, 747
EarI CTCTTC 4 cut(s) 315, 374, 598, 747
EciI GGCGGA 1 cut(s) 229
EclXI CGGCCG 1 cut(s) 48
Eco31I GGTCTC 2 cut(s) 496, 787
Eco47I GGWCC 1 cut(s) 697
Eco52I CGGCCG 1 cut(s) 48
Eco57I CTGAAG 1 cut(s) 327
EcoRI GAATTC 1 cut(s) 544
EcoRII CCWGG 2 cut(s) 539, 942
Esp3I CGTCTC 2 cut(s) 305, 474
FaeI CATG 3 cut(s) 451, 772, 855
FaiI YATR 8 cut(s) 308, 449, 674, 770, 853, 866, 882, 935
FaqI GGGAC 1 cut(s) 828
FatI CATG 3 cut(s) 447, 768, 851
FokI GGATG 2 cut(s) 650, 803
HaeIII GGCC 4 cut(s) 50, 715, 767, 809
HapII CCGG 5 cut(s) 47, 501, 575, 700, 846
HgaI GACGC 1 cut(s) 62
Hin1II CATG 3 cut(s) 451, 772, 855
HindIII AAGCTT 1 cut(s) 826
HinfI GANTC 7 cut(s) 7, 240, 399, 429, 475, 841, 953
HpaII CCGG 5 cut(s) 47, 501, 575, 700, 846
HphI GGTGA 3 cut(s) 286, 564, 850
Hpy166II GTNNAC 1 cut(s) 782
Hpy188I TCNGA 7 cut(s) 336, 346, 389, 412, 474, 550, 958
Hpy188III TCNNGA 2 cut(s) 501, 918
Hpy8I GTNNAC 1 cut(s) 782
Hpy99I CGWCG 2 cut(s) 56, 269
HpyAV CCTTC 4 cut(s) 574, 661, 766, 922
HpyCH4III ACNGT 2 cut(s) 16, 22
HpyCH4V TGCA 4 cut(s) 77, 364, 453, 851
HpyF10VI GCNNNNNNNGC 1 cut(s) 712
HpyF3I CTNAG 4 cut(s) 351, 386, 418, 906
Hsp92II CATG 3 cut(s) 451, 772, 855
Kpn2I TCCGGA 1 cut(s) 500
Kzo9I GATC 2 cut(s) 608, 623
LmnI GCTCC 1 cut(s) 939
MaeIII GTNAC 2 cut(s) 16, 647
MalI GATC 2 cut(s) 610, 625
MboI GATC 2 cut(s) 608, 623
MflI RGATCY 2 cut(s) 608, 623
MlsI TGGCCA 2 cut(s) 715, 767
MluCI AATT 4 cut(s) 147, 278, 434, 544
MluNI TGGCCA 2 cut(s) 715, 767
MlyI GAGTC 3 cut(s) 408, 850, 947
Mox20I TGGCCA 2 cut(s) 715, 767
MroI TCCGGA 1 cut(s) 500
MroXI GAANNNNTTC 1 cut(s) 147
MscI TGGCCA 2 cut(s) 715, 767
MseI TTAA 1 cut(s) 29
MslI CAYNNNNRTG 1 cut(s) 863
Msp20I TGGCCA 2 cut(s) 715, 767
MspCI CTTAAG 1 cut(s) 28
MspI CCGG 5 cut(s) 47, 501, 575, 700, 846
MspR9I CCNGG 4 cut(s) 541, 701, 846, 944
MvaI CCWGG 2 cut(s) 541, 944
MwoI GCNNNNNNNGC 1 cut(s) 712
NciI CCSGG 2 cut(s) 701, 846
NdeII GATC 2 cut(s) 608, 623
NlaIII CATG 3 cut(s) 451, 772, 855
NlaIV GGNNCC 2 cut(s) 557, 941
NmeAIII GCCGAG 1 cut(s) 448
NspI RCATGY 1 cut(s) 451
PdmI GAANNNNTTC 1 cut(s) 147
PfeI GAWTC 4 cut(s) 7, 240, 429, 475
PleI GAGTC 3 cut(s) 407, 849, 947
PpsI GAGTC 3 cut(s) 407, 849, 947
Psp6I CCWGG 2 cut(s) 539, 942
PspGI CCWGG 2 cut(s) 539, 942
PspN4I GGNNCC 2 cut(s) 557, 941
PspPI GGNCC 2 cut(s) 697, 807
PstI CTGCAG 1 cut(s) 366
PstNI CAGNNNCTG 1 cut(s) 350
PsuI RGATCY 2 cut(s) 608, 623
RsaI GTAC 3 cut(s) 593, 725, 904
RsaNI GTAC 3 cut(s) 592, 724, 903
RseI CAYNNNNRTG 1 cut(s) 863
SaqAI TTAA 1 cut(s) 29
Sau3AI GATC 2 cut(s) 608, 623
Sau96I GGNCC 2 cut(s) 697, 807
ScaI AGTACT 1 cut(s) 904
SchI GAGTC 3 cut(s) 408, 850, 947
ScrFI CCNGG 4 cut(s) 541, 701, 846, 944
SetI ASST 6 cut(s) 343, 352, 593, 672, 723, 830
SfcI CTRYAG 1 cut(s) 362
SinI GGWCC 1 cut(s) 697
SmiMI CAYNNNNRTG 1 cut(s) 863
SmlI CTYRAG 2 cut(s) 28, 160
SmoI CTYRAG 2 cut(s) 28, 160
Sse9I AATT 4 cut(s) 147, 278, 434, 544
SsiI CCGC 2 cut(s) 214, 488
StyD4I CCNGG 4 cut(s) 539, 699, 844, 942
TaaI ACNGT 2 cut(s) 16, 22
TaqI TCGA 1 cut(s) 737
TasI AATT 4 cut(s) 147, 278, 434, 544
TatI WGTACW 2 cut(s) 723, 902
TfiI GAWTC 4 cut(s) 7, 240, 429, 475
Tru1I TTAA 1 cut(s) 29
Tru9I TTAA 1 cut(s) 29
TscAI CASTG 2 cut(s) 371, 865
TspDTI ATGAA 3 cut(s) 467, 785, 897
TspGWI ACGGA 4 cut(s) 113, 220, 329, 696
TspRI CASTG 2 cut(s) 371, 865
Vha464I CTTAAG 1 cut(s) 28
VpaK11BI GGWCC 1 cut(s) 697
XapI RAATTY 3 cut(s) 278, 434, 544
XceI RCATGY 1 cut(s) 451
XmnI GAANNNNTTC 1 cut(s) 147
ZrmI AGTACT 1 cut(s) 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.