MD12G1011500.v1.1

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013911
Physical Location & Seq
Forward (+)
1189557 .. 1191302
1746 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1011500.v1.1.491

Sequence Viewer

Length: 1746 bp
ATGGCAAGCTCAACCTCCATTCCTCAACTCAAGAAACTCTCCCTATTCATCAACATTTCCTTTCTCTTTCTCTTATGTCTCTCTCTCATCACAACATATCTCCATCCTAATAACTTAGACTTACCAAACCACCCTAAATCCAATAGTATTAGTAGTGCAAATATTCTCAGTGAGGATATCAAATCAGTTAATGGCTGCAAAGATCTCCATCAACACGCAGATTATAAATCCAGGTGTTTGTATGTGAAAACCCACACAGGTTGCAACCCTAAAGGGTATATACATTATCTGAAAATATTTTACTGCAGTCTTGGCAAGTTTCCAATTCTGGGTCATGCTGCAGTTCTACTTTGGCTTATTGTTTTATTCTATCTATTGGGCAACACAGCTGCTAATTATTTCTGTTCCTCCCTAGAGAGTTTGTCCAATATCTTGAAGCTCTCTCCTACCATCGCCGGCGTCACCCTCCTCTCCCTTGGGAACGGGGCCTCCGATGTTTTTTCCAGCATCGTTGCGTTCACAAAATCCGCAGACGGCGATGTTGGCCTCAACAGTGTATTAGGGGGTGCCTTCTTCGTGTCCAGCATTGTGGTGGGAGTTATTAGTATACTAATAAGCCCTCACCAGGTTTCAGTTGATGAGGCCAGCTTTGTCAGGGATGTCATCTTCTTCTTATTCTCTCTCTCATCCCTTCTGGCAATCATAGTCATTGGGAGGATCAACTTGTGGGGTGCCCTTTCTTTTCTTGCTATTTACTTTGTTTATGTTGGTACTGTTTCAGTATCTCAAATATACAAAAGGAACAAAAATCTTGCAGCTTCAAAGTATTTACCAGTGGTGCAAAGTTTTGATGATTTAGCGGATGGTACTGCAAGTATACCATTACTTGGTTATGTTGATGATGATAAACCCGTTTCCAACGCAAATTTGAAAACGGGACTTCATGATGATCAAGAACAAAATTATTGGTTTTTTTATTTGGGAAAATTGATTTATATCTTGGAGTTACCTCTCTACTTGCCTAGAAGATTGACTATACCTGTTGTGAGCGAAGAAAGATGGTCTAAGCCATATGCAGTTATTTCTGTAGCTTTGGCACCAATTTTACTGGCAGCCCTTTGTAACACACAGAGGGAAGATCTGAGTTCAAGAGGTAAGTTGGTAACTTACATGACAGCAGTGCTGATTGGGTTAATTTTGAGTAACCTTGCATACGTGACTACAAATAAGTGTAGCCCACCAAAGGAGTGCTTGTTCCCTTGGCTTGTTGGTGGGTTTTTGATGAGTGTAGCTTGGACGTATATTACTGCTGAAGAGTTGGTTGGCTTGTTAGAATCATATGGTAGTATTTTAGGGATAAGCCCTTCAATTCTAGGACTTACTGTCCTAGCTTGGGGTAACTCACTCAATGATTTGATAGCTAATGCTACAATGGCAGTGAAGGGTGGGACAGATGGGGCCCAAATTGCTATTTCAGGATGCTATGCGGGGCCCATGTTTAACACATTGGTGGGAGTGGGAACGTCACTGGTTCTGTCATCATGGTTTGAGTATCCATCCCCATATGTCATCCCTACCGACCATTCCCTCTACGAAACAATAGGGTTTTTGATGGGTGGTTTACTTTGGGCACTTGTGATCTTGCCAAAGAAGGGTATGAAGCTGGATAGATATATGGGGAGTGGCCTTGTGGCAATTTACTTGTGTTTTCTGTTTGTGAGGTTAGCCAGGCTCTTGTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_Ca_ex

Protein Analysis

582

Amino Acids

63.37

Weight (kDa)

7.9

Isoelectric Point (pI)

34.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 116 - 259 6.7e-24 Sodium/calcium exchanger protein
Na_Ca_ex PF01699 422 - 575 2.7e-29 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013911)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17860 AT5G17860
fragaria_vesca FvH4_4g12810
malus_domestica MD12G1011500.v1.1
prunus_persica Prupe.7G014900_v2.0.a1 Prupe.7G014900_v2.0.a1
pyrus_communis pycom12g00900
rosa_chinensis RchiOBHm_Chr3g0496071
rosa_laevigata RLG00000022565
rosa_multiflora Rmu_sc0005781.1_g000006
rosa_roxburghii Rroxscaffold_6G00389590
rosa_rugosa Rorug03G0279300
rosa_samantha Rh3AG326400 Rh3BG362700 Rh3CG358800 Rh3DG362100
rosa_wichuraiana Rw3G028580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 225
AccB1I GGYRCC 3 cut(s) 566, 731, 1096
AccB7I CCANNNNNTGG 1 cut(s) 887
AccI GTMKAC 2 cut(s) 607, 877
AciI CCGC 3 cut(s) 528, 860, 1487
AclWI GGATC 1 cut(s) 725
AcsI RAATTY 1 cut(s) 925
AcuI CTGAAG 1 cut(s) 1332
AcyI GRCGYC 1 cut(s) 459
AfaI GTAC 2 cut(s) 772, 868
AfiI CCNNNNNNNGG 6 cut(s) 329, 625, 887, 1243, 1393, 1652
AgsI TTSAA 5 cut(s) 436, 822, 931, 1149, 1368
AhdI GACNNNNNGTC 1 cut(s) 1382
AjnI CCWGG 3 cut(s) 230, 624, 1727
AjuI GAANNNNNNNTTGG 2 cut(s) 1305, 1337
AleI CACNNNNGTG 1 cut(s) 1508
AloI GAACNNNNNNTCC 4 cut(s) 473, 505, 1238, 1270
Alw26I GTCTC 1 cut(s) 83
AlwI GGATC 1 cut(s) 725
AoxI GGCC 6 cut(s) 486, 544, 642, 1458, 1490, 1684
ApaI GGGCCC 2 cut(s) 1462, 1494
ApeKI GCWGC 5 cut(s) 195, 338, 389, 815, 1112
ApoI RAATTY 1 cut(s) 925
AspS9I GGNCC 5 cut(s) 486, 1458, 1459, 1490, 1491
AsuHPI GGTGA 2 cut(s) 454, 614
BaeGI GKGCMC 4 cut(s) 736, 1462, 1494, 1633
BanI GGYRCC 3 cut(s) 566, 731, 1096
BanII GRGCYC 2 cut(s) 1462, 1494
BbvI GCAGC 5 cut(s) 182, 325, 376, 827, 1124
BccI CCATC 8 cut(s) 111, 216, 458, 857, 1053, 1448, 1564, 1606
BceAI ACGGC 1 cut(s) 550
BciT130I CCWGG 3 cut(s) 232, 626, 1729
BciVI GTATCC 1 cut(s) 1563
BclI TGATCA 1 cut(s) 949
BcoDI GTCTC 1 cut(s) 83
BfaI CTAG 4 cut(s) 413, 1023, 1373, 1388
BfmI CTRYAG 3 cut(s) 304, 339, 1086
BfuI GTATCC 1 cut(s) 1563
BglII AGATCT 2 cut(s) 202, 1138
BisI GCNGC 5 cut(s) 196, 339, 390, 816, 1113
BlsI GCNGC 5 cut(s) 197, 340, 391, 817, 1114
Bme1390I CCNGG 3 cut(s) 232, 626, 1729
BmeRI GACNNNNNGTC 1 cut(s) 1382
BmgT120I GGNCC 5 cut(s) 486, 1458, 1459, 1490, 1491
BmiI GGNNCC 8 cut(s) 487, 568, 733, 1098, 1459, 1460, 1491, 1492
BmrFI CCNGG 3 cut(s) 232, 626, 1729
BmsI GCATC 2 cut(s) 516, 1469
BpuEI CTTGAG 1 cut(s) 14
BsaAI YACGTR 1 cut(s) 1216
BsaBI GATNNNNATC 2 cut(s) 207, 995
BsaHI GRCGYC 1 cut(s) 459
BsaJI CCNNGG 2 cut(s) 475, 1259
BsaXI ACNNNNNCTCC 4 cut(s) 473, 503, 1238, 1268
Bsc4I CCNNNNNNNGG 6 cut(s) 329, 625, 887, 1243, 1393, 1652
Bse118I RCCGGY 1 cut(s) 455
Bse1I ACTGG 3 cut(s) 833, 1113, 1533
Bse8I GATNNNNATC 2 cut(s) 207, 995
BseBI CCWGG 3 cut(s) 232, 626, 1729
BseDI CCNNGG 2 cut(s) 475, 1259
BseGI GGATG 7 cut(s) 103, 664, 686, 868, 1484, 1556, 1569
BseJI GATNNNNATC 2 cut(s) 207, 995
BseLI CCNNNNNNNGG 6 cut(s) 329, 625, 887, 1243, 1393, 1652
BseMII CTCAG 2 cut(s) 181, 1133
BseNI ACTGG 3 cut(s) 833, 1113, 1533
BseRI GAGGAG 1 cut(s) 458
BseSI GKGCMC 4 cut(s) 736, 1462, 1494, 1633
BseXI GCAGC 5 cut(s) 182, 325, 376, 827, 1124
BshFI GGCC 6 cut(s) 488, 546, 644, 1460, 1492, 1686
BshNI GGYRCC 3 cut(s) 566, 731, 1096
BsiSI CCGG 1 cut(s) 456
BslFI GGGAC 2 cut(s) 951, 1462
BslI CCNNNNNNNGG 6 cut(s) 329, 625, 887, 1243, 1393, 1652
BsmAI GTCTC 1 cut(s) 83
BsmFI GGGAC 2 cut(s) 951, 1462
BsnI GGCC 6 cut(s) 488, 546, 644, 1460, 1492, 1686
Bsp120I GGGCCC 2 cut(s) 1458, 1490
Bsp1286I GDGCHC 4 cut(s) 736, 1462, 1494, 1633
Bsp143I GATC 5 cut(s) 202, 717, 949, 1138, 1638
BspACI CCGC 3 cut(s) 528, 860, 1487
BspANI GGCC 6 cut(s) 488, 546, 644, 1460, 1492, 1686
BspCNI CTCAG 2 cut(s) 180, 1134
BspHI TCATGA 1 cut(s) 943
BspLI GGNNCC 8 cut(s) 487, 568, 733, 1098, 1459, 1460, 1491, 1492
BspMAI CTGCAG 2 cut(s) 308, 343
BspPI GGATC 1 cut(s) 725
BspT107I GGYRCC 3 cut(s) 566, 731, 1096
BsrFI RCCGGY 1 cut(s) 455
BsrI ACTGG 3 cut(s) 833, 1113, 1533
BssAI RCCGGY 1 cut(s) 455
BssECI CCNNGG 2 cut(s) 475, 1259
BssMI GATC 5 cut(s) 202, 717, 949, 1138, 1638
BssNAI GTATAC 2 cut(s) 608, 878
BssNI GRCGYC 1 cut(s) 459
BssT1I CCWWGG 2 cut(s) 475, 1259
Bst1107I GTATAC 2 cut(s) 608, 878
Bst2UI CCWGG 3 cut(s) 232, 626, 1729
Bst4CI ACNGT 3 cut(s) 554, 775, 1384
Bst6I CTCTTC 1 cut(s) 1308
BstACI GRCGYC 1 cut(s) 459
BstBAI YACGTR 1 cut(s) 1216
BstC8I GCNNGC 3 cut(s) 7, 457, 646
BstDEI CTNAG 4 cut(s) 115, 167, 1065, 1142
BstF5I GGATG 7 cut(s) 103, 664, 686, 868, 1484, 1556, 1569
BstKTI GATC 5 cut(s) 205, 720, 952, 1141, 1641
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 5 cut(s) 202, 717, 949, 1138, 1638
BstMWI GCNNNNNNNGC 4 cut(s) 312, 543, 1433, 1466
BstNI CCWGG 3 cut(s) 232, 626, 1729
BstSCI CCNGG 3 cut(s) 230, 624, 1727
BstSFI CTRYAG 3 cut(s) 304, 339, 1086
BstSLI GKGCMC 4 cut(s) 736, 1462, 1494, 1633
BstV1I GCAGC 5 cut(s) 182, 325, 376, 827, 1124
BstX2I RGATCY 2 cut(s) 202, 1138
BstXI CCANNNNNNTGG 1 cut(s) 589
BstYI RGATCY 2 cut(s) 202, 1138
BstZ17I GTATAC 2 cut(s) 608, 878
BsuI GTATCC 1 cut(s) 1563
BsuRI GGCC 6 cut(s) 488, 546, 644, 1460, 1492, 1686
BtgZI GCGATG 2 cut(s) 436, 552
BtsCI GGATG 7 cut(s) 103, 664, 686, 868, 1484, 1556, 1569
BtsI GCAGTG 2 cut(s) 1185, 1443
BtsIMutI CAGTG 6 cut(s) 175, 559, 840, 1185, 1443, 1526
Cac8I GCNNGC 3 cut(s) 7, 457, 646
CciI TCATGA 1 cut(s) 943
Cfr10I RCCGGY 1 cut(s) 455
Cfr13I GGNCC 5 cut(s) 486, 1458, 1459, 1490, 1491
CseI GACGC 1 cut(s) 448
CsiI ACCWGGT 1 cut(s) 624
Csp6I GTAC 2 cut(s) 771, 867
CspCI CAANNNNNGTGG 2 cut(s) 242, 277
CviAII CATG 5 cut(s) 335, 944, 1171, 1495, 1542
CviQI GTAC 2 cut(s) 771, 867
DdeI CTNAG 4 cut(s) 115, 167, 1065, 1142
DpnI GATC 5 cut(s) 204, 719, 951, 1140, 1640
DpnII GATC 5 cut(s) 202, 717, 949, 1138, 1638
DriI GACNNNNNGTC 1 cut(s) 1382
Eam1104I CTCTTC 1 cut(s) 1308
Eam1105I GACNNNNNGTC 1 cut(s) 1382
EarI CTCTTC 1 cut(s) 1308
Eco130I CCWWGG 2 cut(s) 475, 1259
Eco24I GRGCYC 2 cut(s) 1462, 1494
Eco32I GATATC 1 cut(s) 178
Eco57I CTGAAG 1 cut(s) 1332
EcoO109I RGGNCCY 3 cut(s) 486, 1458, 1490
EcoRII CCWGG 3 cut(s) 230, 624, 1727
EcoRV GATATC 1 cut(s) 178
EcoT14I CCWWGG 2 cut(s) 475, 1259
EcoT38I GRGCYC 2 cut(s) 1462, 1494
ErhI CCWWGG 2 cut(s) 475, 1259
FaeI CATG 5 cut(s) 338, 947, 1174, 1498, 1545
FalI AAGNNNNNCTT 2 cut(s) 1235, 1267
FaqI GGGAC 2 cut(s) 951, 1462
FatI CATG 5 cut(s) 334, 943, 1170, 1494, 1541
FauI CCCGC 1 cut(s) 1480
FauNDI CATATG 3 cut(s) 1072, 1339, 1564
FbaI TGATCA 1 cut(s) 949
FblI GTMKAC 2 cut(s) 607, 877
Fnu4HI GCNGC 5 cut(s) 196, 339, 390, 816, 1113
FokI GGATG 7 cut(s) 90, 671, 673, 875, 1491, 1543, 1556
FriOI GRGCYC 2 cut(s) 1462, 1494
Fsp4HI GCNGC 5 cut(s) 196, 339, 390, 816, 1113
FspBI CTAG 4 cut(s) 413, 1023, 1373, 1388
GluI GCNGC 5 cut(s) 196, 339, 390, 816, 1113
HaeIII GGCC 6 cut(s) 488, 546, 644, 1460, 1492, 1686
HapII CCGG 1 cut(s) 456
HgaI GACGC 1 cut(s) 448
Hin1I GRCGYC 1 cut(s) 459
Hin1II CATG 5 cut(s) 338, 947, 1174, 1498, 1545
HinfI GANTC 1 cut(s) 1334
HpaII CCGG 1 cut(s) 456
HphI GGTGA 2 cut(s) 454, 614
Hpy166II GTNNAC 4 cut(s) 519, 608, 878, 1622
Hpy188I TCNGA 3 cut(s) 291, 493, 1143
Hpy188III TCNNGA 6 cut(s) 31, 433, 944, 953, 1149, 1476
Hpy8I GTNNAC 4 cut(s) 519, 608, 878, 1622
HpyAV CCTTC 5 cut(s) 580, 701, 1374, 1435, 1645
HpyCH4III ACNGT 3 cut(s) 554, 775, 1384
HpyCH4IV ACGT 3 cut(s) 1215, 1298, 1523
HpyF10VI GCNNNNNNNGC 4 cut(s) 312, 543, 1433, 1466
HpyF3I CTNAG 4 cut(s) 115, 167, 1065, 1142
HpySE526I ACGT 3 cut(s) 1215, 1298, 1523
Hsp92I GRCGYC 1 cut(s) 459
Hsp92II CATG 5 cut(s) 338, 947, 1174, 1498, 1545
KroI GCCGGC 1 cut(s) 455
KroNI GCCGGC 1 cut(s) 457
Ksp22I TGATCA 1 cut(s) 949
Kzo9I GATC 5 cut(s) 202, 717, 949, 1138, 1638
Lsp1109I GCAGC 5 cut(s) 182, 325, 376, 827, 1124
LweI GCATC 2 cut(s) 516, 1469
MabI ACCWGGT 1 cut(s) 624
MaeI CTAG 4 cut(s) 413, 1023, 1373, 1388
MaeII ACGT 3 cut(s) 1215, 1298, 1523
MaeIII GTNAC 8 cut(s) 460, 1005, 1121, 1162, 1202, 1216, 1397, 1524
MalI GATC 5 cut(s) 204, 719, 951, 1140, 1640
MboI GATC 5 cut(s) 202, 717, 949, 1138, 1638
MboII GAAGA 7 cut(s) 565, 658, 661, 1038, 1064, 1148, 1325
MflI RGATCY 2 cut(s) 202, 1138
MhlI GDGCHC 4 cut(s) 736, 1462, 1494, 1633
MmeI TCCRAC 1 cut(s) 942
MreI CGCCGGCG 1 cut(s) 455
MroNI GCCGGC 1 cut(s) 455
MseI TTAA 3 cut(s) 189, 1193, 1500
MslI CAYNNNNRTG 2 cut(s) 590, 1508
MspA1I CMGCKG 1 cut(s) 389
MspI CCGG 1 cut(s) 456
MspR9I CCNGG 3 cut(s) 232, 626, 1729
MvaI CCWGG 3 cut(s) 232, 626, 1729
MwoI GCNNNNNNNGC 4 cut(s) 312, 543, 1433, 1466
NaeI GCCGGC 1 cut(s) 457
NdeI CATATG 3 cut(s) 1072, 1339, 1564
NdeII GATC 5 cut(s) 202, 717, 949, 1138, 1638
NgoMIV GCCGGC 1 cut(s) 455
NlaIII CATG 5 cut(s) 338, 947, 1174, 1498, 1545
NlaIV GGNNCC 8 cut(s) 487, 568, 733, 1098, 1459, 1460, 1491, 1492
NmuCI GTSAC 3 cut(s) 460, 1216, 1524
OliI CACNNNNGTG 1 cut(s) 1508
PagI TCATGA 1 cut(s) 943
PcsI WCGNNNNNNNCGW 1 cut(s) 489
PdiI GCCGGC 1 cut(s) 457
PfeI GAWTC 1 cut(s) 1334
PflMI CCANNNNNTGG 1 cut(s) 887
PkrI GCNGC 5 cut(s) 197, 340, 391, 817, 1114
Ppu21I YACGTR 1 cut(s) 1216
PsiI TTATAA 1 cut(s) 225
Psp6I CCWGG 3 cut(s) 230, 624, 1727
PspGI CCWGG 3 cut(s) 230, 624, 1727
PspN4I GGNNCC 8 cut(s) 487, 568, 733, 1098, 1459, 1460, 1491, 1492
PspOMI GGGCCC 2 cut(s) 1458, 1490
PspPI GGNCC 5 cut(s) 486, 1458, 1459, 1490, 1491
PstI CTGCAG 2 cut(s) 308, 343
PsuI RGATCY 2 cut(s) 202, 1138
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 2 cut(s) 772, 868
RsaNI GTAC 2 cut(s) 771, 867
RseI CAYNNNNRTG 2 cut(s) 590, 1508
SaqAI TTAA 3 cut(s) 189, 1193, 1500
SatI GCNGC 5 cut(s) 196, 339, 390, 816, 1113
Sau3AI GATC 5 cut(s) 202, 717, 949, 1138, 1638
Sau96I GGNCC 5 cut(s) 486, 1458, 1459, 1490, 1491
ScrFI CCNGG 3 cut(s) 232, 626, 1729
SduI GDGCHC 4 cut(s) 736, 1462, 1494, 1633
SexAI ACCWGGT 1 cut(s) 624
SfaNI GCATC 2 cut(s) 516, 1469
SfcI CTRYAG 3 cut(s) 304, 339, 1086
SgrAI CRCCGGYG 1 cut(s) 455
SmiMI CAYNNNNRTG 2 cut(s) 590, 1508
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
SsiI CCGC 3 cut(s) 528, 860, 1487
SspI AATATT 2 cut(s) 163, 297
SspMI CTAG 4 cut(s) 413, 1023, 1373, 1388
StyD4I CCNGG 3 cut(s) 230, 624, 1727
StyI CCWWGG 2 cut(s) 475, 1259
TaaI ACNGT 3 cut(s) 554, 775, 1384
TaiI ACGT 3 cut(s) 1218, 1301, 1526
TfiI GAWTC 1 cut(s) 1334
Tru1I TTAA 3 cut(s) 189, 1193, 1500
Tru9I TTAA 3 cut(s) 189, 1193, 1500
TscAI CASTG 6 cut(s) 175, 559, 840, 1185, 1443, 1533
TseFI GTSAC 3 cut(s) 460, 1216, 1524
TseI GCWGC 5 cut(s) 195, 338, 389, 815, 1112
Tsp45I GTSAC 3 cut(s) 460, 1216, 1524
TspDTI ATGAA 3 cut(s) 37, 932, 1673
TspRI CASTG 6 cut(s) 175, 559, 840, 1185, 1443, 1533
Van91I CCANNNNNTGG 1 cut(s) 887
XapI RAATTY 1 cut(s) 925
XcmI CCANNNNNNNNNTGG 1 cut(s) 589
XmiI GTMKAC 2 cut(s) 607, 877
XspI CTAG 4 cut(s) 413, 1023, 1373, 1388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.