RLG00000020231

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000691
Physical Location & Seq
Forward (+)
62688110 .. 62691390
3281 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020231

Sequence Viewer

Length: 633 bp
ATGGCCATGCCTCAATTGCATGGCAGAAGACTTGGGCTACTGAGAGCCCCCAATGATGTGGAGGGAGATGAGAAAAAAAAAAAACTTGAAGATGAACCTGGGAGTAAAGCTGCACCTAATTGTCATGACAAGTACATTGGAAATGTGATTGAAGTCATAGTAGAGGAGGATGGTGAGATGCTACCAGCTACTGGAATAATTAAATTACCCAAGATATATTACAGTAAGATTTGTTGTGGAAAATGTCACAAGCAAGGGCATAACACGAGGACTTGTGATAGGCGCAATCAACAAGGAAGTATCTCTCAAGCTAATGCTCAGCAATGTGGCAATTCGAATGAACATAGTTCTGAAAATATTCATGATGAGGTGCATCATCATGAGATTCCATCTCAACAATCACTTGTGAAAGCACCTTTTGTTTCATCACAACCACTTCCGTCACCTCTAAATTTCTCACAAGAAATTCATCCAGTTCAAAGAAGTTCTCAACCTATTGGATCATTATCAGCTTCCTTCTCCATCATTTACACTAATCCTGTAACAGGAAGGCCAAAGAAGCAAGTTGCATTCAGAAATCCTAAGAATTCTATGTCAATTGAGAATGCTAGCAAGCCTATTTGGAGACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

211

Amino Acids

23.32

Weight (kDa)

8.88

Isoelectric Point (pI)

67.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 191
AclWI GGATC 1 cut(s) 508
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 451, 465, 586
AfaI GTAC 1 cut(s) 134
AfiI CCNNNNNNNGG 2 cut(s) 191, 545
AgsI TTSAA 3 cut(s) 89, 152, 479
AjnI CCWGG 1 cut(s) 97
AluBI AGCT 4 cut(s) 110, 188, 311, 512
AluI AGCT 4 cut(s) 110, 188, 311, 512
Alw26I GTCTC 1 cut(s) 619
AlwI GGATC 1 cut(s) 508
AlwNI CAGNNNCTG 1 cut(s) 191
AoxI GGCC 2 cut(s) 3, 551
ApeKI GCWGC 1 cut(s) 110
ApoI RAATTY 3 cut(s) 451, 465, 586
Asp700I GAANNNNTTC 1 cut(s) 357
AspLEI GCGC 1 cut(s) 285
AsuHPI GGTGA 2 cut(s) 185, 435
AsuII TTCGAA 1 cut(s) 335
AsuNHI GCTAGC 1 cut(s) 608
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 49
BauI CACGAG 1 cut(s) 265
BbsI GAAGAC 1 cut(s) 34
BbvI GCAGC 1 cut(s) 97
BccI CCATC 3 cut(s) 164, 397, 530
BciT130I CCWGG 1 cut(s) 99
BcoDI GTCTC 1 cut(s) 619
BfaI CTAG 1 cut(s) 609
BisI GCNGC 1 cut(s) 111
BlpI GCTNAGC 1 cut(s) 318
BlsI GCNGC 1 cut(s) 112
Bme1390I CCNGG 1 cut(s) 99
BmrFI CCNGG 1 cut(s) 99
BmsI GCATC 2 cut(s) 168, 382
BmtI GCTAGC 1 cut(s) 612
BpiI GAAGAC 1 cut(s) 34
Bpu1102I GCTNAGC 1 cut(s) 318
Bpu14I TTCGAA 1 cut(s) 335
BpuEI CTTGAG 1 cut(s) 291
BsaBI GATNNNNATC 1 cut(s) 505
BsaI GGTCTC 1 cut(s) 619
BsaJI CCNNGG 1 cut(s) 98
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 545
Bse1I ACTGG 2 cut(s) 196, 473
Bse3DI GCAATG 1 cut(s) 329
Bse8I GATNNNNATC 1 cut(s) 505
BseBI CCWGG 1 cut(s) 99
BseDI CCNNGG 1 cut(s) 98
BseGI GGATG 2 cut(s) 175, 469
BseJI GATNNNNATC 1 cut(s) 505
BseLI CCNNNNNNNGG 2 cut(s) 191, 545
BseMI GCAATG 1 cut(s) 329
BseMII CTCAG 2 cut(s) 32, 332
BseNI ACTGG 2 cut(s) 196, 473
BseRI GAGGAG 1 cut(s) 179
BseXI GCAGC 1 cut(s) 97
BsgI GTGCAG 1 cut(s) 96
BshFI GGCC 2 cut(s) 5, 553
BslI CCNNNNNNNGG 2 cut(s) 191, 545
BsmAI GTCTC 1 cut(s) 619
BsmI GAATGC 2 cut(s) 569, 610
BsnI GGCC 2 cut(s) 5, 553
Bso31I GGTCTC 1 cut(s) 619
Bsp119I TTCGAA 1 cut(s) 335
Bsp1286I GDGCHC 1 cut(s) 49
Bsp143I GATC 1 cut(s) 500
Bsp1720I GCTNAGC 1 cut(s) 318
BspANI GGCC 2 cut(s) 5, 553
BspCNI CTCAG 2 cut(s) 33, 331
BspHI TCATGA 3 cut(s) 124, 361, 379
BspOI GCTAGC 1 cut(s) 612
BspPI GGATC 1 cut(s) 508
BspT104I TTCGAA 1 cut(s) 335
BspTNI GGTCTC 1 cut(s) 619
BsrDI GCAATG 1 cut(s) 329
BsrI ACTGG 2 cut(s) 196, 473
BssECI CCNNGG 1 cut(s) 98
BssMI GATC 1 cut(s) 500
BssSI CACGAG 1 cut(s) 265
Bst2BI CACGAG 1 cut(s) 265
Bst2UI CCWGG 1 cut(s) 99
Bst4CI ACNGT 1 cut(s) 224
BstBI TTCGAA 1 cut(s) 335
BstC8I GCNNGC 2 cut(s) 610, 614
BstDEI CTNAG 3 cut(s) 41, 318, 582
BstENI CCTNNNNNAGG 1 cut(s) 543
BstF5I GGATG 2 cut(s) 175, 469
BstHHI GCGC 1 cut(s) 285
BstKTI GATC 1 cut(s) 503
BstMAI GTCTC 1 cut(s) 619
BstMBI GATC 1 cut(s) 500
BstMWI GCNNNNNNNGC 2 cut(s) 16, 559
BstNI CCWGG 1 cut(s) 99
BstSCI CCNGG 1 cut(s) 97
BstV1I GCAGC 1 cut(s) 97
BstV2I GAAGAC 1 cut(s) 34
BstXI CCANNNNNNTGG 1 cut(s) 58
BsuRI GGCC 2 cut(s) 5, 553
BtsCI GGATG 2 cut(s) 175, 469
Cac8I GCNNGC 2 cut(s) 610, 614
CaiI CAGNNNCTG 1 cut(s) 191
CciI TCATGA 3 cut(s) 124, 361, 379
CfoI GCGC 1 cut(s) 285
Csp6I GTAC 1 cut(s) 133
CviAII CATG 5 cut(s) 7, 20, 125, 362, 380
CviJI RGCY 9 cut(s) 5, 37, 47, 110, 188, 311, 512, 553, 616
CviKI_1 RGCY 9 cut(s) 5, 37, 47, 110, 188, 311, 512, 553, 616
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 3 cut(s) 41, 318, 582
DpnI GATC 1 cut(s) 502
DpnII GATC 1 cut(s) 500
EaeI YGGCCR 1 cut(s) 3
Eco24I GRGCYC 1 cut(s) 49
Eco31I GGTCTC 1 cut(s) 619
EcoNI CCTNNNNNAGG 1 cut(s) 543
EcoRI GAATTC 1 cut(s) 586
EcoRII CCWGG 1 cut(s) 97
EcoT38I GRGCYC 1 cut(s) 49
FaeI CATG 5 cut(s) 10, 23, 128, 365, 383
FatI CATG 5 cut(s) 6, 19, 124, 361, 379
Fnu4HI GCNGC 1 cut(s) 111
FokI GGATG 2 cut(s) 182, 456
FriOI GRGCYC 1 cut(s) 49
Fsp4HI GCNGC 1 cut(s) 111
FspBI CTAG 1 cut(s) 609
GlaI GCGC 1 cut(s) 284
GluI GCNGC 1 cut(s) 111
HaeIII GGCC 2 cut(s) 5, 553
HhaI GCGC 1 cut(s) 285
Hin1II CATG 5 cut(s) 10, 23, 128, 365, 383
Hin6I GCGC 1 cut(s) 283
HinP1I GCGC 1 cut(s) 283
HinfI GANTC 1 cut(s) 385
HphI GGTGA 2 cut(s) 185, 435
Hpy188I TCNGA 2 cut(s) 352, 575
Hpy188III TCNNGA 3 cut(s) 125, 362, 380
HpyAV CCTTC 2 cut(s) 526, 543
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4V TGCA 4 cut(s) 19, 113, 373, 569
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 559
HpyF3I CTNAG 3 cut(s) 41, 318, 582
Hsp92II CATG 5 cut(s) 10, 23, 128, 365, 383
HspAI GCGC 1 cut(s) 283
Kzo9I GATC 1 cut(s) 500
LpnPI CCDG 7 cut(s) 84, 111, 177, 198, 486, 531, 552
Lsp1109I GCAGC 1 cut(s) 97
LweI GCATC 2 cut(s) 168, 382
MaeI CTAG 1 cut(s) 609
MaeIII GTNAC 3 cut(s) 245, 441, 541
MalI GATC 1 cut(s) 502
MboI GATC 1 cut(s) 500
MboII GAAGA 2 cut(s) 39, 101
MfeI CAATTG 2 cut(s) 14, 597
MhlI GDGCHC 1 cut(s) 49
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 9 cut(s) 14, 118, 198, 203, 331, 451, 465, 586, 597
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 7 cut(s) 21, 55, 157, 160, 261, 361, 456
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 357
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 201, 631
MslI CAYNNNNRTG 1 cut(s) 378
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 99
MunI CAATTG 2 cut(s) 14, 597
Mva1269I GAATGC 2 cut(s) 569, 610
MvaI CCWGG 1 cut(s) 99
MwoI GCNNNNNNNGC 2 cut(s) 16, 559
NdeII GATC 1 cut(s) 500
NheI GCTAGC 1 cut(s) 608
NlaIII CATG 5 cut(s) 10, 23, 128, 365, 383
NmuCI GTSAC 2 cut(s) 245, 441
NspV TTCGAA 1 cut(s) 335
PagI TCATGA 3 cut(s) 124, 361, 379
PctI GAATGC 2 cut(s) 569, 610
PdmI GAANNNNTTC 1 cut(s) 357
PfeI GAWTC 1 cut(s) 385
PflMI CCANNNNNTGG 1 cut(s) 191
PkrI GCNGC 1 cut(s) 112
Psp6I CCWGG 1 cut(s) 97
PspGI CCWGG 1 cut(s) 97
PstNI CAGNNNCTG 1 cut(s) 191
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 378
SaqAI TTAA 2 cut(s) 201, 631
SatI GCNGC 1 cut(s) 111
Sau3AI GATC 1 cut(s) 500
ScrFI CCNGG 1 cut(s) 99
SduI GDGCHC 1 cut(s) 49
SfaNI GCATC 2 cut(s) 168, 382
SfuI TTCGAA 1 cut(s) 335
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 1 cut(s) 306
SmoI CTYRAG 1 cut(s) 306
Sse9I AATT 9 cut(s) 14, 118, 198, 203, 331, 451, 465, 586, 597
SspI AATATT 1 cut(s) 358
SspMI CTAG 1 cut(s) 609
StyD4I CCNGG 1 cut(s) 97
TaaI ACNGT 1 cut(s) 224
TaqI TCGA 1 cut(s) 335
TasI AATT 9 cut(s) 14, 118, 198, 203, 331, 451, 465, 586, 597
TatI WGTACW 1 cut(s) 132
TfiI GAWTC 1 cut(s) 385
Tru1I TTAA 2 cut(s) 201, 631
Tru9I TTAA 2 cut(s) 201, 631
TseFI GTSAC 2 cut(s) 245, 441
TseI GCWGC 1 cut(s) 110
Tsp45I GTSAC 2 cut(s) 245, 441
TspDTI ATGAA 5 cut(s) 108, 350, 354, 414, 458
TspGWI ACGGA 1 cut(s) 429
Van91I CCANNNNNTGG 1 cut(s) 191
XagI CCTNNNNNAGG 1 cut(s) 543
XapI RAATTY 3 cut(s) 451, 465, 586
XmnI GAANNNNTTC 1 cut(s) 357
XspI CTAG 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.