MD12G1004300.v1.1

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0018758
Physical Location & Seq
Reverse (-)
595892 .. 597961
2070 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1004300.v1.1.491

Sequence Viewer

Length: 690 bp
ATGGAAACATCTCATCGTATGGAGACGGAAGAAGAGAGGAATAATAAGAGAGCTTCAGAACCAGAGTTTTTCTTGCAGTGGGGGAACAGAAAGAGACTCAGATGTGTGAGAGTCAAAGGCACAGAAATCTCAGCCGACAGATTCAAATTCAATGGTGGCATGTGCAGGAGAATCACATCGTCTCGGATTCATCGCTTTGCGGTCTCCACTTCCGGAAAAAATGACATCTCTGGATTTCAATCCAATCGCCTCACCAGGAATTCTGATGGGGGTCTGCTCTGGTGTAGCGCAGGTGAGAACCGAAAGTCATCTTCACCGGAGAAGGAGGAAAGGTACTACACCACAAGGGGATCAGTGGGTGTGGATGAGAGTTGTAATGGCAACGGGAATGGGAAGGTTATGATGGATGGAAACAGTGTTGAGGACCGGGGGCTTGTTTGGCCGAAACTGTACATCAGTTTGTCGAGCAAAGAGAAAGAAGAGGACTTTTTGGCCATGAAGGGTTGTAAACTTCCCCAAAGACCCAAAAAAAGGGCCAAACTCATCCAAAGAAGCTTACTTTTGGTGAGTCCCGGTGCATGGCTCACTGATATGTGCCAAGAGAGATATGAAGTTAGGGAGAAGAAGAGTACCAAGAAGAGACCAAGAGGATTGAAGGGTATGGGGAGCCTGGAAACCGAGTCGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

230

Amino Acids

26.14

Weight (kDa)

9.74

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 154 - 203 2.5e-22 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018758)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03880
malus_domestica MD12G1004100.v1.1 MD12G1004300.v1.1 MD14G1003000.v1.1
prunus_persica Prupe.7G004700_v2.0.a1 Prupe.7G004700_v2.0.a1 Prupe.7G004700_v2.0.a1
pyrus_communis pycom12g00370 pycom14g00090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 281
Acc36I ACCTGC 1 cut(s) 281
AccIII TCCGGA 1 cut(s) 212
AciI CCGC 1 cut(s) 200
AclWI GGATC 1 cut(s) 358
AcoI YGGCCR 2 cut(s) 440, 492
AcsI RAATTY 2 cut(s) 146, 259
AcuI CTGAAG 1 cut(s) 39
AfaI GTAC 3 cut(s) 335, 452, 631
AfiI CCNNNNNNNGG 2 cut(s) 531, 579
AgsI TTSAA 4 cut(s) 145, 151, 239, 655
AjnI CCWGG 2 cut(s) 254, 669
AluBI AGCT 2 cut(s) 53, 555
AluI AGCT 2 cut(s) 53, 555
Alw26I GTCTC 5 cut(s) 17, 88, 186, 208, 634
AlwI GGATC 1 cut(s) 358
Aor13HI TCCGGA 1 cut(s) 212
AoxI GGCC 3 cut(s) 440, 492, 534
ApoI RAATTY 2 cut(s) 146, 259
AspLEI GCGC 1 cut(s) 290
AspS9I GGNCC 2 cut(s) 424, 534
AsuC2I CCSGG 2 cut(s) 428, 573
AsuHPI GGTGA 4 cut(s) 244, 305, 306, 577
AvaII GGWCC 1 cut(s) 424
BalI TGGCCA 1 cut(s) 494
BccI CCATC 3 cut(s) 260, 397, 401
BciT130I CCWGG 2 cut(s) 256, 671
BcnI CCSGG 2 cut(s) 428, 573
BcoDI GTCTC 5 cut(s) 17, 88, 186, 208, 634
BfuAI ACCTGC 1 cut(s) 281
Bme1390I CCNGG 4 cut(s) 256, 428, 573, 671
Bme18I GGWCC 1 cut(s) 424
BmgT120I GGNCC 2 cut(s) 424, 534
BmiI GGNNCC 1 cut(s) 668
BmrFI CCNGG 4 cut(s) 256, 428, 573, 671
BpuMI CCSGG 2 cut(s) 428, 573
BsaBI GATNNNNATC 1 cut(s) 238
BsaI GGTCTC 2 cut(s) 208, 634
BsaJI CCNNGG 1 cut(s) 427
BsaWI WCCGGW 2 cut(s) 212, 316
BsaXI ACNNNNNCTCC 2 cut(s) 317, 347
Bsc4I CCNNNNNNNGG 2 cut(s) 531, 579
Bse8I GATNNNNATC 1 cut(s) 238
BseAI TCCGGA 1 cut(s) 212
BseBI CCWGG 2 cut(s) 256, 671
BseDI CCNNGG 1 cut(s) 427
BseGI GGATG 3 cut(s) 370, 412, 543
BseJI GATNNNNATC 1 cut(s) 238
BseLI CCNNNNNNNGG 2 cut(s) 531, 579
BseMII CTCAG 2 cut(s) 112, 144
BsgI GTGCAG 1 cut(s) 184
BshFI GGCC 3 cut(s) 442, 494, 536
BsiSI CCGG 4 cut(s) 213, 317, 427, 573
BslFI GGGAC 1 cut(s) 555
BslI CCNNNNNNNGG 2 cut(s) 531, 579
BsmAI GTCTC 5 cut(s) 17, 88, 186, 208, 634
BsmBI CGTCTC 2 cut(s) 17, 186
BsmFI GGGAC 1 cut(s) 555
BsnI GGCC 3 cut(s) 442, 494, 536
Bso31I GGTCTC 2 cut(s) 208, 634
Bsp13I TCCGGA 1 cut(s) 212
Bsp1407I TGTACA 1 cut(s) 450
Bsp143I GATC 1 cut(s) 350
BspACI CCGC 1 cut(s) 200
BspANI GGCC 3 cut(s) 442, 494, 536
BspCNI CTCAG 2 cut(s) 111, 143
BspEI TCCGGA 1 cut(s) 212
BspLI GGNNCC 1 cut(s) 668
BspMI ACCTGC 1 cut(s) 281
BspPI GGATC 1 cut(s) 358
BspTNI GGTCTC 2 cut(s) 208, 634
BsrGI TGTACA 1 cut(s) 450
BssECI CCNNGG 1 cut(s) 427
BssMI GATC 1 cut(s) 350
Bst2UI CCWGG 2 cut(s) 256, 671
Bst4CI ACNGT 2 cut(s) 416, 450
Bst6I CTCTTC 4 cut(s) 27, 474, 620, 632
BstAUI TGTACA 1 cut(s) 450
BstDEI CTNAG 2 cut(s) 98, 130
BstF5I GGATG 3 cut(s) 370, 412, 543
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 1 cut(s) 353
BstMAI GTCTC 5 cut(s) 17, 88, 186, 208, 634
BstMBI GATC 1 cut(s) 350
BstMWI GCNNNNNNNGC 1 cut(s) 439
BstNI CCWGG 2 cut(s) 256, 671
BstNSI RCATGY 1 cut(s) 163
BstSCI CCNGG 4 cut(s) 254, 426, 571, 669
BsuRI GGCC 3 cut(s) 442, 494, 536
BtgZI GCGATG 1 cut(s) 176
BtsCI GGATG 3 cut(s) 370, 412, 543
BtsI GCAGTG 1 cut(s) 83
BtsIMutI CAGTG 4 cut(s) 83, 360, 421, 585
BveI ACCTGC 1 cut(s) 281
CfoI GCGC 1 cut(s) 290
Cfr13I GGNCC 2 cut(s) 424, 534
Csp6I GTAC 3 cut(s) 334, 451, 630
CviAII CATG 3 cut(s) 160, 496, 579
CviJI RGCY 9 cut(s) 53, 134, 433, 442, 494, 536, 555, 583, 669
CviKI_1 RGCY 9 cut(s) 53, 134, 433, 442, 494, 536, 555, 583, 669
CviQI GTAC 3 cut(s) 334, 451, 630
DdeI CTNAG 2 cut(s) 98, 130
DpnI GATC 1 cut(s) 352
DpnII GATC 1 cut(s) 350
EaeI YGGCCR 2 cut(s) 440, 492
Eam1104I CTCTTC 4 cut(s) 27, 474, 620, 632
EarI CTCTTC 4 cut(s) 27, 474, 620, 632
Eco31I GGTCTC 2 cut(s) 208, 634
Eco47I GGWCC 1 cut(s) 424
Eco57I CTGAAG 1 cut(s) 39
EcoRI GAATTC 1 cut(s) 259
EcoRII CCWGG 2 cut(s) 254, 669
Esp3I CGTCTC 2 cut(s) 17, 186
FaeI CATG 3 cut(s) 163, 499, 582
FaiI YATR 8 cut(s) 20, 161, 401, 497, 580, 593, 609, 662
FaqI GGGAC 1 cut(s) 555
FatI CATG 3 cut(s) 159, 495, 578
FokI GGATG 3 cut(s) 377, 419, 530
GlaI GCGC 1 cut(s) 289
HaeIII GGCC 3 cut(s) 442, 494, 536
HapII CCGG 4 cut(s) 213, 317, 427, 573
HhaI GCGC 1 cut(s) 290
Hin1II CATG 3 cut(s) 163, 499, 582
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HindIII AAGCTT 1 cut(s) 553
HinfI GANTC 7 cut(s) 96, 111, 141, 171, 187, 568, 680
HpaII CCGG 4 cut(s) 213, 317, 427, 573
HphI GGTGA 4 cut(s) 244, 305, 306, 577
Hpy166II GTNNAC 1 cut(s) 509
Hpy188I TCNGA 5 cut(s) 58, 101, 186, 265, 685
Hpy188III TCNNGA 2 cut(s) 213, 231
Hpy8I GTNNAC 1 cut(s) 509
HpyAV CCTTC 4 cut(s) 316, 388, 493, 649
HpyCH4III ACNGT 2 cut(s) 416, 450
HpyCH4V TGCA 3 cut(s) 76, 165, 578
HpyF10VI GCNNNNNNNGC 1 cut(s) 439
HpyF3I CTNAG 2 cut(s) 98, 130
Hsp92II CATG 3 cut(s) 163, 499, 582
HspAI GCGC 1 cut(s) 288
Kpn2I TCCGGA 1 cut(s) 212
Kzo9I GATC 1 cut(s) 350
LmnI GCTCC 1 cut(s) 666
MalI GATC 1 cut(s) 352
MboI GATC 1 cut(s) 350
MboII GAAGA 7 cut(s) 41, 44, 303, 491, 634, 637, 649
MlsI TGGCCA 1 cut(s) 494
MluCI AATT 2 cut(s) 146, 259
MluNI TGGCCA 1 cut(s) 494
MlyI GAGTC 4 cut(s) 90, 120, 577, 689
MmeI TCCRAC 1 cut(s) 663
MnlI CCTC 6 cut(s) 30, 260, 319, 415, 475, 641
Mox20I TGGCCA 1 cut(s) 494
MroI TCCGGA 1 cut(s) 212
MscI TGGCCA 1 cut(s) 494
MslI CAYNNNNRTG 1 cut(s) 590
Msp20I TGGCCA 1 cut(s) 494
MspI CCGG 4 cut(s) 213, 317, 427, 573
MspR9I CCNGG 4 cut(s) 256, 428, 573, 671
MvaI CCWGG 2 cut(s) 256, 671
MwoI GCNNNNNNNGC 1 cut(s) 439
NciI CCSGG 2 cut(s) 428, 573
NdeII GATC 1 cut(s) 350
NlaIII CATG 3 cut(s) 163, 499, 582
NlaIV GGNNCC 1 cut(s) 668
NspI RCATGY 1 cut(s) 163
PaqCI CACCTGC 1 cut(s) 281
PfeI GAWTC 3 cut(s) 141, 171, 187
PleI GAGTC 4 cut(s) 90, 119, 576, 688
PpsI GAGTC 4 cut(s) 90, 119, 576, 688
Psp6I CCWGG 2 cut(s) 254, 669
PspGI CCWGG 2 cut(s) 254, 669
PspN4I GGNNCC 1 cut(s) 668
PspPI GGNCC 2 cut(s) 424, 534
RsaI GTAC 3 cut(s) 335, 452, 631
RsaNI GTAC 3 cut(s) 334, 451, 630
RseI CAYNNNNRTG 1 cut(s) 590
Sau3AI GATC 1 cut(s) 350
Sau96I GGNCC 2 cut(s) 424, 534
SchI GAGTC 4 cut(s) 90, 120, 577, 689
ScrFI CCNGG 4 cut(s) 256, 428, 573, 671
SetI ASST 5 cut(s) 55, 295, 335, 399, 557
SinI GGWCC 1 cut(s) 424
SmiMI CAYNNNNRTG 1 cut(s) 590
Sse9I AATT 2 cut(s) 146, 259
SsiI CCGC 1 cut(s) 200
StyD4I CCNGG 4 cut(s) 254, 426, 571, 669
TaaI ACNGT 2 cut(s) 416, 450
TaqI TCGA 1 cut(s) 464
TasI AATT 2 cut(s) 146, 259
TatI WGTACW 1 cut(s) 450
TfiI GAWTC 3 cut(s) 141, 171, 187
TscAI CASTG 4 cut(s) 83, 360, 421, 592
TspDTI ATGAA 3 cut(s) 179, 512, 624
TspGWI ACGGA 1 cut(s) 41
TspRI CASTG 4 cut(s) 83, 360, 421, 592
VpaK11BI GGWCC 1 cut(s) 424
XapI RAATTY 2 cut(s) 146, 259
XceI RCATGY 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.