FvH4_6g31671

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000691
Physical Location & Seq
Forward (+)
24778112 .. 24782040
3929 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g31671.t1

Sequence Viewer

Length: 1767 bp
ATGACAGAGATTTCAAAGAAGTGGAGATATTTCAGACTCGATGGAGAGCCACCAACACCTCTTGATGTTAATTGGTTCACAATTGCATTTCACCATGGAGGGAGGTTTTACAAGTTGGGAAGTGGTAATAGATTTTACAAGGGAGGAGAGGTGGCTTATGTGGACAAAATGGAGATAGATACAATTTCATGGACTTGTCTGAACTGTTTTGTGGAAGACTTGGGCTACAGACAACCACCGATTATGTATTGGTTCAAGTTACCTGGAAGTGTAAATGGAGAAGGCTTATTGCCGATTAATGAAGACAAAGATGTTCAAGACATGCTGGGTGGTGTGAGAAGAAAGGACGAGGCAGAGCTAGAGGAGGCTGGAATTCTACCCAGTAAAGAAGTTAATCACCTACAGTATATGAGAAATATTGTAGATGACTTAGAAGAAGTGGAACCATTTTTAAATGGCAGTGGTGACGGAATATCTAATGAGTCAGAGATTACAAATGGATCAGAAAAGGGAAGTCAATCAAATGTGGTGAAAAACAAGGGTGGTAGGCCTAAAAATAAGAAGCACATGGAATTTACAAATGGATCAGAGAAGGGAAGTCAATCAAAGGTGGTGAAAAACAAGGGTGTGGTTATAGATAGTGACTGGGAACTTGATGATAAGGATGATGAGGTACTATTTGAAGCAAATGTTGATGGTGCCCTTGAAAATGTGATCGAGTACAATGACATGGGATATGTCATGGGATTTGCAGGTGATATATCTGATGGAGAAGGTGAAGATTCTGAAAAGTTTGACAGTGGGACTGATTCTACATCAAGTGATGATGAACGAGAAAGAGTTCTAAAAGGGGAGAGGAAGAAAAAGATGAGGTGGTCCAAGTGGAAGAAGTTCACGAAAGATGATATGAAGAACCTAATATTTAAGGTTGGTATGGAATTTCTGAATGTAACTGTTTTAAGGGATGCACTTAGGGTATTCGGAGTCAAGAATAAGAAGTCAATCAGGTTTAAGACCAATTATTCAAAAAGGGTCCAGGCGGTATGCAAAATAAATCCGGACTGTCCATTTCGGATATGGGTCTCTGTTACAGATAAACCGCCTACATTTGCCATCAGAACATTGAACCTAGAACCTGAATGCAGTGACTTAGAAGGGAAAGTATATCATTGCAATGCTCCTTTCATAGCTAAACATTATCTTGATACTTTCATTGCTGATAAGAATTGGTCAAAGGAAGACATACAATCTATAGTGGCAAGGGATTATGGTTTACATGTTGGGTTTCAAACGTGTTACAGGGCCAGAAGGAGAGCTGGAGAAATGGCAGAAGCCATAAACCCAATTCCTGGTGTTGAAGAATGTGAGGTTATTGAAAAGCCAATCCTGCCACCAGCATACATAAGAGGACCTGGAAGGCTGCCTAAACTCTCAAGAAACATAGAACCAGATGAGCAACATCGACCACCTCCTGGAACTACTAGACCAGCTCTTGGAATAGAAAAGTTACCAATGACTTACTATCAAGAAATTACATGTGGAAGATGTAACAAGAAAGGGCATAATAGCAGAAGCTGTGAAAGAAGAGAAGCACAGTCCCAAACAAAAGTGGAATCATCATCTTCTCAAAACCAAGGAAATGGTGTGCATGTTGAACGGACTTCAAATGCTGACAATGTTCAACCGAATACCTGTGTGAGAACTCAACGCAACAAAGCACCCACATCAAATGAAAAATCTCACTACAAGCCAAATTGGAAGCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

589

Amino Acids

66.78

Weight (kDa)

6.64

Isoelectric Point (pI)

45.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1-like PF26130 25 - 117 3.8e-18 PB1-like domain
DBD_Tnp_Mut PF03108 306 - 372 2.1e-10 MuDR family transposase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 743
Acc36I ACCTGC 1 cut(s) 743
AccB1I GGYRCC 1 cut(s) 698
AccB7I CCANNNNNTGG 3 cut(s) 1349, 1472, 1493
AccIII TCCGGA 1 cut(s) 1057
AciI CCGC 2 cut(s) 1040, 1100
AclWI GGATC 2 cut(s) 508, 592
AcsI RAATTY 3 cut(s) 372, 572, 938
AfaI GTAC 2 cut(s) 675, 722
AfiI CCNNNNNNNGG 3 cut(s) 1349, 1472, 1493
AflIII ACRYGT 3 cut(s) 1276, 1292, 1535
AjnI CCWGG 5 cut(s) 262, 1035, 1348, 1411, 1471
AluBI AGCT 5 cut(s) 358, 1190, 1316, 1490, 1575
AluI AGCT 5 cut(s) 358, 1190, 1316, 1490, 1575
Alw26I GTCTC 1 cut(s) 1087
AlwI GGATC 2 cut(s) 508, 592
AlwNI CAGNNNCTG 1 cut(s) 1575
Aor13HI TCCGGA 1 cut(s) 1057
AoxI GGCC 2 cut(s) 548, 1302
ApeKI GCWGC 1 cut(s) 1420
ApoI RAATTY 3 cut(s) 372, 572, 938
AseI ATTAAT 1 cut(s) 297
Asp700I GAANNNNTTC 2 cut(s) 840, 890
AspS9I GGNCC 4 cut(s) 876, 1033, 1302, 1409
AsuHPI GGTGA 7 cut(s) 83, 389, 476, 541, 625, 767, 788
AvaII GGWCC 3 cut(s) 876, 1033, 1409
BaeGI GKGCMC 1 cut(s) 703
BanI GGYRCC 1 cut(s) 698
BbsI GAAGAC 3 cut(s) 222, 309, 1245
BbvI GCAGC 1 cut(s) 1407
BccI CCATC 4 cut(s) 35, 689, 761, 1121
BceAI ACGGC 1 cut(s) 1747
BciT130I CCWGG 5 cut(s) 264, 1037, 1350, 1413, 1473
BcoDI GTCTC 1 cut(s) 1087
BfaI CTAG 3 cut(s) 359, 1130, 1482
BfmI CTRYAG 3 cut(s) 226, 401, 1251
BfuAI ACCTGC 1 cut(s) 743
BisI GCNGC 1 cut(s) 1421
BlsI GCNGC 1 cut(s) 1422
Bme1390I CCNGG 5 cut(s) 264, 1037, 1350, 1413, 1473
Bme18I GGWCC 3 cut(s) 876, 1033, 1409
BmgT120I GGNCC 4 cut(s) 876, 1033, 1302, 1409
BmiI GGNNCC 3 cut(s) 444, 700, 1034
BmrFI CCNGG 5 cut(s) 264, 1037, 1350, 1413, 1473
BmrI ACTGGG 2 cut(s) 375, 655
BmsI GCATC 1 cut(s) 955
BmuI ACTGGG 2 cut(s) 375, 655
BpiI GAAGAC 3 cut(s) 222, 309, 1245
BpmI CTGGAG 1 cut(s) 1338
BpuEI CTTGAG 1 cut(s) 1417
BsaI GGTCTC 1 cut(s) 1087
BsaJI CCNNGG 2 cut(s) 94, 1633
BsaWI WCCGGW 1 cut(s) 1057
BsaXI ACNNNNNCTCC 2 cut(s) 135, 165
Bsc4I CCNNNNNNNGG 3 cut(s) 1349, 1472, 1493
Bse1I ACTGG 2 cut(s) 381, 650
Bse3DI GCAATG 3 cut(s) 1168, 1180, 1212
BseAI TCCGGA 1 cut(s) 1057
BseBI CCWGG 5 cut(s) 264, 1037, 1350, 1413, 1473
BseDI CCNNGG 2 cut(s) 94, 1633
BseGI GGATG 2 cut(s) 670, 970
BseLI CCNNNNNNNGG 3 cut(s) 1349, 1472, 1493
BseMI GCAATG 3 cut(s) 1168, 1180, 1212
BseNI ACTGG 2 cut(s) 381, 650
BseRI GAGGAG 2 cut(s) 159, 377
BseSI GKGCMC 1 cut(s) 703
BseXI GCAGC 1 cut(s) 1407
BseYI CCCAGC 1 cut(s) 325
BshFI GGCC 2 cut(s) 550, 1304
BshNI GGYRCC 1 cut(s) 698
BsiSI CCGG 1 cut(s) 1058
BslFI GGGAC 2 cut(s) 817, 1582
BslI CCNNNNNNNGG 3 cut(s) 1349, 1472, 1493
BsmAI GTCTC 1 cut(s) 1087
BsmFI GGGAC 2 cut(s) 817, 1582
BsmI GAATGC 1 cut(s) 1145
BsnI GGCC 2 cut(s) 550, 1304
Bso31I GGTCTC 1 cut(s) 1087
Bsp1286I GDGCHC 1 cut(s) 703
Bsp13I TCCGGA 1 cut(s) 1057
Bsp143I GATC 3 cut(s) 500, 584, 714
Bsp19I CCATGG 1 cut(s) 94
BspACI CCGC 2 cut(s) 1040, 1100
BspANI GGCC 2 cut(s) 550, 1304
BspEI TCCGGA 1 cut(s) 1057
BspLI GGNNCC 3 cut(s) 444, 700, 1034
BspMI ACCTGC 1 cut(s) 743
BspPI GGATC 2 cut(s) 508, 592
BspT107I GGYRCC 1 cut(s) 698
BspTNI GGTCTC 1 cut(s) 1087
BsrDI GCAATG 3 cut(s) 1168, 1180, 1212
BsrI ACTGG 2 cut(s) 381, 650
BssECI CCNNGG 2 cut(s) 94, 1633
BssMI GATC 3 cut(s) 500, 584, 714
BssT1I CCWWGG 2 cut(s) 94, 1633
Bst2UI CCWGG 5 cut(s) 264, 1037, 1350, 1413, 1473
Bst4CI ACNGT 6 cut(s) 206, 405, 800, 955, 1064, 1596
Bst6I CTCTTC 1 cut(s) 1579
BstDEI CTNAG 3 cut(s) 430, 971, 1150
BstDSI CCRYGG 1 cut(s) 94
BstF5I GGATG 2 cut(s) 670, 970
BstKTI GATC 3 cut(s) 503, 587, 717
BstMAI GTCTC 1 cut(s) 1087
BstMBI GATC 3 cut(s) 500, 584, 714
BstMWI GCNNNNNNNGC 1 cut(s) 1387
BstNI CCWGG 5 cut(s) 264, 1037, 1350, 1413, 1473
BstNSI RCATGY 4 cut(s) 325, 1280, 1539, 1652
BstSCI CCNGG 5 cut(s) 262, 1035, 1348, 1411, 1471
BstSFI CTRYAG 3 cut(s) 226, 401, 1251
BstSLI GKGCMC 1 cut(s) 703
BstV1I GCAGC 1 cut(s) 1407
BstV2I GAAGAC 3 cut(s) 222, 309, 1245
BstXI CCANNNNNNTGG 1 cut(s) 1640
BsuRI GGCC 2 cut(s) 550, 1304
BtgI CCRYGG 1 cut(s) 94
BtsCI GGATG 2 cut(s) 670, 970
BtsI GCAGTG 2 cut(s) 466, 1150
BtsIMutI CAGTG 3 cut(s) 466, 805, 1150
BveI ACCTGC 1 cut(s) 743
CaiI CAGNNNCTG 1 cut(s) 1575
Cfr13I GGNCC 4 cut(s) 876, 1033, 1302, 1409
Csp6I GTAC 2 cut(s) 674, 721
CviAII CATG 9 cut(s) 95, 189, 322, 568, 730, 742, 1277, 1536, 1649
CviQI GTAC 2 cut(s) 674, 721
DdeI CTNAG 3 cut(s) 430, 971, 1150
DpnI GATC 3 cut(s) 502, 586, 716
DpnII GATC 3 cut(s) 500, 584, 714
DraI TTTAAA 1 cut(s) 453
Eam1104I CTCTTC 1 cut(s) 1579
EarI CTCTTC 1 cut(s) 1579
Eco130I CCWWGG 2 cut(s) 94, 1633
Eco147I AGGCCT 1 cut(s) 550
Eco31I GGTCTC 1 cut(s) 1087
Eco47I GGWCC 3 cut(s) 876, 1033, 1409
EcoO109I RGGNCCY 1 cut(s) 1409
EcoRI GAATTC 1 cut(s) 372
EcoRII CCWGG 5 cut(s) 262, 1035, 1348, 1411, 1471
EcoT14I CCWWGG 2 cut(s) 94, 1633
ErhI CCWWGG 2 cut(s) 94, 1633
FaeI CATG 9 cut(s) 98, 192, 325, 571, 733, 745, 1280, 1539, 1652
FaqI GGGAC 2 cut(s) 817, 1582
FatI CATG 9 cut(s) 94, 188, 321, 567, 729, 741, 1276, 1535, 1648
Fnu4HI GCNGC 1 cut(s) 1421
FokI GGATG 2 cut(s) 677, 977
Fsp4HI GCNGC 1 cut(s) 1421
FspBI CTAG 3 cut(s) 359, 1130, 1482
GluI GCNGC 1 cut(s) 1421
GsaI CCCAGC 1 cut(s) 329
GsuI CTGGAG 1 cut(s) 1338
HaeIII GGCC 2 cut(s) 550, 1304
HapII CCGG 1 cut(s) 1058
Hin1II CATG 9 cut(s) 98, 192, 325, 571, 733, 745, 1280, 1539, 1652
HinfI GANTC 6 cut(s) 36, 482, 782, 809, 984, 1613
HpaII CCGG 1 cut(s) 1058
HphI GGTGA 7 cut(s) 83, 389, 476, 541, 625, 767, 788
Hpy166II GTNNAC 4 cut(s) 78, 163, 894, 1274
Hpy188III TCNNGA 8 cut(s) 62, 317, 895, 988, 1058, 1202, 1434, 1526
Hpy8I GTNNAC 4 cut(s) 78, 163, 894, 1274
HpyAV CCTTC 6 cut(s) 275, 586, 767, 1148, 1302, 1410
HpyCH4III ACNGT 6 cut(s) 206, 405, 800, 955, 1064, 1596
HpyCH4IV ACGT 1 cut(s) 1292
HpyCH4V TGCA 7 cut(s) 86, 752, 968, 1047, 1143, 1173, 1648
HpyF10VI GCNNNNNNNGC 1 cut(s) 1387
HpyF3I CTNAG 3 cut(s) 430, 971, 1150
HpySE526I ACGT 1 cut(s) 1292
Hsp92II CATG 9 cut(s) 98, 192, 325, 571, 733, 745, 1280, 1539, 1652
Kpn2I TCCGGA 1 cut(s) 1057
Kzo9I GATC 3 cut(s) 500, 584, 714
LmnI GCTCC 1 cut(s) 1183
Lsp1109I GCAGC 1 cut(s) 1407
LweI GCATC 1 cut(s) 955
MaeI CTAG 3 cut(s) 359, 1130, 1482
MaeII ACGT 1 cut(s) 1292
MaeIII GTNAC 9 cut(s) 258, 464, 641, 949, 1087, 1145, 1295, 1506, 1547
MalI GATC 3 cut(s) 502, 586, 716
MboI GATC 3 cut(s) 500, 584, 714
MfeI CAATTG 1 cut(s) 81
MhlI GDGCHC 1 cut(s) 703
MlyI GAGTC 3 cut(s) 30, 491, 993
MroI TCCGGA 1 cut(s) 1057
MroXI GAANNNNTTC 2 cut(s) 840, 890
MseI TTAA 7 cut(s) 69, 297, 393, 452, 924, 959, 1011
MslI CAYNNNNRTG 1 cut(s) 1173
MspI CCGG 1 cut(s) 1058
MspR9I CCNGG 5 cut(s) 264, 1037, 1350, 1413, 1473
MunI CAATTG 1 cut(s) 81
Mva1269I GAATGC 1 cut(s) 1145
MvaI CCWGG 5 cut(s) 264, 1037, 1350, 1413, 1473
MwoI GCNNNNNNNGC 1 cut(s) 1387
NcoI CCATGG 1 cut(s) 94
NdeII GATC 3 cut(s) 500, 584, 714
NlaIII CATG 9 cut(s) 98, 192, 325, 571, 733, 745, 1280, 1539, 1652
NlaIV GGNNCC 3 cut(s) 444, 700, 1034
NmuCI GTSAC 3 cut(s) 464, 641, 1145
NspI RCATGY 4 cut(s) 325, 1280, 1539, 1652
PaqCI CACCTGC 1 cut(s) 743
PceI AGGCCT 1 cut(s) 550
PciI ACATGT 2 cut(s) 1276, 1535
PctI GAATGC 1 cut(s) 1145
PdmI GAANNNNTTC 2 cut(s) 840, 890
PfeI GAWTC 3 cut(s) 782, 809, 1613
PflMI CCANNNNNTGG 3 cut(s) 1349, 1472, 1493
PfoI TCCNGGA 1 cut(s) 1471
PkrI GCNGC 1 cut(s) 1422
PleI GAGTC 3 cut(s) 30, 490, 992
PpsI GAGTC 3 cut(s) 30, 490, 992
PpuMI RGGWCCY 1 cut(s) 1409
PscI ACATGT 2 cut(s) 1276, 1535
PshBI ATTAAT 1 cut(s) 297
Psp5II RGGWCCY 1 cut(s) 1409
Psp6I CCWGG 5 cut(s) 262, 1035, 1348, 1411, 1471
PspFI CCCAGC 1 cut(s) 325
PspGI CCWGG 5 cut(s) 262, 1035, 1348, 1411, 1471
PspN4I GGNNCC 3 cut(s) 444, 700, 1034
PspPI GGNCC 4 cut(s) 876, 1033, 1302, 1409
PspPPI RGGWCCY 1 cut(s) 1409
PstNI CAGNNNCTG 1 cut(s) 1575
RsaI GTAC 2 cut(s) 675, 722
RsaNI GTAC 2 cut(s) 674, 721
RseI CAYNNNNRTG 1 cut(s) 1173
SaqAI TTAA 7 cut(s) 69, 297, 393, 452, 924, 959, 1011
SatI GCNGC 1 cut(s) 1421
Sau3AI GATC 3 cut(s) 500, 584, 714
Sau96I GGNCC 4 cut(s) 876, 1033, 1302, 1409
SchI GAGTC 3 cut(s) 30, 491, 993
ScrFI CCNGG 5 cut(s) 264, 1037, 1350, 1413, 1473
SduI GDGCHC 1 cut(s) 703
SfaNI GCATC 1 cut(s) 955
SfcI CTRYAG 3 cut(s) 226, 401, 1251
SinI GGWCC 3 cut(s) 876, 1033, 1409
SmiMI CAYNNNNRTG 1 cut(s) 1173
SmlI CTYRAG 1 cut(s) 1432
SmoI CTYRAG 1 cut(s) 1432
SseBI AGGCCT 1 cut(s) 550
SsiI CCGC 2 cut(s) 1040, 1100
SspI AATATT 2 cut(s) 418, 921
SspMI CTAG 3 cut(s) 359, 1130, 1482
StuI AGGCCT 1 cut(s) 550
StyD4I CCNGG 5 cut(s) 262, 1035, 1348, 1411, 1471
StyI CCWWGG 2 cut(s) 94, 1633
TaaI ACNGT 6 cut(s) 206, 405, 800, 955, 1064, 1596
TaiI ACGT 1 cut(s) 1295
TaqI TCGA 3 cut(s) 39, 717, 1462
TatI WGTACW 1 cut(s) 720
TfiI GAWTC 3 cut(s) 782, 809, 1613
Tru1I TTAA 7 cut(s) 69, 297, 393, 452, 924, 959, 1011
Tru9I TTAA 7 cut(s) 69, 297, 393, 452, 924, 959, 1011
TscAI CASTG 3 cut(s) 466, 805, 1150
TseFI GTSAC 3 cut(s) 464, 641, 1145
TseI GCWGC 1 cut(s) 1420
Tsp45I GTSAC 3 cut(s) 464, 641, 1145
TspDTI ATGAA 7 cut(s) 177, 315, 843, 923, 1174, 1201, 1746
TspGWI ACGGA 2 cut(s) 483, 1672
TspRI CASTG 3 cut(s) 466, 805, 1150
Van91I CCANNNNNTGG 3 cut(s) 1349, 1472, 1493
VpaK11BI GGWCC 3 cut(s) 876, 1033, 1409
VspI ATTAAT 1 cut(s) 297
XapI RAATTY 3 cut(s) 372, 572, 938
XceI RCATGY 4 cut(s) 325, 1280, 1539, 1652
XcmI CCANNNNNNNNNTGG 1 cut(s) 1074
XmnI GAANNNNTTC 2 cut(s) 840, 890
XspI CTAG 3 cut(s) 359, 1130, 1482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.