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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 2 cut(s) | 274, 296 |
| AcsI | RAATTY | 3 cut(s) | 217, 231, 352 |
| AfaI | GTAC | 1 cut(s) | 296 |
| AgsI | TTSAA | 1 cut(s) | 245 |
| AluBI | AGCT | 1 cut(s) | 278 |
| AluI | AGCT | 1 cut(s) | 278 |
| Alw26I | GTCTC | 1 cut(s) | 385 |
| AlwI | GGATC | 2 cut(s) | 274, 296 |
| AoxI | GGCC | 1 cut(s) | 317 |
| ApoI | RAATTY | 3 cut(s) | 217, 231, 352 |
| AsuHPI | GGTGA | 1 cut(s) | 201 |
| BccI | CCATC | 3 cut(s) | 44, 163, 214 |
| BcoDI | GTCTC | 1 cut(s) | 385 |
| BlpI | GCTNAGC | 1 cut(s) | 87 |
| BmsI | GCATC | 2 cut(s) | 4, 151 |
| BpmI | CTGGAG | 1 cut(s) | 222 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 87 |
| BsaI | GGTCTC | 1 cut(s) | 385 |
| Bse1I | ACTGG | 1 cut(s) | 239 |
| Bse3DI | GCAATG | 2 cut(s) | 61, 98 |
| BseMI | GCAATG | 2 cut(s) | 61, 98 |
| BseMII | CTCAG | 2 cut(s) | 101, 357 |
| BseNI | ACTGG | 1 cut(s) | 239 |
| BseRI | GAGGAG | 1 cut(s) | 152 |
| BshFI | GGCC | 1 cut(s) | 319 |
| BsmAI | GTCTC | 1 cut(s) | 385 |
| BsmI | GAATGC | 4 cut(s) | 81, 88, 335, 376 |
| BsnI | GGCC | 1 cut(s) | 319 |
| Bso31I | GGTCTC | 1 cut(s) | 385 |
| Bsp1407I | TGTACA | 1 cut(s) | 294 |
| Bsp143I | GATC | 2 cut(s) | 266, 301 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 87 |
| BspANI | GGCC | 1 cut(s) | 319 |
| BspCNI | CTCAG | 2 cut(s) | 100, 358 |
| BspHI | TCATGA | 1 cut(s) | 145 |
| BspPI | GGATC | 2 cut(s) | 274, 296 |
| BspTNI | GGTCTC | 1 cut(s) | 385 |
| BsrDI | GCAATG | 2 cut(s) | 61, 98 |
| BsrGI | TGTACA | 1 cut(s) | 294 |
| BsrI | ACTGG | 1 cut(s) | 239 |
| BssMI | GATC | 2 cut(s) | 266, 301 |
| BstAUI | TGTACA | 1 cut(s) | 294 |
| BstC8I | GCNNGC | 2 cut(s) | 376, 380 |
| BstDEI | CTNAG | 3 cut(s) | 87, 274, 366 |
| BstKTI | GATC | 2 cut(s) | 269, 304 |
| BstMAI | GTCTC | 1 cut(s) | 385 |
| BstMBI | GATC | 2 cut(s) | 266, 301 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 325 |
| BsuRI | GGCC | 1 cut(s) | 319 |
| BtgZI | GCGATG | 2 cut(s) | 27, 177 |
| BtsIMutI | CAGTG | 1 cut(s) | 297 |
| Cac8I | GCNNGC | 2 cut(s) | 376, 380 |
| CciI | TCATGA | 1 cut(s) | 145 |
| Csp6I | GTAC | 1 cut(s) | 295 |
| CviAII | CATG | 1 cut(s) | 146 |
| CviJI | RGCY | 3 cut(s) | 278, 319, 382 |
| CviKI_1 | RGCY | 3 cut(s) | 278, 319, 382 |
| CviQI | GTAC | 1 cut(s) | 295 |
| DdeI | CTNAG | 3 cut(s) | 87, 274, 366 |
| DpnI | GATC | 2 cut(s) | 268, 303 |
| DpnII | GATC | 2 cut(s) | 266, 301 |
| Eco31I | GGTCTC | 1 cut(s) | 385 |
| EcoRI | GAATTC | 1 cut(s) | 352 |
| FaeI | CATG | 1 cut(s) | 149 |
| FaiI | YATR | 5 cut(s) | 72, 114, 147, 290, 359 |
| FatI | CATG | 1 cut(s) | 145 |
| GsuI | CTGGAG | 1 cut(s) | 222 |
| HaeIII | GGCC | 1 cut(s) | 319 |
| Hin1II | CATG | 1 cut(s) | 149 |
| HincII | GTYRAC | 1 cut(s) | 363 |
| HindII | GTYRAC | 1 cut(s) | 363 |
| HinfI | GANTC | 1 cut(s) | 151 |
| HphI | GGTGA | 1 cut(s) | 201 |
| Hpy166II | GTNNAC | 2 cut(s) | 297, 363 |
| Hpy188I | TCNGA | 2 cut(s) | 341, 348 |
| Hpy188III | TCNNGA | 2 cut(s) | 131, 146 |
| Hpy8I | GTNNAC | 2 cut(s) | 297, 363 |
| HpyAV | CCTTC | 1 cut(s) | 292 |
| HpyCH4V | TGCA | 6 cut(s) | 4, 17, 59, 79, 335, 374 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 325 |
| HpyF3I | CTNAG | 3 cut(s) | 87, 274, 366 |
| Hsp92II | CATG | 1 cut(s) | 149 |
| Kzo9I | GATC | 2 cut(s) | 266, 301 |
| LmnI | GCTCC | 1 cut(s) | 139 |
| LpnPI | CCDG | 2 cut(s) | 252, 318 |
| LweI | GCATC | 2 cut(s) | 4, 151 |
| MaeIII | GTNAC | 1 cut(s) | 307 |
| MalI | GATC | 2 cut(s) | 268, 303 |
| MboI | GATC | 2 cut(s) | 266, 301 |
| MboII | GAAGA | 1 cut(s) | 361 |
| MluCI | AATT | 4 cut(s) | 100, 217, 231, 352 |
| MnlI | CCTC | 4 cut(s) | 130, 222, 246, 309 |
| MseI | TTAA | 1 cut(s) | 397 |
| Mva1269I | GAATGC | 4 cut(s) | 81, 88, 335, 376 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 325 |
| NdeII | GATC | 2 cut(s) | 266, 301 |
| NlaIII | CATG | 1 cut(s) | 149 |
| PagI | TCATGA | 1 cut(s) | 145 |
| PctI | GAATGC | 4 cut(s) | 81, 88, 335, 376 |
| PfeI | GAWTC | 1 cut(s) | 151 |
| RsaI | GTAC | 1 cut(s) | 296 |
| RsaNI | GTAC | 1 cut(s) | 295 |
| SaqAI | TTAA | 1 cut(s) | 397 |
| Sau3AI | GATC | 2 cut(s) | 266, 301 |
| SetI | ASST | 6 cut(s) | 31, 184, 214, 262, 280, 397 |
| SfaNI | GCATC | 2 cut(s) | 4, 151 |
| Sse9I | AATT | 4 cut(s) | 100, 217, 231, 352 |
| TasI | AATT | 4 cut(s) | 100, 217, 231, 352 |
| TatI | WGTACW | 1 cut(s) | 294 |
| TfiI | GAWTC | 1 cut(s) | 151 |
| Tru1I | TTAA | 1 cut(s) | 397 |
| Tru9I | TTAA | 1 cut(s) | 397 |
| TscAI | CASTG | 1 cut(s) | 304 |
| TspDTI | ATGAA | 3 cut(s) | 36, 81, 180 |
| TspRI | CASTG | 1 cut(s) | 304 |
| XapI | RAATTY | 3 cut(s) | 217, 231, 352 |