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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 2 cut(s) | 274, 299 |
| AcsI | RAATTY | 3 cut(s) | 217, 231, 355 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 314 |
| AgsI | TTSAA | 1 cut(s) | 245 |
| AluBI | AGCT | 1 cut(s) | 278 |
| AluI | AGCT | 1 cut(s) | 278 |
| AlwI | GGATC | 2 cut(s) | 274, 299 |
| AoxI | GGCC | 1 cut(s) | 320 |
| ApeKI | GCWGC | 1 cut(s) | 4 |
| ApoI | RAATTY | 3 cut(s) | 217, 231, 355 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 126 |
| AspLEI | GCGC | 1 cut(s) | 181 |
| AsuHPI | GGTGA | 1 cut(s) | 201 |
| BbvI | GCAGC | 1 cut(s) | 16 |
| BccI | CCATC | 4 cut(s) | 44, 163, 214, 299 |
| BfmI | CTRYAG | 1 cut(s) | 387 |
| BfoI | RGCGCY | 1 cut(s) | 182 |
| BisI | GCNGC | 1 cut(s) | 5 |
| BlpI | GCTNAGC | 1 cut(s) | 87 |
| BlsI | GCNGC | 1 cut(s) | 6 |
| BmsI | GCATC | 1 cut(s) | 151 |
| BpmI | CTGGAG | 1 cut(s) | 222 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 87 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 314 |
| Bse1I | ACTGG | 1 cut(s) | 239 |
| Bse3DI | GCAATG | 3 cut(s) | 19, 61, 98 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 314 |
| BseMI | GCAATG | 3 cut(s) | 19, 61, 98 |
| BseMII | CTCAG | 2 cut(s) | 101, 360 |
| BseNI | ACTGG | 1 cut(s) | 239 |
| BseRI | GAGGAG | 2 cut(s) | 152, 277 |
| BseXI | GCAGC | 1 cut(s) | 16 |
| BshFI | GGCC | 1 cut(s) | 322 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 314 |
| BsmI | GAATGC | 4 cut(s) | 81, 88, 338, 379 |
| BsnI | GGCC | 1 cut(s) | 322 |
| Bsp143I | GATC | 2 cut(s) | 266, 304 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 87 |
| BspANI | GGCC | 1 cut(s) | 322 |
| BspCNI | CTCAG | 2 cut(s) | 100, 361 |
| BspHI | TCATGA | 2 cut(s) | 145, 294 |
| BspPI | GGATC | 2 cut(s) | 274, 299 |
| BsrDI | GCAATG | 3 cut(s) | 19, 61, 98 |
| BsrI | ACTGG | 1 cut(s) | 239 |
| BssMI | GATC | 2 cut(s) | 266, 304 |
| BstC8I | GCNNGC | 1 cut(s) | 379 |
| BstDEI | CTNAG | 4 cut(s) | 87, 274, 351, 369 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 312 |
| BstH2I | RGCGCY | 1 cut(s) | 182 |
| BstHHI | GCGC | 1 cut(s) | 181 |
| BstKTI | GATC | 2 cut(s) | 269, 307 |
| BstMBI | GATC | 2 cut(s) | 266, 304 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 328 |
| BstSFI | CTRYAG | 1 cut(s) | 387 |
| BstV1I | GCAGC | 1 cut(s) | 16 |
| BsuRI | GGCC | 1 cut(s) | 322 |
| BtgZI | GCGATG | 1 cut(s) | 177 |
| BtsIMutI | CAGTG | 1 cut(s) | 300 |
| Cac8I | GCNNGC | 1 cut(s) | 379 |
| CciI | TCATGA | 2 cut(s) | 145, 294 |
| CfoI | GCGC | 1 cut(s) | 181 |
| CviAII | CATG | 2 cut(s) | 146, 295 |
| CviJI | RGCY | 2 cut(s) | 278, 322 |
| CviKI_1 | RGCY | 2 cut(s) | 278, 322 |
| DdeI | CTNAG | 4 cut(s) | 87, 274, 351, 369 |
| DpnI | GATC | 2 cut(s) | 268, 306 |
| DpnII | GATC | 2 cut(s) | 266, 304 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 312 |
| EcoRI | GAATTC | 1 cut(s) | 355 |
| FaeI | CATG | 2 cut(s) | 149, 298 |
| FaiI | YATR | 6 cut(s) | 72, 114, 147, 296, 362, 389 |
| FatI | CATG | 2 cut(s) | 145, 294 |
| Fnu4HI | GCNGC | 1 cut(s) | 5 |
| Fsp4HI | GCNGC | 1 cut(s) | 5 |
| GlaI | GCGC | 1 cut(s) | 180 |
| GluI | GCNGC | 1 cut(s) | 5 |
| GsuI | CTGGAG | 1 cut(s) | 222 |
| HaeII | RGCGCY | 1 cut(s) | 182 |
| HaeIII | GGCC | 1 cut(s) | 322 |
| HhaI | GCGC | 1 cut(s) | 181 |
| Hin1II | CATG | 2 cut(s) | 149, 298 |
| Hin6I | GCGC | 1 cut(s) | 179 |
| HinP1I | GCGC | 1 cut(s) | 179 |
| HincII | GTYRAC | 1 cut(s) | 366 |
| HindII | GTYRAC | 1 cut(s) | 366 |
| HinfI | GANTC | 1 cut(s) | 151 |
| HphI | GGTGA | 1 cut(s) | 201 |
| Hpy166II | GTNNAC | 2 cut(s) | 130, 366 |
| Hpy188I | TCNGA | 2 cut(s) | 121, 344 |
| Hpy188III | TCNNGA | 3 cut(s) | 131, 146, 295 |
| Hpy8I | GTNNAC | 2 cut(s) | 130, 366 |
| HpyAV | CCTTC | 2 cut(s) | 20, 292 |
| HpyCH4V | TGCA | 5 cut(s) | 4, 17, 59, 338, 377 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 328 |
| HpyF3I | CTNAG | 4 cut(s) | 87, 274, 351, 369 |
| Hsp92II | CATG | 2 cut(s) | 149, 298 |
| HspAI | GCGC | 1 cut(s) | 179 |
| Kzo9I | GATC | 2 cut(s) | 266, 304 |
| LmnI | GCTCC | 1 cut(s) | 139 |
| LpnPI | CCDG | 3 cut(s) | 252, 300, 321 |
| Lsp1109I | GCAGC | 1 cut(s) | 16 |
| LweI | GCATC | 1 cut(s) | 151 |
| MaeIII | GTNAC | 1 cut(s) | 310 |
| MalI | GATC | 2 cut(s) | 268, 306 |
| MboI | GATC | 2 cut(s) | 266, 304 |
| MboII | GAAGA | 1 cut(s) | 35 |
| MluCI | AATT | 4 cut(s) | 100, 217, 231, 355 |
| MnlI | CCTC | 5 cut(s) | 130, 222, 246, 298, 312 |
| MroXI | GAANNNNTTC | 1 cut(s) | 126 |
| Mva1269I | GAATGC | 4 cut(s) | 81, 88, 338, 379 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 328 |
| NdeII | GATC | 2 cut(s) | 266, 304 |
| NlaIII | CATG | 2 cut(s) | 149, 298 |
| PagI | TCATGA | 2 cut(s) | 145, 294 |
| PctI | GAATGC | 4 cut(s) | 81, 88, 338, 379 |
| PdmI | GAANNNNTTC | 1 cut(s) | 126 |
| PfeI | GAWTC | 1 cut(s) | 151 |
| PkrI | GCNGC | 1 cut(s) | 6 |
| SatI | GCNGC | 1 cut(s) | 5 |
| Sau3AI | GATC | 2 cut(s) | 266, 304 |
| SetI | ASST | 4 cut(s) | 31, 214, 262, 280 |
| SfaNI | GCATC | 1 cut(s) | 151 |
| SfcI | CTRYAG | 1 cut(s) | 387 |
| Sse9I | AATT | 4 cut(s) | 100, 217, 231, 355 |
| TasI | AATT | 4 cut(s) | 100, 217, 231, 355 |
| TfiI | GAWTC | 1 cut(s) | 151 |
| TscAI | CASTG | 1 cut(s) | 307 |
| TseI | GCWGC | 1 cut(s) | 4 |
| TspDTI | ATGAA | 4 cut(s) | 36, 81, 180, 311 |
| TspRI | CASTG | 1 cut(s) | 307 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 312 |
| XapI | RAATTY | 3 cut(s) | 217, 231, 355 |
| XmnI | GAANNNNTTC | 1 cut(s) | 126 |