Rorug02G0070900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000691
Physical Location & Seq
Reverse (-)
5507963 .. 5513298
5336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0070900.1

Sequence Viewer

Length: 1437 bp
ATGGAATCAAGAGGGATTAAGATCTCAAAGGGCGTGTTGCAGAGTGATGAATTATCTCAATACATCTTGGAGACTAGTGTGTATCCACGTGAACCAGCGCCTCTCAAGGAGCTAAGGGATGCCACTGCCAGCCACCCTCGTGCTGGATTGGCTACTGCACCAGATGCAGCTCAGTTACTAGCCATGCTGTTGAGGCTCATAGATGCAAAGAAGACAATTGAAATTGGTGTTTTCACGGGATATTCTCTTCTGCTCACTGCGCTTACAATTCCTGAGGATGGCAAGATTGTAGCCATAGATATAAATCGTGAGACATATGATCAAATAGGCTTGCCGATCATAAAGAAAGCTGGTGTTGAGCACAAAATTGACTTCATTGAATCTGAGGCTGTACCAGTTCTTGATAAGCTATTCGAACATCCTGAAAATGAAGGCAGTTTCGACTTTGCCTATGTGGATGCCGACAAGGTTAACAATTGGAATTACCACGAGAGACTACTGAAGCTGTTGAAGGTGGGTGGCTTAGTTGTCTATGACAACACACTCTGCAGAGGATCAGTCGCGTTGCCTGATGAATTAGTTCCAGAGCCGAGGAAACAGCGCAGACAGCTAGCCATTGAGCATAACAAGACACTTGCAGCCGATCCACGTAATTTCACAAGTACCACATTACTGATTACGTTTCTAGCCAACAATGAATGTTTGGATCAGCAATATATTCTGAGAGAAATGGAGCAGAACAAGAAAAAGGTATCGAAGGGACTATTGCAGAGTGATGACTTGTATCAGTATATCTTGGAGACTAGCGTGTATCCGCGTGAACCGGAGCCTCTCAAGGAGCTGAGAGAGGCCACTGCCAGCCACCCCGGGGCTGAGATGGCTACTGCACCAGATGCAGGCCAGTTGATGACCATGCTGTTGGGGCTAGTAGATGCAAAGAAGACGATTGAAGTTGGAGTTTTTACCGGATACTCTCTTCTCCTCACTGCTCTTACAATTCCTGAGGATGGCAAGATTGTAGCCATAGATGTAGATCGTGAGCCATATGATCAAATAGGATTTCCAATCATAAAGAAAGCTGGTGTTGAGCACAAAATTGACCTCATTGAATCTGAGGCCCTACCCGTTCTTGATAAGCTATTGGAACAACATGAAAATGAAGGAAGTTTCGACTTTGCTTTCGTTGATGCAGACAAGGTTAACTATTGCAATTACCATGAGAGACTAATGAAGCTGCTGAAGGTGGGGGGTATGGCTGTGTATGATAACACTCTTTGGGGAGGGACAGTTGTAATGCCTGTTGAGTCGACTCCGGAATGGATGAAACCAAGTAGACAGTCTATTATGGAGCTCAATAAGTTGCTCGCTGCTGATACTCGTGTCCAAATTTCGCATGCTGCTTTGGGTGATGGGATCACAGTTTGTAGGCGACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

478

Amino Acids

53.31

Weight (kDa)

5.09

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_3 PF01596 24 - 217 2.7e-63 O-methyltransferase
Methyltransf_24 PF13578 73 - 180 3.5e-08 Methyltransferase domain
Methyltransf_3 PF01596 267 - 478 2e-75 O-methyltransferase
Methyltransf_24 PF13578 316 - 423 2.3e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 1307, 1333
AccII CGCG 2 cut(s) 563, 817
AccIII TCCGGA 1 cut(s) 1312
AciI CCGC 1 cut(s) 815
AclWI GGATC 4 cut(s) 562, 638, 714, 1421
AcsI RAATTY 1 cut(s) 1386
AcuI CTGAAG 2 cut(s) 521, 1259
AcvI CACGTG 1 cut(s) 89
AfaI GTAC 2 cut(s) 393, 664
AfiI CCNNNNNNNGG 5 cut(s) 143, 278, 868, 896, 1007
AgsI TTSAA 5 cut(s) 221, 380, 511, 950, 1109
AhlI ACTAGT 1 cut(s) 74
AleI CACNNNNGTG 1 cut(s) 138
Alw21I GWGCWC 3 cut(s) 363, 1092, 1353
Alw26I GTCTC 5 cut(s) 65, 305, 487, 794, 1216
AlwI GGATC 4 cut(s) 562, 638, 714, 1421
Ama87I CYCGRG 1 cut(s) 866
Aor13HI TCCGGA 1 cut(s) 1312
AoxI GGCC 3 cut(s) 849, 898, 1116
ApeKI GCWGC 5 cut(s) 167, 638, 1234, 1367, 1397
ApoI RAATTY 1 cut(s) 1386
ArsI GACNNNNNNTTYG 2 cut(s) 1163, 1195
Asp700I GAANNNNTTC 1 cut(s) 579
AspLEI GCGC 3 cut(s) 100, 262, 603
AspS9I GGNCC 1 cut(s) 1117
AsuC2I CCSGG 2 cut(s) 867, 868
AsuHPI GGTGA 1 cut(s) 1418
AsuII TTCGAA 1 cut(s) 414
AsuNHI GCTAGC 1 cut(s) 610
AvaI CYCGRG 1 cut(s) 866
AxyI CCTNAGG 2 cut(s) 273, 1002
BaeI ACNNNNGTAYC 2 cut(s) 1365, 1398
BanII GRGCYC 1 cut(s) 1353
BauI CACGAG 3 cut(s) 138, 488, 1377
BbrPI CACGTG 1 cut(s) 89
BbsI GAAGAC 2 cut(s) 218, 947
Bbv12I GWGCWC 3 cut(s) 363, 1092, 1353
BbvI GCAGC 5 cut(s) 179, 650, 1221, 1354, 1384
BccI CCATC 4 cut(s) 272, 871, 1001, 1403
BciVI GTATCC 3 cut(s) 93, 822, 962
BclI TGATCA 2 cut(s) 319, 1048
BcnI CCSGG 2 cut(s) 867, 868
BcoDI GTCTC 5 cut(s) 65, 305, 487, 794, 1216
BcuI ACTAGT 1 cut(s) 74
BfaI CTAG 6 cut(s) 75, 179, 611, 686, 804, 926
BfmI CTRYAG 1 cut(s) 547
BfoI RGCGCY 1 cut(s) 101
BfuI GTATCC 3 cut(s) 93, 822, 962
BglII AGATCT 1 cut(s) 21
BisI GCNGC 5 cut(s) 168, 639, 1235, 1368, 1398
BlsI GCNGC 5 cut(s) 169, 640, 1236, 1369, 1399
Bme1390I CCNGG 2 cut(s) 867, 868
BmeT110I CYCGRG 1 cut(s) 866
BmgT120I GGNCC 1 cut(s) 1117
BmiI GGNNCC 1 cut(s) 828
BmrFI CCNGG 2 cut(s) 867, 868
BmsI GCATC 7 cut(s) 109, 154, 193, 448, 883, 922, 1177
BmtI GCTAGC 1 cut(s) 614
BpiI GAAGAC 2 cut(s) 218, 947
Bpu10I CCTNAGC 1 cut(s) 113
Bpu14I TTCGAA 1 cut(s) 414
BpuEI CTTGAG 2 cut(s) 89, 818
BpuMI CCSGG 2 cut(s) 867, 868
BsaAI YACGTR 2 cut(s) 89, 650
BsaBI GATNNNNATC 3 cut(s) 20, 303, 1032
BsaJI CCNNGG 4 cut(s) 590, 865, 866, 867
BsaWI WCCGGW 3 cut(s) 823, 965, 1312
BsaXI ACNNNNNCTCC 2 cut(s) 1272, 1302
Bsc4I CCNNNNNNNGG 5 cut(s) 143, 278, 868, 896, 1007
Bse1I ACTGG 2 cut(s) 395, 901
Bse21I CCTNAGG 2 cut(s) 273, 1002
Bse8I GATNNNNATC 3 cut(s) 20, 303, 1032
BseAI TCCGGA 1 cut(s) 1312
BseDI CCNNGG 4 cut(s) 590, 865, 866, 867
BseGI GGATG 6 cut(s) 124, 283, 418, 463, 1012, 1326
BseJI GATNNNNATC 3 cut(s) 20, 303, 1032
BseLI CCNNNNNNNGG 5 cut(s) 143, 278, 868, 896, 1007
BseMII CTCAG 8 cut(s) 185, 264, 375, 713, 833, 864, 993, 1104
BseNI ACTGG 2 cut(s) 395, 901
BseRI GAGGAG 1 cut(s) 971
BseXI GCAGC 5 cut(s) 179, 650, 1221, 1354, 1384
BsgI GTGCAG 2 cut(s) 141, 870
Bsh1236I CGCG 2 cut(s) 563, 817
BshFI GGCC 3 cut(s) 851, 900, 1118
BsiHKAI GWGCWC 3 cut(s) 363, 1092, 1353
BsiHKCI CYCGRG 1 cut(s) 866
BsiSI CCGG 4 cut(s) 824, 867, 966, 1313
BslFI GGGAC 2 cut(s) 774, 1297
BslI CCNNNNNNNGG 5 cut(s) 143, 278, 868, 896, 1007
BsmAI GTCTC 5 cut(s) 65, 305, 487, 794, 1216
BsmFI GGGAC 2 cut(s) 774, 1297
BsnI GGCC 3 cut(s) 851, 900, 1118
BsoBI CYCGRG 1 cut(s) 866
Bsp119I TTCGAA 1 cut(s) 414
Bsp1286I GDGCHC 3 cut(s) 363, 1092, 1353
Bsp13I TCCGGA 1 cut(s) 1312
Bsp143I GATC 9 cut(s) 21, 319, 336, 554, 643, 706, 1033, 1048, 1413
BspACI CCGC 1 cut(s) 815
BspANI GGCC 3 cut(s) 851, 900, 1118
BspCNI CTCAG 8 cut(s) 184, 265, 376, 714, 834, 865, 994, 1105
BspEI TCCGGA 1 cut(s) 1312
BspFNI CGCG 2 cut(s) 563, 817
BspLI GGNNCC 1 cut(s) 828
BspMAI CTGCAG 1 cut(s) 551
BspOI GCTAGC 1 cut(s) 614
BspPI GGATC 4 cut(s) 562, 638, 714, 1421
BspT104I TTCGAA 1 cut(s) 414
BsrI ACTGG 2 cut(s) 395, 901
BssECI CCNNGG 4 cut(s) 590, 865, 866, 867
BssMI GATC 9 cut(s) 21, 319, 336, 554, 643, 706, 1033, 1048, 1413
BssSI CACGAG 3 cut(s) 138, 488, 1377
Bst2BI CACGAG 3 cut(s) 138, 488, 1377
Bst4CI ACNGT 3 cut(s) 1288, 1338, 1420
Bst6I CTCTTC 2 cut(s) 252, 981
BstAPI GCANNNNNTGC 2 cut(s) 164, 893
BstBAI YACGTR 2 cut(s) 89, 650
BstBI TTCGAA 1 cut(s) 414
BstC8I GCNNGC 7 cut(s) 130, 332, 612, 859, 898, 1365, 1395
BstF5I GGATG 6 cut(s) 124, 283, 418, 463, 1012, 1326
BstFNI CGCG 2 cut(s) 563, 817
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 3 cut(s) 100, 262, 603
BstKTI GATC 9 cut(s) 24, 322, 339, 557, 646, 709, 1036, 1051, 1416
BstMAI GTCTC 5 cut(s) 65, 305, 487, 794, 1216
BstMBI GATC 9 cut(s) 21, 319, 336, 554, 643, 706, 1033, 1048, 1413
BstMWI GCNNNNNNNGC 8 cut(s) 149, 164, 193, 259, 607, 878, 893, 922
BstNSI RCATGY 1 cut(s) 1397
BstSCI CCNGG 2 cut(s) 865, 866
BstSFI CTRYAG 1 cut(s) 547
BstUI CGCG 2 cut(s) 563, 817
BstV1I GCAGC 5 cut(s) 179, 650, 1221, 1354, 1384
BstV2I GAAGAC 2 cut(s) 218, 947
BstX2I RGATCY 1 cut(s) 21
BstXI CCANNNNNNTGG 1 cut(s) 919
BstYI RGATCY 1 cut(s) 21
Bsu36I CCTNAGG 2 cut(s) 273, 1002
BsuI GTATCC 3 cut(s) 93, 822, 962
BsuRI GGCC 3 cut(s) 851, 900, 1118
BtsCI GGATG 6 cut(s) 124, 283, 418, 463, 1012, 1326
BtsI GCAGTG 4 cut(s) 123, 255, 852, 984
BtsIMutI CAGTG 4 cut(s) 123, 255, 852, 984
Cac8I GCNNGC 7 cut(s) 130, 332, 612, 859, 898, 1365, 1395
CfoI GCGC 3 cut(s) 100, 262, 603
Cfr13I GGNCC 1 cut(s) 1117
Cfr9I CCCGGG 1 cut(s) 866
Csp6I GTAC 2 cut(s) 392, 663
CviAII CATG 5 cut(s) 184, 913, 1151, 1217, 1394
CviQI GTAC 2 cut(s) 392, 663
DpnI GATC 9 cut(s) 23, 321, 338, 556, 645, 708, 1035, 1050, 1415
DpnII GATC 9 cut(s) 21, 319, 336, 554, 643, 706, 1033, 1048, 1413
Eam1104I CTCTTC 2 cut(s) 252, 981
EarI CTCTTC 2 cut(s) 252, 981
Ecl136II GAGCTC 1 cut(s) 1351
Eco24I GRGCYC 1 cut(s) 1353
Eco53kI GAGCTC 1 cut(s) 1351
Eco57I CTGAAG 2 cut(s) 521, 1259
Eco72I CACGTG 1 cut(s) 89
Eco81I CCTNAGG 2 cut(s) 273, 1002
Eco88I CYCGRG 1 cut(s) 866
EcoICRI GAGCTC 1 cut(s) 1351
EcoO109I RGGNCCY 1 cut(s) 1117
EcoT38I GRGCYC 1 cut(s) 1353
FaeI CATG 5 cut(s) 187, 916, 1154, 1220, 1397
FaqI GGGAC 2 cut(s) 774, 1297
FatI CATG 5 cut(s) 183, 912, 1150, 1216, 1393
FauNDI CATATG 2 cut(s) 316, 1045
FbaI TGATCA 2 cut(s) 319, 1048
FblI GTMKAC 2 cut(s) 1307, 1333
Fnu4HI GCNGC 5 cut(s) 168, 639, 1235, 1368, 1398
FokI GGATG 6 cut(s) 131, 290, 405, 470, 1019, 1333
FriOI GRGCYC 1 cut(s) 1353
Fsp4HI GCNGC 5 cut(s) 168, 639, 1235, 1368, 1398
FspBI CTAG 6 cut(s) 75, 179, 611, 686, 804, 926
GlaI GCGC 3 cut(s) 99, 261, 602
GluI GCNGC 5 cut(s) 168, 639, 1235, 1368, 1398
HaeII RGCGCY 1 cut(s) 101
HaeIII GGCC 3 cut(s) 851, 900, 1118
HapII CCGG 4 cut(s) 824, 867, 966, 1313
HhaI GCGC 3 cut(s) 100, 262, 603
Hin1II CATG 5 cut(s) 187, 916, 1154, 1220, 1397
Hin6I GCGC 3 cut(s) 98, 260, 601
HinP1I GCGC 3 cut(s) 98, 260, 601
HincII GTYRAC 3 cut(s) 472, 1201, 1308
HindII GTYRAC 3 cut(s) 472, 1201, 1308
HinfI GANTC 5 cut(s) 5, 380, 1109, 1304, 1309
HpaI GTTAAC 2 cut(s) 472, 1201
HpaII CCGG 4 cut(s) 824, 867, 966, 1313
HphI GGTGA 1 cut(s) 1418
Hpy166II GTNNAC 6 cut(s) 92, 472, 821, 1201, 1308, 1334
Hpy188I TCNGA 3 cut(s) 385, 723, 1114
Hpy8I GTNNAC 6 cut(s) 92, 472, 821, 1201, 1308, 1334
HpyAV CCTTC 5 cut(s) 425, 505, 751, 1154, 1234
HpyCH4III ACNGT 3 cut(s) 1288, 1338, 1420
HpyCH4IV ACGT 3 cut(s) 88, 649, 680
HpyF10VI GCNNNNNNNGC 8 cut(s) 149, 164, 193, 259, 607, 878, 893, 922
HpySE526I ACGT 3 cut(s) 88, 649, 680
Hsp92II CATG 5 cut(s) 187, 916, 1154, 1220, 1397
HspAI GCGC 3 cut(s) 98, 260, 601
Kpn2I TCCGGA 1 cut(s) 1312
Ksp22I TGATCA 2 cut(s) 319, 1048
KspAI GTTAAC 2 cut(s) 472, 1201
Kzo9I GATC 9 cut(s) 21, 319, 336, 554, 643, 706, 1033, 1048, 1413
LmnI GCTCC 5 cut(s) 109, 733, 826, 838, 1348
Lsp1109I GCAGC 5 cut(s) 179, 650, 1221, 1354, 1384
LweI GCATC 7 cut(s) 109, 154, 193, 448, 883, 922, 1177
MaeI CTAG 6 cut(s) 75, 179, 611, 686, 804, 926
MaeII ACGT 3 cut(s) 88, 649, 680
MaeIII GTNAC 1 cut(s) 174
MalI GATC 9 cut(s) 23, 321, 338, 556, 645, 708, 1035, 1050, 1415
MboI GATC 9 cut(s) 21, 319, 336, 554, 643, 706, 1033, 1048, 1413
MboII GAAGA 4 cut(s) 223, 239, 952, 968
MfeI CAATTG 2 cut(s) 216, 475
MflI RGATCY 1 cut(s) 21
MhlI GDGCHC 3 cut(s) 363, 1092, 1353
MlyI GAGTC 2 cut(s) 1303, 1313
MmeI TCCRAC 1 cut(s) 934
MroI TCCGGA 1 cut(s) 1312
MroXI GAANNNNTTC 1 cut(s) 579
MseI TTAA 3 cut(s) 18, 471, 1200
MslI CAYNNNNRTG 2 cut(s) 138, 1155
MspI CCGG 4 cut(s) 824, 867, 966, 1313
MspR9I CCNGG 2 cut(s) 867, 868
MunI CAATTG 2 cut(s) 216, 475
MvnI CGCG 2 cut(s) 563, 817
MwoI GCNNNNNNNGC 8 cut(s) 149, 164, 193, 259, 607, 878, 893, 922
NciI CCSGG 2 cut(s) 867, 868
NdeI CATATG 2 cut(s) 316, 1045
NdeII GATC 9 cut(s) 21, 319, 336, 554, 643, 706, 1033, 1048, 1413
NheI GCTAGC 1 cut(s) 610
NlaIII CATG 5 cut(s) 187, 916, 1154, 1220, 1397
NlaIV GGNNCC 1 cut(s) 828
NmeAIII GCCGAG 1 cut(s) 615
NspI RCATGY 1 cut(s) 1397
NspV TTCGAA 1 cut(s) 414
OliI CACNNNNGTG 1 cut(s) 138
PaeI GCATGC 1 cut(s) 1397
PdmI GAANNNNTTC 1 cut(s) 579
PfeI GAWTC 3 cut(s) 5, 380, 1109
PkrI GCNGC 5 cut(s) 169, 640, 1236, 1369, 1399
PleI GAGTC 2 cut(s) 1303, 1312
PmaCI CACGTG 1 cut(s) 89
PmlI CACGTG 1 cut(s) 89
PpsI GAGTC 2 cut(s) 1303, 1312
Ppu21I YACGTR 2 cut(s) 89, 650
Psp124BI GAGCTC 1 cut(s) 1353
PspCI CACGTG 1 cut(s) 89
PspN4I GGNNCC 1 cut(s) 828
PspPI GGNCC 1 cut(s) 1117
PstI CTGCAG 1 cut(s) 551
PsuI RGATCY 1 cut(s) 21
RsaI GTAC 2 cut(s) 393, 664
RsaNI GTAC 2 cut(s) 392, 663
RseI CAYNNNNRTG 2 cut(s) 138, 1155
SacI GAGCTC 1 cut(s) 1353
SalI GTCGAC 1 cut(s) 1306
SaqAI TTAA 3 cut(s) 18, 471, 1200
SatI GCNGC 5 cut(s) 168, 639, 1235, 1368, 1398
Sau3AI GATC 9 cut(s) 21, 319, 336, 554, 643, 706, 1033, 1048, 1413
Sau96I GGNCC 1 cut(s) 1117
SchI GAGTC 2 cut(s) 1303, 1313
ScrFI CCNGG 2 cut(s) 867, 868
SduI GDGCHC 3 cut(s) 363, 1092, 1353
SfaNI GCATC 7 cut(s) 109, 154, 193, 448, 883, 922, 1177
SfcI CTRYAG 1 cut(s) 547
SfuI TTCGAA 1 cut(s) 414
SmaI CCCGGG 1 cut(s) 868
SmiMI CAYNNNNRTG 2 cut(s) 138, 1155
SmlI CTYRAG 2 cut(s) 104, 833
SmoI CTYRAG 2 cut(s) 104, 833
SpeI ACTAGT 1 cut(s) 74
SphI GCATGC 1 cut(s) 1397
SsiI CCGC 1 cut(s) 815
SspMI CTAG 6 cut(s) 75, 179, 611, 686, 804, 926
SstI GAGCTC 1 cut(s) 1353
StyD4I CCNGG 2 cut(s) 865, 866
TaaI ACNGT 3 cut(s) 1288, 1338, 1420
TaiI ACGT 3 cut(s) 91, 652, 683
TaqI TCGA 5 cut(s) 414, 441, 755, 1170, 1307
TfiI GAWTC 3 cut(s) 5, 380, 1109
Tru1I TTAA 3 cut(s) 18, 471, 1200
Tru9I TTAA 3 cut(s) 18, 471, 1200
TscAI CASTG 4 cut(s) 130, 262, 859, 991
TseI GCWGC 5 cut(s) 167, 638, 1234, 1367, 1397
TspDTI ATGAA 9 cut(s) 63, 364, 444, 588, 711, 1167, 1173, 1244, 1337
TspMI CCCGGG 1 cut(s) 866
TspRI CASTG 4 cut(s) 130, 262, 859, 991
XapI RAATTY 1 cut(s) 1386
XceI RCATGY 1 cut(s) 1397
XcmI CCANNNNNNNNNTGG 1 cut(s) 140
XmaI CCCGGG 1 cut(s) 866
XmiI GTMKAC 2 cut(s) 1307, 1333
XmnI GAANNNNTTC 1 cut(s) 579
XspI CTAG 6 cut(s) 75, 179, 611, 686, 804, 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.