Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005251

Physical Location & Seq

Genomic Coordinates Forward (+)

1051697 .. 1056506

Gene Structure 4810 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1010300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1482 bp

ATGCAAAGTGTTGGTGGGGGCAAGTGCTTTTGGGGGTTTAGAAGTTTGATTCAGAGGAAGGAAGTTGATTCAGTTCATGGGGGGAGGAGGGAGGGGCATCATCAATTGGCTAGGATATTATCTGTCACTGACCTCATTGCCATTGGAGTTGGAGCTACAATAGGTGCTGGAGTTTATGTTCTTGTCGGCACAGTTGCTAGAGAGTGTGCAGGACCTGCACTTACCATTTCATTCTTAATTGCTGGGATTGCTGCTGCACTCTCAGCATTTTGTTATGCAGAGCTTGCGTGTCGTTGCCCATCTGCTGGGAGTGCCTATCACTATTCATACATATGCGTTGGAGAAGGTGTTGCTTGGTTGGTAGGTTGGGCTCTGATTCTAGAATATACCATTGGTGGTGCTGCTGTTGCTCGTGGCATAACCCCAAATCTGGCCTTGTTTTTTGGAGGATTGGATAAGTTACCTTCATTCTTGGCCCGTCAAACCATCCCCAGGCTTGGTATTGTGGTTGATCCATGTGCAGCAGTGTTAGTTCTTATTGTCGCTTTTCTCTTGTGCACAGGGATAAAGGAGAGTTCATTGGTACAAATGATTGTTACGTCAGTAAATGTTTCTGTCATGCTATTCATCATGATAGCAGGTGGCTATTTGGGTTTCAAAAATGGATGGGTTGGATATGAACTTTCTAGCGGGTACTTTCCCTTTGGAATAAATGGGATGTTTGCTGGTTCTGCTGTGGTCTTCTTTTCTTACATTGGTTTTGATTCAGTTACTAGCACAGTTGAGGAGGTGAGGAATCCTCAACGAGATTTGCCACTCGGTATTGGAATAGCATTGTTTATATGTTGCATCTTGTACATGTTTGTATCTTTTGTAATTACCGGCTTAGTACCATACTATGACTTAGATCCAGATACTCCCATTTCCTCAGTATTTGCAAGCTATGGGGTGGAATGGGCAGTGTATATTATAACAACGGGAGCAGTTACTGCTCTTTCTGCAAGTTTGTTGGGTTCGCTTCTTGCTCAGCCACGGATCCTGATGGCAATGGCTAGAGATGGATTGTTACCATCATTCTTTTCAGACATTAATAAAAAAACCCAAGTTCCTGTGAAGAGCACTGTTACAACTGGTATTTTTGCCGCAGTTCTGGCATTCTTTATGGATGTTTCCCAATTGGCAGGGATGGTTAGCGTTGGTACACTATTTGCGTTTACAACTGCTGCACTTTCAGTTTTGATACTACGATATGTGCCACCAGATGAAGTGCCACTTTCATCAGCACTTAAAGAGTCCATTACGTCAACATCATTGCAATCTGGTCGTAATATTCAGGAAAGTGACAGCGAAAACCTTCCACTTCCTGCTGGCTCCTCTAAGGATGATAGTCAATATTTACGTGAAATAGGGGAGGCATCACTTGGCTATCCTCTGATTCAGAAAGTCTTATCACAAGGTAATAGTAGCTCACGTGATTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0009705 GO:0016020 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

AA_permease_2 AA_permease_C

Protein Analysis

494

Amino Acids

52.23

Weight (kDa)

5.49

Isoelectric Point (pI)

31.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_permease_2	PF13520	40 - 422	2e-53	Amino acid permease
AA_permease	PF00324	45 - 413	5.9e-38	Amino acid permease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005251)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03720
malus_domestica	MD12G1010300.v1.1 MD12G1010400.v1.1 MD14G1007500.v1.1
prunus_persica	Prupe.7G012400_v2.0.a1 Prupe.7G012400_v2.0.a1 Prupe.7G012400_v2.0.a1 Prupe.7G012400_v2.0.a1 Prupe.7G012400_v2.0.a1 Prupe.7G012400_v2.0.a1 Prupe.7G012400_v2.0.a1 Prupe.7G012400_v2.0.a1 Prupe.7G012400_v2.0.a1 Prupe.7G012400_v2.0.a1
pyrus_communis	pycom12g00800
rosa_chinensis	RchiOBHm_Chr3g0496181
rosa_laevigata	RLG00000022554
rosa_multiflora	Rmu_sc0003733.1_g000063 Rmu_sc0006695.1_g000009
rosa_roxburghii	Rroxscaffold_6G00389490
rosa_rugosa	Rorug03G0280100.1 Rorug03G0280200 Rorug03G0280300 Rorug03G0280300
rosa_samantha	Rh3AG327100 Rh3BG363300 Rh3CG360000 Rh3DG363200
rosa_wichuraiana	Rw3G028670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	971
AarI	CACCTGC	1 cut(s)	629
Acc36I	ACCTGC	2 cut(s)	223, 629
AccB7I	CCANNNNNTGG	1 cut(s)	305
AciI	CCGC	2 cut(s)	690, 1143
AclWI	GGATC	4 cut(s)	506, 902, 1030, 1043
AcvI	CACGTG	1 cut(s)	1472
AfaI	GTAC	5 cut(s)	585, 695, 857, 891, 1201
AfiI	CCNNNNNNNGG	4 cut(s)	305, 430, 492, 497
AflIII	ACRYGT	1 cut(s)	858
AgsI	TTSAA	1 cut(s)	658
AjnI	CCWGG	1 cut(s)	491
AjuI	GAANNNNNNNTTGG	2 cut(s)	375, 407
AloI	GAACNNNNNNTCC	2 cut(s)	162, 194
AluBI	AGCT	4 cut(s)	155, 283, 942, 1467
AluI	AGCT	4 cut(s)	155, 283, 942, 1467
Alw21I	GWGCWC	2 cut(s)	560, 1121
Alw44I	GTGCAC	1 cut(s)	556
AlwI	GGATC	4 cut(s)	506, 902, 1030, 1043
AlwNI	CAGNNNCTG	2 cut(s)	215, 989
AoxI	GGCC	2 cut(s)	432, 474
ApaLI	GTGCAC	1 cut(s)	556
ApeKI	GCWGC	5 cut(s)	251, 254, 401, 521, 1223
AseI	ATTAAT	1 cut(s)	1089
Asp700I	GAANNNNTTC	1 cut(s)	1353
AspS9I	GGNCC	2 cut(s)	212, 475
AsuHPI	GGTGA	1 cut(s)	802
AvaII	GGWCC	1 cut(s)	212
BaeGI	GKGCMC	1 cut(s)	560
BamHI	GGATCC	1 cut(s)	1035
BanII	GRGCYC	1 cut(s)	373
BauI	CACGAG	1 cut(s)	411
BbrPI	CACGTG	1 cut(s)	1472
BbsI	GAAGAC	1 cut(s)	733
Bbv12I	GWGCWC	2 cut(s)	560, 1121
BbvI	GCAGC	5 cut(s)	238, 241, 388, 533, 1210
BccI	CCATC	7 cut(s)	307, 494, 660, 1036, 1052, 1078, 1180
BcgI	CGANNNNNNTGC	2 cut(s)	1304, 1338
BciT130I	CCWGG	1 cut(s)	493
BfaI	CTAG	6 cut(s)	111, 198, 380, 687, 774, 1053
BfuAI	ACCTGC	2 cut(s)	223, 629
BisI	GCNGC	6 cut(s)	252, 255, 402, 522, 1143, 1224
BlpI	GCTNAGC	1 cut(s)	1026
BlsI	GCNGC	6 cut(s)	253, 256, 403, 523, 1144, 1225
Bme1390I	CCNGG	1 cut(s)	493
Bme18I	GGWCC	1 cut(s)	212
BmgT120I	GGNCC	2 cut(s)	212, 475
BmiI	GGNNCC	2 cut(s)	1037, 1372
BmrFI	CCNGG	1 cut(s)	493
BmsI	GCATC	3 cut(s)	106, 858, 1424
BpiI	GAAGAC	1 cut(s)	733
BplI	GAGNNNNNCTC	2 cut(s)	784, 816
BpmI	CTGGAG	1 cut(s)	189
Bpu1102I	GCTNAGC	1 cut(s)	1026
BsaAI	YACGTR	2 cut(s)	1400, 1472
BsaJI	CCNNGG	2 cut(s)	491, 1031
BsaXI	ACNNNNNCTCC	4 cut(s)	162, 192, 333, 363
Bsc4I	CCNNNNNNNGG	4 cut(s)	305, 430, 492, 497
Bse118I	RCCGGY	1 cut(s)	881
Bse1I	ACTGG	1 cut(s)	1135
Bse3DI	GCAATG	3 cut(s)	135, 1053, 1310
BseBI	CCWGG	1 cut(s)	493
BseDI	CCNNGG	2 cut(s)	491, 1031
BseGI	GGATG	6 cut(s)	486, 671, 723, 1171, 1191, 1387
BseLI	CCNNNNNNNGG	4 cut(s)	305, 430, 492, 497
BseMI	GCAATG	3 cut(s)	135, 1053, 1310
BseMII	CTCAG	3 cut(s)	276, 942, 1040
BseNI	ACTGG	1 cut(s)	1135
BseRI	GAGGAG	3 cut(s)	100, 800, 1363
BseSI	GKGCMC	1 cut(s)	560
BseXI	GCAGC	5 cut(s)	238, 241, 388, 533, 1210
BseYI	CCCAGC	2 cut(s)	242, 305
BsgI	GTGCAG	5 cut(s)	201, 228, 240, 540, 1209
BshFI	GGCC	2 cut(s)	434, 476
BsiHKAI	GWGCWC	2 cut(s)	560, 1121
BsiSI	CCGG	1 cut(s)	882
BslI	CCNNNNNNNGG	4 cut(s)	305, 430, 492, 497
BsmI	GAATGC	1 cut(s)	1154
BsnI	GGCC	2 cut(s)	434, 476
Bsp1286I	GDGCHC	3 cut(s)	373, 560, 1121
Bsp1407I	TGTACA	1 cut(s)	855
Bsp143I	GATC	3 cut(s)	511, 907, 1035
Bsp1720I	GCTNAGC	1 cut(s)	1026
BspACI	CCGC	2 cut(s)	690, 1143
BspANI	GGCC	2 cut(s)	434, 476
BspCNI	CTCAG	3 cut(s)	275, 941, 1039
BspHI	TCATGA	1 cut(s)	630
BspLI	GGNNCC	2 cut(s)	1037, 1372
BspMI	ACCTGC	2 cut(s)	223, 629
BspPI	GGATC	4 cut(s)	506, 902, 1030, 1043
BspQI	GCTCTTC	1 cut(s)	1109
BsrDI	GCAATG	3 cut(s)	135, 1053, 1310
BsrFI	RCCGGY	1 cut(s)	881
BsrGI	TGTACA	1 cut(s)	855
BsrI	ACTGG	1 cut(s)	1135
BssAI	RCCGGY	1 cut(s)	881
BssECI	CCNNGG	2 cut(s)	491, 1031
BssMI	GATC	3 cut(s)	511, 907, 1035
BssSI	CACGAG	1 cut(s)	411
Bst2BI	CACGAG	1 cut(s)	411
Bst2UI	CCWGG	1 cut(s)	493
Bst4CI	ACNGT	3 cut(s)	193, 781, 1123
Bst6I	CTCTTC	1 cut(s)	1109
BstAPI	GCANNNNNTGC	3 cut(s)	215, 284, 989
BstAUI	TGTACA	1 cut(s)	855
BstBAI	YACGTR	2 cut(s)	1400, 1472
BstC8I	GCNNGC	3 cut(s)	285, 940, 1369
BstDEI	CTNAG	6 cut(s)	262, 886, 904, 928, 1026, 1377
BstDSI	CCRYGG	1 cut(s)	1031
BstF5I	GGATG	6 cut(s)	486, 671, 723, 1171, 1191, 1387
BstKTI	GATC	3 cut(s)	514, 910, 1038
BstMBI	GATC	3 cut(s)	511, 907, 1035
BstNI	CCWGG	1 cut(s)	493
BstNSI	RCATGY	1 cut(s)	862
BstSCI	CCNGG	1 cut(s)	491
BstSLI	GKGCMC	1 cut(s)	560
BstV1I	GCAGC	5 cut(s)	238, 241, 388, 533, 1210
BstV2I	GAAGAC	1 cut(s)	733
BstX2I	RGATCY	2 cut(s)	907, 1035
BstYI	RGATCY	2 cut(s)	907, 1035
BsuRI	GGCC	2 cut(s)	434, 476
BtgI	CCRYGG	1 cut(s)	1031
BtsCI	GGATG	6 cut(s)	486, 671, 723, 1171, 1191, 1387
BtsI	GCAGTG	2 cut(s)	531, 966
BtsIMutI	CAGTG	4 cut(s)	126, 531, 966, 1119
BveI	ACCTGC	2 cut(s)	223, 629
Cac8I	GCNNGC	3 cut(s)	285, 940, 1369
CaiI	CAGNNNCTG	2 cut(s)	215, 989
CciI	TCATGA	1 cut(s)	630
Cfr10I	RCCGGY	1 cut(s)	881
Cfr13I	GGNCC	2 cut(s)	212, 475
Csp6I	GTAC	5 cut(s)	584, 694, 856, 890, 1200
CviAII	CATG	5 cut(s)	77, 516, 619, 631, 859
CviQI	GTAC	5 cut(s)	584, 694, 856, 890, 1200
DdeI	CTNAG	6 cut(s)	262, 886, 904, 928, 1026, 1377
DpnI	GATC	3 cut(s)	513, 909, 1037
DpnII	GATC	3 cut(s)	511, 907, 1035
Eam1104I	CTCTTC	1 cut(s)	1109
EarI	CTCTTC	1 cut(s)	1109
Eco24I	GRGCYC	1 cut(s)	373
Eco47I	GGWCC	1 cut(s)	212
Eco72I	CACGTG	1 cut(s)	1472
EcoO109I	RGGNCCY	1 cut(s)	212
EcoRII	CCWGG	1 cut(s)	491
EcoT38I	GRGCYC	1 cut(s)	373
FaeI	CATG	5 cut(s)	80, 519, 622, 634, 862
FalI	AAGNNNNNCTT	2 cut(s)	1257, 1289
FatI	CATG	5 cut(s)	76, 515, 618, 630, 858
FauI	CCCGC	1 cut(s)	683
FauNDI	CATATG	1 cut(s)	332
Fnu4HI	GCNGC	6 cut(s)	252, 255, 402, 522, 1143, 1224
FokI	GGATG	6 cut(s)	473, 678, 730, 1178, 1198, 1394
FriOI	GRGCYC	1 cut(s)	373
Fsp4HI	GCNGC	6 cut(s)	252, 255, 402, 522, 1143, 1224
FspBI	CTAG	6 cut(s)	111, 198, 380, 687, 774, 1053
GluI	GCNGC	6 cut(s)	252, 255, 402, 522, 1143, 1224
GsaI	CCCAGC	2 cut(s)	246, 309
GsuI	CTGGAG	1 cut(s)	189
HaeIII	GGCC	2 cut(s)	434, 476
HapII	CCGG	1 cut(s)	882
Hin1II	CATG	5 cut(s)	80, 519, 622, 634, 862
HincII	GTYRAC	1 cut(s)	1305
HindII	GTYRAC	1 cut(s)	1305
HinfI	GANTC	7 cut(s)	49, 68, 376, 764, 796, 1292, 1435
HpaII	CCGG	1 cut(s)	882
HphI	GGTGA	1 cut(s)	802
Hpy166II	GTNNAC	4 cut(s)	558, 1202, 1215, 1305
Hpy188I	TCNGA	5 cut(s)	54, 375, 1084, 1434, 1440
Hpy188III	TCNNGA	5 cut(s)	380, 631, 911, 1039, 1334
Hpy8I	GTNNAC	4 cut(s)	558, 1202, 1215, 1305
HpyAV	CCTTC	4 cut(s)	52, 338, 474, 1364
HpyCH4III	ACNGT	3 cut(s)	193, 781, 1123
HpyCH4IV	ACGT	4 cut(s)	599, 1301, 1399, 1471
HpyF3I	CTNAG	6 cut(s)	262, 886, 904, 928, 1026, 1377
HpySE526I	ACGT	4 cut(s)	599, 1301, 1399, 1471
Hsp92II	CATG	5 cut(s)	80, 519, 622, 634, 862
Kzo9I	GATC	3 cut(s)	511, 907, 1035
LguI	GCTCTTC	1 cut(s)	1109
LmnI	GCTCC	3 cut(s)	152, 980, 1376
Lsp1109I	GCAGC	5 cut(s)	238, 241, 388, 533, 1210
LweI	GCATC	3 cut(s)	106, 858, 1424
MaeI	CTAG	6 cut(s)	111, 198, 380, 687, 774, 1053
MaeII	ACGT	4 cut(s)	599, 1301, 1399, 1471
MaeIII	GTNAC	8 cut(s)	124, 459, 595, 769, 985, 1065, 1123, 1340
MalI	GATC	3 cut(s)	513, 909, 1037
MboI	GATC	3 cut(s)	511, 907, 1035
MboII	GAAGA	2 cut(s)	733, 1126
MfeI	CAATTG	2 cut(s)	104, 1175
MflI	RGATCY	2 cut(s)	907, 1035
MhlI	GDGCHC	3 cut(s)	373, 560, 1121
MluCI	AATT	4 cut(s)	104, 237, 876, 1175
MlyI	GAGTC	1 cut(s)	1301
MmeI	TCCRAC	3 cut(s)	130, 319, 652
MroXI	GAANNNNTTC	1 cut(s)	1353
MseI	TTAA	3 cut(s)	236, 1089, 1287
MslI	CAYNNNNRTG	1 cut(s)	331
MspI	CCGG	1 cut(s)	882
MspR9I	CCNGG	1 cut(s)	493
MunI	CAATTG	2 cut(s)	104, 1175
Mva1269I	GAATGC	1 cut(s)	1154
MvaI	CCWGG	1 cut(s)	493
NdeI	CATATG	1 cut(s)	332
NdeII	GATC	3 cut(s)	511, 907, 1035
NlaIII	CATG	5 cut(s)	80, 519, 622, 634, 862
NlaIV	GGNNCC	2 cut(s)	1037, 1372
NmuCI	GTSAC	2 cut(s)	124, 1340
NspI	RCATGY	1 cut(s)	862
PagI	TCATGA	1 cut(s)	630
PaqCI	CACCTGC	1 cut(s)	629
PciI	ACATGT	1 cut(s)	858
PciSI	GCTCTTC	1 cut(s)	1109
PctI	GAATGC	1 cut(s)	1154
PdmI	GAANNNNTTC	1 cut(s)	1353
PfeI	GAWTC	6 cut(s)	49, 68, 376, 764, 796, 1435
PflMI	CCANNNNNTGG	1 cut(s)	305
PkrI	GCNGC	6 cut(s)	253, 256, 403, 523, 1144, 1225
PleI	GAGTC	1 cut(s)	1300
PmaCI	CACGTG	1 cut(s)	1472
PmlI	CACGTG	1 cut(s)	1472
PpsI	GAGTC	1 cut(s)	1300
Ppu21I	YACGTR	2 cut(s)	1400, 1472
PpuMI	RGGWCCY	1 cut(s)	212
PscI	ACATGT	1 cut(s)	858
PshBI	ATTAAT	1 cut(s)	1089
PsiI	TTATAA	1 cut(s)	971
Psp5II	RGGWCCY	1 cut(s)	212
Psp6I	CCWGG	1 cut(s)	491
PspCI	CACGTG	1 cut(s)	1472
PspFI	CCCAGC	2 cut(s)	242, 305
PspGI	CCWGG	1 cut(s)	491
PspN4I	GGNNCC	2 cut(s)	1037, 1372
PspPI	GGNCC	2 cut(s)	212, 475
PspPPI	RGGWCCY	1 cut(s)	212
PstNI	CAGNNNCTG	2 cut(s)	215, 989
PsuI	RGATCY	2 cut(s)	907, 1035
RsaI	GTAC	5 cut(s)	585, 695, 857, 891, 1201
RsaNI	GTAC	5 cut(s)	584, 694, 856, 890, 1200
RseI	CAYNNNNRTG	1 cut(s)	331
SapI	GCTCTTC	1 cut(s)	1109
SaqAI	TTAA	3 cut(s)	236, 1089, 1287
SatI	GCNGC	6 cut(s)	252, 255, 402, 522, 1143, 1224
Sau3AI	GATC	3 cut(s)	511, 907, 1035
Sau96I	GGNCC	2 cut(s)	212, 475
SchI	GAGTC	1 cut(s)	1301
ScrFI	CCNGG	1 cut(s)	493
SduI	GDGCHC	3 cut(s)	373, 560, 1121
SfaNI	GCATC	3 cut(s)	106, 858, 1424
SinI	GGWCC	1 cut(s)	212
SmiMI	CAYNNNNRTG	1 cut(s)	331
Sse9I	AATT	4 cut(s)	104, 237, 876, 1175
SsiI	CCGC	2 cut(s)	690, 1143
SspI	AATATT	2 cut(s)	1330, 1394
SspMI	CTAG	6 cut(s)	111, 198, 380, 687, 774, 1053
StyD4I	CCNGG	1 cut(s)	491
TaaI	ACNGT	3 cut(s)	193, 781, 1123
TaiI	ACGT	4 cut(s)	602, 1304, 1402, 1474
TasI	AATT	4 cut(s)	104, 237, 876, 1175
TatI	WGTACW	1 cut(s)	855
TauI	GCSGC	1 cut(s)	1145
TfiI	GAWTC	6 cut(s)	49, 68, 376, 764, 796, 1435
Tru1I	TTAA	3 cut(s)	236, 1089, 1287
Tru9I	TTAA	3 cut(s)	236, 1089, 1287
TscAI	CASTG	4 cut(s)	133, 531, 966, 1126
TseFI	GTSAC	2 cut(s)	124, 1340
TseI	GCWGC	5 cut(s)	251, 254, 401, 521, 1223
Tsp45I	GTSAC	2 cut(s)	124, 1340
TspDTI	ATGAA	9 cut(s)	65, 219, 315, 456, 567, 616, 693, 1266, 1278
TspGWI	ACGGA	1 cut(s)	1048
TspRI	CASTG	4 cut(s)	133, 531, 966, 1126
Van91I	CCANNNNNTGG	1 cut(s)	305
VneI	GTGCAC	1 cut(s)	556
VpaK11BI	GGWCC	1 cut(s)	212
VspI	ATTAAT	1 cut(s)	1089
XbaI	TCTAGA	1 cut(s)	379
XceI	RCATGY	1 cut(s)	862
XmnI	GAANNNNTTC	1 cut(s)	1353
XspI	CTAG	6 cut(s)	111, 198, 380, 687, 774, 1053

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.