Rorug03G0279300

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000691
Physical Location & Seq
Forward (+)
27705477 .. 27707836
2360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0279300.1

Sequence Viewer

Length: 1017 bp
ATGACTGATTCCACCCTTATTGATTCTGAGACCGAGATCGAAGCCTCTCGAGGTAAAAATCAATTTGTTGTCTTCTTCTTTGATCAAGTTTTTGGTACCAGTAGAACAAGAGTGAATATGGCTGAGGGATCTTCCCAGGGGAAGAGGTTGCTGCAGCACCTTAAAGACCTACACGAGGAAAACAAGACACTCATGAAGCTCGTTGCAGACAAAGATAAGCGTATTGAATCGTACATGATAGATTTTGAAGAGCTGAATAGCTTGAAAATCATTGTCAATAAACTAGAGAAACAAAATTTCAAACTCACCCAAGCAAACAGCCAGATGTTGCAAGAGCTACGGAGTTTGAGAGACCATGTGCAGAAAGTACAACAACCAAATTTACCACCCGCCCAAGAAATAAACCAGGTGCAGGAAGTACAACAACCAAATTTGCAACCCCAAGAAATAATCCAGGTGCAGGAAGCACAACGACCAAATTTGCAACCTGCCCAAGCAAGAGTCCAGGTGCAGGAATTAGAACCACCAAATTTGCAAGCTGCCGAAGCAAGTAGCCAGATGCAGGAAGAAGAACCTGACCTAGAAAATAGCCAGATATTGCTGGAACTCAAATCACGTGATCATATGTTAAGTTCTACTGACGAAGCAGTCCGGGAGTGTATAGATATGATCGAAAAACTTCAAGCTTTGGGCCCTCCTACTAATGAAGCAATCCAACAAGTTAAGGCAAAGCTTCAAGCTTTGGGCCCTCTTACGATTGATGCAGTCCGGCAGATTAGAGTGGGGTTTCGTAAGCTTCAGAAATTGCAGTGTCCAAGGTGTGAATCTAATAACACTAAATTCAGTGTTTTGAAAGCCAAAAGAATATCTGCCCCAAGATATCACTGCAAAAATTGTAAAATGGAATGGACATTGGGGGGCGAAATAAGGAAACCTATTGTGCTTGGAGGCGTGAATAGAAGAGATCTTCTTCAGCAGCGCGTCCAACGTGTTCAGCGTAGGAGGATGAAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_Ca_ex

Protein Analysis

338

Amino Acids

38.93

Weight (kDa)

9.0

Isoelectric Point (pI)

60.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 268 - 321 8.3e-17 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 647
Acc36I ACCTGC 1 cut(s) 496
Acc65I GGTACC 1 cut(s) 95
AccB1I GGYRCC 1 cut(s) 95
AccII CGCG 1 cut(s) 981
AciI CCGC 1 cut(s) 390
AclWI GGATC 1 cut(s) 136
AcsI RAATTY 6 cut(s) 295, 379, 430, 478, 529, 839
AcuI CTGAAG 2 cut(s) 782, 956
AcvI CACGTG 1 cut(s) 617
AfaI GTAC 4 cut(s) 97, 233, 369, 420
AfiI CCNNNNNNNGG 5 cut(s) 175, 412, 460, 511, 562
AflIII ACRYGT 1 cut(s) 988
AgsI TTSAA 7 cut(s) 227, 248, 265, 301, 683, 737, 853
AjnI CCWGG 4 cut(s) 135, 405, 453, 504
AluBI AGCT 9 cut(s) 199, 253, 261, 337, 539, 686, 733, 740, 796
AluI AGCT 9 cut(s) 199, 253, 261, 337, 539, 686, 733, 740, 796
Alw26I GTCTC 2 cut(s) 23, 345
AlwI GGATC 1 cut(s) 136
Ama87I CYCGRG 1 cut(s) 48
AoxI GGCC 2 cut(s) 691, 745
ApaI GGGCCC 2 cut(s) 695, 749
ApeKI GCWGC 4 cut(s) 151, 154, 539, 976
ApoI RAATTY 6 cut(s) 295, 379, 430, 478, 529, 839
Asp700I GAANNNNTTC 1 cut(s) 678
Asp718I GGTACC 1 cut(s) 95
AspLEI GCGC 1 cut(s) 981
AspS9I GGNCC 4 cut(s) 691, 692, 745, 746
AsuC2I CCSGG 1 cut(s) 653
AsuHPI GGTGA 1 cut(s) 298
AvaI CYCGRG 1 cut(s) 48
BaeGI GKGCMC 2 cut(s) 695, 749
BanI GGYRCC 1 cut(s) 95
BanII GRGCYC 2 cut(s) 695, 749
BauI CACGAG 1 cut(s) 173
BbrPI CACGTG 1 cut(s) 617
BbsI GAAGAC 1 cut(s) 64
BbvCI CCTCAGC 1 cut(s) 123
BbvI GCAGC 4 cut(s) 138, 166, 526, 988
BciT130I CCWGG 4 cut(s) 137, 407, 455, 506
BclI TGATCA 2 cut(s) 82, 619
BcnI CCSGG 1 cut(s) 653
BcoDI GTCTC 2 cut(s) 23, 345
BfaI CTAG 2 cut(s) 284, 581
BfmI CTRYAG 1 cut(s) 152
BfuAI ACCTGC 1 cut(s) 496
BglII AGATCT 1 cut(s) 964
BisI GCNGC 4 cut(s) 152, 155, 540, 977
BlsI GCNGC 4 cut(s) 153, 156, 541, 978
Bme1390I CCNGG 5 cut(s) 137, 407, 455, 506, 653
BmeT110I CYCGRG 1 cut(s) 48
BmgT120I GGNCC 4 cut(s) 691, 692, 745, 746
BmiI GGNNCC 3 cut(s) 97, 693, 747
BmrFI CCNGG 5 cut(s) 137, 407, 455, 506, 653
BmsI GCATC 2 cut(s) 549, 751
BpiI GAAGAC 1 cut(s) 64
Bpu10I CCTNAGC 1 cut(s) 123
BpuMI CCSGG 1 cut(s) 653
BsaAI YACGTR 1 cut(s) 617
BsaI GGTCTC 2 cut(s) 23, 345
BsaJI CCNNGG 3 cut(s) 135, 136, 815
Bsc4I CCNNNNNNNGG 5 cut(s) 175, 412, 460, 511, 562
Bse1I ACTGG 1 cut(s) 99
BseBI CCWGG 4 cut(s) 137, 407, 455, 506
BseDI CCNNGG 3 cut(s) 135, 136, 815
BseGI GGATG 1 cut(s) 1011
BseLI CCNNNNNNNGG 5 cut(s) 175, 412, 460, 511, 562
BseMII CTCAG 2 cut(s) 18, 114
BseNI ACTGG 1 cut(s) 99
BseSI GKGCMC 2 cut(s) 695, 749
BseXI GCAGC 4 cut(s) 138, 166, 526, 988
BsgI GTGCAG 4 cut(s) 380, 431, 479, 530
Bsh1236I CGCG 1 cut(s) 981
BshFI GGCC 2 cut(s) 693, 747
BshNI GGYRCC 1 cut(s) 95
BsiHKCI CYCGRG 1 cut(s) 48
BsiSI CCGG 2 cut(s) 652, 769
BslI CCNNNNNNNGG 5 cut(s) 175, 412, 460, 511, 562
BsmAI GTCTC 2 cut(s) 23, 345
BsnI GGCC 2 cut(s) 693, 747
Bso31I GGTCTC 2 cut(s) 23, 345
BsoBI CYCGRG 1 cut(s) 48
Bsp120I GGGCCC 2 cut(s) 691, 745
Bsp1286I GDGCHC 2 cut(s) 695, 749
Bsp143I GATC 6 cut(s) 36, 82, 128, 619, 669, 964
BspACI CCGC 1 cut(s) 390
BspANI GGCC 2 cut(s) 693, 747
BspCNI CTCAG 2 cut(s) 19, 115
BspFNI CGCG 1 cut(s) 981
BspHI TCATGA 1 cut(s) 192
BspLI GGNNCC 3 cut(s) 97, 693, 747
BspMAI CTGCAG 1 cut(s) 156
BspMI ACCTGC 1 cut(s) 496
BspPI GGATC 1 cut(s) 136
BspQI GCTCTTC 1 cut(s) 243
BspT107I GGYRCC 1 cut(s) 95
BspTNI GGTCTC 2 cut(s) 23, 345
BsrI ACTGG 1 cut(s) 99
BssECI CCNNGG 3 cut(s) 135, 136, 815
BssMI GATC 6 cut(s) 36, 82, 128, 619, 669, 964
BssSI CACGAG 1 cut(s) 173
BssT1I CCWWGG 1 cut(s) 815
Bst2BI CACGAG 1 cut(s) 173
Bst2UI CCWGG 4 cut(s) 137, 407, 455, 506
Bst6I CTCTTC 3 cut(s) 137, 243, 955
BstBAI YACGTR 1 cut(s) 617
BstC8I GCNNGC 1 cut(s) 537
BstDEI CTNAG 2 cut(s) 27, 123
BstENI CCTNNNNNAGG 1 cut(s) 173
BstF5I GGATG 1 cut(s) 1011
BstFNI CGCG 1 cut(s) 981
BstHHI GCGC 1 cut(s) 981
BstKTI GATC 6 cut(s) 39, 85, 131, 622, 672, 967
BstMAI GTCTC 2 cut(s) 23, 345
BstMBI GATC 6 cut(s) 36, 82, 128, 619, 669, 964
BstMWI GCNNNNNNNGC 1 cut(s) 545
BstNI CCWGG 4 cut(s) 137, 407, 455, 506
BstSCI CCNGG 5 cut(s) 135, 405, 453, 504, 651
BstSFI CTRYAG 1 cut(s) 152
BstSLI GKGCMC 2 cut(s) 695, 749
BstUI CGCG 1 cut(s) 981
BstV1I GCAGC 4 cut(s) 138, 166, 526, 988
BstV2I GAAGAC 1 cut(s) 64
BstX2I RGATCY 2 cut(s) 128, 964
BstYI RGATCY 2 cut(s) 128, 964
BsuRI GGCC 2 cut(s) 693, 747
BtsCI GGATG 1 cut(s) 1011
BtsI GCAGTG 2 cut(s) 815, 883
BtsIMutI CAGTG 3 cut(s) 815, 850, 883
BveI ACCTGC 1 cut(s) 496
Cac8I GCNNGC 1 cut(s) 537
CciI TCATGA 1 cut(s) 192
CfoI GCGC 1 cut(s) 981
Cfr13I GGNCC 4 cut(s) 691, 692, 745, 746
CseI GACGC 1 cut(s) 970
CsiI ACCWGGT 1 cut(s) 405
Csp6I GTAC 4 cut(s) 96, 232, 368, 419
CspCI CAANNNNNGTGG 3 cut(s) 36, 513, 548
CviAII CATG 3 cut(s) 193, 235, 356
CviQI GTAC 4 cut(s) 96, 232, 368, 419
DdeI CTNAG 2 cut(s) 27, 123
DpnI GATC 6 cut(s) 38, 84, 130, 621, 671, 966
DpnII GATC 6 cut(s) 36, 82, 128, 619, 669, 964
DrdI GACNNNNNNGTC 1 cut(s) 647
DseDI GACNNNNNNGTC 1 cut(s) 647
Eam1104I CTCTTC 3 cut(s) 137, 243, 955
EarI CTCTTC 3 cut(s) 137, 243, 955
Eco130I CCWWGG 1 cut(s) 815
Eco24I GRGCYC 2 cut(s) 695, 749
Eco31I GGTCTC 2 cut(s) 23, 345
Eco32I GATATC 1 cut(s) 881
Eco57I CTGAAG 2 cut(s) 782, 956
Eco72I CACGTG 1 cut(s) 617
Eco88I CYCGRG 1 cut(s) 48
EcoNI CCTNNNNNAGG 1 cut(s) 173
EcoO109I RGGNCCY 2 cut(s) 692, 746
EcoRII CCWGG 4 cut(s) 135, 405, 453, 504
EcoRV GATATC 1 cut(s) 881
EcoT14I CCWWGG 1 cut(s) 815
EcoT38I GRGCYC 2 cut(s) 695, 749
ErhI CCWWGG 1 cut(s) 815
FaeI CATG 3 cut(s) 196, 238, 359
FaiI YATR 8 cut(s) 119, 194, 236, 357, 624, 626, 662, 668
FatI CATG 3 cut(s) 192, 234, 355
FauI CCCGC 1 cut(s) 397
FauNDI CATATG 1 cut(s) 624
FbaI TGATCA 2 cut(s) 82, 619
Fnu4HI GCNGC 4 cut(s) 152, 155, 540, 977
FriOI GRGCYC 2 cut(s) 695, 749
Fsp4HI GCNGC 4 cut(s) 152, 155, 540, 977
FspBI CTAG 2 cut(s) 284, 581
GlaI GCGC 1 cut(s) 980
GluI GCNGC 4 cut(s) 152, 155, 540, 977
HaeIII GGCC 2 cut(s) 693, 747
HapII CCGG 2 cut(s) 652, 769
HgaI GACGC 1 cut(s) 970
HhaI GCGC 1 cut(s) 981
Hin1II CATG 3 cut(s) 196, 238, 359
Hin6I GCGC 1 cut(s) 979
HinP1I GCGC 1 cut(s) 979
HindIII AAGCTT 4 cut(s) 684, 731, 738, 794
HinfI GANTC 5 cut(s) 8, 23, 227, 501, 824
HpaII CCGG 2 cut(s) 652, 769
HphI GGTGA 1 cut(s) 298
Hpy188I TCNGA 2 cut(s) 28, 801
Hpy188III TCNNGA 2 cut(s) 48, 193
HpyCH4IV ACGT 2 cut(s) 616, 988
HpyF10VI GCNNNNNNNGC 1 cut(s) 545
HpyF3I CTNAG 2 cut(s) 27, 123
HpySE526I ACGT 2 cut(s) 616, 988
Hsp92II CATG 3 cut(s) 196, 238, 359
HspAI GCGC 1 cut(s) 979
KpnI GGTACC 1 cut(s) 99
Ksp22I TGATCA 2 cut(s) 82, 619
Kzo9I GATC 6 cut(s) 36, 82, 128, 619, 669, 964
LguI GCTCTTC 1 cut(s) 243
Lsp1109I GCAGC 4 cut(s) 138, 166, 526, 988
LweI GCATC 2 cut(s) 549, 751
MabI ACCWGGT 1 cut(s) 405
MaeI CTAG 2 cut(s) 284, 581
MaeII ACGT 2 cut(s) 616, 988
MalI GATC 6 cut(s) 38, 84, 130, 621, 671, 966
MboI GATC 6 cut(s) 36, 82, 128, 619, 669, 964
MflI RGATCY 2 cut(s) 128, 964
MhlI GDGCHC 2 cut(s) 695, 749
MlyI GAGTC 1 cut(s) 510
MmeI TCCRAC 2 cut(s) 739, 1009
MnlI CCTC 9 cut(s) 44, 55, 118, 138, 169, 705, 759, 941, 996
MroXI GAANNNNTTC 1 cut(s) 678
MseI TTAA 3 cut(s) 162, 629, 723
MspI CCGG 2 cut(s) 652, 769
MspR9I CCNGG 5 cut(s) 137, 407, 455, 506, 653
MvaI CCWGG 4 cut(s) 137, 407, 455, 506
MvnI CGCG 1 cut(s) 981
MwoI GCNNNNNNNGC 1 cut(s) 545
NciI CCSGG 1 cut(s) 653
NdeI CATATG 1 cut(s) 624
NdeII GATC 6 cut(s) 36, 82, 128, 619, 669, 964
NlaIII CATG 3 cut(s) 196, 238, 359
NlaIV GGNNCC 3 cut(s) 97, 693, 747
PaeR7I CTCGAG 1 cut(s) 48
PagI TCATGA 1 cut(s) 192
PasI CCCWGGG 1 cut(s) 136
PciSI GCTCTTC 1 cut(s) 243
PcsI WCGNNNNNNNCGW 1 cut(s) 994
PdmI GAANNNNTTC 1 cut(s) 678
PfeI GAWTC 4 cut(s) 8, 23, 227, 824
PfoI TCCNGGA 1 cut(s) 651
PkrI GCNGC 4 cut(s) 153, 156, 541, 978
PleI GAGTC 1 cut(s) 509
PmaCI CACGTG 1 cut(s) 617
PmlI CACGTG 1 cut(s) 617
PpsI GAGTC 1 cut(s) 509
Ppu21I YACGTR 1 cut(s) 617
Psp6I CCWGG 4 cut(s) 135, 405, 453, 504
PspCI CACGTG 1 cut(s) 617
PspGI CCWGG 4 cut(s) 135, 405, 453, 504
PspN4I GGNNCC 3 cut(s) 97, 693, 747
PspOMI GGGCCC 2 cut(s) 691, 745
PspPI GGNCC 4 cut(s) 691, 692, 745, 746
PstI CTGCAG 1 cut(s) 156
PsuI RGATCY 2 cut(s) 128, 964
RsaI GTAC 4 cut(s) 97, 233, 369, 420
RsaNI GTAC 4 cut(s) 96, 232, 368, 419
SapI GCTCTTC 1 cut(s) 243
SaqAI TTAA 3 cut(s) 162, 629, 723
SatI GCNGC 4 cut(s) 152, 155, 540, 977
Sau3AI GATC 6 cut(s) 36, 82, 128, 619, 669, 964
Sau96I GGNCC 4 cut(s) 691, 692, 745, 746
SchI GAGTC 1 cut(s) 510
ScrFI CCNGG 5 cut(s) 137, 407, 455, 506, 653
SduI GDGCHC 2 cut(s) 695, 749
SexAI ACCWGGT 1 cut(s) 405
SfaNI GCATC 2 cut(s) 549, 751
SfcI CTRYAG 1 cut(s) 152
Sfr274I CTCGAG 1 cut(s) 48
SlaI CTCGAG 1 cut(s) 48
SmlI CTYRAG 1 cut(s) 48
SmoI CTYRAG 1 cut(s) 48
SsiI CCGC 1 cut(s) 390
SspMI CTAG 2 cut(s) 284, 581
StyD4I CCNGG 5 cut(s) 135, 405, 453, 504, 651
StyI CCWWGG 1 cut(s) 815
TaiI ACGT 2 cut(s) 619, 991
TaqI TCGA 3 cut(s) 39, 49, 672
TaqII GACCGA 1 cut(s) 47
TatI WGTACW 2 cut(s) 367, 418
TfiI GAWTC 4 cut(s) 8, 23, 227, 824
Tru1I TTAA 3 cut(s) 162, 629, 723
Tru9I TTAA 3 cut(s) 162, 629, 723
TscAI CASTG 3 cut(s) 815, 850, 890
TseI GCWGC 4 cut(s) 151, 154, 539, 976
TspDTI ATGAA 2 cut(s) 209, 720
TspGWI ACGGA 1 cut(s) 355
TspRI CASTG 3 cut(s) 815, 850, 890
XagI CCTNNNNNAGG 1 cut(s) 173
XapI RAATTY 6 cut(s) 295, 379, 430, 478, 529, 839
XhoI CTCGAG 1 cut(s) 48
XmnI GAANNNNTTC 1 cut(s) 678
XspI CTAG 2 cut(s) 284, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.