FvH4_4g09131

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000691
Physical Location & Seq
Reverse (-)
10482205 .. 10483547
1343 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g09131.t1

Sequence Viewer

Length: 1071 bp
ATGGTCAGGTTGGCCAATTGGAGAAACTCCGGTGCAAGGTGGAGGTGCAAGGTGGGACCAAGAATTGAGAAACTGTTGAAGAAAAATGCTGCATGGGCCAATGAGTACAGGGCTCTTGAATCTAGTTCAAATAGATTTGAGCTACAAGGTAGAGGGGTTTGCTGTGAATCTGGAGTAATTGCACAACACTCTGTGCAATTAGACACTATGTCATGCACTTGTAGGAGATGGAATCTATGTGAACTTCCATGTGGGCATGCAATTGCTGCAATCTACTCTAAAGGTTGGCTGCCAGATGATTACGTCTCTGAATGGTATACACTGAAAAGGTATATGCAGTCTTATGAAGTGATGCTCAATCCAATATCTGGTGTTGATGAGTGGGTACACATTGAGAGAAAGATAGCATCTCCACTGTATAAGAGGCAACCAGGGAGGCCCAAGACAAAAAGAACCTTAGAACCAGGTGAAGCACCACCACCCACTGGTATTGAGAAGTTGCCAGGGACTTATTATGCTCAAGTTAATTGTGGAGAATGTGGCAAGAAAGGCCACAACAAAAGAATTTGTGCAAGAAGGAACCAGGTTTCCCATGCCAATGTTGTTGTTGAAGAAGAGAATGTTCAACTACTTATACCTGAAGAAAACCAAGCTCATCCTCAGAGTCCAAATGGAGCACCACATCCTACCCAAGAATCAAGTCTTAATGATGTTAACCATGTGGTCTCTCAAGCATCATCTATGCCATGCTTTAACCATCATGACACTGTGAATGTGAATGGGAATGGGAATGACAATGACAATGTCAACTTGAATGTTAATGACTTTGTGAATGAAGACTCTACAACTGATATTGACATCTCTATGTCATCTCAACACACTAACGTGGTTCAGCCTACTTCTCAGGGACCATCTGTTGCTACAACTTCAAAGGCAAAGATAAATGAGCCAAGAAAATCAACCAGACTTGCTGCTTCTAAAGCCAAAGGTGATGTTCAAGTTCCAAAGAACCAACACTACCAGGACAAGTACTTTAGCCGACGAAATATTTTCGTCGGCCTGTTATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

357

Amino Acids

39.88

Weight (kDa)

8.79

Isoelectric Point (pI)

53.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIM PF04434 65 - 94 1.3e-06 SWIM zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 368, 485
AccI GTMKAC 1 cut(s) 317
AcoI YGGCCR 1 cut(s) 12
AcsI RAATTY 1 cut(s) 564
AcuI CTGAAG 1 cut(s) 660
AdeI CACNNNGTG 1 cut(s) 193
AfaI GTAC 3 cut(s) 107, 387, 1031
AfiI CCNNNNNNNGG 3 cut(s) 36, 368, 485
AgsI TTSAA 8 cut(s) 79, 119, 129, 611, 626, 814, 930, 998
AhdI GACNNNNNGTC 1 cut(s) 208
AjnI CCWGG 5 cut(s) 430, 463, 502, 582, 1020
AleI CACNNNNGTG 1 cut(s) 884
AloI GAACNNNNNNTCC 2 cut(s) 572, 604
AluBI AGCT 2 cut(s) 142, 653
AluI AGCT 2 cut(s) 142, 653
Alw21I GWGCWC 1 cut(s) 679
Alw26I GTCTC 2 cut(s) 310, 730
AoxI GGCC 5 cut(s) 12, 96, 437, 550, 1057
ApeKI GCWGC 4 cut(s) 89, 266, 289, 971
ApoI RAATTY 1 cut(s) 564
AspS9I GGNCC 4 cut(s) 56, 96, 438, 908
AsuHPI GGTGA 2 cut(s) 479, 1001
AvaII GGWCC 2 cut(s) 56, 908
BalI TGGCCA 1 cut(s) 14
BanII GRGCYC 1 cut(s) 115
BbsI GAAGAC 1 cut(s) 843
Bbv12I GWGCWC 1 cut(s) 679
BbvI GCAGC 4 cut(s) 76, 253, 276, 958
BccI CCATC 3 cut(s) 222, 765, 919
BciT130I CCWGG 5 cut(s) 432, 465, 504, 584, 1022
BcoDI GTCTC 2 cut(s) 310, 730
BfaI CTAG 1 cut(s) 123
BisI GCNGC 4 cut(s) 90, 267, 290, 972
BlsI GCNGC 4 cut(s) 91, 268, 291, 973
BmcAI AGTACT 1 cut(s) 1031
Bme1390I CCNGG 5 cut(s) 432, 465, 504, 584, 1022
Bme18I GGWCC 2 cut(s) 56, 908
BmeRI GACNNNNNGTC 1 cut(s) 208
BmgT120I GGNCC 4 cut(s) 56, 96, 438, 908
BmiI GGNNCC 3 cut(s) 57, 581, 909
BmrFI CCNGG 5 cut(s) 432, 465, 504, 584, 1022
BmsI GCATC 3 cut(s) 342, 416, 743
BpiI GAAGAC 1 cut(s) 843
BpmI CTGGAG 1 cut(s) 192
BpuEI CTTGAG 2 cut(s) 504, 714
BsaI GGTCTC 1 cut(s) 730
BsaJI CCNNGG 2 cut(s) 431, 503
BsaWI WCCGGW 1 cut(s) 29
Bsc4I CCNNNNNNNGG 3 cut(s) 36, 368, 485
Bse1I ACTGG 1 cut(s) 490
BseBI CCWGG 5 cut(s) 432, 465, 504, 584, 1022
BseDI CCNNGG 2 cut(s) 431, 503
BseGI GGATG 2 cut(s) 655, 682
BseLI CCNNNNNNNGG 3 cut(s) 36, 368, 485
BseMII CTCAG 2 cut(s) 674, 917
BseNI ACTGG 1 cut(s) 490
BseXI GCAGC 4 cut(s) 76, 253, 276, 958
BshFI GGCC 5 cut(s) 14, 98, 439, 552, 1059
BsiHKAI GWGCWC 1 cut(s) 679
BsiSI CCGG 1 cut(s) 30
BslFI GGGAC 3 cut(s) 69, 520, 921
BslI CCNNNNNNNGG 3 cut(s) 36, 368, 485
BsmAI GTCTC 2 cut(s) 310, 730
BsmBI CGTCTC 1 cut(s) 310
BsmFI GGGAC 3 cut(s) 69, 520, 921
BsnI GGCC 5 cut(s) 14, 98, 439, 552, 1059
Bso31I GGTCTC 1 cut(s) 730
Bsp1286I GDGCHC 2 cut(s) 115, 679
BspANI GGCC 5 cut(s) 14, 98, 439, 552, 1059
BspCNI CTCAG 2 cut(s) 673, 916
BspHI TCATGA 1 cut(s) 760
BspLI GGNNCC 3 cut(s) 57, 581, 909
BspTNI GGTCTC 1 cut(s) 730
BsrI ACTGG 1 cut(s) 490
BssECI CCNNGG 2 cut(s) 431, 503
BssNAI GTATAC 1 cut(s) 318
Bst1107I GTATAC 1 cut(s) 318
Bst2UI CCWGG 5 cut(s) 432, 465, 504, 584, 1022
Bst4CI ACNGT 3 cut(s) 75, 417, 769
Bst6I CTCTTC 1 cut(s) 609
BstAPI GCANNNNNTGC 1 cut(s) 266
BstC8I GCNNGC 1 cut(s) 258
BstDEI CTNAG 3 cut(s) 457, 660, 903
BstF5I GGATG 2 cut(s) 655, 682
BstMAI GTCTC 2 cut(s) 310, 730
BstMWI GCNNNNNNNGC 4 cut(s) 95, 266, 549, 980
BstNI CCWGG 5 cut(s) 432, 465, 504, 584, 1022
BstNSI RCATGY 1 cut(s) 260
BstSCI CCNGG 5 cut(s) 430, 463, 502, 582, 1020
BstV1I GCAGC 4 cut(s) 76, 253, 276, 958
BstV2I GAAGAC 1 cut(s) 843
BstZ17I GTATAC 1 cut(s) 318
BsuRI GGCC 5 cut(s) 14, 98, 439, 552, 1059
BtsCI GGATG 2 cut(s) 655, 682
BtsIMutI CAGTG 4 cut(s) 320, 413, 483, 765
Cac8I GCNNGC 1 cut(s) 258
CciI TCATGA 1 cut(s) 760
Cfr13I GGNCC 4 cut(s) 56, 96, 438, 908
CsiI ACCWGGT 2 cut(s) 463, 582
Csp6I GTAC 3 cut(s) 106, 386, 1030
CspCI CAANNNNNGTGG 2 cut(s) 472, 507
CviAII CATG 8 cut(s) 93, 213, 249, 257, 593, 719, 747, 761
CviQI GTAC 3 cut(s) 106, 386, 1030
DdeI CTNAG 3 cut(s) 457, 660, 903
DraIII CACNNNGTG 1 cut(s) 193
DriI GACNNNNNGTC 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 12
Eam1104I CTCTTC 1 cut(s) 609
Eam1105I GACNNNNNGTC 1 cut(s) 208
EarI CTCTTC 1 cut(s) 609
Eco24I GRGCYC 1 cut(s) 115
Eco31I GGTCTC 1 cut(s) 730
Eco47I GGWCC 2 cut(s) 56, 908
Eco57I CTGAAG 1 cut(s) 660
EcoRII CCWGG 5 cut(s) 430, 463, 502, 582, 1020
EcoT38I GRGCYC 1 cut(s) 115
Esp3I CGTCTC 1 cut(s) 310
FaeI CATG 8 cut(s) 96, 216, 252, 260, 596, 722, 750, 764
FaqI GGGAC 3 cut(s) 69, 520, 921
FatI CATG 8 cut(s) 92, 212, 248, 256, 592, 718, 746, 760
FblI GTMKAC 1 cut(s) 317
Fnu4HI GCNGC 4 cut(s) 90, 267, 290, 972
FokI GGATG 2 cut(s) 642, 669
FriOI GRGCYC 1 cut(s) 115
Fsp4HI GCNGC 4 cut(s) 90, 267, 290, 972
FspBI CTAG 1 cut(s) 123
GluI GCNGC 4 cut(s) 90, 267, 290, 972
GsuI CTGGAG 1 cut(s) 192
HaeIII GGCC 5 cut(s) 14, 98, 439, 552, 1059
HapII CCGG 1 cut(s) 30
Hin1II CATG 8 cut(s) 96, 216, 252, 260, 596, 722, 750, 764
HincII GTYRAC 2 cut(s) 715, 808
HindII GTYRAC 2 cut(s) 715, 808
HinfI GANTC 6 cut(s) 119, 167, 232, 664, 695, 839
HpaI GTTAAC 1 cut(s) 715
HpaII CCGG 1 cut(s) 30
HphI GGTGA 2 cut(s) 479, 1001
Hpy166II GTNNAC 5 cut(s) 242, 318, 388, 715, 808
Hpy188I TCNGA 2 cut(s) 310, 663
Hpy188III TCNNGA 3 cut(s) 116, 171, 761
Hpy8I GTNNAC 5 cut(s) 242, 318, 388, 715, 808
Hpy99I CGWCG 2 cut(s) 1044, 1058
HpyAV CCTTC 1 cut(s) 570
HpyCH4III ACNGT 3 cut(s) 75, 417, 769
HpyCH4IV ACGT 2 cut(s) 303, 885
HpyF10VI GCNNNNNNNGC 4 cut(s) 95, 266, 549, 980
HpyF3I CTNAG 3 cut(s) 457, 660, 903
HpySE526I ACGT 2 cut(s) 303, 885
Hsp92II CATG 8 cut(s) 96, 216, 252, 260, 596, 722, 750, 764
KspAI GTTAAC 1 cut(s) 715
LmnI GCTCC 1 cut(s) 674
Lsp1109I GCAGC 4 cut(s) 76, 253, 276, 958
LweI GCATC 3 cut(s) 342, 416, 743
MabI ACCWGGT 2 cut(s) 463, 582
MaeI CTAG 1 cut(s) 123
MaeII ACGT 2 cut(s) 303, 885
MboII GAAGA 5 cut(s) 91, 623, 626, 653, 848
MfeI CAATTG 2 cut(s) 16, 261
MhlI GDGCHC 2 cut(s) 115, 679
MlsI TGGCCA 1 cut(s) 14
MluCI AATT 7 cut(s) 16, 63, 177, 197, 261, 526, 564
MluNI TGGCCA 1 cut(s) 14
MlyI GAGTC 2 cut(s) 673, 833
MnlI CCTC 5 cut(s) 36, 146, 417, 429, 669
Mox20I TGGCCA 1 cut(s) 14
MscI TGGCCA 1 cut(s) 14
MseI TTAA 5 cut(s) 525, 705, 714, 753, 819
MslI CAYNNNNRTG 3 cut(s) 597, 863, 884
Msp20I TGGCCA 1 cut(s) 14
MspI CCGG 1 cut(s) 30
MspR9I CCNGG 5 cut(s) 432, 465, 504, 584, 1022
MunI CAATTG 2 cut(s) 16, 261
MvaI CCWGG 5 cut(s) 432, 465, 504, 584, 1022
MwoI GCNNNNNNNGC 4 cut(s) 95, 266, 549, 980
NlaIII CATG 8 cut(s) 96, 216, 252, 260, 596, 722, 750, 764
NlaIV GGNNCC 3 cut(s) 57, 581, 909
NspI RCATGY 1 cut(s) 260
OliI CACNNNNGTG 1 cut(s) 884
PaeI GCATGC 1 cut(s) 260
PagI TCATGA 1 cut(s) 760
PfeI GAWTC 4 cut(s) 119, 167, 232, 695
PflFI GACNNNGTC 1 cut(s) 803
PflMI CCANNNNNTGG 2 cut(s) 368, 485
PkrI GCNGC 4 cut(s) 91, 268, 291, 973
PleI GAGTC 2 cut(s) 672, 833
PpsI GAGTC 2 cut(s) 672, 833
Psp6I CCWGG 5 cut(s) 430, 463, 502, 582, 1020
PspGI CCWGG 5 cut(s) 430, 463, 502, 582, 1020
PspN4I GGNNCC 3 cut(s) 57, 581, 909
PspPI GGNCC 4 cut(s) 56, 96, 438, 908
PsrI GAACNNNNNNTAC 2 cut(s) 1001, 1033
PsyI GACNNNGTC 1 cut(s) 803
RsaI GTAC 3 cut(s) 107, 387, 1031
RsaNI GTAC 3 cut(s) 106, 386, 1030
RseI CAYNNNNRTG 3 cut(s) 597, 863, 884
SaqAI TTAA 5 cut(s) 525, 705, 714, 753, 819
SatI GCNGC 4 cut(s) 90, 267, 290, 972
Sau96I GGNCC 4 cut(s) 56, 96, 438, 908
ScaI AGTACT 1 cut(s) 1031
SchI GAGTC 2 cut(s) 673, 833
ScrFI CCNGG 5 cut(s) 432, 465, 504, 584, 1022
SduI GDGCHC 2 cut(s) 115, 679
SexAI ACCWGGT 2 cut(s) 463, 582
SfaNI GCATC 3 cut(s) 342, 416, 743
SinI GGWCC 2 cut(s) 56, 908
SmiMI CAYNNNNRTG 3 cut(s) 597, 863, 884
SmlI CTYRAG 2 cut(s) 519, 729
SmoI CTYRAG 2 cut(s) 519, 729
SphI GCATGC 1 cut(s) 260
Sse9I AATT 7 cut(s) 16, 63, 177, 197, 261, 526, 564
SspI AATATT 1 cut(s) 1048
SspMI CTAG 1 cut(s) 123
StyD4I CCNGG 5 cut(s) 430, 463, 502, 582, 1020
TaaI ACNGT 3 cut(s) 75, 417, 769
TaiI ACGT 2 cut(s) 306, 888
TasI AATT 7 cut(s) 16, 63, 177, 197, 261, 526, 564
TatI WGTACW 2 cut(s) 105, 1029
TfiI GAWTC 4 cut(s) 119, 167, 232, 695
Tru1I TTAA 5 cut(s) 525, 705, 714, 753, 819
Tru9I TTAA 5 cut(s) 525, 705, 714, 753, 819
TscAI CASTG 4 cut(s) 327, 420, 490, 772
TseI GCWGC 4 cut(s) 89, 266, 289, 971
TspDTI ATGAA 2 cut(s) 360, 849
TspRI CASTG 4 cut(s) 327, 420, 490, 772
Tth111I GACNNNGTC 1 cut(s) 803
Van91I CCANNNNNTGG 2 cut(s) 368, 485
VpaK11BI GGWCC 2 cut(s) 56, 908
XapI RAATTY 1 cut(s) 564
XceI RCATGY 1 cut(s) 260
XmiI GTMKAC 1 cut(s) 317
XspI CTAG 1 cut(s) 123
ZrmI AGTACT 1 cut(s) 1031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.