Rh6CG449000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000691
Physical Location & Seq
Forward (+)
62686368 .. 62698389
12022 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG449000.1

Sequence Viewer

Length: 639 bp
ATGATCGAAGAGAAACAAACTTTTCACAGTCCAAAATATGAATTAAACGGTAATTTAAAATTTGCAACTACATATAATTTTTTGTGCCAGACGCGCGGCGCTACTATAAATTTCTGTGATGGAAACTGGTCCAATCAGTTTACATTGGTCGGTATGGTAAATGACCATTTAGTCCCCGGCTTGCATGGGATTGGTTTGGTGGCAATACGGCGGTGCATCTGCTCTGAGAAATTTGCAGTCGCACAGCAAAGCGATGCAAATGAAAAAGGTTCTCAAAATGTTCAGCAAGATGGCAATGCAAATGATGAACATAGAATGCAGAATGCTCAGCAATGTGGCAATTCTAATGGACATAGTTCTGAAAATGTTCACGATGAGGAGCATCATGAGATTCCATCTCAACAATCACTTGTGAAAGCACCTTTTGTTTCATCGCAACCACTTCCATCACCTCTAAATTTCTCACAAGAAATTCCTCCAGTTCAAAGAAGTTCTCAACCTATTGGATCATTCGTAGCTTCCTTCTCCGTGATGTACATTGATCCTATAACAGGGAGGCCAAAGAAGCAAGTTGCATTCAGAAATCCTAAGAATTCTATGTCAACTGAGAATGCAAGCAAGCCTATTTGGAGACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

212

Amino Acids

23.57

Weight (kDa)

6.7

Isoelectric Point (pI)

50.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 170
AccII CGCG 2 cut(s) 94, 96
AciI CCGC 2 cut(s) 96, 211
AclWI GGATC 2 cut(s) 514, 536
AcsI RAATTY 6 cut(s) 59, 109, 230, 457, 471, 592
AfaI GTAC 1 cut(s) 536
AfiI CCNNNNNNNGG 1 cut(s) 551
AgsI TTSAA 1 cut(s) 485
AluBI AGCT 1 cut(s) 518
AluI AGCT 1 cut(s) 518
Alw26I GTCTC 1 cut(s) 625
AlwI GGATC 2 cut(s) 514, 536
AoxI GGCC 1 cut(s) 557
ApoI RAATTY 6 cut(s) 59, 109, 230, 457, 471, 592
Asp700I GAANNNNTTC 1 cut(s) 366
AspLEI GCGC 2 cut(s) 96, 101
AspS9I GGNCC 1 cut(s) 129
AsuC2I CCSGG 1 cut(s) 177
AsuHPI GGTGA 1 cut(s) 441
AvaII GGWCC 1 cut(s) 129
BccI CCATC 4 cut(s) 113, 284, 403, 454
BceAI ACGGC 1 cut(s) 224
BcnI CCSGG 1 cut(s) 177
BcoDI GTCTC 1 cut(s) 625
BfoI RGCGCY 1 cut(s) 102
BisI GCNGC 1 cut(s) 97
BlpI GCTNAGC 1 cut(s) 327
BlsI GCNGC 1 cut(s) 98
Bme1390I CCNGG 1 cut(s) 177
Bme18I GGWCC 1 cut(s) 129
BmgT120I GGNCC 1 cut(s) 129
BmrFI CCNGG 1 cut(s) 177
BmsI GCATC 3 cut(s) 225, 244, 391
BpmI CTGGAG 1 cut(s) 462
Bpu1102I GCTNAGC 1 cut(s) 327
BpuMI CCSGG 1 cut(s) 177
BsaI GGTCTC 1 cut(s) 625
BsaJI CCNNGG 1 cut(s) 175
Bsc4I CCNNNNNNNGG 1 cut(s) 551
Bse1I ACTGG 2 cut(s) 131, 479
Bse3DI GCAATG 2 cut(s) 301, 338
BseDI CCNNGG 1 cut(s) 175
BseLI CCNNNNNNNGG 1 cut(s) 551
BseMI GCAATG 2 cut(s) 301, 338
BseMII CTCAG 3 cut(s) 216, 341, 597
BseNI ACTGG 2 cut(s) 131, 479
BseRI GAGGAG 1 cut(s) 392
Bsh1236I CGCG 2 cut(s) 94, 96
BshFI GGCC 1 cut(s) 559
BsiSI CCGG 1 cut(s) 177
BslFI GGGAC 1 cut(s) 158
BslI CCNNNNNNNGG 1 cut(s) 551
BsmAI GTCTC 1 cut(s) 625
BsmFI GGGAC 1 cut(s) 158
BsmI GAATGC 4 cut(s) 321, 328, 575, 616
BsnI GGCC 1 cut(s) 559
Bso31I GGTCTC 1 cut(s) 625
Bsp1407I TGTACA 1 cut(s) 534
Bsp143I GATC 3 cut(s) 3, 506, 541
Bsp1720I GCTNAGC 1 cut(s) 327
BspACI CCGC 2 cut(s) 96, 211
BspANI GGCC 1 cut(s) 559
BspCNI CTCAG 3 cut(s) 217, 340, 598
BspFNI CGCG 2 cut(s) 94, 96
BspHI TCATGA 1 cut(s) 385
BspPI GGATC 2 cut(s) 514, 536
BspTNI GGTCTC 1 cut(s) 625
BsrDI GCAATG 2 cut(s) 301, 338
BsrGI TGTACA 1 cut(s) 534
BsrI ACTGG 2 cut(s) 131, 479
BssECI CCNNGG 1 cut(s) 175
BssMI GATC 3 cut(s) 3, 506, 541
Bst4CI ACNGT 2 cut(s) 29, 50
Bst6I CTCTTC 1 cut(s) 3
BstAUI TGTACA 1 cut(s) 534
BstC8I GCNNGC 3 cut(s) 182, 616, 620
BstDEI CTNAG 4 cut(s) 225, 327, 588, 606
BstENI CCTNNNNNAGG 1 cut(s) 549
BstFNI CGCG 2 cut(s) 94, 96
BstH2I RGCGCY 1 cut(s) 102
BstHHI GCGC 2 cut(s) 96, 101
BstKTI GATC 3 cut(s) 6, 509, 544
BstMAI GTCTC 1 cut(s) 625
BstMBI GATC 3 cut(s) 3, 506, 541
BstMWI GCNNNNNNNGC 2 cut(s) 93, 565
BstSCI CCNGG 1 cut(s) 175
BstUI CGCG 2 cut(s) 94, 96
BsuRI GGCC 1 cut(s) 559
BtgZI GCGATG 2 cut(s) 267, 417
Cac8I GCNNGC 3 cut(s) 182, 616, 620
CciI TCATGA 1 cut(s) 385
CfoI GCGC 2 cut(s) 96, 101
Cfr13I GGNCC 1 cut(s) 129
CseI GACGC 1 cut(s) 100
Csp6I GTAC 1 cut(s) 535
CviAII CATG 2 cut(s) 185, 386
CviJI RGCY 4 cut(s) 180, 518, 559, 622
CviKI_1 RGCY 4 cut(s) 180, 518, 559, 622
CviQI GTAC 1 cut(s) 535
DdeI CTNAG 4 cut(s) 225, 327, 588, 606
DpnI GATC 3 cut(s) 5, 508, 543
DpnII GATC 3 cut(s) 3, 506, 541
DraI TTTAAA 1 cut(s) 57
DrdI GACNNNNNNGTC 1 cut(s) 170
DseDI GACNNNNNNGTC 1 cut(s) 170
Eam1104I CTCTTC 1 cut(s) 3
EarI CTCTTC 1 cut(s) 3
Eco31I GGTCTC 1 cut(s) 625
Eco47I GGWCC 1 cut(s) 129
EcoNI CCTNNNNNAGG 1 cut(s) 549
EcoRI GAATTC 1 cut(s) 592
FaeI CATG 2 cut(s) 188, 389
FaqI GGGAC 1 cut(s) 158
FatI CATG 2 cut(s) 184, 385
Fnu4HI GCNGC 1 cut(s) 97
Fsp4HI GCNGC 1 cut(s) 97
GlaI GCGC 2 cut(s) 95, 100
GluI GCNGC 1 cut(s) 97
GsuI CTGGAG 1 cut(s) 462
HaeII RGCGCY 1 cut(s) 102
HaeIII GGCC 1 cut(s) 559
HapII CCGG 1 cut(s) 177
HgaI GACGC 1 cut(s) 100
HhaI GCGC 2 cut(s) 96, 101
Hin1II CATG 2 cut(s) 188, 389
Hin6I GCGC 2 cut(s) 94, 99
HinP1I GCGC 2 cut(s) 94, 99
HincII GTYRAC 1 cut(s) 603
HindII GTYRAC 1 cut(s) 603
HinfI GANTC 1 cut(s) 391
HpaII CCGG 1 cut(s) 177
HphI GGTGA 1 cut(s) 441
Hpy166II GTNNAC 3 cut(s) 141, 370, 603
Hpy188I TCNGA 3 cut(s) 226, 361, 581
Hpy188III TCNNGA 2 cut(s) 371, 386
Hpy8I GTNNAC 3 cut(s) 141, 370, 603
HpyAV CCTTC 1 cut(s) 532
HpyCH4III ACNGT 2 cut(s) 29, 50
HpyCH4V TGCA 9 cut(s) 65, 184, 216, 236, 257, 299, 319, 575, 614
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 565
HpyF3I CTNAG 4 cut(s) 225, 327, 588, 606
Hsp92II CATG 2 cut(s) 188, 389
HspAI GCGC 2 cut(s) 94, 99
Kzo9I GATC 3 cut(s) 3, 506, 541
LmnI GCTCC 1 cut(s) 379
LpnPI CCDG 5 cut(s) 101, 112, 190, 492, 537
LweI GCATC 3 cut(s) 225, 244, 391
MalI GATC 3 cut(s) 5, 508, 543
MboI GATC 3 cut(s) 3, 506, 541
MboII GAAGA 1 cut(s) 20
MnlI CCTC 4 cut(s) 370, 462, 486, 549
MroXI GAANNNNTTC 1 cut(s) 366
MseI TTAA 3 cut(s) 44, 56, 637
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 1 cut(s) 177
MteI GCGCNGCGC 1 cut(s) 97
Mva1269I GAATGC 4 cut(s) 321, 328, 575, 616
MvnI CGCG 2 cut(s) 94, 96
MwoI GCNNNNNNNGC 2 cut(s) 93, 565
NciI CCSGG 1 cut(s) 177
NdeII GATC 3 cut(s) 3, 506, 541
NlaIII CATG 2 cut(s) 188, 389
PagI TCATGA 1 cut(s) 385
PctI GAATGC 4 cut(s) 321, 328, 575, 616
PdmI GAANNNNTTC 1 cut(s) 366
PfeI GAWTC 1 cut(s) 391
PkrI GCNGC 1 cut(s) 98
PspPI GGNCC 1 cut(s) 129
RsaI GTAC 1 cut(s) 536
RsaNI GTAC 1 cut(s) 535
SaqAI TTAA 3 cut(s) 44, 56, 637
SatI GCNGC 1 cut(s) 97
Sau3AI GATC 3 cut(s) 3, 506, 541
Sau96I GGNCC 1 cut(s) 129
ScrFI CCNGG 1 cut(s) 177
SetI ASST 6 cut(s) 271, 424, 454, 502, 520, 637
SfaNI GCATC 3 cut(s) 225, 244, 391
SinI GGWCC 1 cut(s) 129
SsiI CCGC 2 cut(s) 96, 211
StyD4I CCNGG 1 cut(s) 175
TaaI ACNGT 2 cut(s) 29, 50
TaqI TCGA 1 cut(s) 6
TatI WGTACW 1 cut(s) 534
TauI GCSGC 1 cut(s) 99
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 3 cut(s) 44, 56, 637
Tru9I TTAA 3 cut(s) 44, 56, 637
TspDTI ATGAA 4 cut(s) 54, 276, 321, 420
TspGWI ACGGA 1 cut(s) 517
VpaK11BI GGWCC 1 cut(s) 129
XagI CCTNNNNNAGG 1 cut(s) 549
XapI RAATTY 6 cut(s) 59, 109, 230, 457, 471, 592
XmnI GAANNNNTTC 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.